Agilent Technologies has taken its Agilent has been developing robust
world leading LC system and made and reliable LC systems for over
it even more flexible, smarter and 30 years. The Agilent 1200 Series
ultimately equipped for the future. represents the latest advance in
this evolution of excellent integrated
The new Agilent 1200 Series is finely solutions, designed to help you
tuned to give you a winning combination achieve uncompromised data
of speed, resolution and sensitivity, quality and higher productivity.
while the modular design ensures
that you get a configuration ideally Make a secure investment in the
suited to meet your application future of your lab with the scalable
requirements. and open architecture of the Agilent
1200 Series.
3
Sensitivity and Performance
Optimized to give you complete confidence in your results
The seamless integration of each Mass selective detection HPLC-Chip/MS for reliable
module gives you unparalled accuracy for more confidence high sensitivity nanospray
and precision for both quantitative and
qualitative analysis. Highly sensitive By providing molecular weight The easy-to-use HPLC-Chip/MS
detectors deliver the lowest possible and structural information, which system significantly improves your
detection limits for even the most complements the spectral information nanoflow separations by eliminating
demanding applications. from a diode array detector, the Agilent all post-column dead volumes and
6000 Series LC/MS systems are the is ideally suited for high sensitivity
ideal solutions for scientists who need nanospray LC/MS with limited
Spectral information from additional selectivity and sensitivity sample amounts.
190 to 950 nm for their chromatographic systems.
4
Superior injection precision High resolution chromatography
and lowest carryover reveals more
The selection of different autosamplers Small particle size and long separation
provides for precision levels of columns are key for higher efficiency
< 0.5 % RSD over an injection volume and resolution. The Agilent 1200
range from 0.01 µL to 2000 µL. Series Rapid Resolution system fully
The unique flow-through design, exploits the power of Agilent ZORBAX
combined with the external needle Rapid Resolution HT columns with
wash and optional multiple wash 1.8 µm particles, providing a new
solvents, virtually eliminates carryover, dimension of information for complex
even for highly adsorptive compounds. samples.
By choosing a system from the fast Agilent 1200 Series Fast cycle time for
quaternary/binary LC system through binary pump SL ultra-fast analysis
to the ultra-fast rapid resolution
system you can achieve new levels of • Configurable delay volume down • High performance autosamplers
productivity, processing more samples to 120 µL together with a flow enable fast injection cycles without
in less time, while maintaining and range up to 5 mL/min provides sacrificing lowest carryover
even improving data quality. universal applicability
• Valve solutions for alternating
Short columns with sub-2 µm • Electronic damping control column regeneration
particles offer a unique opportunity for lowest baseline noise
• Minimized run-to-run instrument
to dramatically reduce analysis time • Perfect choice for fast and and software initialization time
by increasing the flow rate without precise gradients using LC/MS,
losing separation performance. as well as UV-only systems
In order to take full advantage of
their power, you need a system that • Fully exploits the speed and
can do more than handle high back separation potential of ZORBAX
pressure. The answer is a fully Rapid Resolution HT columns
integrated solution, optimized
for system volumes, cycle times,
detection rates and temperature
control. mAU 0.246 - Butyrophenone
0.139 - Acetophenone
350
0.088 - Acetanilide
0.196 - Propiophenone
0.290 - Valerophenone
300
0.264 - Benzophenone
0.364 - Heptanophenone
0.330 - Hexanophenone
0.394 - Octanophenone
200
Column: ZORBAX SB RRHT C18,
2.1 mm x 50 mm, 1.8 µm
Flow = 2.4 mL/min 150
Gradient: water/acetonitrile
0 min 35 %
0.38 min 95 % 100
0.46 min 95 %
0.47 min 35 %
Temperature: 95 °C 50
Pressure: 550 bar
Detection: DAD SL, 80 Hz data rate
0
Ultra-fast separation of a phenone
mix with the Agilent 1200 Series
Rapid Resolution system. 0.1 0.2 0.3 0.4 min
6 12 18 24 s
6
Complete ZORBAX Rapid High temperature enables Ultra-fast detectors with
Resolution HT column portfolio higher speed uncompromised sensitivity
The optimized particle size distribution The two independently controllable • Programmable variable wavelength
results in less back pressure which low volume heat exchangers detector VWD (55 Hz)
makes these columns the number one allow ultra-fast, high temperature
• Multiple wavelength detector
choice for all LC systems. separation with unique post-column
MWD (80 Hz)
cooling for the lowest possible
UV detection limits. • Diode array detector for full
spectral acquisition DAD (80 Hz)
• Five types of mass spectrometers,
all with fast spectral acquisition
that can keep up with the fastest
chromatography
With an installed base of more than Robust components Agilent system intelligence
60,000 LC systems, Agilent LC
technology is renowned for its • Long-life pistons and seals, • Early Maintenance Feedback (EMF)
reliability under all operating together with active seal wash, tracks system usage (such as lamp
conditions. The Agilent 1200 Series reduce the frequency of preventive burn time, solvent usage) and alerts
represents the next generation of LC, maintenance you for timely replacement of parts
designed for even greater robustness • Deuterium and xenon lamps with • Column identification module
and reliability together with enhanced a lifetime of over 2000 hours records column parameters (such
performance. as number of injections, particle
• Patented orthogonal spray design
Agilent pioneered technologies to size and maximum pressure) and
