Liver enzyme lab

Hypostasis

- If normal liver contact with room temperature H202 the bubble that is came up from the liver will
be in normal amount.
- If cold liver contact with room temperature H202 the bubble that is came up from the liver will be
in small amount.
- If heated liver contact with room temperature H202 the bubble that is came up from the liver will
be in big amount.
- If acidified liver contact with room temperature H202 the bubble that is came up from the liver will
be in big amount.
- If basified liver contact with room temperature H202 the bubble that is came up from the liver will
be in small amount.

Result

The reaction will happen best in the cold temperature but worst at hot temperature. It reaction is
better in base then acid.

Hypothesis

Condition
Normal liver + Room temp H202
Cold liver + room temp H202
Heated liver + room H202
Acidified liver + room temp H202
Basified liver + room temp H202
Hypothesis
If the liver contact with the H202 the bubble will
be release in normal amount.
If the liver contact with the H202 the bubble will
be release in small amount.
If the liver contact with the H202 the bubble will
be release in high amount.
If the liver contact with the H202 the bubble will
be release in high amount.
If the liver contact with the H202 the bubble will
be release in small amount.

Result

Least reaction = 1 , normal reaction = 2 , high reaction = 3

Condition Rating of bubble

Normal liver + Room temp H202 3
Cold liver + room temp H202 3
Heated liver + room H202 1
Acidified liver + room temp H202 1
Basified liver + room temp H202 2
 Condition Time lapse
Normal liver + Room temp H202 13.46 min
Cold liver + room temp H202 15.13 min
Heated liver + room H202 0
Acidified liver + room temp H202 0
Basified liver + room temp H202 5.43 min

The result is when the temperature is too high it will denature and if the acid and is too high it will not
cause the reaction. My hypothesis is all wrong I thought that if the acid and the temperature is high the
reaction will happen faster but the result is when the low temperature and the basified can cause the
enzyme reaction happen easier. The reaction happened best when the H202 contact with low
temperature and base liver. Catalase is reusable because catalase is just a things that speed up reaction.
This test is saying to us that it is catabolic because the reaction release the heats to the tube. I think the
errors that I found in the lab is the liver is not cold and heat enough . I think I have to heat and freeze
the liver even more.

Conclusion

The reaction will happen best in the cold liver basified liver and normal liver but work worst in the
heated and acidified liver.

Reference

https://www.khanacademy.org/science/high-school-biology/hs-energy-and-transport/hs-enzymes/a/hs-
enzymes-review