MS9-5 Manual de Uso Inglés PDF

MS9-5 H AUTOMATIC
FULL DIGITAL
CELL COUNTER
MS9-5 H
OPERATOR MANUAL
15/04/2003
MELET SCHLOESING LABORATOIRES © 2003

MS9-5 Operator manual 3
TABLE OF CONTENTS
Part I Introduction 6
1 Setting
...................................................................................................................................
up advice 6
2 Switching
...................................................................................................................................
on 6
Plugging in ......................................................................................................................................................... 7
Initialisation .........................................................................................................................................................
of the system 7
Main display
......................................................................................................................................................... 11
Part II Analysis 18
1 Carrying
...................................................................................................................................
out an analysis 18
Testing the .........................................................................................................................................................
blood sample 18
Preparation......................................................................................................................................................... 19
Analysis identification
......................................................................................................................................................... 21
Starting an analysis
......................................................................................................................................................... 23
Analysis cycle
......................................................................................................................................................... 24
Next analysis
......................................................................................................................................................... 26
2 Consulting
...................................................................................................................................
an analysis 27
Recalling the
.........................................................................................................................................................
day’s analysis 27
Search engine
......................................................................................................................................................... 30
3 Exporting
................................................................................................................................... 35
Exporting the
.........................................................................................................................................................
day’s analysis 35
Search engine
......................................................................................................................................................... 37
Part III Identification 40

1 Patient
................................................................................................................................... 40
2 Work
...................................................................................................................................
list 42
3 Operator
................................................................................................................................... 45
4 Department
...................................................................................................................................
and division 46
Part IV Calibration 50
1 Presentation
................................................................................................................................... 50
2 Manual
...................................................................................................................................
calibration 50
3 Automatic
................................................................................................................................... 51
4 Target
...................................................................................................................................
Values 54
5 Probe
...................................................................................................................................
voltage 55
Part V Quality control 60

1 Presentation
................................................................................................................................... 60
2 Target
...................................................................................................................................
values 60
3 Analysis
................................................................................................................................... 64
4 Consultation
................................................................................................................................... 65
Part VI Parameters 68
1 Presentation
................................................................................................................................... 68
2 Display
...................................................................................................................................
formats 68
3 Printing
...................................................................................................................................
formats 72
4 Fluidics
................................................................................................................................... 76

4 MS9-5 Operator manual
5 System
................................................................................................................................... 79
Tab system......................................................................................................................................................... 81
Hb tab ......................................................................................................................................................... 82
Sensitivity ......................................................................................................................................................... 83
Serial configuration
......................................................................................................................................................... 85
Parameters ......................................................................................................................................................... 86
View ......................................................................................................................................................... 86
Identification
......................................................................................................................................................... 86
Blank ......................................................................................................................................................... 89
Password ......................................................................................................................................................... 91
Export ......................................................................................................................................................... 92
6 Units
................................................................................................................................... 94
Regional Parameters
......................................................................................................................................................... 94
Display format
......................................................................................................................................................... 96
Part VII Services 100

1 Banks
................................................................................................................................... 100
Presentation
......................................................................................................................................................... 100
Selection ......................................................................................................................................................... 100
Modification
......................................................................................................................................................... 101
Threshold .................................................................................................................................................. 103
Gain Modification
.................................................................................................................................................. 104
Modifying the..................................................................................................................................................
counter’s time 105
Modifying the..................................................................................................................................................
sensor voltage 105
Smoothing .................................................................................................................................................. 106
Min/max volumes
.................................................................................................................................................. 108
Quantities .................................................................................................................................................. 109
Calibration .................................................................................................................................................. 110
Correction .................................................................................................................................................. 111
Creation ......................................................................................................................................................... 112
Deletion ......................................................................................................................................................... 113
2 Pathologic
...................................................................................................................................
flags 114
Presentation
......................................................................................................................................................... 114
Creation ......................................................................................................................................................... 115
Modification
......................................................................................................................................................... 119
Deletion ......................................................................................................................................................... 122
3 Technical
...................................................................................................................................
functions 123
4 Stand-by
................................................................................................................................... 124
5 Password
................................................................................................................................... 124
Part VIII Appendix 128

1 Format
...................................................................................................................................
connection series 128
General structure
......................................................................................................................................................... 128
Detailed format
......................................................................................................................................................... 130
Configuration
......................................................................................................................................................... 134
Exporting the
.........................................................................................................................................................
data 136
Connection
.........................................................................................................................................................
Cable 138
2 Long
...................................................................................................................................
lasting stop 139
3 Notes
................................................................................................................................... 140

Introduction

6 MS9-5 Operator manual Introduction
1 Introduction
1.1 Setting up advice
1) Placement
Choose a suitable place to set up the machine.

- Place it on a flat, solid surface to ensure a good ventilation of the instrument .
- The left side must be clear for the reagents pack.
- The right side must be clear to open the CD-ROM drive/recorder drawer.
- The apparatus and the reagents pack should be used in a place at room
temperature and not in front of sunny windows.
2) Electric connection
Electric connection should be with an earth point. A good earth connection is

imperative to assure the apparatus's good running.
An intermittent or bad earth contact may lead to incorrect analysis.
Electric Current :
110-220V / 50-60 HZ 300VA
Automatic switch commutation.
Fuses :
F 2A-250V for 200-240V
F 4A-125V for 100-120V
An earth plug must be used for the user and apparatus's elements safety.
Any deterioration in the apparatus's elements due to being connected to a non-
earthed electric current will render the guarantee null and void.
1.2 Switching on
Before plugging in the apparatus, you are strongly advised to read and
follow the instructions for the electrical and reagents pack connections found in
the MS9-5 Installation Manual.
When you switch on the apparatus, all the starting up, testing, initialisation and
priming operations are automatic.
There is an option to invalidate the priming at the start, but you are recommended
not to use it.
The apparatus has been constructed to remain plugged in 24 hours a day. It is
capable of putting itself automatically into sleep mode during the evening and
start again in the morning at a programmed time so as to be available quickly.

Introduction MS9-5 Operator manual 7
In this chapter we are going to describe the standard procedure from starting as
well as the different initialisation and test displays to the final display screen.
1.2.1 Plugging in
When all the electric and fluidics (reagents pack) connections have been carried
out, check you have well installed the MS card in its drive on the left of the
machine with the serigraphy facing upwards. Then switch the apparatus on.
The switch is at the back of the apparatus, on the left :
1.2.2 Initialisation of the system
Starting the system is carried out in several steps :
- Loading the Windows running system.

- Automatic starting of the program.
- Auto-tests of the system's components.
- Priming the reagents pack and decontamination 'blank'.
The program and apparatus's auto-test are launched when the ' System
initialisation' dialogue appears.
1) Language
The version's language is found in the 'Language' zone.
The apparatus is configured in the destined country's language during its

preparation. You can contact the technical department to know which languages
are available.
2) System
The setting system displays the results of the different tests carried out during the
starting up :
- Detection of the system's different elements.
- Model display.
- Display of the fluidic and acquired programs.
- Final status of the machine.
If all the electronic and mechanical tests have been passed successfully, 'OK'
should appear on the bottom, then, after a few seconds, the starting up display
should give way to the main display.
In case of a problem, if the auto-test procedure detects a mechanical or

electronic fault, an 'Quit' button will appear at the bottom of the starting up
display.
Before contacting the technical department, quit the program by clicking on this
button, then quit Windows. Switch off the apparatus and wait a few seconds
before switching on again.
If the problem repeats itself, note the information displayed in the starting up
dialogue before contacting the technical department :
- Program version
- If the problem is at the fluidic and/or acquisition level.

In our case :
Program Version : 1.2 build 7
Acquisition : Problem (Obviously the problem comes from the acquisition card).
3) Priming
If the machine has been parameterised to carry out a priming during its starting
up (the 'Priming' box should appear checked), the apparatus will carry out several
additional technical operations :
a) Priming the Hemoref
b) Cleaning the needle with Hemoref
c) Priming the reagents pack

d) Decontamination counting cycle (if the option has been selected in the
parameter system panel)
When the apparatus is ready for use the main display will appear like this :
The word 'Ready' appears in the status line at the bottom.

1.2.3 Main display
The MS9 main display is made up of several distinct zones marked out with
numbers :
1) Menu Zone
This zone is at the top of MS9-5's main window.

It corresponds to a Windows program's classic menu with the same mouse and
keyboard use rules.
The apparatus handles keyboard shortcuts.

Ex : Alt + 'S' to access the 'Settings' menu.
2) Toolbar and bank selection
The tool bar works as a shortcut for the program's most used functions.
Ex : a click with the mouse on the first icon from the left on the tool bar,
symbolised by a tube of blood, starts an analysis.
For a description of each icon's function, place the mouse's cursor over it. After a

few seconds the function's description is displayed.

The last element is a roll-down list enabling you to select items from the current
bank.
3) Identification zones
An identification zone consists of three elements :

- A button to display the complete identification panel.
- A text zone in which is displayed the patient's name and identification number.
- A last text zone displaying the bank used.
There are three identification zones :

- Zone 1, on the top left, is the identification zone for the displayed analysis.
- Zone 2, on the bottom left, is the identification zone for the analysis in progress.
- Zone 3, on the top right, is the identification zone for the next analysis.
4) Result zones

The result zone is made up of three groups :
- Zone 1 : white blood cells group.