substantially reduces ion source
monitor and track important system provides a unique column signature
contamination and the need for
parameters with the goal of ensuring cleaning • Patented RFID tracking technology
that you achieve greater productivity in the DAD SL saves all relevant
and keep operating costs low. Easy maintenance meta data from the flow cells
• Demonstration videos on a and UV lamp
multimedia CD-ROM guide you • Control Area Network (CAN)
through maintenance procedures provides inter-modular, real-time
• Easily interchangeable ion sources communication for reliable
operation, regardless of PC
• E-Pac design significantly reduces
failures or network interruptions
assembly and disassembly time
• Software independent LC diagnostic
tool assists users and the support
experts interpreting the instrument
status
• Agilent system intelligence provides
a new generation of services with
on-demand remote connectivity
for a new level of productivity
8
Customer support and services
Agilent provides customer-focused
products and services for all stages
in an instrument's life cycle, from
installation and upgrade to operation
and repair. The Agilent global network
of support services offers a broad
range of options for customized or
contract services, enabling you to
choose the package which best suits
your laboratory needs and budget
requirements.
Agilent
Your Laboratory Network Support
Infrastructure
In parallel to the development of ChemStation for research and EZChrom Elite for maximum
enhanced analytical hardware, method development tasks flexibility and compliance
Agilent develops software solutions
designed to meet your specific needs. Agilent ChemStation enables you Agilent EZChrom Elite workstation
The Agilent range extends from to acquire, review and organize large is an easy to use software solution
single workstations through to client- amounts of data, giving you instant for labs with instruments from
server solutions and an innovative access to online instrument multiple vendors. It also provides
informatics framework to unite your modifications and results. The built-in high automation capability with
enterprise, ensuring that you can macro language makes it easy to advanced “smart” sequencing and
expand your operations easily customize and the software supports reporting capabilities with automated
and effectively. numerous add-ons. spreadsheet calculations.
Workstation software systems • Level 5 instrument control for your • Full compliance features
Agilent instruments
to improve your daily output • Integrated multi-vendor instrument
• Built-in diagnostics, maintenance control
Agilent workstations give you high
and Early Maintenance Feedback
level instrument control, data • Powerful and flexible reporting
(EMF) functions for maximum
acquisition and data management • SMART sequencing for flexible
uptime
capabilities, designed to help you automation tasks
increase productivity. All software • Control of LC/MS, GC/MS, CE,
systems are available with Installation CE/MS and generic A/D converters • Easy scale-up from workstation
Qualification (IQ) and Operational to client-server system
• Add-on modules for specific
Qualification (OQ). solutions, such as high throughput
• Reduce training costs purification software, GPC/SEC
Data Analysis, Analyst, LC/MS Easy
• Comply with regulatory guidelines
Access, ChemStation Data Browser
• Extend the system as lab needs grow
• Employ sophisticated level 4
and 5 instrument control
Color-coded
information on status
Parameters can be
of 1200 Series system
changed immediately
Signal plot(s) can be
Online tip-tool enlarged to full screen
shows limits instantly
for ease of use
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Agilent OL ECM reaches Client-server systems expand
beyond the single laboratory your operations
The Agilent OL philosophy lets you As your facility grows, EZChrom Elite
communicate beyond the boundaries workstations can be easily scaled up
of a single lab. By implementing to client-server systems, enabling you
Agilent OL’s core component – to access and control your instruments
the Enterprise Content Management from any PC within the network.
system (ECM) – you can acquire and If you are working in a highly regulated
organize your data across laboratories environment, Agilent Cerity for
and departments. Pharmaceutical QA/QC provides
full workflow support of quality
• Saves all documentation and data
control operations.
in a single repository
• Organize and retrieve data
using advanced search engines
• Enables several people to rapidly
and easily review a complete
result set for a sample, including
graphical results
12
13
Module Selection Guide
Agilent 1200 Series pumping systems * settable flow range with degasser
www.agilent.com/chem/1200pumps
Agilent interfaces
14
Agilent 1200 Series injection systems
Manual Injectors Standard Autosampler Micro Well-plate Autosampler High Performance Autosampler
Injection range: Injection range: 0.1 µL–100 µL Injection range: 0.01–8 µL Injection range: 0.1 µL–100 µL
loops for 5 µL–20 mL (extendable up to 5000 µL) (extendable up 40 µL) (extendable up to 1500 µL)
Sample container: vials Sample container: Sample container: vials and
vials and well-plates well-plates
High Performance Autosampler SL Dual Loop Autosampler PS Preparative Autosampler Thermostat for Autosampler
Injection range: 0.1 µL–100 µL Injection range: up to 10 mL Injection range: 0.1–5000 µL Temperature range: 4–40 °C
(extendable up to 1500 µL) Sample container: vials and Sample container: vials
Sample container: vials and well-plates
well-plates
www.agilent.com/chem/1200autosamplers
Micro Collector/Spotter Fraction Collector (AS) Fraction Collector (PS) Thermostat for Fraction Collector
Flow rate: up to 100 µL/min Flow rate: up to 10 mL/min Flow rate: up to 100 mL/min Temperature range: 4–40 °C
15
All the performance.
