Microbiologics Information Manual-9020-7751 PDF

MICROBIOLOGY
MicroScan
Microbiologics
Information Manual
9020-7751, Rev. A
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Software screen illustrations within this manual are representational only and may not exactly match the
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The information in this guide was correct at the time of printing. However, Beckman Coulter continues to
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procedures at any time without notice. Because variance cannot be precluded entirely, we cannot
guarantee full consistency. The information in this publication is reviewed regularly and necessary
corrections are included in subsequent editions.
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Regulatory information
For in vitro Diagnostic Use
Revision history
Document Date Revisions

9020-7751 Rev. A 07/2015 New document
9020-7751, Rev. A
07/2015
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© 2015 Beckman Coulter, Inc. All rights reserved. Spec: 9900-3929

Contents
Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
1 Rapid Neg ID 3 panels: Gram-negative aerobic bacilli . . . . . . . . . . . . . . . . . . . . . . . . . .11

Taxa in database . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Taxa for gram-negative fermenters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Table 1-A: Gram-negative fermenters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Taxa for gram-negative nonfermenters 19
Table 1-B: Gram-negative nonfermenters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Supplemental information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Special characteristics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Table 1-C: Special characteristics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Footnotes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Additional tests . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
Table 1-D: Additional tests . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
Tests included on Rapid Neg panels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Table 1-E: Tests included on Rapid Neg panels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
2 Dried (Conventional) Pos panels: Gram-positive aerobic bacteria . . . . . . . . . . . . . . . .33

Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
Taxa in database . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
Taxa for Staph/related genera (Micrococcaceae) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
Table 2-A: Staph/related genera (Micrococcaceae) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
Taxa for Streptococcacae . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Table 2-B: Streptococcaecae . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Gram-positive taxa/group level identifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
Table 2-C: Gram-positive taxa . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
Group-level identifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
Table 2-D: Group-level identifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
Footnotes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
MicroScan Microbiologics Information Manual 9020-7751 Rev. A

4 Contents
Additional tests for separation of Staph/related genera (Micrococcaceae) . . . . . . . . . . . . . . . . . . . . . . 46
Table 2-E: Additional tests for separation of Staph/related genera (Micrococcaceae) . . . . . . . . . . . . . . 46
Additional tests for separation of catalase negative gram-positive cocci . . . . . . . . . . . . . . . . . . . . . . . 48
Table 2-F: Additional tests for separation of catalase negative gram-positive cocci . . . . . . . . . . . . . . . . 48
Additional tests for separation of non-β hemolytic streptococci and other gram-positive cocci . . . . 49
Table 2-G: Additional tests for separation of non-β hemolytic streptococci and other gram-positive cocci 49
Additional tests for separation of β hemolytic streptococci . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
Table 2-H: Additional tests for separation of β hemolytic streptococci . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
Additional tests for separation of enterococci . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
Table 2-I: Additional tests for separation of enterococci . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
Tests included on Dried (Conventional) Pos panels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
Included tests for Staph/related genera (Micrococcaceae) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
Included tests for Streptococcaceae . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
Principles of identification reactions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54
3 Dried (Conventional) Neg panels: Gram-negative aerobic bacilli . . . . . . . . . . . . . . . . .57

Taxa in database . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
Gram-negative glucose fermenters 58
Table 3-A: Gram-negative glucose fermenters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
Gram-negative glucose nonfermenters/slow glucose fermenters 61
Table 3-B: Gram-negative glucose nonfermenters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
Footnotes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
Table 3-C: Additional tests for Gram-negative glucose fermenters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71
Table 3-D: Additional tests for Gram-negative glucose nonfermenters/slow glucose fermenters . . . . . . 73
Tests included on Dried (Conventional) Gram-negative panels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
Table 3-E: Included tests for Gram-negative glucose fermenters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
Included tests for glucose nonfermenters/slow glucose fermenters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75
Table 3-F: Included tests for Gram-negative glucose nonfermenters . . . . . . . . . . . . . . . . . . . . . . . . . . . 75
Principles of identification reactions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76
4 Yeast and yeast-like microorganisms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .79

Yeast and yeast-like taxa in database . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80
Table 4-A: Yeast and yeast-like microorganisms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80
Microscopic Morphologic Characteristics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82
Table 4-B: Microscopic Morphological characteristics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82
Typical characteristics by species . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83
9020-7751 Rev. A MicroScan Microbiologics Information Manual

Contents 5
Table 4-C: Typical characteristics by species . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83

Footnotes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
Table 4-D: Additional tests for yeast and yeast-like organisms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88
Additional tests for the separation of yeasts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89
Table 4-E: Additional tests for the separation of yeasts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89
Tests included on Rapid Yeast Identification panels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91
Table 4-F: Tests included on Rapid Yeast Identification panels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91
5 Anaerobes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .93
Anaerobe taxa in database . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 94
Anaerobic cocci 94
Table 5-A: Anaerobic cocci . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 94
Clostridia 94
Table 5-B: Clostridia . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 94
Anaerobic nonspore-forming gram-negative bacilli (NSF-GNB) 95
Table 5-C: Anaerobic nonspore-forming gram-negative bacilli (NSF-GNB) . . . . . . . . . . . . . . . . . . . . . . . 95
Anaerobic nonspore-forming gram-positive bacilli (NSF-GNB) 96
Table 5-D: Anaerobic nonspore-forming gram-positive bacilli (NSF-GNB) . . . . . . . . . . . . . . . . . . . . . . . 96
Table 5-E: Special characteristics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 97
Additional identification tests for Rapid Anaerobe Identification panels . . . . . . . . . . . . . . . . . . . . . . . . 98
Table 5-F: Additional identification tests for clostridia . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 98
Anaerobic nonspore-forming gram-negative bacilli . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 99
Anaerobic cocci 99
Table 5-G: Additional identification tests for anaerobic cocci . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 99
Anaerobic nonspore-forming gram-positive bacilli 100
Table 5-H: Additional identification tests for anaerobic nonspore-forming gram-positive bacilli . . . . . . . 100
Tests included on Rapid Anaerobe Identification panels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101
Table 5-I: Additional tests . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101
6 Haemophilus - Neisseria . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .103

Haemophilus - Neisseria taxa in database . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 104
Table 6-A: Haemophilus - Neisseria . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 104
Supplemental information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105
Footnotes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105
Additional tests . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 107
Table 6-B: Additional tests . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 107
Tests included on HNID panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 108

6 Contents
Table 6-C: Tests included on HNID panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 108

HNID Additional tests for the separation of Haemophilus and Neisseria . . . . . . . . . . . . . . . . . . . . . . . 109
Table 6-D: HNID Additional tests for the separation of Haemophilus and Neisseria . . . . . . . . . . . . . . . 109
7 Vancomycin and Streptomycin Synergy Screen Susceptibility algorithm . . . . . . . . .111

Dried (Conventional) gram-positive panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 112
Susceptibility algorithm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 112
8 Panel Processing (Read Times) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .113

Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 114
About relative read times . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 114
About overtime . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 114
Rapid Gram-negative ID 3 panels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 114
Table 8-A: Rapid gram-negative ID 3 panels processing and read times . . . . . . . . . . . . . . . . . . . . . . . 114
Dried (Conventional) Gram-positive panels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 115
Table 8-B: Dried (Conventional) Gram-positive panels processing and read times . . . . . . . . . . . . . . . . 115
Dried (Conventional) Gram-negative panels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 115
Table 8-C: Dried (Conventional) Gram-negative panels processing and read times . . . . . . . . . . . . . . . 115
Notes for Dried (Conventional) Gram-positive and Gram-negative panels . . . . . . . . . . . . . . . . . . . . . . . . 116
Rapid Yeast ID panels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 117
Table 8-D: Rapid Yeast ID panels processing and read times . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 117
HNID panels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 117
Table 8-E: HNID panels processing and read times . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 117
Rapid Neg ID 4 panels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 117
Table 8-F: Rapid Neg ID 4 panels processing and read times . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 117
Rapid Pos ID 2 panels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 118
Table 8-G: Rapid Pos ID 2 panels processing and read times . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 118
MICroSTREP plus panels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 118
Table 8-H: MICroSTREP plus panels processing and read times . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 118
9 Rapid Neg ID 4 panels: Gram-negative aerobic bacilli . . . . . . . . . . . . . . . . . . . . . . . . .119

Gram-negative aerobic bacilli taxa in database . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 120
Taxa for gram-negative fermenters 120
Table 9-A: Gram-negative fermenters - Gram-negative aerobic bacilli . . . . . . . . . . . . . . . . . . . . . . . . . 120
Taxa for gram-negative nonfermenters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 128
Table 9-B: Gram-negative nonfermenters - gram-negative aerobic bacilli . . . . . . . . . . . . . . . . . . . . . . . 128
Special characteristics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 131
Table 9-C: Special characteristics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 131
Footnotes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 132

Contents 7

Table 9-D: Additional tests for Rapid Neg ID 4 panels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 139
Tests included on Rapid Neg ID 4 Neg panels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 141
Table 9-E: Tests included on Rapid Neg ID panels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 141
10 Rapid Pos ID 2 panels: Gram-positive aerobic bacteria . . . . . . . . . . . . . . . . . . . . . . .143

Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 144
Taxa in database . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 144
Taxa for Staph/related genera (Micrococcaceae) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 144
Table 10-A: Staph/related genera (Micrococcaceae) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 144
Taxa for Streptococcaceae . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 145
Table 10-B: Streptococcaceae . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 145
Rapid gram-positive taxa/group-level identifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 147
Table 10-C: Rapid gram-positive taxa . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 147
Group-level identifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 148
Table 10-D: Group-level identifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 148
Special characteristics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 150
Table 10-E: Special characteristics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 150
Footnotes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 151
Additional test table for Staph/related genera (Micrococcaceae) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 157
Table 10-F: Additional tests for the Staph/related genera (Micrococcaceae) . . . . . . . . . . . . . . . . . . . . 157
Additional test tables for Streptococcaceae . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 158
Table 10-G: Additional tests for catalase negative gram-positive cocci . . . . . . . . . . . . . . . . . . . . . . . . . 158
Non-β hemolytic streptococci . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 159
Table 10-H: Additional tests for non-β hemolytic streptococci . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 159
β Hemolytic streptococci 160
Table 10-I: β Hemolytic streptococci . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 160
Enterococci 161
Table 10-J: Enterococci . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 161
Tests included on Rapid Pos ID 2 panels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 162
Table 10-K: Tests included on Rapid Pos ID 2 panels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 162
Additional test table and footnote references . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 164
11 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .165
Probability of acceptable identification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 166
Test abbreviations and definitions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 167
Table 11-A: Test abbreviations and definitions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 167
About large format data tables . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 174
Viewing the large format data tables . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 174

8 Contents
Printing the large format data tables . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 174

Rapid Neg ID 3: Additional tests for the separation of gram-negative aerobic bacilli . . . . . . . . . . . . . 175
Additional Tests for the Separation of Gram-Negative Aerobic Bacilli . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 175
Dried Neg: Additional tests for the separation of gram-negative glucose fermenters . . . . . . . . . . . . 179
Additional tests for the separation of gram-negative glucose fermenters . . . . . . . . . . . . . . . . . . . . . . . . . . . 179
Dried Neg: Additional tests for the separation of gram-negative glucose nonfermenters/
slow glucose fermenters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 182
Additional tests for the separation of gram-negative glucose nonfermenters/slow glucose fermenters . . . . 182
Anaerobes: Additional identification tests for anaerobic nonspore-forming gram-negative bacilli . 185
Additional identification tests for anaerobic nonspore-forming gram-negative bacilli . . . . . . . . . . . . . . . . . . 185
Rapid Neg ID 4: Additional tests for the separation of gram-negative aerobic bacilli . . . . . . . . . . . . . 187
Additional tests for the separation of gram-negative aerobic bacilli . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 187

Preface
This section includes the following topic:
• Introduction (page 10)

10 Preface
Introduction
This guide provides supplementary information you can use during automated identification
testing. Each section of the manual provides information about different MicroScan panel types.
These sections include information about taxa in the database, special characteristics, and
supplemental information (including footnotes). In addition, each section provides information
about additional separation test tables (where applicable) for each group of microorganisms, and
standard tests included on MicroScan panels.
This guide includes information that supports LabPro v.4.11, v4.30, and v4.41 software and
related MicroScan panels. Contact your Beckman Coulter representative if you have specific
questions about specific software or panel compatibilities.
Who should use this guide

This guide is intended for MicroScan and WalkAway customers who work with MicroScan
panels.
Description of topics
The following sections describe MicroScan panel types:
• Rapid Neg ID 3 panels: Gram-negative aerobic bacilli (page 11)
• Dried (Conventional) Pos panels: Gram-positive aerobic bacteria (page 33)
• Dried (Conventional) Neg panels: Gram-negative aerobic bacilli (page 57)
• Yeast and yeast-like microorganisms (page 79)
• Anaerobes (page 93)
• Haemophilus - Neisseria (page 103)
• Rapid Neg ID 4 panels: Gram-negative aerobic bacilli (page 119)
• Rapid Pos ID 2 panels: Gram-positive aerobic bacteria (page 143)
The following sections include additional topics of interest to WalkAway System users.
• Vancomycin and Streptomycin Synergy Screen Susceptibility algorithm (page 111) explains
the algorithm used by the WalkAway System software to determine Vancomycin and
Streptomycin Synergy Screen susceptibility
• Panel Processing (Read Times) (page 113) details read times for all MicroScan panels read
by the WalkAway System.
• References (page 165) includes probability tables, common definitions and data tables that
apply to all panels.

1
Rapid Neg ID 3 panels:
Gram-negative aerobic bacilli
This section includes the following topics:
• Taxa in database (page 12)
• Supplemental information (page 22)
• Special characteristics (page 22)
• Footnotes (page 23)
• Additional tests (page 28)
• Tests included on Rapid Neg panels (page 30)
More information
See References (page 165) for the following large format data table:
Rapid Neg ID 3: Additional tests for the separation of gram-negative aerobic bacilli (page 175)

12 Rapid Neg ID 3 panels: Gram-negative aerobic bacilli
Taxa in database
This topic includes the following tables:
• Taxa for gram-negative fermenters (in this section)
• Taxa for gram-negative nonfermenters (page 19)
Taxa for gram-negative fermenters

Table 1-A: Gram-negative fermenters
Synonym/Other
Abbreviation Organism Designation
A. caviae cplx. Aeromonas caviae complex Aeromonas caviae
Aer hyd/trot/ver Aeromonas hydrophila complex N/A
Aeromonas trota N/A
A. jandaei Aeromonas jandaei N/A
A. schubertii Aeromonas schubertii N/A
Aeromonas sp. Aeromonas species N/A
Aeromonas caviae complex N/A
Aeromonas hydrophila complex N/A
Aeromonas jandaei N/A
Aeromonas schubertii N/A
Aeromonas trota N/A
Aeromonas veronii biovar veronii Aeromonas veronii
Aeromonas veronii biovar sobria Aeromonas sobria
Cedecea davisae Cedecea davisae N/A
Cedecea lapagei Cedecea lapagei N/A
Cedecea neteri Cedecea neteri N/A
Cedecea sp. 3/5 Cedecea species 3 N/A
Cedecea species 5 N/A

Rapid Neg ID 3 panels: Gram-negative aerobic bacilli 13
Table 1-A: Gram-negative fermenters (continued)

Synonym/Other
Cedecea sp. Cedecea species N/A
Cedecea davisae N/A
Cedecea lapagei N/A
Cedecea neteri N/A
C. violaceum Chromobacterium violaceum N/A
C. amalonaticus Citrobacter amalonaticus N/A
Cit bra/freu/sed Citrobacter braakii N/A
Citrobacter freundii complex N/A
Citrobacter sedlakii N/A
C. farmeri Citrobacter farmeri Citrobacter amalonaticus bg 1
C. koseri Citrobacter koseri Citrobacter diversus
Cit werk/youngae Citrobacter werkmanii N/A
Citrobacter youngae N/A
Citrobacter sp. Citrobacter species N/A
Citrobacter amalonaticus N/A
Citrobacter braakii N/A
Citrobacter freundii complex N/A
Citrobacter farmeri Citrobacter amalonaticus bg 1
Citrobacter koseri Citrobacter diversus
Citrobacter werkmanii N/A
C. sakazakii Cronobacter sakazakii Enterobacter sakazakii
E. tarda Edwardsiella tarda N/A
E. aerogenes Enterobacter aerogenes N/A
E. amnigenus 1 Enterobacter amnigenus 1 N/A


Synonym/Other
E. asburiae Enterobacter asburiae N/A
E. cancerogenus Enterobacter cancerogenus Enterobacter taylorae
E. cloacae Enterobacter cloacae N/A
E. gergoviae Enterobacter gergoviae N/A
E. hormaechei Enterobacter hormaechei N/A
Enterobacter sp. Cronobacter sakazakii N/A
Enterobacter species N/A
Enterobacter aerogenes N/A
Enterobacter amnigenus 1 N/A
Enterobacter cancerogenus Enterobacter taylorae
Enterobacter cloacae N/A
Enterobacter gergoviae N/A
Enterobacter hormaechei N/A
Kluyvera intermedius N/A
Pantoea agglomerans Enterobacter agglomerans
E. coli Escherichia coli N/A
E. coli O157:H7 Escherichia coli O157:H7 N/A
E. fergusonii Escherichia fergusonii N/A
E. hermannii Escherichia hermannii N/A
E. vulneris Escherichia vulneris N/A
Escherichia sp. Escherichia species N/A
Escherichia fergusonii N/A
Escherichia hermannii N/A
Escherichia vulneris N/A
E. americana Ewingella americana N/A
G. hollisae Grimontia hollisae Vibrio hollisae


Synonym/Other
Hafnia alvei Hafnia alvei N/A
K. oxytoca Klebsiella oxytoca N/A
K. ozaenae Klebsiella ozaenae N/A
K. pneumoniae Klebsiella pneumoniae N/A
K. rhinoscler Klebsiella rhinoscleromatis N/A
Klebsiella sp. Klebsiella species N/A
Raoultella ornithinolytica Klebsiella ornithinolytica
Klebsiella oxytoca N/A
Klebsiella ozaenae N/A
Klebsiella pneumoniae N/A
K. intermedia Kluyvera intermedia Enterobacter intermedius
Kluyvera sp. Kluyvera species N/A
Kluyvera ascorbata N/A
Kluyvera cryocrescens N/A
L. adecarboxy Leclercia adecarboxylata N/A
Leminorella sp. Leminorella species N/A
M. wisconsensis Moellerella wisconsensis N/A
M. morganii Morganella morganii N/A
P. aerogenes Pasteurella aerogenes N/A
P. agglomerans Pantoea agglomerans Enterobacter agglomerans
Ppneu/Aure/Mhaem Actinobacillus urea Pasteurella ureae
Mannheimia haemolytica Pasteurella haemolytica
Pasteurella pneumotropica N/A
P. multocida Pasteurella multocida N/A


Synonym/Other
Pasteurella sp. Pasteurella species N/A
Pasteurella aerogenes N/A
Actinobacillus urea Pasteurella ureae
Pasteurella multocida N/A
P. damselae Photobacterium damselae Vibrio damsela
P. shigelloides Plesiomonas shigelloides N/A
P. mirabilis Proteus mirabilis N/A
P. penneri Proteus penneri N/A
P. vulgaris Proteus vulgaris N/A
Proteus sp. Proteus species N/A
Proteus mirabilis N/A
Proteus penneri N/A
Proteus vulgaris N/A
P. alcalifaciens Providencia alcalifaciens Providencia alcalifaciens 1-2
P. rettgeri Providencia rettgeri N/A
P. rustigianii Providencia rustigianii N/A
P. stuartii Providencia stuartii N/A
Providencia sp. Providencia species N/A
Providencia alcalifaciens N/A
Providencia rettgeri N/A
Providencia rustigianii N/A
Providencia stuartii N/A
R. ornithinolyt Raoultella ornithinolytica Klebsiella ornithinolytica
S. Choleraesuis Salmonella enterica serotype Choleraesuis Salmonella choleraesuis
S. Paratyphi A Salmonella enterica serotype Paratyphi A Salmonella paratyphi A
S. Typhi Salmonella enterica serotype Typhi Salmonella typhi


Synonym/Other
S. ent arizonae Salmonella enterica ssp. arizonae Salmonella/Arizona
S. enterica Salmonella enterica Salmonella species
Salmonella enterica serotype Choleraesuis Salmonella choleraesuis
Salmonella enterica serotype Paratyphi A Salmonella paratyphi A
Salmonella enterica ssp. arizonae Salmonella/Arizona
S. ficaria Serratia ficaria N/A
S. fonticola Serratia fonticola N/A
S. liquefac Serratia liquefaciens complex Serratia liquefaciens
S. marcescens Serratia marcescens N/A
S. odorifera 1 Serratia odorifera 1 N/A
S. plymuthica Serratia plymuthica N/A
S. rubidaea Serratia rubidaea N/A
Serratia sp. Serratia species N/A
Serratia ficaria N/A
Serratia fonticola N/A
Serratia liquefaciens complex Serratia liquefaciens
Serratia marcescens N/A
Serratia odorifera 1 N/A
Serratia plymuthica N/A
Serratia rubidaea N/A
Sh boy/dys/flex Shigella boydii N/A
Shigella dysenteriae N/A
Shigella flexneri N/A
S. sonnei Shigella sonnei N/A
Tatumella Tatumella ptyseos N/A
V. alginolyticus Vibrio alginolyticus N/A


Synonym/Other
V. cholerae Vibrio cholerae N/A
Vib fluv/furn Vibrio fluvialis N/A
Vibrio furnissii N/A
V. metschnikovi Vibrio metschnikovii N/A
V. mimicus Vibrio mimicus N/A
V. parahaemolyt Vibrio parahaemolyticus N/A
V. vulnificus Vibrio vulnificus N/A
Vibrio sp. Grimontia hollisae Vibrio hollisae
Photobacterium damselae Vibrio damsela
Vibrio species N/A
Vibrio alginolyticus N/A
Vibrio fluvialis N/A
Vibrio metschnikovii N/A
Vibrio mimicus N/A
Vibrio parahaemolyticus N/A
Vibrio vulnificus N/A
Y. entero group Yersinia enterocolitica N/A
Yersinia frederiksenii N/A
Yersinia intermedia N/A
Yersinia kristensenii N/A
Y. pestis Yersinia pestis N/A
Y. pseudotb Yersinia pseudotuberculosis N/A
Y. ruckeri Yersinia ruckeri N/A
Yersinia sp. Yersinia species N/A
Yersinia pseudotuberculosis N/A
Yersinia ruckeri N/A
Y. regensburgei Yokenella regensburgei Koserella trabulsii

Taxa for gram-negative nonfermenters

Table 1-B: Gram-negative nonfermenters
Synonym/Other
Ac baum/haem Acinetobacter baumannii complex Acinetobacter baumannii
Acinetobacter haemolyticus N/A
A. lwoffii Acinetobacter lwoffii N/A
Acinetobacter sp. Acinetobacter species N/A
Acinetobacter baumannii complex N/A
Acinetobacter lwoffii N/A
Alsp/Ac. xyl/Cpau Alcaligenes species N/A
Alcaligenes faecalis Alcaligenes odorans
Achromobacter denitrificans Achromobacter xylosoxidans

ssp.denitrificans
Achromobacter xylosoxidans Achromobacter xylosoxidans

ssp.xylosoxidans
Cupriavidus pauculus Ralstonia paucula
B. bronchisept Bordetella bronchiseptica N/A
B. zoohelcum Bergeyella zoohelcum Weeksella zoohelcum
B. cepacia cplx Burkholderia cepacia complex Burkholderia cepacia
Burkholderia sp. Burkholderia species N/A
Burkholderia cepacia complex Burkholderia cepacia
B. diminuta Brevundimonas diminuta Pseudomonas diminuta
B. vesicularis Brevundimonas vesicularis Pseudomonas vesicularis
Brevundimonas sp. Brevundimonas species N/A
Brevundimonas diminuta N/A
Brevundimonas vesicularis N/A
C. indologenes Chryseobacterium indologenes Flavobacterium indologenes
Chryseobact sp. Chryseobacterium species N/A
Chryseobacterium indologenes N/A
Elizabethkingia meningoseptica Chryseobacterium

meningosepticum

Table 1-B: Gram-negative nonfermenters (continued)

Synonym/Other
D. acido/C. test Delftia acidovorans Comamonas acidovorans
Comamonas testosteroni Pseudomonas testosteroni
E. corrodens Eikenella corrodens N/A
E. menigosepti Elizabethkingia Chryseobacterium

meningosepticum
meningoseptica
E. brevis Empedobacter brevis Flavobacterium breve
Morax/Psychr spp. Moraxella species N/A
Psychrobacter species N/A
Psychrobacter phenylpyruvicus Moraxella phenylpyruvica
Moraxella osloensis N/A
Moraxella nonliquefaciens N/A
Myroides sp. Myroides species Flavobacterium odoratum
N. elon/ weav/zoo Neisseria elongata EF-4B/Neisseria weaveri
Neisseria weaveri Neisseria elongata
Neisseria zoodegmatis N/A
O. anthropi Ochrobactrum anthropi Achromobacter VD 1, 2
O. ureolytica Oligella ureolytica N/A
O. urethralis Oligella urethralis N/A
Oligella sp. Oligella species N/A
Oligella ureolytica N/A
Oligella urethralis N/A
P. yeei Paracoccus yeei (CDC group EO-2) CDC EO-2 CDC group E0-2
P. aeruginosa Pseudomonas aeruginosa N/A
Ps alcal/psalc Pseudomonas alcaligenes N/A
Pseudomonas pseudoalcaligenes N/A
Ps fluor/putida Pseudomonas fluorescens N/A
Pseudomonas putida N/A
P. luteola Pseudomonas luteola Chryseomonas luteola
P. mendocina Pseudomonas mendocina N/A

Table 1-B: Gram-negative nonfermenters (continued)

Synonym/Other
P. oryzihabitans Pseudomonas oryzihabitans Flavimonas oryzihabitans
P. stutzeri Pseudomonas stutzeri N/A
Pseudomonas sp. Pseudomonas species N/A
Pseudomonas alcaligenes N/A
Pseudomonas fluorescens N/A
Pseudomonas mendocina N/A
Pseudomonas stutzeri N/A
R. pickettii Ralstonia pickettii Burkholderia (P). picketti
R. radiobacter Rhizobium radiobacter Agrobacterium radiobacter
S. putrefaciens Shewenella putrefaciens Pseudomonas putrefaciens
S. paucimobilis Sphingomonas paucimobilis Pseudomonas paucimobilis
S. multivorum Sphingobacterium multivorum N/A
S. spiritivorum Sphingobacterium spiritivorum N/A
Sphingobact sp. Sphingobacterium species N/A
Sphingobacterium multivorum N/A
Sphingobacterium spiritivorum N/A
S. maltophilia Stenotrophomonas maltophilia Xanthomonas maltophilia
W. virosa Weeksella virosa N/A

Supplemental information
In addition to probability data, biotype numbers might also display the following supplemental
information:
• Special characteristics (in this section) (Special Char.)
• Footnotes (page 23) (FTN)
Special characteristics
Special Characteristics are unique features of an organism which might help identify a particular
species. The following table lists the special characteristics used in conjunction with the
MicroScan Rapid Gram-Negative Identification System.
Table 1-C: Special characteristics

Abbreviation Special characteristic Organism
BC Brown colony S. maltophilia
S. putrefaciens
S. spiritivorum
GP Green pigment P. aeruginosa
FO Fruity odor Myroides sp.
PO Potato odor S. odorifera 1 & 2
PP Purple pigment C. violaceum
PYP Pale yellow pigment E. meningoseptica
S. multivorum
S. spiritivorum
RP Red pigment S. rubidaea
RVP Red pigment variable S. marcescens
YBC Yellow-brown colony B. zoohelcum
W. virosa

Table 1-C: Special characteristics (continued)

YP Yellow pigment B. cepacia complex
C. indologenes
E. hermannii
C. sakazakii
E. vulneris
S. paucimobilis
YSC Yellow spreading colony Myroides sp.
YWC Yellow wrinkled colony P. luteola
P. oryzihabitans
P. stutzeri
WSC White spreading colony Alcaligenes sp.
Footnotes
One or more of the footnotes listed below might be displayed to the right of the biotype number in
the FTN column.
NOTE:
Disregard footnotes A, C, D, E, G, H, K, O, P, T, U, AA, BB, and DD as they are NOT applicable
for Rapid gram-negative panels.
B. Proteus mirabilis usually have MICs of <8 mcg/mL to ampicillin,Proteus vulgaris and
Morganella morganii usually have MICs of >8 mcg/mL to ampicillin.
F. Pseudomonas aeruginosa usually demonstrates a green pigment. A. xylosoxidans is

colorless.

J. This taxon includes Pseudomonas stutzeri, Pseudomonas mendocina and CDC group VB-3.
These species may be differentiated as follows:
Wrinkled
Organism Starch Arginine colonies
P. stutzeri + - +
P. mendocina - + -
CDC VB-3 + + +
L. Confirm the identification of this organism with the special characteristics and/or additional
tests listed.
M. Serological confirmation of the identification is recommended.
N. Most hemolytic strains of Acinetobacter species, whether glucose positive or glucose-

negative, belong to Genomospecies 4, and are now designated Acinetobacter haemolyticus.
Q. Yersinia enterocolitica group includes Y. enterocolitica, Y. frederiksenii, Y. intermedia, Y.

kristensenii. These species may be differentiated as follows:
Setup a new dried overnight panel at 25°C
Organism Rhamnose Raffinose Sucrose

Y. enterocolitica - - +
Y. frederiksenii + - +
Y. intermedia + + +
Y. kristensenii - - -

Or, setup offline tests at 25°C
Organism RHA Salicin MEL SUC

Y. enterocolitica 1 20 1 95
Y. frederiksenii 99 9 20 100
Y. intermedia 100 100 80 100
Y. kristensenii 0 15 0 0
R. Yersinia pestis infections are rare in the United States. Endemic areas include New Mexico,
Arizona, California, and Colorado. If colony morphology, specimen source, and your location
in an endemic area suggest a Yersinia pestis infection, it is important to use necessary safety
precautions as well as contact your State Health Department and the physician immediately.
S. Pseudomonas fluorescens/putida includes Pseudomonas fluorescens and Pseudomonas

putida. These species may be differentiated by the following tests:
Lecithinase(Egg Yolk
Organism OF Mannitol Agar 25°C)
P. fluorescens 93 91
P. putida 19 0
V. D. acido/C. test includes Delftia acidovorans and Comamonas testosteroni.

