CONFIDENTIAL

** Contact with acid releases poisonous gas, H2S, use extreme caution when handling this
solution**
**Use caution when handling SAOB (2 M KOH, Highly Caustic) always wear gloves and
protective eye wear**

Part# 2010437 Title: LP2010437 Revision: B

Description: Sulfide Ion Stock Solution, 1000 ppm

1.0 Formulation:

1.1 0.03123 m Sodium Sulfide (Na2S·9H2O)

1.2 500 ml/L SOAB, Sulfide Anti Oxidant Buffer.

2.0 Materials:

2.1 7.490 ± 0.005 gm Na2S·9H2O (PN 9270117)

2.2 500 ml SOAB (PN 2010438)
2.3 1 liter Distilled Water
2.4 1 liter Plastic Volumetric Flask
2.5 Magnetic stirrer and stir bar
2.6 (2) 500 ml Glass Bottles
2.7 Avery Label 5164.

Teledyne Analytical Instruments – PO Box 121744, Z -19 SAIF Zone, Sharjah, UAE. PH# +971506345537
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 3.0 Procedure:

3.1 Open a new Lot # in the Laboratory Logbook, the Lot# will be the SO# or the WO# if no
SO# is available.
3.2 Record the chemical supplier, name, grade, Lot # and amount for each of the chemicals
used, example, Spectrum KCl, AR grade, Lot AE45321, 100.5 gm
3.3 Add 500 ml of SOAB to the 1000 ml flask
3.4 Weigh 7.490 ± 0.005 gm Na2S·9H2O and add it to the Flask.
3.5 Add 4-500 ml of distilled water and swirl to dissolve the salt.
3.6 Add distilled water to the 1000 ml mark and add the magnetic stir bar.
3.7 Stir until all salts are dissolved or at least 10 minutes.

4.0 Verification and Release:

4.1 Using a calibrated Sulfide ion electrode verify the solution is 1000 ± 5 ppm
4.2 Adjust if necessary and record the measured value and any adjustments in the Logbook
4.3 Pour the 1000 ppm Stock Solution into 500 ml bottles
4.4 Seal bottles with the screw on lids
4.5 Print labels with Lot# and Expiration date, 3 months from date of manufacture.
4.6 Place label slightly above the center of the bottle and level.
4.7 Release for use.

Revision
Revision ECR# Description Approval
Rev.A Rupert Original Procedure Dec. 14 ‘07
Rev.B Rupert 50% from 25% SAOB June 22, 2016

Teledyne Analytical Instruments – PO Box 121744, Z -19 SAIF Zone, Sharjah, UAE. PH# +971506345537
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