Variable Wavelength Detector Variable Wavelength Detector SL Multiple Wavelength Detector Multiple Wavelength Detector SL
for programmable single ë analysis, for ultra-fast, programmable single ë for multi-ë analysis for ultra-fast multi-ë analysis
1 signal, 13 Hz data rate analysis, 1 signal, 55 Hz data rate 5 signals, 20 Hz data rate 8 signals, 80 Hz data rate
Diode Array Detector Diode Array Detector SL Fluorescence Detector Refractive Index Detector
for multi-ë and spectral analysis for ultra-fast multi-ë and spectral for multi-signal detection and for refractive index range:
5 signals, 20 Hz data rate analysis, 8 signals, 80 Hz data rate online fluorescence spectra 1.00–1.75 calibrated
www.agilent.com/chem/1200detectors
2-position/10-port valve 2-position/10-port micro valve 2-position/6-port valve 2-position/6-port micro valve
Controllers
Agilent ChemStation
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All the time.
Agilent 6100 Series Single Quadrupole Agilent 6410 Triple Quadrupole LC/MS Agilent 1200 Series HPLC-Chip/MS
LC/MS Systems Accurate MS/MS quantification with nanospray source
Nominal mass detection
Agilent 6210 Time-of-Flight LC/MS Agilent 6300 Series Ion Trap Agilent 6510 Quadrupole Time-of-Flight LC/MS
Ultra-fast accurate mass LC/MS Systems MS/MS structural information
Sensitive MSn with accurate mass
Ionization sources
Pure
LC
Speed and resolution
HPLC-Chip/MS System
Nanoflow LC System
Capillary LC System
Preparative LC Systems
Sensitivity
Highest sensitivity detection, especially • Electronic Flow Control (EFC)
in combination with limited sample measures and actively controls
amounts, is a challenge in applications, the flow in real time for highest
such as proteomics and drug retention time stability
metabolism studies. At constant • Dedicated, thermostatted micro
injection volumes, decreasing the autosamplers designed for
column inner diameter creates reproducible injection of small
smaller elution volumes. This results sample amounts, as low as 10 nL
in higher concentrations, as well
as higher signals in concentration • Theoretical sensitivity r2
= 12
sensitive detectors, such as UV increase r2
absorbance or electrospray LC/MS. r = column radius
Sensitivity
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Automated Quaternary LC System Automated Binary LC System Rapid Resolution LC System
For routine method development For research, high-throughput Highest speed and resolution with
and high throughput gradient and fast applications uncompromised data quality
applications
• High pressure gradient mixing • Up to 60 % more resolution
• Greatest flexibility for multi- for superior gradient performance and up to 20 times faster
solvent gradients by accessing even at lower flow rates compared to conventional LC
up to 4 solvents • Flow rates up to 5 mL/min
• Optimized delay volumes for
• Flow rates up to 10 mL/min fast chromatography provide universal applicability
support both standard bore • Full, uncompromised compatibility
• Flow rates from 0.05 to 5 mL/min
and semi-preparative applications with existing methods
provides ideal support for narrow
• Wide selection of autosamplers and standard bore applications • Ideally suited for use with
for full flexibility in injection ZORBAX Rapid Resolution HT
volumes and sample containers 1.8 µm columns
• Throughput of 2000 samples per day
Purification System
• Automated delay volume calibration
• Integrated safety features
• Software solutions for different
user needs
• Smart fraction collection based
on time, peak and mass, or both
• Individual column performance • Broad selectivity choices for • Lengths from 20 – 150 mm for high
reports to document column-to- enhanced resolution with six speed and high resolution analyses
column and lot-to-lot reproducibility different bonded phases (two
• 60% resolution improvement over
Eclipse Plus, four Eclipse XDB)
• Superior particle strength for even conventional LC
for the column that is best
the most demanding high pressure for your separation needs • ZORBAX RRHT columns are
applications available in 8 different bonded
• Quick and seamless method
phases
transfer from analytical to prep
and to fast and ultra-fast LC with
particle sizes from 1.8 to 7 µm
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1200_SP_FINAL_240608_complete.qxp 10.11.2008 10:25 Seite 24
www.agilent.com/chem/1200
© Copyright 2006-2008 Agilent Technologies
Printed in Germany, November 1, 2008
Publication Number 5989-5200EN