Ysi 7100

Op er a tions Ma nua l
YSI 7100 MBS

Multiparameter
Bioanalytical
System
www.ysil ifesciences.com
Ta b le of Con ten ts
1 Intr od uction .......................................................................................... 1- 1
1.1 Description................................................................................................................. 1-1
1.2 Capabilities ................................................................................................................ 1-2
1.3 User Features ............................................................................................................. 1-2
1.4 Major Components..................................................................................................... 1-3
1.5 Controls...................................................................................................................... 1-5
1.5.1 Display/Touch Panel............................................................................................... 1-5
1.5.2 Rear Panel ............................................................................................................... 1-6
2 Menu Flowcha r t .................................................................................... 2- 1
3 Getting Sta r ted .................................................................................... 3- 1
3.1 Unpacking .................................................................................................................. 3-1
3.2 Notes on Safety .......................................................................................................... 3-2
3.3 Warranty Card............................................................................................................ 3-2
3.4 What You Need.......................................................................................................... 3-3
3.5 Basic Setup................................................................................................................. 3-3
3.5.1 Connect Bar Code Scanner ..................................................................................... 3-4
3.5.2 Connect AC Power ................................................................................................. 3-4
3.5.3 Calibrate Sipper ...................................................................................................... 3-5
3.5.4 Setup Instrument Parameters ................................................................................ 3-11
3.5.5 Install Printer Paper............................................................................................... 3-30
3.5.6 Prepare and Install Buffer Solution(s) .................................................................. 3-31
3.5.7 Install Calibrator Solution(s)................................................................................. 3-32
3.5.8 Install Membranes on Enzyme Probes.................................................................. 3-34
3.5.9 Install Ion Selective Electrodes............................................................................. 3-35
3.5.10 Setup Bottles ....................................................................................................... 3-36
3.5.11 Prime the Fluid System....................................................................................... 3-45
3.5.12 Check Probe Currents ......................................................................................... 3-52
3.5.13 Calibrate.............................................................................................................. 3-54
3.5.14 Perform Daily Operational Checks..................................................................... 3-58
3.5.15 Sample................................................................................................................. 3-58
4 System Config ur a tion a n d Op er ation .................................................. 4- 1
4.1 Setup Menu ................................................................................................................ 4-1
4.1.1 System..................................................................................................................... 4-1
4.1.2 Clock ....................................................................................................................... 4-3
4.1.3 Sequence ................................................................................................................. 4-4
4.1.4 Sensor.................................................................................................................... 4-13
4.1.5 Scheduler............................................................................................................... 4-21
4.1.6 Ports ...................................................................................................................... 4-23
4.2 Service Menu ........................................................................................................... 4-25
i
4.2.1 Calibrate................................................................................................................ 4-25
4.2.2 Bottle..................................................................................................................... 4-29
4.2.3 Stir Bar .................................................................................................................. 4-38
4.2.4 Sipper .................................................................................................................... 4-40
4.2.5 Touch Panel .......................................................................................................... 4-45
4.2.6 Standby ................................................................................................................. 4-48
4.2.7 Software ................................................................................................................ 4-48
4.2.8 Remote .................................................................................................................. 4-49
4.2.9 Normal .................................................................................................................. 4-49
4.3 Diagnostics Menu .................................................................................................... 4-50
4.3.1 Probe ..................................................................................................................... 4-50
4.3.2 Fluid Pumps .......................................................................................................... 4-53
4.3.3 Stir Bars ................................................................................................................ 4-55
4.3.4 Sipper Pump.......................................................................................................... 4-55
4.3.5 Sipper .................................................................................................................... 4-58
4.3.6 Serial ..................................................................................................................... 4-60
4.3.7 Turntable............................................................................................................... 4-61
4.4 Data .......................................................................................................................... 4-62
4.4.1 Reports .................................................................................................................. 4-62
4.4.2 Export.................................................................................................................... 4-64
4.4.3 Manage.................................................................................................................. 4-67
4.5 Help Menu ............................................................................................................... 4-72
4.5.1 About..................................................................................................................... 4-73
4.6 Main Display............................................................................................................ 4-73
4.6.1 Start ....................................................................................................................... 4-74
4.6.2 Pause ..................................................................................................................... 4-75
4.6.3 Stop ....................................................................................................................... 4-75
4.6.4 Manual Sample ..................................................................................................... 4-76
4.6.5 Add Sample........................................................................................................... 4-79
5 Ch em istr y Setup ................................................................................... 5- 1
5.1 Principle ..................................................................................................................... 5-1
5.1.1 Enzyme Sensor........................................................................................................ 5-1
5.1.2 Ion Selective Electrode ........................................................................................... 5-2
5.2 Sample Preparation .................................................................................................... 5-2
5.3 Measurement Parameter Setup Information .............................................................. 5-3
5.4 D-Glucose (Dextrose) ................................................................................................ 5-5
5.5 L-Lactate .................................................................................................................... 5-6
5.6 Sucrose....................................................................................................................... 5-7
5.7 Ethanol ....................................................................................................................... 5-8
5.8 L-Glutamate (L-Glutamic Acid) ................................................................................ 5-9
5.9 L-Glutamine............................................................................................................. 5-10
5.10 Methanol .................................................................................................................. 5-11
ii
5.11 Galactose.................................................................................................................. 5-12
5.12 Xylose ...................................................................................................................... 5-13
5.13 Simultaneous Glucose and L-Lactate ...................................................................... 5-14
5.14 Simultaneous Glucose and Sucrose ......................................................................... 5-15
5.15 Simultaneous L-Glutamate and L-Glutamine.......................................................... 5-16
5.16 Simultaneous Ammonium and Potassium ............................................................... 5-17
5.17 Simultaneous Glucose and Xylose........................................................................... 5-18
6 Outp ut .................................................................................................. 6- 1
6.1 RS232 Serial Port....................................................................................................... 6-1
6.2 RS485 Serial Port....................................................................................................... 6-1
7 Stor a g e ................................................................................................. 7- 1
7.1 Instrument Storage ..................................................................................................... 7-1
7.2 Enzyme Membrane Storage....................................................................................... 7-1
7.3 ISE Storage ................................................................................................................ 7-1
8 Op er a tion a l Ch eck s a n d Ma in tena n ce ................................................ 8- 1
8.1 Daily Operational Checks .......................................................................................... 8-1
8.1.1 Enzyme Membrane Integrity Test .......................................................................... 8-1
8.1.2 Linearity Test .......................................................................................................... 8-2
8.1.3 Enzyme Membrane Replacement ........................................................................... 8-2
8.1.4 ISE Replacement..................................................................................................... 8-2
8.2 Daily Maintenance..................................................................................................... 8-3
8.3 Monthly Maintenance ................................................................................................ 8-4
8.3.1 Calibration Pumping System Maintenance............................................................. 8-4
8.4 Preventive Maintenance – 6 months or 1000 Hours.................................................. 8-6
8.4.1 Sensor Module Cleaning......................................................................................... 8-6
8.4.2 Enzyme Probe Cleaning.......................................................................................... 8-7
8.4.3 ISE Cleaning ........................................................................................................... 8-8
8.4.4 Tubing Replacement ............................................................................................... 8-8
8.4.5 Sipper pump Seal Replacement ............................................................................ 8-12
8.4.6 Calibrate Sipper .................................................................................................... 8-14
8.4.7 Prime Fluid System............................................................................................... 8-14
8.4.8 Install Membranes................................................................................................. 8-14
8.5 Preventive Maintenance – 12 Months or 2000 Hours ............................................. 8-14
8.5.1 Waste Chamber Cleaning ..................................................................................... 8-14
8.5.2 Sensor Module Cleaning....................................................................................... 8-15
8.5.3 Clean Enzyme Probes ........................................................................................... 8-15
8.5.4 Calibrator Tubing.................................................................................................. 8-15
8.5.5 Buffer Pump Tubing ............................................................................................. 8-15
8.5.6 Buffer Bottle Tubing............................................................................................. 8-15
iii
8.5.7 Valve Tubing ........................................................................................................ 8-15
8.5.8 Install Waste Chambers ........................................................................................ 8-16
8.5.9 Waste Tubing ........................................................................................................ 8-16
8.5.10 Install Sensor Modules........................................................................................ 8-16
8.5.11 Sipper Pump Seal Replacement .......................................................................... 8-16
8.5.12 Sipper Replacement ............................................................................................ 8-17
8.5.13 Sipper Mechanism Lubrication........................................................................... 8-18
8.5.14 Calibrate Sipper .................................................................................................. 8-18
8.5.15 Prime Fluid System............................................................................................. 8-18
8.5.16 Install Enzyme Membranes................................................................................. 8-18
8.6 Sipper Mechanical Alignment ................................................................................. 8-19
8.7 Fuse Replacement .................................................................................................... 8-20
9 Tr oub lesh ootin g ................................................................................... 9- 1
9.1 Printout Information................................................................................................... 9-2
9.2 Troubleshooting Chart ............................................................................................... 9-6
9.3 Bar Code Scanner .................................................................................................... 9-13
10 Pr incip les of Op er a tion ...................................................................... 10- 1
10.1 Enzyme Sensor Technology .................................................................................... 10-1
10.2 Measurement Methodology ..................................................................................... 10-2
10.3 Baseline Stability ..................................................................................................... 10-3
10.4 Calibration................................................................................................................ 10-3
10.5 Linearity................................................................................................................... 10-3
10.6 Temperature Compensation ..................................................................................... 10-4
10.7 Level Sensing........................................................................................................... 10-4
11 Accessor ies a nd Rep la cem ent Pa r ts .................................................. 11- 1
12 Ap p en d ix A - Sp ecifica tions ............................................................... 12- 1
12.1 7100 General Specifications .................................................................................... 12-1
12.2 Chemistry Performance Specifications.................................................................... 12-2
12.3 7110 Specifications.................................................................................................. 12-6
13 Ap p end ix B – Con cen tr a tion Unit Conver sion .................................... 13- 1
14 Ap p en d ix C - Wa r r a nty a nd Rep a ir ................................................... 14- 1
15 Ap p end ix D - Clea n in g , Disinfecting a n d Decon ta m in a tion ............. 15- 1
16 Ap p en d ix E - Req uir ed N otice............................................................ 16- 1
17 Ap p end ix F - Lin e Power Cor d a n d Plug Wir in g ................................ 17- 1
18 Ap p end ix G - CE Com p lia nce ............................................................. 18- 1
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1 Intr oduction
1.1 Descrip tion
The YSI 7100 MBS (Multiparameter Bioanalytical System) is a laboratory instrument intended
for use in research, food-processing and bioprocessing applications. THE YSI 7100 MBS IS
NOT FOR HUMAN MEDICAL DIAGNOSTIC USE OR FOR HUMAN
PERFORMANCE EVALUATION.
The 7100 MBS can be set up to measure up to 6 different analytes in a sample. The total number
of analytes depends on the number of sensor modules installed (two analytes per module) and the
number of different buffers required (up to three buffers). Up to three modules can be installed.
Available analytes for the 7100 MBS are listed below. Additional analytes are currently under
development. For a current listing, please contact YSI Life Sciences Customer Service (800 659-
8895 or 937 767-7241 extension 2) or visit the YSI web site at www.ysilifesciences.com.
D-Glucose (Dextrose) Potassium
L-Lactate Sucrose
L-Glutamate Ethanol
L-Glutamine Methanol
Ammonium Galactose 1
Xylose
Flexibility is an important feature of the product. Within practical limits a large number of
measurement parameters can be selected to fit your application. In addition, the default
parameters of many system functions can be changed to fit your needs.
Selectable sample sizes allow you to extend the dynamic range of the analyzer and improve
precision and accuracy. The aspirated sample size can be specified from a minimum of 10
microliters to a maximum of 50 microliters.
The 7100 MBS is equipped with automatic self-calibration. However, within limits, the
frequency of autocalibration can be programmed to meet your needs.
If instrument calibration is desired upon command only, the self-calibration feature can be
disabled and calibration cycles can be initiated manually via the touch panel.
YSI provides a full line of reagents to support the most common chemistries. Reagent bottles are
bar coded, allowing the 7100 MBS, with optional 7181 Bar Code Scanner, to read which
reagents are installed, expiration date and working life information.
YSI offers the 7110 Turntable for batch sampling of up to 30 vials. The 7110 mounts directly to
the 7100 MBS and batch sampling results are displayed, stored in memory and printed by the
7100.
The 7100 MBS has been designed for future additions to allow even greater versatility related to
sample handling and the measurement of additional analytes. Therefore, as accessories become
available or new chemistries are developed you have a basic instrument upon which to build.
1
YSI does not currently offer calibration standards for this analyte.
1-1
1.2 Ca p a b ilities
• True multiparameter measurement and display
• Modular Design
Expansion for additional sensor modules
• 3½” Floppy Disk Drive

