GREEN SYNTHESIS OF SILVER

NANOPARTICLES USING
PLANT EXTRACT

By
Abhishek Indurkar

1
 KIT,s College of Engineering, Kolhapur

Introduction
Silver Nanoparticles

• Nanotechnology is manipulation of matter on an atomic and molecular range.

• Size of nanoparticles = between 1 and 100 nanometer (1 nm = 10-9 m) in size.
• Silver nanoparticles show wide application in the field of nanomedicine.[1]
• Applications of nanotechnology
1. Nanomedicine
2. Nanoelectronics
3. Biomaterials energy production.[2]
Assignment for Research Methodology 2
 Green Synthesis of Silver Nanoparticles

• Silver nanoparticles can be synthesized by several methods.

• They can be divided into three broad categories:
Synthesis of Silver Nanoparticles[2]

1. Physical synthesis

2. Chemical synthesis

3.Biogenic synthesis

• Using plant extract[2]

• Using antibiotics[2]
• Using microorganisms[2]
3
• The biological synthesis of nanoparticles has provided a means for improved
techniques compared to the traditional methods that call for the use of harmful
reducing agents.
• Chemical and Physical methods for the synthesis are very costly, toxic and affects
the medical applications.
• Biogenic methods of silver nanoparticles synthesis are ecofriendly and produce
nanoparticle with precise shape and size.[2]

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[Ref 3]

5
 Main topic
Green Synthesis of Silver Nanoparticles
Using Plant Extract

• Biosynthesis of nanoparticles is considered a green technology, as it does not involve

any harmful chemicals.
• Several microorganisms, such as bacteria, fungi and yeasts have come up as Nano-
factories, synthesizing metal nanoparticles of Ag and Au.
• However, the use of plants for the fabrication of nanoparticles has drawn attention,
because of its rapid, economical, eco-friendly protocol, and it provides a single step
technique for the biosynthesis process.

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 Notes

• The synthesis of silver nanoparticles depend on phenolic content of different plant sources.
Ag+ reduction Ag0
• Different parameters like temperature, time, pH, etc. influence the size and shape of silver
nanoparticles.
• Antimicrobial activity of AgNPs can be studied using Well diffusion assay.
• Applications :
1. Effluent treatment process (for reducing the microbial load)[7]
2. Wound dressing

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 Methodology

1. Preparation of plant extract

2. Synthesis of silver nanoparticles
Ag+ ions were reduced by addition of plant extract to aqueous AgNO3 solution.

Reduction of the Ag+ ions was monitored by measuring the ultraviolet-visible spectrum
of the solution at regular intervals on a spectrophotometer operating at a resolution of 1
nm.

The effects of temperature on the rate of synthesis and size of the prepared silver
nanoparticles were studied by carrying out the reaction in a water bath at particular
temperature with reflux.

The concentration of AgNO3 solution was also varied

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 Scanning
Electron
Microscop
e

Fourier
UV
Transform
Visible
Infrared
Spectropho
Spectrosco
Analysis of tometer
py
silver
nanoparticles

Transmissi
on
X-ray
Electron
Diffraction
Microscop
e

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 Categorisation

Sr.parameters
The No. Source
like reaction timeTime Of Reaction
and size Size (nm)are considered.
of nanoparticles References
1. Garcinia morella 25 min 120.1 Borah D., et.al[5]
2. Citrus sinensis 1 hr 50-73 D.Subhashini [6]
3. Pomelo Fruit 24 hrs 2.5-5.7 D. Sarvamangala[8]
4. Tribulus terrestris 36 hrs 24.63 V. Gopinath[9]
5. Aloe vera 24 hrs 500 Hashoosh S.I,et.al[3]
6. Lemon Extract 24 hrs 30.5 Selvakumar P,et.al[10]
7. Banana peel extract 72 hrs 23.7 Ibrahim H.M.M [7]
8. Oak fruit bark 30 min 20-25 Veisi H.,et.al [11]
9. Carambola fruit extract 1 hr 18 Mane Gavade S J. [12]
10. Apple Extract 1 hr 30.25 Abidin Ali Z.,et.al [13]
11. Malus domestica 24 hrs 20 Roy k.,Et.Al [14]
Assignment for Research Methodology 10
 Detailed Outline
Synthesis of silver nanoparticles

Introduction
Slide # 1

Chemical Physical Biological

Narrowing topic
Slide # 2,3

Micro-organisms Antibiotics Plant extract

Literature Slide # 8,9,10

Parameters

Time Size

References
11
Slide # 5,6, 7.
 Detailed Outline

• Introduction
• Notes
• Out of different methods, biogenic methods of synthesis of silver nanoparticles were
selected.
• Green synthesis of silver nanoparticles using plant extract (mainly fruit extract) was
selected.
• Different parameters in the synthesis of silver antibiotic were studied. Reaction time
and size of nanoparticles are selected for detailed study.