- Zone 2 : red blood cells group.
- Zone 3 : platelet group.
Color, character and its size as well as the displayed units are programmable
(refer to the 'Parameters' chapter further on in this manual).
5) Graph zones
The curve display zone is made up of 4 graphs in 5 DIFF mode and 3 graphs in 3
DIFF mode :
5 DIFF mode :
- Scattergram of white blood cells (WBC) .
- Histogram of eosinophils (Eo).
- Histogram of platelets (PLT or THR).
- Histogram of red blood cells (RBC).
3 DIFF mode
- Histogram of white blood cells.
- Histogram of platelets.
- Histogram of red blood cells.

5 DIFF mode Graphs 3 DIFF mode Graphs
6) Monitoring count zones
Above the top graphs and under the bottom graphs are two zones displaying a
real time monitoring of the most important parameters during the count :
- The probe voltage of soundings of the WBC, eosinophils and RBC/platelet
tanks.
- The count drop.
All the abnormalities, blockages and air leaks, are immediately translated by the
modification of one or several monitored curves.
7) Status bar
Finally, the status bar at the bottom of the display is made up of 6 zones :
Beginning on the left :

- Date and time.
- Machine status : ready, in sleep mode, etc.
- Identification mode.
- analysis counter of the remaining running MS-Card.
- The machine's use mode (3 DIFF or 5 DIFF).

- Icon for detecting the presence of the tube of blood or memorising the automatic
starting for the next analysis.

Analysis

18 MS9-5 Operator manual Analysis
2 Analysis
2.1 Carrying out an analysis
2.1.1 Testing the blood sample
1) Anti-coagulant
The most frequently used anticoagulant is the EDTA K3 or sometimes the EDTA
K2. The optimal concentration is from 2 to 3 mg of EDTA per ml of blood.
Other anticoagulants, such as Heparin, can generate instable results. On very
delicate thrombocyte pathology, 'Citrate' can be used.
2) Blood sample tubes
Nearly every types of sampling tubes can be used with capacities from 1 to 5 ml.
By default the MS9-5 uses from 40 to 80 microliters of whole blood to complete
an analysis.
The quantity used by MS9-5 is programmable and depends on the mode used :
3DIFF or 5DIFF.
For lower quantities (particular cases such as new-born for example), a special
blood sampling kit meant for blood micro-sampling is also available :
HEMO-KIT 200 Reference : 3MSR0550.
You are recommended, and it is crucial, to always fill the tube used with the
anticipated quantity of blood. Do not half fill it.
For example, a 5 ml tube should be filled with 5 ml.
3) Blood sample
It is recommended to not take a blood sample from a patient under anaesthetic.

The MS9-5 analyzer uses analgesic derived doses to carry out the leucocytes
differentiation. A denaturation of the balance of this kind of agent may lead to an
instability of the leucocytes formula.
To perfect the anti-coagulant's effect, it is important to carry out a
homogenisation of the tube several times just after having taken the blood
sample. This will greatly remove the likelihood of the presence of any micro-clots.
4) homogenisation
Before carrying out an analysis on the apparatus, it is important to perfectly

homogenise the tube. The mixture being in function with the sedimentation state
of the tube. A mixture of up to one or two minutes is sometimes necessary.
It is essential to carry out a strong and long homogenisation if :

- The blood has just been taken (from 5 to 30 minutes).

Analysis MS9-5 Operator manual 19
- The blood is sedimented.
2.1.2 Preparation
Two very important points must always be checked before carrying out an
analysis :
- The selected bank : male, female, new born, other...

- The mode used : 5 DIFF or 3 DIFF.
1) Bank Selection
Look at the 'Bank' chapter in this manual for a full description of the banks.
The present bank appears at the end of the tool bar of the main window (refer to
the main display description in the 'Switching on' chapter).
The mode used is displayed in the status bar at the bottom of the display : 5 DIFF
or 3 DIFF.
To change banks, for example from male bank to female bank, select the
required bank from the roll-down list in the tool bar in the following way :
Click with the mouse on the arrow of the roll-down list :
Select the required bank :
To finally display :
There are two cases that we point out further on in this manual that do not
take into account the bank currently selected :
1) If the identification mode is 'work list', the bank used will be that programmed
in the notebook and not that displayed.
2) During the quality control, the bank used is the 'CONTROL' bank.
2) Possible change of mode

To change the apparatus's use mode from 5 DIFF to 3 DIFF or vice versa, click
with the mouse on the 'Settings' menu, then select 'System' or press the 'F12'
function key, a dialogue window similar to the display below appears.
Use the 'Parameters' box on the top right of the tab system to select the count
mode (3DIFF or 5DIFF).
Then click on the 'Ok' button to save the change.
For a full description of the 'System setup' dialogue, refer to the 'Parameters'
chapter in this manual.
3) Blood homogenisation
Shake the tube well up and down several times.
It is essential to carry out a strong and long homogenisation if :

- The blood has just been taken (from 5 to 10 minutes).
- The blood is sedimented.
4) Check the tube's adapter
Each kind of tube has a specific adapter.

2.1.3 Analysis identification
To identify the next analysis, press the 'F4' function key or click on the
'Identification' menu and select 'Patient identification', or yet again click on the
next analysis's identification zone button (refer to the main display's description in
the 'Switching on' chapter).
You can key in the analysis's identification at different times :
- When preparing a tube series :

When keying in the identification of the tubes in the work list (refer to the
'Identification' chapter further on in this manual).
- Just before the analysis :

As in our present case.
- During :
For that, click on the 'in progress identification zone' button (on the bottom left).
- After :
By clicking on the displayed analysis identification zone button (on the right top).
Changing the analysis's identification number and the used bank is not allowed in
this case.
A quick description of the dialogue window :

- The 'Mode' zone displays the chosen identification mode.

- The 'Bank' zone displays the current bank. To change it, click on the roll-down
list.
- The 'Identification' zone enables you to key in the file number.
In our case, the 'Number' zone in the 'Identification' box has already been given
the information. The identification number is generated automatically as the
identification mode is displayed 'Auto date' in the 'Mode' box on the top right.
This mode automatically generates a file number in function with the date of the
day and the number of analyses carried out.
(Refer to the 'Identification' chapter in this manual for a full description of all the
existing modes).
- A 'Patient' zone for keying in the patient's name and first name.
Click on the 'Save' button to close.
NB. The bank choice can only be carried out before starting an analysis. It is
impossible to change banks during the analysis.

2.1.4 Starting an analysis
Several precautions must be taken with the tube of blood to carry out a good
analysis.
1) Preparation of the tube
Shake the tube up and down several times.

Gently remove the stopper from the tube.
2) Placing the tube and starting the analysis
Place the tube in the adapter by pressing downwards. This automatically starts
the analysis.
There are other methods for starting an analysis. If the tube is already in place,
you can :
- Click on the 'Tube of Blood' icon in the tool bar.
- Press the following key combination : Control (Ctrl) + key 'C'

- or click 'Analysis' and select 'Run'
It is possible to deactivate the automatic starting by pressing the tube in the stand
('Fluidics Parameters' Chapter).
3) Blood test(s)
When the analysis starts, the apparatus begins by carrying out one or two blood
tests according to whether it has been configured in 3DIFF or 5DIFF mode.
Do not try to remove the tube during the blood-testing phase.

Especially in 5 DIFF mode. Wait until the second test has finished before
removing the tube.
4) Removing the tube of blood
After the second test, the apparatus gives off an audible signal so that you can
remove the tube of blood from the stand.
(The 3 DIFF mode gives off a sound after the sole test).

5) Putting the stopper back into the tube
Put the stopper well back into the tube of blood.
2.1.5 Analysis cycle
This paragraph's aim is not to give a detailed description of the dilution cycle but
to give a summary.
For further details on the apparatus's full dilution cycle operation, refer to the
reference manual.
The analysis consists of several phases :
1) Taking the blood test
We described this previously.
2) Dilution preparation
a) In the 5 DIFF mode, the first blood test is for dilution in the eosinos chamber.
The second blood test is for the pre-dilution chamber.
b) In the 3 DIFF mode, the sole blood test goes straight to the pre-dilution
chamber, followed identically as in the 5 DIFF mode.
3) Counting
The counting itself is divided into four equal parts depending on the length of time
programmed in the bank. This detects any possible homogeneity or micro-clot
problems by comparing the intermediary results.
The program also carries out a real time probe voltage and counting depression
test to detect any possible blockages in the orifice of one of the measuring
chambers.
The accepted tolerance for probe voltage and count drop variants are
configurable and are carried out on the dotted line graphs.
4) Displaying and saving the results
Once the count is finished, the apparatus calculates, displays and then saves the
results.

5) Rinsing out the machine and printing
The analytic cycle finishes with a diluter rinsing and if needs be, printing and
transfer via the apparatus's serial port of the hematological test. These
functionalities are configurable and will be tackled further on in this manual.

2.1.6 Next analysis
It is possible to program the next analysis during an analytic cycle by placing a

new tube of blood in the stand and pressing it downwards. An audible signal will
be heard and a small tube of blood is displayed on the right of the status bar to
confirm the memorising of the next analysis.
It is possible to cancel the analysis programmed by pressing the tube again in its
stand. The little tube of blood is displayed in the status bar then disappears.
To key in the identification information, click on the identification zone button for
the next analysis (refer to the main display's description in the introduction
chapter) to open the next analysis's identification dialogue box.

The information concerning this analysis must imperatively be saved before

the end of the current analysis, otherwise the keyed data will be lost.
2.2 Consulting an analysis

2.2.1 Recalling the day’s analysis
To recall the day's analyses, use the combination of the keys 'Ctrl' + 'R' or even
click on the 'analysis' menu and select 'Load'.
You may also use the following tool bar shortcut :

A dialogue box 'analysis search' is displayed as below.
By default options are configured to recall the day's analyses. Just click on the
'Search' button on the top left for them to be displayed.