Export sample and diagnostic data
Upgrade software
• RS232 and RS485 serial ports
Remote data logging
Remote control capability
• Field software updates
Via floppy disk drive
• AC line power
100–120/200–240 VAC (±10%), 50–60 Hz (±5%)
1.3 User Fea tur es

• Auto Calibration
• Backlit graphic 320x240 LCD
• Touch Panel menu system
• Audio feedback
• Real time clock
• 4-digit year
1-2
1.4 Ma jor Com p onen ts
The major components of the 7100 MBS are shown in Figure 1.1 and Figure 1.2.
Calibrator and Printer

Buffer Bottles
(under cover)
Display Contrast
Display/
Buttons
Touch Panel
3½″ Disk Drive

Pumps
Sipper
Test Tube Holders
Sensor Module 1
(optional enzyme
or ISE)
Sensor Module 3
Sensor Module 2
(enzyme)
(optional enzyme)
Waste Bottle
Figure 1.1
The Buffer Pump draws buffer from the buffer bottles, pumps it through the Sipper Pump and
the Sipper, and flushes the Sensor Modules.
The Calibrator Pumps draw the appropriate standard solution from the Calibrator Bottles and
fill the Calibrator Wells.
The Sipper pump retracts its piston to draw standard from the Calibrator Wells or sample from
the tube holders. It extends its piston to dispense standard or sample into each sensor module.
The Sipper is raised or lowered by one motor, and moved horizontally to its various positions by
another motor. The positions are: Calibrator Wells, Sensor Modules, Test Tube Holders and
Manual Sample. The Sipper senses fluid level to control immersion depth and detect errors.
The Stir Bars are plastic encapsulated magnets. They are activated by motors housed below the
sensor modules. They provide thorough mixing inside each sensor module.
1-3
The Buffer, Waste and Calibrator Bottles are conveniently located for maintenance. Fluid
levels are monitored by sensors. Operation is automatically halted when the Buffer or Calibrator
Bottles are empty, or when the Waste bottle is full.
Front Cover
(open) Buffer Valves
Calibrator
Pumps
Sipper Pump
Buffer Pump
Figure 1.2
The Sensor modules are made of clear acrylic plastic. Black holders for the sensor probes are
screwed to either side of each module. The immobilized enzyme membranes on the sensor
probes are mounted on O-rings which act as fluid seals. If an ISE module is installed, an O-ring
is positioned inside a sleeve on the end of the ISE. A reference or auxiliary electrode is housed in
the temperature probe and positioned at the back of each Sensor Module. A small O-ring slips
1-4
over each temperature probe/electrode to provide the seal. A Calibrator Well is located near each
Sensor Module entry port.
The Display is a graphical 320 x 240 liquid crystal display covered by a touch panel. Buttons on
the right side of the instrument adjust the display contrast.
The Printer provides a hard copy record of sample results, calibration information, and other
information. It uses 2 1/4 inch (56 mm) wide thermal paper.
The RS232 and RS485 serial ports are used to interface with host computers or other laboratory
instruments (such as the Groton Biosystems ARS-100).
The Keyboard port connects to the optional YSI 7181 Bar Code Scanner.
1.5 Con tr ols
1.5.1 Display/Touch Panel

Use the contrast buttons on the right side of the 7100 MBS to adjust the display contrast (see
Figure 1.1). A touch panel covers the display. Simply touch it with your finger, or the supplied
stylus, to select menus and functions. The Main screen is shown below in Figure 1.3. At the top
are buttons to select a sequence, start, pause or stop a sequence, or run a manual sample. The
Menu button and instrument status are at the bottom. In the center of the display, the first column
shows the chemistries and units that are assigned to each probe, while the remaining columns
display sample results.
Stop Button
Pause Button
Manual Sample
Button
Start Button
Add Sample
Button
Sequence Name
Sample ID
Sample Date/Time
Probe Chemistries and Sample Results

Concentration Units
Scroll Buttons
Menu Button Instrument Status
Figure 1.3
1-5
1.5.2 Rear Panel
The rear panel contains the main power switch, power receptacle, RS232 and RS485 serial ports,
a keyboard port, an expansion port and the fuse holder as shown below.
Fuse Power
Holder RS232 Port RS485
Receptacle
Port
100-120V~
Power 200-240V~
RS232 RS485
50/60Hz, 120W
Switch 250V 2A T
Keyboard
Port
Figure 1.4
Power Switch
The main power switch is an on/off toggle switch (0-off and I-on) located on the back of the
instrument.
Power Receptacle
One end of the power cord (supplied) plugs into this receptacle, while the other end plugs into a
properly grounded electrical outlet. The instrument will automatically adjust the voltage as
needed (see 12.1 7100 General Specifications).
RS232 Port
The RS232 connection is a standard DB9F connector. This connector allows the 7100 MBS to be
connected directly to the serial port of a computer using a null modem cable (see 11Accessories
and Replacement Parts).
RS485 Port
The RS485 connection is a standard DB9F connector. See 6.2 RS485 Serial Port for details.
Keyboard Port (Bar Code Scanner)

The keyboard port is a 6-Pin Mini DIN connector. Connect the optional YSI 7181 Bar
Code Scanner to greatly improve data entry when entering calibrator numbers.
Fuse Holder
The fuse holder houses the power line fuse and opens up for fuse replacement. See 8.7 Fuse
Replacement for more details.
1-6
2 Menu Flowcha r t
The software flow chart for the 7100 MBS is shown below in Figure 2.1. The main screen has
five buttons that control sampling and a menu button (shown at the top of the flowchart).
Touching the menu button brings up the selections shown below.
Start Pause Stop Menu Manual Sample Add Sample
Setup Service Diagnostics Data Help
Sequence Standby Probe Reports About
Calibrate
Tube Holders Sample Setup
TurnTable Flush
Bottle Export
Replicates Calibrator A
Calibrator B
Sensor Clear Bubble
General Type
Fluid Pumps Format
General Prime Data Range
Initialize
Detail Cal Manage
Chemistry
Buffer
End Point
Valves
Units Reagent Number Type
Enabled Lot Number Stir Bars Action
Sample Custom Store Properties
Detect Expiration
Volume Module 1
Stir Bar Module 2
Auto-Cal
Module 3
Sipper
Time Sipper Pump
Samples
Temperature Shift Module Position
Cal Shift Test Tube Position Home
Sample Error Liquid Detection Bubble
Calibrate Exercise
Touch Panel
Sipper
Location
Concentration Test
Calibrate Up, Down, Left, Right, Home
System Exercise, Vertical, Horizontal
Software
Serial
Sample Report
Remote
Calibration Report Turntable
Auto-Standby
Serial Number Normal
Waste Level Detection Exercise
Go To
Scheduler Increment
Home
Clock
Ports
Figure 2.1
2-1
2-2
3 Getting Sta r ted
3.1 Un p a ck ing
When you unpack your new YSI 7100 MBS for the first time, check the packing list to make
sure you have received everything listed. Note that reagents for the 7100 MBS are not packaged
in the same carton as the instrument. If there is anything missing or damaged, call the dealer
from whom you purchased the 7100 MBS. If you do not know which of our authorized dealers
sold the system to you, call YSI Life Sciences Customer Service at 800 659-8895 or 937 767-
7241, and we'll be happy to help you.
Remove Packing Material