These species may be differentiated by the following tests:
Organism OMT OF Fructose

D. acidovorans 100 100
C. testosteroni 0 0
X. Morax/Psychr spp includes: Moraxella osloensis, Moraxella nonliquefaciens and

Psychrobacter phenylpyruvicus. These species may be differentiated by the following tests:
Organism Urea Sodium acetate alkalinization

M. osloensis 0 100
M. nonliquefaciens 0 0
P. phenylpyruvicus 100 43

Y. Ps alcal/psalc includes Pseudomonas alcaligenes and Pseudomonas pseudoalcaligenes.

Organism Growth at 42C Acid from Fructose

P. alcaligenes 0 0
P. pseudoalcaligenes 94 100
Z. Kluyvera sp. includes Kluyvera ascorbata and Kluyvera cryocrescens. These species may
be differentiated by the following tests:
Organism LYS Ascorbate Test (48 hrs) Acid from Dulcitol

K. ascorbata 100 97 45
K. cryocrescens 50 0 0
EE. Leminorella sp. includes Leminorella grimontii and Leminorella richardii. These species may
Organism MR CIT
L. grimontii 100 100
L. richardii 0 0
FF. N. elon/weav/zoo includes Neisseria elongata, Neisseria weaveri and Neisseria

zoodegmatis. These species may be differentiated by the following tests:
Organism ARG GLU CAT

N. elongata 0 23 0
N. weaveri 0 0 100
N. zoodegmatis 0 100 0
GG. Vib fluv/furn includes Vibrio fluvialis and Vibrio furnissii. These species may be
differentiated by the following tests:
Organism GGL
V. fluvialis 0
V. furnissii 100

HH. Aer hyd/trot/ver includes Aeromonas hydrophila, Aeromonas trota,Aeromonas veronii

biovar veronii, and Aeromonas veronii biovar sobria. These species may be differentiated by
the following tests:
DL-
Organism ARG CEL LIpase Lactate ORN ESC
A. hydrophila 99 0 99 80 0 90
A. trota 99 99 0 88 0 0
A. veronii 0 80 90 0 99 99
biovar veronii
A. veronii 99 20 92 0 0 0
biovar sobria
II. Ppneu/Aure/Mhaem includes Pasteurella pneumotropica, Actinobacillus ureae and

Mannheimia haemolytica. These species may be differentiated by the following tests:
Organism ORN XYL URE

P. pneumotropica 100 95 96
A. ureae 0 0 100
M. haemolytica 0 100 0
JJ. Alsp/Ac. Xyl/Cpau includes Achromobacter xylosoxidans, Achromobacter denitrificans,

Alcaligenes faecalis, and Cupriavidus pauculus. These species may be differentiated by the
following tests:
Organism URE NIT XYL

A. xylosoxidans 0 100 99
A. denitrificans 0 100 0
A. faecalis 2 0 0
C. pauculus 100 11 0

KK. Ac baum/haem includes Acinetobacter baumannii complex and Acinetobacter

haemolyticus. These species may be differentiated by the following tests:
Organism Hemolysis GEL MLN

A. baumannii complex - - +
A. haemolyticus + + -
End of footnotes
Additional tests
You can use additional tests for separating organisms which appear biochemically similar.
These tests display on the right of the Special Characteristics column when the probability of the
first organism is below 85% (low probability). The identification of the unknown organism may be
any of the 5 listed strains.
LabPro software selects additional tests to assist with the identification of the top 3 choices.
The results listed for each test are expressed as percent probability of a positive result.
The following table lists the additional tests:
Table 1-D: Additional tests

Test Abbreviation Test
OXI Oxidase
GLU D-Glucose Fermentation
OGL OF Glucose
OLA OF Lactose
OMA OF Maltose
OMT OF Mannitol
OFS OF Sucrose
OXY OF Xylose
0%N Growth in 0% NaCl
10N Growth in 10% NaCl
VNI Vibrio Nitrate Reduction

Table 1-D: Additional tests (continued)

GGL Gas from D-Glucose
MR Methyl Red
VP Acetoin production (VP broth)
H2S Hydrogen Sulfide (TSI)
CIT Simmons Citrate
CUR Christensen Urea Agar
ADO Adonitol Fermentation
ARG Arginine Hydrolysis
DNA DNAse (25°C)
M36 Motility (36°C)
SUC Sucrose Fermentation
LAC Lactose Fermentation
MAL Maltose Fermentation
ARA L-Arabinose Fermentation
CEL Cellobiose Fermentation
INO Inositol Fermentation
MLN Malonate Utilization Test*
MEL Melibiose Fermentation
RAF Raffinose Fermentation
RHA Rhamnose Fermentation
SOR D-Sorbitol Fermentation
TRE Trehalose Fermentation
XYL D-Xylose Fermentation
NIT Nitrate Reduction to Nitrite
CAT Catalase
42G Growth at 42°C
FLG Flagella: Polar (PO), Peritrichous (PE), None (N)
CET Growth on Cetrimide Agar
MAC Growth on MacConkey Agar
SS Growth on Salmonella-Shigella Agar

Table 1-D: Additional tests (continued)

N2 Gas from Nitrate
GEL Gelatin Hydrolysis (22°C)
KCN Growth in Potassium Cyanide
3FL >3 Flagella
* The malonate test on the dried overnight panel cannot be substituted for
the Malonate Utilization Test.
Tests included on Rapid Neg panels
Table 1-E: Tests included on Rapid Neg panels

Identification substrates Abbreviation
α - L - Glutamate - AMC* AGLT
γ - L - Glutamic acid - AMC GGLT, GLT2
Adonitol ADN, ADN2
Arabinose ARA, ARA2
Arabitol ABT
Decarboxylase base DCB, DCB2
Glucose GLU
Glucuronic Acid GLR
Inositol INO
L - Arginine - AMC ARG
L - Proline - AMC PRO
L - Pyroglutamic acid - AMC PYG
L - Tyrosine - AMC TYR, TYR2
Lysine LYS
Mannitol MAN, MAN2
Mannose MNS
Melibiose MEL
MeU** - α - D - Glucopyranoside AGL
MeU - α - D - Galactopyranoside AGAL

Table 1-E: Tests included on Rapid Neg panels (continued)

MeU - β D - Galactopyranoside BGAL
MeU - β - D - Glucopyranoside BGL
MeU - β - D - Glucuronide BGLR
MeU - β- D - Mannopyranoside BMAN
MeU - β - D - Xyloside BXYL
MeU - β - D - N, N' - Diacetlychitobioside CHB
MeU - N-Acetyl - β - D - Glucosaminide NAG
MeU - Phosphate, Acid pH PHO1, PH1A
MeU - Phosphate, Alkaline pH PHO2
N - Glutaryl - Glycyl - Arginine - AMC GGA
Ornithine ORN, ONB
Raffinose RAF
Salicin SAL
Sorbitol SOR
Sucrose SUC
Tryptophan IND
Urea URE
* 7-Amido-4-methylcoumarin
** 4-Methylumbelliferyl
More information
• See Probability of acceptable identification (page 166) for more information.
• See Rapid Neg ID 3: Additional tests for the separation of gram-negative aerobic bacilli
(page 175) for a complete data table that shows the expected result for each test listed with
each organism in the database.

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2
Dried (Conventional) Pos panels:
Gram-positive aerobic bacteria
• Overview (page 34)
• Gram-positive taxa/group level identifications (page 37)
• Additional tests for separation of Staph/related genera (Micrococcaceae) (page 46)
• Additional tests for separation of catalase negative gram-positive cocci (page 48)
• Additional tests for separation of non-β hemolytic streptococci and other gram-positive cocci (page 49)
• Additional tests for separation of β hemolytic streptococci (page 50)
• Additional tests for separation of enterococci (page 51)
• Tests included on Dried (Conventional) Pos panels (page 52)

34 Dried (Conventional) Pos panels: Gram-positive aerobic bacteria
Overview
Gram-positive organisms are characterized on the basis of Gram morphology, colonial and
cellular morphology and the catalase test results.
The Staph/Related Genera (Micrococcaceae) section contains the catalase-positive genera

Staphylococcus, Kocuria, Micrococcus and related species, Listeria and the catalase-variable
Rothia species.
The Streptococcaceae section contains the catalase-negative genera Streptococcus,

Enterococcus, Aerococcus, Leuconostoc, Gemella, Pediococcus as well as Rhodococcus equi
(catalase positive).
Taxa in database
• Taxa for Staph/related genera (Micrococcaceae) (in this section)
• Taxa for Streptococcacae (page 35)
Taxa for Staph/related genera (Micrococcaceae)
Table 2-A: Staph/related genera (Micrococcaceae)

Synonym/Other
K. kristinae Kocuria kristinae Micrococcus kristinae
L. monocytogenes Listeria monocytogenes N/A
Micro/Rel spp. Micrococcus and Related species K. rosea
Micrococcus species
M. luteus
M. lylae
D. nishinomiyaensis
K. varians
R. dentocariosa Rothia dentocariosa N/A
R. mucilaginosa Rothia mucilaginosa N/A
S. aureus Staphylococcus aureus N/A
S. auricularis Staphylococcus auricularis N/A
S. capitis-capit Staphylococcus capitis ssp. capitis N/A

Dried (Conventional) Pos panels: Gram-positive aerobic bacteria 35
Table 2-A: Staph/related genera (Micrococcaceae) (continued)

Synonym/Other
S. capitis-urea Staphylococcus capitis ssp. urealyticus N/A
S. cohnii-cohnii Staphylococcus cohnii ssp. cohnii N/A
S. cohnii-urea Staphylococcus cohnii ssp. urealyticus N/A
S. epidermidis Staphylococcus epidermidis N/A
S. haemolyticus Staphylococcus haemolyticus N/A
S. hominis-homin Staphylococcus hominis ssp. hominis N/A
S. hominis-novo Staphylococcus hominis ssp. novobiosepticus N/A
S. hyicus Staphylococcus hyicus N/A
S. intermedius Staphylococcus intermedius N/A
S. lugdunensis Staphylococcus lugdunensis N/A
S. saprophyticus Staphylococcus saprophyticus N/A
S. schleif-coag Staphylococcus schleiferi ssp. coagulans N/A
S. shleif-schlf Staphylococcus schleiferi ssp. schleiferi N/A
S. sciuri Staphylococcus sciuri N/A
S. simulans Staphylococcus simulans N/A
S. warneri Staphylococcus warneri N/A
S. xylosus Staphylococcus xylosus N/A
Taxa for Streptococcacae

Table 2-B: Streptococcaecae
Abbreviation Organism Synonym/Other Designation
A. urinae Aerococcus urinae N/A
A. viridans Aerococcus viridans N/A
E. avium Enterococcus avium S. avium, Grp. D Enterococcus
E. casseliflavus Enterococcus casseliflavus N/A
E. durans/hirae Enterococcus durans/hirae E. durans
E. faecalis Enterococcus faecalis S. faecalis, Grp.D Enterococcus
E. faecium Enterococcus faecium S. faecium, Grp. D Enterococcus

Table 2-B: Streptococcaecae (continued)

E. gallinarum Enterococcus gallinarum N/A
E. raffinosus Enterococcus raffinosus N/A
Gemella species Gemella species N/A
Leuconostoc sp. Leuconostoc species N/A
Pediococcus sp. Pediococcus species N/A
Rhodococcus equi Rhodococcus equi N/A
S. agalact- Grp B Streptococcus agalactiae (Group B) Group B Strep.
S. anginosus grp. Streptococcus anginosus group S. milleri group
S. anginosus/milleri
S. intermedius/milleri
S. constellatus/milleri
Viridans grp. Strep.
S. bovis grp. Streptococcus bovis group Group D Strep, non-enterococcus
S. dysgal ssp. dys Streptococcus dysgalactiae ssp. Streptococcus dysgalactiae
S. equi ssp. equi Streptococcus equi ssp. equi Streptococcus equi
S. iniae Streptococcus iniae N/A
S. mitis/oralis Streptococcus mitis/oralis Viridans grp. Strep.
S. mutans Streptococcus mutans Viridans grp. Strep.
S. parasanguinis Streptococcus parasanguinis Streptococcus parasanguis
S. parasanguinis Streptococcus pneumoniae Pneumococcus
S. pyogenes Streptococcus pyogenes Group A Strep.
S. salivarius Streptococcus salivarius Viridans grp. Strep
S. sanguinis Streptococcus sanguinis Streptococcus sanguis

Gram-positive taxa/group level identifications

Gram-positive taxa
Certain species of gram-positive cocci have been combined due to similar patterns observed
with the Dried positive ID3 panel. These taxa are listed in the following table.
Some tests which may be useful for differentiation are listed in Footnotes (page 40).
Table 2-C: Gram-positive taxa

Group Includes
Micrococcus and related species Dermococcus nishinomiyaensis
Kocuria rosea
K. varians
Micrococcus luteus
M. lylae
Enterococcus durans/hirae Enterococcus durans
E. hirae
Gamella species Gamella haemolysans
G. morbillorum
Streptococcus mitis/oralis Streptococcus mitis
S. oralis
Streptococcus anginosus group Streptococcus anginosus
S. constellatus
S. intermedius
Group-level identifications
Group-level identification may be selected by the user for Staphylococci and Related Genera
and/or Streptococcaceae. If the probability of the species or taxon level identification is low, but
the sum of the probabilities of possible identifications is at least 85%, the group-level
identification will be reported if group-level identification is selected. The group-level identification

and the taxa included are listed in the following table. Note that the tests which may be useful for
differentiation are listed in footnotes or additional test tables.
Table 2-D: Group-level identifications

Group Includes
S. capitis S. capitis ssp. capitis
S. capitis ssp. urealyticus
S. cohnii S. cohnii ssp. cohnii
S. cohnii ssp. urealyticus
S. hominis S. hominis ssp. hominis
S. hominus ssp. novobiosepticus
S. schleiferi S. schleiferi ssp. schleiferi
S. schleiferi ssp. coagulans
Coagulase-negative S. auricularis
staphylococci
S. sciuri
S. simulans
S. warneri
S. xylosus
Note:
S. aureus, S. lugdunensis and S. saprophyticus are
not included in group-level ID for coagulase-negative
staphylococci because of positive coagulase
reactions or AST requirements.
Enterococcus E. avium
E. durans/hirae
E. raffinosus
C/G Streptococci S. dysgalactiae ssp. dysgalactiea
S. equi ssp. equi
S. mitis S. mitis/oralis
S. parasanguinis
S. sanguinis
Rothia R. dentocariosa
R. mucilaginosa

information:
Special characteristics are unique features of an organism which may help identify a particular
species. Below are the special characteristics used in conjunction with MicroScan Dried
(Conventional) gram-positive identification panels.
GPR Gram-positive rod L. monocytogenes
MUC Mucoid colony Rhodococcus equi
PKP Pink pigment Rhodococcus equi
SPK White/rough or spoke- wheel Rothia dentocariosa

colonies
VR Vancomycin Resistant Pediococcus sp.
Leuconostoc sp.
WTM White/transparent, sticky mucoid or Rothia mucilaginosa

adherent colonies
YP Yellow pigment E. casseliflavus

Footnotes
A. Serology may be needed for confirmation.
B. Micrococcus and Related Genera may be differentiated with the following tests:
Major Furazo- Nitrate to Gelatin

Organism pigment lidone Nitrate Hydrolysis Oxidase Urease
K. kristinae PO R - - + V
M. luteus Y R - + + V
M. lylae CW R - + + -
D. nishin O R V + + +
K. rosea PR, R + - - -
OR
K. sendentarius CW, R - + - -
BY
K. varians Y R + + - +
M. caseolyticus** - S / / + -
*Symbols: Y=yellow; CW=cream-white; PR= pastel red; OR=orange red; PO=pale orange; O=orange;
BY=buttercup yellow; /= not tested or not applicable
** Macrococcus caseolyticus are larger cocci than staphylococci or micrococci.
C. Coagulase may be a useful confirmation test. S. aureus and S. intermedius are coagulase
positive while S. hyicus and S. schleiferi are coagulase variable.
D. Anginosus group streptococci include minute-colony beta-hemolytic isolates that may type
as A, C, F, or G as well as non-beta-hemolytic strains based on phenotypic and genotypic
testing.
E. Check motility and pigment production. E. faecium is non-motile and nonpigmented. See
additional test table for more information on other enterococci.
F. This organism is commonly associated with animal sources.

H. S. mitis group includes S. mitis, S. mitis/oralis, S. parasanguinis, and S. sanguinis.
I. The S. anginosus group includes S. anginosus, S. intermedius, and S. constellatus

subspecies constellatus and pharyngis.
Organism βGAL αGLU βGLU HYA NEU

S. anginosus - V + - -
S. intermedius + + V + +
S.constellatus ssp. - + - + -
constellatus
S.constellatus ssp. + + + V -
pharyngis
Legend
βGAL: β-D-Acetylgalactosaminidase
αGLU: α - Glucosidase
βGLU: β - Glucosidase
HYA: Hyaluronidase
NEU: Neuraminidase
K. E. durans/hirae includes E. durans and E. hirae.
Organism SUC RAF

E. durans - -
E. hirae + +
Legend
SUC: Sucrose fermentation (Heart Infusion Broth)
RAF: Raffinose fermentation (Heart Infusion Broth)
P. Micrococcus and Related Species includes Dermacoccus nishinomiyaensis, Kocuria rosea,

Kocuria varians, Micrococcus luteus and Micrococcus lylae.
Organism Pigment NIT M-OX NT URE

D. nishinomiyaensis Orange V + + +
K. rosea Pale Red- + - - -
Red Orange
K. varians Yellow + - - +


M. luteus Yellow - + + V
M. lylae Cream white - + - -

Legend
NIT: Nitrate reduction to nitrite
M-OX: Modified oxidase
NT: Growth on inorganic nitrogen agar
URE: Urease
Q. Rothia species includes R. denticariosa and R. mucilaginosa.
Organism Morph ESC Colony type

R. denticariosa Rod + Spoke wheel
R. mucilaginosa Cocci + Adherent or sticky
Legend
Morph: Predominant cellular morphology on Gram stain from broth
ESC: Esculin hydrolysis
S. Gemella species includes G. haemolysans and G. morbillorum.
ALK
Organism PHOS Growth
G. haemolysans + Prefers aerobic conditions
G. morbillorum - Prefers anaerobic conditions
Legend
ALK PHOS: Alkaline phosphatase
T. Rhodococcus equi are catalase-positive pleomorphic coccobacilli. Gram stains from fluid
media frequently yield bacillary forms while coccoid forms are seen from solid media. The
characteristic mucoid coalescing colonies are generally not apparent until 48 to 72 hours.
A pale or salmon pink pigment usually develops after several days of incubation on non-
selective media. R. equi is biochemically inactive on sugars but is urease and nitrate
positive.
U. Streptococcus bovis group is a group that includes S. equinus, S. gallolyticus, S. infantarius,

and S. alactolyticus.

V. S. schleiferi ssp. schleiferi may be positive in a slide coaguase test but will be negative in a
tube coagulase test. S. schleiferi ssp. coagulans will be slide coagulase negative but tube
coagulase positive.
Slide Tube
Organism COAG COAG
S. schleiferi ssp. schleiferi + -
S. schleiferi ssp. coagulans - +
End of footnotes
Additional tests
first organism is below 85% (low probability). Up to three tests will be printed.
The results for each test are expressed as positive (+), negative (-) or variable (v) reactions.
Below is a list of the additional tests used for gram-positive aerobic bacteria on Rapid and Dried
(Conventional) panels.
Additional tests for Staph/related genera (Micrococcaceae)
Additional tests: Staph/related genera (Micrococcaceae)

Test
Abbreviation Test
ALK Alkaline phosphatase
ARG Arginine hydrolysis
BAC Bacitracin (10U) resistance
COAG Coagulase
CP Colony pigment
ESC Esculin hydrolysis
FUR Furazolidone (100µg) resistance
MNS Mannose fermentation
M-OX Modified Oxidase
NIT Nitrate reduction to nitrite
NOV Novobiocin (5µg) resistance
ORN Ornithine decarboxylase


Test
Abbreviation Test
PB Polymyxin B (300µg) resistance
PYR Pyrrolidonyl arylamidase (2 hr. broth test)
URE Urease production-Christiansen Urea
Additional tests for Streptococcaceae
Additional tests: Streptococcaceae

Test
Abbreviation Test
ARA Arabinose fermentation
BE Bile esculin
BGUR β-glucuronidase
BS Bile solubility
CAMP CAMP test
CHA Chain morphology predominant from broth
CLU Cluster morphology predominant from broth
CP Colony pigment
GRP Lancefield serogroup
GRPA Lancefield serogroup A
GRPB Lancefield serogroup B
GRPC Lancefield serogroup C
GRPG Lancefield serogroup G
HEM Beta hemolysis
LAP Leucine aminopeptidase
MAN Mannitol fermentation
MGP Methyl-α-D-glucopyranoside
MORPH Gram stain morphology from broth

Additional tests: Streptococcaceae (continued)

Test
Abbreviation Test
NACL Growth in 6.5% NaCl (salt) broth
OPT Optochin sensitivity
PRV Pyruvate utilization
RAF Raffinose fermentation
SAT Satellite test
SOR Sorbitol fermentation
SUC Sucrose fermentation
TINY Colony diameter 0.5 mm at 24 hours
TRD Tetrad morphology predominant from broth
TRE Trehalose fermentation
VA Vancomycin (30μg) resistance
Note: fermentation test results for Streptococcaceae are from 1% sugar

solutions in heart infusion broth.
The following tables show the expected result for each test listed above with each organism in
the database.
• Additional tests for separation of Staph/related genera (Micrococcaceae) (page 46)
• Additional tests for separation of catalase negative gram-positive cocci (page 48)
• Additional tests for separation of non-β hemolytic streptococci and other gram-positive cocci
(page 49)
• Additional tests for separation of β hemolytic streptococci (page 50)
• Additional tests for separation of enterococci (page 51)

Additional tests for separation of Staph/related genera

(Micrococcaceae)
Table 2-E: Additional tests for separation of Staph/related genera (Micrococcaceae)
Organism COAG NOV PB ORN PYR FUR M-OX URE CP ALK NIT MNS ESC VP BAC ARG
K. kristinae / / / / / R + V + / / / + / S -
L. monocytogenes / / / / / / / / / / / + + + / /
Micro/Rel species / / / / - R + V V / / / / / S -
R. dentocariosa - / / / / / / / - / / / / / / /
R. mucilaginosa - / / / / V - / - / / / / / - /
S. aureus + S R - - S - + / + + + - + R /
S. auricularis - S S - + S - - / - V - - - R /
S. capitis - S S - - S - + / - V + - V R /
ssp. capitis
S. capitis - S S - - S - - / - + + - V R /
ssp.urealyticus
S. cohnii - R S - - S - - / - - V - V R /
ssp.cohnii
S. cohnii - R S - V S - + / + - V - V R /
ssp. urealyticus
S. epidermidis - S R - - S - + / V + V - + / /
S. haemolyticus - S S - + S - - / - + - - + / /
S. hominis - S S - - S - + / - V - - V R /
ssp. hominis
S. hominis ssp. - R S - - S - + / - V - - V R /
novobiosepticus
S. hyicus V S R - - S - V / + + + - - / /
S. intermedius + S S - + S - + / + + + - - R /
S. lugdunensis - S R + + S - V / - + + - + / /
S. saprophyticus - R S - - S - + / - - - - + / /
S. schleiferi + S S - + S - + / + + + - + +
/
ssp. coagulans
S. schleiferi - S S - + S - - / + + + - + +
/
ssp. schleiferi
S. sciuri - R S - - S + - / + + V + - R /

Table 2-E: Additional tests for separation of Staph/related genera (Micrococcaceae) (continued)
Organism COAG NOV PB ORN PYR FUR M-OX URE CP ALK NIT MNS ESC VP BAC ARG
S. simulans - S S - + S - + / V + V - V / /
S. warneri - S S - - S - + / - V - - + / /
S. xylosus - R S - + S - + / V V + V V R /
Legend
Value Description
+ Positive
- Negative
V Variable
/ Not tested or not applicable
S Susceptible
R Resistant

Additional tests for separation of catalase negative

gram-positive cocci
Table 2-F: Additional tests for separation of catalase negative gram-positive cocci
Organism PYR LAP ESC BE NACL VA SAT ARG BGUR MORPH
A. defectiva* + + - - - S + - - Chains
A. urinae - + V - + S - - + Clusters (tetrads)
A. viridans + - V V + S - / / Clusters (tetrads)
Enterococcus sp. + + + + + V - V / Chains
Gemella sp. + + - - - S - / / Pairs and Chains
G. adiacens* + + / - - S + - + Chains
G. elegans* + + / - - S + + - Chains
Lactococcus* V + + + V S - / / Chains
Leuconostoc - - V V + R - / / Chains
Pediococcus - + + + V R - / / Clusters
Streptococcus sp. V + V V V S - / / Chains
Vagococcus* + + + + V S - / / Chains
* Organism is not currently in the dried (conventional) gram-positive database.

Additional tests for separation of non-β hemolytic streptococci

and other gram-positive cocci
Table 2-G: Additional tests for separation of non-β hemolytic streptococci and other gram-positive cocci
Organism GRP CAMP BS OPT VP ARG ESC URE SOR MAN HEM
S. agalactiae B + - R / / / / / / Weak beta, gamma
S. bovis grp. D - - + - + - - + Gamma, alpha
S. anginosus grp. A,C,G,F, - - R + + V - - - Beta, alpha, gamma

or Neg
S. mitis/oralis H - - R - V V - V - Alpha
S. mutans / - - R + - V - + + Alpha, rare beta
S. pneumoniae / - + S / / / / / / Alpha
S. salivarius / - - R + - V V - - Gamma, alpha

Additional tests for separation of β hemolytic streptococci

t
Table 2-H: Additional tests for separation of β hemolytic streptococci
Organism Colony
GRP Size VP CAMP PYR TRE SOR Host
S. pyogenes A Large - - + / - Humans
S. agalactiae B Large - + - / - Humans
S. dysgalactiae C, G Large - - - + - Humans

ssp.equismilis
S. dysgalactiae C, L Large - - - + V Animals

ssp.dysgalactiae
S. canis* G Large - + - - - Animals
S. equi C Large - - - - - Animals

ssp. equi
S. equi C Large - - - - + Animals

ssp. zooepidemicus
S. iniae None Large - + + - Fish**
S. porcinus* E, P, V, U, V Large + + + + + Swine**

(may cross react
with commerical B
sera)
Anginosus Group*** A, C, G, F, none Small + - - + - Humans

(<0.5mm at
24 hours)
* Not in current database.

** Human infections associated with handling of non-human host
***See Additional tests for separation of non-β hemolytic streptococci and other gram-positive cocci (page 49) for more
information)

Additional tests for separation of enterococci

Table 2-I: Additional tests for separation of enterococci
Organism M30 CP ARG SOR MAN ARA RAF PRV SUC MGP
E. avium - - - + + + - + + V
E. casseliflavus + + + V + + + V + +
E. durans - - + - - - - - - -
E. faecalis - - + + + - - + + -
E. faecium - - + V + + V - + -
E. gallinarum + - + - + + + - + +
E. hirae - - + - - - + - + -
E. raffinosus - - - + + + + + + V

Tests included on Dried (Conventional) Pos panels
Included tests for Staph/related genera (Micrococcaceae)
Included tests: Staph/related genera (Micrococcaceae)

Test
Abbreviation Test
CV Crystal Violet
MS Micrococcus Screen
NIT Nitrate Reduction
NOV Novobiocin
PGR PNP-β-D-Glucuronide
IDX Indoxyl Phosphatase
VP Voges-Proskauer
OPT Optochin
PHO Phosphatase
BE 40% Bile Esculin
PYR L-Pyrrolidonyl-β-Naphthylamide
ARG Arginine
PGT PNP-β-D-Galactopyranoside
URE Urea
LAC Lactose fermentation

Included tests for Streptococcaceae
Included tests: Streptococcaceae

Test
Abbreviation Test
CV Crystal Violet
MS Micrococcus Screen
NOV Novobiocin
PGR PNP-β-D-Glucuronide
IDX Indoxyl Phosphatase
VP Voges-Proskauer
OPT Optochin
PHO Phosphatase
BE 40% Bile Esculin
PYR L-Pyrrolidonyl-β-Naphthylamide
ARG Arginine
PGT PNP-β-D-Galactopyranoside
URE Urea
LAC Lactose fermentation
NACL Growth in 6.5% Sodium Chloride
RBS Ribose fermentation
INU Inulin fermentation
BAC Bacitracin sensitivity
HEM Beta-Hemolysis

Principles of identification reactions

Test Principle
Crystal Violet (CV) Growth in the presence of low concentrations of crystal violet is used to
distinguish streptococci (positive) from staphylococci (mostly negative).
Micrococcus Screen (MS) Growth in the presence of low concentrations of bacitracin (0.05 μg/mL) is
used to distinguish staphylococci (positive) from micrococci (negative).
Nitrate (NIT) Reduction of nitrate to nitrite is detected by the formation of a red color
following the addition of 0.8% Sulfanilic Acid and 0.5% N, NDimethyl-
alpha-naphthylamine. Streptococci are nitrate negative while most
staphylococci are nitrate positive.
Novobiocin (NOV) Resistance to low concentrations (1.6 μg/mL) of Novobiocin is

characteristic of some staphylococci.
Glycosidases (PGR, PGT) The ability of an organism to produce a specific glycosidase enzyme is
detected by the splitting of the p-nitrophenyl-carbohydrate complex
releasing p-nitrophenol which is yellow in color.
Indoxyl Phosphatase Hydrolysis of indoxyl phosphate by the enzyme indoxyl phosphatase

(IDX) results in an insoluble blue compound. Most coagulase and DNase
positive staphylococci are IDX positive.
Voges-Proskauer (VP) Acetylmethylcarbinol, produced from glucose, reacts with 40% Potassium
Hydroxide and 5% Alpha Naphthol to form a red color.
Optochin (OPT) Susceptibility to optochin is characteristic of Streptococcus pneumoniae.

