The most common types of volumetric glassware are volumetric pipets and volumetric flasks.
These containers are calibrated at a specific temperature to deliver or contain VERY PRECISE
amounts of liquid. Heating the glassware or using heated solutions distorts the calibrated
volume! Volumetric ("vol") containers are expensive and should be handled with care.
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Reading: Volumetric Flasks and Pipets Revised 3/7/12
viewing, a buret card (a white card marked with a dark line) can be held behind the glassware to
contrast the line between glassware markings and the liquid level.
Volumetric Flasks
A volumetric flask is a flat-bottomed flask with a narrow neck (Figure 1). The top of the neck is
specially ground-glass which accommodates a tight-fitting glass stopper or plastic cap. The
calibration mark is a single gradation line on the neck indicates the exact volume the flask will
contain at a specified temperature marked on the flask (usually 20°C). Prevent warming flask
contents (and volume distortions) by handling the flask by the neck instead of the body.
Stopper
Volumetric flasks are used to make solutions of known concentration by the dissolution of a
known mass of solid or the dilution of a more concentrated solution. Before use, always wash
the flask and then prerinse with the solvent. Some frequently used volumes in General
Chemistry lab are 10.00, 25.00, 50.00, 100.00, and 250.0-mL flasks. At times the zeros to the
right of the decimal point are omitted. However, these zeros must always be considered in
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Reading: Volumetric Flasks and Pipets Revised 3/7/12
calculations, as they indicate the accuracy of the volume measurement (i.e., they are significant
figures.)
Volumetric Pipet
A volumetric pipet is an elongated glass bulb with two
narrow glass stems at the top and bottom of the bulb
(Figure 2). The pipet is used "to deliver" a single,
fixed volume of liquid at a specific temperature
(usually 20.0°C) from one container to another. Some
frequently used volumes in General Chemistry lab are
1.00, 5.00, 10.00, and 25.00-mL pipets. Like the vol
flasks, the zeros to the right of the decimal point are
sometimes omitted but are significant figures. The Calibration
Line
bottom tip is tapered to deliver a fine stream of liquid
and is easily clogged. A single calibration mark on the
top stem marks the volume contained at a specific
temperature. Above the calibration mark, stem is open
so a suction bulb can be attached to draw liquid into
the pipet. Note: Never pipet by mouth! Always use a
pipet bulb to provide the necessary suction.
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Reading: Volumetric Flasks and Pipets Revised 3/7/12
paper fibers can draw liquid out of the pipet tip by capillary action.) To transfer the liquid to
another container, simply release finger pressure on the pipet stem and allow the liquid to drain
freely. When finished, touch off the drop of liquid hanging at the tip into the transferred liquid
(it is part of the delivered volume) but do not blow out any liquid remaining inside the tip of the
pipet! The pipet has been calibrated to contain this last drop of liquid.
Caution: Never use a pipet to transfer heated solutions or incompletely dissolved solids. The
heat will distort the calibrated volume and solids can "condense out" on the colder glass walls of
the pipet, clogging the narrow stem and tip.
Mohr pipets have graduated volume markings increasing in value going down the pipet, allowing
for variable volumes to be delivered.
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Reading: Volumetric Flasks and Pipets Revised 3/7/12
(2) Clear tip: Volume markings end with a last marking for the total volume of the pipet (so the
liquid must be stopped before it goes below this last marking and the final volume must be
recorded from the pipet). Like the first type of pipet, once a suction bulb has been used to fill the
pipet, the level of liquid needs to be lowered so its meniscus falls within the markings and then
the initial volume of liquid is recorded. However, for this type of Mohr pipet, dispensing the
liquid requires more coordination because the flow from the pipet has to be stopped once the
amount needed is dispensed and before the volume level falls below the last volume marking.
You should fill the pipet with water and practice dispensing small volumes before using the
chemicals required in an experiment.
Review Questions:
• At what level should your eye be located when reading a volume correctly?
• When using aqueous solutions, do we read the bottom or top of the meniscus?
• Why should volumetric glassware never be washed in hot water or dried in hot ovens?
• Which is more precise: a 50-mL vol flask or 50-mL Erlenmeyer flask?
• Would you use a 200-mL beaker or a 200-mL vol flask if you wish to dissolve about 1 gram
NaCl in approximately 100 mL of water?
• Would you use a 5-mL vol pipet, 5 mL Mohr pipet, or a 5-mL graduated cylinder if you wish
to measure exactly 5.00-mL of water?
• How many decimal places should be used to record the volume of a 10-mL vol flask?
• Where can we find the calibration temperature of a vol flask ?
• Where is the volume calibration mark on a vol flask?
• What kind of pipet can contain variable volumes
• What kind of pipet contains a single, fixed volume?
• Why should a pipet always be rinsed first with the liquid to be used?
• After the liquid is drained from the pipet a small amount remains inside the tip. Should it be
added to the volume drained from the pipet?
• Is it ever safe to pipet a chemical solution by mouth? How about DI water?
• How many decimal places are used when recording the volume delivered by a 10-mL pipet?
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Reading: Volumetric Flasks and Pipets Revised 3/7/12