BMS 116
Laboratory # 2: Muscle, Bone and Cartilage
Laboratory Exercises
o Learn to identify and distinguish between skeletal, cardiac and smooth muscle
o Learn to recognize the major cell types associated with bone and cartilage
(including the cells that line these tissues),
o Learn to identify the principle structural and functional features of endochondral
bone formation.
longitudinal section
cross-section
Muscle fiber
! 1"
b) These pink-stained circles are arranged into elongated bundles. What are these
bundles called?
Note: Muscle > fascicles > fibers > fibrils> actin and myosin
epimysium
perimysium
endomysium
Mostly between the perimysium containing muscle fascicles, but pierces all layers to
supply muscle with blood
! 2"
CARDIAC MUSCLE SLIDE (Wheater’s p.116--121) (See Slide Online)
1. Compared to skeletal muscle, how are nuclei in cardiac muscle cells distinguished?
Nuclei are mostly mono-nucleiated and rounder, also not in the periphery
2. Cardiac muscle is composed of muscle cells (cardiomyocytes) that are long and
cylindrical. What is the name of the structure that separates the ends of adjacent cardiac
muscle cells? (see pg 117 Wheater’s).
Intercalated discs
intercalated disc
nuclei
! 3"
JEJUNUM SLIDE (Wheater’s p.112--115, p.266) (See Slide Online)
1. Smooth muscle fibers can be examined using your jejunum slide. Find the
smooth muscle fibers and compare their appearance with cardiac and skeletal
muscle examined previously.
a) Find these different layers of smooth muscle in the jejunum wall. How do
they differ in appearance?
See above
! 4"
TRACHEA SLIDE (See Slide Online)
dense CT (perichondrium)
1. Using the naked eye, then the 4x microscope objective, locate the darkly stained
tracheal cartilage. Increase the magnification, focusing at the periphery of the
cartilage with the 10x lens, then 40x as needed. At the periphery of cartilage, note the
flattened, closely adherent layer of connective tissue; this specialized connective
tissue is called the perichondrium. This tissue serves as the source for what type of
cells? (Wheater’s p.187)
Chondroblasts are located in the perichondrium. These chrondroblasts are precursors that
can still move.
2. Beneath the perichondrium is developing and developed cartilage tissue. What is this
type of cartilage called (Wheater’s p. 186)?
chondroblast
Hyaline cartilage (dark purple)
Chondroblasts (on the periphery) mature into chondrocytes (in the center of hyaline
cartilage). Chondroblasts have a larger nucleus and less cytoplasm; chondrocytes have
a larger cytoplasm to nucleus ratio.
! 5"
4. Within the tracheal cartilage, the chondrocytes are surrounded by an abundant darkly
stained hyaline cartilage.
Type II collagen
b) Which cells are responsible for synthesizing and maintaining the hyaline
cartilage matrix?
Chondrocytes
! 6"
FETAL FINGER SLIDE (See Slide Online)
Proliferating chondrocytes
Cells get bigger by gaining more cytoplasm; also become more disperse
bone marrow
4. What are the three cell types found in bone (p.
189)?
Haversian canals
! 8"