After removing the 7100 from the shipping box, open the front cover (see Figure 1.2 on page
1-4).
Open the slot in the front of the cardboard packing material holding the sipper arm. Carefully
rotate the sipper arm and remove it from the packing material. Be careful not to pull on the
blue wire under the sipper arm. Position the sipper arm over module number 3.
1. Open slot, then rotate

and remove sipper
arm from slot
2. Remove packing
material
Slide the packing material out the side of the instrument.
3-1
3.2 N otes on Sa fety
DO NOT PLUG THE INSTRUMENT IN AT THIS TIME. You should apply power only
when directed to do so in the setup instructions.
NOTES ON SAFETY (Electrical precautions)
1. Use ONLY the line power cord supplied with the instrument. When directed to, connect the
plug to a matching three-pronged wall receptacle.
2. Use ONLY fuses of the type supplied (see 8.7 Fuse Replacement). Replacement power cords
and fuses can be obtained from YSI, or your Dealer Representative (see Appendix C -
Warranty and Repair and Appendix F - Line Power Cord and Plug Wiring.
3. Do NOT use an extension cord without protective grounding.
4. Do NOT remove front or rear cover. There are no user serviceable parts inside.
5. Repairs are to be performed only by trained and approved personnel.
6. This instrument must be connected to a protectively grounded (earthed) outlet.
7. The following notice is provided in compliance with IEC1010 Part 1 1990.
See Appendix F - Line Power Cord and Plug Wiring for mains plug wiring and fusing
instructions.
8. If the equipment is used in a manner not specified by YSI, the protection provided by the
equipment may be impaired.
WARNING: For RS232 or RS485 connection, refer to sections 6.1 RS232 Serial and 6.2
RS485 Serial . Equipment should be EN 61010 or EN 60950 approved only.
9. The mains (power) switch is for functional purposes ONLY. To disconnect the instrument
from the mains supply, unplug the mains power cord from the back of the instrument.
3.3 Wa rr a n ty Car d
Please complete the Warranty Card and return it to YSI. This will record your purchase of this
instrument in our computer system. Once your purchase is recorded, you will receive prompt,
efficient service in the event any part of your YSI 7100 MBS should ever need repair.
3-2
3.4 Wha t You N eed
Several things are needed in order to analyze samples using the YSI 7100 MBS. The following
list shows the basic items required.
• YSI 7100 MBS Instrument (with AC Power Cord)
• Optional Bar Code Scanner
• Buffer(s)
• Calibrator Standard(s)
• Linearity Standard(s)
• Membrane(s)
• ISE probes (7100-04A and 7100-06A only)
• Printer Paper
NOTE: See 11Accessories and Replacement Parts for a list of reagents and membranes.
3.5 Ba sic Setup

The following list describes the basic steps necessary for sampling with the 7100 MBS. See the
following sections for details of each step.
1. Connect Bar Code Scanner (optional)
2. Connect AC Power
3. Calibrate Sipper
4. Setup Instrument Parameters
5. Install Printer Paper
6. Prepare and Install Buffer solution(s)
7. Install Calibrator Solution(s)
8. Install Membrane(s)
9. Install ISE Probes (7100-04A and 7100-06A only)
10. Setup Bottles
11. Prime Fluid System
12. Check Probe Currents
13. Calibrate
14. Perform Daily Operational Checks
15. Sample
For example purposes, we will demonstrate how to set up a 3 module YSI 7100 MBS (1 ISE
module and 2 enzyme modules) to measure ammonium, potassium, glucose, lactate, glutamate
and glutamine.
3-3
3.5.1 Connect Bar Code Scanner
Use the optional YSI 7181 Bar Code Scanner to greatly improve data entry when entering
reagent numbers. The 7181 has a 6-Pin Mini DIN connector that plugs into the keyboard port in
the rear of the 7100MBS (see 1.5.2 Rear Panel for the location of the port).
Connect the bar code scanner as follows:
1. Make sure that the 7100 power switch is off.
2. With the arrow on the connector facing up, plug the bar code scanner into the Keypad
connector on the rear of the 7100.
WARNING: Do not look directly into the bar code scanner.
NOTE: If the 7181 Bar Code Scanner fails to scan properly, reprogram it as described in 9.3 Bar
Code Scanner.
3.5.2 Connect AC Power

Plug the power cord (included with the 7100 MBS) into the power receptacle on the back of the
instrument, then into a properly grounded electrical outlet. The instrument will automatically
adjust the voltage as needed (see 12.1 7100 General Specifications). If you are located outside
the United States, see Appendix F - Line Power Cord and Plug Wiring.
Turn the instrument on with the main power switch on the rear panel (see Figure 1.4). After
about 30 seconds, the Initializing Software screen will appear 2, followed by the Initializing
Motors screen.
The sipper will move up, all the way to the right, then over to the first installed module. If the
sipper does not move, make sure the packing material was removed (see 3.1 Unpacking, Remove
Packing Material). The Continue Initialization screen (see below) will appear.
2
The Initializing Software screen may remain for a few minutes when 3 sensor modules are installed.
3-4
Touch No
If you are not certain that the sipper alignment has been set, touch the [No] button. The Main
screen should appear similar to the one below. Note that the number of probes listed depends on
the number of currently installed sensor modules (two probes per module).
Menu Button
3.5.3 Calibrate Sipper

IT IS VERY IMPORTANT THAT THE SIPPER BE ACCURATELY ADJUSTED 3.
From the main screen, touch [Menu], then [Service], then [Sipper].
3
Misadjustment of sipper height can alter module volume and affect ISE measurement accuracy.
3-5
Select Sipper
The sipper will move to the Home position. The Sipper screen is used to align the Sipper with
each sensor module.
WARNING: Keep your hands clear of the sipper while the

instrument is in operation.
To make certain the Sipper is correctly aligned with each sensor module, follow the instructions
below.
Sensor Module 1
With the Calibrate Sipper menu displayed, touch the [Go To Module 1] button. The Sipper will
move to the sensor module 1 position. If your instrument does not have module 1 installed, skip
to the Sensor Module 2 section below.
3-6
Use the Arrow buttons to lower the Sipper and center it at the top of the cone shaped
opening in sensor module 1. If you cannot center the sipper by moving it left or right (it needs to
move in or out), see 8.6 Sipper Mechanical Alignment. With the tip of the Sipper exactly
centered and even with the top of the cone, press the Up Arrow button 2 times to raise the
sipper 2 steps.
Figure 3.1
Sipper Adjustment Position
When the sipper is properly aligned with sensor module 1, touch the [Set Well Alignment] button
to save the changes.
3-7
Sensor Module 2
Touch the [Go To Module 2] button. The Sipper will move to the sensor module 2 position. If your
instrument does not have module 2 installed, skip to the Sensor Module 3 section below.
sipper 2 steps (see Figure 3.1).
Sensor Module 3
Touch the [Go To Module 3] button. The Sipper will move to the sensor module 3 position.
3-8
Test Tube Holder

If your instrument has a turntable installed, skip to the Turntable section below.
Place the size test tube you intend to use in tube holder 1. If using multiple sizes, adjust the
sipper using the shortest test tube. Touch the [Go To Tubeholder] button.
3-9
The sipper will move to test tube holder 1. Use the Left and Right Arrow buttons to exactly
center the Sipper above the test tube.
Touch the Down Arrow repeatedly to lower the sipper into the test tube. Use the up and down
Arrow buttons to position the sipper just above the bottom of the test tube.
When the sipper is adjusted properly, touch [Set Tube Depth] to save the changes, then touch
[Close] to exit and return to the Main screen.
Turntable
If your instrument has a turntable installed, place a micro-centrifuge tube in turntable position 1.
Touch the [Go To Turntable] button.
The sipper will move to turntable position 1. Use the Left and Right Arrow buttons to
exactly center the Sipper above the tube.
Touch the Down Arrow repeatedly to lower the sipper into the tube. Use the up and down
Arrow buttons to position the sipper just above the bottom of the tube.
Your Sipper alignment is now complete.
3-10
3.5.4 Setup Instrument Parameters
Before operating the YSI 7100 MBS, you must set the instrument parameters. Once set, these
parameters are maintained in memory.
Menu Button
From the main display, touch the [Menu] button to display the menu selections.
Sequence
Touch [Sequence] to display the Setup Sequence screen as shown below in Figure 3.2.
The Setup Sequence screen is used to create sequences, select the location of the sample(s) and
the number of Replicates at each sample position, and enter sample IDs. Before you can select
sample locations, you must first create a new sequence as described below.
3-11
Figure 3.2
New Sequence
Touch the [New] button to create a new sequence. The alphanumeric keypad will open.
Enter a name for the sequence (one character at a time) by touching the corresponding buttons on
the keypad. If you make a mistake, use the [BS] button (backspace) to correct it one character at
a time, or the [Clear] button to delete all characters and start again.
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Touch the [OK] button to return to the Setup Sequence screen.
Select which tube holders the instrument will sample from by touching the tube holder’s name
(or by touching the Sample ID field if you would like to use sample IDs). The Sample ID box for
that tube holder will highlight to indicate it is selected.
Select a Tube Holder

