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What is Aseptic Technique?

Bacteria are everywhere, and some are good for us while others are harmful. Bacteria, viruses,
and other microorganisms that cause disease are called pathogens. To protect patients from
harmful bacteria and other pathogens during medical procedures, healthcare providers use
aseptic technique.

Aseptic technique means using practices and procedures to prevent contamination from
pathogens. It involves applying the strictest rules to minimize the risk of infection. Healthcare
workers use aseptic technique in surgery rooms, clinics, outpatient care centers, and other health
care settings.

In the Microbiology lab we use aseptic technique to:

• Prevent contamination of the specific microorganisms we are working with.
• Prevent contamination of the room and personnel with the microorganisms we are working

1. Atmosphere
2. The breath
3. The hands
4. Clothing
5. Hair
6. Working surface
7. Equipment

•Sterilization- refers to any process that eliminates, removes, kills, or deactivates all forms of
life and other biological agents present in a specified region, such as a surface, a volume of fluid,
medication, or in a compound such as biological culture media
•Disinfection-does not necessarily kill all microorganisms, especially resistant bacterial spores;
it is less effective than sterilization, which is an extreme physical and/or chemical process that
kills all types of life.
•Antisepsis- the practice of using antiseptics to eliminate the microorganisms that cause disease.

What needs to be sterilized in the process of antiseptic technique?

1. Culture Media
2. Fluids used in the labs
3. Reagents
4. Laboratory containers
5. Laboratory equipment