Other streptococci and staphylococci are not inhibited by optochin.
Phosphatase (PHO) Alkaline phosphatase splits p-nitrophenyl phosphate into inorganic

phosphate and p-nitrophenol which is yellow in color.
Bile Esculin (BE) Organisms capable of growing in 40% bile and hydrolyzing esculin are
detected by the production of a black precipitate resulting from the
reaction of the hydrolytic product esculetin with ferric citrate.
Group D streptococci, some viridans streptococci, and some staphylococci
are BE positive.
Pyrrolidonyl-β- Organisms which produce pyrrolidonase split L-pyrrolidonyl-β-

naphthylamide (PYR) naphthylamide into L-pyrrolidone and β-naphthylamine which combines
with Peptidase reagent (p-dimethyl-aminocinnamaldehyde) to produce a
red color.
Arginine (ARG) Dehydrolization of arginine results in alkalinization of the medium which is

detected by a yellow to red color change in the phenol red indicator.
Urea (URE) The enzyme urease splits urea forming ammonia. The resulting increase
in pH is detected by the phenol red indicator.


Test Principle
Carbohydrates The fermentation of a specific carbohydrate results in acid formation.
(RAF, LAC, TRE, MNS, The consequent pH drop is detected by the phenol red indicator turning
SOR, ARA, RBS, INU, yellow.
MAN)
6.5% NaCl (NACL) Tolerance to 6.5% sodium chloride is demonstrated by growth.

Salt tolerance is used to differentiate enterococci from non-enterococci.
Bacitracin (BAC) Susceptibility to low concentrations of bacitracin is indicated by lack of

growth and is characteristic of Streptococcus pyogenes.
Pyruvate (PRV) Utilization of pyruvate results in acid formation. The resultant drop in pH is
detected by the phenol red indicator turning yellow.
Hemolysis (HEM) Streptolysin S and O which are produced by streptococci cause complete
or partial lysis of the red blood cells in agar containing sheep blood.
More information
See Probability of acceptable identification (page 166) for more information.


3
Dried (Conventional) Neg panels:
• Tests included on Dried (Conventional) Gram-negative panels (page 74)
More information
See References (page 165) for the following large format data tables:
• Dried Neg: Additional tests for the separation of gram-negative glucose fermenters (page 179)
• Dried Neg: Additional tests for the separation of gram-negative glucose nonfermenters/slow glucose
fermenters (page 182)

58 Dried (Conventional) Neg panels: Gram-negative aerobic bacilli
Taxa in database
• Taxa for gram-negative glucose fermenters (page 58)
• Taxa for gram-negative glucose nonfermenters/slow glucose fermenters (page 61)
Taxa for gram-negative glucose fermenters

Table 3-A: Gram-negative glucose fermenters
Aer hydro cplx. Aeromonas hydrophila complex Aeromonas caviae complex
Aeromonas hydrophila
Aeromonas veronii
C. davisae Cedecea davisae Enteric group 15
C. lapagei Cedecea lapagei Enteric group 15
C. neteri Cedecea neteri Enteric group 15
Cedecea sp. 3 Cedecea species 3 N/A
Cedecea sp. 5 Cedecea species 5 N/A
C. amalonaticus Citrobacter amalonaticus Levinea amalonaticus
C. freundii cplx. Citrobacter freundii complex N/A
E. meningo (F) Elizabethkingia meningoseptica Chryseobacterium meningosepticum
E. asburiae Enterobacter asburiae Enteric group 17

Dried (Conventional) Neg panels: Gram-negative aerobic bacilli 59
Table 3-A: Gram-negative glucose fermenters (continued)

E. fergusonii Escherichia fergusonii Enteric group 10
E. hermannii Escherichia hermannii Enteric group 11
E. vulneris Escherichia vulneris Enteric group 1 Alma 1
H. alvei Hafnia alvei Enterobacter hafnia
G. hollisae Grimontia hollisae Vibrio hollisae
K. ascorbata Kluyvera ascorbata Enteric group 8
K. cryocrescens Kluyvera cryocrescens Enteric group 8
L. adecarboxy Leclercia adecarboxylata Enteric group 41
Leminorella sp. Leminorella species Enteric group 57
M. wisconsensis Moellerella wisconsensis Enteric group 46
M. morganii Morganella morganii Proteus morganii
Past-Actin sp. Pasteurella-Actinobacillus species N/A
P. damselae Photobacterium damselae Vibrio damsela
P. shigelloides Plesiomonas shigelloides Aeromonas shigelloides
P. penneri Proteus penneri Proteus vulgaris biogroup 1
P. vulgaris Proteus vulgaris N/A


P. rettgeri Providencia rettgeri Proteus rettgeri biogroups 1-4
P. rustigianii Providencia rustigianii Providencia alcalifaciens biogroup 3
P. stuartii Urea (+) Providencia stuartii Urea (+) Proteus rettgeri biogroup 5
S. Choleraesuis Salmonella enterica serotype Choleraesuis Salmonella choleraesuis
S. paratyphi A Salmonella enterica serotype Paratyphi A Salmonella paratyphi A
S. ent arizonae Salmonella enterica ssp. arizonae Salmonella/Arizona
S. fonticola Serratia fonticola “Serratia” fonticola
S. liquefac cplx. Serratia liquefaciens complex Serratia liquefaciens
S. sonnei Shigella sonnei Shigella group D
Shigella sp. Shigella species Shigella group C, A and B
Shigella boydii N/A
V. alginolyt Vibrio alginolyticus N/A
V. fluvialis Vibrio fluvialis CDC group EF-6, group F Vibrio
V. mimicus Vibrio mimicus Vibrio cholera, Sucrose (-)


V. parahaemolyt Vibrio parahaemolyticus N/A
V. vulnificus Vibrio vulnificus Lactose (+) halophilic Vibrio
Y. entero group Yersinia enterocolitica group Yersinia enterocolitica
Yersenia frederiksenii
Yersinia intermedia
Yersinia kristensenii
Y. ruckeri Yersinia ruckeri Red mouth bacterium
Y. regensburgei Yokenella regensbergei Koserella trabulsii
Taxa for gram-negative glucose nonfermenters/slow glucose

fermenters
Table 3-B: Gram-negative glucose nonfermenters
A. xylosoxidans Achromobacter xylosoxidans ssp. xylosoxidans Achromobacter xylosoxidans
Ac baum/haem Acinetobacter baumannii complex/Acinetobacter Acinetobacter baumannii/

haemolyticus haemolyticus
A. lwoffii Acinetobacter lwoffii Genomospecies 8 and 9
Alcaligenes sp. Alcaligenes species N/A
Alcaligenes faecalis N/A
Alcaligenes odorans N/A
B. bronchiseptica Bordetella bronchiseptica Bordetella bronchicanis
B. cepacia cplx. Burkholderia cepacia complex Burkholderia cepacia
B. pseudomallei Burkholderia pseudomallei Pseudomonas pseudomallei
C. pauculus Cupriavidus pauculus Ralstonia paucula
D. acidovorans Delftia acidovorans Comamonas acidovorans

Table 3-B: Gram-negative glucose nonfermenters (continued)

E. meningo (NF) Elizabethkingia meningoseptica Chryseobacterium meningosepticum
Morax/Psychr sp. Moraxella species/Psychrobacter species N/A
Moraxella atlantae N/A
Moraxella lacunata N/A
O. anthropi Ochrobactrum anthropi Achromobacter sp. VD-1, 2
O. ureolytica Oligella ureolytica CDC group IV E
P. multo SF Pasteurella multocida N/A
Past-Actin sp.SF Pasteurella-Actinobacillus species N/A
Ps fluor/putida Pseudomonas fluorescens/Pseudomonas putida N/A
P. stutzeri Pseudomonas stutzeri CDC group VB
Comamonas testosteroni N/A
R. pickettii Ralstonia pickettii Burkholderia (P.) pickettii
S. putrefaciens Shewanella putrefaciens Pseudomonas putrefaciens

Table 3-B: Gram-negative glucose nonfermenters (continued)

S. multivorum Sphingobacterium multivorum Flavobacterium multivorum
S. spiritivorum Sphingobacterium spiritivorum Flavobacterium spiritivorum
S paucimobilis Sphingomonas paucimobilis Pseudomonas paucimobilis
Vibrio sp. SF Photobacterium damselae Vibrio damsela
Grimontia hollisae Vibrio hollisae
Vibrio species N/A
W. virosa Weeksella virosa CDC group II F
Y. pseudotb. SF Yersinia pseudotuberculosis N/A

information:
species. Below is the list of special characteristics used in conjunction with MicroScan Dried
(Conventional) gram-negative identification panels.
BC Brown Colony S. maltophilia
S. putrefaciens
S. spiritivorum
PO Potato odor S. odorifera 1 and 2
S. multivorum
S. spiritivorum
RPV Red pigment variable S. marcescens
YBC Yellow-brown colony W. virosa
B. zoohelcum

Special characteristics (continued)

YP Yellow pigment E. hermannii
E. vulneris
C. sakazakii
C. indologenes
B. cepacia complex
S. paucimobilis
YWC Yellow wrinkled colony P. stutzeri
P. luteola
P. oryzihabitans
WC Wrinkled colony B. pseudomallei
Footnotes
One or more of the footnotes listed below may be displayed to the right of the biotype number in
the FTN column.
NOTE:
Disregard footnotes C, J, U, V, X, Y, Z, AA, BB, DD, FF, GG, HH, II, JJ, and MM as they are not
applicable for Dried gram-negative panels.
A. To separate Pasteurella multocida from the halophilic Vibrio species, perform a motility test,
and check for growth on MacConkey agar. P. multocida is nonmotile and does not grow on
MacConkey agar.
If motility is positive, retest by emulsifying several colonies into 3 mL of sterile 0.85% saline.
Final turbidity should be equivalent to a 0.5 McFarland Barium Sulfate Standard.
Rehydrate a panel with 25 mL of uninoculated Inoculum Water with PLURONIC® using a

RENOK.
Using a sterile transfer pipette, add one drop (45-50 µL) of the saline suspension to each
identification well including the growth, Cl4, and Cf8 wells. Incubate the panel at 35°C for
16-24 hours.

B. Proteus mirabilis usually has a MIC of <8 µg/mL to ampicillin, Proteus vulgaris and
Morganella morganii usually have MICs of >8 µg/mL to ampicillin.
D. Achromobacter xylosoxidans, Ochrobactrum anthropi and Alcaligenes species may turn the
DCB and all three decarboxylase reactions purple after 42-48 hours incubation.
E. Prior to identifying organisms which appear non-reactive, check to ensure that the organisms
have grown in the phenol red carbohydrates (GLU-MEL).
If there is no growth in the phenol red carbohydrates, one should suspect a fastidious
organism such as a halophilic Vibrio, a Yersinia species which grows better at 25°C, or a
member of the Pasteurella/Actinobacillus group.
A Gram stain should be repeated to confirm it is a gram-negative.
If a halophilic Vibrio is suspected, retest by emulsifying several colonies into 3 mL of sterile

0.85% saline.
Final turbidity should be equivalent to a 0.5 McFarland Barium Sulfate Turbidity Standard.
Rehydrate the panel with 25 mL of uninoculated Inoculum Water with Pluronic using a
RENOK.
identification well including the growth, Cl4 and Cf8 wells. Incubate the panels at 35°C for
16-24 hours.

colorless.
G. Ochrobactrum anthropi does not grow in cetrimide. A. xylosoxidans grows weakly in

cetrimide and usually demonstrates a faint haze throughout the cetrimide well after 42-48
hours incubation.
H. W. virosa may require 42-48 hours to grow in Mueller-Hinton broth. Growth may be very light
after 42-48 hours incubation.

K. This taxon includes one or more of the following species: P. alcaligenes, B. diminuta, P.
pseudoalcaligenes, C. testosteroni and B. vesicularis. These species may be differentiated
as follows:
42 ° OF
Organism Nitrate Arginine Starch SS Centrimide Growth Maltose
P. alcaligenes 54 12 0 38 15 0 0
P. pseudoalcaligenes 90 99 3 84 37 99 0
B. diminuta 3 0 0 1 0 38 0
B. vesicularis 6 0 95 6 0 0 98
C. testosteroni 96 0 0 21 0 0 0
Numbers indicate percent positive reactions.
*PLURONIC® Surfactants. Registered trademark of BASF Corporation, Parsippany, NJ USA
tests listed.
N. Most hemolytic strains of Acinetobacter species, whether glucose positive or glucose

negative belong to Genospecies 4, and are now designated Acinetobacter haemolyticus.
O. This taxon includes one or more of the following species: Aeromonas hydrophila,
Aeromonas caviae complex, Aeromonas veronii biovar sobria, or Aeromonas veronii biovar
veronii.
These species may be differentiated as follows using the results of the tests on the panels:
Organism Arabinose Esculin VP Ornithine Arginine

A. hydrophila 80 90 95 0 100
A. caviae 100 50 0 0 90
A. veronii 10 0 95 100 0
biovar veronii
A. veronii 10 0 90 0 100
biovar sobria

P. Aeromonas hydrophila group will not grow in 6% NaCl and is usually resistant to ampicillin.
Vibrio species will grow in 6% NaCl and are usually susceptible to ampicillin.

• Setup a new conventional/overnight panel at 25°C

Y. intermedia + + +
• Or, setup offline tests at 25°C

Y. intermedia 100 100 80 100
Arizona, California, and Colorado.
If colony morphology, specimen source, and your location in an endemic area suggest a
Yersinia pestis infection it is important to use necessary safety precautions as well as contact
your State Health Department and the physician immediately.

Lecithinase
Organism OF Mannitol (Egg Yolk Agar 25°C)
P. putida 19 0

T. Vibrio species includes the halophilic Vibrios; G. hollisae, P. damselae, V. alginolyticus, V.

fluvialis, V. parahaemolyticus, and V. vulnificus. This group of organisms requires the
addition of salt to biochemical tests for speciation.
If a halophilic Vibrio is suspected, retest by emulsifying several colonies into 3 mL of sterile

0.85% saline. Final turbidity should be equivalent to a 0.5 McFarland Barium Sulfate
Turbidity Standard. Rehydrate the panel with 25 mL of uninoculated Inoculum Water with
PLURONIC using a RENOK.
identification well including the growth, Cl4, and Cf8 wells. Incubate the panels at 35°C for
16-24 hours.
W. Citrobacter freundii complex includes: Citrobacter freundii, Citrobacter braakii, Citrobacter

youngae, Citrobacter werkmanii, Citrobacter sedlakii, Citrobacter rodentium, Citrobacter
gillenii, and Citrobacter murliniae.
Dulcitol
Organism SUC MEL fermentation MLN ORN H2S
C. freundii 89 100 11 11 0 78
C. braakii 20 10 85 5 5 65
C. youngae 7 80 33 0 93 60
C. werkmanii 0 0 0 100 0 100
C. sedlakii 0 100 100 100 100 0
C. rodentium 0 0 0 100 100 0
C. gillenii 33 67 0 100 0 67
C. murliniae 33 33 100 0 0 67

EE. Leminorella sp. includes Leminorella grimontii and Leminorella richardii.

Organism MR CIT
L. richardii 0 0
KK. Ac baumann/haem includes Acinetobacter baumannii complex and Acinetobacter

haemolyticus.

End of footnotes

Additional tests
These tests display the right of the Special Characteristics column when the probability of the
Up to three tests will be printed. The results listed for each test are expressed as positive
percentages. The following table list the additional tests used for the Dried (Conventional)
gram-negative panels.
Note that the additional test tables are different for MicroScan Dried (Conventional) and Rapid
panels.
Additional tests for Gram-negative glucose fermenters
Table 3-C: Additional tests for Gram-negative glucose

fermenters
Test
Abbreviation Test
OXI Oxidase
MR Methyl Red
DNA DNAse (25°C)

Table 3-C: Additional tests for Gram-negative glucose

fermenters (continued)
Test
Abbreviation Test
DUL Dulcitol Fermentation
SAL Salicin Fermentation

Additional tests for Gram-negative glucose nonfermenters/slow glucose

fermenters
Table 3-D: Additional tests for Gram-negative glucose nonfermenters/slow

glucose fermenters
OXI Oxidase
OGL OF Glucose
OLA OF Lactose
OMA OF Maltose
OMT OF Mannitol
OFS OF Sucrose
OXY OF Xylose
DNA DNAse (25°C)
CAT Catalase
42G Growth at 42°C
FLG Flagella: Polar (PO), Peritrichous (PE), None (N)
N2 Gas from Nitrate
3FL >3 Flagella
ST Starch Hydrolysis
TE MIC <4 µg/mL for Tetracycline
LEC Lecithinase (Egg Yolk Agar, 25°C)
MEL Oxidation of Melibiose (check MEL well on MicroScan panel)
Dried Neg: Additional tests for the separation of gram-negative glucose fermenters (page 179)
and Dried Neg: Additional tests for the separation of gram-negative glucose nonfermenters/slow
glucose fermenters (page 182) show the expected result for each test listed with each organism
in the database.

Tests included on Dried (Conventional) Gram-negative

panels
Included tests for glucose fermenters
Table 3-E: Included tests for Gram-negative glucose fermenters

GLU Glucose Fermentation
SOR Sorbitol Fermentation
ARA Arabinose Fermentation
URE Urea Hydrolysis
H2S H2S Production

IND Indole Production
LYS Lysine Decarboxylase
ARG Arginine Decarboxylase
ORN Ornithine Decarboxylase
TDA Tryptophan Deaminase
ESC Esculin Hydrolysis
VP Voges-Proskauer Test
CIT Citrate Utilization
MAL Malonate Utilization
ONPG β-galactosidase
CI4 Resistance to Colistin, MIC > 4 µg/mL
CF8 Resistance to Cephalothin, MIC > 8 µg/mL
OXI Oxidase

Included tests for glucose nonfermenters/slow glucose

fermenters
Table 3-F: Included tests for Gram-negative glucose nonfermenters

URE Urea Hydrolysis
H2S H2S Production
IND Indole Production
ORN Ornithine Decarboxylase
TDA Tryptophan Deaminase
VP Voges-Proskauer Test
MAL Malonate Utilization
ONPG galactosidase
TAR Tartrate Utilization
ACE Acetamide Utilization
CET Cetrimide
OF/G Oxidation of Glucose
P4 Resistance to Penicillin, MIC > 4 µg/mL
K4 Resistance to Kanamycin, MIC > 4 µg/mL
Fd64 Resistance to Nitrofurantoin, MIC > 64 µg/mL
To4 Resistance to Tobramycin MIC > 4 µg/mL
OXI Oxidase


Test Principle
Carbohydrate Fermentation (GLU, The fermentation of a specific carbohydrate results in acid
SUC, SOR, RAF, RHA, ARA, INO, formation and a pH drop is detected by a phenol red indicator.
ADO, MEL)
Urea (URE) The enzyme urease splits urea forming ammonia. The resulting
increase in pH is detected by the phenol red indicator.
Hydrogen Sulfide (H2S) Hydrogen sulfide gas is produced from sodium thiosulfate and
reacts with ferric ions in the medium to produce a black
precipitate.
Indole (IND) The metabolism of tryptophan results in the formation of indole,

which is detected by the addition of Kovac’s reagent. If indole is
present, a red color develops.
Lysine, Arginine, Ornithine (LYS, Decarboxylation of these amino acids results in the formation of
ARG, ORN) basic amines which are detected by the bromcresol purple
indicator.
Tryptophan Deaminase (TDA) Bacteria capable of deaminating tryptophan produce

indolepyruvic acid, which reacts with ferric ammonium citrate in
the medium after the addition of ferric chloride to produce a brown
color.
Esculin Hydrolysis (ESC) Hydrolysis of esculin is detected by ferric ammonium citrate in the
medium, which reacts with the hydrolytic products to form a black
precipitate.
Voges-Proskauer (VP) Acetylmethylcarbinol, produced from glucose, reacts with 40%

Potassium Hydroxide and 5% Alpha Naphthol to form a red color.
Galactosidase (ONPG) β-galactosidase hydrolizes ortho-nitrophenyl-β-D-

galactopyranoside, which liberates the yellow-colored
ortho-nitrophenol.
Citrate, Malonate, Acetamide, The utilization of these substrates as the sole source of carbon for
Tartrate (CIT, MAL, ACE, TAR) metabolism results in a rise in pH that is detected by bromthymol
blue indicator.
Oxidation-Fermentation (OF/G) The oxidation of glucose results in acid formation and a pH drop is
detected by the bromthymol blue indicator. The OF/G is compared
to OF/B (base) to determine if any acid has been produced.


Test Principle
Nitrate (NIT) The ability of an organism to reduce nitrate to nitrite is detected by
the addition of Sulfanilic Acid then N, N-Dimethyl-alpha-
naphthylamine, which produce a red color in the presence of
nitrite.
Cetrimide (CET) Tolerance to cetrimide is demonstrated by growth in Mueller-

Hinton broth supplemented with cetrimide.
Penicillin, Kanamycin, Colistin, Resistance to specific concentrations of these antimicrobial

Cephalothin, Nitrofurantoin, agents is demonstrated by growth.
Tobramycin (P4, K4,CI4, Cf8,
Fd64, To4)
More information
• See Dried Neg: Additional tests for the separation of gram-negative glucose fermenters
(page 179) and Dried Neg: Additional tests for the separation of gram-negative glucose
nonfermenters/slow glucose fermenters (page 182) for a complete data tables that show the
expected result for each test listed with each organism in the database


4
Yeast and yeast-like microorganisms
• Yeast and yeast-like taxa in database (page 80)
• Additional tests for the separation of yeasts (page 89)
• Tests included on Rapid Yeast Identification panels (page 91)

80 Yeast and yeast-like microorganisms
Yeast and yeast-like taxa in database
Table 4-A: Yeast and yeast-like microorganisms

B. capitatus Blastoschizomyces capitatus Trichosporon capitatum
Geotrichum capitatum
C. albicans Candida albicans N/A
C. catenulata Candida catenulata Candida ravautii
C. famata Candida famata Torulopsis candida
Debaryomyces hansenii (perfect state)
C. glabrata Candida glabrata Torulopsis glabrata
C. guilliermondii Candida guilliermondii Pichia guilliermondii

(perfect state)
C. humicola Cryptococcus humicola Candida humicola
C. inconspicua Candida inconspicua Torulopsis inconspicua
C. kefyr Candida kefyr Candida pseudotropicalis
Candida macedoniensis
Kluyveromyces marxianus (perfect state)
C. krusei Candida krusei Issatchenkia orientalis (perfect state)
C. lambica Candida lambica N/A
C. lipolytica Candida lipolytica Saccharomycopysis lipolytica

(perfect state)
C. lusitaniae Candida lusitaniae Clavispora lusitaniae (perfect state)
C. pintolopesii Candida pintolopesii Torulopsis pintolopesii Saccharomyces

telluris (perfect state)
C. parapsilosis Candida parapsilosis group Candida parapsilosis
C. rugosa Candida rugosa N/A
C. stellatoidea Candida stellatoidea N/A
C. tropicalis Candida tropicalis N/A

Yeast and yeast-like microorganisms 81
Table 4-A: Yeast and yeast-like microorganisms (continued)

C. tropicalis(sn) Candida tropicalis Candida paratropicalis (sucrose
negative)
C. viswanathii Candida viswanathii N/A
C. zeylanoides Candida zeylanoides N/A
C. albidus Cryptococcus albidus Cryptococcus albidus

var albidus
Cryptococcus albidus
var diffluens
C. ater Cryptococcus ater N/A
C. gastricus Cryptococcus gastricus N/A
C. laurentii Cryptococcus laurentii N/A
C. melibiosum Cryptococcus melibiosum N/A
C. neo/gatti cplx Cryptococcus neoformans/gatti Cryptococcus neoformans
C. terreus Cryptococcus terreus Cryptococcus himalayensis
C. uniguttulatus Cryptococcus uniguttulatus N/A
Geotrichum sp. Geotrichum species N/A
P. anomala Pichia anomala Hansenula anomala
H. polymorpha Hansenula polymorpha Hansenula angusta
K. lactis Kluyveromyces lactis Kluyveromyces marxianus var. lactis
Candida sphaerica
(imperfect state)
P. farinosa Pichia farinosa N/A
P. wickerhamii Prototheca wickerhamii N/A
Prototheca sp. Prototheca species Prototheca zopfii
Prototheca moriformis
Prototheca stagnora
R. glutinis Rhodotorula glutinis Rhodosporidium sp.

(perfect state)
R. minuta Rhodotorula minuta N/A
R. mucilaginosa Rhodotorula mucilaginosa Rhodotorula rubra

Table 4-A: Yeast and yeast-like microorganisms (continued)

S. cerevisiae Saccharomyces cerevisiae Saccharomyces uvarum
S. salmonicolor Sporobolomyces salmonicolor Sporidiobolus salmonicolor (perfect

state)
T. beigelii cplx Trichosporon beigelii complex Trichosporon beigelii
In addition to probability data, yeast biotype numbers will be accompanied by Microscopic
Morphologic Characteristics. See Microscopic Morphologic Characteristics (in this section) and
Typical characteristics by species (page 83) for more information.
information:
Microscopic Morphologic Characteristics

Microscopic Morphologic Characteristics are unique features which may help to identify a
particular genus or species. Testing for these characteristics (except where indicated) should be
performed on Cornmeal Agar with 1% Tween 80 at 25°C using the Dalmau technique to elicit
typical morphology. (R) indicates a rare characteristic, (V) indicates a variable characteristic.
Table 4-B: Microscopic Morphological characteristics

Abbreviation Morphologic characteristic
AL Achlorophyllous alga. Sporangia and Sporangiospores present
AR Arthroconidia
AS Ascospores present on Yeast Extract-Malt Extract Agar
BA Ballistoconidia
BL Blastoconidia
CA Capsule
CH Chlamydospores
HY True Hyphae
PS Pseudohyphae
YE Yeast cells

Typical characteristics by species

The following table lists typical microscopic morphologic characteristics for the yeast species
included in the database.
Table 4-C: Typical characteristics by species

Species Morphologic characteristic
B. capitatus (T. capitatum) HY, AR, BL
C. albicans BL, PS, CH
C. catenulata BL, PS
C. famata YE
C. glabrata YE
C. guilliermondii BL, PS
C. humicola BL, PS, HY
C. inconspicua YE
C. kefyr BL, PS
C. krusei BL, PS
C. lambica BL, PS
C. lipolytica BL, PS, HY
C. lusitaniae BL, PS, AS (rare)
C. parapsilosis group BL, PS
C. pintolopesii YE, PS (variable)
C. rugosa BL, PS
C. stellatoidea BL, PS, CH
C. tropicalis BL, PS, HY (rare)
C. tropicalis (sn) BL, PS, HY (rare)
C. viswanathii BL, PS
C. zeylanoides BL, PS
C. albidus YE, CA, PS (rare)
C. ater YE,CA
C. gastricus YE, CA
C. laurentii YE, CA, PS (rare)

Table 4-C: Typical characteristics by species (continued)

Species Morphologic characteristic
C. melibiosum YE, CA
C. neo/gatti cplx YE, CA PS (rare)
C. terreus YE, CA PS (rate)
C. uniguttulatus YE, CA
Geotrichum sp. HY, AR
P. anomala PS (variable), AS
H. polymorpha YE, AS
K. lactis PS, AS
P. farinosa PS, AS
P. wickerhamii AL
Prototheca sp. AL
R. glutinis YE, CA (variable), PS (variable)
R. minuta YE, CA (variable)
R. mucilaginosa YE, CA (variable), PS (variable)
S. cerevisiae YE, PS (variable), AS
S. salmonicolor PS (variable), HY (variable), BA
T. beigelii cplx HY, AR, BL, PS

Footnotes
A footnote listed below may be printed to the right of the biotype number in the F column.
The morphological characteristics or additional tests indicated may be used to differentiate the
species listed.
See Additional tests (page 88) for more information about the tests listed in the footnotes.
A. Enzyme pattern of C. lusitaniae may be similar to C. guilliermondii. Perform additional tests:
Organism RA
C. lusitaniae -
C. guilliermondii +
B. Enzyme pattern of C. guilliermondii may be similar to C. lusitaniae and C. parapsilosis grp.

Perform additional tests:
Organism RA CE
C. guilliermondii + +
C. lusitaniae - +
C. parapsilosis grp - -
C. Enzyme pattern of C. famata may be similar to C. guilliermondii and C. lusitaniae.

Perform additional tests and/or check morphological characteristics:
Organism RA PS
C. famata + -
C. guilliermondii + +
C. lusitaniae - +

D. Enzyme pattern of C. rugosa may be similar to C. albicans and C. parapsilosis grp.

Perform additional tests:
Organism GE MA SU FG
C. rugosa - - - -
C. albicans + + + +
C. parapsilosis grp - + + +
E. Enzyme pattern of C. uniguttulatus may be similar to S. salmonicolor. Perform additional

tests and/or check morphological characteristics:
Organism IN PG BA
C. uniguttulatus + - -
S. salmonicolor - + +
F. Enzyme pattern of S. salmonicolor may be similar to Rhodotorula sp. and C.uniguttulatus.

