Sep. 2017
Prominence-i
LC-2030 Plus
LC-2030C Plus
LC-2030C 3D Plus
Integrity Guide
Read this manual thoroughly before you use the product.
Keep this manual for future reference.
This page is intentionally left blank.
Introduction
Read this Instruction Manual thoroughly before using
the product.
Thank you for purchasing this product.
This manual describes the information about "Testing" for verifying that this product
meets "CHECK CRITERIA" and about "calibration" for adjustment so that this product
meets the CHECK CRITERIA, in order to secure the reliability of analysis data. Read this
manual thoroughly before using the product and operate the product in accordance with
the instructions in this manual. The following instruction manuals are included with the
product. PDF documents are included in the instruction manual CD-ROM.
System Guide Describes the basic operation and troubleshooting for this
(Booklet/PDF) product.
This instruction manual. Describes "validation" for verifying that
Integrity Guide this product meets "CHECK CRITERIA" and "calibration" for
(PDF) adjustment so that this product meets the CHECK CRITERIA, in
order to secure the reliability of analysis data.
Describes the operation of the operations screen and the
Operation Guide
operational method for the Web screen, iPhone, and iPad for
(PDF)
this product.
Maintenance Guide Describes the maintenance for this product, including
(PDF) inspection, part replacement, and rinsing.
Read "Introduction" in this section thoroughly before using the product. "Introduction"
describes the information about the warranty, after-sales service, safety instructions and
precautions to ensure safe operation of the instrument.
For information on the operation, hardware validation, and accessories and options for
this product, refer to "System Guide", "Operation Guide" and "Maintenance Guide".
Keep this manual for future reference.
Important
• If the user or usage location changes, ensure that this manual is always kept
together with the product.
• If this manual or a product warning label is lost or damaged, immediately contact
your Shimadzu representative to request a replacement.
• To ensure safe operation, read "Safety Instructions" P.iv thoroughly before using
the product.
• To ensure safe operation, contact your Shimadzu representative if product
installation, adjustment, re-installation (after the product is moved), or repair is
required.
Notice
• Information in this manual is subject to change without notice and does not represent
a commitment on the part of the vendor.
• Any errors or omissions which may have occurred in this manual despite the utmost
care taken in its production will be corrected as soon as possible, although not
necessarily immediately after detection.
• All rights are reserved, including those to reproduce this manual or parts thereof in
any form without permission in writing from Shimadzu Corporation.
• Some portions of illustrations in this manual might be different from the instrument
used.
• Microsoft and Windows are registered trademarks of Microsoft Corporation in the
United States and/or other countries.
iPhone and iPad are registered trademarks of Apple Inc. in the United States and/or
other countries.
Other company names and product names mentioned in this manual are trademarks or
registered trademarks of their respective companies. The ä and â symbols are omitted
in this manual.
• Prominence-i is registered trademarks of Shimadzu Corporation in the United States and
Europe (Office for Harmonization in the Internal Market).
Indication Meaning
Indication Meaning
Safety Instructions
To ensure safe product operation, read these important safety instructions carefully
before use and follow all WARNING and CAUTION instructions given in this section.
n Product Applications
n Installation Site
Avoid installation sites that are exposed to corrosive gases or excessive dust.
These adverse conditions may be detrimental in maintaining the instrument's
performance and may shorten its service life.
During installation, consider the entire mass of this system combined with
other components.
• Model without a sample cooler: 58 kg
• Model with a sample cooler: 63 kg
The mass of this system is as described above. Please set up this instrument in
consideration of the entire mass combined with other components, PC, the
monitor, and the printer, etc.
"7 Technical Information" in the System Guide
Install the product in locations where the power switch can be easily operated.
The power switch must be turned off in case of an emergency.
n Installation
n Operation
Before removing any high pressure flow line part, such as tubing, the sample
loop, the needle, and the needle seal, follow the safety precautions below:Be
sure to stop the pump and confirm the pressure of the mobile phase has fallen
to zero.
Removing any of these parts while high pressure remains could cause the
mobile phase and the clogging particles to eject rapidly and injury could occur.
Confirm that the pressure has fallen to zero before removing these parts.
If the pressure has not fallen to zero, the high-pressure flow line might be
clogged. After confirming the end of the drain tubing is in the waste container,
open the drain valve and release the pressure of the mobile phase.
Do NOT bend any tubing of the instrument at the same position repeatedly.
Otherwise, a rupture or cracks of the tubing are likely to occur, and might
result in solvent leaks.
Do NOT allow spilled water to remain on the instrument surface, and do not
use alcohol or thinner-type solvents to clean the surfaces.
Otherwise, these can cause rusting and discoloration.
If the power cord plug gets dusty, remove the plug from the power outlet
and wipe away the dust with a dry cloth.
Otherwise, fire may occur.
Dispose of the waste liquid properly and in accordance with the instruction
by your administrative department.
n In an Emergency
If any problem is detected, such as a burning smell, take the following action:
When the instrument is used again, inspect the instrument and, if necessary, contact your
Shimadzu representative to request servicing.
2 After confirming all related items in this section "Installation" P.vi and "Operation"
P.viii, use the standard startup procedure to start the instrument.
Generation of static When liquid is passed through thin tubing at high flow
electricity rates, as in liquid chromatograph, the electrostatic charges
of the flowing matter generate static electricity.
The best way to prevent static electricity accidents is simply to prevent the occurrence
and accumulation of electrostatic charges.
n Preventive Measure 1
Use a metal container for the waste liquid, and ground the container.
This will ensure that the electrical charges of the container and liquid pass to the ground.
Be sure to verify, using a tester, that the waste container is properly grounded.
Some metal containers have surfaces that are oxidized or laminated. Such a
container may not conduct electricity.
n Preventive Measure 2
Cover the spaces between the tubing and the sides of the inlet and outlet openings of
the waste container with caps or other protective covering. This will prevent any sparks
generated outside the container from getting inside.
n Preventive Measure 3
Keep electrostatically charged objects, including the human body, away from the waste
liquid container.
To prevent electrostatic charging of the human body, take the following precautions:
• Wear anti-static clothing and shoes.
• Ground the human body with anti-static wrist straps. (For safety, the wrist strap should
be connected to the ground using an intervening resistor of about 1 MW.)
• Spread anti-static matting or the like on the floor, to make the floor conductive.
Persons who have not taken anti-static precautions should touch some
grounded metal object before coming near the waste liquid container, in order
to drain static charges.
n Preventive Measure 4
Use tubing with an inner diameter of at least 2 mm for drain lines with high flow rates.
n Preventive Measure 5
If it is not possible to use a conductive waste liquid container, take the following
precautions:
Ensure that the end of the inflow tubing is always submerged inside the container. Also,
place some type of grounded metal object, such as a ground wire connected to the
instrument, into the liquid.