The number of analysis is displayed on the bottom left.
If an alarm went off during an analysis, an exclamation mark is displayed in

the 'Status' column.
To consult a particular analysis, select it in the list by clicking on the line

concerned, then click the 'Load' button.

The default display order works backwards, that is to say the first line will display
the most recent analysis.
2.2.2 Search engine
The search engine is multicriteria.

The dialogue box consists of several zones :

1) A date zone
To use the analysis search by a date the displayed date must be checked :
To change the date, click with the arrow on the right of the date to display a
calendar, then browse in this to select the sought after date.
To choose the period, select one of the three following comparison operators :

'=' : Search the analysis carried out on the selected date.

'>=' Search the analysis carried out since the selected date.
'<=' Search the analysis carried out before the selected date.
If the date is not checked, the search is carried out by ignoring the displayed date
and the comparison operator.
Examples :
The following selection searches for all the analysis carried out on 7/02/2003.
This displays all the analysis carried out since 7/02/2003.
2) A zone for additional criteria
To carry out a more precise search, several additional criteria may be specified.
a) Searching with the name
A 'Name' zone lets you carry out a search by the patient's name.
The search motor can take the '%' substitution character.
Ex : DU% in the 'Name' zone enables a search of all the analysis of the patients
whose name begins 'DU'.

You can combine this criteria with one requirement on the date by checking it.
b) Searching with a identification number
Using the file criteria is similar to that of the name. Enter the complete
identification number or only a part of it with a substitution character.
In the following example, the search concerns all the analysis carried out in 2003
with the identification number ending with '009'

c) Searching by bank, operator, department or division
These four fields respectively specify a precise bank, a particular operator, a

department and/or a particular division.
All these fields may be combined for a multicriteria search.
To suppress a criterion, just erase the contents of its field. To do that, select its
contents with the mouse then erase it by using the 'Del' key on the keyboard.
3) Limiting the number of analyses during a search
Just above the 'Search' button is a 'Max' counter.

The counter value limits the maximum number of analysis during a basic search.
The by default number is fixed at 100. It is not advisable to use a too high a
maximum value that, on one side over-charges the apparatus's memory, and on
the other side will appreciably slow down the multicriteria search.
4) Remarks
a) Browsing amongst the found analysis.

There is a means of quickly consulting the result of a search without coming back
to the dialogue box.
When the result of a search consists of several analysis, the 'up' and 'down'
arrows are activated in the tool bar.

Clicking on these arrows displays the previous and following analysis.
b) Printing a selection of analysis.

Click on the 'Print' button to edit the result you asked for.
The results are printed in chart form.
c) Multicriteria
It is possible to combine several criteria including the date :
Example :
It is possible to display all the analysis carried out by one given operator, one
precise bank or since a given date.
2.3 Exporting
2.3.1 Exporting the day’s analysis
To export the day's analysis, click on the 'Analysis' menu and select 'Export'.
Or even use the following tool bar shortcut :
A dialogue box 'analysis search' is displayed as below.

The by default options are configured to export the day's analysis. Just click on
the 'Search' button on the top left to display the list of analysis carried out during
that day.
Then 'Export' button to choose between the communication serial port and a text
file.

Then the 'Ok' button.
Remarks :
1) The choice of serial ports and its configuration, delimited character field and
the decimal symbol are configurable. For more information, refer to the
'parameters' chapter further on in this manual.
2) In the case of an export in a text file, the name and destination of the export
file must be specified.
The kind of exported file is a table that contains as many lines as analysis. The
character that separates the fields is by default ';' (Semi-colon). This kind of file
is easily exportable in an 'Excel' or other kind of spreadsheet program.
3) The format of the sent data on the serial port is described in the notes at the
end of this manual.
2.3.2 Search engine
Using the search engine in the 'Export' dialogue box is identical to that of the
analysis consultation.
Refer to the 'Search engine' paragraph in the 'Consulting an analysis' chapter for
a full description of its use.


Identification

40 MS9-5 Operator manual Identification
3 Identification
3.1 Patient
Identification enables keying in information concerning the analysis and the

patient. You can have access to it in several ways :
- 'Identification' menu then select 'Patient identification'.
- By the 'F4' function key.

- By the button on the left of each identification zone (refer to the description of
the main display in the 'Switching on' chapter).
The dialogue is displayed in this way :
1) 'Analysis' zone
Selects the analysis for which you wish to key in identification information.

Identification MS9-5 Operator manual 41
There are three possible choices :

- Displayed analysis
- Current analysis
- Next analysis.
Depending on the apparatus's status, these options are not always available. If
no analysis is in progress, for example, this option will be invalidated.
It is the same with the type of analysis. Some options will not be available : it is
impossible to change the bank for a current or a finished analysis.
2) 'Identification' zone
This zone keys in or changes the analysis's file number.

Changing its contents depends on the analysis's status and the identification
mode used. This mode is displayed in the 'Mode' box. Use and choice of the
identification mode is described in the 'Parameter – System' chapter.
3) 'Patient' zone
The two zones in this box enable the keying of the patient's name and first name.
4) 'Bank' zone
The bank box indicates the bank used for the analysis.
The bank can only be changed for the next analysis.
5) 'Operator' zone
Enables keying the operator's name. The 'New' button gives direct access to a
new operator's keying dialogue.
6) 'Origin' zone
For a hospital environment use of this apparatus, the 'Department' and 'Division'
fields enable the entry of additional information on the department to which the
patient belongs.
7) 'Comments' zone
This zone is for entering observations related to the patient or the analysis's
results. This zone will be printed.

3.2 Work list
The work list is a particular instruction mode for the apparatus to key the
information for the machine to carry out the analyses in advance and in a
predefined order.
This chapter describes the way to key in or edit the work list. The instructions on
how to use it are referred to in the 'Parameters' chapter further on in this manual.
To access the work list's editing dialogue, tap the 'F2' function key or click on the
'Identification' menu then select 'Patient work list'.
An empty dialogue box is displayed if no work list has been keyed beforehand :
To key an analysis's information, click on the 'Add' button.

Complete the information as indicated previously in the 'Patient identification'

rubric, then click on the 'Save' button.
A new line is displayed :

The work list will be filled in as the analysis are keyed.

To modify a line, select it by clicking on it, then use the 'Edit' button.
Do the same thing to delete with the 'Delete' button.
The 'Delete all' button deletes all the analysis in the work list in one go.
The key order will be the analysis's passage order.
The work list may be modified or completed later as the data is saved.
To key a new work list, begin by erasing the present analysis with the 'Delete all'
button.
The 'Status' field is updated as the work list is filled.

3.3 Operator
The program enables a predefined list of operators to be keyed in. To do that,

click the 'Identification' menu and select 'Operator identification'.
With the displayed dialogue box, you can enter a new operator or modify the
name of an existing one.

All the added operators' names will be displayed in the 'Operator' box of the
'Patient identification' dialogue.
3.4 Department and division
In a hospital environment use, the apparatus enables the keying in of the

department and/or the division that the patient belongs to. The 'Department' and
'Division' menus enable the list of existing departments and divisions to be
created in advance.
A dialogue box gives you the opportunity of keying in a new department,
modifying it and deleting an existing one.
The divisions are linked to one department. Remember to access a precise

division, you must select the right department in the roll down list.

All the added department and division names will be displayed in the 'Origin' box
of the 'Patient identification' dialogue box.

Calibration

50 MS9-5 Operator manual Calibration
4 Calibration
4.1 Presentation
There are several kinds of calibration :

Factory calibration : references the apparatus from every angle. This value
table is fixed during the machine tests whilst in the factory.
Operator calibration : enables the user to carry out his own calibration.
Prediluted calibration : is a particular calibration used in the case of a prediluted
rather than whole blood use. By default, all the coefficients are 1.
Sampler calibration : is an additional calibration adjusting the results during the
use of the tube's automatic sampler.
Probe voltage calibration : The apparatus carries out a constant follow-up
before and during the applied electric field's counting in the measurement
chambers. This following-up enables the immediate detecting of any possible
blockage. To define the reference's optimal field value, a calibration needs to be
carried out when it is installed, during a counter orifice change, a probe voltage
value or following any electronic modification.
You must be on 5DIFF mode to calibrate the apparatus.
4.2 Manual calibration
The manual calibration consists of carrying out a blood analysis of which its
parameter value is known in advance, then to calculate the value of the
coefficients provided by the machine the results must be applied to find the
targeted values.
The calculation is carried out according to the following rule :
Former coefficient x Target value

New coefficient = ------------------------------------------------
Measured value
Think of selecting the appropriate bank to carry out your calibration.

In particular, choose the 'CONTROL' bank for a calibrated blood test.
This bank has been specially configured for this type of blood.
To key the coefficient, click on the 'Calibration' menu then select 'Coefficients'.

Calibration MS9-5 Operator manual 51
Click the 'Ok' button to save the keyed values.
There are two methods for verifying the keyed value's accuracy :
- Carrying out a new analysis with the same blood.
- Directly recalculate the previous analysis's results taking into account the
calibration's new coefficient. To do this, click the 'Calibration' menu then select
'Recalculation'.
Check the validity of your calibration by comparing the displayed results to your
targeted values.
4.3 Automatic
The apparatus proposes a simple automatic calibration mode that takes the
manual calibration mode's logic previously described but carries out the
operations automatically.
To carry out an automatic calibration, click the 'Calibration' menu the select
'Coefficients'.
Click the 'Automatic calibration' button.

