Kate Bruce
Mrs. Stevens
Physical Science
12 December 2018
Have you ever wondered what the dirtiest part of your bathroom is? I wanted to figure it
out. I wanted to know which appliance should be cleaned most frequently. For my experiment, I
measured the amount of bacteria in three different places in the bathroom. I thought that if I took
bacteria from a toilet seat, toilet handle, and sink faucet, then a sink faucet would have the most
bacteria.
Most bacteria can adapt to all conditions and temperatures, but it grows best with certain
conditions. Bacteria need nutrients and get it from sunlight using metabolic methods. It also
needs a source of water. Bacteria grows best at a pH of 6.0 to 8.0. Some bacteria need little to no
oxygen, but others need a lot. It grows best with temperatures from 40 degrees F to 150 degrees
F (Taylor). The area should be protein rich, warm, and moist. Only some bacteria thrive in
extremely cold and hot environments (Lesson). In all, bacteria grows the same way we do by
There is a very specific way to raise bacteria on a petri dish. First, put 10 microliters of
bacteria into a test tube. Transfer 10 microliters of that into a 90 microliter test tube. Then, repeat
and mix. Put 10 microliters of the solution onto an agar plate. Repeat with two other plates, and
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put it in an incubator. The colonies should be visible in 16 hours (Lesson). Keep everything
sterile and spread the agar onto the petri dish (Gaunt). Doing this will bring the best results.
There are a few ways to count bacteria growth. To directly count bacteria, use a
divided into different sized cubes. This will show the most precise results (Boundless). Another
way to measure bacterial growth is by plating. Plating is when you take a bunch of bacteria and
dilute it over and over. Place it on a petri dish and then count the colonies. Each bacteria will
form one culture (Wintermute). The easiest way to measure bacteria is by the size of the culture.
Bacteria can be very beneficial. The bacteria an organism’s skin, airways, and digestive
system protect them from catching diseases. Bacteria in the digestive and respiratory system help
the immune system too. These types of bacteria help regulate the respiratory and digestive
systems. Bacteria also breaks down carbohydrates and protect cells from invading pathogens.
Bacteria can also be found in food, such as yogurt (Gaunt). When there is a healthy amount of
good bacteria in the body, it will actually help keep bad bacteria out (Yttri). Bacteria decomposes
dead animals and plants to obtain their nutrients. This is why dead organisms aren’t laying
around. Bacteria is also in lots of food, such as yogurt (Gaunt). In all, bacteria can be very useful.
There are many key elements that affect bacterial growth. On of the important factors is
nutrient concentration. If the culture is rich in nutrient concentration, the bacteria grows faster.
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Temperature is also another key factor. Bacteria needs to grow in an area that has healthy
temperature from 50 to 150 degrees fahrenheit. If the bacteria is in an area above that
temperature, cellular enzymes and proteins denature. If the bacteria is in an area below that
temperature, the cell membrane will solidify. The pH levels can also affect bacterial growth. The
pH should be from 6.0 to 8.0. The pH affects ionic properties, and most grow at neutral (Karki).
The area bacteria is growing in should be warm, dark, and moist (Gaunt).
Even though most bacteria can be beneficial, it can also be harmful. Bad bacteria is called
a pathogenic. Pathogens can invade the cells of an organism, which can lead to diseases. Poor
hygiene and unclean water lead to bacterial infections. Bacteria invade living tissues inside
people. Harmful bacteria can cause some forms of pneumonia and strep throat (Gaunt).
I thought that if I take bacteria from a toilet seat, toilet handle, and sink faucet, then a
sink faucet will have the most bacteria, and I found out that I was correct, and a sink faucet does
have the most bacteria. The average amount of bacteria on a toilet seat was 0.6 cm. The average
amount of bacteria on a toilet handle was 0.3 cm. The sink faucet had an average amount of 0.5
cm. It proved how dirty bathrooms already are. This experiment proved to me that I should clean
Works Cited
www.sciencelearn.org.nz/resources/588-bacteria-good-bad-and-ugly.
courses.lumenlearning.com/boundless-microbiology/chapter/counting-bacteria/.
Karki, Gaurab. “Factor Affecting Bacterial Growth -.” - A Complete Notes for Students, 28 July
2017, www.onlinebiologynotes.com/factor-affecting-bacterial-growth/.
www.extension.iastate.edu/foodsafety/L1.6.
Taylor, Stacy. “What Three Conditions Are Ideal for Bacteria to Grow?” Sciencing.com,
Wintermute, Jake. “Course 2: Your First GMO – Synthetic Biology 1.” Synthetic Biology 1,
syntheticbiology1.com/courses/your-first-gmo/.
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Yttri, Jennifer. “Bacteria: the Good, the Bad, and the Ugly.” National Center for Health