For low conductivity (less than 10-10 S/m) liquids, take preventive measures 1
to 4.
For low conductivity (less than 10-10 S/m) liquids, preventive measure 5 has no
effect.
Use as small a container as possible to minimize damage in the event of fire.
For Reference
Anti-static equipment (anti-static clothing, shoes and matting) and charge measurement
equipment (potentiometer) are sold by specialty manufacturers.
Do NOT use resin parts for the high-pressure tubing while pumping at high
pressures.
Pumping at high pressure may cause resin tubing to be ruptured or
disconnected, which could result in mobile phase leaks.
Please note the maximum withstand pressure of each part when resin parts
are used for the high-pressure tubing.
Do NOT use highly volatile acids, such as acetic acids in high concentration
(10 % to 50 %) or 1 % TFA (trifluoroacetic acid) solution, as a mobile phase
or rinse solution of the autosampler continually.
Doing so could cause metallic parts in the instrument to corrode. If such liquids
have been used for analysis, purge the mobile phase or rinse solution from the
flow line with distilled water or other liquid that is less corrosive. Also, turn
the autosampler off after analysis and open the front door slightly to let the
vapor release from the inside of the instrument.
The following solvent could damage the system. Never use these solvents in
the degassing unit.
If PEEK resin parts are used for tubing connections, do not use the following
mobile phases.
These mobile phases weaken PEEK resin, which may lead to cracked tubing and
mobile phase leaks.
• Use only HPLC grade or comparable mobile phase, and filter it with a filter
of 0.45 µm mesh or finer before use to remove particulates and foreign matter.
• Halogen ions can corrode the stainless steel material (SUS316L) used in the
plumbing, so if such materials are used for the wetted parts of the equipment,
avoid as much as possible mobile phases that contain halogen ions, including
KCl, NaCl and NH4Cl or mobile phases that generate halogen ions in certain
reactions. If such mobile phases must be used, clean all flow lines thoroughly
with distilled water immediately after analysis.
• Avoid ammonia aqueous solution at a concentration exceeding 0.1 %. It may
damage the plunger of the pump and shorten the service life of the plunger
and the plunger seal.
• When an absorbance detector or photodiode array detector is used for
high-sensitivity analysis, be sure to use HPLC grade mobile phases that have a
low absorptivity of UV rays.
• Always degas the mobile phase, as air bubbles may tend to form during solvent
mixing or during temperature or pressure changes. Air bubbles may cause
pump malfunctions and detector signal noise.
• Understand the properties, including boiling points, firing point and viscosities,
of the mobile phase.
Warning Labels
In order to ensure safety, warning labels are attached in places requiring caution.
If a warning label is lost or damaged, obtain a new label through your Shimadzu
representative and attach it in the correct position.
Warning
No. Description
Label
(Part No. S037-72999-02)
1 During operations such as calibration, the autosampler operates even
with the front panel open. Do NOT touch the needle moving parts.
Warning
No. Description
Label
(Part No. S228-53303)
If a drain tube is not connected, the rinse solution may leak,
6 resulting in malfunction of the instrument. When a model with a
sample cooler is used, the condensation water may leak, resulting
in malfunction of the instrument.
(Part No. S228-53303)
7 If a drain tube is not connected, leaked solutions will not drain,
resulting in malfunction of the instrument.
(Part No. S228-53303)
If the column oven temperature is set to below the room
8 temperature, condensation may occur. If a drain tube is not
connected, leakage may occur, resulting in malfunction of the
instrument.
(Part No. S228-53303)
9 Be careful of dirt on the air filter. If the filter is clogged with dust,
the instrument's performance may be impaired.
Warranty
Shimadzu provides the following warranty for this product.
1. Period:
Please contact your Shimadzu representative for information about the period of this warranty.
2. Description:
If a product/part failure occurs for reasons attributable to Shimadzu during the warranty period,
Shimadzu will repair or replace the product/part free of charge. However, in the case of products
which are usually available on the market only for a short time, such as personal computers and
their peripherals/parts, Shimadzu may not be able to provide identical replacement products.
3. Limitation of Liability:
(1) In no event will Shimadzu be liable for any lost revenue, profit or data, or for special, indirect,
consequential, incidental or punitive damages, however caused regardless of the theory of
liability, arising out of or related to the use of or inability to use the product, even if Shimadzu
has been advised of the possibility of such damage.
(2) In no event will Shimadzu's liability to you, whether in contract, tort (including negligence), or
otherwise, exceed the amount you paid for the product.
4. Exceptions:
Failures caused by the following are excluded from the warranty, even if they occur during the
warranty period.
(2) Repairs or modifications performed by parties other than Shimadzu or Shimadzu designated
companies
(3) Product use in combination with hardware or software other than that designated by Shimadzu
(4) Computer viruses leading to device failures and damage to data and software, including the
product's basic software
(5) Power failures, including power outages and sudden voltage drops, leading to device failures
and damage to data and software, including the product's basic software
(6) Turning OFF the product without following the proper shutdown procedure leading to device
failures and damage to data and software, including the product's basic software
(8) Product use in harsh environments, such as those subject to high temperatures or humidity levels,
corrosive gases, or strong vibrations
(9) Fires, earthquakes, or any other act of nature, contamination by radioactive or hazardous
substances, or any other force majeure event, including wars, riots, and crimes
* If there is a document such as a warranty provided with the product, or there is a separate contract
agreed upon that includes warranty conditions, the provisions of those documents shall apply.
If any problem occurs with this product, perform an inspection and take appropriate
corrective action as described in "6 Troubleshooting" of the System Guide.
If the problem persists, or the symptoms are not covered in the troubleshooting section,
contact your Shimadzu representative.
Replacement parts for this product will be available for a period of seven (7) years after
the product is discontinued. Thereafter, such parts may cease to be available.
Note, however, that the availability of parts not manufactured by Shimadzu shall be
determined by the relevant manufacturers.
n Hardware Validation
Each unit in the instrument and the entire LC system should be checked periodically to
ensure that they function normally, else the analysis data may not be reliable.
Therefore, it is necessary to carry out periodic hardware validation and keep records of
the validation.
Before shipment from the factory, this instrument was rigorously inspected. The results
are summarized in the Inspection Certificate accompanying the instrument. To inspect the
instrument performance after installation, repeat the Hardware Validation as described
in "2 Validation" P.2.
Disposal Precautions
When disposing of the instrument, contact your Shimadzu representative.
If you dispose them yourself, do so in accordance with the processing standards
determined by law, separately from general industrial waste and household garbage.
If the deuterium (D2) lamp should be broken or its life is finished, dispose of the lamp
separately from general garbage. When disposing of the deuterium (D2) lamp provided
from Shimadzu Corporation, select a method, which will not harm the environment or
cause bodily injury. Consult your local Government Agencies for a proper disposal method.