Check morphological characteristics:
Organism BA IN
S. salmonicolor + -
Rhodotorula sp. - -
C. uniguttulatus - +
G. Enzyme pattern of B. capitatus (T. capitatum) may be similar to Geotrichum sp. and
C. krusei. Check morphological characteristics:
Organism HY PS AR BL
B. capitatus + - + +
Geotrichum sp. + - + -
C. krusei - + - +

H. Enzyme pattern of Geotrichum sp. may be similar to B. capitatus (T. capitatum). Check
morphological characteristics:
Organism HY PS AR BL
Geotrichum sp. + - + -
B. capitatus + - + +
I. Enzyme pattern of S. cerevisiae may be similar to C. glabrata. Perform additional tests and/
or check morphological characteristics:
Organism PS MA
S. cerevisiae V +
C. glabrata - -
J. Enzyme pattern of C. glabrata may be similar to S. cerevisiae. Perform additional tests and/
or check morphological characteristics:
Organism PS MA
C. glabrata - -
S. cerevisiae V +
K. Cryptococcus neoformans/gatti complex identification should be confirmed using a phenol

oxidase test (e.g. Bird-seed agar).
End of footnotes

Additional tests
first organism is below 85% (low probability). Up to 3 tests are displayed.
The following table shows the expected result for each test listed with each yeast or yeast-like
organism in the database.
Table 4-D: Additional tests for yeast and yeast-like organisms

Test
Abbreviation Test
CE Cellobiose assimilation
CS Cycloheximide sensitivity, as determined by growth or no growth on

medium containing 0.5% cycloheximide
ER Erythritol assimilation
FG Fermentation of glucose with gas production
FS Fermentation of sucrose with gas production
GE Germ tube formation at 35°C in 2.5-3.0 hours
IN Inositol assimilation
LA Lactose assimilation
KN Nitrate utilization
MA Maltose assimilation
PG Pink to red pigment produced
PO Phenol oxidase enzymes present resulting in pigment production on

caffeic acid or bird seed agar
RA Raffinose assimilation
SU Sucrose assimilation
TR Trehalose assimilation
37 Growth at 37°C on Sabouraud Agar

Additional tests for the separation of yeasts
Table 4-E: Additional tests for the separation of yeasts
Organism Abbr. GE PO IN PG TR FG MA SU ER FS LA RA KN 37 CS 42 CE
B. capitatus - - - - - - - - - - - - - + / - -
C. albicans + - - - + + + + - - - - - + - / -
C. catenulata - - - - - V + - - - - - - V / / -
C. famata - - - - + V + + V V V + - V / / +
C. glabrata - - - - + + - - - - - - - + / / -
C. guilliermondii - - - - + + + + - + - + - + / / +
C. humicola - - V - + - + + + - V V - - / / V
C. inconspicua - - - - - - - - - - - - - + / / -
C. kefyr - - - - V + - + - + + + - + / / V
C. krusei - - - - - + - - - - - - - + / + -
C. lambica - - - - - + - - - - - - - + / / -
C. lipolytica - - - - - - - - + - - - - V / - -
C. lusitaniae - - - - + + + + - V - - - + / / +
C. parapsilosis grp - - - - + + + + - V - - - + / / -
C. pintolopesii - - - - - + - - - - - - - + / / -
C. rugosa - - - - - - - - - - - - - + / / -
C. stellatoidea + - - - + + + - - - - - - + + / -
C. tropicalis - - - - + + + + - + - - - + / / V
C. tropicalis (sn) - - - - + + + V - - - - - + / / V
C. viswanathii - - - - + + + + - V - - - + / / +
C. zeylanoides - - - - + V - - - - - - - - / / V
C. albidus - - + V V - + + - - V + + V / / +
C. ater - - + - + - + + - - + + - - / / +
C. gastricus - - + - + - + - - - + - - - / / +
C. laurentii - - + V + - + + V - + + - V / / +
C. melibiosum - - + - - - - - - - + - - + / / +

Table 4-E: Additional tests for the separation of yeasts (continued)
Organism Abbr. GE PO IN PG TR FG MA SU ER FS LA RA KN 37 CS 42 CE
C. neo/gatti cplx - + + - + - + + V - - + - + / / +
C. terreus - - + - + - V - - - + - + - / / +
C. uniguttulatus - - + - V - + + - - - + - - / / -
Geotrichum sp. - - - - - - - - / - - - - V / / /
P. anomala - - - - + + + + + + - V + V / / +
H. polymorpha - - - - + + + + + - - - + + / / V
K. lactis - - - V + + V + - V + V - V / / +
P. farinosa - - - - V + - - + - V - - + / / V
P. wickerhamii - - - - + - - - - - - - - + / / /
Prototheca sp. - - - - - - - V - - - - - V / / /
R. glutinis - - - + + - + + - - - + + V / / +
R. minuta - - - + + - V V V - V V - V / / V
R. mucilaginosa - - - + + - V + - - - + - V / / V
S. cerevisiae - - - - V + + V - V - V - V / / -
S. salmonicolor - - - + + - V + - - - V + V / / V
T. beigelii cplx - - + - V - V V V - + V - V / / V
Legend
Value Description
+ Positive
- Negative
V Variable
/ Not tested or not applicable

Tests included on Rapid Yeast Identification panels
Table 4-F: Tests included on Rapid Yeast Identification panels

Abbreviation Substrates
HPR Hydroxyproline β-naphthylamide
ILE L-Isoleucine β-naphthylamide
PRO L-Proline β-naphthylamide
TYR L-Tyrosine β-naphthylamide
GLY Glycine-β-naphthylamide
GGLY Glycylglycine-β-naphthylamide
GLAR Glycyl-L-arginine 4-methoxy-β-naphthylamide
GLPR Glycyl-L-proline 4-methoxy-β-naphthylamide
AARG L-Arginyl-L-arginine-β-naphthylamide
LYAL L-Lysyl-L-alanine 4-methoxy-β-naphthylamide
ALA L-alanine 4-methoxy-β-naphthylamide
STY L-seryl-L-tyrosine β-naphthylamide
URE Urea
IDX 3-Indoxyl phosphate
HIS L-Histidine β-naphthylamide
SUC1, SUC2* Sucrose
TRE Trehalose
AGL1, AGL2* p-nitrophenyl-α-D-glucopyranoside
BGL p-nitrophenyl-β-D-glucopyranoside
BGAL o-nitrophenyl-β-D-galactopyranoside
BDF p-nitrophenyl-β-D-fucopyranoside
AGAL p-nitrophenyl-α-D-galactopyranoside
NAG p-nitrophenyl-N-acetyl-β-D-glucosamine
CELL p-nitrophenyl-β-D-cellobiose
NGAL p-nitrophenyl-N-acetyl-β-D-galactosaminide
* Different formulations of the same substrate are used.

More information

5
Anaerobes
• Anaerobe taxa in database (page 94)
• Additional identification tests for Rapid Anaerobe Identification panels (page 98)
• Tests included on Rapid Anaerobe Identification panels (page 101)
More information
• Anaerobes: Additional identification tests for anaerobic nonspore-forming gram-negative bacilli

(page 185)

94 Anaerobes
Anaerobe taxa in database

Anaerobic bacteria are characterized by Gram stain, morphology and sporeforming capabilities.
The four categories are:
• Anaerobic cocci (in this section)
• Clostridia (in this section)
• Anaerobic nonspore-forming gram-negative bacilli (NSF-GNB) (page 95)
• Anaerobic nonspore-forming gram-positive bacilli (NSF-GNB) (page 96)
Anaerobic cocci
Table 5-A: Anaerobic cocci
Synonym/Other
A. fermentans Acidaminococcus fermentans N/A
A. prevotii Anaerococcus prevotii Peptostreptococcus prevotii
A. tetradius Anaerococcus tetradius Peptostreptococcus tetradius
F. magna Finegoldia magna Peptostreptococcus magnus
P. anaerob/stomat Peptostreptococcus anaerobius/stomatus Peptostreptococcus anaerobius
P. asaccharolyt Peptoniphilus asaccharolyticus Peptococcus asaccharolyticus
S. sacchrolyt Staphylococcus saccharolyticus Peptococcus saccharolyticus
V. parvula Veillonella parvula N/A
Clostridia
Table 5-B: Clostridia
C. baratii Clostridium baratii Clostridium barati
C. bifermentans Clostridium bifermentans N/A
C. butyricum Clostridium butyricum N/A
C. cadaveris Clostridium cadaveris Clostridium capitovale
C. clostridiofrm Clostridium clostridioforme Bacteroides clostridiiformis
C. difficile Clostridium difficile N/A
C. histolyticum Clostridium histolyticum N/A

Anaerobes 95
Table 5-B: Clostridia (continued)

C. innocuum Clostridium innocuum N/A
C. perfringens Clostridium perfringens N/A
C. ramosum Clostridium ramosum N/A
C. septicum Clostridium septicum N/A
C. sordellii Clostridium sordellii N/A
C. sporogenes Clostridium sporogenes N/A
C. subterminale Clostridium subterminale N/A
C. tertium Clostridium tertium N/A
C. tetani Clostridium tetani N/A
Anaerobic nonspore-forming gram-negative bacilli (NSF-GNB)

Table 5-C: Anaerobic nonspore-forming gram-negative bacilli (NSF-GNB)
B. eggerthii Bacteroides eggerthii N/A
B. fragilis Bacteroides fragilis Bacteroides fragilis ssp. fragilis
B. ovatus Bacteroides ovatus Bacteroides fragilis ssp. ovatus
B. thetaiota Bacteroides thetaiotaomicron Bacteroides fragilis ssp. thetaiotaomicron
B. uniformis Bacteroides uniformis N/A
B. ureolyticus Bacteroides ureolyticus Bacteroides corrodens
B. vulgatus Bacteroides vulgatus Bacteroides fragilis ssp. vulgatus
Capnocytopha sp. Capnocytophaga species DF-1; Bacteroides ochraceus
F. mortiferum Fusobacterium mortiferum N/A
F. necrophorum Fusobacterium necrophorum Sphaerophorous necrophorous
F. nucleatum Fusobacterium nucleatum Fusobacterium fusiforme
F. varium Fusobacterium varium N/A
P. distasonis Parabacteroides distasonis Bacteroides distasonis
P. bivia Prevotella bivia Bacteroides bivius
P. buccae Prevotella buccae Bacteroides buccae

96 Anaerobes
Table 5-C: Anaerobic nonspore-forming gram-negative bacilli (NSF-GNB) (continued)

P. corporis Prevotella corporis Bacteroides corporis
P. disiens Prevotella disiens Bacteroides disiens
P. melaninogen Prevotella melaninogenica Bacteroides melaninogenicus
P. oralis grp. Prevotella oralis group Bacteroides oralis
P. asaccharolyt Porphyromonas asaccharolytica Bacteroides asaccharolyticus
P. gingivalis Porphyromonas gingivalis Bacteroides gingivalis
Anaerobic nonspore-forming gram-positive bacilli (NSF-GNB)

Table 5-D: Anaerobic nonspore-forming gram-positive bacilli (NSF-GNB)
Synonym/Other
A. israelii Actinomyces israelii N/A
A. odontolytic Actinomyces odontolyticus N/A
A. viscosus Actinomyces viscosus N/A
B. dentium Bifidobacterium dentium N/A
E. lentum Eggerthella lentum Eubacterium lentum
E. limosum Eubacterium limosum N/A
Lactobacillus sp. Lactobacillus species N/A
P. acnes Propionibacterium acnes N/A
P. granulosum Propionibacterium granulosum N/A
information:
• Additional identification tests for Rapid Anaerobe Identification panels (page 98)

Anaerobes 97
Table 5-E: Special characteristics

Abbreviation Special characteristic Organism(s)
RFBP Red fluorescence or black pigmentation P. asaccharolytica
P. melaninogenica, P. corporis
P. gingivalis
PC Pitting colonies B. ureolyticus
FEC Fried egg colony F. mortiferum
CF Colonies fluoresce a chartreuse color F. nucleatum, C. difficile

under long wave UV light
GC Greening around colony F. nucleatum
MTC Molar tooth colony A. israelii
AG Aerobic growth C. histolyticum, C. tertium
PP Pink pigment A. odontolyticus
FC Forked cells B. dentium
LB Large bacillus C. bifermentans
OCGN Occasionally gram-negative C. butyricum, C. ramosum
GNS Usually gram-negative, spindle-shaped C. clostridiforme
DZH Double zone of hemolysis C. perfringens
UP Urease positive B. ureolyticus, C. sordellii
SC Swarming colony C. septicum, C. sporogenes
GLC Confirm identification by gas liquid F. magna, A. prevotii

chromatography (GLC)
LP Lipase positive F. necrophorum
LN Lipase negative F. nucleatum

98 Anaerobes
Additional identification tests for Rapid Anaerobe

Identification panels
Clostridia
Table 5-F: Additional identification tests for clostridia
Organism LEC LIP AER SPR IND
C. baratii + - - RS -
C. bifermentans + - - OS +
C. butyricum - - - OS -
C. cadaveris - - - OT +
C. clostridioforme - - - OS V
C. difficile - - - OS -
C. histolyticum - - V OS -
C. innocuum - - - OT -
C. perfringens + - - OS -
C. ramosum - - - OT -
C. septicum - - - OS -
C. sordellii + - - OS +
C. sporogenes - + - OS -
C. subterminale V - - OS -
C. tertium - - + OT -
C. tetani - - - RT V
Legend
LEC Lecithinase V Variable
LIP Lipase O Oval
AER Aerotolerance R Round
SPR Spore location/shape S Subterminal
IND Indole T Terminal
+ Positive V Variable
- Negative

Anaerobes 99
Anaerobic nonspore-forming gram-negative bacilli

See the large format data table Anaerobes: Additional identification tests for anaerobic
nonspore-forming gram-negative bacilli (page 185) for more information.
Anaerobic cocci
Table 5-G: Additional identification tests for anaerobic cocci
Organism GS SPS CAT GLU MAL SUC IND NIT

A. fermentans - - - -
P. anaerob/stomat + S - W + W- -
P. asaccharolyt + R - - - - +
F. magna + R - - - - -
A. prevotii + R - W W W- -
A. tetradius + R - + + + -
S. sacchrolyt + +
V. parvula - V - +
Legend
GS Gram Stain reaction + Positive
SPS Sodium polyanethol sulfonate - Negative
CAT Catalase W Weak
GLU Glucose fermentation V Variable
MAL Maltose fermentation R Resistant
SUC Sucrose fermentation S Susceptible
IND Indole
NIT Nitrate reduction

100 Anaerobes
Anaerobic nonspore-forming gram-positive bacilli

Table 5-H: Additional identification tests for anaerobic nonspore-forming
gram-positive bacilli
Organism CAT CO2 NIT ESC IND PIG

A. israelii - + + + - -
A. odontolytic - + + + - Pink/Red
A. viscosus + + + + - -
B. dentium V - - + - -
E. lentum V - + - - -
E. limosum - - V V - -
Lactobacillus sp. - V V V - -
P. acnes + V + - + -
P. granulosum + V - - - -
Legend
CAT Catalase + Positive
CO2 Growth in CO2 - Negative
NIT Nitrate reduction V Variable
IND Indole
PIG Pigment

Anaerobes 101
Tests included on Rapid Anaerobe Identification

panels
Table 5-I: Additional tests

Abbreviation Substrates
BGAL p-nitrophenyl--D-galactopyranoside
AGAL p-nitrophenyl--D-galactopyranoside
BPO4 bis-p-nitrophenyl-phosphate
NGLU p-nitrophenyl-n-acetyl--D-glucosaminide
AGL p-nitrophenyl--D-glucopyranoside
BGL o-nitrophenyl--D-glucopyranoside
PO4 p-nitrophenyl-phosphate
AFU p-nitrophenyl--L-fucopyranoside
MNP p-nitrophenyl--D-mannopyranoside
LEU L-Leucine--naphthylamide
MET DL-Methionine--naphthylamide
LYB L-Lysine--naphthylamide (alkaline)
LYA L-Lysine--naphthylamide (acid)
GGLY Glycylglycine--naphthylamide
GLY Glycine--naphthylamide
PRO L-Proline--naphthylamide
ARG L-Arginine--naphthylamide
PYR L-Pyrrolidonyl--naphthylamide
TRY L-Tryptophan--naphthylamide
TRE Trehalose
URE Urea
IND Indole
NIT Nitrate

102 Anaerobes
More information
See Anaerobes: Additional identification tests for anaerobic nonspore-forming gram-negative
bacilli (page 185) for a complete data tables that show the expected result for each test listed
with each organism in the database

6
Haemophilus - Neisseria
• Haemophilus - Neisseria taxa in database (page 104)
• Tests included on HNID panel (page 108)
• HNID Additional tests for the separation of Haemophilus and Neisseria (page 109)

104 Haemophilus - Neisseria
Haemophilus - Neisseria taxa in database
Table 6-A: Haemophilus - Neisseria

A. aphrophilus Aggregatibacter aphrophilus Haemophilus aphrophilus
G. vaginalis Gardnerella vaginalis N/A
H. haemolyticus Haemophilus hemolyticus N/A
H. influenzae I Haemophilus influenzae I N/A
H. influenzae II Haemophilus influenzae II N/A
H. influenzae III Haemophilus influenzae III N/A
H. influenzae IV Haemophilus influenzae IV N/A
H. influenzae V Haemophilus influenzae V N/A
H. influenzae VI Haemophilus influenzae VI Haemophilus influenzae,

biotype V, Oberhofer classification
H. influenzae VII Haemophilus influenzae VII Haemophilus influenzae,

biotype VI, Killian classification
H. parainfluenzae I Haemophilus parainfluenzae I N/A
H. parainfluenzae II Haemophilus parainfluenzae II N/A
H. parainfluenzae III Haemophilus parainfluenzae III N/A
H. parainfluenzae IV Haemophilus parainfluenzae IV N/A
M. catarrhalis Moraxella catarrhalis Branhamella catarrhalis
N. gonorrhoeae Neisseria gonorrhoeae N/A
N. lactamica Neisseria lactamica N/A
N. meningitidis Neisseria meningitidis N/A
N. mucosa (NIT+) Neisseria mucosa (NIT+) N/A
Neisseria sp. Neisseria species N/A

Haemophilus - Neisseria 105
information:
• Footnotes (in this section) (FTN)
Footnotes
In addition to probability data, biotype numbers may be accompanied by supplemental
information in the form of footnotes.
A. Neisseria gonorrhoeae will grow on selective media and should not grow at 22°C on
Chocolate or Blood Agar. Neisseria cinerea, a very nonreactive Neisseria, may grow on
selective media. For nonreactive Neisseria, additional testing may be required for
confirmation of identification.
Organism MTM RT NA SO NO3

N. gonorrhoeae + - - + -
N. cinerea V - + - -
N. elongata - + + - -
N. flavescens - + + - -
Kingella denitrificans + NT + - +
B. Neisseria meningitidis is usually Maltose positive, however, strains have been isolated that
are Glucose and/or Maltose negative. Strains that are both Maltose and NGL negative may
be mis-identified as Neisseria gonorrhoeae. Additional testing may be required to confirm the
identification of these unusual strains.
Organism MTM RT NA PSS SO

N. meningitidis + - V - -
N. gonorrhoeae + - - - +
N. polysaccharea V - + + -

Organism MTM RT NA PSS SO

N. subflava bv. subflava - + + - -
N. subflava bv. flava - + + - -
N. subflava bv. perflava V + + + -
C. Moraxella catarrhalis does not produce acid from any sugars. For other Neisseria with similar
characteristics expected results are listed in the following table:
Organism MTM RT NA DNA PSS

N. cinerea V - + - -
N. elongata - + + - -
N. flavescens - + + - +
M. catarrhalis V + + + -
D. Confirmation of Neisseria gonorrhoeae by an alternate method is recommended for

specimens with legal and/or medical implications.
E. Aggregatibacter aphrophilus includes V factor dependent and V factor independent isolates.
End of footnotes

Additional tests
first organism is below 85% (low probability). Up to three tests will be printed. The results for
each test are expressed as positive (+), negative (-) or variable (v) reactions.
The following table lists the additional tests used for Haemophilus and Neisseria on HNID
panels.
Table 6-B: Additional tests

Test
Abbreviation Test
MTM Growth on Modified Thayer Martin
RT Growth on Chocolate or Blood Agar at 22°C
NA Growth on Nutrient Agar
DNA DNase
SO Super Oxol (30% Hydrogen Peroxide)
PSS Polysaccharide from Sucrose
XF X Factor
VF V Factor
CAT Catalase
XYL Xylose
MAN Mannose
HNID Additional tests for the separation of Haemophilus and Neisseria (page 109) shows the
expected result for each test listed above with each organisms in the database.

Tests included on HNID panel
Table 6-C: Tests included on HNID panel

Substrate Abbreviation
IDX Indoxyl Phosphate
NO3 Nitrate
NO2 Nitrite
GLU Glucose
SUC Sucrose
MAL Maltose
FRU Fructose
LAC Lactose
GAL o-nitrophenyl--D-galactoside
URE Urea
ORN Ornithine
IND Indole
PRO L-Prolyl--Naphthylamide
NGL N--L-Glutamyl--Naphthylamide
ZAR N--Benzoyl-DL-Arginine--Naphthylamide
AGL p-nitrophenyl--D-glucoside
STA Starch
BL Beta-Lactamase
More information

HNID Additional tests for the separation of Haemophilus and

Neisseria
Table 6-D: HNID Additional tests for the separation of Haemophilus and Neisseria
Organism MTM RT NA DNA SO PSS XF VF CAT XYL MAN
A. aphrophilus / / / / / / - v - - +
H. influenzae / / / / / / + + + + -
H. haemolyticus / / / / / / + + + v -
H. parainfluenzae / / / / / / - + v - +
M. catarrhalis v + + + - - / / / / /
N. gonorrhoeae + - - - + - / / / / /
N. meningitidis + - v - - - / / / / /
N. lactamica + v + - - - / / / / /
N. subflava v + + - - v / / / / /
N. sicca - + + - - + / / / / /
N. mucosa - + + - - + / / / / /
N. flavescens - + + - - + / / / / /
/ = Not Applicable


7
Vancomycin and Streptomycin Synergy
Screen Susceptibility algorithm
This section includes the following topic:
• Dried (Conventional) gram-positive panel (page 112)

112 Vancomycin and Streptomycin Synergy Screen Susceptibility algorithm
Dried (Conventional) gram-positive panel

The WalkAway System software offers you a choice to hold Enterococcus isolates for 24 hours.
Susceptibility algorithm
The algorithm used by the WalkAway Systems software to determine Vancomycin and
Streptomycin Synergy Screen susceptibility for Enterococcus isolates is:
1. Read all MICs and biochemicals on Pos MIC and Pos Combo panels at 16 or 18 hours.
2. Store all MIC results.
If Then
Vancomycin >16 Report results as final (resistant)
AND
Streptomycin Synergy Screen >1000
Vancomycin ≤16 Read and report Vancomycin at 24 hours.
Streptomycin Synergy Screen ≤1000 Read and report Streptomycin Synergy Screen at 24
hours.
NOTE:
For gram-positive combo panels, the panel will be processed as an Enterococcus
isolate when the family = Streptococcaceae AND beta-hemolysis = negative.
Gram-positive MIC panels will be processed as an Enterococcus only when the following
organisms are selected:
• E. avium • E. raffinosus
• E. casseliflavus • E. mundtii
• E. durans • Enterococcus sp.
• E. durans/hirae • Gram-Pos Cocci
• E. faecalis • Gram-Pos Other 1
• E. faecium • Gram-Pos Other 2
• E. faecium grp. • Gamma Hemo Streptococcus
• E. gallinarum • Group D Streptococcus
• E. hirae • Streptococcus sp.

8
Panel Processing (Read Times)
• Rapid Gram-negative ID 3 panels (page 114)
• Dried (Conventional) Gram-positive panels (page 115)
• Dried (Conventional) Gram-negative panels (page 115)
• Rapid Yeast ID panels (page 117)
• HNID panels (page 117)
• Rapid Neg ID 4 panels (page 117)
• Rapid Pos ID 2 panels (page 118)
• MICroSTREP plus panels (page 118)

114 Panel Processing (Read Times)
Overview
This section provides the read times for all MicroScan panels.
About relative read times

• “at” read times are relative to when the panel was placed into the WalkAway system.
• “after” read times are relative to the previous operation on the same panel.
About overtime
The term overtime refers to the amount of time you can delay an operation before the panel is
aborted by the WalkAway system (with the exception “Aborted - Overtime”).
Rapid Gram-negative ID 3 panels
Table 8-A: Rapid Gram-negative ID 3 panels processing and read times

Operation Read time Overtime of
Panel barcode scan 1 second N/A
Initial ID read at 40 minutes 25 minutes
Final ID read* at 2 hours, 20 minutes 1 hour
*Total processing time between barcode scan and the panel read.
Notes:
If a Rapid Neg ID 4 panel exceeds the overtime at the 40 minute read or 2 hour 20 minute
read, the System aborts the panel.

Panel Processing (Read Times) 115
Dried (Conventional) Gram-positive panels
Table 8-B: Dried (Conventional) Gram-positive panels

processing and read times
Oil dispense after ASAP initial read
Initial read at 2 hours, 45 minutes 1 hour
Second read at 16 or 18 hours* 24 hours*
Third read at 21 hours 24 hours*
If needed:
Oxacillin read at 24 hours 2 hours
Vancomycin read at 24 hours 2 hours
Streptomycin at 24 hours 2 hours

Synergy
Screen read
Fourth read at 42 hours 6 hours
When ready (after 2nd, 3rd, or 4th read):
Dispense ASAP 30 minutes
Reagent reread after 20 minutes 30 minutes
* See Notes for Dried (Conventional) Gram-positive and Gram-negative

panels (page 116)
Dried (Conventional) Gram-negative panels
Table 8-C: Dried (Conventional) Gram-negative panels

processing and read times
Second read at 16 or 18 hours* 24 hours*
Third read at 21 hours 24 hours*

Table 8-C: Dried (Conventional) Gram-negative panels

processing and read times (continued)
Fourth read at 42 hours 6 hours
When ready (after 2nd, 3rd, or 4th read):
Reagent reread after 20 minutes 30 minutes
* See Notes for Dried (Conventional) Gram-positive and Gram-negative

panels (page 116)
Notes for Dried (Conventional) Gram-positive and

Gram-negative panels
• The second read for Gram-positive and Gram-negative dried (conventional) panels can be
customized for 16 or 18 hours. You can select this feature in the Customize System program.
Each laboratory should evaluate their workflow to determine the optimum second read time.
• For all Dried (Conventional) Gram-positive and Gram-negative panels: The panel reading
criteria as specified in the appropriate package insert are evaluated after the 2nd, 3rd, and
4th reads. If the panel meets the criteria, reagents are dispensed and reactions reread after
the appropriate interval. If the criteria are not met, the panel is rescheduled for a later read.
• The second and third readings are given variable overtime allowances. The correct overtime
is determined for each panel such that the latest time that the second or third read could
occur is 24 hours. If the second read occurs after more than 21 hours, the third read is
skipped. If the second read occurs between 20 and 21 hours, then the third read follows the
second read by one hour. In all cases, the MIC’s are evaluated and stored after the second
read.
• Staph organisms which are determined to be susceptible to Oxacillin at the second read are
reread for Oxacillin at 24 hours.
• Enterococci organisms (or β-hemolytic negative streptococci) which are determined to be

susceptible to Vancomycin and/or Streptomycin Synergy Screen at the second read are
reread for Vancomycin and/or Streptomycin Synergy Screen at 24 hours.
The 24 hour read occurs completely independent of the 21 and 42 hour biochemical
reads—it is possible for a Pos Combo to be read at 2 (or 1), 16 (or 18), 21, 24, and 42 hours.
• MIC-only panels are never read at 21 or 42 hours and never add reagents.
• ID-only panels are never read at 24 hours, otherwise, they are processed the same as
combo panels.

Panel Processing (Read Times) 117
Rapid Yeast ID panels
Table 8-D: Rapid Yeast ID panels processing and read times

Initial read at 30 minutes 20 minutes
Second read at 4 hours 30 minutes
Reread after 7 minutes 8 minutes
HNID panels
Table 8-E: HNID panels processing and read times

Initial read at 20 minutes 20 minutes
Second read at 4 hours 30 minutes
Reread after 5 minutes 10 minutes
Rapid Neg ID 4 panels
Table 8-F: Rapid Neg ID 4 panels processing and read times

Final ID read* at 2 hours, 20 minutes 1 hour
Notes:
If a Rapid Neg ID 4 panel exceeds the overtime at the 40 minute read or 2 hour 20
minute read, the System aborts the panel.

