CFB 40503 PRACTICAL

MALAYSIAN INSTITUTE OF CHEMICAL AND BIOENGINEERING

TECHNOLOGY.

INSTRUMENTAL FOOD ANALYSIS (CFB 40503)

UNIKL MICET
DOC. NO. : JAN 18
LAB MANUAL

PRACTICAL 1: COLOUR ANALYSIS

OBJECTIVE

1. To determine colour properties of food products using Lovibond tintometer and

Chromameter.

INTRODUCTION

Colour is one of the three principal quality attributes that influence the food acceptance and
judgement. Customer examines the food product and expecting the colour must be ‘right’.
Instrumental colour measurements are extensively applied due to subjective assessment through
human’s visual. The colour measurement instrumentals provide stability, easy to use and most
importantly achieve the desired objective colour measurement for both research and industrial
applications.

List of Manuals needed

1. Lovibond tintometer
2. Chromameter

Samples
Two types of samples needed
1. Liquid samples – drinks with some coloring
2. Solid samples – fruits, labels etc

Methodology

Sample preparation
1. Liquid samples – degas the carbonated drinks. Separate drinks into three parts – original
conditions and dilute it into 50% and 25% of original conditions. You will need a
minimum of 25 ml from each parts.
2. Solid samples – to use as it is.

Lovibond tintometer
1. This system need to be calibrated.
2. Ensure that you have selected the Transmission or X, Y, Z mode for the determination.
You can do using the Mode selection button.

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 CFB 40503 PRACTICAL

3. Prepare samples into cuvet, you will need about 15 ml liquid. Press READ button and
Press PRINT button after sometimes.
4. Remove existing sample for the next determination.
5. Rinse cuvette with next sample and fill it with sample to be determined. Repeat step
number 2.

Chromameter
1. Calibrate the chromameter
2. Prepare sample into cuvet of the system. You will need about 10 ml liquid sample.
3. Press button on the system to determine the reflextance value. You may need to
reposition the sample for another 2 times during data acqusitions.
4. Remove existing sample for the next determination.
5. Rinse cuvet with next sample and fill it with sample to be determined. Repeat step
number 2.

Data collection
1. Please ensure that you have read and understand the operation manuals for both
Lovibond and Chromameter. Brief operation for both system is included in this Manual.
2. For Lovibond tintometer, adjust the system to get data for X, Y and Z and data for
Transmission from 410 to 700 nm. This data will be used in your report.
3. For Chromameter, adjust the system to get a list of data for the X, Y, and Z; L ab and L
a*b*.
4. Use data from tintometer to calculate the x and y value utilizing excel sheet as provided
in the e-learning system.

Result and Discussions

1. Prepare data obtained from the experiments to calculate the X, Y and Z; x and y and to
identify the point for each liquid portion in the x, y chromaticity diagram.
2. Differentiate result between both tintometer and chromaticiy system and discuss the
method that was used by both systems that give results as obtained in your experiment.

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