1 Overview
2.2.2 Processing the System Check Using PC, iPad, or iPhone ..... 8
2.2.3 List of System Check Results ................................. 12
n Types of validation
Validations using the instrument include performance checks and automatic validations
processed from the instrument's Operations screen and validations processed using
external processing instruments. System check allows the user to list and check validation
results or the operating conditions of consumable parts.
The user can also check the histories of automatic validations and performance checks via
the [Maintenance] screen in the [Log] tab. The user can also check the operating condition
of a consumable part on the [Maintenance] screen.
The user should document and record the results of conducted checks for later
reference. The user can save the automatic validation and performance check
results from the PC Web screen and print them from the application software.
n Validation items
The items that could not be tested through automatic validation can be tested through
performance checks to reduce the system check time.
1 Press (Menu).
2 Press [Maintenance].
The [Maintenance] screen is displayed.
3 Press (Validation).
The user can process the check from a PC, iPad, or iPhone in the same network.
For the setting procedure of your PC, iPad, or iPhone, refer to the Operation Guide.
Operation Guide "4.9 Check the Status of the Instrument from PC", "4.10 Check the Status
of the Instrument from iPad", and "4.11 Check the Status of the Instrument from
iPhone"
1 Display the Web screen on a PC, iPad, or iPhone in the same network.
6 Click on [OK].
The [System Check Running] screen is
displayed and the system check is started.
n Instrument information
Displays the information for the instrument relevant to the system check.
"2.2.3 List of System Check Results" P.12
of each model and replace the consumable part or try to resolve the cause of
the failure.
• Powering off the instrument causes only [System Check Result] to be saved and
the data for [Unit Info] and [Details] to be erased.
Displayed items vary between UV detector system models and PDA detector system
models.
26
Comp.Factor Correction value of a gradient concentration calibrated with 2
A/B 90% mobile phase port A at 10 % and B at 90 %.
Comp.Factor Correction value of a gradient concentration calibrated with
27
A/C 10% mobile phase port A at 90 % and C at 10 %.
Comp.Factor Correction value of a gradient concentration calibrated with
28
A/C 50 % mobile phase port A at 50 % and C at 50 %.
Comp.Factor Correction value of a gradient concentration calibrated with
29
A/C 90 % mobile phase port A at 10 % and C at 90 %.
Comp.Factor Correction value of a gradient concentration calibrated with
30
A/D 10 % mobile phase port A at 90 % and D: at 10 %.
Comp.Factor Correction value of a gradient concentration calibrated with
31
A/D 50 % mobile phase port A at 50 % and D at 50 %.
Comp.Factor Correction value of a gradient concentration calibrated with
32
A/D 90 % mobile phase port A at 10 % and D at 90 %.
33 [Performance Check] -
Displays the results of system checks for [Flow rate accuracy]
as follows.
[Measurement condition (mL/min)] [Measured value (%)] < / >
Flow Accuracy
34 [Check criteria (%)]
/±%
Display example: (1.5 mL) -0.17 < 0.5
* When the measured value at 1.5 mL/min was -0.17% with the
CHECK CRITERIA at ±0.5 %
Sample Loop Displays the total times the sample loop was used as follows
60
/time [Total usage] < / > [Check criteria]
61 [Calibration] -
Value of the compensation factor by running calibration for
62 Inj. Accuracy Factor
[Injection Volume accuracy].
Rack Pos. Latest date and time at which rack position adjustment was
63
Adjustment processed.
Latest date and time at which injection port position
64 Inj. Port Adjustment
adjustment was processed.
65 [Performance Check] -
Displays the result of the system check for [Injection Volume
66 Inj. Accuracy /±% accuracy] as follows.
[Measured value (%)] < / > [Check criteria (%)]
67 - -
68 <OVEN UNIT> -
Displays the CMD (Column Management Device) connection as
69 CMD
[ON] (connected) or [OFF] (not connected).
70 [Check-Consumables] -
Displays the total times the flow line switching valve has
Valve (Seal) rotated as follows. If no flow line switching valve is connected,
71 [NONE] is displayed.
/rev
[Total number of revolutions] < / > [Check criteria]
72 [Calibration] -
Displays the result of the calibration run for [Temperature
accuracy] as follows.
[1st inspection temperature (°C)][1st measured value]
73 Temp.Comp.1 /°C
Display example: (25) 26.2
* When the first inspection temperature is set to 25 °C and the
external thermosensor measurement is 26.2 °C
79 Gas Sensor Test Results of system checks for [Gas Sensor Test].
80 - -
*1 The value varies depending on the instrument (holmium oxide filter). The lambda max wavelength
is displayed in [Measurement condition (nm)].
ACCTIME W
Displays the exposure time of the W lamp (when using an 8 2
95 nm slit).
WID /ms
Not displayed if no W lamp is connected.
96 [Performance Check] -
97 Slit Check Results of system checks for the slit.
Displays the number of times the user switched between a
98 Slit Switch Times
1.2 nm slit and an 8 nm slit.
Displays the results of system checks for [Lamp energy] for the
D2 Energy D2 lamp (when using a 1.2 nm slit) as follows.
99
NAR(230nm) /V
[Measured value (V)] < / > [Check criteria (V)]
Displays the results of system checks for [Lamp energy] for the
D2 Energy D2 lamp (when using an 8 nm slit) as follows.
100
WID(230nm) /V
[Measured value (V)] < / > [Check criteria (V)]
Displays the results of system checks for [Lamp energy] for the
W Energy W lamp (when using a 1.2 nm slit) as follows. Not displayed
101 if no W lamp is connected.
NAR(600nm) / V
[Measured value (V)] < / > [Check criteria (V)]
Displays the results of system checks for [Lamp energy] for the
W Energy W lamp (when using an 8 nm slit) as follows.
102 Not displayed if no W lamp is connected.
WID(600nm) /V
[Measured value (V)] < / > [Check criteria (V)]
WAV. Acc. 656.1 Testing results with [Wavelength accuracy] at a center
103
nm(NAR) wavelength (656.1 nm) (when using a 1.2 nm slit).
WAV. Acc. 486.0 Testing results with [Wavelength accuracy] at a center
104
nm(NAR) wavelength (486.0 nm) (when using a 1.2 nm slit).
WAV. Acc. 360.8 Testing results with [Wavelength accuracy] at a center
105
nm(NAR) wavelength (360.8 nm) (when using a 1.2 nm slit).
WAV. Acc. 241.5 Testing results with [Wavelength accuracy] at a center
106
nm(NAR) wavelength (241.5 nm) (when using a 1.2 nm slit).
WAV. Acc. 656.1 Testing results with [Wavelength accuracy] at a center
107
nm(WID) wavelength (656.1 nm) (when using an 8 nm slit).