Select the target blood in the list. (Refer to the 'Target values' paragraph to
create a target values lot).
Now we have selected our target values lot, validate by clicking the 'Ok' button.

Place your tube of test blood on the stand and click the 'Analyse' button.
When the analysis is finished, the program displays the analysis's results and
calculates the coefficient.
All the coefficients are selected by default but you may choose to only keep those
of your choice by removing the ticks on the unwanted parameters.
A warning is displayed if the expiry date is past.
To apply the new coefficients, save the values by clicking the 'Save' button.
There are two methods of checking the validity of the new coefficients :

- Carrying out a new analysis with the same blood.

- Recalculating the previous results taking into account your new calibration
coefficients by clicking the 'Calibration' menu then 'Recalculation'.
4.4 Target Values
Click the 'New' button. Key the lot's reference, its level and expiry date.
To key the target values of this new lot, select it in the list then click on 'Change'.
To key or modify a target value, select the required parameter then the 'Change'
button :

All you have to do is to key the target value and the variations interval. The high
and low values are calculated automatically.
4.5 Probe voltage
The apparatus carries out a continuous follow-up, during the counting and of the
applied electronic field in the measurement chamber. This follow-up enables an
immediate detection of any possible blockage. A calibration is necessary to
define the reference's optimal field values, when it is installed, during the counter

orifice's change, a change in the probe voltage's values or after any electronic
modification.
To carry out a probe voltage calibration, you must make sure that the
counter's orifices are perfectly clean. To do this and in case of doubt, a WBC,
RBC and Eos orifice technical function cleaning may be necessary.
'Calibration' Menu, then select 'Probe voltage'.
The dialogue box contains a 'Old values' box that displays the current machine
values and the empty 'New values' box.
To start up a new calibration, click on the 'Calibrate' button.
The apparatus carries out an analysis then completes the 'new values' box with
the calibration values established during the analysis.
To accept these new values click on the 'Save' button.

The values shown in the previous panels are not entirely the reference
values and are only used as an example.

Quality control

60 MS9-5 Operator manual Quality control
5 Quality control
5.1 Presentation
Quality control carries out regular tests on control bloods to verify the apparatus's
calibration.
The 'Targeted values' menu enables keying the targeted values and the
acceptable variations of your control bloods.
The quality control is carried out in three parts :
1) Keying or editing the targeted values for the used control bloods.
2) analysis from a given control blood.
3) Consultation and editing.
The results can be exported under an ASCII table form for later specific
treatment.
5.2 Target values
In the 'Quality control' menu, select 'Target values'.
The 'New' or 'Read' buttons enable to create a lot of target values either manually
or automatically.
The 'Change' button enables editing of the existing lot.

Quality control MS9-5 Operator manual 61
A control blood is often associated with several pieces of important information :

- Its level : low, normal or high.
- Its lot number.
- Its limited period date.
- Name of the parameters.
- Targeted values.
- Acceptability interval.
There are two methods for entering the information :
1) Manual mode
This method consists of creating a lot reference for each level, then keying the
target value and acceptable range for each parameter.
To create a lot manually, click on the 'New' button.
Fill in the zones, references, control blood level and its expiration date. Click on
the 'Save' button to save the values. The new reference will be displayed in the
list from then on.
It is now a matter of keying the target values and the acceptable ranges. To do
this, select the lot and click on the 'Ok' button.

An empty parameter chart is displayed with the previously provided

characteristics :
To key the values, select a line and click the 'Edit' button :

Enter the target value and the provided delta with the control lot.
The high and low limits adjust automatically in function with the keyed values.
2) Automatic mode
To use the automatic mode, you need the smart card that goes with your test.
(Reference 102 CONTROL-DIFF). All the lot creation operations and the keying
of the target values and ranges are automatic.
Click the 'Load' button :
Replace the MS-CARD with your control blood data card.

Click the 'Reading' button. In a few seconds the three lots of the low, normal and
high levels will be created with their associated values.
Replace the MS-CARD in the apparatus before carrying out a new analysis.

5.3 Analysis
A quality control analysis is carried out after having selected the lot used.
The bank selected is of the 'Control bank' kind.

Then click the 'analysis' button.
A recapitulative dialogue verifies all the elements before starting off a test
analysis.
It is recommended to check the lot's level before starting off an analysis.

It is essential and fundamental that the test blood is at room temperature before
mixing.
A cold homogenisation can greatly damage the test blood's quality through
hemolysis.

5.4 Consultation
Consulting the results of the analysis carried out for a quality control can be done
in two ways :
- A listing mode in the form of a result chart for a given period.
- A Levey-Jennings type graph for the same period.
To have access to the quality control's consultation dialogue, select 'Consultation'
in the 'Quality control' menu.
1) Result chart
This dialogue displays an analysis by line and an additional column for each
parameter in which the difference, in percentage to the target value, is displayed.
You can choose the level from a roll-down list on the top left.
The period is fixed, by default, to two months and a maximum number of 50
analysis displayed.
To have a result chart for different criteria, use the 'Search' button.
The results can be printed in chart form or exported in ASCII file form that can
easily be imported into an 'Excel' or other chart type.
The 'Load' button enables the consultation of one particular analysis.

2) Graphs
To have a graphical representation of the results, click on the 'Graph' button. To

go back to the text mode, click on 'List'.
The search criteria remain on the top of the dialogue, but the chart is replaced by
a graph.
To select a different parameter, use the roll-down list on the right.
The white dotted line is the graph's reference and corresponds to the parameter's
keyed target value.
The yellow dotted line represents this parameter's measured values' average. Its
relative position to the target value will immediately give indications on the
apparatus's drift and on the necessity of carrying out or not a new calibration.
The green dotted lines correspond to the acceptability distance.
The red dotted lines correspond to twice this distance.
The top and the bottom of the graph correspond to three times this acceptability
value.

Parameters

68 MS9-5 Operator manual Parameters
6 Parameters
6.1 Presentation
The apparatus has numerous and varied parameters. They are grouped in
various categories :
1) Display format
This dialogue groups all the screen display parameters :
- background color used.
- fonts.
- graph colors.
- column position, etc.
2) Printing format
This dialogue groups all the parameters for printing the results :
- Headed contents.
- Color option.
- Fonts used.
Option for graph printing, etc.
3) Fluidics
This dialogue groups all the apparatus's fluidics configuration.
4) System
This dialogue groups all the machine's systems together :
- Serial port configuration.
- Choice of identification mode.
- Settings for sleep mode and automatic starting up, etc. .
5) Units
This dialogue groups all the apparatus's units configuration.
- Unit system choice.
- Language choice in which the results' wording will be displayed.
- Modification of the wording of a parameter etc.
Changing some of these parameters may lead to incorrect displaying of the

results or the machine's non-functioning.
All modification must be carried out by a competent person.
6.2 Display formats
To have access to the 'Display parameters' dialogue, click on the 'Parameters'

menu, then select 'Display format' or tap on the 'F8' function key.

Parameters MS9-5 Operator manual 69
This dialogue box has two tabs :
- a 'Text format' tab for defining the results zone.

- a 'Graph format' tab for defining the graph zone.
1) Text format
a) 'Labels' box
This box enables you to change the color of the text of the three groups of the
results : WBC, RBC and PLT.
To change the color of the text, click on one of the 'Change' buttons and select
the color of your choice.

b) 'Background' box
Enables you to change the background color of the text zones of the three groups
of results : WBC, RBC and PLT.
Do not use the same color to display the text and the window background.
The results will be illegible.

c) 'Channel' box
Enables you to select the display of a group of parameters and to test the choice
of colors before validating them.
d) 'Exponent' box
This option enables you to choose the exponent display type (normal or scientific
notation).
e) 'Border style' box

To change the visual effect of the windows of the main display (with or without a
frame, embossed or not).
f) 'Results' box
The 'Null forced' option 'forces' all the results to a no value when doing a blank
(i.e. an empty analysis).
It is advisable to tick this option.
g) 'Font' box
To change the font used when displaying the results. 'Change' button, then select
the required font and the size.
Do not choose a too big a size, otherwise the display lines will overlap.
h) 'Results position' box

This box proposes position adjustment options for the 2 results columns.
Each group of results is divided into 2 sub-groups named here 'Column 1', for the
left column and 'Column 2' for the right one.
You can move the position of the parameters horizontally for each column with
the cursor.
The first cursor, 'Label', changes the horizontal position of the wording of the
parameters.
The 'Alarm' cursor positions the possible alarms.
The 'Value' and 'Unit' cursors position the display of the results and their units.
2) Graphics format
This dialogue modifies the graphs' color and appearance.

The 'Graphics color', 'Graphics backcolor' and 'Graphics fillcolor' boxes change
the colors of the graphs.
The 'Border style' box changes the appearance of the graphs' frame.
Checking the box 'Histogram amplification' enlarges the size of the histogram.
The 'Graphics line width' field modifies the line's size.
6.3 Printing formats
To access the printing parametering dialogue, click on the 'Parameters' menu

then select 'Printing formats' or tap directly on the 'F9' function key.
This option is only authorised if a printer is installed. To install and connect

a printer, refer to the document provided with the printer.
This dialogue has four active tabs :

- One 'General' tab for the most up to date options.
- One 'Header' tab for the headed parametering.
- One 'Graphics' tab to parameter the graphs' colors and aspects.
- One 'Text' tab to modify the font used and positioning the results.

1) 'General' tab
a) 'Automatic edition' option.