by touching its name.
If you touched the Sample ID field, use the alphanumeric keypad to enter your sample ID, then
touch the [OK] button to return to the Setup Sequence screen.
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If the 7100 MBS is equipped with the optional YSI 7110 Turntable accessory, select a turntable
position by touching its name (or by touching the Sample ID field if you would like to use
sample IDs). The Sample ID box for that turntable position will highlight to indicate it is
selected.
Select a Turntable
position by touching
its name.
To select turntable positions higher than position 7, use the button to scroll the display down.
To quickly see all turntable positions that are selected, touch the [View] button.
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Selected positions are bold. Touch the [Close] button to return to the Setup Sequence screen.
Replicates
To change the number of sample Replicates, touch the Replicates number to open the numeric
keypad.
The numeric keypad consists of a title bar that describes which field is being modified (in this
case, Replicates), numeric buttons, and backspace, clear, cancel and OK buttons.
Title Bar
Backspace Button
Numeric Buttons Clear Button
Cancel Button
OK Button
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Enter the value (one digit at a time) by touching the corresponding buttons on the numeric
keypad. If you make a mistake, use the [BS] button (backspace) to correct them one digit at a
time, or the [Clear] button to delete all digits and start again.
When you have finished entering the value, touch the [OK] button to save your change and return
to the Setup Sequence display, or touch the [Cancel] button to abort.
When you have finished setting up the current sequence, touch the [Apply] button to save the
sequence.
Create additional sequences from scratch using the [New] button or use the [Copy] button to
create a copy of an existing sequence. See 4.1.3 Sequence for details.
After you have finished setting up all your Sequences, touch the [OK] button to return to the main
display.
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From the main display, touch the [Menu] button to display the Setup menu selections.
Sensor
From the Setup menu, touch [Sensor] to display the Setup Sensor screen as shown below.
Figure 3.3
3-17
The left side of the Sensors screen shows installed sensors (two per module) along with the
chemistries that are assigned to them. If no chemistry is assigned to a sensor, only the module
number and sensor letter are displayed. The currently selected sensor is highlighted. Figure 3.3
shows the display on an instrument with three sensor modules installed while Figure 3.4 shows
the display with 2 sensor modules installed.
Figure 3.4
The top of the screen (toward the right side) has four tabs, General, Sample, Auto-Cal and
Calibrate (hidden), which are described in detail in 4.1.4 Sensor.
NOTE: YSI has assigned default values for each of these parameters, but provides the flexibility
for the user to change values to optimize a particular application. DURING INITIAL SETUP,
YSI RECOMMENDS THAT YOU USE THE DEFAULT SETTINGS FOR THE
CHEMISTRIES YOU CHOOSE.
Note that any changes made on the right side of the screen only affect the sensor that is
currently selected (highlighted) on the left side.
The first sensor (1A for instruments with 3 modules, 2A for instruments with 2 modules or 3A
for instruments with 1 module) is already selected (highlighted) when you enter the Sensors
screen.
Module 1—ISE
Select the chemistry for sensor 1A by touching the arrow in the box to the right of the chemistry
field.
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Select Chemistry
A box will drop down displaying the available choices. Choose Ammonium by touching it.
Select the units for the Ammonium (1A) sensor by touching the arrow to the right of the units
field.
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A box will drop down displaying the available choices. Choose your selection by touching it.
Available selections are milligrams/liter (mg/L), milligrams/deciliter (mg/dL), grams/liter (g/L),
millimoles/liter (mmol/L) and percent, weight in grams to volume in milliliters, %(w/v).
Select sensor 1B by touching it on the left side of the screen.
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Select the chemistry for sensor 1B by touching the arrow to the right of the chemistry field. A
box will drop down displaying the available choices.
Choose Potassium by touching it.
Select the units for the Potassium 1B sensor by touching the arrow to the right of the units field.
A box will drop down displaying the available choices.
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Choose your selection by touching it.
NOTE: The End Point choice refers to the residence time of the sample in the Sensor Module.
The Default value is displayed. Do not change this value unless you are customizing an
application.
Module 2—Enzyme
Select sensor 2A by touching it on the left side of the screen.
field.
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Select Chemistry
A box will drop down displaying the available choices. Choose Glucose by touching it.
Select the units for the Glucose (2A) sensor by touching the arrow to the right of the units field.
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Available selections are milligrams/liter (mg/L), milligrams/deciliter (mg/dL), grams/liter (g/L),
millimoles/liter (mmol/L) and percent, weight in grams to volume in milliliters, %(w/v).
3-24
Choose Lactate by touching it.
Select the units for the Lactate (2B) sensor by touching the arrow to the right of the units field. A
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Choose your selection by touching it.
Module 3—Enzyme
field.
Select Chemistry
A box will drop down displaying the available choices.
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Choose Glutamate by touching it.
Select the units for the Glutamate (3A) sensor by touching the arrow to the right of the units
field.
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Choose Glutamine by touching it.
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Select the units for the Glutamine (3B) sensor by touching the arrow to the right of the units
field. A box will drop down displaying the available choices.
Choose your selection by touching it. Sample Tab
Touch the [Sample] tab to display the sample volume.
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With the Glutamine sensor still selected, touch the left arrow (directly below the Volume field)
five times to change the volume to 20.
After you have finished choosing all your selections for the Setup Sensor screen, touch the [OK]
button to save your changes and return to the main display.
3.5.5 Install Printer Paper

Remove the paper cover from top of the instrument by pulling up the rear edge of the cover.
Insert the end of the paper under the roller at the rear of the printer as shown below.
Figure 3.5
The outermost side of the paper on the roll should be facing down (see Figure 3.5). The printer
will automatically feed the paper in. After the paper is through the printer, make sure it is
straight. If it isn’t straight, twist the paper release (located on the right side of the printer behind
the paper feed knob) clockwise until it clicks, straighten the paper, then twist the paper release
counterclockwise to its original position.
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Paper
release
twisted
Paper
release
Feed the paper through the slot in the paper cover. Replace the paper cover by inserting the tabs
on the front edge into the slots on the instrument, then pushing down on the rear of the cover.
3.5.6 Prepare and Install Buffer Solution(s)

Caution: To prevent possible damage due to an electrostatic discharge, do NOT touch the
metal tips of the connectors located at the ends of the bottle leads. Handle only the
insulated section of the connectors.
Prepare the system buffer and fill the buffer bottle(s). Buffer is included in the starter supplies. In
this setup, use YSI 2357 buffer for enzyme modules and 7170 buffer for ISE modules. YSI 2357
is recommended for use with all YSI Enzyme Membranes except YSI 2786 Ethanol Membranes
(use YSI 2787), YSI 2725 Methanol Membranes (use YSI 1579), and YSI 2702 Galactose
Oxidase Membranes (use YSI 2705). Buffer bottle labels are included with the 7100. Apply them
to the buffer bottles as required. All YSI buffers may be ordered separately (see 11 Accessories
and Replacement Parts).
Prepare Buffer
» Place about 250 mL of reagent water (distilled or deionized) into a 500 mL flask, or
other clean container. Add one package of YSI 2357 Buffer Concentrate and stir.
NOTE: YSI 1579 Carbonate Buffer Concentrate (used with 2725 membranes) must
be reconstituted as instructed on the bottle.
» Add more reagent water until the total volume of solution is between 475 and 500
mL.
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» Stir as necessary, until the buffer chemicals have completely dissolved.
Install Buffer Solution(s)
» Remove the bottle cover from the top of the 7100.
» Disconnect the electrical lead from the top of one of the buffer bottles (B1–B4) and
remove the lid from the bottle. NOTE: When using the optional bar code scanner, be
sure that the bottle label matches the buffer you are using.
» IMPORTANT: When adding fresh buffer to the Buffer Supply Bottle or when
installing a new bottle of Calibrator Solution, make every effort to avoid
contamination of the lid and level sensor assemblies.
» Install the bottle(s) of 7170 buffer in location B1 and/or B2.
» NOTE: On instruments equipped with an ISE module, B1 and B2 should only
contain 7170 buffer solution. Do not install other types of buffer in these locations.
» Pour the prepared 2357 buffer into buffer bottle B3 and/or B4.
» Replace the bottle lid and reconnect the correct lead (B1–B4).
» Repeat this process for any additional buffer bottles (B1–B4) you want to use. The
7100 automatically selects the correct buffer for each sensor and switches to a new
bottle when the current bottle becomes empty or expires. The 7100 uses the oldest
buffer first.
Figure 3.6
Recommended Reagent Bottle Locations
NOTE: The bottle level sensors will not operate properly until the bottles have been
primed. See 3.5.10 Setup Bottles.
3.5.7 Install Calibrator Solution(s)