Rapid Pos ID 2 panels
Table 8-G: Rapid Pos ID 2 panels processing and read times

Final ID read* at 2 hours 40 minutes
Notes:
If a Rapid Pos ID 2 panel exceeds the overtime at the 40 minute read or 2 hour 20
minute read, the System aborts the panel.
MICroSTREP plus panels

This section describes MICroSTREP plus panels (for streptococcal isolate testing on panel types
supported by the WalkAway system).
Table 8-H: MICroSTREP plus panels processing and read times

Second read at 20 hours 2 hours

9
Rapid Neg ID 4 panels:
• Gram-negative aerobic bacilli taxa in database (page 120)
• Tests included on Rapid Neg ID 4 Neg panels (page 141)
More information
Rapid Neg ID 4: Additional tests for the separation of gram-negative aerobic bacilli (page 187)

Gram-negative aerobic bacilli taxa in database

• Taxa for gram-negative fermenters (in this section)
• Taxa for gram-negative nonfermenters (page 128)
Taxa for gram-negative fermenters

Table 9-A: Gram-negative fermenters - Gram-negative aerobic bacilli
A. caviae cplx Aeromonas caviae complex Aeromonas caviae
Aer hyd/trot/ver Aeromonas hydrophila N/A
Aeromonas trota N/A
A. jandaei Aeromonas jandaei N/A
A. schubertii Aeromonas schubertii N/A
Aeromonas sp. Aeromonas species N/A
Aeromonas caviae N/A
Aeromonas hydrophila N/A
Aeromonas jandaei N/A
Aeromonas schubertii N/A
Aeromonas trota N/A
A. actinomycetem Aggregatibacter actinomycetemcomitans Actinobacillus actinomycetemcomitans
Cedecea davisae Cedecea davisae N/A
Cedecea lapagei Cedecea lapagei N/A
Cedecea neteri Cedecea neteri N/A
Cedecea sp. 3/5 Cedecea species 3 N/A
Cedecea sp. Cedecea species N/A

Table 9-A: Gram-negative fermenters - Gram-negative aerobic bacilli (continued)

Cedecea davisae N/A
Cedecea lapagei N/A
Cedecea neteri N/A
C. amalonaticus Citrobacter amalonaticus N/A
C. farmeri Citrobacter farmeri Citrobacter amalonaticus bg 1
C. freundii cplx Citrobacter freundii complex N/A
Citrobacter freundii N/A
Citrobacter gillenii N/A
Citrobacter murliniae N/A
Citrobacter rodentium N/A


Citrobacter sp. Citrobacter species N/A
Citrobacter amalonaticus Citrobacter amalonaticus bg 1
Citrobacter farmeri Citrobacter diversus
Citrobacter koseri N/A
Citrobacter freundii N/A
Citrobacter gillenii N/A
Citrobacter murliniae N/A
Citrobacter rodentium N/A
E. asburiae Enterobacter asburiae N/A
E. hormaechei Enterobacter hormaechei N/A


Enterobacter sp. Cronobacter sakazakii Enterobacter sakazakii
Enterobacter species N/A
Enterobacter aerogenes N/A
Enterobacter agglomerans N/A
Enterobacter asburiae N/A
Enterobacter cancerogenus Enterobacter taylorae
Enterobacter cloacae N/A
Enterobacter gergoviae N/A
Enterobacter hormaechei N/A
Kluyvera intermedia Enterobacter intermedius
E. albertii Escherichia albertii N/A
E. coli O157:H7 Escherichia coli O157:H7 N/A
E. fergusonii Escherichia fergusonii N/A
E. hermannii Escherichia hermannii N/A
E. vulneris Escherichia vulneris N/A
Escherichia sp. Escherichia species N/A
Escherichia albertii N/A
Escherichia fergusonii N/A
Escherichia hermannii N/A
Escherichia vulneris N/A
G. hollisae Grimontia hollisae N/A
Hafnia alvei Hafnia alvei N/A


Klebsiella sp. Klebsiella species N/A
Klebsiella oxytoca N/A
Klebsiella ozaenae N/A
Klebsiella pneumoniae N/A
Klebsiella rhinoscleromatis N/A
Kluyvera sp. Kluyvera species N/A
Kluyvera ascorbata N/A
Kluyvera cryocrescens N/A
L. adecarboxy Leclercia adecarboxylata N/A
Leminorella sp. Leminorella species N/A
M. wisconsensis Moellerella wisconsensis N/A
M. morganii Morganella morganii N/A
Ppneu/Aure/Mhaem Actinobacillus ureae Pasteurella ureae
Pasteurella sp. Pasteurella species N/A
Pasteurella aerogenes N/A
Pasteurella multocida N/A
P. damsela Photobacterium damsela N/A
P. luminescens Photorhabdus luminescens N/A
P. shigelloides Plesiomonas shigelloides N/A


Prt vulg/penneri Proteus penneri N/A
Proteus sp. Proteus species N/A
Proteus mirabilis N/A
Proteus penneri N/A
P. rettgeri Providencia rettgeri N/A
P. rustigianii Providencia rustigianii N/A
Providencia sp. Providencia species N/A
Providencia alcalifaciens N/A
Providencia rettgeri N/A
Providencia rustigianii N/A
Providencia stuartii N/A
S. Paratyphi A Salmonella enterica serotype Paratyphi A Salmonella paratyphi A
Salmonella enterica serotype Choleraesuis N/A
Salmonella enterica ssp. arizonae Salmonella/Arizona
S. ficaria Serratia ficaria N/A
S. fonticola Serratia fonticola N/A
S. liquefac cplx Serratia liquefaciens complex Serratia liquefaciens


Serratia sp. Serratia species N/A
Serratia ficaria N/A
Serratia fonticola N/A
Serratia liquefaciens complex Serratia liquefaciens
Serratia marcescens N/A
Serratia plymuthica N/A
Serratia rubidaea N/A
Shigella sp. Shigella species N/A
Shigella boydii N/A
Shigella sonnei N/A
V. para/algino Vibrio parahaemolyticus N/A
Vib fluv/furn Vibrio fluvialis N/A
V. metschnikovi Vibrio metschnikovii N/A
V. mimicus Vibrio mimicus N/A
V. vulnificus Vibrio vulnificus N/A


Vibrio sp. Grimontia hollisae Vibrio hollisae
Photobacterium damselae Vibrio damsela
Vibrio species N/A
Vibrio metschnikovii N/A
Vibrio mimicus N/A
Y. entero group Yersinia enterocolitica group N/A
Yersinia enterocolitica N/A
Yersinia frederiksenii N/A
Yersinia intermedia N/A
Yersinia kristensenii N/A
Y. ruckeri Yersinia ruckeri N/A
Yersinia sp. Yersinia species N/A
Yersinia enterocolitica group N/A
Yersinia pseudotuberculosis N/A
Yersinia ruckeri N/A
Y. regensburgei Yokenella regensburgei Koserella trabulsii

Taxa for gram-negative nonfermenters

Table 9-B: Gram-negative nonfermenters - gram-negative aerobic bacilli
Ac baum/haem Acinetobacter baumannii complex Acinetobacter baumannii/haemolyticus
A. lwoffii Acinetobacter lwoffii N/A
Acinetobacter sp. Acinetobacter species N/A
Acinetobacter baumannii complex N/A
Acinetobacter lwoffii N/A
Alsp/Ac. xyl/Cpau Alcaligenes species N/A
Alcaligenes faecalis Alcaligenes odorans
Achromobacter denitrificans Achromobacter xylosoxidans ssp.

denitrificans
Achromobacter xylosoxidans Achromobacter xylosoxidans ssp.

xylosoxidans
Cupriavidus pauculus Ralstonia paucula
B. bronchisept Bordetella bronchiseptica N/A
B. cepacia cplx Burkholderia cepacia complex Burkholderia cepacia
B. gladioli Burkholderia gladioli N/A
B. pseudomallei Burkholderia pseudomallei N/A
B. diminuta Brevundimonas diminuta Pseudomonas diminuta
B. vesicularis Brevundimonas vesicularis Pseudomonas vesicularis
Brevundimonas sp. Brevundimonas species N/A

Table 9-B: Gram-negative nonfermenters - gram-negative aerobic bacilli (continued)

Chryseobact sp. Chryseobacterium species N/A
Chryseobacterium indologenes N/A
Elizabethkingia meningoseptica Chryseobacterium meningosepticum
D. acido/C. test Delftia acidovorans Comamonas acidovorans
Comamonas testosteroni Pseudomonas testosteroni
E. menigosepti Elizabethkingia meningoseptica Chryseobacterium meningosepticum
E. corrodens Eikenella corrodens N/A
Kingella sp. Kingella species N/A
Kingella kingae N/A
Kingella denitrificans N/A
Morax/Psychr spp. Moraxella species N/A
Psychrobacter species N/A
N. elon/weav/zoo Neisseria elongata Neisseria elongata/EF-4B/
Neisseria weaveri Neisseria weaveri
Neisseria zoodegmatis N/A
O. anthropi Ochrobactrum anthropi Achromobacter VD 1,2
O. ureolytica Oligella ureolytica N/A
O. urethralis Oligella urethralis N/A
Oligella sp. Oligella species N/A
Oligella ureolytica N/A
P. yeei Paracoccus yeei (CDC group EO-2) CDC group EO-2


Ps alcal/psalc Pseudomonas alcaligenes N/A
Ps fluor/putida Pseudomonas fluorescens N/A
P. mendocina Pseudomonas mendocina N/A
P. stutzeri Pseudomonas stutzeri N/A
Pseudomonas fluorescens N/A
Pseudomonas luteola N/A
Pseudomonas mendocina N/A
Pseudomonas oryzihabitans N/A
Pseudomonas stutzeri N/A
R. pickettii Ralstonia pickettii Burkholderia (P). picketti
Roseomonas sp. Roseomonas species N/A
S. putrefaciens Shewenella putrefaciens Pseudomonas putrefaciens
S. paucimobilis Sphingomonas paucimobilis Pseudomonas paucimobilis
S. multivorum Sphingobacterium multivorum N/A
S. spiritivorum Sphingobacterium spiritivorum N/A


Sphingobact sp. Sphingobacterium species N/A
Sphingobacterium multivorum N/A
Sphingobacterium spiritivorum N/A
W. virosa Weeksella virosa N/A
information:
Special Characteristics are unique features of an organism which may help identify a particular
species. The following table lists special characteristics used in conjunction with the Rapid Neg
ID 4 gram-negative identification panels.
Table 9-C: Special characteristics

BC Brown colony S. maltophilia
S. putrefaciens
S. spiritivorum
HEM Hemolytic K. kingae
B. parapertussis
PKP Pink pigment Roseomonas sp.
PO Potato odor S. odorifera 1 & 2

Table 9-C: Special characteristics (continued)

S. multivorum
S. spiritivorum
RVP Red pigment variable S. marcescens
YBC Yellow-brown colony B. zoohelcum
W. virosa
YP Yellow pigment B. cepacia complex
C. indologenes
E. hermannii
C. sakazakii
E. vulneris
S. paucimobilis
YPL Yellow pigment luminescent P. luminescens
YWC Yellow wrinkled colony P. luteola
P. oryzihabitans
P. stutzeri
Footnotes
One or more of the footnotes listed below might be displayed to the right of the biotype number in
the FTN column.
NOTE:
Disregard footnotes A, C, D, E, G, H, K, O, P and T as they are NOT applicable for Rapid Neg
ID 4 panels.
B. Proteus mirabilis usually have MICs of <8 mcg/mL to ampicillin, Proteus vulgaris and
Morganella morganii usually have MICs of >8 mcg/mL to ampicillin.


colorless.
J. This taxon includes Pseudomonas stutzeri, Pseudomonas mendocina and CDC group VB-3.
These species may be differentiated as follows:
Wrinkled
Organism Starch Arginine colonies
P. stutzeri + - +
P. mendocina - + -
CDC VB-3 + + +
tests listed.
N. Most hemolytic strains of Acinetobacter species, whether glucose positive or glucose-

negative, belong to Genomospecies 4, and are now designated Acinetobacter haemolyticus.

Setup a new dried overnight panel at 25°C

Y. intermedia + + +

Or, setup offline tests at 25°C

Y. intermedia 100 100 80 100
Arizona, California, and Colorado. If colony morphology, specimen source, and your location
in an endemic area suggest a Yersinia pestis infection, it is important to use necessary safety
precautions as well as contact your State Health Department and the physician immediately.

Lecithinase
Organism OF Mannitol (Egg Yolk Agar 25°C)
P. putida 19 0
U. Cedecea sp. 3/5 includes Cedecea species 3 and Cedecea species 5. These species may
Organism ARG LYS SOR

Cedecea sp. 3 100 0 0
Cedecea sp. 5 50 50 100
V. D. acido/C. test includes Delftia acidovorans and Comamonas testosteroni.

Organism OMT OF Fructose

D. acidovorans 100 100
C. testosteroni 0 0

W. Citrobacter freundii complex includes: Citrobacter freundii, Citrobacter braakii, Citrobacter

youngae, Citrobacter werkmanii, Citrobacter sedlakii, Citrobacter rodentium, Citrobacter
gillenii, and Citrobacter murliniae.These species may be differentiated by the following tests:
Dulcitol
Organism SUC MEL fermentation MLN ORN H2S
C. freundii 89 100 11 11 0 78
C. braakii 20 10 85 5 5 65
C. youngae 7 80 33 0 93 60
C. werkmanii 0 0 0 100 0 100
C. sedlakii 0 100 100 100 100 0
C. rodentium 0 0 0 100 100 0
C. gillenii 33 67 0 100 0 67
C. murliniae 33 33 100 0 0 67
X. Morax/Psychr spp includes: Moraxella osloensis, Moraxella nonliquefaciens and

Psychrobacter phenylpyruvicus. These species may be differentiated by the following tests:
Organism Urea Sodium acetate alkalinization

M. osloensis 0 100
M. nonliquefaciens 0 0
P. phenylpyruvicus 100 43
Y. Ps alcal/psalc includes Pseudomonas alcaligenes and Pseudomonas pseudoalcaligenes.

Organism Growth at 42C Acid from Fructose

P. alcaligenes 0 0
P. pseudoalcaligenes 94 100
Z. Kluyvera sp. includes Kluyvera ascorbata and Kluyvera cryocrescens. These species may
Organism LYS Ascorbate Test (48 hrs) Acid from Dulcitol

K. ascorbata 100 97 45
K. cryocrescens 50 0 0

AA. Prt vulg/penneri includes Proteus vulgaris and Proteus penneri. These species may be
differentiated by the following test:
Organism IND
P. vulgaris 98
P. penneri 0
BB. Kingella sp. includes Kingella denitrificans and Kingella kingae. These species may be
Organism MAL NIT

K. denitrificans 0 93
K. kingae 99 4
DD. There are no good separating test for species within this genus (To be used with
Roseomonas sp. or Myroides sp.).
EE. Leminorella sp. includes Leminorella grimontii and Leminorella richardii. These species may
Organism MR CIT
L. richardii 0 0
FF. N. elon/weav/zoo includes Neisseria elongata, Neisseria weaveri and Neisseria

zoodegmatis. These species may be differentiated by the following tests:
Organism ARG GLU CAT

N. elongata 0 23 0
N. weaveri 0 0 100
N. zoodegmatis 0 100 0

GG. Vib fluv/furn includes Vibrio fluvialis and Vibrio furnissii. These species may be
Organism GGL
V. fluvialis 0
V. furnissii 100
HH. Aer hyd/trot/ver includes Aeromonas hydrophila, Aeromonas trota,Aeromonas veronii

biovar veronii, and Aeromonas veronii biovar sobria. These species may be differentiated by
the following tests:
DL-
Organism ARG CEL LIpase Lactate ORN ESC
A. hydrophila 99 0 99 80 0 90
A. trota 99 99 0 88 0 0
A. veronii 0 80 90 0 99 99
biovar veronii
A. veronii 99 20 92 0 0 0
biovar sobria
II. Ppneu/Aure/Mhaem includes Pasteurella pneumotropica, Actinobacillus ureae and

Mannheimia haemolytica. These species may be differentiated by the following tests:
Organism ORN XYL URE

P. pneumotropica 100 95 96
A. ureae 0 0 100
M. haemolytica 0 100 0
JJ. Alsp/Ac. Xyl/Cpau includes Achromobacter xylosoxidans, Achromobacter denitrificans,

Alcaligenes faecalis, and Cupriavidus pauculus. These species may be differentiated by the
following tests:
Organism URE NIT XYL

A. xylosoxidans 0 100 99
A. denitrificans 0 100 0
A. faecalis 2 0 0
C. pauculus 100 11 0

KK. Ac baum/haem includes Acinetobacter baumannii complex and Acinetobacter

haemolyticus. These species may be differentiated by the following tests:

MM. V. para/algino includes Vibrio alginolyticus and Vibrio parahaemolyticus.These species

may be differentiated by the following tests:
VP
Organism (1%NaCI) ARA SUC
V. alginolyticus 95 1 99
V. parahaemolyticus 0 80 1
End of footnotes

Additional tests
LabPro software selects additional tests to assist with the identification of the top 3 choices.
The results listed for each test are expressed as percent probability of a positive result.
The following table lists the additional tests:
Table 9-D: Additional tests for Rapid Neg ID 4 panels

OXI Oxidase
GLU D-Glucose Fermentation
OGL OF Glucose
OLA OF Lactose
OMA OF Maltose
OMT OF Mannitol
OFS OF Sucrose
OXY OF Xylose
MR Methyl Red
CIT Simmons Citrate
ARG Arginine Hydrolysis

Table 9-D: Additional tests for Rapid Neg ID 4 panels (continued)

DNA DNAse (25°C)
MLN Malonate Utilization
SOR D-Sorbitol Fermentation
NIT Nitrate Reduction to Nitrite
CAT Catalase
42G Growth at 42°C
FLG Flagella: Polar (PO), Peritrichous (PE), None (N).
N2 Gas from Nitrate
3FL >3 Flagella
IND Indole

Tests included on Rapid Neg ID 4 Neg panels
Table 9-E: Tests included on Rapid Neg ID 4 Neg panels

MeU* - α - D - Galactopyranoside AGAL
D-Gluconic Acid DGA
Decarboxylase Base DCB
MeU-α-D-Glucopyranoside AGL
Fructose FRU
Lysine LYS
MeU- α-L-Arabinopyranoside ALAR
Galactose GAL
Ornithine ORN, ORN2, ONB
MeU-β-D-Galactopyranoside BGAL
Glucose GLU
N-Glutaryl-Glycyl-Arginine-AMC** GGA
MeU-β-D-Glucopyranoside BGL
Glycerol GLYC
γ-L-Glutamic Acid-AMC GGLT
MeU-β-D-Glucuronide BGLR
Lactose LAC
Glycyl-L-Proline-AMC GLPR
MeU-β-D-N, N’-Diacetylchitobioside CHB
Mannitol MAN
Glycine-AMC GLY, GLY2
MeU-N-Acetyl-β-D-Glucosaminide NAG
Sorbitol SOR
L-Proline-AMC PRO
MeU-N-Acetyl-β-D-Galactosaminide NGAL
Sucrose SUC
L-Pyroglutamic Acid-AMC PYG
MeU-Phosphate, Acid pH PHO1

Table 9-E: Tests included on Rapid Neg ID 4 Neg panels (continued)

Trehalose TRE
L-Tryptophan-AMC TRYP
MeU-Phosphate, Alkaline pH PHO2
L-Arginyl-Arginine-AMC AARG, AARG2
L-Tyrosine-AMC TYR, TYR2
Urea URE
L-Arginine-AMC ARG, ARG2
5-Keto-D-Gluconic Acid KETO
* 7-Amido-4-methylcoumarin
** 4-Methylumbelliferyl
More information
• See Rapid Neg ID 4: Additional tests for the separation of gram-negative aerobic bacilli
(page 187) for a complete data table that shows the expected result for each test listed with
each organism in the database.

10
Rapid Pos ID 2 panels:
Gram-positive aerobic bacteria
• Rapid gram-positive taxa/group-level identifications (page 147)
• Additional test table for Staph/related genera (Micrococcaceae) (page 157)
• Additional test tables for Streptococcaceae (page 158)
• Tests included on Rapid Pos ID 2 panels (page 162)
• Additional test table and footnote references (page 164)

144 Rapid Pos ID 2 panels: Gram-positive aerobic bacteria
Overview
Gram-positive organisms are characterized on the basis of Gram morphology, colonial and
cellular morphology and the catalase test results.
The Taxa for Staph/related genera (Micrococcaceae) section contains the catalase-positive
genera Staphylococcus, Dermacoccus, Kocuria, Kytococcus, Macrococcus, Micrococcus,
Listeria,and the catalase-variable Rothia species.
The Taxa for Streptococcaceae (page 145) section contains the catalase-negative genera
Streptococcus, Enterococcus, Aerococcus, Leuconostoc, Pediococcus, Gemella, Abiotrophia,
and Granulicatella as well as Rhodococcus equi (catalase-positive).
Taxa in database
Taxa for Staph/related genera (Micrococcaceae)
Table 10-A: Staph/related genera (Micrococcaceae)

K. kristinae Kocuria kristinae Micrococcus kristinae
K. sedentarius Kytococcus sedentarius Micrococcus sedentarius
L. innoc/seelig Listeria innocua N/A
Listeria seeligeri N/A
L. monocytogenes Listeria monocytogenes N/A
M. caseolyticus Macrococcus caseolyticus Staphylococcus caseolyticus
Micro/Rel spp. Micrococcus and Related Species N/A
Dermacoccus nishinomiyaensis Micrococcus nishinomiyaensis
Kocuria rosea Micrococcus roseus
Kocuria roseus
Kocuria varians Micrococcus varians
Micrococcus luteus N/A
Micrococcus lylae N/A
Rothia species Rothia mucilaginosa Stomatococcus mucilaginosus
Rothia denticariosa N/A
S. aureus Staphylococcus aureus N/A
S. auricularis Staphylococcus auricularis N/A

Rapid Pos ID 2 panels: Gram-positive aerobic bacteria 145
Table 10-A: Staph/related genera (Micrococcaceae) (continued)

S. capitis Staphylococcus capitis N/A
S. cohnii Staphylococcus cohnii N/A
S. epidermidis Staphylococcus epidermidis N/A
S. haemolyticus Staphylococcus haemolyticus N/A
S. hominis Staphylococcus hominis N/A
S. hyicus Staphylococcus hyicus N/A
S. intermedius Staphylococcus intermedius N/A
S. lugdunensis Staphylococcus lugdunensis N/A
S. saprophyticus Staphylococcus saprophyticus N/A
S. schleiferi Staphylococcus schleiferi N/A
S. sciuri Staphylococcus sciuri N/A
S. simulans Staphylococcus simulans N/A
S. warneri Staphylococcus warneri N/A
S. xylosus Staphylococcus xylosus N/A
Taxa for Streptococcaceae
Table 10-B: Streptococcaceae

A. urinae Aerococcus urinae N/A
A. viridans Aerococcus viridans N/A
Abiotroph/Granul Abiotrophia/Granulicatella Nutritionally deficient

or variant streptococci
Abiotrophia species N/A
Granulicatella species N/A
E. avium Enterococcus avium Group D enterococcus

St. avium
E. casseliflavus Enterococcus casseliflavus Group D enterococcus
E. durans/hirae Enterococcus durans Group D enterococcus
Enterococcus hirae N/A

Table 10-B: Streptococcaceae (continued)

E. faecalis Enterococcus faecalis Group D enterococcus
St. faecalis
E. faecium Enterococcus faecium Group D enterococcus

St. faecium
E. gallinarum Enterococcus gallinarum Group D enterococcus
E. raffinosus Enterococcus raffinosus Group D enterococcus
Gemella species Gemella haemolysans Neisseria haemolysans
Gemella morbillorum Streptococcus morbillorum
Peptostreptococcus morbillorum
Leuconostoc spp. Leuconostoc species N/A
Pediococcus spp. Pediococcus species N/A
Rhodococcus equi Rhodococcus equi N/A
S. agalactiae Streptococcus agalactiae Group B streptococcus
S. anginosus Streptococcus anginosus Anginosus group (streptococci)
Milleri group (streptococci)
Small colony (beta streptococci)
S. bovis grp. Streptococcus bovis group Group D Streptococcus

non-enterococcus
S. constellatus Streptococcus constellatus Anginosus group (streptococci)
S. dysgal ssp. dys Streptococcus dysgalactiae ssp. dysgalactiae Streptococcus dysgalactiae
S. equi ssp. equi Streptococcus equi ssp equi Streptpcoccus equi
S. iniae Streptococcus iniae N/A
S. intermedius Streptococcus intermedius Anginosus group (streptococci)
S. mitis/oralis Streptococcus mitis S. mitis group
Streptococcus oralis Viridans group (streptococci)

Table 10-B: Streptococcaceae (continued)

S. mutans Streptococcus mutans Viridans group (streptococci)
S. parasanguinis Streptococcus parasanguinis Streptococcus parasanguis
S. pneumoniae Streptococcus pneumoniae Pneumococcus
S. pyogenes Streptoccocus pyogenes Group A (streptococci)
S. salivarius Streptococcus salivarius Viridans group (streptococci)
S. sanguinis Streptococcus sanguinis Streptococcus sanguis
Rapid gram-positive taxa/group-level identifications

Rapid gram-positive taxa
Certain species of gram-positive cocci have been combined due to similar patterns observed
with the Rapid Pos ID 2 identification panel.
Table 10-C: Rapid gram-positive taxa

Taxa or group Organsim
Micrococcus and Related Species Dermacoccus nishinomiyaensis
Kocuria rosea
Kocuria varians
Micrococcus luteus
Micrococcus lylae
Rothia species Rothia denticariosa
R. mucilaginosa
Abiotrophia/Granulicatella Abiotrophia
Granulicatella species
Enterococcus durans/hirae Enterococcus durans
E. hirae
Gemella species Gemella haemolysans
G. morbillorum
Streptococcus mitis/oralis Streptococcus mitis
S. oralis
See <XREF footnotes> for tests which might be useful for differentiation.

Group-level identifications
You can select Group level identification for Staphylococci and Related Genera and/or
Streptococcaceae. When you select group level identification, if the probability of the species or
taxon level identification is low, but the sum of the probabilities of possible identifications is at
least 85%, the group level identification is reported.
The group level identification and the taxa included are listed in the following table.
Table 10-D: Group-level identifications

Micrococcus and related Genera Micrococcus and related species
Dermacoccus nishinomiyaensis
Kocuria rosea
Kocuria varians
Micrococcus luteus
Micrococcus lylae
Macrococcus caseolyticus
Kocuria kristinae
Kytococcus sedentarius
Coagulase-negative Staphylococcus S. auricularis
S. capitis
S. cohnii
S. epidermidis
S. haemolyticus
S. hominis
S. hyicus
S. schleiferi
S. sciuri
S. simulans
S. warneri
S. xylosus

Table 10-D: Group-level identifications (continued)

While coagulase positive, S. intermedius is also included in group level identification
since AST interpretive breakpoints are the same as for coagulase-negative
staphylococci.
Note:
S. aureus, S. lugdunensis and S. saprophyticus are not included in Group-level ID for
coagulase-negative staphylococci because of positive coagulase reactions or AST
requirements.
Enterococcus species E. avium
E. casseliflavus
E. durans/hirae
E. faecalis
E. faecium
E. gallinarum
E. raffinosus
Group C/G Streptococci S. dysgalactiae ssp. dysgalactiae
S.equi subspecies equi
S. mitis group S. mitis/oralis
S. parasanguinis
S. sanguinis
S. anginosus group S. anginosus
S. constellatus
S. intermedius

information:
• Additional tests (page 154) (Additional tests)
species. Below are the special characteristics used in conjunction with MicroScan Rapid
gram-positive identifications.
Table 10-E: Special characteristics

GPR Gram-positive rod L. monocytogenes
L. innocua/L. seeligeri
YP Yellow pigment E. casseliflavus
SAT Satelliting behavior Abiotrophia/Granulicatella
VR Vancomycin resistant Pediococcus
Leuconostoc
MUC Mucoid colony Rhodococcus equi
PKP Pink pigment Rhodococcus equi

Footnotes
A. Serology may be needed for confirmation.
B. Micrococcus and Related Genera may be differentiated with the following tests:
Major Furazo- Nitrate to Gelatin

Organism pigment lidone Nitrite Hydrolysis Oxidase Urease
K. kristinae PO R - - + V
M. luteus Y R - + + V
M. lylae CW R - + + -
D. nishin O R V + + +
K. rosea PR, R + - - -
OR
K. sendentarius CW, R - + - -
BY
K. varians Y R + + - +
M. caseolyticus** - S / / + -
*Symbols: Y=yellow; CW=cream-white; PR= pastel red; OR=orange red; PO=pale orange; O=orange;
BY=buttercup yellow; /= not tested or not applicable
** Macrococcus caseolyticus are larger cocci than staphylococci or micrococci.
C. Coagulase may be a useful confirmation test. S. aureus and S. intermedius are coagulase
positive while S. hyicus and S. schleiferi are coagulase variable.
D. Anginosus group streptococci include minute-colony beta-hemolytic isolates that may type as
A, C, F, or G as well as non-beta-hemolytic strains based on phenotypic and genotypic
testing.
E. Check motility and pigment production. E. faecium is non-motile and nonpigmented. See
additional test table for more information on other enterococci.
F. This organism is commonly associated with animal sources.
H. S. mitis group includes S. mitis, S. mitis/oralis, S. parasanguinis, and S. sanguinis.

I. The S. anginosus group includes S. anginosus, S. intermedius, and S. constellatus

subspecies constellatus and pharyngis.
Organism βGAL αGLU βGLU HYA NEU

S. anginosus - V + - -
S. intermedius + + V + +
S.constellatus - + - + -
ssp. constellatus
S.constellatus + + + V -
ssp. pharyngis
Legend
βGAL: β-D-Acetylgalactosaminidase
αGLU: α - Glucosidase
βGLU: β - Glucosidase
HYA: Hyaluronidase
NEU: Neuraminidase
K. E. durans/hirae includes E. durans and E. hirae.
Organism SUC RAF

E. durans - -
E. hirae + +
Legend
SUC: Sucrose fermentation (Heart Infusion Broth)
RAF: Raffinose fermentation (Heart Infusion Broth)
O. Listeria innocua/seeligeri includes L. innocua and L. seeligeri. These species may be

differentiated from each other and L. monocytogenes with the following tests:
Organism Beta-hemolysis D-Xylose

L. innocua - -
L. seeligeri + +
L. monocytogenes + -

P. Micrococcus and Related Species includes Dermacoccus nishinomiyaensis, Kocuria rosea,

Kocuria varians, Micrococcus luteus and Micrococcus lylae.

D. nishinomiyaensis Orange V + + +
K. rosea Pale Red- + - - -
Red Orange
K. varians Yellow + - - +
M. luteus Yellow - + + V
M. lylae Cream white - + - -
Legend
NIT: Nitrate reduction to nitrite
M-OX: Modified oxidase
NT: Growth on inorganic nitrogen agar
URE: Urease
Q. Rothia species includes R. denticariosa and R. mucilaginosa.
Organism Morph ESC Colony type

R. denticariosa Rod + Spoke wheel
R. mucilaginosa Cocci + Adherent or sticky
Legend
Morph: Predominant cellular morphology on Gram stain from broth
ESC: Esculin hydrolysis
R. Abiotrophia/Granulicatella includes the nutritionally deficient or variant cocci of the

Abiotrophia and Granulicatella species.
Organism ARG βGUR

A. defectiva - -
G. adiacens - +
G. elegans + -
Legend
ARG: Arginine dihydrolase
βGUR: β-glucuronidase

S. Gemella species includes G. haemolysans and G. morbillorum.
ALK
Organism PHOS Growth
G. haemolysans + Prefers aerobic conditions
G. morbillorum - Prefers anaerobic conditions
Legend
ALK PHOS: Alkaline phosphatase
T. Rhodococcus equi are catalase-positive pleomorphic coccobacilli. Gram stains from fluid
media frequently yield bacillary forms while coccoid forms are seen from solid media.
The characteristic mucoid coalescing colonies are generally not apparent until 48 to 72
hours.
A pale or salmon pink pigment usually develops after several days of incubation on non-
selective media. R. equi is biochemically inactive on sugars but is urease and nitrate
positive.
End of footnotes
Additional tests
first organism is below 85% (low probability). Up to three tests will be printed.
The results for each test are expressed as positive (+), negative (-) or variable (v) reactions.
Below is a list of the additional tests used for gram-positive aerobic bacteria on Rapid Pos ID 2
panels.
Additional tests for Staph/related genera (Micrococcaceae)

Test
Abbreviation Test
COAG Coagulase
CP Colony pigment


Test
Abbreviation Test
NIT Nitrate reduction to nitrite
ORN Ornithine decarboxylase
Additional tests for Streptococcaceae
Additional tests: Streptococcaceae

Test
Abbreviation Test
BE Bile esculin
BS Bile solubility
CAMP CAMP test
CP Colony pigment

Additional tests: Streptococcaceae (continued)

Test
Abbreviation Test
HEM Beta hemolysis
SAT Satellite test
SUC Sucrose fermentation
Note: fermentation test results for Streptococcaceae are from 1% sugar

solutions in heart infusion broth.