If this option is validated, printing will be carried out automatically at the end of
each analysis.
b) 'Color' option
For printing the graphs and alarms in colors if the printer is able to.
c) 'Graph printing' option

For printing the curves beside the results.
d) 'Border', 'Background' and 'Header' options

The 'Header' option prints the information keyed into the 'Header' tab at the top of
the sheet of paper.
This option may be invalidated for the use of headed paper.
If headed paper is used, you can stipulate the number of lines to be jumped at the
beginning of the printing.
The 'Background' and 'Frame' options enable you to include a grey background
and a frame around the heading.
2) 'Heading' tab

If you wish to personalise your printing, fill in the different zones.

The 'Headed printing' option of the 'General' tab must be selected.
3) 'Graph' tab
The different parameters proposed enable you to change the color of your
graphs.

To change one of the colors, click on the corresponding 'Change' button.
The 'Ok' button to validate the choice.
4) 'Text' tab
To change the fonts and their size used during printing, click the 'Change' button

in the 'Fonts' box.

The results' position may be adjusted in function to the type and size of the fonts
used. To do that, move the cursors horizontally.
6.4 Fluidics
The fluidics dialogue (or diluter's configuration) modifies a certain number of the
apparatus's fluidics parameters...
For fuller information, refer to the 'Dilution cycle data' chapter in the reference
manual.
The values displayed above are not necessarily the reference values of your
machine.
Every fluidics parameter modification must be carried out by a qualified person.
An erroneous value may cause serious machine malfunctioning.
If a modification has to be carried out in spite of everything, you are strongly

advised to save the current parameters by clicking the 'Save default values'
button beforehand.
You may recall these values later by clicking on the 'Load default values' button.
1) 'Syringe' tab

To modify the motor's parametering that commands the syringe step by step
(speeds and movements)
2) 'Needle' tab
To steer the vertical and horizontal movements of the blood testing needle.

3) 'Delay' tab
For parametering the fluidics time delays.

4) 'Others' tab
Various parameters linked to the apparatus's compressor.
5) 'Sensors' tab
The apparatus has a set of two types of sensors :

a) Positioning sensors to control the needle and syringe's movements.
b) Liquid detection sensors to control the presence or absence of relatives.
The 'Status' box enables the activating or deactivating of one particular sensor. If
the sensor's name is checked, it is activated.
The 'Sensitivity' box regulates the captor's sensitivity if it is in service.
6.5 System
To have access to the 'Configuration system' dialogue, click on the 'Services'

menu then select 'System' or tap directly on the 'F12' function key.

This dialogue has twelve tabs :
- 'System' to regulate the date and time, sleep mode, etc.

- 'Hb' to regulate the hemoglobin measurement.
- 'Timers' to regulate the program's execution priority and internal tempo.
- 'Sensitivity' to determine the acceptable variations of the counting voltage.
- 'Serial configuration' for all the RS232 communication adjustments.
- 'Parameters' to define the list of the displayed parameters.
- 'Counting' to define the apparatus's reference values.
- 'View' for future functionalities.
- 'Identification' to identify and parameter the different modes of identification.
- 'Blank' to parameter the contamination alarms.
- 'Password' to change the machine's passwords.
- 'Export' to parameter the field separation characters and the decimal symbol
during a result exportation.
Some parameters must only be modified by a qualified person. An

erroneous value may cause apparatus malfunctioning. As a precaution, save the
current parameters before any modification by clicking the 'Save default values'

button. You can thus, in case of problems, restore the apparatus's initial
adjustments by clicking the 'Load default values' button.
6.5.1 Tab system
The first group of parameters on the top left contain the time and date system
adjustments, the stand-by time and the start-up time.
Time system = Windows time
Date system = Windows date
Stand-by hours and automatic starting

up.
Day(s) without starting up

automatically.
The 'Default bank' enables you to choose the bank selected for starting up.
The 'Parameters' group enable you to change the 5DIFF use mode to 3DIFF or
vice versa.
The eosinos chamber cannot be used in 3DIFF mode. The 3DIFF mode results
are compensated by an adjustable coefficient for that. This coefficient is 98% by
default. It is one of the reasons why you should calibrate the apparatus in 5DIFF
mode.
The 'Priming' group configures a starting up with or without a priming as well as

its type.
A decontamination pre-counting valid option only when the programmed priming
is able to do an 'empty' decontamination numeration, just after the priming
procedure. If a contamination alarm takes place, you are advised to carry out
several 'blanks' or clean the chambers (refer to the 'Technical services' chapter).

The last group 'Stand-by' enables you to validate or invalidate the automatic
stand-by mode action.
It is essential to carry out a putting on to stand-by mode every evening and

a long pre-counting priming before every use.
6.5.2 Hb tab
The apparatus carries out several measurements and sends back an average
value to determine the hemoglobin value.
The following zones are to set the number of measurements carried out to
measure the hemoglobin and hemoglobin 'blank'.
The final value being the difference between the average of the measured values
and the 'blank' value.
To assure the measurement's validity, a verification is carried out on the operated

current. If the measured values are outside the programmed values, the
apparatus sends back an error on the hemoglobin parameter.

The above used values are not reference values.
6.5.3 Sensitivity
This tab enables you to configure the apparatus's sensitivity for possible
blockages or homogeneity problems.
1) Description of the parameters in the right column.
Delta acceptable in relation to the probe

voltage value asked for.
Maximum acceptable variation of the probe

voltage during the count.
The apparatus carries out four successive

counts. The 'delta debitmeter' value,
expressed in %, is the maximum acceptable
variation between the found results. A
homogeneity alarm is sent back for the
given means if to the contrary.

2) Description of the parameters in the left column
The calibration values are normally set

during the probe voltage's calibration.
(Refer to the 'Calibration 'chapter).
The 'Spoiling delta' parameters, are the

acceptable variations of the probe voltage
control values. Above that a blocking error
is returned.
Do not use too high delta values, otherwise you are not likely to detect a
blockage or a homogeneity problem.
It goes without saying that in this case the results sent back by the apparatus are
likely to be erroneous.
As with too small a deltas, there is a likelihood of setting off small blockage or
homogeneity alarms at any untimely moment (especially with low value results).

6.5.4 Serial configuration
This dialogue configures the apparatus's serial ports.
These options enable you to ask for the results to be sent either with or without
graph data.
If the option 'Manual send' is checked, the data's exportation can only be carried
out on the serial port with the export dialogue.
For a full description of the transmitted data format, refer to this manual's
appendix.

6.5.5 Parameters
This dialogue selects the parameters you wish to be displayed.
If the parameter is checked it will be displayed.
6.5.6 View
Reserved for future functionalities.
6.5.7 Identification
This dialogue enables you to choose and configure the required identification
mode.

The apparatus proposes four identification modes.
1) Manual mode
The operator must enter an identification's name for each analysis.
2) Automatic mode
The automatic incrementation mode enables you to key a set part of the
identification's name and the apparatus will increment the value of each analysis.

Example :
If the set part of the patient's identification is analysis0 and the increment 1, the
identification's names will be :
analysis1
analysis2
...
analysis10
etc.
3) Automatic date mode
The automatic date mode is identical to the automatic mode, but the set part
corresponds to the day's date with the following format :
YYYYMMDD-XXX
YYYY being the current year

MM the month
DD the day
XXX being the number incremented for each analysis of the keyed value in the
'Increment' filed.
4) Work list
For the work list's functionalities' description, refer to the 'Identification' chapter in
this manual.

The current work list's status displays :

- The next patient's file number.
- The number of analyses it contains.
- The number of analyses carried out.
When the last programmed analysis in the work list is finished, the
apparatus toggles to the manual identification mode.
6.5.8 Blank
The 'Blank' parameter configures the different parameters used during an empty
analysis.

To do a 'blank' analysis, use the 'Ctrl' + 'B' command, or the 'Analysis' menu
then 'Blank'.
Such an action verifies the apparatus's contamination status.
1) 'RBC' Group
The first parameter, 'Max value for blank' sets the RBC maximum value for which
the empty analysis will be considered as a blank.
In this case :
- If the 'Null forced' option is selected in the display parameters, the results in this
'empty' analysis will be nil.
- If the 'Save blank' option in this dialogue is not checked, the results will not be
registered.
2) 'Background threshold' group
These values represent a threshold beyond which a contamination alarm will be

set off for the channel concerned.
The lower the value, the higher the contamination sensitivity will be.
A badly rinsed machine, the end of a flask or a past expiration period date can
set off this alarm.
In this case, you are advised to check the state of the reagents flasks and carry

out a chambers cleaning with the technical functions.
This type of alarm can only take place on a 'Blank'.
6.5.9 Password
This dialogue chooses the level of the rights set by default to starting up the
apparatus or modifying the passwords.
The apparatus has three user levels.

- Standard 'user' mode
- 'Super user' mode
- 'Administrator' mode
1) Regulating the 'by default' level

You can set the right user level at the start. To do that, select the required level in
the roll-down list.
2) Modifying passwords.
To access super user or administrator level you have to key in a password.
This dialogue modifies the apparatus's passwords.

Choose the level on the left, then key in the new password in the two fields, then
validate.
For full information of the agreed to rights in function to the chosen level,
refer to the 'Services' chapter in this manual.
6.5.10 Export
This dialogue parameters certain delimiter characters during the analyses' results
exportation.

1) Exportation under text file form
To export one or several analyses, refer to the 'analysis' chapter.
- Configuration of the field separation character.

This character appears between each result of the same analysis. It is set by
default with a semi-colon.
- Configuration of the decimal symbol

Decimal point by default.
2) Exportation on a serial port
- Configuration of a decimal symbol

It is the decimal point by default.