Wavelength
108 Results of system checks for [Wavelength accuracy].
Accuracy
Displays the results of system checks for [Baseline Noise/Drift]
109 Noise /mAU as follows.
[Measured value (mAU)] < / > [Check criteria (mAU)]
The user can save the results of system checks on a PC as a text file. The saved data can
be viewed and edited in an application like Excel.
• For the setting procedure of your PC, refer to the Operation Guide.
• The user cannot save the results on an iPad or iPhone.
Operation Guide "4.9 Check the Status of the Instrument from PC", 4.10 Check the Status 2
of the Instrument from iPad", and "4.11 Check the Status of the Instrument from
iPhone"
1 Display the Web screen on a PC, iPad, or iPhone in the same network.
A filename is automatically
assigned.
7 Click on [Save].
2
During automatic validation, keep the room temperature fluctuation within the
range from +1 °C max. to -1 °C min.
The baseline may fluctuate.
Items that were not tested through automatic validation can be tested through performance
checks to improve the efficiency of the test.
Process the automatic validation from the [Validation] tab in the [Maintenance] screen.
Prepare the following parts before the test.
2 Press [Maintenance].
The [Maintenance] screen is displayed.
3 Press (Validation).
• GRAD. mode
10 Press [Next].
11 Press [Start].
Automatic validation is started.
• ISO mode
Automatically purge the selected mobile
phase ports and then conduct the tests
from [2. Wavelength] to [9. Pressure
Limits].
• GRAD. mode
Automatically purge all the mobile phase
ports and then conduct the tests from [2. Wavelength] to [10. Gradient].
Press [Stop] to cancel the test. If [OK] is pressed on the Confirmation screen, the
tests are cancelled. If [Cancel] is pressed, the tests are restarted.
D2 Lamp The life of the lamp has ended. Replace with a new 2
one. If the situation persists even after replacing
with a new one, contact your Shimadzu sales office
Results of system or representative.
checks for lamp
W Lamp energy. Maintenance Guide
"5.5 Replacing the D2 Lamp"
"6.5 Replacing the D2 Lamp"
"6.6 Replacing the W Lamp (Optional Part)"
Results of system
Refer to "6.1 Failure Diagnoses and Actions" in the
Pulsation checks for pumping
System Guide.
stability.
Results of system
checks for Refer to "6.1 Failure Diagnoses and Actions" in the
Temperature
temperature control System Guide.
precision.
Results of system
Drift checks for baseline
drifting. Refer to "6.1 Failure Diagnoses and Actions" in the
Results of system System Guide.
Noise checks for detector
baseline noise.
Results of system
Refer to "6.1 Failure Diagnoses and Actions" in the
Pressure Limits checks for the
System Guide.
pressure limiter.
Check result of Calibrates the "gradient concentration accuracy".
gradient
Gradient "Gradient concentration accuracy calibration"
concentration
accuracy. P.109
This section explains how to display each item on the [Performance Check] screen.
1 Press (Menu).
2 Press [Maintenance].
The [Maintenance] screen is displayed.
3 Press (Validation).
The instructions vary depending on the item. The following is an explanation using [Flow
rate accuracy] as an example.
2.4.2 Pump
A mobile phase (water) is pumped at a set flow rate. Use a stopwatch to record the
time it takes to pump 5 mL. If the recorded time is entered by the user, the flow rate
accuracy is measured.
CHECK CRITERIA
±2.00 %
2 Press the [Pump] tab and then press [Flow rate accuracy].
The [Flow rate accuracy] screen is displayed.
5 Press [Next].
2
7 Press [Start].
Purging starts and the screen showing the
purge progress is displayed.
12 Remove the resistor tube and close the column oven door.
This test pumps a mobile phase (water) at 1 mL/min, monitors the pressure fluctuation
for 1 minute, and then measures the maximum pressure fluctuation margin.
CHECK CRITERIA
0.20 MPa max.
Measurement conditions
Item Requirements
Flow Rate 1 mL/min
Oven Temperature 40 °C
6 Press [Next].
8 Press [Start].
Purging starts and the screen showing the
purge progress is displayed.
10 Press [Next].
The results of system checks are displayed.
This test uses water and caffeine solution to perform binary low-pressure gradient.
Absorbance for each set concentration is measured and the concentration accuracy is
calculated.
CHECK CRITERIA
±2.00 % max.
Measurement conditions
Item Requirements
Flow Rate 1 mL/min
Wavelength 272 nm
Oven Temperature 40 °C
A/B, A/C, or A/D 100/0 % for 10 min
A/B, A/C, or A/D 90/10 % for 5 min
Gradient Program A/B, A/C, or A/D 50/50 % for 5 min
A/B, A/C, or A/D 10/90 % for 5 min
A/B, A/C, or A/D 0/100 % for 5 min
Select the desired ports to be used from the combinations A/B, A/C, or A/D. Set
a 10 mg/L caffeine solution for mobile phase port A, and set water for mobile
phase ports B, C, D, and the rinse solution port. (Fill those ports that will not
be tested with water.)
6 Press [Next].
8 Press [Next].
9 Press [Start].
Rinsing starts.
10 Press [Next].
The results of system checks are displayed.
This test first pumps a mobile phase (water) at 1 mL/min and then measures the pressure
when pumping stabilizes. Let this value be "X". After measurement, the upper limit
limiter is automatically set to (X+1) MPa, and the lower limit limiter is set to (X-1) MPa.
After that, the flow rate is changed to 1.5 mL/min and then 0.5 mL/min sequentially,
and check that the upper limit limiter and lower limit limiter are checked for normal
operation.
CHECK CRITERIA
The upper limit limiter and the lower limit limiter should operate normally.
6 Press [Next].
8 Press [Start].
Measurement starts and the screen
showing the measurement progress is
displayed.
When measurement finishes, the check
results are displayed.
2.4.3 Autosampler
This test uses a 1.5 mL sample vial containing a sample (water) to measure the mass
before and after 10 repeated injections of 50 µL. Based on the difference in mass, the
injection volume accuracy is calculated.
CHECK CRITERIA
±1.3 % max.
• For models with a sample cooler, turn OFF the temperature control of the
sample cooler to prevent condensation within the autosampler. For the setting
procedure, refer to "2.1 Analysis Screen" in the Operation Guide.
• Before testing, thoroughly flush the mobile phase port to be used and the
rinse solution with water (HPLC grade).
• When the optional sample loop for 50 µL injections is used, set the sample
aspiration rate to 5 µL/sec or less.
2 Press the [Autosampler] tab and then press [Injection Volume accuracy].
The [Injection Volume accuracy] screen is displayed.
6 Press [Next].
This test uses an external thermosensor to measure the temperature of the sample cooler.
If the measured temperature is entered by the user, the temperature accuracy of the
sample cooler is calculated. The set value of the sample cooler is "4 °C".
CHECK CRITERIA
±3.0 °C max.
2 Press the [Autosampler] tab and then press [Cooler Temperature accuracy].
The [Cooler Temperature accuracy] screen is displayed.