Additional test table for Staph/related genera (Micrococcaceae)

Table 10-F: Additional tests for the Staph/related genera (Micrococcaceae)
Organism. COAG NOV PB ORN PYR FUR M-OX URE CP ALK NIT MNS ESC VP BAC ARG
K. kristinae / / / / / R + V + / / / + / S -
K. sedentarius / / / / / R - - + / / / - / / +
L. monocytogenes / / / / / / / / / / / + + + / /
L. innocua/seeligeri / / / / / / / / / / / V / / / /
M. caseolyticus - S S - + S + - - + + - - - R /
Micro/Rel sp. / / / / - R + V V / / / / / S -
Rothia sp. - / / / - V - / - / / / / / / /
S. aureus + S R - - S - + / + + + - + R /
S. auricularis - S S - + S - - / - V - - - R /
S. capitis - S S - - S - - / - V + - V R /
S. cohnii - R S - - S - V / V - V - V R /
S. epidermidis - S R - - S - + / V + V - + / /
S. haemolyticus - S S - + S - - / - + - - + / /
S. hominis - S/R S - - S - + / - V - - V R /
S. hyicus V S R - - S - V / + + + - - / /
S. intermedius + S S - + S - + / + + + - - R /
S. lugdunensis - S R + + S - V / - + + - + / /
S. saprophyticus - R S - - S - + / - - - - + / /
S. schleiferi - S S - + S - - / + + + - + / /
S. sciuri - R S - - S + - / + + V + - R /
S. simulans - S S - + S - + / V + V - V / /
S. warneri - S S - - S - + / - V - - + / /
S. xylosus - R S - + S - + / V V + V V R /

Legend
+ Positive / Not tested, or not applicable
- Negative S Susceptible
V Variable R Resistant
Additional test tables for Streptococcaceae

Table 10-G: Additional tests for catalase negative gram-positive cocci
AR BGU
Organism.
PYR LAP ESC BE NACL VA SAT G R MORPH
A. defectiva + + - - - S + - - Chains
A. urinae - + V - + S - - + Clusters (tetrads)
A. viridans + - V V + S - / / Clusters (tetrads)
Enterococcus sp. + + + + + V - V / Chains
Gemella sp. + + - - - S - / / Pairs and Chains
G. adiacens + + / - - S + - + Chains
G. elegans + + / - - S + + - Chains
Lactococcus* V + + + V S - / / Chains
Leuconostoc - - V V + R - / / Chains
Pediococcus - + + + V R - / / Clusters
Streptococcus sp. V + V V V S - / / Chains
Vagococcus* + + + + V S - / / Chains
*Not in current database
Legend

Non-β hemolytic streptococci

Table 10-H: Additional tests for non-β hemolytic streptococci
Organism GRP CAMP BS OPT VP ARG ESC URE SOR MAN HEM
S. agalactiae B + - R / / / / / / Weak beta,
S. bovis grp. D - - + - + - - + Gamma, alpha
S. anginosus grp. A,C,G,F or Neg - - R + + V - - - Beta, alpha,
S. mitis grp. H - - R - V V - V - Alpha
S. mutans / - - R + - V - + + Alpha, rare beta
S. pneumoniae / - + S / / / / / / Alpha
S. salivarius / - - R + - V V - - Gamma, alpha
Legend

β Hemolytic streptococci
Table 10-I: β Hemolytic streptococci
Colony
Organism SOR
GRP Size VP CAMP PYR TRE Host
S. pyogenes A Large - - + / - Humans
S. agalactiae B Large - + - / - Humans
S. dysgalactiae ssp. equismilis C, G Large - - - + - Humans
S. dysgalactiae ssp. dysgalactiae C, L Large - - - + V Animals
S. canis* G Large - + - - - Animals
S. equi ssp. equi C Large - - - - - Animals
S. equi ssp. zooepidemicus C Large - - - - + Animals
S. iniae None Large - + + - Fish**
S. porcinus* E, P, U, V Large + + + + + Swine**

(may cross react
with commercial
B sera)
Anginosus Group*** A, C, G, F, none Small + - - + - Humans

(<0.5mm at
24 hours)
* Not in current database.

** Human infections associated with handling of non-human host.
***See Additional tests for non-β hemolytic streptococci (page 159) for more information)
Legend

Enterococci
Table 10-J: Enterococci
Organism M30 CP ARG SOR MAN ARA RAF PRV SUC MGP
E. avium - - - + + + - + + V
E. durans - - + - - - - - - -
E. casseliflavus + + + V + + + V + +
E. faecalis - - + + + - - + + -
E. faecium - - + V + + V - + -
E. gallinarum + - + - + + + - + +
E. hirae - - + - - - + - + -
E. raffinosus - - - + + + + + + V
Legend

Tests included on Rapid Pos ID 2 panels
Table 10-K: Tests included on Rapid Pos ID 2 panels

L-Arginyl-L-Arginine 7-AMC AARG, AAR2
4-MeU-α-D-Galactopyranoside AGAL
4-MeU-α-D-Glucopyranoside AGL
L-Arginine 7-AMC ARG, ARG2
4-MeU-β-D-Fucoside BDFU
4-MeU-β-D-Galactopyranoside BGAL
4-MeU-β-D-Glucopyranoside BGL
4-MeU-β-D-Glucuronide BGLR, BGR2
Cellobiose CEL
4-MeU-β-D-Cellobioside CELB
4-MeU-β-D-N, N-Diacetylchitobioside CHB
L-Citrulline 7-AMC CIT, CIT2, CIT3
Fructose FRU
Glycyl-L-Proline 7-AMC GLPR
Glucose GLU
Glycine 7-AMC GLY
Glycerol GLYC
Lactose LAC
Maltose MAL, MAL2
Mannitol MAN
Melibiose MEL
Mannose MNS
4-MeU-N-Acetyl-β-D-Glucosamine NAG
4-MeU-Phosphate, acid pH PHO1, PH1A
4-MeU-Phosphate, alkaline pH PHO2, PH2A
L-Proline 7-AMC PRO

Table 10-K: Tests included on Rapid Pos ID 2 panels

Protein, Yeast Extract PYET
L-Pyroglutamic Acid -AMC PYG, PYG2
Raffinose RAF
Salicin SAL
Sorbitol SOR
Sucrose SUC
Trehalose TRE
L-Tryptophan 7-AMC TRYP, TRP2
L-Tyrosine 7-AMC TYR
Urea UREB
More information

Additional test table and footnote references

Note that these references apply to for Rapid gram-positive additional test table and footnotes.
• Murray, P. R., et al (ed), Manual of Clinical Microbiology, 8th ed. American Society for
Microbiology, Washington D.C. 2003.
• Murray, P. R., et al (ed), Manual of Clinical Microbiology, 7th ed. American Society for
Microbiology, Washington D.C, 1999
• Facklam, R., What Happened to the Streptococci: Overview of Taxonomic and Nomenclature
Changes. Clin. Micro. Rev. 15:613-630, 2002.
• Facklam, R., J.A. Elliott, Identification, Classification, and Clinical Relevance of Catalase-
Negative, Gram-Positive Cocci, Excluding the Streptococci and Enterococci. Clin. Micro.
Rev. 8:479-495, 1995.
• Facklam, R., M.D. Collins, Identification of Enterococcus species Isolated from Human
Infections by a Conventional Test Scheme. J. Clin. Microbiol. 27:731-734, 1989.
• Prescott, J.F., Rhodococcus equi: an Animal and Human Pathogen. Clin. Micro.Rev. 4:20-34,
1991.
• Ruoff, K.L., Miscellaneous Catalase-Negative, Gram-Positive Cocci: Emerging Opportunists.

J. Clin. Microbiol. 40:1129-1133, 2002.

11
References
• Probability of acceptable identification (page 166)
• Test abbreviations and definitions (page 167)
• About large format data tables (page 174)
• Rapid Neg ID 3: Additional tests for the separation of gram-negative aerobic bacilli (page 175)
• Dried Neg: Additional tests for the separation of gram-negative glucose fermenters (page 179)
• Dried Neg: Additional tests for the separation of gram-negative glucose nonfermenters/slow glucose
fermenters (page 182)
• Anaerobes: Additional identification tests for anaerobic nonspore-forming gram-negative bacilli

(page 185)
• Rapid Neg ID 4: Additional tests for the separation of gram-negative aerobic bacilli (page 187)

166 References
Probability of acceptable identification

The normalized likelihood (probability) is derived for a list of organisms based upon the
probability of a positive reaction for each test. Five or less organisms with the highest likelihood
values within a predetermined cut-off value are evaluated for the percent probability. This
determination is based upon adding the likelihoods and then dividing each organism’s likelihood
by the sum total.
Probability Category Description

 85% Species identification, Identification to species or taxon level with high
High probability probability, no additional tests required.
85% Species identification, Identification to species or taxon level with low probability,
Low probability additional tests required to confirm identification.
VRB Very Rare Biotype Agreement with expected results for the most likely taxon
insufficient to assign an identification. Repeat test or
perform alternate method of identification.
Group ID 85% Genus/Group Identification to species or taxon Identification level

(i.e. 85%), but the sum of the probabilities for the
members of the same genus/group is 85%.

References 167
Test abbreviations and definitions

The following table lists the test abbreviations and definitions for terms used this guide. Items
with multiple definitions might be applied differently for specific tests, substrates, or panel types.
Each section of this guide describes the appropriate use for the specific panel.
Click any of the following links to scroll to a specific section of the table.
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z
Table 11-A: Test abbreviations and definitions

Abbreviation Definition
Numeric
3FL >3 Flagella
42G Growth at 42°C
ACE Acetamide Utilization
AFU p-nitrophenyl-α-L-fucopyranoside
AGAL p-nitrophenyl-α-D-galactopyranoside
MeU-α-D-Galactopyranoside
AGL MeU**-α-D-Glucopyranoside
p-nitrophenyl-α-D-glucopyranoside
Arginine Hydrolysis
L - Arginine - AMC
L - Arginine -β-naphthylamide

168 References
Table 11-A: Test abbreviations and definitions (continued)

BE Bile esculin
BGAL p-nitrophenyl-β-D-galactopyranoside
BGL o-nitrophenyl-β-D-glucopyranoside
BL Beta-Lactamase
BPO4 bis-p-nitrophenyl-phosphate
BS Bile solubility
CAMP CAMP test
CAT Catalase
CE Cellobiose assimilation
CET Cetrimide, or Growth on Cetrimide Agar
CF8 Resistance to Cephalothin, MIC > 8 µg/mL
COAG Coagulase
CP Colony pigment
CS Cycloheximide sensitivity, as determined by growth or no growth on

medium containing 0.5% cycloheximide
DCB Decarboxylase base
DGA D-Gluconic Acid
DNA DNAse (25°C)

References 169

DUL Dulcitol Fermentation
ER Erythritol assimilation
Fd64 Resistance to Nitrofurantoin, MIC > 64 µg/mL
FG Fermentation of glucose with gas production
FLG Flagella: Polar, Peritrichous, None
FRU Fructose
FS Fermentation of sucrose with gas production
GAL o-nitrophenyl-β-D-galactoside
GE Germ tube formation at 35°C in 2.5-3.0 hours
GGLY Glycylglycine-β-naphthylamide
GLU Glucose
Glucose Fermentation
GLY Glycine-β-naphthylamide
H2S H2S Production, Hydrogen Sulfide (TSI)
HEM Beta hemolysis

170 References

IN Inositol assimilation
IND Indole
Indole Production
N/A N/A
K4 Resistance to Kanamycin, MIC > 4 µg/mL
KN Nitrate utilization
LA Lactose assimilation
LAC Lactose
Lactose Fermentation
LEC Lecithinase (Egg Yolk Agar, 25°C)
LEU L-Leucine-β-naphthylamide
LYA L-Lysine-β-naphthylamide (acid)
LYB L-Lysine-β-naphthylamide (alkaline)
MA Maltose assimilation
MAL Maltose
Malonate Utilization, or Maltose Fermentation
MEL Melibiose Fermentation, or Oxidation of Melibiose

(check MEL well on MicroScan panel)

References 171

MET DL-Methionine-β-naphthylamide
MLN Malonate Utilization Test
Note that the malonate test on the dried overnight panel cannot be
substituted for the Malonate Utilization Test.
MNP p-nitrophenyl-α-D-mannopyranoside
MR Methyl Red
N2 Gas from Nitrate
NGL N-γ-L-Glutamyl-β-Naphthylamide
NGLU p-nitrophenyl-n-acetyl-β-D-glucosaminide
NIT Nitrate Reduction, or Nitrate reduction to nitrite
NO2 Nitrate
NO3 Nitrite
OF/G Oxidation of Glucose
OFS OF Sucrose
OGL OF Glucose
OLA OF Lactose
OMA OF Maltose
OMT OF Mannitol
ONPG β-galactosidase
ORN Ornithine
Ornithine Decarboxylase

172 References

OXI Oxidase
OXY OF Xylose
P4 Resistance to Penicillin, MIC > 4 µg/mL
PG Pink to red pigment produced
PO Phenol oxidase enzymes present resulting in pigment production on caffeic

acid or bird seed agar
PO4 p-nitrophenyl-phosphate
PRO L-Proline-β-naphthylamide
L - Proline - AMC
L-Prolyl-β-naphthylamide
L-Pyrrolidonyl-β-Naphthylamide
N/A N/A
RA Raffinose assimilation
SAL Salicin Fermentation
SAT Satellite test
SOR Sorbitol Fermentation
ST Starch Hydrolysis
STA Starch
SU Sucrose assimilation
SUC Sucrose

References 173

Sucrose Fermentation
To4 Resistance to Tobramycin MIC > 4 µg/mL
TAR Tartrate Utilization
TDA Tryptophane Deaminase
TE MIC <4 µg/mL for Tetracycline
TR Trehalose assimilation
TRY L-Tryptophan-β-naphthylamide
URE Urea
Urea Hydrolysis, or Urease production-Christiansen Urea
VP Voges-Proskauer Test, or Acetoin production (VP broth)
N/A N/A
N/A N/A
ZAR N-α-Benzoyl-DL-Arginine-β-Naphthylamide

174 References
About large format data tables

This section includes large format data tables for the following panel types:
• Rapid Neg ID 3 panels
• Dried (Conventional) Neg panels
• Anaerobes
• Rapid Neg ID 4 panels
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175 Rapid Neg ID 3 Panels - data table: Rapid Neg ID 3: Additional tests for the separation of gram-negative aerobic bacilli
Rapid Neg ID 3: Additional tests for the separation of gram-negative aerobic bacilli
% Positive Reactions* after 2.5 hours incubation at 35°C Legend: Numbers indicate percent positive reaction, / = not tested
Table 11-B: Additional Tests for the Separation of Gram-Negative Aerobic Bacilli
Organism Abbr. OXY GLU OGL OLA OMA OMT OFS OXY 0%N 1%N 6%N 8%N 10N VNI GGL MR VP H2S CIT CUR ADO ARG DNA M36 SUC LAC MAL ARA CEL INO MLN MEL RAF RHA SOR TRE XYL NIT CAT 42G FLG CET MAC SS N2 GEL KCN 3FL IND
A. actinomycetem 19 99 / / / / / / 7 / 0 / / / 28 8 0 0 0 0 / / / 0 0 0 95 / / / / / / / / / 42 99 99 4 N / 5 0 0 / / / 0
A. caviae 99 99 / / / / / / 96 / / / / / 0 / 0 20 99 20 / 99 92 88 99 60 99 99 99 0 / 4 4 0 4 / / / 99 / PO / 80 / / 84 92 / 84
A. jandaei 99 99 / / / / / / 99 / / / / / 99 / 91 0 87 0 / 99 93 99 0 0 99 0 13 0 / 47 0 0 0 / / / 99 / PO / 80 / / 93 60 / 99
A. lwoffii 0 0 0 0 0 0 0 0 87 / 19 / / / / / / 0 33 26 / / / 0 / / / / / / / / / / / / / 6 99 48 N 0 78 / 0 2 / / 0
A. schubertii 99 99 / / / / / / 92 / / / / / 0 / 18 20 58 20 / 91 58 99 0 0 99 0 0 0 / 0 0 0 0 / / / 99 / PO / 80 / / 83 8 / 8
Ac baum/haem 0 0 99 75 54 0 0 98 99 / 13 / / / / / / 0 66 46 / / / 0 / / / / / / / / / / / / / 8 99 72 N 2 96 12 0 6 / / 0
Aer hyd/trot/ver 99 99 / / / / / / 99 / / / / / 84 / 57 0 84 59 / 99 90 99 72 14 98 32 50 0 / 1 1 8 0 / / / 98 / PO / 99 / / 98 49 / 98
Alsp/Ac.xyl/Rpau 99 0 25 0 0 0 0 35 99 / / / / / / / / 0 98 25 / / / 99 / / / / / / / / / / / / / 77 98 64 PE 40 99 76 / 0 / / 0
B. bronchisept 99 0 0 0 0 0 0 7 99 / 87 / / / / / / / 99 99 / 0 / 99 / / / / / / / / / / / / / 92 99 / PE 0 100 99 0 0 / / 0
B. cepacia cplx 99 0 99 96 93 99 53 93 99 / 7 / / / / / / 0 99 78 / 0 0 99 / / / / / / / / / / / / / 50 99 64 PO 66 91 24 0 45 / 99 0
B. diminuta 99 0 21 0 0 0 0 0 99 / 21 / / / / / / 34 1 13 / 0 14 99 / / / / / / / / / / / / / 3 98 38 PO 0 99 0 0 68 / 0 0
B. gladioli 47 / 99 37 0 99 0 99 99 / 10 / / / / / / 0 98 58 / 2 / / / / / / / / / / / / / / / 43 98 9 PO 4 99 4 0 12 / / 0
B. pseudomallei 99 0 99 99 99 99 70 99 99 / 12 / / / / / / 0 81 21 / 99 / 99 / / / / / / / / / / / / / 99 99 99 PE 10 99 39 99 79 / / 0
B. vesicularis 98 0 87 0 94 0 0 27 95 / 23 / / / / / / 49 1 2 / 0 0 99 / / / / / / / / / / / / / 5 / 19 PO 0 43 1 0 25 / 0 0
B. zoohelcum 99 0 0 0 0 0 0 0 15 / 0 / / / / / / 0 0 99 / 99 0 0 / / / / / / / / / / / / / 0 99 10 N / 2 0 0 98 / / 98
C. amalonaticus 0 99 / / / / / / 99 / / / / / 97 99 0 5 95 85 0 85 0 95 9 35 99 99 99 0 1 0 5 99 99 99 99 99 / / PE / 99 / / 0 99 / 99
C. farmeri 0 99 / / / / / / 99 / / / / / 96 99 0 0 10 59 0 85 0 97 99 15 99 99 99 0 0 99 99 99 98 99 99 99 / / PE / 99 / / 0 93 / 99
C. freundii cplx 0 99 / / / / / / 99 / / / / / 95 99 0 55 70 67 / 60 / 80 23 67 99 99 66 0 / 49 12 99 99 99 99 98 / / / / / / / 0 85 / 33
C. indologenes 96 0 98 0 98 10 14 31 99 / 0 / / / / / / 1 3 42 / / 4 0 / / / / / / / / / / / / / 22 99 42 N / 63 0 0 78 / / 98
C. koseri 0 99 / / / / / / 99 / / / / / 98 99 0 0 99 75 99 80 0 95 40 50 99 99 99 0 95 0 0 99 99 99 99 99 / / PE / 99 / / 0 0 / 99
C. meningosepti 99 0 95 57 99 99 0 3 99 / 7 / / / / / / 3 12 8 / 33 99 0 / / / / / / / / / / / / / 0 99 45 N / 92 1 0 91 / / 99
C. sakazakii 0 99 / / / / / / 99 / / / / / 98 59 9 0 99 1 0 99 0 96 99 99 99 99 99 75 18 99 99 99 0 99 99 99 / / PE / 99 / / 0 99 / 11
C. violaceum 67 99 / / / / / / 99 / 8 / / / 0 / / / 68 5 / 99 / 99 26 0 3 / / / / / / / / / 0 97 97 / PO / 99 / / 86 / / 21
CDC group EO-2 99 0 99 99 9 0 0 99 64 / 36 / / / / / / 0 99 91 / 0 / 0 / / / / / / / / / / / / / 99 82 36 N 0 82 0 18 0 / / 0
Cedecea davisae 0 99 / / / / / / 99 / / / / / 70 99 50 0 95 0 0 50 0 95 99 19 99 0 99 0 91 0 10 0 0 99 99 99 / / PE / 99 / / 0 86 / 0
Cedecea lapagei 0 99 / / / / / / 99 / / / / / 99 40 80 0 99 0 0 80 0 80 0 60 99 0 99 0 99 0 0 0 0 99 0 99 / / PE / 99 / / 0 99 / 0
Cedecea neteri 0 99 / / / / / / 99 / / / / / 99 99 50 0 99 0 0 99 0 99 99 35 99 0 99 0 99 0 0 0 99 99 99 99 / / PE / 99 / / 0 65 / 0
Cedecea sp. 3/5 0 99 / / / / / / 99 / / / / / 99 99 50 0 99 0 0 75 0 99 75 0 99 0 99 0 0 99 99 0 50 99 99 99 / / PE / 99 / / 0 99 / 0
D. acido/C. test 99 0 0 0 0 50 0 0 99 / 3 / / / / / / 28 70 4 / 0 1 99 / / / / / / / / / / / / / 97 / / PO 2 99 / 0 6 / 99 0
E. aerogenes 0 99 / / / / / / 99 / / / / / 99 5 98 0 95 2 98 0 0 97 99 95 99 99 99 95 95 99 96 99 99 99 99 99 / / PE / 99 / / 0 98 / 0

Table 11-B: Additional Tests for the Separation of Gram-Negative Aerobic Bacilli (continued)
E. albertii 0 99 / / / / / / / / / / / / 99 / 0 0 0 0 0 0 0 0 0 0 60 99 0 0 / 0 0 0 0 60 0 99 99 / / / / / / 0 0 / 0
E. americana 0 99 / / / / / / 99 / / / / / 0 84 95 0 95 0 0 0 0 60 0 70 16 0 10 0 0 0 0 23 0 99 13 97 / / PE /99 / / 0 5 / 0 0
E. amnigenus 1 0 99 / / / / / / 99 / / / / / 99 7 99 0 70 0 0 9 0 92 99 70 99 99 99 0 91 99 99 99 9 99 99 99 / / PE / 99 / / 0 99 / 0
E. amnigenus 2 0 99 / / / / / / 99 / / / / / 99 65 99 0 99 0 0 35 0 99 0 35 99 99 99 0 99 99 0 99 99 99 99 99 / / PE / 99 / / 0 99 / 0
E. asburiae 0 99 / / / / / / 99 / / / / / 95 99 2 0 99 60 0 21 0 0 99 75 99 99 99 0 3 0 70 5 99 99 97 99 / / N / 99 / / 0 97 / 0
E. brevis 99 0 99 0 99 0 0 0 99 / / / / / / / / 0 0 0 / 0 / 0 / / / / / / / / / / / / / 0 99 0 N / 99 0 0 99 / / 99
E. cancerogenus 0 99 / / / / / / 99 / / / / / 99 5 99 0 99 1 0 94 0 99 0 10 99 99 99 0 99 0 0 99 1 99 99 99 / / PE / 99 / / 0 98 / 0
E. cloacae 0 99 / / / / / / 99 / / / / / 99 59 9 0 99 65 25 97 0 95 97 93 99 99 99 15 75 90 97 92 95 99 99 99 / / PE / 99 / / 0 98 / 0
E. coli 0 99 / / / / / / 99 / / / / / 95 99 0 1 1 1 5 17 0 95 50 95 95 99 2 1 0 75 50 80 94 98 95 99 / / PE / 99 / / 0 3 / 98
E. coli-O157-H7 0 99 / / / / / / 99 / / / / / 90 99 0 1 1 1 5 17 0 95 50 95 95 99 2 1 0 75 50 80 1 98 95 99 / / PE / 99 / / 0 3 / 98
E. corrodens 99 0 0 0 0 0 0 0 71 / / / / / / / / 0 0 0 / / / 0 / / / / / / / / / / / / / 99 8 / N 0 0 0 0 0 / / 0
E. fergusonii 0 99 / / / / / / 99 / / / / / 95 99 0 0 17 0 98 5 0 93 0 0 96 98 96 0 35 0 0 92 0 96 96 99 / / PE / 99 / / 0 0 / 98
E. gergoviae 0 99 / / / / / / 99 / / / / / 98 5 99 0 99 93 0 0 0 90 98 55 99 99 99 0 96 97 97 98 0 99 99 99 / / PE / 99 / / 0 0 / 0
E. hermannii 0 99 / / / / / / 99 / / / / / 97 99 0 0 1 0 0 0 0 99 45 45 99 99 97 0 0 0 40 97 0 99 99 99 / / PE / 99 / / 0 94 / 99
E. hormaechei 0 99 / / / / / / 99 / / / / / 83 57 99 0 96 87 0 78 0 52 99 99 99 99 9 0 99 0 0 99 0 99 96 99 / / PE / 99 / / 0 99 / 0
E. tarda 0 99 / / / / / / 99 / / / / / 99 99 0 99 1 0 0 0 0 98 0 0 99 9 0 0 0 0 0 0 0 0 0 99 / / PE / 99 / / 0 0 / 99
E. vulneris 0 99 / / / / / / 99 / / / / / 97 99 0 0 0 0 0 30 0 99 8 15 99 99 99 0 85 99 99 93 1 99 99 99 / / PE / 99 / / 0 15 / 0
G. hollisae 99 99 / / / / / / 0 99 83 0 0 99 0 / / 0 / / / / / / / / / / / / / / / / / / / / / / N / / / / / / / 97
Hafnia alvei 0 99 / / / / / / 99 / / / / / 98 40 85 0 10 4 0 6 0 85 10 59 99 51 5 0 50 0 2 97 0 95 98 99 / / PE / 99 / / 0 95 / 0
K. intermedia 0 99 / / / / / / 99 / / / / / 99 99 99 0 65 0 0 0 0 89 65 99 99 99 99 0 99 99 99 99 99 99 99 99 / / PE / 99 / / 0 65 / 0
K. oxytoca 0 99 / / / / / / 99 / / / / / 97 20 95 0 95 90 99 0 0 0 99 99 99 98 99 98 98 99 99 99 99 99 99 99 / / N / 99 / / 0 97 / 99
K. ozaenae 0 99 / / / / / / 99 / / / / / 50 98 0 0 30 10 97 6 0 0 20 30 95 98 92 55 3 97 90 55 65 98 95 80 / / N / 99 / / 0 88 / 0
K. pneumoniae 0 99 / / / / / / 99 / / / / / 97 10 98 0 98 95 90 0 0 0 99 98 98 99 98 95 93 99 99 99 99 99 99 99 / / N / 99 / / 0 98 / 0
K. rhinoscler 0 99 / / / / / / 99 / / / / / 0 99 0 0 0 0 99 0 0 0 75 0 99 99 99 95 95 99 90 96 99 99 99 99 / / N / 99 / / 0 80 / 0
Kingella sp. 99 93 / / / / / / 71 / 4 / / / / / / 0 0 0 / / / / 0 0 50 / / / / / / / / / 0 48 5 29 / / 5 0 44 1 / / 0
Kluyvera sp. 0 99 / / / / / / 99 / / / / / 94 99 0 0 88 0 0 0 0 94 90 97 99 99 99 0 91 99 99 99 43 99 95 99 / / PE / 99 / / 0 89 / 91
L. adecarboxy 0 99 / / / / / / 99 / / / / / 97 99 0 0 0 48 93 0 0 79 66 93 99 99 99 0 93 99 66 99 0 99 99 99 / / PE / 99 / / 0 97 / 99
Leminorella sp. 0 99 / / / / / / 99 / / / / / 17 50 0 99 50 0 0 0 0 0 0 0 0 99 0 0 0 0 0 0 0 0 92 99 / / N / / / / 0 0 / 0
M. morganii 0 99 / / / / / / 99 / / / / / 90 95 0 20 0 95 0 0 0 95 0 1 0 0 0 0 1 0 0 0 0 0 0 90 / / PE / 99 / / 0 98 / 95
M. wisconsensis 0 99 / / / / / / 99 / / / / / 0 99 0 0 80 0 99 0 0 0 99 99 30 0 0 0 0 99 99 0 0 0 0 90 / / N / 99 / / 0 70 / 0
Morax/Psychr spp. 99 / 0 0 0 0 0 0 58 / 10 / / / / / / 0 0 0 / / / 0 / / / / / / / / / / / / / / 91 32 N / 55 9 / / / / 0
Myroides sp. 99 0 0 0 0 0 0 0 99 / 25 / / / / / / 0 0 99 / 99 / 0 / / / / / / / / / / / / / 0 99 31 N 0 96 41 0 96 / / 0
N elo/weav/zoo 99 0 33 0 0 0 0 0 90 / 5 / / / / / / 0 7 0 / / / 0 / / / / / / / / / / / / / 98 67 44 N / 57 0 60 3 / / 0

