Stratum Spinosum
The stratum spinosum lies above the basal layer and is
composed of keratinocytes, which differentiate from the
basal cells beneath them. The keratinocytes produce
keratin, a fibrous protein that is the major component
Melanocytes
Melanocytes are dendritic, pigment-producing cells located
in the basal cell layer (Figs. 2.4 and 2.5). They protect the
FIGURE 2.3 Ichthyosis vulgaris – a common autoimmune, inherited dry
skin condition secondary to deficient filaggrin production. Note “fish-like” skin from ultraviolet radiation. Individuals with little or no
scale on the anterior shin. pigment develop marked sun damage and numerous skin
cancers. The dendrites extend into the stratum spinosum
and serve as conduits, through which pigment granules are
transferred to their neighboring keratinocytes. The granules
Granular cells contain keratohyalin and lamellar granules.
are termed melanosomes, and the pigment within is melanin,
which is synthesized from tyrosine. Melanosomes are pref-
erentially situated above the nucleus to protect the DNA.
Stratum Corneum
A remarkably abrupt transition occurs between the viable, People of all races have a similar number of melanocytes.
nucleated cells at the top of the granular cell layer and the The difference in skin pigmentation depends on (1 ) the
dead cells of the stratum corneum (Fig. 2.4 ). The cells in number and size of the melanosomes and (2) their dis-
the stratum corneum are large, flat, polyhedral, plate-like persion in the skin. In darkly pigmented skin, melano-
envelopes filled with keratin. They are stacked in vertical somes are larger in size and more numerous compared
layers that range in thickness from 1 5 to 25 layers on most with melanosomes in lightly pigmented skin. Sunlight
body surfaces to as many as 1 00 layers on the palms and stimulates melanocytes to increase pigment production
soles. The cells are held together by a lipid-rich cement in a and disperse their melanosomes more widely.
fashion similar to “bricks and mortar.” The tightly packed,
keratinized envelopes in the stratum corneum provide a Langerhans Cells
semi-impenetrable layer that constitutes the major physi- Langerhans cells are dendritic cells in the epidermis
cal barrier of the skin. that have an immunologic function (Fig. 2.4 ). They are
Stratum
corneum
Stratum
granulosum
Langerhans cell
Stratum
Melanocyte
spinosum
Basal cell
layer Dermal
papilla
Merkel cell
Rete ridge
FIGURE 2.4 Epidermis.
DERMIS 5
Merkel Cells The dermis is a tough, but elastic, support structure that
Merkel cells are located in the basal cell layer. They are contains blood vessels, nerves, and cutaneous appen-
more numerous on the palms and soles and are connected dages. It provides structural integrity and is biologically
to keratinocytes by desmosomes. Merkel cells function as active by interacting and regulating the functions of cells
mechanoreceptors. Merkel cell carcinoma is a rare skin (i.e., tissue regeneration). The dermis ranges in thick-
cancer with a high mortality rate, as discussed in Chapter 5. ness from 1 to 4 mm, making it much thicker than the
epidermis, which in most areas is only about as thick
as this piece of paper (Fig. 2.7). The dermal matrix is
Dermal–Epidermal Junction – The Basement composed primarily of collagen fibers (principal compo-
Membrane Zone nent), elastic fibers, and ground substance (now called
The interface between the epidermis and dermis is called extrafibrillar matrix), which are synthesized by dermal
the basement membrane zone. With light microscopy, fibroblasts. Collagen accounts for 70% of the dry weight
it is visualized only as a fine line. However, electron of skin. Collagen and elastic fibers are fibrous proteins
6 SECTION 1 CHAPTER 2 STRUCTURE AND FUNCTION OF THE SKIN
that form the strong, yet compliant skeletal matrix. In the Nerves and blood vessels course through the dermis, and
uppermost part of the dermis (papillary dermis), collagen a layer of subcutaneous fat lies below it (Fig. 2.9).
fibers are fine and loosely arranged. In the remainder
of the dermis (reticular dermis), the fibers are thick
and densely packed (Fig. 2.8). Elastic fibers are located Free nerve endings are the most important sensory receptors.
primarily in the reticular dermis, where they are thinner
and more loosely arranged than collagen fibers. The
extrafibrillar matrix fills the space between fibers. It is a Nerves
non-fibrous material made up of several different muco- The skin is a major sensory receptor. Without the sensa-
polysaccharide molecules, collectively called proteoglycans tions of touch, temperature, and pain, life would be less
or glycosaminoglycans. The extrafibrillar matrix imparts to interesting and more hazardous. Sensations are detected
the dermis a more liquid quality, which facilitates move- in the skin by both free nerve endings and more com-
ment of fluids, molecules, and inflammatory cells. plicated receptors that are corpuscular in structure. The
free nerve endings are the more widespread and appear
to be more important. The nerve supply of the skin is
Structural components of the dermis:
1. Collagen
2. Elastic fibers
3. Extrafibrillar matrix
Papillary dermis
Reticular dermis
FIGURE 2.7 Systemic scleroderma – an increase in the number and activity FIGURE 2.8 Papillary dermis – fine, loose collagen strands. Reticular
of fibroblasts produces excessive collagen and results in dermal thickening. dermis – thick and dense collagen strands.
