CELL RESPIRATION - MULTIPLE CHOICE QUESTIONS

1. Which is not a product of the Krebs cycle?

A. CO2

+
B. NADH + H

C. Pyruvate

D. ATP

2. How many ATP molecules (net yield) are produced per molecule of glucose as a direct result of
glycolysis?

A. 2

B. 4

C. 10

D. 38

3. Where in eukaryotic cells is glucose broken into pyruvate, to release energy for use in the cell?

A. Chloroplast

B. Cytoplasm

C. Mitochondrion

D. Nucleus

4. What is the net production of ATP, per molecule of glucose during the fermentation of glucose
to lactate?

A. 36 molecules

B. 4 molecules

C. 2 molecules

D. None

5. Humans can respire aerobically and anaerobically. Which are products of both aerobic cell
respiration and anaerobic cell respiration in humans?

A. Pyruvate and ATP

B. Pyruvate and lactate

C. ATP and carbon dioxide

D. Lactate and carbon dioxide

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6. What are the end products of aerobic cell respiration?

A. Carbon dioxide and ethanol

B. Lactate and ATP

C. Water, ATP and oxygen

D. Water, carbon dioxide and ATP

7. What is the correct sequence of chemicals produced in the anaerobic respiration pathway?

A. Lactate → pyruvate → ethanol

B. Ethanol → pyruvate → glucose

C. Glucose → lactate → pyruvate

D. Glucose → pyruvate → lactate

8. What is the key function of acetyl CoA in cellular respiration?

A. Involved in carbohydrate and fatty acid metabolism

B. Used in anaerobic respiration to oxidize pyruvate

C. Introduces amino acids into the Krebs cycle

D. Used as a hydrogen carrier in the link reaction

9. During which process are oxygen molecules directly involved during cellular respiration?

A. Glycolysis

B. Krebs cycle

C. Oxidation of pyruvate to acetyl CoA

D. Accepting electrons at the end of the electron transport chain

10. Which combination of changes describes biological oxidation?

Electrons Oxygen Hydrogen

A. loss gain loss
B. loss loss gain
C. gain loss gain
D. gain gain loss

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11. The diagram below shows the three stages of glycolysis. Which processes are indicated by I, II
and III?

6 -c a rb o n g lu c o s e

6 -c a rb o n g lu c o s e p h o s p h a te

II

3 -c a rb o n 3 -ca rb o n
s u g a r p h o s p h a te s u g a r p h o s p h a te

III

3 -c a rb o n 3 -ca rb o n
p y ru v a te p y ru v a te

I II III
A. Lysis Phosphorylation Oxidation and ATP
formation
B. Oxidation and ATP Phosphorylation Lysis
formation
C. Phosphorylation Lysis Oxidation and ATP
formation
D. Phosphorylation Oxidation and ATP Lysis
formation

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12. Within the mitochondria, what compound is produced through oxidation of fatty acids?

A. Acetyl-CoA

B. Acetylcholine

C. Oxaloacetate

D. Pyruvate

13. Which row in the table describes the first stage of cellular respiration?

Substrate Location Product Product

A. pyruvate mitochondria oxygen water
B. pyruvate cytoplasm carbon dioxide ATP
C. glucose mitochondria pyruvate water
D. glucose cytoplasm pyruvate ATP

14. Aerobic respiration involves conversion of glucose into pyruvate and conversion of pyruvate
into carbon dioxide and water. Where do these processes occur in a eukaryotic cell?

Where glucose is broken down Where pyruvate is broken down

into pyruvate into carbon dioxide and water
A. Cytoplasm Cytoplasm
B. Cytoplasm Mitochondrion
C. Mitochondrion Cytoplasm
D. Mitochondrion Mitochondrion

15. What is the “link reaction” in eukaryotic respiration?

+
A. Pyruvate joining with coenzyme A to produce CO2 and NADH + H

B. Oxidation of NADH to yield electrons and protons

C. Acetyl coenzyme A combining or joining with a C4 compound to give C6 + coenzyme A

D. Passage of acetyl coenzyme A through the mitochondrial membrane

16. Which of the following reactions is an oxidation reaction?

A. Pyruvate  Acetate + CO2

+ –
B. FAD + 2H + 2e  FADH2

C. Ribulose bisphosphate + CO2  2  Phosphoglycerate

+ + – +
D. NADP + 2H + 2e  NADPH + H

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17. The average surface area for the inner membranes of mitochondria in a epithelial cell is 40 m
–1
g . The surface area of the inner membrane of mitochondria from heart muscle cells is over 200
2 –1
m g .

What is the reason for the large surface area of the inner membranes of the mitochondria in the
heart muscle cells?

