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User manual Shimadzu LCsolutions for LC2010C with Chiral columns
Edition: 1.2 Date: 7-Oct-11 Page 1 of 5
Previous Edition: 1.1 Date: 26-Aug-11
Writen by: H.I.V. Amatdjais-Groenen
Approved by: Dr. Dennis W.P.M. Lowik
1 Responsibilities.
First person responsible: Mariëlle Delville.
Second person responsible: Helene I.V. Amatdjais-Groenen.
2 Definitions.
Read the information on the Instrumentation page of the website of the Bio-organic Chemistry department:
http://www.ru.nl/bio-orgchem/instrumentation/chromatography/hplc/
3 Starting the chiral HPLC and logging into LC Real Time Analysis.
1. Check solvent amounts of isopropanol and heptane.Make sure the solvent level is above the MINIMUM
level. Otherwise, fill to MAXIMUM level using new bottle HPLC grade heptane or IPA p.a. grade solvent.
For heptane ask Mariëlle Delville.
2. Switch computer & monitor on; start the program LabSolutions LCsolution (screen on picture 1 appears).
3. Click on the device 1(Instrument 1, picture 1).
4. Leave all settings and press the OK button (picture 2).
5. A screen like picture 3 will appear and you will hear a ‘beep’ as a confirmation of the communication
between the computer and the HPLC.
6. Copy and paste a general method you want to use from the methods folder to your own folder. Now it is
possible to change this method.
7. Push the ADVANCED button (green
arrow). Enlarge the method-window, by
clicking on the MAXIMIZE icon (red
arrow on picture 3).
Data acquisition
Fill in runtime (picture 4).
Click on [Apply to all acquisition times] button to
make sure the detector stops at the same time.
LC Time program
Used when a gradient or shut-down program is needed. Run time is
40 minutes
Pump
Picture 4: Total Run time.
In Mode the Low pressure gradient is selected.
Fill in solvent composition (picture 5).
Detector A
Fill in wavelength(s).
Check if lamp is ON.
Controller
Auto sampler
Check values (picture 6).
Auto purge
Check if degasser is selected
In this example:
B = 10%
Picture 7:
Actual parameters.
5 Performing Single Analysis.
Sample Name
Sample ID
4. Make sure all the data in step 4 are correct and push the OK button. This will start the measurement.
*C:\Labsolutions\Data\Yourfolder\Methods\Methodname.lcm
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Organic Chemistry SOP: HPLC-chiral-1.2
User manual Shimadzu LCsolutions for LC2010C with Chiral columns
Edition: 1.2 Date: 7-Oct-11 Page 4 of 5
Previous Edition: 1.1 Date: 26-Aug-11
Writen by: H.I.V. Amatdjais-Groenen
Approved by: Dr. Dennis W.P.M. Lowik
7 Shutdown procedure.
5. Fill in your Batch file and your last sample must be the shut-down sample (picture 13).
6. Vial# is –1 (this means NO injection).
7. The method file is the shut-down method you checked (!!!!).
8. The data file is a NEW, NOT EXISTING file.
9. Save the batch file, and start it.