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BIO5TEC 2019 - Techniques in Biotechnology Semester 1- SMBB/SMBM/SMBT/SGBB/SMNT

Week, Lecture: 2 hrs Workshop: 3 hrs


Assessment
Dates (Wed 10am-12pm, Undercroft) (Thursday 10am-1pm, TLC-115)

INTRODUCTION/ORIENTATION WEEK
4 - 8th March
1 Workshop worksheet [wks 2, 3, 4, 5,
Introduction Suzi Cutts Foundations of biotechnology
13th& 14th March 8, 9, 10] total of 40% assessment
Protein-based biotechnology Con Panousis (CSL Limited) Suzi Cutts
2 Biotechniques for analysis of gene function PROTEIN/GENE FUNCTION, GENE
20 & 21st March
th
Mark Bleackley KNOCKOUT/DELETION
Fluorescence microscopy & antibody-based approaches
3 FLUORESCENCE MICROSCOPY & ANTIBODY-
for protein characterisation in cells
27th & 28th March BASED APPROACHES
Peter Lock
Techniques in protein biophysics #1:
4 Protein purity, concentration determination, and SDS-PAGE, UV-VIS SPECTROSCOPY & TANDEM
3rd & 4th April primary structure analysis MASS SPECTROMETRY
Jason Paxman
Techniques in protein biophysics #2: CD SPECTROSCOPY AND ANALYTICAL
5
Secondary & quaternary structure analyses ULTRACENTRIFUGATION
10th & 11th April
Jason Paxman
6 Web-based tools for characterising protein sequences Presentation assignment due – 20%
ORAL PRESENTATIONS
17th & 18th April Thomas Shafee ~10 groups working in pairs

7 MID-SEMESTER BREAK

8 X-ray crystallography for protein tertiary structure


X-RAY CRYSTALLOGRAPHY METHODS
1st & 2nd May characterisation Begoña Heras
9 Applications of biotechnology – engineering of proteins
PROTEIN ENGINEERING
8th & 9th May Thomas Shafee
10 Use of ITC and SPR to characterise protein-protein ISOTHERMAL TITRATION CALORIMETRY AND
15 & 16th May
th
interactions Marc Kvansakul SURFACE PLASMON RESONANCE
11 ORAL PRESENTATIONS ORAL PRESENTATIONS Presentation assignment due – 20%
22nd & 23rd May Revision Tutorial ~20 groups working in pairs
12 STUDY BREAK

13 EXAM (OPEN BOOK) Exam (40%) 1.5 hours –


6th June LIMS1-101 (Note alternative venue) 4 choices from 6 topics

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