A. They contain enzymes to hydrolyse ADP and inorganic phosphate to ATP.

B. They contain enzymes to oxidize ADP and inorganic phosphate to ATP.

C. They contain enzymes to reduce ADP and inorganic phosphate to ATP.

D. They contain enzymes to condense ADP and inorganic phosphate to ATP.

18. Which of the following produce ATP in mitochondria?

A. The movement of protons from the matrix to the intermembrane space

B. The movement of protons from the intermembrane space to the cytoplasm

C. The splitting of water molecules and the movement of electrons to oxygen

D. The movement of protons from the intermembrane space to the matrix

19. What accumulates in the inter-membrane space of the mitochondrion during electron transport?

A. ATP

B. Electrons

C. Protons (hydrogen ions)

D. Oxygen

20. Which way do the protons flow when ATP is synthesized in mitochondria?

A. From the inner matrix to the intermembrane space

B. From the intermembrane space to the inner matrix

C. From the intermembrane space to the cytoplasm

D. From the cytoplasm to the intermembrane space

21. What is the sequence of stages during the conversion of glucose into pyruvate in glycolysis?

A. Lysis phosphorylation of sugar oxidation

B. Lysis oxidation phosphorylation of sugar

C. Phosphorylation of sugar lysis oxidation

D. Phosphorylation of sugar oxidation lysis

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22. How many molecules of acetyl CoA (ethanoyl CoA) does the oxidation of the fatty acid stearic
acid produce?

C H 3

C H 2

C H 2

C H 2

C H 2

C H 2

C H 2

C H 2

C H 2
S te a ric a c id
C H 2

C H 2

C H 2

C H 2

C H 2

C H 2

C H 2

C H 2

C O O H

A. 2

B. 6

C. 9

D. 18

ENZYME BIOLOGY - MULTIPLE CHOICE QUESTIONS

23. Which of the following reactions is an oxidation reaction?

A. Pyruvate  Acetate + CO2

+ –
B. FAD + 2H + 2e  FADH2

C. Ribulose bisphosphate + CO2  2  Phosphoglycerate

+ + – +
D. NADP + 2H + 2e  NADPH + H

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24. Consider the metabolic pathway shown below.

1 2 3 4
A B C D E

If there is end-product inhibition, which product (B to E) would inhibit which enzyme (1 to 4)?

Product Enzyme
A. C 4
B. B 3
C. B 4
D. E 1

25. Which statement describes how allosteric enzymes work?

Competitive End-product Active and

Reversible
inhibition inhibition inactive forms
A.
B.
C.
D.

Key: = yes = no

26. The diagram below shows the energy levels of a reaction in the presence or absence of an
enzyme. What is the best explanation of the different energy levels labelled I, II and III?

II
E n e rg y le v e l

E n e rg y o f
re a c ta n ts
III

E n e rg y o f p ro d u c ts

P ro g re s s o f re a c tio n

I II III
A. Absence of an enzyme Presence of an enzyme Endergonic reaction
B. Presence of an enzyme Absence of an enzyme Exergonic reaction
C. Absence of an enzyme Presence of an enzyme Exergonic reaction
D. Presence of an enzyme Absence of an enzyme Endergonic reaction
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27. What can reduce the effect of a competitive inhibitor of an enzyme?

A. Decrease the temperature at which the reaction takes place.

B. Increase the temperature at which the reaction takes place.

C. Increase the substrate concentration.

D. Add a non-competitive inhibitor.

28. What effect do enzymes have on the activation energy of exergonic and endergonic reactions?

Activation energy of Activation energy of

exergonic reactions endergonic reactions
A. increases increases
B. decreases decreases
C. increases decreases
D. decreases increases

29. According to the induced fit model of enzyme function, which of the following statements is
correct?

A. Active sites on enzymes are specific to a single substrate.

B. The shape of the active site can be changed by the binding of an allosteric inhibitor.

C. The binding of the substrate changes the shape of the active site slightly.

D. Competitive inhibitors can change the shape of enzymes.

30. In the enzyme controlled pathway shown below, which compound is most likely to inhibit
enzyme (w)?

enzyme enzyme enzyme enzyme

w x y z
Precursor  I  II  III  IV

A. I

B. II

C. III

D. IV
(1)

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31. The graphs below show the energy changes during endergonic and exergonic reactions, with
and without enzymes.

Which line represents an endergonic reaction, without an enzyme?

A. I

B. II

C. III

D. IV

32. Which graph shows the relationship between the substrate concentration and the rate of an
enzyme controlled reaction?

A . B.
re a c tio n
R a te o f
re a c tio n
R a te o f

S u b s tr a te S u b s tra te
c o n c e n tra tio n c o n c e n tra tio n

C. D .

(Total 1 mark)