Caution: To prevent possible damage due to an electrostatic discharge, do NOT touch the
metal tips of the connectors located at the ends of the bottle leads. Handle only the
insulated section of the connectors.
Install the required calibrator solutions for the chemistries you want to measure. One or more of
the following YSI calibration standards will be provided in your starter supplies; YSI 7147
(Glucose and L-Lactate), YSI 7155 (L-Glutamate), YSI 71364 (L-Glutamine), YSI 7171
4
YSI 7136 Glutamine Calibration Standard must be reconstituted as instructed on the bottle.
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(Potassium), YSI 7172 (Ammonium), YSI 7190 (Sucrose), YSI 2790 5 (Ethanol), YSI 27265
(Methanol), YSI 7167 Xylose. YSI calibrators may be purchased separately.
» Unplug the electrical lead from the top of one of the empty calibrator bottles (C1–
C6) and remove the lid. For best results, install each calibrator in the location that
matches the sensor chemistry (calibrator well closest to the sensor). See Figure 3.7
for the location of calibrator wells with respect to each sensor. For a 3 module 7100
with an ISE module, install the calibrators as shown below.
» C1 7172 Ammonium
» C2 7171 Potassium
» C3 7147 Glucose/Lactate
» C5 7155 Glutamate
» C6 7136 Glutamine
» Mark the date of installation on the label of the new bottle of YSI calibrator solution.
» Screw the lid and level sensor assembly onto the new bottle of calibrator and place it
in the instrument compartment.
» Reconnect the correct electrical lead (C1–C6).
» Repeat this process for any additional calibrator bottles (C1–C6) you want to use.
The 7100 automatically selects the correct calibrator for each sensor and switches to
a new bottle when the current bottle becomes empty or expires. The 7100 uses the
oldest calibrator from the same module as the sensor first, but will use calibrator
from other modules if necessary.
» Replace the 7100 bottle cover.
Reference
C1 C6
1A 3B
Ammonium C2 C5
C3 C4
1B 3A
Potassium
2A 2B
Figure 3.7
Calibrator Well, Enzyme Sensor and ISE Locations
NOTE: The bottle level sensors will not operate properly until the bottles have been
primed. See 3.5.10 Setup Bottles.
5
YSI 2790 Ethanol Calibration Standard or 2726 Methanol Calibration Standard may be installed in a Cal Bottle location, but
evaporation through plastic components may be a problem. See 5.7 Ethanol or 5.10 Methanol for details.
3-33
3.5.8 Install Membranes on Enzyme Probes
Each active enzyme probe installed in your instrument is fitted with a protective "shipping
membrane" which must be removed and replaced with a new membrane.
Sensor modules are numbered 1 through 3 starting on the left. Therefore, the left probe in the
first (left) sensor module is probe 1A. See Figure 3.7.
One or more packs of YSI Immobilized Enzyme Membranes is provided in the starter supplies.
Each pack contains four membranes. Enzyme Membranes are color-coded for each type of
chemistry. It is important that you note which sensor module (1 through 3) and which probe, A
or B, you use to install specific Membranes. It will be necessary to assign chemistry to probe
during instrument parameter programming.
To install a membrane, first unscrew the appropriate enzyme probe retainer and gently pull the
probe out of the module. Remove the existing O-ring membrane assembly from the end of the
enzyme probe. A toothpick or soft pointed tool may be needed to unseat the old membrane. Be
careful not to scratch the enzyme probe face.
Examine the enzyme probe surface and remove any pieces of membrane that remained. Open a
cavity of the plastic membrane holder and rinse the membrane inside with a few drops of salt
solution (YSI 2392 or equivalent). Place one drop of salt solution on the enzyme probe face.
Using the plastic membrane holder, press the O-ring membrane assembly gently onto the probe
face (see Figure 3.8). Wipe excess salt solution from the probe body, then return the enzyme
probe to the sensor module. Finger tighten the probe retainer so that the O-ring seals the probe in
place. Do not overtighten. Repeat this procedure for the remaining enzyme probes.
Return the membrane holder to the foil pouch and refrigerate it. Note the expiration date on the
membrane package. You may want to maintain an instrument log book in which dates and lot
numbers of reagents are recorded, along with information from daily operational checks and
other relevant information (see 8.1 Daily Operational Checks).
Figure 3.8
Enzyme Membrane Installation
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3.5.9 Install Ion Selective Electrodes
The 7100 MBS is shipped without the Ion Selective Electrodes installed in the modules. To
install an ISE, first remove the packaging material and the cap from the ISE (save the cap for
later use).
Slide a black probe retainer over each ISE cable (threaded end first). Slide a white probe sleeve
(notched end out) over the end of each ISE.
Retainer
Place an o-ring (blue for ammonium; white for potassium; black for reference) into the end of
each sleeve, pushing the o-ring gently so that it is secured by the sleeve.
O-ring
Unscrew the three thumbnuts on top of the ISE module and carefully raise the module. Install the
reference electrode by screwing the probe retainer into the module (finger tighten only; do not
overtighten).
Reference Electrode
Thumb Nuts
Ammonium ISE
Potassium ISE
Install a stir bar in the module. Secure the ammonium and potassium ISEs in place (refer to
Figure 3.7 for location) by screwing the probe retainers into the module (finger tighten only; do
not overtighten). Install the module back on the waste chamber and secure it with the three
thumbnuts.
Connect the ISE cables to the matching color-coded sockets located at the bottom left corner of
the fluid bulkhead.
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Potassium—White
Ammonium—Blue
Reference—Black
3.5.10 Setup Bottles

Since it may take from several minutes to more than an hour to initially stabilize the probes when
setting up for the first time, now is a good time to prime the fluid system. You have already
reconstituted the appropriate buffer(s) and transferred it to the Buffer Bottle(s). You have also
installed the appropriate calibrator solution(s), and installed the appropriate enzyme
membrane(s) and ISEs in the sensor module(s). Next you will program the reagent information
into the 7100 MBS’ memory.
Select Bottle
From the main screen, touch [Menu], then [Service], then [Bottle]. The Service Bottle screen is
displayed.
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Select Detail Tab
Touch the Detail Tab to show the bottle details.
Select first Bottle location
Calibrator Bottles
Select (highlight) the first bottle (C1 for instruments with 3 modules, C3 for instruments with 2
modules, or C5 for instruments with one module) by touching it.
Reagent Number field
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Bar Code Entry
If you have the optional YSI 7181 Bar Code Scanner, scan the bar code on the selected bottle,
then skip the Manual Entry section below.
Manual Entry
If you do not have a bar code scanner, touch the Reagent Number field to open the numeric
keypad.
Enter the reagent number of the calibrator solution (one character at a time) by touching the
corresponding buttons on the keypad. If you make a mistake, use the [BS] button (backspace) to
correct it one character at a time, or the [Clear] button to delete all characters and start again.
Press the [OK] button on the numeric keypad to return to the Service Bottle screen.
3-38
Lot Number field
Touch the Lot Number field to open the alphanumeric keypad. A lot number must be entered in
order for the 7100 to track the expiration date and working life.
Enter the lot number of the calibrator solution (one character at a time) by touching the
corresponding buttons on the keypad. Touch [Shift] to enter numbers. If you make a mistake, use
the [BS] button (backspace) to correct it one character at a time, or the [Clear] button to delete all
characters and start again.
Press the [OK] button to return to the Service Bottle screen.
3-39
Select (highlight) the next bottle (C2 for instruments with 3 modules, C4 for instruments with 2
modules, or C6 for instruments with one module) by touching it.
Scan the bar code on the bottle or manually enter the Reagent and Lot numbers for this bottle as
described above. If you have not installed a bottle at this location, skip to the next bottle.
Select the next bottle (C3 for instruments with 3 modules, or C5 for instruments with 2 modules)
by touching it.
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described above.
Select the next bottle (C4 for instruments with 3 modules, or C6 for instruments with 2 modules)
by touching it.
described above. If you have not installed a bottle at this location, skip to the next bottle.
Select the next bottle (C5 for instruments with 3 modules) by touching it.
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described above.
Select the last bottle (C6 for instruments with 3 modules) by touching it.
described above.
3-42
Buffer Bottles
Select the location of buffer bottle B1 by touching it.
Select Bottle B1
Bar Code Entry

If you have the optional YSI 7181 Bar Code Scanner, scan the bar code on the bottle, then skip
the Manual Entry section below.
3-43
Manual Entry
keypad.
Enter the reagent number of the buffer solution (one character at a time) by touching the
Note that it is not necessary to enter a Lot Number for buffer.

Select the next installed buffer bottle by touching it.
3-44
Scan the bar code on the bottle or manually enter the Reagent number for this bottle as described
above.
Press the [OK] button to save the information for the bottles and return to the Main display.
3.5.11 Prime the Fluid System

displayed.
Select Bottle
Touch Initialize
3-45
Prime Calibrator Bottle(s)
On the left side of the display, select the first calibrator solution that you installed (C1 for
instruments with 3 modules, C3 for instruments with 2 modules, or C5 for instruments with one
module). General information about the selected calibrator is displayed on the right side of the
screen.
Touch the [Initialize] button. When prompted to “Set start of working life to today?”, touch [OK].
The instrument will prime the selected calibrator solution and set the start date for the working
life of the calibrator bottle to today.
Select the next calibrator solution that you installed (C2 for instruments with 3 modules, C4 for
instruments with 2 modules, or C6 for instruments with one module).
3-46
The instrument will prime the selected calibrator solution and set the start date for the working
life of the calibrator bottle to today.
Select the next calibrator solution that you installed (C3 for instruments with 3 modules, or C5
for instruments with 2 modules).
3-47
Touch the [Initialize] button, then touch [OK]. The instrument will prime the selected calibrator
solution and set the start date for the working life of the calibrator bottle to today.
Select the next calibrator solution that you installed.
3-48
Select the next calibrator solution that you installed.
3-49
Prime Buffer Bottle(s)
On the left side of the display, touch [B1] under Buffer (this is where you installed the first buffer
bottle). General information about the selected buffer will be displayed on the right side of the
screen.
Touch Initialize
Select Bottle B1
Touch the [Initialize] button, then touch [OK] to prime the Buffer and set the start date for the
working life of the bottle to today. The Sipper will move to the sensor module and prime the
fluid system with buffer solution.
3-50
Select the next buffer solution that you installed.
Touch the [Initialize] button, then touch [OK]. The instrument will prime the selected buffer
solution and set the start date for the working life to today.
Touch OK
After the fluid system is primed, touch the [OK] button to return to the Main display screen.
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3.5.12 Check Probe Currents
Touch the [Menu] button, then [Diagnostics] to display the diagnostics menu as shown below.
Touch [Probe] to enter the Probe Diagnostics screen.
Ion Selective Electrodes

Touch the [Flush] key to flush the ISE sensor module with buffer. Note that the 7100 will purge
buffer to waste if the incorrect buffer is in the line.
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Observe the voltage values. They must be between 0 and 60mV and stable. When the 7100 MBS
is first powered up, it may take an hour for the voltages to stabilize. Check the sensor module; it
should be full of buffer. If necessary, touch the [Flush] key to flush the module again.
Enzyme Probes
On the left side of the display, select the first enzyme module.
Touch the [Flush] key to flush the selected module with buffer. Observe the enzyme probe
current values (baseline). They must be below 5 nA and stable. When the 7100 MBS is first
powered up, it may take several hours for the baseline currents to drop below 5 nA. Check to see
if they are decreasing in value. Check the sensor module; it should be full of buffer. If necessary,
touch the [Flush] key to flush the sensor module again.
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On the left side of the display, select the next enzyme module.
Observe the enzyme probe current values as described above.

