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PLoS Pathog. 2011 Oct;7(10):e1002333. doi: 10.1371/journal.ppat.1002333. Epub 2011 Oct 27.

Herpesvirus telomerase RNA (vTR) with a mutated template sequence abrogates herpesvirus-
induced lymphomagenesis.
Kaufer BB1, Arndt S, Trapp S, Osterrieder N, Jarosinski KW.
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Abstract
Telomerase reverse transcriptase (TERT) and telomerase RNA (TR) represent the enzymatically active components of telomerase. In the
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complex, TR provides the template for the addition of telomeric repeats to telomeres, a protective structure at the end of linear chromosomes.
Herpesvirus telomerase RNA(vTR)-dependent
Human TR with a mutation in the template region has been previously shown to inhibit proliferation of cancer cells in vitro. In this report, we
lymphoma formation does not[PLoS
require
Pathog.
interaction
2010]
examined the effects of a mutation in the template of a virus encoded TR (vTR) on herpesvirus-induced tumorigenesis in vivo. For this
purpose, we used the oncogenic avian herpesvirus Marek's disease virus (MDV) as a natural virus-host model for lymphomagenesis. We A virus-encoded telomerase RNA promotes
generated recombinant MDV in which the vTR template sequence was mutated from AATCCCAATC to ATATATATAT (vAU5) by two-step malignant T cell lymphomagenesis.
[J Exp Med. 2006]
Red-mediated mutagenesis. Recombinant viruses harboring the template mutation replicated with kinetics comparable to parental and The RNA subunit of telomerase is encoded by
revertant viruses in vitro. However, mutation of the vTR template sequence completely abrogated virus-induced tumor formation in vivo, Marek's disease virus. [J Virol. 2003]
although the virus was able to undergo low-level lytic replication. To confirm that the absence of tumors was dependent on the presence of
Review Telomeres and Telomerase: Role in
mutant vTR in the telomerase complex, a second mutation was introduced in vAU5 that targeted the P6.1 stem loop, a conserved region
Marek's Disease Virus Pathogenesis,
[Viruses.
Integration
2017]
essential for vTR-TERT interaction. Absence of vTR-AU5 from the telomerase complex restored virus-induced lymphoma formation. To test if
the attenuated vAU5 could be used as an effective vaccine against MDV, we performed vaccination-challenge studies and determined that Review Marek's disease: Genetic regulation of
vaccination with vAU5 completely protected chickens from lethal challenge with highly virulent MDV. Taken together, our results demonstrate gallid herpesvirus 2 infection and latency.
[Vet J. 2015]
1) that mutation of the vTR template sequence can completely abrogate virus-induced tumorigenesis, likely by the inhibition of cancer cell
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proliferation, and 2) that this strategy could be used to generate novel vaccine candidates against virus-induced lymphoma.
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PMID: 22046133 PMCID: PMC3203187 DOI: 10.1371/journal.ppat.1002333

[Indexed for MEDLINE] Free PMC Article

Cited by 9 PubMed Central articles
Identification of the Receptor and Cellular
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Microbiol.
(MDV) 2017]
Images from this publication. See all images (8) Free text Epstein-Barr virus-encoded RNAs (EBERs)
complement the loss of Herpesvirus
[Sci
telomerase
Rep. 2018]

Review Telomeres and Telomerase: Role in

Marek's Disease Virus Pathogenesis,
[Viruses.
Integration
2017]

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