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BAB IV

HASIL DAN PEMBAHASAN

4.1 Hasil

4.1.1 Pembuatan Larutan Parasetamol 2000 ppm 250 ml

Ppm = ug/ml

2000 ppm = 2000 ug/ml

= 2000 x 250 ug/250ml

= 500.000 ug/ 250 ml

= 500 mg/250 ml

4.1.2 Kurva Kalibrasi

4.1.3 Perhitungan Konsentrasi


Waktu Absorbansi Pengenceran Konsentrasi
y=0.0447x+0.0881
0.194=0.0447x + 0.0881
10 0.194 5x
x= 1.202 ppm
Konsentrasi = 1.202 x 5 = 6.010 ppm
y=0.0447x+0.0881
0.2.38=0.0447x + 0.0881
20 0.238 5x
x= 1.701 ppm
Konsentrasi = 1.701 x 5=8.507 ppm
y=0.0447x+0.0881
30 0.205 5x
0.205=0.0447x + 0.0881
x= 1.3269 ppm
Konsentrasi = 1.3269 x 5 =6.6345 ppm
y=0.0447x+0.0881
0.174=0.0447x + 0.0881
40 0.174 5x
x= 0.975ppm
Konsentrasi = 0.9750 x 5 =4.875 ppm
y=0.0447x+0.0881
0.138=0.0447x + 0.0881
50 0.138 5x
x= 0.5664 ppm
Konsentrasi = 0.5664 x 5 =2.8320 ppm
y=0.0447x+0.0881
0.11=0.0447x + 0.0881
60 0.110 5x
x= 0.24858 ppm
Konsentrasi = 0.24858 x 5 =1.24291 ppm
y=0.0447x+0.0881
0.106=0.0447x + 0.0881
70 0.106 5x
x= 0.20318 ppm
Konsentrasi = 0.20318 x 5 = 1.101589 ppm

4.1.4 Data Konsentrasi Per Satuan Waktu


Waktu (menit) Konsentrasi (ppm) Ln C
10 6.010 1.793
20 8.507 2.141
30 6.634 1.892
40 4.875 1.584
50 2.832 1.041
60 1.243 0.217
70 1.016 0.0158

Kurva PCT peroral dalam satuan


Waktu
2.5

1.5
Kurva PCT peroral
1 dalam satuan Waktu

0.5

0
0 20 40 60 80
Kurva PCT peroral Tanpa menit 60
2.5

1.5 Kurva PCT peroral


Tanpa menit 60

0.5

0
0 20 40 60 80

4.1.5 Nilai Parameter


Jika di dapatkan dari kurva diatas sebuah persamaan
Laju Eliminasi → Ln Ct =3.156 – 0.043x
Nilai Parameter Teoritis Praktikum
C0 D0 X F/ vd Ln Ct = 3.156 – 0.043x
10 mg x 0.49/0.25 L Ln C0 =3.156 – 0.043(0)
=19.6 ppm` Ln C0 = 3.156
C0 = 23.4765 ppm
K eliminasi k = Cl / Vd
Ln Ct = 3.156 – 0.043x
k = 10 ml/menit /250 ml
K eliminasi =0.043/menit
k = 0,04 / menit
T1/2 eliminasi T1/2 → 0.693/k = 0.693/0.04 T1/2 → 0.693/k = 0.693/0.043
=17.3286 menit =16.1197 menit
Vd 250 ml Vd = DoXF / Co
Vd = 10 mg x0.49 / 23.4765 µg/ml
Vd = 0.209 L = 209 ml
Cl 10 ml/menit Cl = k x Vd
Cl = 0,043 / menit x 425 ml
Cl = 18.275 /menit
AUC AUC = Co / k AUC = Co / k
AUC = 19.6/ 0.04/menit AUC =23.475 µg/ml / 0,043/menit
AUC = 490 µg.menit/ml AUC =545.96 µg.menit/ml
F Tidak diketahui 𝐹 𝐾𝑎 𝐷0
Cp =𝑉𝑑 (𝐾𝑎−𝑘)(e-kt- e-kat)
𝐹 0.23 𝑥 10 𝑚𝑔
6.63 ppm =0.25 (0.23−0.043)(e-0.043x30-

e-0.23x30)
𝐹 2.3
6.63 ppm =0.04675(0.274)

F =0.49
B D0 x F/ vd Ln Ct = 3.156 – 0.043x
10 mg x 0.49/0.25 L Ln C0 =3.156 – 0.043(0)
=19.6 ppm` Ln C0 = 3.156
C0 = 23.4765 ppm
β k = Cl / Vd
Ln Ct = 3.156 – 0.043x
k = 10 ml/menit /250 ml
K eliminasi =0.043/menit
k = 0,04 / menit
α Tidak Diketahui 𝑙𝑛 𝐶1′−𝑙𝑛 𝐶2′
Ka = 𝑡2−𝑡1
3.156−1.7
Ka = 10−0

Ka = 0.23
Persamaan Tidak diketahui Cp = 23.4765 (e-0.043t – e-0.23t)