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# 46

LAMPIRAN 1

## Pembuatan konsentrasi ekstrak daun sirih hijau menggunakan rumus sebagai

berikut:
V1 x C1 = V2 x C2

Ket:

## C2 = Konsentrasi larutan yang akan dibuat (mg/ml)

Perhitungan konsentrasi :

##  20% = 200mg/ml atau 2 gr ekstrak dalam 10 ml etanol 96%

 10% = 100mg/ml atau 1 gr ekstrak dalam 10 ml etanol 96%

 90 mg/ml (9%) : V1 x C1 = V2 x C2
: 10 ml x 90 mg/ml = V2 x 100 mg/ml
V2 = 900 mg/ 100 mg/ml
V2 = 9 ml

 80 mg/ml (8%) : V1 x C1 = V2 x C2
: 10 ml x 80 mg/ml = V2 x 90 mg/ml
V2 = 800 mg/ 90 mg/ml
V2 = 8,88 ml

 70 mg/ml (7%) : V1 x C1 = V2 x C2
: 10 ml x 70 mg/ml = V2 x 80 mg/ml
V2 = 700 mg/ 80 mg/ml
V2 = 8,75 ml

## Universitas Sumatera Utara

47

 60 mg/ml (6%) : V1 x C1 = V2 x C2
: 10 ml x 60 mg/ml = V2 x 70 mg/ml
V2 = 600 mg/ 70 mg/ml
V2 = 8,57 ml

 50 mg/ml (5%) : V1 x C1 = V2 x C2
: 10 ml x 50 mg/ml = V2 x 60 mg/ml
V2 = 500 mg/60 mg/ml
V2 = 8,33 ml

 40 mg/ml (4%) : V1 x C1 = V2 x C2
: 10 ml x 40 mg/ml = V2 x 50 mg/ml
V2 = 400 mg/ 50 mg/ml
V2 = 8 ml

 30 mg/ml (3%) : V1 x C1 = V2 x C2
: 10 ml x 30 mg/ml = V2 x 40 mg/ml
V2 = 300 mg/ 40 mg/ml
V2 = 7,5 ml

 20 mg/ml (2%) : V1 x C1 = V2 x C2
: 10 ml x 20 mg/ml = V2 x 30 mg/ml
V2 = 200 mg/ 30 mg/ml
V2 = 6,66 ml

 10 mg/ml (1%) : V1 x C1 = V2 x C2
: 10 ml x 10 mg/ml = V2 x 20 mg/ml
V2 = 100 mg/ 20 mg/ml
V2 = 5 ml

## 9 ml 8,88ml 8,75ml 8,57ml 8,33ml 8 ml 7,5ml 6,66ml 5 ml

10% 9% 8% 7% 6% 5% 4% 3% 2% 1%

## Universitas Sumatera Utara

48

 9 mg/ml (0,9%) : V1 x C1 = V2 x C2
: 10 ml x 9 mg/ml = V2 x 10 mg/ml
V2 = 90 mg/ 10 mg/ml
V2 = 9 ml

 8 mg/ml (0,8%) : V1 x C1 = V2 x C2
: 10 ml x 8 mg/ml = V2 x 9 mg/ml
V2 = 80 mg/ 9 mg/ml
V2 = 8,88 ml

 7 mg/ml (0,7%) : V1 x C1 = V2 x C2
: 10 ml x 7 mg/ml = V2 x 8 mg/ml
V2 = 70 mg/ 8 mg/ml
V2 = 8,75 ml

 6 mg/ml (0,6%) : V1 x C1 = V2 x C2
: 10 ml x 6 mg/ml = V2 x 7 mg/ml
V2 = 60 mg/ 7 mg/ml
V2 = 8,57 ml

 5 mg/ml (0,5%) : V1 x C1 = V2 x C2
: 10 ml x 5 mg/ml = V2 x 6 mg/ml
V2 = 50 mg/ 6 mg/ml
V2 = 8,33 ml

