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FOREWORD

Analysis of Urine and Body Fluids laboratory manual contains activities which have been
sensibly designed and logically formulated to facilitate learning from the various discussions in
the lecture. The activities have been carefully selected, simplified and ingenuously made to
emulate the procedures and processes in the clinical laboratory prior to clinical internship.
However, it is to be noted that most of the procedures stated will depend on the available
materials, equipment and reagents provided for by the institution’s laboratory.

To enhance the retention of important facts about Clinical Microscopy, the pre-laboratory
discussions and the learning contents in every activity have been enhanced by sourcing
information from different reference books available. Therefore, students are encouraged to read,
understand and learn from this academic output.

It is aimed that one laboratory meeting will suffice for the Physical and the Chemical
examination of urine and a more extensive training for the Microscopic examination enabled since
the latter is the core of Urine Analysis.

Learning activities on Analysis of other Body Fluids are patterned according to standard
procedures applied on the actual practice of laboratory science to provide students a clear
overview and comprehensive explanation of the different methodologies. Thus, it is expected that
students will ultimately develop technical skills after performing the different experiments. For
student’s self- rationalization, post laboratory sheets with guide questions are given at the end of
every activity.

Koudai I. Albaira, RMT


Noraine Princess G. Tabangcora, RMT
January 2017
TABLE OF CONTENTS

TITLE PAGE
FOREWORD

LEARNING ACTIVITY TITLE PAGE


NUMBER
1 The Proper Collection and Observation of an Ideal 1
Urine Specimen for Urine Analysis with Physical
Examination

2 The Proper Technique of Performing Chemical 15


Examination of Urine Using Reagent Strip and
Conventional Methods

3 The Proper Way To Identify And Report Different 36


Urinary Sediments

4 The Proper Way To Perform And Report One-Step 51


Qualitative Urine Pregnancy Test

5 The Proper Way To Perform Fecal Occult Blood Test 59

6 The Proper Way To Perform the Collection, Storage, 66


Transport and Analysis of Seminal Fluid

REFERENCES

BENCH AIDS ON IDENTIFICATION OF URINARY


SEDIMENTS
REFERENCES

Brunzel, N. (2004). Fundamentals of Urine and Body Fluid Analysis. 3rd edition.

McPherson, R., & Pincus, M. (2011). Henry’s Clinical Diagnosis and Management by
Laboratory Methods. 22nd edition.

Mundt, L., & Shanahan,K. (2011). Textbook of Urinalysis and Body Fluids. 2nd edition.
Strasinger, S., & Di Lorenzo, M. (2014). Urinalysis and Body Fluids. 6th edition

World Health Organization. (2010). WHO laboratory manual for the Examination and
processing of human semen. 5th edition.

American Association of Clinical Chemistry.


http://www.aacc.org/publications/cln/2011/march/Pages/FecalOccult.aspx

Hemacytometer and Diluting Fluid Practice.


http://biology.clc.uc.edu/fankhauser/Labs/Anatomy_&_Physiology/A&P202/Blood/Blood_Counts
_practice.htm

Human Chorionic Gonadotropin Test


http://www.dovemed.com/human-chorionic-gonadotropin-test/

National Institute of Diabetes and Digestive and Kidney Diseases.


http://www.catalog.niddk.nih.gov/imagelibrary/searchresults.cfm?keyword=1505&type=keyword

Pregnancy hCG Cassette


http://www.medicaldisposables.us/hCG-cassette-pregnancy-test-p/w1-c.htm

Test For Success: The Semen Analysis


http://attainfertility.com/article/semen-analysis
Department of Medical Laboratory Science

ANALYSIS OF URINE AND OTHER BODY


FLUIDS

Laboratory Manual

Compiled by
Koudai I. Albaira, RMT
Noraine Princess G. Tabangcora, RMT