6.6 Units
6.6.1 Regional Parameters
This dialogue chooses a standard unit set, as well as the language used for
wording the parameters.
The choice of the norm used determines a set of units replying to a standard.
To modify the standard, you have to select one from the list then validate it by
clicking the 'Modify' button.
We will see afterwards that it is possible to modify the parameters' wording as

well as the units.
The language used by the apparatus's parameters' names is configured in the

program's language by default.
It is possible to modify them with the 'Modify' button'.
It is possible to have, for example, an apparatus with a program in English

and the parameters' labels in French :


6.6.2 Display format
This dialogue modifies the display of each parameter.
The roll-down list enables you to choose the parameter to modify.
The 'Normal' box on the bottom right displays the parameter as it will be
displayed, so you can check the modifications made before validating them.
1) 'Format' box
Parameter word (7 characters max)

Its display format (5 characters max)
0 = A number
# = A number <> 0 or a space
The test value to display in the 'Normal'
box
Various display options.
2) Unit box

To change the unit's word or exponent
Unit word (5 characters max)

Multiplying or dividing coefficient.
Use the up and down arrows to modify

the exponent's value.
Unit word (5 characters max)

Multiplying or dividing coefficient.
Example : WBC display in hundreds/mm3 with an exponent type display.
The coefficient goes from 1 to 10 to compensate for the exponent (from thousand
to hundred per mm3).
The 'Normal' box displays the parameter as it will appear by taking these
modifications into account.

Services

100 MS9-5 Operator manual Services
7 Services
7.1 Banks
7.1.1 Presentation
Many of the apparatus's parameters are programmable :
- Some are the machine's own global functioning and are grouped in the
configuration system and fluidics dialogues.
- Others are the blood analysis's own nature. The whole of the latter are grouped
in a 'Bank'.
Each time you select a bank, you are reconfiguring the apparatus according to
the chosen bank's parameter values.
The apparatus is provided with a certain number of predefined banks. You may
modify these parameters or even create your own banks.
Modifying a bank's parameter is possible but only for a well-informed user.

Any value modification may bring about not only the modification of the obtained
results and the machine's CV, but also and above all the validity itself of this.
In the case when you want to modify certain parameters all the same, we advise
you to work on a duplicate bank (see 'Creation' bank section) rather than the
original.
The subject of this chapter is to show how to modify a bank's parameters and not
a functional description of these parameters. For that, refer to the 'reference
manual'.
7.1.2 Selection
When starting the apparatus, a bank is chosen by default.

This bank's 'by default' choice may be changed (see : 'Parameters' menu,
'System' item, 'System' tab, 'Default bank' field).
The default bank is displayed in the tool bar :
The selected bank for the next analysis is displayed in the bank zone of the next
analysis.
- File number = '20030111-002'

Services MS9-5 Operator manual 101
- Selected bank = 'MALE'
If you wish to change the bank for the next analysis just select the required bank
in the tool bar :
To have :
and for the next analysis :
Any bank change must be done before a new analysis. Once the analysis
has started, it is impossible to modify the bank.
The 'work list' mode is irrespective of the bank displayed in the tool bar.
7.1.3 Modification
We would like to remind you that an existing bank's parameter modification

is possible, but only for a well informed user.
Let us now look at how to access and modify a bank's parameters :
To select the required bank :

'Services' menu, then 'Banks' or the F5 function key.

One choice amongst all the existing banks is proposed :
Select the required bank, then click on the 'Edit' button, a new window is
displayed.
The bank's name is displayed in the top left of the window. In our case it is the
'MALE' bank.
This window consists of several tabs and two buttons.

You may abort these current modifications at any time by pressing on the 'Cancel'
button.
If you press on the 'Ok' button, all the performed modifications will be taken into
account.
A bank's different parameters are grouped by type and accessible by the tabs
located on the top of the window.
Click on the corresponding tab to access a value group.
7.1.3.1 Threshold
Threshold values correspond to a cell discriminator.
This selection is set up by channels in accordance to four criteria :

- Impulse minimum range or low threshold.
- Maximum range or high threshold.
- An impulse's minimum width.
- Maximum width.
Cells that do not correspond to these criteria are rejected.
The threshold tab is shown in the following form :
Threshold values can vary from 1 to 255 with a minimum value inferior to the
maximum value.
To modify a value, use the up and down arrows on the value's right to
respectively increment and decrement the value.
For a full description of a bank's threshold adjustments, refer to the 'Cell

Measurement' chapter in the reference manual.

7.1.3.2 Gain Modification
The gain represents the amplification value of each channel's signal.
The gain is a programmable value from 0 to 255. The 0 value cuts the channel.
An increasing value raises this gain.
To modify a gain, use the up and down arrows on the right of the value.
For the full description of the bank's parameters, refer to the 'Cell Measurement'
chapter in the Reference Manual.


7.1.3.3 Modifying the counter’s time
The counter's time may be defined for each channel.
The counter's time modification is taken into account automatically during the
calculations of the results.
To modify a time, use the up and down arrows on the right of the value to
respectively raise or lower it.
A too low a counter's time may affect the machine's CV or set off
homogeneity alarms.
Conversely, a too high a counter's time may empty the chambers under the
counter orifices' level and cause completely erroneous results.
7.1.3.4 Modifying the sensor voltage
For a full description of probe voltage values, refer to the 'Cell Measurement'
chapter in the reference manual.
The probe voltage is a one tenth volt value applied to the measurement
chambers' electrodes' terminal.
The size of the impulses depends on this voltage.
The apparatus corrects the MCV value in function to the probe voltage applied to
the RBC/THR.

To modify a value, use the horizontal cursors under the values.

A movement to the right will increment the voltage and likewise a movement to
the left will decrement it.

7.1.3.5 Smoothing
Smoothing parameters are grouped into two categories :
- parameters used to calculate the results.

- parameters used during the histograms' display and printing.

For a full description of the smoothing parameters and their respective actions,
refer to the 'Cell Measurement' chapter in the reference manual, 'Histogram
smoothing' paragraph.
The 'Smoothing number' parameter is a repetition counter.

The 'Size' parameter corresponds to the width of the matrix applied to the curve.
1) Smoothing calculation
The parameters used for the calculation compensate the apparatus's non-
linearity for the low values. It is not recommended to modify the factory values.
2) Smoothing display
These parameters only have an aesthetic role at printing display level. However,
it is not recommended to apply to high a smoothing coefficient that could conceal
certain defects in a curve.


7.1.3.6 Min/max volumes
This tab groups three zones together :
1) 3DIFF zone
This zone groups together 8 values intended for modifying the cursors' position
for a formula approach : Lympho, Mono, Granulo.
NB. These values are only used in 3 DIFF mode. For a full description refer to the
apparatus's reference manual.
The 'Start' and 'End' values define their own zone for each type of cell for which
the formula will be calculated.
Lympho start, lympho end

Mono start, mono end
Granulo start, granulo end
2) 3DIFF/5DIFF zone

These cursors are used in the two modes and only concern the RBC and
platelets channel.
3) 5 DIFF zone
Only in 5 DIFF mode and only concerns the white blood cells. For a 5DIFF
min/max description, refer to the reference manual.
LMG formula values found may be different in 5DIFF mode and in 3DIFF
mode. The latter being only a formula approach.
7.1.3.7 Quantities
The quantities parameters regulate the quantity of sample blood as well as the
lyse quantity for each dilution.
1) 1st test
The first parameter group concerns the blood, eosinos lyse and if necessary
diluent quantities used for eosinos chamber dilution.

- Quantity of blood
taken for dilution in
the eosinos chamber.
- Eof-diff. lyse
quantity.
- Quantity of
additional diluent for
the eosinos dilution.
- Not used.
The 'Additional time' parameter is not accessible for the human MS9-5 in the
present version.
2) 2nd test
- Quantity of blood for

pre-dilution.
- Quantity of lyse for

the WBC chamber
dilution.
7.1.3.8 Calibration
For an everyday use, there is not need to modify the calibration bank's dialogue.
But the machine's general calibration may not be sufficient with certain types of
blood and for certain parameters. So you may have to adjust the results by
modifying their calibration coefficients at the bank's level.
These coefficients will be applied, during the calculation of the results, by

complementing those of the machine.

A coefficient to one produces no effect.

7.1.3.9 Correction
The bank correction panel corrects a results' linearity gap for raised values.
The apparatus's standard adjustments have been calculated in such a way to
provide a largest possible linearity range.
But it is possible to lose this linearity for particular adjustments for high values
(i.e. very high blood cells quantity).
Correction coefficients restore this linearity for high values.

A coefficient equal to 0 cancels all correction.
7.1.4 Creation
'Services' menu then 'Banks' or the F5 function key :
To create a new bank click on the 'New' button.

Give it a name and choose a reference bank as a model :
Validate by pressing 'Ok' button.
The TEST bank thus created will be the carbon copy of the MALE bank, that is to
say :
1) All the new bank's parameters will be identical to those of the model bank.
2) The 3 DIFF and 5 DIFF cursors' definitions will be identical.
3) The pathological alarms (if the model bank has them) will be identical.
To create its own bank, choose a model bank that is the closest to it.
The new bank thus created will be available for the next analysis.
7.1.5 Deletion
Several conditions are necessary to delete a bank.
1) You can only delete a bank that you have created beforehand. Banks provided
with the apparatus are locked and therefore the 'Delete' button is not valid.
2) For the moment, it is not possible to delete a bank that is not locked if the
analyses have not been carried out with it.