Epidermis
Nerve
Dermis
Blood
vessels
Subcutaneous
fat
SKIN APPENDAGES
Key Points
1. Eccrine glands help to regulate body temperature
2. Apocrine sweat glands depend on androgens for their
development
3. The stem cells of the hair follicle reconstitute the nonperma-
nent portion of the cycling hair follicle
FIGURE 2.10 Herpes zoster – reactivation of varicella-zoster virus in sen- 4. Sebaceous glands are under androgen control
sory nerve ganglia results in a painful, vesicular, dermatomal eruption. 5. Nails, like hair, are made of keratin
HAIR FOLLICLE
In most mammals, hair serves a protective function, but
in humans it is mainly decorative. FIGURE 2.12 Alopecia areata – autoimmune condition resulting in
nonscarring circular patches of alopecia.
Hair follicles are distributed over the entire body surface,
except the palms and soles. Hair comes in two sizes:
hair follicle stem cells, a new hair matrix is formed at the
(1 ) vellus hairs, which are short, fine, light colored, and
bottom of the follicle, and the cycle is repeated (Fig. 2.1 2).
barely noticeable; and (2) terminal hairs, which are thicker,
At any time, 80% to 90% of scalp hair is in the anagen phase
longer, and darker than the vellus type. Terminal hairs in
and 1 0% to 20% is in the telogen phase, thus accounting
some locations are hormonally influenced and do not
for a normal shedding rate of 25 to 1 00 hairs per day.
appear until puberty (e.g., beard hair in males, and pubic
and axillary hair in both sexes).
Normally, 25 to 100 hairs are shed from the scalp each day.
Types of hair:
1. Vellus (light and fine) As shown in Fig. 2.11, the hair follicle is situated in the
2. Terminal (dark and thick) dermis at an angle. Not shown is an attached arrector pili
muscle. When this muscle contracts, the hair is brought
into a vertical position, giving a “goose flesh” appearance
A hair follicle can be viewed as a specialized invagination
to the skin. The stem cells of the hair follicle are located
of the epidermis (see Fig. 2.11 ), with a population of cells
in the “bulge” area of the follicle, where the arrector pili
at the bottom (hair bulb) that are replicating even more
muscle inserts into the hair follicle, and in the dermal
actively than normal epidermal basal cells. These cells
papilla. The stem cells are important for reconstituting
constitute the hair matrix. As with basal cells in the epi-
the nonpermanent portion of the cycling hair follicle and
dermis, the matrix cells first divide and then differentiate,
play a role in reconstituting the epidermal cells.
ultimately forming a keratinous hair shaft. Melanocytes
in the matrix contribute pigment, the amount of which
determines the color of the hair. As the matrix cells con- Sebaceous Glands
tinue to divide, hair is pushed outward and exits through Sebaceous glands produce an oily substance termed
the epidermis at a rate of about 1 cm per month. Hair sebum, the function of which is unknown. In fact, the skin
growth in an individual follicle is cyclical, with a growth of children and the palmar and plantar skin of adults
(anagen) phase, a transitional (catagen) phase, and a rest- function well without sebum.
ing (telogen) phase. The lengths of the phases vary from Sebaceous glands are part of the pilosebaceous unit and so
one area of the body to another. On the scalp, for exam- are found wherever hair follicles are located. In addition,
ple, the anagen phase lasts for about 3 years, the catagen ectopic sebaceous glands are often found on mucous
phase for about 3 weeks, and the telogen phase for about membranes, where they may form small yellow papules
3 months. The length of the anagen phase varies from called Fordyce spots. In the skin, sebaceous glands are
individual to individual, explaining why some persons most prominent on the scalp and face, and are moder-
can grow hair longer than others. ately prominent on the upper trunk. The size and secre-
tory activity of these glands are under androgen control.
Hair growth cycles through growth (anagen), transitional (cata- The sebaceous glands in newborns are enlarged owing
gen), and resting (telogen) phases. to maternal hormones, but within months, the glands
shrink (Fig. 2.1 3). They enlarge again in preadolescence
from stimulation by adrenal androgens and reach full
At the end of the anagen phase, growth stops and the size at puberty, when gonadal androgens are produced.
hair follicle enters the catagen and telogen phase, during
which the matrix portion and lower two-thirds of the hair
follicle shrivels and the hair within the follicle is shed. Sebaceous glands are androgen dependent.
Subsequently, through mesenchymal interaction with the
SKIN APPENDAGES 9
FIGURE 2.13 Infantile acne – a common disorder affecting the pilose- FIGURE 2.15 Lichen planus – an inflammatory condition that normally
baceous unit. Maternal androgens are influential. affects the skin and mucous membranes, but can affect the nail matrix and
cause dystrophic nails.
3. The epithelium of the nail bed produces a minimal evidenced by metabolic disturbances in obese children
amount of keratin, which becomes tightly adherent and adolescents with peripheral insulin resistance. Recent
to the bottom of the nail plate. The pink color of evidence supports the role of adipose-derived stem cells
a nail is due to the vascularity in the dermis of the in wound healing, hair follicle support/growth, and pro-
nail bed. tection against photoaging. Within the subcutaneous
4 . The epidermis of the hyponychium underlies the free fat layer, aggregates of fat cells (lipocytes) are separated
distal edge of the nail plate. Stratum corneum pro- by fibrous septa that are traversed by blood vessels and
duced there forms a cuticle to seal the junction of nerves (Fig. 2.1 6).
the distal nail bed and nail plate.