4 Press [Next].
The sample cooler starts temperature
control at a set temperature of 4 °C.
This test lets the user select the oven temperature from among 40 °C, 50 °C, and room
temperature -5 °C, and, once the temperature stabilized, measure the temperature of the
column holder using an external thermosensor. The oven temperature accuracy and
fluctuation are measured from the automatic measurement result and the measured
temperature of the temperature fluctuation.
The user can check the room temperature in the monitor value display screen.
CHECK CRITERIA
• Temperature accuracy: ±2.0 °C max.
2 Press the [Oven] tab and then press [Oven Temperature accuracy/fluctuation].
The [Oven Temperature accuracy/fluctuation] screen is displayed.
If necessary, tape at
two or more points
to ensure that the
external
thermosensor will
not fall off the
column holder.
4 Press [Next].
6 Press [Next].
7 Press [Next].
The next screen is displayed.
This test checks whether the gas sensor can detect organic solvent in the column oven.
Measurement conditions
Item Requirements
Oven Temperature 50 °C
4 Press [Next].
2
The following screen is displayed, and
temperature control starts.
When the temperature stabilizes, the
message "Press "Next" button to start
measurement." is displayed.
Press [Next].
6 Press [Next].
The results of system checks are displayed.
2.4.5 UV Detector
This test is used to measure the difference between the preset wavelength and the
measured wavelength. The lambda max*1 of the built-in filter (holmium oxide filter) and
the 656.1 nm emission line of the D2 lamp (deuterium (D2) lamp) are measured, and the
instrument automatically assesses whether both are within ±1.0 nm.
The D2 lamp energy is also checked.
*1 The value varies depending on the instrument (holmium oxide filter). For the lambda max
wavelength, see the criteria (240.4 nm to 241.7 nm) in the system check result screen of Procedure
7.
2
CHECK CRITERIA
• Wavelength accuracy: ±1.0 nm max.
2 Press the [Detector] tab and then press [Wavelength accuracy/Lamp energy].
The [Wavelength accuracy/Lamp energy] screen is displayed.
4 Press [Start].
Purging starts and the screen showing the
purge progress is displayed.
6 Press [Next].
The results of system checks are displayed.
This test measures absorbances when the built-in filter is OFF and ON and uses the
difference between these two absorbances and the absolute absorbance inherent to the
built-in filter in order to calculate the absorbance accuracy of the detector.
CHECK CRITERIA
±3.0 % max.
5 Press [Start].
Purging starts and the screen showing the
purge progress is displayed.
7 Press [Next].
The results of system checks are displayed.
This test measures the absorbance twice, once with the external standard filter not
mounted and once with the filter mounted. Based on the difference and the absolute
absorbance of the external standard filter, the absorbance accuracy of the detector is
calculated.
CHECK CRITERIA
±3.0 % max.
6 Press [Next].
Absorbance (background absorbance) with
the filter not mounted is adjusted to "0"
and the [Filter Installing] screen is
displayed.
8 Press [Start].
The next screen is displayed, starting
measurement of absorbance using the
standard absorbance filter (external
standard filter).
9 Press [Next].
The results of system checks are displayed.
This test measures the baseline noise and drift for 15 minutes after the baseline stabilizes.
CHECK CRITERIA
• Drift (when solvent is being delivered): 0.60 mAU/h
• Noise (when solvent is being delivered): 0.0200 mAU
Measurement conditions
Item Requirements
Flow Rate 1 mL/min
Oven Temperature 40 °C
Wavelength 250 nm
Response 2 sec
Cell Temperature 40 °C
6 Press [Next].
The next screen is displayed.
8 Press [Start].
Purging starts and the screen showing the
purge progress is displayed.
9 Press [Next].
The results of system checks are displayed.
"4.3 Wavelength Accuracy Testing," "4.4 Checking the Intensity of the Light" in the Help
section of the PDA Utility
This test is used to measure the difference between the preset wavelength and the
measured wavelength. Using the emission line of the D2 lamp (deuterium (D2) lamp) and
the built-in filter (holmium oxide filter) lambda max, the wavelength accuracy is checked.
The D2 lamp intensity and W lamp intensity (when an optional W lamp is connected) is
also checked.
Item Wavelength
241.5 nm
Holmium Oxide Filter Lambda Max
360.8 nm
486.0 nm
Emission Lines of the D2 Lamp
656.1 nm*
The above wavelengths assume that a 1.2 nm slit is used. When an 8 nm slit is
used, only the wavelength marked with * is checked. (The 1.2 nm and 8 nm slits
are automatically changed.)
CHECK CRITERIA
• Wavelength accuracy: ±1.0 nm max.
2
2 Press the [Detector] tab and then press [Wavelength accuracy/Lamp energy].
The [Wavelength accuracy/Lamp energy] screen is displayed.
4 Press [Start].
Purging starts and the screen showing the
purge progress is displayed.
6 Press [Next].
The results of system checks are displayed.
This test measures the absorbance twice, once with the external standard filter not
mounted and once with the filter mounted. Based on the difference and the absolute
absorbance of the external standard filter, the absorbance accuracy of the detector is
calculated.
Wait for 90 minutes or more after turning on a lamp. Because absorbance is not
stabilized immediately after lamp lighting.
2
CHECK CRITERIA
±3.0 %max.
5 Press [Next].
Absorbance (background absorbance) with
the filter not mounted is adjusted to "0"
and the [Filter Installing] screen is
displayed.
7 Press [Start].
The next screen is displayed, starting
measurement of absorbance using the
standard absorbance filter (external
standard filter).
8 Press [Next].
The results of system checks are displayed.
After the check, remove the standard absorbance filter (external standard filter)
immediately. The filter may be damaged.
This test measures the baseline noise and drift for 15 minutes after the baseline stabilizes.
Criteria
• Drift (when solvent is being delivered): 2.00 mAU/h max.
• Noise (when solvent is being delivered): 0.0500 mAU
Measurement conditions
Item Requirements
Flow Rate 1 mL/min
Oven Temperature 40 °C
Wavelength 250 nm
Bandwidth ±4 nm
Reference Correction OFF
Slit Width 8 nm
2
Sampling 2 sec
Time Constant 2 sec
Cell Temperature 40 °C
6 Press [Next].
The next screen is displayed.
8 Press [Start].
Purging starts and the screen showing the
purge progress is displayed.
9 Press [Next].
The results of system checks are displayed.
2.4.7 Others
There are cases where the leak sensor sensitivity is not optimal due to the influence of
changes in operating environment.
Check the leak sensor sensitivities of the detector, pump, and autosampler (HPV
(high-pressure valve) and LPV (low-pressure valve)).
Pump
Autosampler (HPV)
Autosampler (LPV)
5 Press [Start].
Start the measurement.
6 Press [Next].
The results of system checks are displayed.