When the enzyme probe baseline currents are below 5 nA, touch the [Close] button to exit to the
main display.
3.5.13 Calibrate
The YSI 7100 MBS must be calibrated before running samples. From the main display, touch the
[Menu] button to display the main menu selections.
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Service
Touch [Service] to display the service selections as shown below.
Calibrate
Touch [Calibrate] to display the Calibrate Sensors screen.
Touch Ammonium
Select the Ammonium sensor by touching its name on the left side of the display. Notice that the
information for the sensor is now highlighted, along with the Potassium sensor (which is
required for ammonium measurement).
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Touch Glucose
Select the Glucose sensor by touching its name on the left side of the display.
Touch Lactate
Select the Lactate sensor by touching its name on the left side of the display.
Touch Glutamine
Select the Glutamine sensor by touching its name on the left side of the display (note that the
glutamate sensor also highlights). The information for all sensors should now be highlighted.
Touch the [OK] button to initiate calibration. The Calibration Status screen will appear.
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The 7100 MBS will attempt to calibrate the selected sensors. Calibration status is displayed on
the screen and printed. See 9.1 Printout Information for details.
The 7100 MBS attempts to calibrate each enzyme sensor up to 5 times before aborting
calibration for that sensor. An error message is displayed for any enzyme sensor that cannot be
calibrated. Enzyme sensors that fail calibration are not used for sample measurement.
The user must check the calibration report (example shown below) for ISE sensors and
determine if the ISEs are working properly. The Selectivity Coefficient range is zero to 0.25 for
Ammonium, and zero to 0.1 for potassium. The Slopes must be greater than 52. The 7100 will
flag values that are out of range with an asterisk (*), but will still allow sample measurement.
See 9.1 Printout Information, Ion Selective Electrodes for details. Depending on the application,
the 7100 may operate with an ammonium coefficient as high as 0.40, provided it passes the daily
checks in section 8.1 . However, it is the user’s responsibility to determine if operation is
acceptable for their specific application.
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==Calibration Report== Selectivity Coefficient—Ammonium
Module#1
NH4+
-----
K+
-----
Must be greater than zero, but less than 0.25
->Coef: 0.20 0.025
->Slp: 61.18 58.05
Selectivity Coefficient—Potassium
Dil. Ratio 28.0
10/25/2002 11:08:32 Must be greater than zero, but less than 0.1
======================
Brief Calibration Report

Slopes
Must be greater than 52
After calibrating all selected sensors, the 7100 returns to the main display.
3.5.14 Perform Daily Operational Checks

To ensure that your 7100 MBS is operating properly, perform the Membrane Integrity and
Linearity checks described in 8.1 Daily Operational Checks on a daily basis.
3.5.15 Sample
You may present a sample at any of the Test Tube Holders, the Manual Sample Station, or the
optional Turntable. Before sampling, verify that the samples are located in the positions that are
selected in the currently selected Sequence. See 4.1.3 Sequence for details.
Test Tube
Holders
Manual Sample
Station
Test Tube Holders

The test tube holders accept 12x75mm test tubes and standard 2mL conical centrifuge tubes.
Some 500μL centrifuge tubes can be used (see Figure 3.9). Any container other than this should
be sampled at the Manual Station. See 4.6.4 Manual Sample for details.
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12x75mm
test tube
500μL
centrifuge 500μL
centrifuge
tube
tube
Figure 3.9
For the purpose of demonstrating basic operation, choose the high level standards for the
chemistries you are set up to run. For example, if you have installed a YSI 2365 glucose
membrane, calibrate with YSI 7147 standard and use YSI 1531 standard (containing 9.00 g/L
glucose) to run as a sample.
Place each sample tube in a tube holder. Be very careful not to jar or push on the Sipper during
operation. You could disturb its critical alignment.
NOTE: The Sipper is not designed to pierce septa.
Before sampling, select a sequence and verify that the location of the samples matches the
selections made in the Setup Sequence screen. See 4.1.3 Sequence for details.
From the Main screen, touch the Start button.
Start Button
Select sequence
If any sensors are not calibrated, a warning message will appear. Follow the onscreen
instructions or see 9 Troubleshooting for details.
3-59
The 7100 MBS will run the selected sampling sequence. The Sipper moves to the sample test
tube and travels about 3 millimeters (1/8 inch) below the surface of the fluid, aspirates the
sample and returns to the sensor module.
The analyte values for the sample are displayed and printed. Compare the instrument value with
the labeled value of the YSI standard. The 'linearity' standard should be within ±5% of the label
value. For example, if you calibrated the 7100 MBS at 1.80 g/L glucose using YSI 7147 as the
calibrator solution, YSI 1531 (9.00 g/L) should read between 8.55 g/L and 9.45 g/L glucose. If
the reading falls outside these limits, recalibrate the sensor. After recalibrating, repeat the
analysis as directed above. If your reading is still outside this range, proceed for now. Later in
section 8.1 Daily Operational Checks, specific actions for nonlinearity will be addressed.
Sample ID
Sample Date/Time
Chemistry and
Units Sample Result
Scroll Buttons
Standby Mode
To conserve reagents, the 7100 MBS can be set up to automatically enter Standby Mode if no
sample is processed for 1 or more hours. You may manually enter Standby Mode immediately by
selecting Standby from the Service menu. While in Standby, the 7100 does not auto-calibrate and
the following screen is displayed.
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Touch anywhere on the screen to exit Standby Mode. See 4.1.1 System to set up Standby Mode.
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4 System Config ur a tion and Oper a tion
4.1 Setup Men u
Before operating the YSI 7100 MBS, you must set the instrument parameters. Once set, these
parameters are maintained in memory. These parameters are discussed below.
Menu Button
Touch the [Menu] button to display the setup menu selections as shown below.
System
4.1.1 System
Touch [System] to display the Setup System screen.
4-1
Arrow
The Setup System screen is used to select the type of sample and calibration reports, view the
instrument serial number, select the length of time the 7100 MBS will remain idle before
automatically going into Standby mode, and enable waste level detection.
To change the Sample Report, Calibration Report or Auto-Standby setting, touch the arrow to the
right of it.
Drop Down Box
The available selections are as follows:
Sample Report: Brief Calibration Report: Brief
Detailed Detailed
None None
Auto-Standby: Off
1 Hour
2 Hours
4 Hours
4-2
After you have finished making your selections, touch the [OK] button to save your changes and
return to the main display (or touch the [Cancel] button to abort and exit to the main display).
4.1.2 Clock
From the Setup menu, touch [Clock] to display the following screen.
The Setup Clock screen is used to set the time and date. To change any of the Time/Date fields,
touch the number to open the numeric keypad.
4-3
Enter the correct value (one digit at a time) by touching the corresponding buttons on the keypad.
If you make a mistake, use the [BS] button (backspace) to correct it one character at a time, or
the [Clear] button to delete all characters and start again.
When you have finished entering the value, touch the [OK] button to save the change and return
to the Setup Clock display (or touch the [Cancel] button to abort).
Repeat this procedure to make changes to any additional fields.

After you have finished setting the time and date, touch the [OK] button to save your changes and
return to the main display (or touch the [Cancel] button to abort and exit to the main display).
4.1.3 Sequence
From the Setup menu, touch [Sequence] to display the Setup Sequence screen as shown below.
The Setup Sequence screen is used to create sequences, select the location of the sample(s) and
the number of Replicates at each sample position, and enter sample IDs. Before you can select
sample locations, you must first create a new sequence as described below.
4-4
Figure 4.1
New Sequence
Touch the [New] button to create a new sequence. The alphanumeric keypad will open.
Enter a name for the sequence (one character at a time) by touching the corresponding buttons on
the keypad. If you make a mistake, use the [BS] button (backspace) to correct it one character at
a time, or the [Clear] button to delete all characters and start again.
4-5
Touch the [OK] button to return to the Setup Sequence screen.
Select which tube holders the instrument will sample from by touching the tube holder’s name
(or by touching the Sample ID field if you would like to use sample ID numbers). The Sample
ID box for that tube holder will highlight to indicate it is selected.
Select a Tube Holder

by touching its name.
If you touched the Sample ID field, use the alphanumeric keypad to enter your sample ID, then
touch the [OK] button to return to the Setup Sequence screen.
4-6
If the 7100 MBS is equipped with the optional YSI 7110 Turntable accessory, select a turntable
position by touching its name (or by touching the Sample ID field if you would like to use
sample ID numbers). The Sample ID box for that turntable position will highlight to indicate it is
selected.
Select a Turn Table

position by touching
its name.
To select turntable positions higher than position 7, use the button to scroll the display down.
To quickly see all turntable positions that are selected, touch the [View] button.
4-7
Selected positions are bold. Touch the [Close] button to return to the Setup Sequence screen.
Replicates
To change the number of sample Replicates, touch the Replicates number to open the numeric
keypad.
The numeric keypad consists of a title bar that describes which field is being modified (in this
case, Replicates), numeric buttons, and backspace, clear, cancel and OK buttons.
Title Bar
Backspace Button
Numeric Buttons Clear Button
Cancel Button
OK Button
4-8
Enter the value (one digit at a time) by touching the corresponding buttons on the numeric
keypad. If you make a mistake, use the [BS] button (backspace) to correct them one digit at a
time, or the [Clear] button to delete all digits and start again.
When you have finished entering the value, touch the [OK] button to save your change and return
to the Setup Sequence display, or touch the [Cancel] button to abort.
Replicates Order
When running replicate samples, select Order 111 to run all replicates from the first sample
location, then all replicates from the next sample location, etc. (111,222,333); or Order 123 to run
one sample from each location, then a second sample from each location, etc. (123,123,123).
4-9
When you have finished setting up the current sequence, touch the [Apply] button to save the
sequence.
Create additional sequences from scratch using the [New] button or use the [Copy] button to
create a copy of an existing sequence as shown below.
Copy Sequence
Touch the button to the right of the Sequence name to open the sequence selection box.
Touch the to open the

sequence selection box.
Select the sequence from

the list
Select the sequence you want to copy by touching its name.
4-10
Touch the [Copy] button. Enter a name for the new sequence, then touch [OK].
Make any changes to the new sequence.
Touch the [Apply] button to save the sequence.
4-11
Delete Sequence
Use the [Delete] button to delete the currently selected sequence as shown below.
Touch the button to the right of the Sequence name to open the sequence selection box.
Touch the to open the

