LAB REPORT #5: Cardiovascular System

Biology I, Pre-Health Sciences – Durham College, Fall 2018

Name: Hannah Grant 100710803 /25 marks

EVALUATION NOTES

Read before submitting:

• The pre-lab quiz and lab report are worth 5% of

your final grade.

• This lab report is due by 11:59 pm on the due

date established by your instructor.

a. A report submitted after this due date is

considered late and will result in a grade of 0
unless a late submission is negotiated.
b. A negotiated late submission is a lab report
that has been submitted late for valid
reasons with the written permission of the
instructor. It is the responsibility of the
student to request permission to negotiate a
late submission no later than 48 hours prior
to the due date. Late submissions are
penalized 10% per day up to a max of 3
days (including weekends). After that a zero
is assigned.

• This lab report should be submitted online to the

DC Connect Assignments folder.

a. The report should be submitted using a fillable PDF available on DC Connect.

An instructional video is posted on how to use a fillable PDF (or click here).
Unless otherwise directed, submissions made by other means (E.g. email or
hard copy) will not be accepted and will result in a grade of 0.
b. Computer malfunctions or technical issues are not accepted as excuses for
late or missing reports. TIP: Submit your report early so that you have time to
resolve any potential technical issues prior to the deadline.

• While students work in pairs to complete labs, each student must submit their own
original work.

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PART A – BLOOD TESTS (9 marks)

1. (3 × 0.5 marks) Submit Table 5.1 (p. 67 of the lab manual). To do this, place a “Y”
or “N” in the boxes below depending on if agglutination (clumping) occurred for each
antibody. Then use this information to deduce the ABO blood type for each patient.

Patient Y/N – Did agglutination occur? Blood

Blood Anti-A antibody Anti-B antibody Anti-Rh antibody Type

Carrie Y N Y A+

Tina Y Y Y AB+

Tricia N Y N B-

2. (3 x 0.5 marks) Take a photo of the picture that you drew of Slide K using the 40X
objective (p. 68 of the lab manual). For full marks, make sure that your drawing has
the following structures labeled for one of the cells present in the image: red blood
cell, platelet, white blood cell

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3. (2 x 1 mark) Classify Tricia’s blood as either “healthy” or “sickle-cell anemia.’
Justify your answer using 2 pieces of evidence collected from Slides I/J/K on p. 68
of the lab manual.

Choice: Sickle-Cell
Healthy or Sickle Cell Anemia
Reason 1: Tricia's blood had a much lower red blood cell count then normal
_____________________________________________________
blood
Tricia's red blood cells have a long, pointy sickle, non-oval shape
Reason 2: _____________________________________________________

4. Answer the questions below using the information collected from the “complete
blood count” (CBC) on p. 69 of the lab manual.
a. (1 mark) Some medical articles suggest that amphetamine overdose is linked
to leukocytosis. This leads to low white blood cell counts. Is there any
evidence of this in the CBC reports for Carrie, Tina, and Tricia?
Yes or NO
Select: YES
All the girls' blood had a lower white blood cell count than
Evidence: ___________________________________________________
normal, due to all of them also having a higher amphetamine
___________________________________________________
dose than normal. (Tricia had a higher wbc because of her
sickle cell anemia)
b. (1 mark) Recall that Tricia may have a pre-existing medical condition known
as sickle cell anemia. Sickle cell anemia often leads to an increase in white
blood cell and platelet counts while decreasing red blood cells counts, red
blood cell size, and hemoglobin. Is there any evidence from the CBC results to
suggest that this is in fact the case?
Yes or NO
Select: YES
Tricia's blood cell count did prove she has sickle cell anemia
Evidence: ___________________________________________________
because her wbc was higher than normal (13>11), her rbc was
___________________________________________________
lower than normal (3.5<4.0), and her platelet count was higher
than normal (450>400).
c. (2 marks) Amphetamine levels between 0.02 and 0.05 mg/L are considered
to be appropriate for therapeutic or prescribed use (e.g. for people with
ADHD). Levels greater than 2.5 mg/L can be toxic and possibly fatal.
According to the blood work in the “medical folder,” amphetamine is present in
all 3 patients; however, the severity of the overdose appears to be different.
Speculate at least 2 reasons why Carrie, Tina and Tricia could have different
levels of amphetamines in their blood despite taking the same pills.

Reason 1: ____________________________________________________

____________________________________________________

Reason 2: ____________________________________________________

____________________________________________________

3
PART B – TREATMENT (16 marks)

5. (3 × 0.5 marks) Compare the IV fluid that you made (p. 70-71 of the lab manual) to
blood plasma in real blood. In the spaces below, list 3 ingredients that are present
in real blood plasma that were not present in the IV fluid that you made.
albumin
___________________ fibrinogen
___________________ globulin
___________________

6. (3 marks) Complete the chart below using the information collected in Table 5.1 (p.
72 of the lab manual).

Y/N – Is Explain IN DETAIL why this transfusion is

Recipient Donor this safe or unsafe. Terms to include:
transfusion antigen, antibody, agglutination, red blood
safe? cell, plasma, donor, recipient
N The donor's A+ blood has A antigens and
Rh antigens on the surface on their red
blood cells, and B antibodies in the plasma.
Tricia: The recipient's B- blood has B antigens on
Type A+ the surface and A antibodies in the plasma.
B-
____ The B antigens in the recipients blood with
"link" with the B antibodies in the donor
blood and will cause agglutination.

Y O- is a universal donor. Type O blood has

neither A or B antigens on the surface of
the red blood cells, but has both A and B
Tricia: antibodies in the plasma. The recipient's B-
Type O- blood has B antigens on the surface and A
B-
____ antibodies in the plasma. Since there are no
antigens on the O blood, they will not "link"
with the A antibodies in the recipient blood,
causing no agglutination.

7. (6 x 0.5 marks) Match the heart anatomy in the chart below to the sticker label
present on the lab model (p. 73 of the lab manual).

Anatomy Sticker Label

aorta n
atrium – left g
valve – bicuspid atrioventricular j
valve – aortic semi-lunar k
vena cava c
ventricle – right b

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8. (10 x 0.5 marks) Use the information collected from the echocardiogram (p. 73 of the
lab manual), to track the pathway of blood through the heart. Start with the vena
cava and finish with the aorta. Remember to be as specific as possible.

BODY > Vena cava right atrium

> ______________ tricuspid valve
> ______________
right ventricle
>______________ pulmonary semi-lunar
> ______________ pulmonary
valve artery > LUNGS-left/right
> ______________
pulmonary vein
>______________ left atrium
> ______________ mitral valve
> ______________ left ventricle
> ______________
aortic semi-lunar valve
>______________ > aorta > BODY

9. (3 x 0.5 marks) Take a photo of the picture that you drew of Slide N using the 40X
objective (p. 74 of the lab manual). For full marks, make sure that your drawing has
the following characteristics labeled for one of the heart cells present in the image:
long tubular cells, branching, 1-3 nuclei per cell

10. (2 x 1 mark) Classify Tricia’s blood as either “healthy” or “heart attack.’ Justify your
answer using 2 pieces of evidence collected from Slides L/M/N on p. 74 of the lab
manual.

Choice: Healthy or Heart Attack

Tricia's heart tissue was long and branched like healthy a heart is
Reason 1: ________________________________________________________
supposed to be.
Tricia's heart tissue was rich and dense with cells, compared to the
Reason 2: ________________________________________________________
very stretched out, spacious cells of slide M which contained the
tissue affected by a heart attack