 4 mg/ml (0,4%) : V1 x C1 = V2 x C2
: 10 ml x 4 mg/ml = V2 x 5 mg/ml
V2 = 40 mg/ 5 mg/ml
V2 = 8 ml

 3 mg/ml (0,3%) : V1 x C1 = V2 x C2
: 10 ml x 3 mg/ml = V2 x 4 mg/ml
V2 = 30 mg/ 4 mg/ml
V2 = 7,5 ml

## Universitas Sumatera Utara

49

 2 mg/ml (0,2%) : V1 x C1 = V2 x C2
: 10 ml x 2 mg/ml = V2 x 3 mg/ml
V2 = 20 mg/ 3 mg/ml
V2 = 6,66 ml

 1 mg/ml (0,1%) : V1 x C1 = V2 x C2
: 10 ml x 1 mg/ml = V2 x 2 mg/ml
V2 = 10 mg/ 2 mg/ml
V2 = 5 ml

## 5ml 6,66ml 7,5ml 8ml 8,33ml 8,57ml 8,75ml 8,88ml

NaOCl 0,5% 0,1% 0,2% 0,3% 0,4% 0,5% 0,6% 0,7% 0,8% 0,9% 20%

## Universitas Sumatera Utara

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LAMPIRAN 2

Tabel Hasil Pengukuran Zona Hambat Esktrak Daun Sirih Hijau terhadap Pertumbuhan Streptococcus mutans

## Ekstrak Daun Sirih Hijau

Pengulangan Konsentrasi
20% 10% 9% 8% 7% 6% 5% 4% 3% 2% 1%
(mm) (mm) (mm) (mm) (mm) (mm (mm) (mm) (mm) (mm) (mm)
1 13.9 11.15 10.5 9.9 9.4 9.25 8.1 7.6 7.45 7.25 7.13
2 13.77 11.3 10.65 10.15 9.85 9.25 8.6 7.7 7.5 6.9 6.9
3 13.86 11.35 10.35 9.8 9.2 9.1 8.1 7.8 7.5 7.0 6.75
4 13.82 11.5 10.55 10.1 9.6 9.4 8.6 7.9 7.25 7.13 6.85
5 13.74 11.2 10.4 10.2 9.75 9.4 8.4 7.7 7.25 7.13 7.0

## Ekstrak Daun Sirih Hijau

Pengulangan Konsentrasi
0,9% 0,8% 0,7% 0,6% 0,5% 0,4% 0,3% 0,2% 0,1% K (+) K (-)
(mm) (mm) (mm) (mm) (mm (mm) (mm) (mm) (mm) (mm) (mm)
1 0 0 0 0 0 0 0 0 0 8.32 0
2 0 0 0 0 0 0 0 0 0 8.21 0
3 0 0 0 0 0 0 0 0 0 8.27 0
4 0 0 0 0 0 0 0 0 0 8.36 0
5 0 0 0 0 0 0 0 0 0 8.24 0

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## ONEWAY VAR00002 BY VAR00001 /STATISTICS DESCRIPTIVES HOMOGENEITY /PLOT

MEANS /MISSING ANALYSIS /POSTHOC=LSD ALPHA(0.05).

Oneway

[DataSet0]

Descriptives

VAR00002

52

53

Descriptives

VAR00002

Minimum Maximum

9% 10.35 10.65

8% 9.80 10.20

7% 9.20 9.85

6% 9.10 9.40

5% 8.10 8.60

4% 7.60 7.90

3% 7.25 7.50

2% 6.90 7.25

1% 6.75 7.13

54

VAR00002

8.772 21 88 .000

ANOVA

VAR00002

55

## Post Hoc Tests

Multiple Comparisons

VAR00002

LSD

## 95% Confidence Interval

(I) (J)
VAR00 VAR00 Mean Difference
001 001 (I-J) Std. Error Sig. Lower Bound Upper Bound

*
20% 10% 2.49800 .07191 .000 2.3551 2.6409

## Universitas Sumatera Utara

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*
0,2% 13.81800 .07191 .000 13.6751 13.9609