sequence selection box.
Select the sequence from

the list
Select the sequence you want to delete by touching its name.
Touch the [Delete] button.
4-12
Touch [Yes] to delete the sequence. Note that if you delete all your sequences, you must create a
new sequence before you can select any sample locations.
After you have finished setting up all your Sequences, touch the [OK] button to return to the main
display.
4.1.4 Sensor
From the Setup menu, touch [Sensor] to display the Setup Sensor screen as shown below.
4-13
Figure 4.2
The left side of the Sensors screen shows installed sensors (two per module) along with the
chemistries that are assigned to them. If no chemistry is assigned to a sensor, only the module
number and sensor letter are displayed. The currently selected sensor is highlighted. Figure 4.2
shows the display on an instrument with three sensor modules installed while Figure 4.3 shows
the display with 2 sensor modules installed.
Figure 4.3
The top of the screen (toward the right side) has four tabs, General, Sample, Auto-Cal and
Calibrate, which are described below. Note that the Calibrate tab is hidden until the screen is
scrolled to the right (see Calibrate on page 4-18).
Note that any changes made on the right side of the screen only affect the sensor that is
currently selected (highlighted) on the left side.
NOTE: YSI has assigned default values for each of these parameters, but provides the flexibility
for the user to change values to optimize a particular application. DURING INITIAL SETUP,
YSI RECOMMENDS THAT YOU USE THE DEFAULT SETTINGS FOR THE
CHEMISTRIES YOU CHOOSE.
4-14
General
The General tab is already selected when you enter the Sensors screen. It is used to enable the
sensor and select the chemistry, end point and units for that sensor.
Select the sensor you want to setup by touching it on the left side of the screen. Note that first
sensor (1A for instruments with 3 modules, 2A for instruments with 2 modules or 3A for
instruments with 1 module) is already selected (highlighted) when you enter the Sensors screen.
Select the chemistry for the selected (highlighted) sensor by touching the arrow to the right of the
chemistry field.
Enzyme Sensor ISE
Currently available selections for enzyme sensors are Ethanol, Glucose, Glutamate, Glutamine,
Lactate, Sucrose, Galactose and Xylose. Available ISE selections are Ammonium and
Potassium.
4-15
Select the units for the sensor by touching the arrow to the right of the units field. A box will
drop down displaying the available choices. Choose your selection by touching it. Available
selections are mg/L, mg/dL, g/L, mmol/L and %(w/v).
The default End Point (end point time for the stabilized sensor reading) time is 30 seconds for
most chemistries (see 5 Chemistry Setup for details). The range is 10 to 80 seconds. Use the
default setting unless a particular application instruction specifies another value.
Sample
Touch the [Sample] tab to display the sample volume as shown below. The default Sample
Volume is 25μL for most chemistries (see 5 Chemistry Setup for details). The range is 10 to
50μL. Use the default setting unless a particular application instruction specifies another
value.
4-16
If your application requires a different sample volume, touch the arrows (directly below the
Volume field) to increment or decrement the value, or touch the value itself to open the
numerical keypad and enter the value directly.
NOTE: The sample volume for ISE sensors is fixed at 25μL.
If you have finished choosing all your selections for the Setup Sensor screen, touch the [OK]
button to save your changes and return to the main display (or touch the [Cancel] button to abort
all changes and exit to the main display).
Auto-Cal
Touch the [Auto-Cal] tab to display the Auto-Cal settings for the currently selected sensor. You
may alter any of these parameters to suit your application, however, you may compromise
precision and/or accuracy when doing so. YSI’s stated specifications are based on the default
settings shown below. These selections are provided as part of the overall concept of the 7100
MBS flexibility. You may have an application where 5% precision is acceptable if you can
guarantee that the instrument will not initiate a calibration at a critical sampling time.
4-17
Enzyme Sensor ISE
A checkmark to the left of each Auto-Cal parameter indicates that it is enabled. To change the
value of an Auto-Cal parameter, simply touch the value to open the numeric keypad.
Enter the new value (one digit at a time) by touching the corresponding buttons on the keypad. If
you make a mistake, use the [BS] button (backspace) to correct it one character at a time, or the
[Clear] button to delete all characters and start again.
After you have finished entering the new value, touch the [OK] button to save your change and
return to the Setup Auto-Cal display, or touch the [Cancel] button to abort the change.
NOTE: To disable an Auto-Cal parameter, touch the box to the left of the parameter and remove
the checkmark.
If you have finished choosing all your selections for the Setup Sensor screen, touch the [OK]
button to save your changes and return to the main display, or touch the [Cancel] button to abort
all changes and exit to the main display.
Calibrate
When you enter the Setup Sensors screen, the Calibrate tab is hidden from view. To access the
Calibrate tab, touch the button (at the top right corner of the display) to scroll the tab
selections to the right.
4-18
Tab Scroll Buttons
Calibrate Tab Visible
Touch the Calibrate tab to display the location of the calibrator solution for the currently selected
sensor. The volume of calibrator solution that is used during a calibration cycle is also displayed,
but cannot be changed (since it matches the Sample Volume).
4-19
If your application requires placing a calibration solution in a test tube (such as when measuring
ethanol or methanol), touch the arrow (directly to the right of the Location field) to open the
Location drop down box.
Select a tube holder Location for the calibrator solution.
Touch the Concentration field to open the numerical keypad, then enter the calibrator value.
4-20
After you have finished entering the new value, touch the [OK] button to save your change and
return to the Setup Calibrate display (or touch the [Cancel] button to abort the change).
After you have finished choosing all your selections for the Setup Sensor screen, touch the [OK]
button to save your changes and return to the main display (or touch the [Cancel] button to abort
all changes and exit to the main display).
4.1.5 Scheduler
From the Setup menu, touch [Scheduler] to display the Setup Scheduler screen as shown below.
Use the Setup Scheduler screen to schedule calibrations or standby mode to occur at a preset
time of day. Up to 5 actions can be scheduled. To schedule an action, touch the arrow to the right
of the Action field.
4-21
Select an Action by touching it. Available choices are Calibrate Bio-sensors, Calibrate ISE
sensors, Calibrate all sensors and Standby.
Set the time for the Action to occur by touching the Start Time field.
Enter the start time (24 hour format) using the numeric keypad.
4-22
If you want the action to only occur on weekdays, touch the Weekday Only checkbox.
Add any additional Actions as required. When you have finished entering actions, touch [OK] to
save your changes (or touch [Cancel] to abort) and return to the Main display screen.
4.1.6 Ports
From the Setup menu, touch [Ports] to display the Setup Ports screen as shown below.
4-23
The Setup Ports screen is used to set the communications parameters and select the type of data
for each serial port. Ports may either transmit sample results or can be set to accept remote
commands.
To change the communications settings, touch the arrow to the right of the Port field and select
the port. Note that any changes made will only affect the selected port.
The default settings for communications are:

Setting Default Available Selections
Data None None, Sample Brief, Remote Mode
Baud Rate 9600 4800, 9600, 19200, 38400, 57600, 115200
Data Bits 8 4, 5, 6, 7, 8
Parity None None, Odd, Even
Stop Bits 1 1, 1.5, 2
Handshaking None
Send Sample Data

To transmit sample data via the currently selected port, touch the arrow to the right of the Data
field. A box will drop down displaying available choices.
Select Sample Brief to transmit sample results via the selected serial port. Note that both ports
may be set to Sample Brief at the same time.
Remote Mode
To set up the 7100 for remote operation (remote control by an external host, such as the Groton
Biosystems ARS-100), touch the arrow to the right of the Data field and select Remote Mode
from the drop down list. Note that only one port can be set to Remote Mode at a time.
See 4.2.8 Remote for instructions on placing the 7100 in Remote Mode.
4-24
Touch [OK] to save your changes (or touch [Cancel] to abort) and return to the Main display
screen. See 4.4.2 Export, Example Data File for data format. See 6 Output for serial port
connections.
4.2 Ser vice Men u

Touch the [Menu] button, then [Service] to display the service selections as shown below.
4.2.1 Calibrate
From the Service menu, touch [Calibrate] to display the Calibrate Sensors screen.
4-25
The Calibrate Sensors screen displays the status of the sensors and allows the user to initiate
calibration. Select the sensor(s) you want to calibrate by touching the chemistry name on the left
side of the display. Notice that the information for selected sensors is highlighted.
After you have selected all the sensors you want to calibrate, touch the [OK] button to initiate
calibration (or touch [Cancel] to abort and return to the main display).
The Calibration Status screen will appear.
4-26
The 7100 MBS will attempt to calibrate all selected sensors. Calibration status is displayed on
the screen and printed.
The 7100 MBS attempts to calibrate each enzyme sensor up to 5 times before aborting
calibration for that sensor. An error message is displayed for any enzyme sensors that cannot be
calibrated. Enzyme sensors that fail calibration are not used for sample measurement.
The user must check the calibration report (example shown below) for ISE sensors and
determine if the ISEs are working properly. The Selectivity Coefficient range is zero to 0.25 for
Ammonium, and zero to 0.1 for potassium. The Slopes must be greater than 52. The 7100 will
flag values that are out of range with an asterisk (*), but will still allow sample measurement.
See 9.1 Printout Information, Ion Selective Electrodes for details. Depending on the application,
the 7100 may operate with an ammonium coefficient as high as 0.40, provided it passes the daily
checks in section 8.1 . However, it is the user’s responsibility to determine if operation is
acceptable for their specific application.
==Calibration Report==
Module#1 Selectivity Coefficient—Ammonium
NH4+ K+
----- ----- Must be greater than zero, but less than 0.25
->Coef: 0.20 0.025
->Slp: 61.18 58.05
Dil. Ratio 28.0
10/25/2002 11:08:32
======================
Must be greater than zero, but less than 0.1
Brief Calibration Report Slopes

Must be greater than 52
Module#1 Asterisk—indicates a value that is out of range
NH4+ K+
----- -----
->Coef: 0.28* 0.022
->Slp: 60.34 56.93
Dil. Ratio 28.0

10/25/2002 11:08:30
======================
4-27
1000 mg/L K, Module#1
NH4+ K+
----- -----
IB(mV): 28.01 26.97
PL(mV): 6.31 37.43
PS -0.46 0.71
ISES: 14.36 57.01
IB(mV): 28.09 27.03

