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Nitrogen is an essential ingredient in the formation of proteins for cell growth. From complex
organisms like animals to the simple bacteria used to treat wastes in an activated sludge
treatment facility, every living thing needs some form of nitrogen to survive.
But too much nitrogen freely available in the environment can be a bad thing. Excess
nitrogen discharged into our waterways can contribute to eutrophication, the gradual change
of water bodies into marshes, meadows, then forests. It can also contribute to massive algae
blooms leading to oxygen depletion in water and its associated problems. Certain forms of
nitrogen can cause specific problems too.
Ammonia is toxic to fish, and nitrates at high enough dosages in the drinking water cause
methemoglobinemia in infants (Nitrates convert to nitrites in the stomach. These nitrites then
interfere with the oxygen-carrying capacity of the haemoglobin in blood).

To test the level of ammonia nitrogen content in the G3 lake.

2.3.4 Ammonia Nitrogen (Nessler Method, Method 8038)

Procedure :
1. Prepare the sample by filling 25 ml of sample cell into the measuring cylinder and
repeat the step for preparing the blank sample by filling with deionized water.

Figure 27: Samples water from G3 lake

2. Add three drops of mineral stabilizer to each sample.
Figure 28: Adding mineral stabilizer
3. Put the stopper on the measuring cylinder. Shake five times to dissolve the reagent.

Figure 29: Shaking the cylinder

4. Add three drops of alcohol to each sample.

Figure 30: Adding alcohol

5. Put the stopper on the measuring cylinder. Shake five times to dissolve the reagent.
Figure 31: Shaking the cylinder to dissolve the reagent
6. Add three drops of Nessler reagent to each sample.

Figure 32: Adding Nessler reagent

7. Put the stopper on the measuring cylinder. Shake five times to dissolve the reagent.

Figure 33: Shaking cylinder to dissolve reagent

8. Transfer 10 ml of the sample into each sample cell bottle.
Figure 34: Transferred sample into sample cell bottle
9. Clean the blank sample cell.
10. Insert the blank sample into cell holder.
11. Push ZERO. The display shows 0.00 mg/L NH3-N.
12. Clean the prepared sample cell.
13. Insert the prepared sample into cell holder.
14. Push READ. Results show in mg/L NH3-N.

Figure 35: Results display

4.4 Ammonia Nitorgen (Discussion)

Ammonia exist in aquatic environments and can cause direct toxic effects on aquatic life.
Basically, it comes from fertilizers and other industrial applications. In this experiment, the
content of ammonia obtained in water sample of G3 lake which collects the runoff from the
road surface and faculties. Based on the experiment, the ammonia content in the water sample
is 0.83 mg/L. This value shows that the water sample have low ammonia content and it can be
classified in Class III based on DOE Water Quality Index Classification. According to this
index, Class III water quality can be used for water supply, fishery and suitable for sensitive
aquatic species to live in. Low content of ammonia will less effect the aquatic life the pond.
Based on our observation, the lake looks clean. During the sampling process, the pH value for
the water sample is 6.4 and it DO is 7.51 mg/L. These two value influenced the result taken for
the water sample. This water sample have low ammonia content and high dissolved oxygen
that encourage the living of aquatic life in the lake.

During the experiment, after all the reagents needed were mixed together with water
sample, the mixture shows clear yellow colour which indicates the low content of ammonia.
The surrounding building produce wastes such as food waste and human waste. These factors
can lead to high rate of water pollution in water. There might also occurred some errors while
conducting the experiment like parallax error and inaccurate measurement of reagents and