## K (-) 13.81800* .07191 .000 13.6751 13.9609

*
10% 20% -2.49800 .07191 .000 -2.6409 -2.3551

57

## Universitas Sumatera Utara

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*
6% .75000 .07191 .000 .6071 .8929

## K (-) 10.03000* .07191 .000 9.8871 10.1729

*
7% 20% -4.25800 .07191 .000 -4.4009 -4.1151

## Universitas Sumatera Utara

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*
2% 2.47800 .07191 .000 2.3351 2.6209

## K (-) 9.56000* .07191 .000 9.4171 9.7029

*
6% 20% -4.53800 .07191 .000 -4.6809 -4.3951

## Universitas Sumatera Utara

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*
0,7% 9.28000 .07191 .000 9.1371 9.4229

## K (-) 9.28000* .07191 .000 9.1371 9.4229

*
5% 20% -5.45800 .07191 .000 -5.6009 -5.3151

## Universitas Sumatera Utara

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*
0,3% 8.36000 .07191 .000 8.2171 8.5029

## Universitas Sumatera Utara

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*
K (-) 7.74000 .07191 .000 7.5971 7.8829

*
3% 20% -6.42800 .07191 .000 -6.5709 -6.2851

## K (-) 7.39000* .07191 .000 7.2471 7.5329

*
2% 20% -6.73600 .07191 .000 -6.8789 -6.5931

## Universitas Sumatera Utara

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*
8% -2.94800 .07191 .000 -3.0909 -2.8051

## K (-) 7.08200* .07191 .000 6.9391 7.2249

*
1% 20% -6.89200 .07191 .000 -7.0349 -6.7491

## Universitas Sumatera Utara

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*
4% -.81400 .07191 .000 -.9569 -.6711

## K (-) 6.92600* .07191 .000 6.7831 7.0689

*
0,9% 20% -13.81800 .07191 .000 -13.9609 -13.6751

65

66

## K (-) .00000 .07191 1.000 -.1429 .1429

*
0,7% 20% -13.81800 .07191 .000 -13.9609 -13.6751

## Universitas Sumatera Utara

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*
K (+) -8.28000 .07191 .000 -8.4229 -8.1371

## K (-) .00000 .07191 1.000 -.1429 .1429

*
0,6% 20% -13.81800 .07191 .000 -13.9609 -13.6751

## K (-) .00000 .07191 1.000 -.1429 .1429

*
0,5% 20% -13.81800 .07191 .000 -13.9609 -13.6751

## Universitas Sumatera Utara

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*
9% -10.49000 .07191 .000 -10.6329 -10.3471

## K (-) .00000 .07191 1.000 -.1429 .1429

*
0,4% 20% -13.81800 .07191 .000 -13.9609 -13.6751

## Universitas Sumatera Utara

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*
5% -8.36000 .07191 .000 -8.5029 -8.2171

## K (-) .00000 .07191 1.000 -.1429 .1429

*
0,3% 20% -13.81800 .07191 .000 -13.9609 -13.6751

## Universitas Sumatera Utara

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*
1% -6.92600 .07191 .000 -7.0689 -6.7831

71

## K (-) .00000 .07191 1.000 -.1429 .1429

*
0,9% 20% -13.81800 .07191 .000 -13.9609 -13.6751

72

## K (-) .00000 .07191 1.000 -.1429 .1429

*
0,8% 20% -5.53800 .07191 .000 -5.6809 -5.3951

## K (-) 8.28000* .07191 .000 8.1371 8.4229

*
K (-) 20% -13.81800 .07191 .000 -13.9609 -13.6751

## Universitas Sumatera Utara

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*
10% -11.32000 .07191 .000 -11.4629 -11.1771

74

Means Plots

75

## !..EMBAGA ILMU PENGETAHUAN INDONESIA

( lr-donesian Institute of Sciences )
PUSAT PENELITIAN BIOLOGI
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