PL(mV): 6.36 37.65
PS -0.87 0.46
ISES: 14.47 57.34
IB(mV): 28.10 27.06

PL(mV): 6.30 37.31
PS -0.54 0.06
ISES: 14.34 56.82
Avg: 6.32 37.46

Std Dv: 0.03 0.17
End Point 30 sec

Cal shift -0.91%
Sample size 25uL
Temperature 21.50C
500 mg/L NH4, Module#1

NH4+ K+
----- -----
IB(mV): 28.40 26.98
PL(mV): 24.97 1.42
PS 0.98 -0.23
ISES: 56.84 2.16
IB(mV): 28.50 26.97

PL(mV): 25.31 1.45
Selectivity Coefficient—Ammonium
PS 1.16 -0.35
ISES: 55.61 2.22 Must be greater than zero, but less than 0.25
IB(mV): 28.63 27.02
PL(mV): 25.09 1.42
PS 0.64 -0.45
ISES: 55.10 2.16
Avg: 25.12 1.43
Std Dv: 0.17 0.02 Must be greater than zero, but less than 0.1
---------------
->Coef: 0.20 0.025
->Slp: 58.18 61.05
Dil. Ratio 28.0

End Point 30 sec
Cal shift -2.38% Slopes
Sample size 25uL
Temperature 21.70C Must be greater than 52
10/25/2002 11:08:32
======================
Detailed Calibration Report
After calibrating all selected sensors, the 7100 returns to the main display.
4-28
4.2.2 Bottle
From the main screen, touch [Menu], then [Service], then [Bottle].
Select Bottle
The Service Bottles screen is displayed as shown below.
Select Bottle
Select a bottle by touching its name on the left side of the screen. General information about the
selected bottle is displayed on the right side of the screen.
Touch Detail tab
4-29
Touch the Detail tab to display details of the currently selected bottle.
Installing Standard YSI Bottles

If you have the optional YSI 7181 Bar Code Scanner, scan the bar code on the bottle, then skip
the Reagent Number and Lot Number entry steps below.
keypad.
4-30
Enter the Reagent number of the bottle (one character at a time) by touching the corresponding
buttons on the keypad. If you make a mistake, use the [BS] button (backspace) to correct it one
character at a time, or the [Clear] button to delete all characters and start again.
Lot Number field
Touch the Lot Number field to open the alphanumeric keypad.
Enter the lot number of the bottle (one character at a time) by touching the corresponding buttons
on the keypad. Touch [SHIFT] to enter numbers. If you make a mistake, use the [BS] button
(backspace) to correct it one character at a time, or the [Clear] button to delete all characters and
start again. Note that it is not necessary to enter a Lot Number for buffer.
4-31
Repeat this process for all the other bottles you have installed in your 7100. First select the
location of each bottle, then either scan in the bar code or enter the Reagent and Lot numbers.
All newly installed bottles must be initialized before the 7100 can use them. See Initialize Bottles
on page 4-36.
4-32
Installing Custom Calibrator Values
Custom tab
Touch the [Custom] tab to display the Custom Calibrant selections.
Select the custom calibrant chemistry by touching the arrow to the right of the chemistry field.
4-33
Concentration field
Touch the Concentration field to open the numeric keypad.
Enter the concentration of the custom calibrant (one character at a time) by touching the
4-34
Working Life field
Touch the Working Life field to open the numeric keypad.
Enter the working life of the custom calibrant (one character at a time) by touching the
4-35
Initialize Bottles
All newly installed bottles must be initialized before the 7100 can use them.
displayed.
On the left side of the display, select the bottle by touching it. General information about the
selected bottle is displayed on the right side of the screen.
Select Bottle
Touch Initialize
4-36
The instrument will prime the selected bottle and set the start date for the working life of the
bottle to today.
On the left side of the display, select the next new bottle and repeat the process until all newly
installed bottles have been initialized.
Touch the [OK] button to return to the Main display.
Remove
Use the [Remove] button to clear the settings for a bottle position that is no longer being used.
4-37
Remove Button
From the Service Bottles menu, select the bottle you want to remove. Touch the [Remove] button
to clear all information for the selected bottle.
Touch the [OK] button to save your changes and return to the Main display.
4.2.3 Stir Bar

From the main screen, touch [Menu], then [Service], then [Stir Bar].
Select Stir Bar
4-38
The Stir Bar screen is used to adjust the speed of the stir bars. Each stir bar has two operating
speeds: normal speed, where the stir bar rotates smoothly in the module and accelerated speed,
where the stir bar jumps to help clear air bubbles from the module during the flush cycle.
Note: The module must be full of buffer when adjusting stir speed. To fill the module with
buffer, see 4.3.1 Probe.
Select a module by touching its name on the left side of the display.
Select Module
Observe the stir bar in the selected module. If the stir bar is rotating smoothly, use the Up Arrow
Button to increase the speed until the stir bar is jumping. If the stir bar won’t jump, even with
the speed to maximum (speed indicator all the way up), replace it.
4-39
Increase Speed
Speed indicator
Decrease Speed
Touch the [OK] button to save the speed for the selected module.
Repeat this process for all other modules installed in your 7100. After you have adjusted the stir
speed for all modules, touch the [Close] button to return to the Main display.
4.2.4 Sipper
IT IS VERY IMPORTANT THAT THE SIPPER BE ACCURATELY ADJUSTED 6.
From the main screen, touch [Menu], then [Service], then [Sipper].
Select Sipper
The sipper will move to the Home position. The Sipper screen is used to align the Sipper with
each sensor module.
WARNING: Keep your hands clear of the sipper while the

instrument is in operation.
6
Misadjustment of sipper height can alter module volume and affect ISE measurement accuracy.
4-40
To make certain the Sipper is correctly aligned with each sensor module, follow the instructions
below.
Sensor Module 1
With the Calibrate Sipper menu displayed, touch the [Go To Module 1] button. The Sipper will
move to the sensor module 1 position. If your instrument does not have module 1 installed, skip
to the Sensor Module 2 section below.
sipper 2 steps.
4-41
Figure 4.4
Sipper Adjustment Position
Sensor Module 2
Touch the [Go To Module 2] button. The Sipper will move to the sensor module 2 position. If your
instrument does not have module 2 installed, skip to the Sensor Module 3 section below.
4-42
Sensor Module 3
Touch the [Go To Module 3] button. The Sipper will move to the sensor module 3 position.
4-43
Test Tube Holder

If your instrument has a turntable installed, skip to the Turntable section below.
Place the size test tube you intend to use in tube holder 1. If using multiple sizes, adjust the
sipper using the shortest test tube. Touch the [Go To Tubeholder] button.
The sipper will move to test tube holder 1. Use the Left and Right Arrow buttons to exactly
center the Sipper above the test tube.
Touch the Down Arrow repeatedly to lower the sipper into the test tube. Use the up and down
Arrow buttons to position the sipper just above the bottom of the test tube.
Turntable
If your instrument has a turntable installed, place a micro-centrifuge tube in turntable position 1.
Touch the [Go To Turntable] button.
4-44
The sipper will move to turntable position 1. Use the Left and Right Arrow buttons to
exactly center the Sipper above the tube.
Touch the Down Arrow repeatedly to lower the sipper into the tube. Use the up and down
Arrow buttons to position the sipper just above the bottom of the tube.
4.2.5 Touch Panel

From the Service menu, select [Touch Panel] to display the Service Touch Panel screen as shown
below.
The Service Touch Panel screen is used to test or calibrate the touch panel. The touch panel is
calibrated at the factory and should not require user calibration.
Calibrate
Touch the [Calibrate] button to display the Touch Panel Calibrate screen.
4-45

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YSI 7100 MBS

• 3½” Floppy Disk Drive

1.3 User Fea tur es

Calibrator and Printer

3½″ Disk Drive

1.5 Con tr ols

1.5.1 Display/Touch Panel

Probe Chemistries and Sample Results

Menu Button Instrument Status

Keyboard Port (Bar Code Scanner)

Start Pause Stop Menu Manual Sample Add Sample

Setup Service Diagnostics Data Help

Sequence Standby Probe Reports About

Remove Packing Material

1. Open slot, then rotate

Slide the packing material out the side of the instrument.

3.5 Ba sic Setup

3.5.2 Connect AC Power

3.5.3 Calibrate Sipper

WARNING: Keep your hands clear of the sipper while the

Test Tube Holder

Your Sipper alignment is now complete.

Select a Tube Holder

Numeric Buttons Clear Button

Select sensor 1B by touching it on the left side of the screen.

Choose Potassium by touching it.

Select sensor 2B by touching it on the left side of the screen.

Choose Lactate by touching it.

A box will drop down displaying the available choices.

Choose Glutamine by touching it.

Choose your selection by touching it. Sample Tab

Touch the [Sample] tab to display the sample volume.

3.5.5 Install Printer Paper

3.5.6 Prepare and Install Buffer Solution(s)

3.5.7 Install Calibrator Solution(s)

3.5.10 Setup Bottles

Touch the Detail Tab to show the bottle details.

Select first Bottle location

Reagent Number field

Press the [OK] button to return to the Service Bottle screen.

Bar Code Entry

Reagent Number field

Note that it is not necessary to enter a Lot Number for buffer.

3.5.11 Prime the Fluid System

Select the next calibrator solution that you installed.

Select the next calibrator solution that you installed.

Touch [Probe] to enter the Probe Diagnostics screen.

Ion Selective Electrodes

Observe the enzyme probe current values as described above.

Touch [Service] to display the service selections as shown below.

Touch [Calibrate] to display the Calibrate Sensors screen.

Brief Calibration Report

3.5.14 Perform Daily Operational Checks

Test Tube Holders

Drop Down Box

Repeat this procedure to make changes to any additional fields.

Select a Tube Holder

Select a Turn Table

Numeric Buttons Clear Button

Touch the to open the

Select the sequence from

Select the sequence you want to copy by touching its name.