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THANK YOU…

13
 References

1. Satoshi H. and Serpone N., (2013).“Microwave in nanoparticle synthesis”, first edition, Wiley-VCH
Verlag GmbH & Co. KGaA.
2. Rauwel P. (2015) “silver nanoparticles: synthesis, Properties and applications”, Hindawi Publishing
cotrpotratrion, Advances in material Science and engineering.
3. Priyanka Singh., (2016), “Biological Synthesis of Nanoparticles from Plants and Microorganisms”,
vol 34, p588–599.
4. Ibrahim H.M.M., (2015)“Green synthesis and characterization of silver nanoparticles using banana
peel extract and their antimicrobial activity against representative microorganisms”, Journal of
Radiation Research and Applied Sciences, p265-275. [IMF above 2]

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 Bibliography

1. Satoshi H. and Serpone N., (2013).“Microwave in nanoparticle synthesis”, first edition, Wiley-
VCH Verlag GmbH & Co. KGaA.
2. Rauwel P. et al., (2015) “silver nanoparticles: synthesis, Properties and applications”, Hindawi
Publishing cotrpotratrion, Advances in material Science and engineering.
3. Singh P. et al., (2016), “Biological Synthesis of Nanoparticles from Plants and
Microorganisms”, vol 34, p588–599.
4. Hashoosh S. I.,et.al, (2014)“Production of Ag nanoparticles Using Aloe vera Extract and its
Antimicrobial Activity”, Journal of Al-Nahrain University, Vol.17 (2), p165-171.
5. Borah D. & Yadav A., (2015) “A Novel ‘Green’ Synthesis of Antimicrobial Silver
Nanoparticles (AgNPs) by using Garcinia morella (Gaertn) Desr. Fruit Extract” Nanoscience
& Nanotechnology-Asia,5, p25-31.

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6. Subhashini D., (2015) “Antibacterial potency of nanosilver synthesized from the fruit
filtrate of citrus sinensis”, International Journal of Pharma and Bio Sciences, p396 – 403. [
IMF 5.12]
7. Ibrahim H.M.M., (2015)“Green synthesis and characterization of silver nanoparticles using
banana peel extract and their antimicrobial activity against representative microorganisms”,
Journal of Radiation Research and Applied Sciences, p265-275. [IMF 2.18]
8. D. Sarvamangala, (2013)“Biogenic Synthesis of AGNP’s Using Pomelo Fruit –
Characterization and Antimicrobial Activity against Gram +Ve and Gram –Ve Bacteria”,
International Journal of Pharmaceutical Sciences Review and Research, p30-35. [IMF 2.54]
9. Gopinath V. et al., (2012) “Biosynthesis of silver nanoparticles from Tribulus terrestris and
its antimicrobial activity: A novel biological approach”, Colloids and Surfaces B:
Biointerfaces, 69– 74 [IMF 2.18]
10.Selvakumar P. et al., (2016)“Green Synthesis and Antimicrobial Activity of
Monodispersed Silver Nanoparticles Synthesized Using Lemon Extract”, Synthesis and
Reactivity in Inorganic, Metal-Organic, and Nano-Metal Chemistry, 291–294

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11. Veisi H. et al., (2016) “Green synthesis and characterization of monodispersed silver
nanoparticles obtained using oak fruit bark extract and their antibacterial activity”,Applied
Organometallic Chemistry, vol 30, 387–391[IMF 2.248]
12. Mane Gavade S J., (2015) “Green synthesis of silver nanoparticles by using carambola fruit
extract and their antibacterial activity”, Advances in Natural Sciences: Nanoscience and
Nanotechnology, vol 6.
13.Zainal Abidin Ali, Rosiyah Yahya, Shamala Devi Sekaran, and R. Puteh, “Green Synthesis of
Silver Nanoparticles Using Apple Extract and Its Antibacterial Properties,” Advances in
Materials Science and Engineering, vol. 2016, Article ID 4102196, 6 pages, 2016.
doi:10.1155/2016/4102196.
14. Roy K., Sarkar C., & Ghosh C. (2014) “Green synthesis of silver nanoparticles using fruit
extract of malus domestica and study of its antimicrobial activity”, Vol. 9, p1137 – 11

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