A future option (program version > 1.2 build 9) will enable you to lock and
unlock a bank as well as deleting it with all the analyses concerning it. This
option will only be possible with the machine's 'administrator' rights (Refer to the
chapter concerning the use of passwords).
7.2 Pathologic flags

7.2.1 Presentation
The pathologic flag concept corresponds to a mechanism that creates a bank's

own flags according to certain criteria.
Characteristics :
- An flag is linked to a bank.
- An flag is linked to a given parameter.
- Up to 5 logical operations are defined making the conditions play part on the
different parameters.
You may define for each flag :

- A name.
- A particular character or symbol in the fonts of your choice that will be displayed
next to the parameter if the alarm is set off.
- A particular color or size for this character or symbol.
- A 1 to 5 priority level (5 being the highest level).
- A type of alarm :
a) Minimum : this type defines a low normality for a parameter.
b) Maximum : this type defines a high normality for a parameter.
c) Other : all the other types of alarms.
- A description linked to the alarm. This description will be printed automatically
at the bottom of the results if its type is 'other'. The minimum and maximum type
normalities are printed on the parameter line.
- One or several setting off conditions (up to 5).

If several flags are set off simultaneously for one same parameter, the flag
with the strongest priority will be displayed.
7.2.2 Creation
Creating a pathologic flag.
'Services' menu then 'Pathologic flags'.
1) Choice of associated bank :
2) Then 'New' button

The dialogue consists of two parameter groups, the first group defines the flag,
and the second configures the setting off conditions.

3) For the 'MALE' bank to define, for example :

- a name : Thalassemia.
- an associated parameter : RBC (parameter selection in the roll-down list).
- a symbol : letter T ('Change' button, then fonts and character choice).
- a color : yellow ('Change' button and desired color choice).
- a type : 'Other' (in the 'Type' box).
- a description : Let's enter, for example, 'Further investigate for Thalassemia' in
the 'Description' field.
- a priority : 2 (choice of level with up and down arrows on the right of the 'Priority'
field).
- the conditions :
We are going to choose the criteria to set off the flags, for example : RBC > 5.9
millions and MCV < 83 fl.
To create these two conditions :
- In the 'Conditions' box click the 'New' button to create the first condition : RBC >
5.9.

For the first logical operation, the condition has to be AND, the parameter is RBC,
the comparison operator is the sign superior to the 5.9 M/mm3.
Begin the same operation again to create the second condition :
The logical operation is the 'AND' operator, the associated parameter MCV, the
comparison operator is the sign inferior '<' and the value of MCV 83.0 fl.
Click the 'Save' button to register it.
Now that the two logical conditions have been created, the criterion for setting off
the alarm has to be defined.
Linking the two logical operations with the 'AND' operator
The two conditions must be satisfied to set off the alarm.
Select the 'AND RBC > 5.9' in the roll-down list on the left of the 'Conditions' box,
then click the arrow button to the right '-->'.
The condition is displayed in the right column.
Do the same for the 'AND MCV < 83' condition.
The two conditions are displayed in the list on the right.

NB : to remove a condition from the list on the right, click on the arrow to the left
'<--'
The alarm is now created. To save it, click on the 'Save' button.
To sum up :
During an analysis carried out with the 'MALE' bank, if the setting off criteria are
satisfied, a 'T' symbol (in yellow) will be displayed next to the GR parameter.
The contents of the 'Description' field will be printed at the bottom of the analysis
in the 'pathologic flag' zone.
Remarks on the alarms and logical conditions :
1) The pathologic flags and logical conditions are linked to a data bank.
2) If a logical condition for a data bank is modified, the setting off value of all the
pathologic flags that will use this condition will be modified.
3) When creating a new bank, the pathologic flags as well as the logical condition
defined in the model bank will be duplicated.
Specific case of 'Minimum' and 'Maximum' types :

This type of alarm is also used to define the normalities for the associated bank.
The setting off conditions are identical.
The main difference with a 'Other' type alarm is the printing of the results.
1) The normalities that are printed at each parameter level come from alarms of
this kind.
2) The 'Description' field is not printed in the 'pathologic flag' zone for these
alarms.
To modify a normality, you have to modify the condition associated to the

alarm (see above : modification of the RBC's maximum value for the
'Thalassemia' alarm).
7.2.3 Modification
The modification process of a pathologic flag resembles that of a creation one.
Take the 'Thalassemia' flag.

We now wish the 'T' symbol to be displayed at the MCV parameter level and not
as before for the RBC parameter.
'Services' menu the 'pathologic flag'.
1) Choose the 'MALE' associated bank and select the 'Thalassemia' alarm then
'Modify' button :

Change the 'RBC' parameter value for 'MCV' in the 'Parameter' roll-down list :
Then save the modification.
Specific case of a logical condition :

If you wish to change the criterion or criteria of a logical condition proceed in the
following way :
Select the condition in the roll-down list on the left :

For example RBC > 5.90 then 'Modify' button.
Now change the 5.90 value to 5.70 and save.
The modification carried out on the logical condition will automatically be reported
to the column on the right :

Save the alarm so modified.
7.2.4 Deletion
The pathologic flag deletion process is as follows.
'Services' menu then 'Pathologic flags'
Select what you want to delete in the flag list. Then 'Delete' button.

7.3 Technical functions
The technical functions panel is presented in the form of a roll-down list where
the different functions are classified in alphabetical order.
The technical functions mainly correspond to the machine's maintenance

operations and may be catalogued in these different groups :
- Priming functions mainly used during reagents pack replacement.

- Cleaning functions used after the machine has been stopped for a long time or
during a high contamination.
- Unblocking functions for each chamber.
- Emptying functions.
- Machine testing functions.
To start up a fluidics function, select it in the roll-down list then click 'Execute', if
not 'Quit'.

7.4 Stand-by
The stand-by mode can either be done automatically at the programmed time in
the dialogue system, or carried out manually : 'Services, Activ. Stand-by' menu
or a combination of the 'Ctrl' + 'V' keys.
A stand-by mode panel is displayed during the length of the operation :
At the end of the operation check the apparatus's status in the status bar at the
bottom of the screen :
An apparatus in stand-by mode can only be operational after having carried

out a full priming.
7.5 Password
Access to certain of the apparatus's functions depends on the agreed levels of

rights.
There are three user levels :
1) 'Standard user' level or level 1
The user may :

- Carry out an analysis.
- Key the patient's identification information.
- Have access to technical functions.
- Put the machine in sleep mode manually.

2) 'User with authority' level or level 2
The user has all the previous rights plus :

- Carrying out an analysis for quality control.
- Modifying the display and printing parameters.
- Have access to parameter systems.
3) 'Administrative' level or level 3
This user level has the highest rights.
To access each of these levels, you need to enter a password. To check which
level the apparatus has at present, access the 'About' dialogue and note the
number you find under the padlock.
In the above example, the apparatus is at level 3 or 'Administrative' mode.
To change this present level, select 'Password' in the 'Services' menu, then enter
the password corresponding to the required level.
Validate with the 'Ok' button.
To modify the passwords, click the 'Change' button.

Select the level on the left and key the new password.
The 'Change' button, giving access to modifying passwords, is only

accessible to the 'Administrative' mode.
You can determine the agreed level by default when starting up the apparatus.
To do this, refer to the 'Parameter systems' chapter in this manual.

Appendix

128 MS9-5 Operator manual Appendix
8 Appendix
8.1 Format connection series
Conditions for passing on data to a computer :
1) Connect the apparatus to your computer with a 'crossed' cable.

2) Configure the apparatus's serial port. (Menu : 'Parameter system' then 'Serial
port')
The decimal separation character may be modified :

For example, you can replace the comma with a dot (configuration by default).
The apparatus can transmit the analyses' results at different times :
1) Either at the end of the analysis : automatic mode (to do that you have to
invalidate the 'Send manually) option in the parameter system.
2) Or several analyses at the same time : manual mode (result of a request
carried out on the stocked analyses).
For more information, refer to the 'analysis – Exportation' chapter.
8.1.1 General structure
unterEach analysis is made up of several groups, each group may involve

several fields.
Each field finishes with a CR (13 decimal code), including the first (STX) and the
last (ETX, EOT).
The first group of an analysis always has the STX character (code 02) and the
last an ETX (code 03).
An EOT character (code 04) is sent at the end of the transmission (irrespective of
the number of transmitted analyses).
Description :
1) Beginning group
Field number Contents
1 STX CR
b) Identification group

Appendix MS9-5 Operator manual 129
1 Diff (3 pour 3DIFF, 5 pour 5DIFF) CR

2 Date CR
3 Time CR
4 Number of one sole analysis CR
5 File CR
6 Name CR
7 First name CR
8 Operator CR
9 Department CR
10 Division CR
11 Comments CR
12 Bank CR
2) Result group
1 'HEMATO' CR
2 Counter CR
3 --> 3+counter Results CR
3) Alarms group
1 Alarms CR
4) Histograms
1 'THR' CR
2 Quantity of platelets for volume 1 nnN+CR
../.. nnN+CR
257 Quantity of platelets for volume 256 nnN+CR
1 'RBC' CR
2 Quantity of red for volume 1 nnN+CR
../.. nnN+CR
257 Quantity of red for volume 256 nnN+CR

(only in 3DIFF mode)

1 'WBC' CR
2 Quantity of white for volume 1 nnN+CR
../.. nnN+CR
257 Quantity of white for volume 256 nnN+CR
(only in 5DIFF mode)

1 'EOS CR
2 Quantity of eosinos for volume 1 nnN+CR
../.. nnN+CR
257 Quantity of eosinos for volume 256 nnN+CR
5) End of analysis
1 ETX CR
6) End of transfer
1 EOT CR
8.1.2 Detailed format
1) Identification
Character 'n' is an optional number which can be replaced by a space, 'N' is

a number always sent and '_' is a space.