The Analysis Reproducibility Test is a test to judge whether the cause of any trouble lies
in the instrument or if the problem is inherent to each analysis.
If trouble occurs, first conduct this test, and, depending on the result, take action as
follows.
In addition, conduct this test during a periodic inspection, too, in order to check if the
instrument operates normally.
n Isocratic analysis
This test checks if reproducible data can be obtained from an actual analysis.
This is determined from the coefficients of variation for the peak area and the retention
time of each peak.
Criteria
• "Retention time" coefficient of variation: 0.5 % max.
• "Peak area" coefficient of variation: 1.0 % max.
Before starting the test, rinse the flow line depending on the operating condition
of the instrument.
1 Instead of the column, connect the column inlet and outlet tubes to the PEEK
tubing using a coupling.
5 Put 1.0 mL of water in a sample vial, add a new septum, and close the cap.
System Guide "3.7 Setting a Sample"
6 Put 1.0 mL of sample in another sample vial, add a new septum, and close the
cap.
System Guide "3.7 Setting a Sample"
7 Start the temperature adjustment of the column oven and begin pumping for
the solvent delivery module.
Check that liquid is flowing from the outlet tubing of the detector and that there is no
fluid leaking from the connection part.
8 Monitor the baseline, and, if a stable baseline is obtained, conduct the zero
adjustment for the detector.
11 From the six analysis results, calculate the retention time of the peak and the
relative standard deviation (coefficient of variation) of the areas.
: Analysis count
: Mean
This test checks if reproducible data can be obtained from an actual analysis.
This is determined from the coefficients of variation for the peak area and the retention
time of each peak.
As an example, this section explains the gradient analysis of two liquids using mobile
phase port A and mobile phase port B.
Criteria
• "Retention time" coefficient of variation: 0.5 % max.
• "Peak area" coefficient of variation: 1.0 % max.
Before starting the test, rinse the flow line depending on the operating condition
of the instrument.
1 Instead of the column, connect the column inlet and outlet tubes to the PEEK
tubing using a coupling.
5 Put 1.0 mL of water in a sample vial, add a new septum, and close the cap.
System Guide "3.7 Setting a Sample"
6 Put 1.0 mL of sample in another sample vial, add a new septum, and close the
cap.
System Guide "3.7 Setting a Sample"
7 Start the temperature adjustment of the column oven and begin pumping for
the solvent delivery module.
Check that liquid is flowing from the outlet tubing of the detector and that there is no
fluid leaking from the connection part.
8 Monitor the baseline, and, if a stable baseline is obtained, conduct the zero
adjustment for the detector.
2
9 Inject 5 µL of water and check whether a peak is observed.
11 From the six analysis results, calculate the retention time of the peak and the
relative standard deviation (coefficient of variation) of the areas.
: Analysis count
: Mean
: Coefficient of variation
Using the ratio between the measured peak areas, this validation checks the degree of
retention for the autosampler sample.
CHECK CRITERIA
Carryover £ 0.010 %
1 Put 1 mL of sample in a sample vial, add a septum, and close the cap.
System Guide "3.7 Setting a Sample"
• Isocratic analysis
For the parameters other than above, configure the same settings as in Procedure 4 of
"Isocratic analysis" P.84 in "2.5.1 Analysis Reproducibility Test".
"Parameter setting values for the test" P.85
8 Calculate the ratio (%) of the peak area value of the sample to the peak area
obtained from the 250 mg/L caffeine solution sample.
Carryover = (Peak area of blank sample / Peak area of caffeine solution sample) x 100
Carryover £ 0.010
CHECK CRITERIA
Absorbance deviation of each concentration: ±5.0 % max.
For the parameters other than above, configure the same settings as in Procedure 4 of 2
"Isocratic analysis" P.84 in "2.5.1 Analysis Reproducibility Test".
1 Inject 35 µL.
2 If the height of the detection peak does not fall between 1.4 AU and 1.7 AU,
adjust the injection volume.
7 Analyze the 1st solvent to 5th solvent under the condition determined in
Procedure 6, and read the peak height.
The height can be found more easily if the waveform processing of LabSolutions is used.
LabSolutions Help
1 Write the sample concentrations [mg/L] in (1) to (5) in the table below.
2 Write the absorbance values (peak heights) [AU] in (A) to (E) in the table below.
3 Calculate the relative sensitivity for each concentration according to the equations
in the table below, and write in 1 to 5.
5 Calculate the deviation of each concentration using the following equation, and
record.
Deviation[%] = (Relative sensitivity - Standard relative sensitivity) / Standard relative
sensitivity x 100[%]
n Causes why the linearity does not meet the CHECK CRITERIA and corrective actions
The Mirror or Aperture Plate Has Conduct linearity recalibration or optical system
Deteriorated. maintenance. (Contact your Shimadzu representative.)
CHECK CRITERIA
Absorbance deviation of each concentration: ±5.0 % max.
For the parameters other than above, configure the same settings as in Procedure 4 of
"Isocratic analysis" P.84 in "2.5.1 Analysis Reproducibility Test".
1 Inject 35 µL.
2 If the height of the detection peak does not fall between 1.4 AU and 1.7 AU,
adjust the injection volume.
7 Analyze the 1st solvent to 5th solvent under the condition determined in
Procedure 6, and read the peak height.
The height can be found more easily if the waveform processing of LabSolutions is used.
LabSolutions Help
1 Write the sample concentrations [mg/L] in (1) to (5) in the table below.
2 Write the absorbance values (peak heights) [AU] in (A) to (E) in the table below.
3 Calculate the relative sensitivity for each concentration according to the equations 2
in the table below, and write in 1 to 5.
5 Calculate the deviation of each concentration using the following equation, and
record.
Deviation[%] = (Relative sensitivity - Standard relative sensitivity) / Standard relative
sensitivity x 100[%]
Absorbance
Sample
Value Relative Sensitivity Deviation
Item Concentration
(Peak Height) [AU / (mg/L)] [%]
[mg/L]
[AU]
1st
(1) (A) 1 = (A) / (1)
Solvent
2nd
(2) (B) 2 = (B) / (2)
Solvent
3rd
(3) (C) 3 = (C) / (3)
Solvent
4th
(4) (D) 4 = (D) / (4)
Solvent
5th
(5) (E) 5 = (E) / (5)
Solvent
n Causes why the linearity does not meet the CHECK CRITERIA and corrective actions
The Mirror or Aperture Plate Has Conduct linearity recalibration or optical system
Deteriorated. maintenance. (Contact your Shimadzu representative.)
2 Press [Maintenance].
The [Maintenance] screen is displayed.
3 Press (Log).
n Calibration overview
Any individual item of a unit can be calibrated. On the [Hardware Calibration] screen of
the [Maintenance] screen, select an item and run calibration while operating the screen.