O. anthropi 99 0 99 0 64 57 50 99 99 / 60 / / / / / / 43 64 99 / 71 / 99 / / / / / / / / / / / / / 86 99 64 PE 3 99 99 43 0 / / 0
O. ureolytica 99 0 0 0 0 0 0 0 22 / 20 / / / / / / 0 30 97 / / 0 99 / / / / / / / / / / / / / 99 99 18 PE 0 89 56 0 0 / / 0
O. urethralis 99 0 0 0 0 0 0 0 96 / 59 / / / / / / 0 46 0 / / 0 0 / / / / / / / / / / / / / 0 99 59 N / 96 9 0 0 / / 99
P. aerogenes 99 99 / / / / / / 99 / / / / / 99 / / / 0 99 / 0 / 0 94 57 99 / / / / / / / / / 81 99 99 / N / 99 6 0 0 / / 0
P. aeruginosa 99 0 87 0 17 0 0 90 99 / 65 / / / / / / 0 / 57 / 99 10 97 / / / / / / / / / / / / / 98 / 99 PO 94 99 96 93 82 / 0 0
P. agglomerans 0 99 / / / / / / 99 / / / / / 20 50 70 0 50 20 7 0 0 85 75 40 89 95 55 15 65 50 30 85 30 97 93 85 / / PE / 99 / / 23 5 / 20
P. alcalifaciens 0 99 / / / / / / 99 / / / / / 85 99 0 0 98 0 98 0 0 96 15 0 1 1 0 1 0 0 1 0 1 2 1 99 / / PE / 99 / / 0 99 / 99
P. damsela 95 99 / / / / / / 0 99 95 0 0 99 10 / / 0 / / / / / / / / / / / / / / / / / / / / / / N / / / / / / / 0
P. luminescens 0 99 / / / / / / / / / / / / 0 0 0 0 20 99 0 0 0 99 0 0 0 0 0 0 / 0 0 0 0 0 0 0 / / / / 99 / / 99 60 / 0
P. luteola 0 0 99 27 99 94 12 99 99 / 74 / / / / / / / / 64 / 99 0 99 / / / / / / / / / / / / / 62 / 94 PO 0 99 68 0 61 / / 0
P. mendocina 99 / 99 0 0 0 0 99 99 / 99 / / / / / / 0 99 50 / 99 / 99 / / / / / / / / / / / / / 99 99 99 PO 99 99 99 99 0 / 0 0
P. mirabilis 0 99 / / / / / / 99 / 87 / / / 96 97 50 98 65 98 0 0 50 95 15 2 0 0 1 0 2 0 1 1 0 98 98 95 / / PE / 99 / / 90 98 / 2
P. multocida 96 99 / / / / / / 80 / / / / / 0 / / / 0 0 / 3 / 0 99 8 1 / / / / / / / / / 85 99 98 / N / 2 0 0 0 / / 98
P. oryzihabitans 0 0 99 36 97 99 25 99 99 / 62 / / / / / / / / 77 / 14 0 99 / / / / / / / / / / / / / 6 / 33 PO 53 99 22 0 17 / / 0
P. rettgeri 0 99 / / / / / / 99 / / / / / 10 93 0 0 95 98 99 0 0 94 15 5 2 0 39 0 0 5 57 0 1 0 10 99 / / PE / 99 / / 0 97 / 99
P. rustigianii 0 99 / / / / / / 99 / / / / / 35 65 0 0 15 0 0 0 0 30 35 0 0 0 0 0 0 0 0 0 0 0 0 99 / / PE / 99 / / 0 99 / 98
P. shigelloides 99 99 / / / / / / 99 / / / / / 0 90 0 0 0 0 0 98 0 95 0 80 95 0 0 95 0 70 0 0 0 99 0 99 93 / PO 0 99 96 0 0 1 / 99
P. stuartii 0 99 / / / / / / 99 / / / / / 0 99 0 0 93 30 5 0 10 85 50 2 1 1 5 95 0 0 7 0 1 98 7 99 / / PE / 99 / / 0 99 / 98
P. stutzeri 99 / 99 0 99 93 0 99 96 / 96 / / / / / / 0 96 55 / 0 / 99 / / / / / / / / / / / / / 99 99 69 PO 4 99 54 99 0 / 0 0
P. yeei (CDC EO-2) 99 0 99 99 9 0 0 99 64 / 36 / / / / / / 0 99 91 / 0 / 0 / / / / / / / / / / / / / 99 82 36 N 0 82 0 18 0 / / 0
Ppneu/Aure/Mhaem 98 99 / / / / / / 59 / / / / / 0 / / / 0 65 / 0 / 0 99 34 98 / / / / / / / / / 65 99 86 / N / 44 / 0 0 / / 30
Prt vulg/penneri 0 99 / / / / / / 99 / / / / / 70 98 25 62 32 98 0 0 45 90 57 15 0 0 1 0 1 0 1 1 0 76 98 92 / / PE / 99 / / 70 98 / 49
Ps alcal/psalc 98 0 5 0 0 0 0 15 99 / 55 / / / / / / 0 / 5 / 45 0 99 / / / / / / / / / / / / / / / 47 PO / 98 / / 0 / 0 0
Ps fluor/putida 99 0 99 31 16 39 / 99 99 / 71 / / / / / / 0 96 63 / 99 0 99 / / / / / / / / / / / / / 10 / 0 PO 88 99 97 1 / / / 0
R. ornithinolytica 0 99 / / / / / / 99 / / / / / 99 96 70 0 99 99 99 0 0 0 99 99 99 99 99 95 99 99 99 99 99 99 99 99 / / N / 99 / / 0 99 / 99
R. pickettii 99 0 99 0 0 0 0 99 99 / 3 / / / / / / 0 / 99 / 0 0 99 / / / / / / / / / / / / / 99 / 94 PO 0 77 0 99 33 / 0 0
R. radiobacter 99 0 99 99 99 99 99 99 99 / 18 / / / / / / 14 99 97 / 8 / 99 / / / / / / / / / / / / / 83 98 34 PE 0 99 25 5 2 / / 0
Roseomonas sp. 92 0 10 0 0 9 0 64 99 / 13 / / / 3 / / 0 69 99 / / / 67 / / / / / / / / / / / / / 51 99 89 / / 99 3 3 / / / 0
S. ficaria 8 99 / / / / / / 99 / / / / / 0 75 75 0 99 0 0 0 99 99 99 15 99 99 99 55 0 40 70 35 99 99 99 92 / / PE / 99 / / 99 55 / 0
S. fonticola 0 99 / / / / / / 99 / / / / / 79 99 9 0 91 13 99 0 0 91 21 97 97 99 63 0 88 98 99 76 99 99 85 99 / / PE / 99 / / 0 70 / 0
S. liquefac cplx 0 99 / / / / / / 99 / / / / / 75 93 93 0 90 3 5 0 85 95 98 10 98 98 56 0 2 75 85 15 95 99 99 99 / / PE / 99 / / 90 90 / 1
S. maltophilia 0 0 85 60 99 0 63 35 99 / / / / / / / / 95 34 3 / 0 99 99 / / / / / / / / / / / / / 39 99 48 PO 29 92 2 0 93 / 99 0
S. marcescens 0 99 / / / / / / 99 / / / / / 55 20 98 0 98 15 40 0 98 97 99 2 96 0 5 75 3 0 2 0 99 99 7 98 / / PE / 99 / / 90 95 / 1

S. multivorum 99 / 99 99 99 0 99 99 99 / 25 / / / / / / 0 0 95 / 0 / 0 / / / / / / / / / / / / / 0 99 0 / 0 99 0 0 0 / / 0
S. odorifera 1 0 99 / / / / / / 99 / / / / / 0 99 50 0 99 55 0 0 99 99 99 70 99 99 99 99 0 98 99 95 99 99 99 99 / / PE / 99 / / 95 60 / 60
S. odorifera 2 0 99 / / / / / / 99 / / / / / 13 60 99 0 97 0 55 0 99 99 0 97 99 99 99 99 0 96 7 94 99 99 99 99 / / PE / 99 / / 94 19 / 50
S. Paratyphi A 0 99 / / / / / / 99 / / / / / 99 99 0 10 0 0 0 15 0 95 0 0 95 99 5 0 0 95 0 99 95 99 0 99 / / PE / 99 99 / 0 0 / 0
S. paucimobilis 90 0 99 99 99 0 99 99 99 / 0 / / / / / / 0 0 0 / 8 0 99 / / / / / / / / / / / / / 0 99 24 PO 0 0 0 0 0 / 0 0
S. plymuthica 0 99 / / / / / / 99 / / / / / 40 94 80 0 75 0 0 0 99 50 99 80 94 99 88 50 0 93 94 0 65 99 94 99 / / N / 99 / / 60 30 / 0
S. putrefaciens 99 0 50 0 99 0 99 0 99 / 69 / / / / / / 96 8 12 / 0 99 99 / / / / / / / / / / / / / 99 99 30 PO 49 9 8 0 65 / 0 0
S. rubidaea 0 99 / / / / / / 99 / / / / / 30 20 99 0 95 2 99 0 99 85 98 99 99 99 94 20 94 99 99 1 1 99 99 99 / / PE / 99 / / 90 25 / 0
S. spiritivorum 99 / 99 99 99 99 99 99 99 / 0 / / / / / / 0 0 99 / 25 / 0 / / / / / / / / / / / / / 0 99 9 / 0 46 0 0 15 / / 0
S. Typhi 0 99 / / / / / / 99 / / / / / 0 99 0 97 0 0 0 3 0 97 0 1 97 2 0 0 0 99 2 0 99 99 82 99 / / PE / 99 99 / 0 0 / 0
S. entérica 0 99 / / / / / / 99 / / / / / 96 99 0 95 95 1 0 70 2 95 1 1 / 99 53 5 0 95 2 95 / 99 97 99 / / PE / 99 99 / 0 0 / 1
Shigella sp. 0 99 / / / / / / 99 / / / / / 1 99 0 0 0 0 0 7 0 0 1 1 39 74 1 0 0 24 11 28 26 85 5 99 / / / / 99 99 / 0 0 / 30
Tatumella 0 99 / / / / / / 99 / / / / / 0 0 5 0 2 0 0 0 0 0 98 0 0 0 0 0 0 25 11 0 0 93 9 98 / / PO / 99 / / 0 0 / 0
V. cholerae 99 99 / / / / / / 99 99 53 1 0 99 0 99 75 0 97 0 0 0 93 99 99 79 9 0 8 0 1 1 0 0 1 99 0 99 / / N / / / / 90 10 / 99
V. metschnikovi 0 99 / / / / / / 0 99 78 44 4 0 0 / / 0 / / / / / / / / / / / / / / / / / / / / / / N / / / / / / / 20
V. mimicus 99 99 / / / / / / 99 99 49 0 0 99 0 99 9 0 99 1 0 0 55 98 0 21 99 1 0 0 0 0 0 0 0 94 0 / / / N / / / / 60 2 / 98
V. para/algino 99 99 / / / / / / 0 99 99 87 36 99 0 / / 0 / / / / / / / / / / / / / / / / / / / / / / N / / / / / / / 82
V. vulnificus 99 99 / / / / / / 0 99 65 0 0 99 0 / / 0 / / / / / / / / / / / / / / / / / / / / / / N / / / / / / / 97
Vib fluv/furn 99 99 / / / / / / 0 99 98 75 2 99 / / / 0 / / / / / / / / / / / / / / / / / / / / / / N / / / / / / / 12
W. virosa 98 0 0 0 0 0 0 0 99 / 7 / / / / / / 0 0 0 / 0 3 0 / / / / / / / / / / / / / 0 98 70 N / 10 0 / 99 / / 99
Y. entero group 0 99 / / / / / / 90 / / / / / 22 97 2 0 5 76 0 0 1 4 73 22 93 93 92 20 1 20 20 50 99 99 88 98 / / N / 99 / / 0 3 / 70
Y. pestis 0 99 / / / / / / / / / / / / 0 80 0 0 0 5 0 0 0 0 0 0 80 99 0 0 0 20 0 15 0 99 90 85 / / / / 50 / / 0 0 / 0
Y. pseudotb 0 99 / / / / / / 99 / / / / / 0 99 0 0 0 95 0 0 0 0 0 0 95 50 0 0 0 70 15 70 0 99 99 95 / / N / 99 / / 0 0 / 0
Y. regensburgei 0 99 / / / / / / 99 / / / / / 99 99 0 0 92 0 0 8 0 99 0 0 99 99 99 0 0 92 25 99 0 99 99 99 99 / PE / 99 / / 0 92 / 0
Y. ruckeri 0 99 / / / / / / 99 / / / / / 59 71 0 0 0 0 0 5 0 0 0 0 95 5 5 0 0 0 5 0 50 95 0 75 / / N / 99 / / 30 15 / 0

179 Dried (Conventional) Neg panels - data tables: Dried Neg: Additional tests for the separation of gram-negative glucose fermenters
Dried Neg: Additional tests for the separation of gram-negative glucose fermenters
Table 11-C: Additional tests for the separation of gram-negative glucose fermenters
Organism Abbr. OXI 0%N 1%N 6%N 8%N 10N GGL MR H2S CUR DNA M36 LAC MAL CEL DUL SAL TRE XYL GEL KCN
Aer. hydro 99 99 / 0 / / 46 95 0 0 99 96 9 96 37 / / 99 1 78 58
Cedecea davisae 0 / / / / / 70 99 0 0 0 95 19 99 99 0 99 99 99 0 86
Cedecea lapagei 0 / / / / / 99 40 0 0 0 80 60 99 99 0 99 99 0 0 99
Cedecea neteri 0 / / / / / 99 99 0 0 0 99 35 99 99 0 99 99 99 0 65
Cedecea sp. 3 0 / / / / / 99 99 0 0 0 99 0 99 99 0 99 99 99 0 99
Cedecea sp. 5 0 / / / / / 99 99 0 0 0 99 0 99 99 0 99 99 99 0 99
C. indologenes 96 / / / / / / / 1 14 14 0 / / / / / / / 78 /
C. violaceum 67 / / / / / 0 / / 19 99 20 0 3 / / / 0 97 86 /
C. amalonaticus 0 / / / / / 95 99 0 / 0 98 / 99 99 2 / 99 99 0 95
C. koseri 0 / / / / / 98 99 0 75 0 95 35 99 99 50 20 99 99 0 0
C. freundii cplx 0 / / / / / 95 99 80 70 0 95 50 99 55 55 5 99 99 0 96
C. sakazakii 0 / / / / / 98 5 0 1 0 96 99 99 99 5 99 99 99 0 99
E. tarda 0 / / / / / / 99 / 0 0 98 0 99 0 0 0 0 0 0 0
E. meningo (F) 99 / / / / / / / 3 3 99 0 / / / / / / / 91 /
E. aerogenes 0 / / / / / 99 5 0 2 0 97 95 99 99 5 99 99 99 0 98
E. amnigenus 1 0 / / / / / 99 7 0 0 0 92 70 99 99 0 91 99 99 0 99
E. amnigenus 2 0 / / / / / 99 65 0 0 0 99 35 99 99 0 99 99 99 0 99
E. asburiae 0 / / / / / 95 99 0 60 0 0 75 99 99 0 99 99 97 0 97
E. cancerogenus 0 / / / / / 99 5 0 1 0 99 10 99 99 0 92 99 99 0 98
E. cloacae 0 / / / / / 99 5 0 65 0 95 93 99 99 15 75 99 99 0 98
E. gergoviae 0 / / / / / 98 5 0 93 0 90 55 99 99 0 99 99 99 0 0
E. coli 0 / / / / / 90 95 1 1 0 95 90 95 2 60 40 98 95 0 3
E. fergusonii 0 / / / / / 95 99 0 0 0 93 0 96 96 60 65 96 96 0 0
E. hermanii 0 / / / / / 97 99 0 0 0 99 45 99 97 19 40 99 99 0 94
E. vulneris 0 / / / / / 97 99 0 0 0 99 15 99 99 0 30 99 99 0 15
E. americana 0 / / / / / 0 84 0 0 0 60 70 16 10 0 80 99 13 0 5
G. hollisae 99 0 99 83 0 0 0 / 0 0 0 0 / / / / / / / / 0
H. alvei 0 / / / / / 98 40 0 4 0 85 5 / 15 0 10 70 98 0 95

Table 11-C: Additional tests for the separation of gram-negative glucose fermenters (continued)
K. oxytoca 0 / / / / / 97 20 0 90 0 0 99 99 99 55 99 99 99 0 97
K. ozaenae 0 / / / / / 50 98 0 10 0 0 30 95 92 2 97 98 95 0 88
K. pneumoniae 0 / / / / / 97 10 0 95 0 0 98 98 98 30 99 99 99 0 98
K. rhinoscler 0 / / / / / 0 99 0 0 0 0 0 99 99 0 98 99 99 0 80
K. ascorbata 0 / / / / / 93 99 0 0 0 98 98 99 99 25 98 99 99 0 92
K. cryocrescens 0 / / / / / 95 99 0 0 0 90 95 99 99 0 99 99 91 0 86
K. intermedia 0 / / / / / 99 99 0 0 0 89 99 99 99 99 99 99 99 0 65
L. adecarboxy 0 / / / / / 97 99 0 48 0 79 93 99 99 86 99 99 99 0 97
Leminorella sp. 0 / / / / / 33 / 99 0 0 0 0 99 30 0 0 0 0 0 70
M. wisconsensis 0 / / / / / 0 99 0 0 0 0 99 30 0 0 0 0 0 0 70
M. morganii 0 / / / / / 90 96 / 98 0 / 1 0 0 0 0 10 0 0 95
P. aerogenes 99 / / / / / 99 99 0 99 / 0 57 99 / / / / 81 0 /
P. agglomerans 0 / / / / / 20 50 0 20 0 85 40 89 55 15 65 97 93 2 35
P. damselae 95 0 99 95 0 0 10 / 0 0 75 25 / / / / / / / / 5
P. multocida 97 / / / / / 0 / / 0 / 0 8 2 / / / / 67 0 /
Past-Actin sp. / / / / / / 0 / / / / 0 / 85 / / / 50 60 1 /
P. shigelloides 96 / / / / / 0 99 0 0 4 85 65 99 0 0 0 96 0 0 2
P. mirabilis 0 / / / / / 96 97 98 98 50 95 2 0 1 0 0 98 98 90 96
P. penneri 0 / / / / / 45 99 30 99 40 85 1 99 0 0 0 55 99 50 99
P. vulgaris 0 / / / / / 85 95 95 95 80 95 2 97 0 0 50 30 95 91 99
P. alcal 1-2 0 / / / / / 85 99 0 0 0 96 0 1 1 0 1 2 1 0 99
P. rettgeri 0 / / / / / 10 93 0 98 0 94 5 2 3 0 50 0 10 0 97
P. rustigianii 0 / / / / / 35 65 0 0 0 30 0 0 0 0 0 0 0 0 95
P. stuartii 0 / / / / / 0 99 0 0 10 85 2 1 4 0 2 98 7 0 99
P. stuart UREA + 0 / / / / / 0 99 0 99 10 85 2 1 4 0 2 98 7 0 99
R. ornithinolyt 0 / / / / / 99 96 0 99 0 0 99 99 99 10 99 99 99 0 99
S. Choleraesuis 0 / / / / / 95 99 50 0 0 95 0 95 0 5 0 0 98 0 0
S. Paratyphi A 0 / / / / / 99 99 10 0 0 95 0 95 5 90 0 99 0 0 0
S. Typhi 0 / / / / / 0 99 97 0 0 97 1 97 0 0 0 99 82 0 0
S. ent arizonae 0 / / / / / 99 99 99 0 2 98 / 98 1 1 0 99 99 0 1
Salmonella sp. 0 / / / / / 96 99 95 1 2 95 2 / 5 95 3 99 97 0 0
S. liquefac cplx 0 / / / / / 75 93 0 3 85 95 10 98 5 0 97 99 99 90 90

Table 11-C: Additional tests for the separation of gram-negative glucose fermenters (continued)
S. marcescens 0 / / / / / 55 20 0 15 98 97 2 96 5 0 92 99 7 90 95
S. odorifera 1 0 / / / / / 0 99 0 5 99 99 70 99 99 0 98 99 99 95 60
S. odorifera 2 0 / / / / / 13 60 0 0 99 99 97 99 99 0 45 99 99 94 19
S. plymuthica 0 / / / / / 40 94 0 0 99 50 80 94 88 0 94 99 94 60 30
S. rubidaea 0 / / / / / 30 20 0 2 99 85 99 99 94 0 99 99 99 0 70
S. fonticola 0 / / / / / 79 99 0 13 0 91 97 97 6 91 99 99 85 0 70
S. sonnei 0 / / / / / 0 99 0 0 0 0 2 90 5 0 0 99 2 0 0
Shigella sp. 0 / / / / / 2 99 0 0 0 0 2 30 0 2 0 80 2 0 0
Tatumella 0 / / / / / 0 0 0 0 0 0 0 0 0 0 55 93 9 0 0
V. alginolyt 99 0 99 99 94 69 0 / 0 0 92 99 / / / / / / / / 15
V. cholerae 99 99 99 53 1 0 0 25 0 0 93 99 7 99 8 0 1 99 0 52 10
V. fluvialis 99 0 99 99 96 71 4 / 0 0 99 70 / / / / / / / / 65
V. mimicus 99 99 99 49 0 0 0 14 0 1 55 98 21 99 0 0 0 94 0 65 2
V. parahaemolyt 99 0 99 99 80 2 0 / 0 15 92 99 / / / / / / / / 20
V. vulnificus 99 0 99 65 0 0 0 / 0 1 50 99 / / / / / / / / 1
Y. entero group 0 / / / / / 40 92 0 / 5 5 40 75 75 0 30 98 70 0 2
Y. pestis 0 / / / / / 0 80 0 5 0 0 0 80 0 0 70 99 90 0 0
Y. pseudotb 0 / / / / / 0 99 0 95 0 0 0 95 0 0 25 99 99 0 0
Y. ruckeri 0 / / / / / 5 97 0 0 0 0 0 95 5 0 0 95 0 30 15
Y. regensburgei 0 / / / / / 99 99 0 0 0 99 0 99 99 0 8 99 99 0 92

/ = Not Tested

182 Dried (Conventional) Neg panels - data tables: Dried Neg: Additional tests for the separation of gram-negative glucose nonfermenters/slow glucose fermenters
Dried Neg: Additional tests for the separation of gram-negative glucose nonfermenters/slow glucose fermenters
Table 11-D: Additional tests for the separation of gram-negative glucose nonfermenters/slow glucose fermenters
Organism Abbr. OXI OGL OLA OMA OMT OFS OXY 0%N 6%N DNA M36 CAT 42G FLG CET MAC SS N2 GEL 3FL ST TE LEC MEL
A. lwoffii 0 0 0 0 0 0 0 97 9 0 0 99 63 N 0 90 7 0 4 / 0 83 10 /
A. xylosoxidans 99 78 0 0 0 0 99 99 69 0 99 98 84 PE 96 99 98 60 0 / 3 0 0 /
Ac baumann/haem 0 99 97 63 2 0 99 99 22 / 0 99 87 N 3 99 20 0 9 / 0 73 10 96
Alcaligenes sp. 99 0 0 0 0 0 0 99 / 0 99 98 / PE 38 99 81 / 22 / 1 75 0 /
B. bronchiseptica 99 0 0 0 1 0 7 99 82 0 99 99 78 PE 0 99 99 0 0 / 0 99 0 /
B. cepacia cplx 92 99 99 99 99 86 99 99 7 0 99 99 83 PO 44 99 6 0 20 99 1 13 48 /
B. pseudomallei 99 99 99 99 94 66 86 99 12 0 99 99 99 PO 7 99 8 99 79 99 0 99 86 /
B. zoohelcum 99 0 0 0 0 0 0 15 0 0 0 99 10 N / 2 0 / 98 / 0 99 0 /
C. indologenes 96 92 0 92 10 13 30 99 0 4 0 99 42 N / 63 0 0 78 / 99 48 0 /
C. pauculus 99 0 0 0 0 0 0 99 11 0 99 99 86 PE 0 99 3 0 0 / 20 99 0 /
D. acidovorans 99 0 0 0 99 0 0 99 7 1 99 99 3 PO 5 99 67 0 12 99 0 92 0 /
E. brevis 99 99 0 99 0 0 0 99 0 99 0 99 0 N / 99 0 0 99 / / / / /
E. meningo (NF) 99 95 42 93 91 0 2 99 7 99 0 99 45 N / 92 1 0 99 99 9 4 0 /
Morax/Psychr sp. 99 0 0 0 0 0 0 40 8 0 0 91 26 N / / 9 / / / 0 99 0 /
Myroides sp. 99 0 0 0 0 0 0 99 20 93 0 99 31 N / 96 30 / 96 / 0 13 0 /
O. anthropi 99 99 0 57 80 53 96 99 45 0 99 99 56 PE 3 99 96 99 0 / 0 84 0 /
O. ureolytica 99 0 0 0 0 0 0 19 14 0 90 99 18 PE 0 89 5 90 0 / 0 40 0 /
P. aeruginosa 99 97 1 0 70 0 90 99 65 11 96 99 99 PO 94 99 96 93 82 0 2 39 9 /
P. luteola 0 99 6 99 82 0 99 99 76 0 99 99 81 PO 0 99 67 0 52 / 50 99 0 /
P. multo SF 97 / / / / / / 99 / / 0 98 / N / 2 / 0 0 / / / / /
P. oryzihabitans 0 99 14 97 99 25 99 99 62 0 90 94 33 PO 97 99 22 0 17 / 78 97 0 3
P. stutzeri 99 96 0 99 89 0 93 96 80 0 99 99 69 PO 4 99 54 99 0 0 92 95 9 /
Past-Actin sp. SF 95 / / / / / / / / / 0 / / N / 80 / / 1 / / / / /
Ps fluor/putida 99 99 14 36 / / 98 99 / 0 99 / 0 PO 92 99 97 5 / / / / / /
Pseudomonas sp. 95 / / / / / / / / / 90 / / PO / 99 / / / / / 90 / /
R. pickettii 99 99 0 0 0 0 99 99 3 0 51 99 94 PO 0 99 4 88 33 0 28 99 0 /
R. radiobacter 99 99 99 99 99 99 99 99 0 / 0 99 34 PE 0 99 26 5 3 / / / / /
S. maltophilia 2 85 60 99 0 63 35 99 22 99 99 99 48 PO 2 99 22 0 93 99 0 29 0 /

183 Dried (Conventional) Neg panels - data tables: Dried Neg: Additional tests for the separation of gram-negative glucose nonfermenters/slow glucose fermenters
Table 11-D: Additional tests for the separation of gram-negative glucose nonfermenters/slow glucose fermenters (continued)
Organism Abbr. OXI OGL OLA OMA OMT OFS OXY 0%N 6%N DNA M36 CAT 42G FLG CET MAC SS N2 GEL 3FL ST TE LEC MEL
S. multivorum 99 99 99 99 0 99 99 99 25 0 0 99 0 N 0 99 0 0 0 / 58 84 0 /
S. paucimobilis 90 93 93 97 0 93 96 93 4 0 86 95 24 PO 0 10 0 0 2 99 50 98 0 /
S. putrefaciens 99 99 3 15 15 15 1 99 72 99 99 / 65 PO 0 99 72 0 97 0 2 89 74 /
S. spiritivorum 99 99 99 99 99 99 91 99 0 99 0 99 11 N 0 55 0 0 11 / 0 28 / /
Vibrio sp. SF 95 / / / / / / 0 90 / / / / / / / / / / / / / / /
W. virosa 99 0 0 0 0 0 0 99 7 4 0 98 70 N / 10 0 / 99 / 0 96 0 /
Y. pseudotb SF 0 / / / / / / 99 / 0 0 / / N / 99 / / 0 / / / / /

/ = Not Tested


185 Anaerobes - data table: Anaerobes: Additional identification tests for anaerobic nonspore-forming gram-negative bacilli
Anaerobes: Additional identification tests for anaerobic nonspore-forming gram-negative bacilli

Table 11-E: Additional identification tests for anaerobic nonspore-forming gram-negative bacilli
Organism KAN VAN COL BIL ESC CAT LIP CO2 GLU SUC LAC RHA SAL TRE ARA XLN XYL CEL PIG
B. fragilis R R R + + + - + + - - - - + V
B. vulgatus R R R + - - - + + + - - + + -
B. ovatus R R R + + - - + + + + + + + + +
B. thetaiota R R R + + + - + + + - + + - + +
B. uniformis R R R + + V - + + - + - + + +
B. eggerthii R R V + + - - + - + - - + + V
B. ureolyticus S R S - - - - - -
Capnocytophaga sp. MA + - - -
F. mortiferum S R S + + - - + -
F. necrophorum S R S V - + - - -
F. nucleatum S R S - - - - - -
F. varium S R S + - V - + -
P. bivia R R V - - - - + - + - - - -
P. buccae R R V - + - - + + + + + + +
P. corporis R R V - - - + - - - - +
P. disiens R R V - - - - + - - - - - -
P. distasonis R R R + + + - + + V + + V + +
P. melaninogen R R V - - - - + + + - - +
P. oralis grp. R R V - + - - + + + + - - +
P. asaccharolyt R S R - - - - - - - - +
P. gingivalis R S R - - - - - - - - +

186 Anaerobes - data table: Anaerobes: Additional identification tests for anaerobic nonspore-forming gram-negative bacilli
Legend
KAN Kanamycin 1.0mg LAC Lactose fermentation + Positive
VAN Vancomycin 5μg RHA Rhamnose fermentation - Negative
COL Colistin 10μg SAL Salicin fermentation V Variable
BIL Bile TRE Trehalose fermentation R Resistant
ESC Esculin ARA Arabinose fermentation S Susceptible
CAT Catalase XLN Xylan fermentation
LIP Lipase XYL Xylose fermentation
CO2 Growth in CO2 CEL Cellobiose fermentation
GLU Glucose fermentation PIG Pigment Production
SUC Sucrose fermentation MA Microaerophilic