Field name Length Format

1 Diff 1 char. + CR N
2 Date 10 char. + CR dd/mm/yyyy
3 Time 5 char. + CR hh :mm
4 Number 8 char + CR nnnnnnnN
5 File 25 char + CR
6 Name 25 char + CR
7 First name 25 char + CR
8 Operator 25 char + CR
9 Department 25 char + CR
10 Division 25 char + CR
11 Comments 255 char + CR
12 Bank 20 char + CR
2) Results
1 Synchro tag = 'HEMATO' 6 char + CR

2 Parameter counter 2 char + CR

3 WBC 7 char + CR nnnN.NN

4 Lym% 7 char + CR nnnN.N_
5 Mon% 7 char + CR nnnN.N_
6 N/Gr% 7 char + CR nnnN.N_
7 Eos% 7 char + CR nnnN.N_
8 Bas% 7 char + CR nnnN.N_
9 Others% 7 char + CR nnnN.N_
10 Lym# 7 char + CR nnnN.N_
11 Mon# 7 char + CR nnnN.N_
12 N/Gr# 7 char + CR nnnN.N_
13 Eos# 7 char + CR nnnN.N_
14 Bas# 7 char + CR nnnN.N_
15 Others# 7 char + CR nnnN.N_
16 RBC 7 char + CR nnnN.NN
17 MCV 7 char + CR nnnN.N_
18 Hct 7 char + CR nnnN.N_
19 MCH 7 char + CR nnnN.N_
20 MCHC 7 char + CR nnnN.N_
21 Hb 7 char + CR nnnN.N_
22 RDW-SD 7 char + CR nnnN.N_
23 RDW-CV 7 char + CR nnnN.N_
24 µRBC 7 char + CR nnnN.N_
25 MRBC 7 char + CR nnnN.N_
26 THR 7 char + CR nnnN___
27 MPV 7 char + CR nnnN.N_
28 Pct 7 char + CR nnnN.N_
29 Mod 7 char + CR nnnN.N_
30 Medn 7 char + CR nnnN.N_
31 PDW 7 char + CR nnnN.N_
32 µTHR 7 char + CR nnnN.N_

3) Alarms
1 Alarms 10 char + CR nnnnnnnnnN
List of alarms :
ALARME_AUCUNE = 0
Clot alarm :
ALARME_COMMANDE_SONDE_WBC = &H1
ALARME_COMMANDE_SONDE_RBC = &H2
ALARME_COMMANDE_SONDE_EOS = &H4
ALARME_TENSION_SONDE_INITIALE_WBC = &H8
ALARME_TENSION_SONDE_INITIALE_RBC = &H10
ALARME_TENSION_SONDE_INITIALE_EOS = &H20
ALARME_TENSION_SONDE_COMPTAGE_WBC = &H40
ALARME_TENSION_SONDE_COMPTAGE_RBC = &H80
ALARME_TENSION_SONDE_COMPTAGE_EOS = &H100
Haemoglobin measurement problem

ALARME_HGB = &H200
Air leak
ALARME_DEPRESSION_INITIALE = &H400
ALARME_DEPRESSION_COMPTAGE = &H800
Homogeneity
ALARME_HOMOGENEITE_WBC = &H1000
ALARME_HOMOGENEITE_RBC = &H2000
ALARME_HOMOGENEITE_PLT = &H4000
ALARME_HOMOGENEITE_EOS = &H8000
Reserved
ALARME_RESERVEE = &H10000
ALARME_RESERVEE= &H20000
Contamination
ALARME_CONTAMINATION_WBC = &H200000
ALARME_CONTAMINATION_RBC = &H400000
ALARME_CONTAMINATION_PLT = &H800000
ALARME_CONTAMINATION_EOS = &H1000000
An alarm is set off if the result of one AND logic between the received value and
one of the above masks is not nil.

8.1.3 Configuration
1) Serial port configuration :
To configure the serial port :

'Parameters' menu then 'System' then the 'Serial configuration' tab
or
F12 Function key then the 'Serial configuration' tab.
The 'Serial configuration' tab consists of 3 boxes :
a) Configuration
To define the speed, parity, data bit number, stop bit number and protocol..
b) Port
To select the serial port used.
c) Group options
In this version you can :
- Send histograms or not.

- Send it manually.

If the 'Send histogram' option is checked, the histogram data is sent with each
analysis.
In 3DIFF mode : histogram for platelets, RBC and WBC.

In 5DIFF mode : histogram for platelets, RBC and eosinos.
2) Export
You can define the decimal symbol that will be sent.
'Parameters' menu then 'System' then 'Export' tab

or
F12 function key then 'Export' tab.
'Serial port' box
The symbol used by default is the one defined in the Windows configuration
panel.
In US/UK version = '.' (dot)
In French version = ',' (comma)
You may define the symbol of your choice, for example a dot instead of a comma.

8.1.4 Exporting the data
There are several possibilities of sending the results of the analyses.
1) Manual mode :
Check 'Send manually' in the 'Serial port' configuration panel.

In this case, the only means of sending the analysis's results is to use the
selection mode.
2) Automatic mode :
Uncheck 'Send manually' in the 'Serial port' configuration panel and the results
will be sent automatically after each analysis on the serial port you have
configured.
3) Selection mode :
The exported analyses are the result of a data base request.
To access the requested page :
a) 'Analysis transfer' button in the toolbar or

b) 'Analysis' menu then 'Export'.
The proposed date is that of the day (checked date) and the comparison operator

by default is '='.
If you wish to display the day's analyses, you only have to carry out your search
('Search' button).
The analyses will be displayed, the 'Total' label will indicate the number of
analyses found.
To export the data : 'Export' button.
You will be given a choice : 'File' export or 'Serial port'.

Select 'Serial port' and validate.
The 'Total' label will indicate the transfer's progress : n/nnn.
The transfer time depends on the chosen speed and the number of analyses to
be exported.
You may interrupt the transfer at any time : 'Quit' button.
Parametering a request :
a) Date zone :
If the date is ticked, the request will be carried out for the requested date with the
chosen operator date :
= : to the defined date
>= : from the defined date
<= : before the defined date
b) 'Max' counter
This counter limits the number of extracted analyses during a request.
Ex :
To find the last 200 carried out analyses :
- Uncheck the date
- Initialise the Max counter to 200
- Carry out the search
c) The 'Name', 'File', Operator', 'Department', 'Division' and 'Bank' zones refine
your search.
To search a given patient's analysis or analyses with his name :
Key the name in the 'Name' zone and make a request.
Remark :
You can you the symbol '%' as a substitution character :
- '%SON' will find all the patients whose name finishes with 'SON'.

- In the same way, 'JEF%' will find all the patients whose name beginnings with
'JEF'.
You use the same operation to find a file number.
The can combine the criteria :
Example :
You can recall all the analyses carried out by one given operator, a precise bank
and from a given date.
8.1.5 Connection Cable
Connection cables MS9-5 <-> PC
1) PC 9-pin connector
MS9-5 (9 pin) PC (9 pin)
2 3
3 2
4 6
5 5
6 4
7 8
8 7
1) PC 25-pin connector

MS9-5 (9 pin) PC (25 pin)

2 2
3 3
4 6
5 7
6 20
7 5
8 4
8.2 Long lasting stop
The procedure is the following :
1) Remove the reagents tubes and put them into a container with distilled water
except, of course, for the waste tubes.
2) Go to the 'Technical services' :

'Service' menu then 'Technical functions' and start up the following technical
function 'General rinsing with distilled water'.
Repeat this operation 3 or 4 times.

At the end you may leave the tubes in the distilled water.
When starting up the apparatus again, it is important to carry out several

long primings with the reagents.

8.3 Notes

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MS9-5 H AUTOMATIC

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Part III Identification 40

Part V Quality control 60

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Part VII Services 100

Part VIII Appendix 128

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Choose a suitable place to set up the machine.

Electric connection should be with an earth point. A good earth connection is

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1.2.2 Initialisation of the system

Starting the system is carried out in several steps :

- Loading the Windows running system.

The version's language is found in the 'Language' zone.

The apparatus is configured in the destined country's language during its

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In case of a problem, if the auto-test procedure detects a mechanical or

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a) Priming the Hemoref

b) Cleaning the needle with Hemoref

c) Priming the reagents pack

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The word 'Ready' appears in the status line at the bottom.

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1.2.3 Main display

This zone is at the top of MS9-5's main window.

The apparatus handles keyboard shortcuts.

2) Toolbar and bank selection

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few seconds the function's description is displayed.

An identification zone consists of three elements :

There are three identification zones :

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The result zone is made up of three groups :

- Zone 1 : white blood cells group.

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5 DIFF mode Graphs 3 DIFF mode Graphs

6) Monitoring count zones

Beginning on the left :

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2) Blood sample tubes

It is recommended to not take a blood sample from a patient under anaesthetic.

Before carrying out an analysis on the apparatus, it is important to perfectly

It is essential to carry out a strong and long homogenisation if :

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- The blood is sedimented.

- The selected bank : male, female, new born, other...

Click with the mouse on the arrow of the roll-down list :

Select the required bank :

2) Possible change of mode

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Shake the tube well up and down several times.

It is essential to carry out a strong and long homogenisation if :

4) Check the tube's adapter

Each kind of tube has a specific adapter.

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2.1.3 Analysis identification

You can key in the analysis's identification at different times :

- When preparing a tube series :