A PDA detector system model has some items that can be calibrated by the accessory
PDA Utility. For details, refer to the Help section for the PDA Utility.
n Calibration items
Items that can be calibrated by the PDA Utility (PDA detector system model)
The PDA Utility calibrates the following items. For operation details, refer to the Help
section for the PDA Utility.
• Exposure time setting
• Wavelength calibration
This section explains how to display each item on the [Hardware Calibration] screen.
3
1 Press (Menu).
2 Press [Maintenance].
The [Maintenance] screen is displayed.
3 Press (Calibration).
3.2.2 Pump
The mobile phase (water) is pumped at 1 mL/min. Use a stopwatch to record the time
it takes to pump 5 mL. Based on the recorded time, the flow rate accuracy is measured.
After measurement, the flow rate is automatically calibrated based on the measurement
results.
2 Press the [Pump] tab and then press [Flow rate accuracy].
The [Flow rate accuracy] screen is displayed.
5 Press [Next].
7 Press [Start].
Purging starts and the screen showing the 3
purge progress is displayed.
9 Insert the end of the tube into the validated measuring cylinder or measuring
flask, and use a stopwatch to measure the time it takes to pump 5 mL.
"Flow rate accuracy test" P.32 step9
12 Press [Close].
The Confirmation screen closes.
13 Press [Finish].
Use water and caffeine solution to perform binary low-pressure gradient. Absorbance for
each set concentration is measured and the concentration accuracy is calculated.
After measurement, the gradient concentration is automatically calibrated based on the
measurement results.
Measurement conditions
Item Requirements
Set Flow Rate 1 mL/min
Preset Wavelength 272 nm
Column Set Temperature 40 °C
A/B, A/C, or A/D 100/0 % for 10 min
A/B, A/C, or A/D 90/10 % for 5 min
Gradient Program A/B, A/C, or A/D 50/50 % for 5 min
A/B, A/C, or A/D 10/90 % for 5 min
A/B, A/C, or A/D 0/100 % for 5 min
Select the desired ports to be used from the combinations A/B, A/C, or A/D. Set
a 10 mg/L caffeine solution for mobile phase port A, and set water for mobile
phase ports B, C, D, and the rinse solution port. (Fill with water those ports that
will not be tested.)
6 Press [Start].
Rinse starts.
7 Press [Next].
The results of system checks are displayed.
3
9 Press [Close].
The Confirmation screen closes.
10 Press [Exit].
3.2.3 Autosampler
Use a 1.5 mL sample vial containing sample (water), and measure the mass before and
after injection of 50 µL. Repeat this operation 10 times. Based on the difference in mass,
the injection volume accuracy is calculated.
After measurement, the injection volume is automatically calibrated based on the
measurement results.
3
1 Display the [Hardware Calibration] screen.
"3.2.1 Displaying the Hardware Calibration Items" P.103
2 Press the [Autosampler] tab and then press [Injection Volume accuracy].
The [Injection Volume accuracy] screen is displayed.
6 Press [Next].
11 Press [Close].
The Confirmation screen closes.
12 Press [Exit].
Use an external thermosensor and measure the temperature of the sample cooler. The
temperature accuracy of the sample cooler is calculated based on the measured
temperature. The set value of the sample cooler is "15 °C".
After measurement, the sample cooler temperature is automatically calibrated based on
the measurement results.
2 Press the [Autosampler] tab and then press [Cooler Temperature accuracy].
The [Cooler Temperature accuracy] screen is displayed.
4 Press [Next].
The sample cooler starts temperature
control at a set temperature of 25 °C.
8 Press [Close].
The Confirmation screen closes.
9 Press [Finish].
Select [1 Point] when only 1 oven temperature is set. Select [2 Points] when 2 or more
oven temperatures are set. Set any temperature for running calibration by using the 3
following procedure.
Set a calibration temperature. After temperature stabilization, use the external
thermosensor to measure the temperature of the column holder. The oven temperature
can be found from the measured temperature.
After measurement, the oven temperature is automatically calibrated based on the
measurement results.
To calibrate with 2 points, for the first point, set to 60 °C or below for the
calibration temperature, and for the second point, set to at least 20 °C higher.
If necessary, tape at
2 or more points to
ensure that the
external
thermosensor will
not fall off the
column holder.
5 Press [Next].
7 Press [Next].
10 Press [Close].
The Confirmation screen closes.
11 Press [Exit].
3
12 Check the temperature accuracy.
"Column oven set temperature
accuracy / Temperature control
accuracy test" P.50
The operating status of the gas sensor is checked, and the gas sensor sensitivity is
calibrated.
After measurement, the gas sensor operation is automatically calibrated based on the
measurement results.
Measurement conditions
Item Requirements
Oven Temperature 50 °C
4 Press [Next].
6 Press [Next].
The results of system checks are displayed.
8 Press [Close].
The Confirmation screen closes.
9 Press [Exit].
3.2.5 UV Detector
4 Press [Start].
Purging starts and the screen showing the
purge progress is displayed.
6 Press [Next].
The results of system checks are displayed.
8 Press [Close].
The Confirmation screen closes.
9 Press [Exit].
The absorbance is measured twice, once with the external standard filter not mounted
and once with the filter mounted. Based on the difference and the absolute absorbance
of the external standard filter, the absorbance accuracy of the detector is calculated.
After measurement, the absorbance accuracy is automatically calibrated based on the
measurement results.
5 Press [Next].
Absorbance (background absorbance) with
the filter not mounted is adjusted to "0"
and the [Filter Installing] screen is
displayed.
7 Press [Start].
With the optical filter to calibrate
absorbance (external standard filter)
mounted, measurement of the absorbance
starts.
8 Press [Next].
The results of system checks are displayed.
10 Press [Close].
The Confirmation screen closes.
11 Press [Exit].
"3.1 Exposure Time Setting" and "3.2 Wavelength Calibration" in the PDA Utility Help
The difference between the preset wavelength and the measured wavelength is
calibrated. Using the emission line of the D2 lamp (deuterium (D2) lamp) and the built-in
filter (holmium oxide filter) lambda max, the wavelength accuracy is checked.
After measurement, the wavelength accuracy is automatically calibrated based on the
measurement results.
Item Wavelength
241.5 nm
Holmium Oxide Filter Lambda Max
360.8 nm
486.0 nm
Emission Lines of the D2 Lamp
656.1 nm*
3
The above wavelengths assume that a 1.2 nm slit is used. When an 8 nm slit is
used, only the wavelength marked with * is checked. (The 1.2 nm and 8 nm slits
are automatically changed.)
4 Press [Start].
Purging starts and the screen showing the
purge progress is displayed.
6 Press [Next].
The results of system checks are displayed.
8 Press [Close].
The Confirmation screen closes.
3
9 Press [Exit].
The absorbance is measured twice, once with the external standard filter not mounted
and once with the filter mounted. Based on the difference and the absolute absorbance
of the external standard filter, the absorbance accuracy of the detector is calculated.