187 Rapid Neg ID 4 panels - data table: Rapid Neg ID 4: Additional tests for the separation of gram-negative aerobic bacilli
Rapid Neg ID 4: Additional tests for the separation of gram-negative aerobic bacilli
% Positive Reactions* after 2.5 hours incubation at 35°C Legend: Numbers indicate percent positive reaction, / = not tested Tests 1-16
Table 11-F: Additional tests for the separation of gram-negative aerobic bacilli
Organism Abbr. OXI GLU OGL OLA OMA OMT OFS OXY 0%N 1%N 6%N 8%N 10N VNI GGL MR VP H2S CIT CUR ADO ARG DNA M36 SUC LAC MAL ARA CEL INO MLN MEL RAF RHA SOR TRE XYL NIT CAT 42G FLG CET MAC SS N2 GEL KCN 3FL IND
A. actinomycetem 19 99 / / / / / / 7 / 0 / / / 28 8 0 0 0 0 / / / 0 0 0 95 / / / / / / / / / 42 99 99 4 N / 5 0 0 / / / 0
A. caviae 99 99 / / / / / / 96 / / / / / 0 / 0 20 99 20 / 99 92 88 99 60 99 99 99 0 / 4 4 0 4 / / / 99 / PO / 80 / / 84 92 / 84
A. jandaei 99 99 / / / / / / 99 / / / / / 99 / 91 0 87 0 / 99 93 99 0 0 99 0 13 0 / 47 0 0 0 / / / 99 / PO / 80 / / 93 60 / 99
A. lwoffii 0 0 0 0 0 0 0 0 87 / 19 / / / / / / 0 33 26 / / / 0 / / / / / / / / / / / / / 6 99 48 N 0 78 / 0 2 / / 0
A. schubertii 99 99 / / / / / / 92 / / / / / 0 / 18 20 58 20 / 91 58 99 0 0 99 0 0 0 / 0 0 0 0 / / / 99 / PO / 80 / / 83 8 / 8
Ac baum/haem 0 0 99 75 54 0 0 98 99 / 13 / / / / / / 0 66 46 / / / 0 / / / / / / / / / / / / / 8 99 72 N 2 96 12 0 6 / / 0
Aer hyd/trot/ver 99 99 / / / / / / 99 / / / / / 84 / 57 0 84 59 / 99 90 99 72 14 98 32 50 0 / 1 1 8 0 / / / 98 / PO / 99 / / 98 49 / 98
Alsp/Ac.xyl/Rpau 99 0 25 0 0 0 0 35 99 / / / / / / / / 0 98 25 / / / 99 / / / / / / / / / / / / / 77 98 64 PE 40 99 76 / 0 / / 0
B. bronchisept 99 0 0 0 0 0 0 7 99 / 87 / / / / / / / 99 99 / 0 / 99 / / / / / / / / / / / / / 92 99 / PE 0 100 99 0 0 / / 0
B. cepacia cplx 99 0 99 96 93 99 53 93 99 / 7 / / / / / / 0 99 78 / 0 0 99 / / / / / / / / / / / / / 50 99 64 PO 66 91 24 0 45 / 99 0
B. diminuta 99 0 21 0 0 0 0 0 99 / 21 / / / / / / 34 1 13 / 0 14 99 / / / / / / / / / / / / / 3 98 38 PO 0 99 0 0 68 / 0 0
B. gladioli 47 / 99 37 0 99 0 99 99 / 10 / / / / / / 0 98 58 / 2 / / / / / / / / / / / / / / / 43 98 9 PO 4 99 4 0 12 / / 0
B. pseudomallei 99 0 99 99 99 99 70 99 99 / 12 / / / / / / 0 81 21 / 99 / 99 / / / / / / / / / / / / / 99 99 99 PE 10 99 39 99 79 / / 0
B. vesicularis 98 0 87 0 94 0 0 27 95 / 23 / / / / / / 49 1 2 / 0 0 99 / / / / / / / / / / / / / 5 / 19 PO 0 43 1 0 25 / 0 0
B. zoohelcum 99 0 0 0 0 0 0 0 15 / 0 / / / / / / 0 0 99 / 99 0 0 / / / / / / / / / / / / / 0 99 10 N / 2 0 0 98 / / 98
C. amalonaticus 0 99 / / / / / / 99 / / / / / 97 99 0 5 95 85 0 85 0 95 9 35 99 99 99 0 1 0 5 99 99 99 99 99 / / PE / 99 / / 0 99 / 99
C. farmeri 0 99 / / / / / / 99 / / / / / 96 99 0 0 10 59 0 85 0 97 99 15 99 99 99 0 0 99 99 99 98 99 99 99 / / PE / 99 / / 0 93 / 99
C. freundii cplx 0 99 / / / / / / 99 / / / / / 95 99 0 55 70 67 / 60 / 80 23 67 99 99 66 0 / 49 12 99 99 99 99 98 / / / / / / / 0 85 / 33
C. indologenes 96 0 98 0 98 10 14 31 99 / 0 / / / / / / 1 3 42 / / 4 0 / / / / / / / / / / / / / 22 99 42 N / 63 0 0 78 / / 98
C. koseri 0 99 / / / / / / 99 / / / / / 98 99 0 0 99 75 99 80 0 95 40 50 99 99 99 0 95 0 0 99 99 99 99 99 / / PE / 99 / / 0 0 / 99
C. meningosepti 99 0 95 57 99 99 0 3 99 / 7 / / / / / / 3 12 8 / 33 99 0 / / / / / / / / / / / / / 0 99 45 N / 92 1 0 91 / / 99
C. sakazakii 0 99 / / / / / / 99 / / / / / 98 59 9 0 99 1 0 99 0 96 99 99 99 99 99 75 18 99 99 99 0 99 99 99 / / PE / 99 / / 0 99 / 11
C. violaceum 67 99 / / / / / / 99 / 8 / / / 0 / / / 68 5 / 99 / 99 26 0 3 / / / / / / / / / 0 97 97 / PO / 99 / / 86 / / 21
CDC group EO-2 99 0 99 99 9 0 0 99 64 / 36 / / / / / / 0 99 91 / 0 / 0 / / / / / / / / / / / / / 99 82 36 N 0 82 0 18 0 / / 0
Cedecea davisae 0 99 / / / / / / 99 / / / / / 70 99 50 0 95 0 0 50 0 95 99 19 99 0 99 0 91 0 10 0 0 99 99 99 / / PE / 99 / / 0 86 / 0
Cedecea lapagei 0 99 / / / / / / 99 / / / / / 99 40 80 0 99 0 0 80 0 80 0 60 99 0 99 0 99 0 0 0 0 99 0 99 / / PE / 99 / / 0 99 / 0
Cedecea neteri 0 99 / / / / / / 99 / / / / / 99 99 50 0 99 0 0 99 0 99 99 35 99 0 99 0 99 0 0 0 99 99 99 99 / / PE / 99 / / 0 65 / 0
Cedecea sp. 3/5 0 99 / / / / / / 99 / / / / / 99 99 50 0 99 0 0 75 0 99 75 0 99 0 99 0 0 99 99 0 50 99 99 99 / / PE / 99 / / 0 99 0
D. acido/C. test 99 0 0 0 0 50 0 0 99 / 3 / / / / / / 28 70 4 / 0 1 99 / / / / / / / / / / / / / 97 / / PO 2 99 / 0 6 / 99 0
E. aerogenes 0 99 / / / / / / 99 / / / / / 99 5 98 0 95 2 98 0 0 97 99 95 99 99 99 95 95 99 96 99 99 99 99 99 / / PE / 99 / / 0 98 / 0

Table 11-F: Additional tests for the separation of gram-negative aerobic bacilli (continued)
E. albertii 0 99 / / / / / / / / / / / / 99 / 0 0 0 0 0 0 0 0 0 0 60 99 0 0 / 0 0 0 0 60 0 99 99 / / / / / / 0 0 / 0
E. americana 0 99 / / / / / / 99 / / / / / 0 84 95 0 95 0 0 0 0 60 0 70 16 0 10 0 0 0 0 23 0 99 13 97 / / PE /99 / / 0 5 / 0 0
E. amnigenus 1 0 99 / / / / / / 99 / / / / / 99 7 99 0 70 0 0 9 0 92 99 70 99 99 99 0 91 99 99 99 9 99 99 99 / / PE / 99 / / 0 99 / 0
E. amnigenus 2 0 99 / / / / / / 99 / / / / / 99 65 99 0 99 0 0 35 0 99 0 35 99 99 99 0 99 99 0 99 99 99 99 99 / / PE / 99 / / 0 99 / 0
E. asburiae 0 99 / / / / / / 99 / / / / / 95 99 2 0 99 60 0 21 0 0 99 75 99 99 99 0 3 0 70 5 99 99 97 99 / / N / 99 / / 0 97 / 0
E. brevis 99 0 99 0 99 0 0 0 99 / / / / / / / / 0 0 0 / 0 / 0 / / / / / / / / / / / / / 0 99 0 N / 99 0 0 99 / / 99
E. cancerogenus 0 99 / / / / / / 99 / / / / / 99 5 99 0 99 1 0 94 0 99 0 10 99 99 99 0 99 0 0 99 1 99 99 99 / / PE / 99 / / 0 98 / 0
E. cloacae 0 99 / / / / / / 99 / / / / / 99 59 9 0 99 65 25 97 0 95 97 93 99 99 99 15 75 90 97 92 95 99 99 99 / / PE / 99 / / 0 98 / 0
E. coli 0 99 / / / / / / 99 / / / / / 95 99 0 1 1 1 5 17 0 95 50 95 95 99 2 1 0 75 50 80 94 98 95 99 / / PE / 99 / / 0 3 / 98
E. coli-0157:H7 0 99 / / / / / / 99 / / / / / 90 99 0 1 1 1 5 17 0 95 50 95 95 99 2 1 0 75 50 80 1 98 95 99 / / PE / 99 / / 0 3 / 98
E. corrodens 99 0 0 0 0 0 0 0 71 / / / / / / / / 0 0 0 / / / 0 / / / / / / / / / / / / / 99 8 / N 0 0 0 0 0 / / 0
E. fergusonii 0 99 / / / / / / 99 / / / / / 95 99 0 0 17 0 98 5 0 93 0 0 96 98 96 0 35 0 0 92 0 96 96 99 / / PE / 99 / / 0 0 / 98
E. gergoviae 0 99 / / / / / / 99 / / / / / 98 5 99 0 99 93 0 0 0 90 98 55 99 99 99 0 96 97 97 98 0 99 99 99 / / PE / 99 / / 0 0 / 0
E. hermannii 0 99 / / / / / / 99 / / / / / 97 99 0 0 1 0 0 0 0 99 45 45 99 99 97 0 0 0 40 97 0 99 99 99 / / PE / 99 / / 0 94 / 99
E. hormaechei 0 99 / / / / / / 99 / / / / / 83 57 99 0 96 87 0 78 0 52 99 99 99 99 9 0 99 0 0 99 0 99 96 99 / / PE / 99 / / 0 99 / 0
E. tarda 0 99 / / / / / / 99 / / / / / 99 99 0 99 1 0 0 0 0 98 0 0 99 9 0 0 0 0 0 0 0 0 0 99 / / PE / 99 / / 0 0 / 99
E. vulneris 0 99 / / / / / / 99 / / / / / 97 99 0 0 0 0 0 30 0 99 8 15 99 99 99 0 85 99 99 93 1 99 99 99 / / PE / 99 / / 0 15 / 0
G. hollisae 99 99 / / / / / / 0 99 83 0 0 99 0 / / 0 / / / / / / / / / / / / / / / / / / / / / / N / / / / / / / 97
Hafnia alvei 0 99 / / / / / / 99 / / / / / 98 40 85 0 10 4 0 6 0 85 10 59 99 51 5 0 50 0 2 97 0 95 98 99 / / PE / 99 / / 0 95 / 0
K. intermedia 0 99 / / / / / / 99 / / / / / 99 99 99 0 65 0 0 0 0 89 65 99 99 99 99 0 99 99 99 99 99 99 99 99 / / PE / 99 / / 0 65 / 0
K. oxytoca 0 99 / / / / / / 99 / / / / / 97 20 95 0 95 90 99 0 0 0 99 99 99 98 99 98 98 99 99 99 99 99 99 99 / / N / 99 / / 0 97 / 99
K. ozaenae 0 99 / / / / / / 99 / / / / / 50 98 0 0 30 10 97 6 0 0 20 30 95 98 92 55 3 97 90 55 65 98 95 80 / / N / 99 / / 0 88 / 0
K. pneumoniae 0 99 / / / / / / 99 / / / / / 97 10 98 0 98 95 90 0 0 0 99 98 98 99 98 95 93 99 99 99 99 99 99 99 / / N / 99 / / 0 98 / 0
K. rhinoscler 0 99 / / / / / / 99 / / / / / 0 99 0 0 0 0 99 0 0 0 75 0 99 99 99 95 95 99 90 96 99 99 99 99 / / N / 99 / / 0 80 / 0
Kingella sp. 99 93 / / / / / / 71 / 4 / / / / / / 0 0 0 / / / / 0 0 50 / / / / / / / / / 0 48 5 29 / / 5 0 44 1 / / 0
Kluyvera sp. 0 99 / / / / / / 99 / / / / / 94 99 0 0 88 0 0 0 0 94 90 97 99 99 99 0 91 99 99 99 43 99 95 99 / / PE / 99 / / 0 89 / 91
L. adecarboxy 0 99 / / / / / / 99 / / / / / 97 99 0 0 0 48 93 0 0 79 66 93 99 99 99 0 93 99 66 99 0 99 99 99 / / PE / 99 / / 0 97 / 99
Leminorella sp. 0 99 / / / / / / 99 / / / / / 17 50 0 99 50 0 0 0 0 0 0 0 0 99 0 0 0 0 0 0 0 0 92 99 / / N / / / / 0 0 / 0
M. morganii 0 99 / / / / / / 99 / / / / / 90 95 0 20 0 95 0 0 0 95 0 1 0 0 0 0 1 0 0 0 0 0 0 90 / / PE / 99 / / 0 98 / 95
M. wisconsensis 0 99 / / / / / / 99 / / / / / 0 99 0 0 80 0 99 0 0 0 99 99 30 0 0 0 0 99 99 0 0 0 0 90 / / N / 99 / / 0 70 / 0
Morax/Psychr spp. 99 / 0 0 0 0 0 0 58 / 10 / / / / / / 0 0 0 / / / 0 / / / / / / / / / / / / / / 91 32 N / 55 9 / / / / 0
Myroides sp. 99 0 0 0 0 0 0 0 99 / 25 / / / / / / 0 0 99 / 99 / 0 / / / / / / / / / / / / / 0 99 31 N 0 96 41 0 96 / / 0
N elo/weav/zoo 99 0 33 0 0 0 0 0 90 / 5 / / / / / / 0 7 0 / / / 0 / / / / / / / / / / / / / 98 67 44 N / 57 0 60 3 / / 0

O. anthropi 99 0 99 0 64 57 50 99 99 / 60 / / / / / / 43 64 99 / 71 / 99 / / / / / / / / / / / / / 86 99 64 PE 3 99 99 43 0 / / 0
O. ureolytica 99 0 0 0 0 0 0 0 22 / 20 / / / / / / 0 30 97 / / 0 99 / / / / / / / / / / / / / 99 99 18 PE 0 89 56 0 0 / / 0
O. urethralis 99 0 0 0 0 0 0 0 96 / 59 / / / / / / 0 46 0 / / 0 0 / / / / / / / / / / / / / 0 99 59 N / 96 9 0 0 / / 99
P. aerogenes 99 99 / / / / / / 99 / / / / / 99 / / / 0 99 / 0 / 0 94 57 99 / / / / / / / / / 81 99 99 / N / 99 6 0 0 / / 0
P. aeruginosa 99 0 87 0 17 0 0 90 99 / 65 / / / / / / 0 / 57 / 99 10 97 / / / / / / / / / / / / / 98 / 99 PO 94 99 96 93 82 / 0 0
P. agglomerans 0 99 / / / / / / 99 / / / / / 20 50 70 0 50 20 7 0 0 85 75 40 89 95 55 15 65 50 30 85 30 97 93 85 / / PE / 99 / / 23 5 / 20
P. alcalifaciens 0 99 / / / / / / 99 / / / / / 85 99 0 0 98 0 98 0 0 96 15 0 1 1 0 1 0 0 1 0 1 2 1 99 / / PE / 99 / / 0 99 / 99
P. damsela 95 99 / / / / / / 0 99 95 0 0 99 10 / / 0 / / / / / / / / / / / / / / / / / / / / / / N / / / / / / / 0
P. luminescens 0 99 / / / / / / / / / / / / 0 0 0 0 20 99 0 0 0 99 0 0 0 0 0 0 / 0 0 0 0 0 0 0 / / / / 99 / / 99 60 / 0
P. luteola 0 0 99 27 99 94 12 99 99 / 74 / / / / / / / / 64 / 99 0 99 / / / / / / / / / / / / / 62 / 94 PO 0 99 68 0 61 / / 0
P. mendocina 99 / 99 0 0 0 0 99 99 / 99 / / / / / / 0 99 50 / 99 / 99 / / / / / / / / / / / / / 99 99 99 PO 99 99 99 99 0 / 0 0
P. mirabilis 0 99 / / / / / / 99 / 87 / / / 96 97 50 98 65 98 0 0 50 95 15 2 0 0 1 0 2 0 1 1 0 98 98 95 / / PE / 99 / / 90 98 / 2
P. multocida 96 99 / / / / / / 80 / / / / / 0 / / / 0 0 / 3 / 0 99 8 1 / / / / / / / / / 85 99 98 / N / 2 0 0 0 / / 98
P. oryzihabitans 0 0 99 36 97 99 25 99 99 / 62 / / / / / / / / 77 / 14 0 99 / / / / / / / / / / / / / 6 / 33 PO 53 99 22 0 17 / / 0
P. rettgeri 0 99 / / / / / / 99 / / / / / 10 93 0 0 95 98 99 0 0 94 15 5 2 0 39 0 0 5 57 0 1 0 10 99 / / PE / 99 / / 0 97 / 99
P. rustigianii 0 99 / / / / / / 99 / / / / / 35 65 0 0 15 0 0 0 0 30 35 0 0 0 0 0 0 0 0 0 0 0 0 99 / / PE / 99 / / 0 99 / 98
P. shigelloides 99 99 / / / / / / 99 / / / / / 0 90 0 0 0 0 0 98 0 95 0 80 95 0 0 95 0 70 0 0 0 99 0 99 93 / PO 0 99 96 0 0 1 / 99
P. stuartii 0 99 / / / / / / 99 / / / / / 0 99 0 0 93 30 5 0 10 85 50 2 1 1 5 95 0 0 7 0 1 98 7 99 / / PE / 99 / / 0 99 / 98
P. stutzeri 99 / 99 0 99 93 0 99 96 / 96 / / / / / / 0 96 55 / 0 / 99 / / / / / / / / / / / / / 99 99 69 PO 4 99 54 99 0 / 0 0
P. yeei 99 0 99 99 9 0 0 99 64 / 36 / / / / / / 0 99 91 / 0 / 0 / / / / / / / / / / / / / 99 82 36 N 0 82 0 18 0 / / 0
(CDC EO-2)
Ppneu/Aure/ 98 99 / / / / / / 59 / / / / / 0 / / / 0 65 / 0 / 0 99 34 98 / / / / / / / / / 65 99 86 / N / 44 / 0 0 / / 30
Mhaem
Prt vulg/penneri 0 99 / / / / / / 99 / / / / / 70 98 25 62 32 98 0 0 45 90 57 15 0 0 1 0 1 0 1 1 0 76 98 92 / / PE / 99 / / 70 98 / 49
Ps alcal/psalc 98 0 5 0 0 0 0 15 99 / 55 / / / / / / 0 / 5 / 45 0 99 / / / / / / / / / / / / / / / 47 PO / 98 / / 0 / 0 0
Ps fluor/putida 99 0 99 31 16 39 / 99 99 / 71 / / / / / / 0 96 63 / 99 0 99 / / / / / / / / / / / / / 10 / 0 PO 88 99 97 1 / / / 0
R. ornithinolytica 0 99 / / / / / / 99 / / / / / 99 96 70 0 99 99 99 0 0 0 99 99 99 99 99 95 99 99 99 99 99 99 99 99 / / N / 99 / / 0 99 / 99
R. pickettii 99 0 99 0 0 0 0 99 99 / 3 / / / / / / 0 / 99 / 0 0 99 / / / / / / / / / / / / / 99 / 94 PO 0 77 0 99 33 / 0 0
R. radiobacter 99 0 99 99 99 99 99 99 99 / 18 / / / / / / 14 99 97 / 8 / 99 / / / / / / / / / / / / / 83 98 34 PE 0 99 25 5 2 / / 0
Roseomonas sp. 92 0 10 0 0 9 0 64 99 / 13 / / / 3 / / 0 69 99 / / / 67 / / / / / / / / / / / / / 51 99 89 / / 99 3 3 / / / 0
S. ficaria 8 99 / / / / / / 99 / / / / / 0 75 75 0 99 0 0 0 99 99 99 15 99 99 99 55 0 40 70 35 99 99 99 92 / / PE / 99 / / 99 55 / 0
S. fonticola 0 99 / / / / / / 99 / / / / / 79 99 9 0 91 13 99 0 0 91 21 97 97 99 63 0 88 98 99 76 99 99 85 99 / / PE / 99 / / 0 70 / 0
S. liquefac cplx 0 99 / / / / / / 99 / / / / / 75 93 93 0 90 3 5 0 85 95 98 10 98 98 56 0 2 75 85 15 95 99 99 99 / / PE / 99 / / 90 90 / 1
S. maltophilia 0 0 85 60 99 0 63 35 99 / / / / / / / / 95 34 3 / 0 99 99 / / / / / / / / / / / / / 39 99 48 PO 29 92 2 0 93 / 99 0

S. marcescens 0 99 / / / / / / 99 / / / / / 55 20 98 0 98 15 40 0 98 97 99 2 96 0 5 75 3 0 2 0 99 99 7 98 / / PE / 99 / / 90 95 / 1
S. multivorum 99 / 99 99 99 0 99 99 99 / 25 / / / / / / 0 0 95 / 0 / 0 / / / / / / / / / / / / / 0 99 0 / 0 99 0 0 0 / / 0
S. odorifera 1 0 99 / / / / / / 99 / / / / / 0 99 50 0 99 55 0 0 99 99 99 70 99 99 99 99 0 98 99 95 99 99 99 99 / / PE / 99 / / 95 60 / 60
S. odorifera 2 0 99 / / / / / / 99 / / / / / 13 60 99 0 97 0 55 0 99 99 0 97 99 99 99 99 0 96 7 94 99 99 99 99 / / PE / 99 / / 94 19 / 50
S. Paratyphi A 0 99 / / / / / / 99 / / / / / 99 99 0 10 0 0 0 15 0 95 0 0 95 99 5 0 0 95 0 99 95 99 0 99 / / PE / 99 99 / 0 0 / 0
S. paucimobilis 90 0 99 99 99 0 99 99 99 / 0 / / / / / / 0 0 0 / 8 0 99 / / / / / / / / / / / / / 0 99 24 PO 0 0 0 0 0 / 0 0
S. plymuthica 0 99 / / / / / / 99 / / / / / 40 94 80 0 75 0 0 0 99 50 99 80 94 99 88 50 0 93 94 0 65 99 94 99 / / N / 99 / / 60 30 / 0
S. putrefaciens 99 0 50 0 99 0 99 0 99 / 69 / / / / / / 96 8 12 / 0 99 99 / / / / / / / / / / / / / 99 99 30 PO 49 9 8 0 65 / 0 0
S. rubidaea 0 99 / / / / / / 99 / / / / / 30 20 99 0 95 2 99 0 99 85 98 99 99 99 94 20 94 99 99 1 1 99 99 99 / / PE / 99 / / 90 25 / 0
S. spiritivorum 99 / 99 99 99 99 99 99 99 / 0 / / / / / / 0 0 99 / 25 / 0 / / / / / / / / / / / / / 0 99 9 / 0 46 0 0 15 / / 0
S. Typhi 0 99 / / / / / / 99 / / / / / 0 99 0 97 0 0 0 3 0 97 0 1 97 2 0 0 0 99 2 0 99 99 82 99 / / PE / 99 99 / 0 0 / 0
S. entérica 0 99 / / / / / / 99 / / / / / 96 99 0 95 95 1 0 70 2 95 1 1 / 99 53 5 0 95 2 95 / 99 97 99 / / PE / 99 99 / 0 0 / 1
Shigella sp. 0 99 / / / / / / 99 / / / / / 1 99 0 0 0 0 0 7 0 0 1 1 39 74 1 0 0 24 11 28 26 85 5 99 / / / / 99 99 / 0 0 / 30
Tatumella 0 99 / / / / / / 99 / / / / / 0 0 5 0 2 0 0 0 0 0 98 0 0 0 0 0 0 25 11 0 0 93 9 98 / / PO / 99 / / 0 0 / 0
V. cholerae 99 99 / / / / / / 99 99 53 1 0 99 0 99 75 0 97 0 0 0 93 99 99 79 9 0 8 0 1 1 0 0 1 99 0 99 / / N / / / / 90 10 / 99
V. metschnikovi 0 99 / / / / / / 0 99 78 44 4 0 0 / / 0 / / / / / / / / / / / / / / / / / / / / / / N / / / / / / / 20
V. mimicus 99 99 / / / / / / 99 99 49 0 0 99 0 99 9 0 99 1 0 0 55 98 0 21 99 1 0 0 0 0 0 0 0 94 0 / / / N / / / / 60 2 / 98
V. para/algino 99 99 / / / / / / 0 99 99 87 36 99 0 / / 0 / / / / / / / / / / / / / / / / / / / / / / N / / / / / / / 82
V.vulnificus 99 99 / / / / / / 0 99 65 0 0 99 0 / / 0 / / / / / / / / / / / / / / / / / / / / / / N / / / / / / / 97
Vib fluv/furn 99 99 / / / / / / 0 99 98 75 2 99 / / / 0 / / / / / / / / / / / / / / / / / / / / / / N / / / / / / / 12
W. virosa 98 0 0 0 0 0 0 0 99 / 7 / / / / / / 0 0 0 / 0 3 0 / / / / / / / / / / / / / 0 98 70 N / 10 0 / 99 / / 99
Y. entero group 0 99 / / / / / / 90 / / / / / 22 97 2 0 5 76 0 0 1 4 73 22 93 93 92 20 1 20 20 50 99 99 88 98 / / N / 99 / / 0 3 / 70
Y. pestis 0 99 / / / / / / / / / / / / 0 80 0 0 0 5 0 0 0 0 0 0 80 99 0 0 0 20 0 15 0 99 90 85 / / / / 50 / / 0 0 / 0
Y. pseudotb 0 99 / / / / / / 99 / / / / / 0 99 0 0 0 95 0 0 0 0 0 0 95 50 0 0 0 70 15 70 0 99 99 95 / / N / 99 / / 0 0 / 0
Y. regensburgei 0 99 / / / / / / 99 / / / / / 99 99 0 0 92 0 0 8 0 99 0 0 99 99 99 0 0 92 25 99 0 99 99 99 99 / PE / 99 / / 0 92 / 0
Y. ruckeri 0 99 / / / / / / 99 / / / / / 59 71 0 0 0 0 0 5 0 0 0 0 95 5 5 0 0 0 5 0 50 95 0 75 / / N / 99 / / 30 15 / 0

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MICROBIOLOGY

PLURONIC® Surfactants is a registered trademark of BASF Corporation, Parsippany, NJ USA

Limitations and conditions

Document Date Revisions

© 2015 Beckman Coulter, Inc. All rights reserved. Spec: 9900-3929

1 Rapid Neg ID 3 panels: Gram-negative aerobic bacilli . . . . . . . . . . . . . . . . . . . . . . . . . .11

2 Dried (Conventional) Pos panels: Gram-positive aerobic bacteria . . . . . . . . . . . . . . . .33

MicroScan Microbiologics Information Manual 9020-7751 Rev. A

3 Dried (Conventional) Neg panels: Gram-negative aerobic bacilli . . . . . . . . . . . . . . . . .57

4 Yeast and yeast-like microorganisms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .79

9020-7751 Rev. A MicroScan Microbiologics Information Manual

Table 4-C: Typical characteristics by species . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83

6 Haemophilus - Neisseria . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .103

MicroScan Microbiologics Information Manual 9020-7751 Rev. A

Table 6-C: Tests included on HNID panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 108

7 Vancomycin and Streptomycin Synergy Screen Susceptibility algorithm . . . . . . . . .111

8 Panel Processing (Read Times) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .113

9 Rapid Neg ID 4 panels: Gram-negative aerobic bacilli . . . . . . . . . . . . . . . . . . . . . . . . .119

9020-7751 Rev. A MicroScan Microbiologics Information Manual

Additional tests . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 139

10 Rapid Pos ID 2 panels: Gram-positive aerobic bacteria . . . . . . . . . . . . . . . . . . . . . . .143

MicroScan Microbiologics Information Manual 9020-7751 Rev. A

Printing the large format data tables . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 174

9020-7751 Rev. A MicroScan Microbiologics Information Manual

This section includes the following topic:

• Introduction (page 10)

MicroScan Microbiologics Information Manual 9020-7751 Rev. A

Who should use this guide

• Rapid Neg ID 3 panels: Gram-negative aerobic bacilli (page 11)

• Dried (Conventional) Pos panels: Gram-positive aerobic bacteria (page 33)

• Dried (Conventional) Neg panels: Gram-negative aerobic bacilli (page 57)

• Yeast and yeast-like microorganisms (page 79)

• Anaerobes (page 93)

• Haemophilus - Neisseria (page 103)

• Rapid Neg ID 4 panels: Gram-negative aerobic bacilli (page 119)

• Rapid Pos ID 2 panels: Gram-positive aerobic bacteria (page 143)

9020-7751 Rev. A MicroScan Microbiologics Information Manual

This section includes the following topics:

• Taxa in database (page 12)

• Supplemental information (page 22)

• Special characteristics (page 22)

• Footnotes (page 23)

• Additional tests (page 28)

• Tests included on Rapid Neg panels (page 30)

MicroScan Microbiologics Information Manual 9020-7751 Rev. A

• Taxa for gram-negative fermenters (in this section)

• Taxa for gram-negative nonfermenters (page 19)

Taxa for gram-negative fermenters

Aer hyd/trot/ver Aeromonas hydrophila complex N/A

Aeromonas trota N/A

A. jandaei Aeromonas jandaei N/A

A. schubertii Aeromonas schubertii N/A

Aeromonas sp. Aeromonas species N/A

Aeromonas caviae complex N/A

Aeromonas hydrophila complex N/A

Aeromonas jandaei N/A

Aeromonas schubertii N/A

Aeromonas trota N/A

Aeromonas veronii biovar veronii Aeromonas veronii

Aeromonas veronii biovar sobria Aeromonas sobria

Cedecea davisae Cedecea davisae N/A

Cedecea lapagei Cedecea lapagei N/A

Cedecea neteri Cedecea neteri N/A