After measurement, the absorbance accuracy is automatically calibrated based on the
measurement results.
Wait for 90 minutes or more after turning on a lamp. Because absorbance is not
stabilized immediately after lamp lighting.
3
5 Press [Next].
Absorbance (background absorbance) with
the filter not mounted is adjusted to "0"
and the [Filter Installing] screen is
displayed.
7 Press [Start].
With the optical filter to calibrate
absorbance (external standard filter)
mounted, measurement of the absorbance
starts.
8 Press [Next].
The results of system checks are displayed.
10 Press [Close].
The Confirmation screen closes.
11 Press [Exit].
3
After the check, remove the
optical filter to calibrate
absorbance (external standard
filter) immediately. The filter
may be damaged.
The exposure time of the D2 lamp is set according to the slit widths (1.2 nm and 8 nm).
When the optional W lamp is connected, the exposure time and voltage are also set.
4 Press [Start].
Purging starts and the screen showing the
purge progress is displayed.
7 Press [Close].
The Confirmation screen closes.
3
8 Press [Exit].
When the leak sensor sensitivity is not optimal due to changes in the operating
environment, adjust the leak sensor sensitivity (threshold).
The leak sensor of the detectors, pump, autosampler (HPV: high-pressure valve), and
autosampler (LPV: low-pressure valve) can be adjusted.
2 Press the [Other] tab and then press [Leak sensor calibration].
The [Leak sensor calibration] screen is displayed.
UV/PDA detector
Pump
Autosampler (HPV)
Autosampler (LPV)
5 Press [Start].
The [Calibration] screen opens and
measurement starts.
Item Explanation
Dry Value Monitor value while dry
Wet Value Monitor value while wet
Threshold
Threshold before
(Before
measurement
Measurement)
New threshold calculated
Threshold
based on the measured
(New)
value
8 Press [Close].
The Confirmation screen closes.
9 Press [Close].
This section explains how to display each item on the [System Adjustment] screen.
1 Press (Menu).
2 Press [Maintenance].
The [Maintenance] screen is displayed.
3 Press (Calibration).
For each position where a plate is fitted onto a sample rack, adjust the position to which
the needle moves down by following the on-screen instructions.
• Place the plate type selected on the settings screen onto the sample rack. If
a different type is used, adjustment cannot be made properly.
• The stored rack position includes the combinations of the right/left plates, the
rack IDs, and the plate types. Adjust the rack positions for all plates to be set
on each sample rack. When the rack IDs or plate types are changed, adjust
the rack positions again.
Plate Type
Display Adjustment For 1 mL For 1.5 mL For 4 mL
No. Position 96 Hole 384 Hole
Sample Sample Sample
MTP/DWP MTP/DWP
Vial Vial Vial
Front Left
1 Corner of the 1 1 1 A01 A01
Front Plate
Front Right
2 Corner of the 73 46 22 H01 P01
Front Plate
Rear Left
3 Corner of the 12 9 7 A12 A24
Front Plate
Front Left
4 Corner of the 1 1 1 A01 01
Rear Plate
When adjusting the rack position with sample vial plates, insert the sample vials
into all positions. The rack position cannot be adjusted with only the plates.
ID.
3
5 Attach the rack teaching jig to the Z
mount.
1 Align the hole of the rack
teaching jig (B) with the threaded
screw hole (a) at the bottom on
the left side of the Z mount and
attach the cap/knob (A).
Do NOT attach the sample vial cap when adjusting the 4 mL vial plate position.
The cap may make contact with the teaching jig, which can cause errors. If an
error occurs, restart the instrument and readjust the position again.
8 Press [Next].
The Adjustment screen for [Position1] is displayed, and the needle moves to [Position1].
• Use [Vertical
movement] to drop the
tip of the needle to a
height that makes it
easy to check the
relative position
between the needle tip
and the left-right and
front-back positions of 3
the hole.
• Use [Parallel
movement] to align
the tip of the needle
with the center of the
hole.
2 Press [Next].
The next [Position] adjustment
screen is displayed, and the
needle moves near the next
position.
Direction Operation
(Left-Right
and
The needle moves in 0.1 mm increments.
Front-Back
Directions)
First needle drop: 4.0 mm. Second and subsequent drops: 0.2
(Down)
mm.
First needle drop: 4.0 mm. Second and subsequent drops: 1.0
(Down (Large))
mm.
• Press [Stop] to cancel the adjustment during the procedure. The adjustment is
finished without updating the needle position.
• [Vertical movement] is for checking the relative position with the hole by
bringing the tip of the needle closer to a well or a sample vial. This function
is not for deciding the needle stroke.
11 Press [OK].
The message "Remove the teaching jig." is
displayed.
13 Press [Close].
Check and adjust the position of the autosampler needle when it moves down to the
injection port of the high-pressure valve. After needle replacement, adjust if the position
has shifted.
3
1 Display the [System Adjustment] screen.
"3.3.1 Displaying the System Adjustment Items" P.152
4 Press [Next].
The Adjustment screen for the needle position is displayed.
To adjust the vertical position, set the tip of the needle at the same
height as the top of the hole (B) on the injection port.
2 Press [Next].
The Confirmation screen is
displayed.
(Left-Right
and
The needle moves in 0.1 mm increments.
Front-Back
Directions)
First needle drop: 4.6 mm. Second and subsequent drops: 0.1
(Down)
mm. 3
First needle drop: 4.6 mm. Second and subsequent drops: 1.0
(Down (Large))
mm.
Press [Stop] to cancel the adjustment during the procedure. The adjustment is
finished without updating the needle position.
1 Press [Start].
The sealing operation is
performed twice. Check that the
needle moves down to the
injection port properly.
7 Press [Start].
The sealing operation is repeated 50 times
to create a smooth seal surface.
B T
Balance 44 Temperature accuracy calibration 121
Baseline noise/drift test 64, 74
U
C UV Detector 94
Calibration 102
Column oven set temperature accuracy / V
Temperature control accuracy test 50 Validation 2
Cooler temperature accuracy calibration 117
W
E Wavelength accuracy / Lamp energy test 57, 68
Exposure time / W lamp voltage setting 143 Wavelength accuracy calibration 129, 137
F
Flow rate accuracy calibration 106
Flow rate accuracy test 32
G
Gas sensor test 54
Gas sensor operation calibration 126
Gradient concentration accuracy calibration 109
Gradient concentration accuracy test 38
H
Hardware Calibration 102
holmium oxide filter 57
I
Injection volume accuracy calibration 112
Injection volume accuracy test 44
Items that can be calibrated by the PDA Utility 103
L
Leak Sensor Calibration 146
Leak sensor test 78
O
Other Validations 83
P
PDA Detector 97
Performance Check 30
Pressure limiter test 41