INTISARI

CAHYANTO, D.M., 2016, PENGARUH PEMBERIAN MINYAK IKAN NILA

(Oreochromis nilotikus) TERHADAP KADAR HDL DAN LDL PADA TIKUS
HIPERLIPIDEMIA, SKRIPSI, FAKULTAS FARMASI, UNIVERSITAS SETIA
BUDI, SURAKARTA.

Hiperkolesterolemia merupakan keadaan dimana terjadi peningkatan kadar

kolesterol di dalam darah, meningkatnya kadar kolesterol akan disertai dengan
peningkatan kadar LDL dalam darah yang dapat memicu terjadinya berbagai penyakit
seperti jantung koroner dan stroke. Minyak ikan dikenal memiliki banyak manfaat
karena kandungan asam lemak tak jenuhnya, diantaranya dalam menurunkan
kolesterol. Tujuan dari penelitian ini untuk mengetahui pengaruh minyak ikan nila
(Oreochromis nilotikus) terhadap kadar LDL dan HDL serta dosis efektif dalam
menurunkan kadar LDL dan meningkatkan kadar HDL pada tikus hiperlipidemia.
Tiga puluh ekor tikus dibagi menjadi 6 kelompok. Kelompok 1 (kontrol
normal) tanpa perlakuan, kelompok II (kontrol negatif) diberi PGA 0.5%, kelompok
III (kontrol positif) diberi dosis simvastatin 0,36 mg/200gBB, kelompok IV, V dan VI
diberi dosis perlakuan miyak ikan 0.8 ml ; 1.6 ml ; dan 3.2 ml/200gBB. Tikus diberi
perlakuan selama 21 hari dengan rentang waktu hari ke-0 sebelum perlakuan, hari ke-
14 setelah pemberian diet tinggi lemak dan hari ke-21 setelah pemberian dosis minyak
ikan, sampel darah diambil melalui vena mata, kadar LDL dan HDL diuji dengan
ANOVA dengan uji lanjutan Post Hoc test.
Hasil penelitian menunjukkan pemberian minyak ikan nila pada dosis 3.2
ml/200gBB berpengaruh terhadap kadar HDL dan LDL pada tikus hiperlipidemia
tetapi kurang efektif jika dibandingkan dengan konterol positif simvastatin di mana
persentase peningkatan dan penurunannya tidak lebih dari 50%.

Kata kunci : hiperkolesterolemia, Oreochromis nilotikus, kadar LDL dan HDL, minyak
ikan.
 ABSTRAK
CAHYANTO, D.M., 2016, THE EFFECT OF TILAPIA FISH OIL (Oreochromis
nilotikus) ON HDL AND LDL LEVELS IN MOUSE HYPERLIPIDEMIA, THESIS,
FACULTY OF PHARMACY, SETIA BUDI UNIVERSITY, SURAKARTA.

Hypercholesterolemia is a condition where an increase in cholesterol levels in

the blood, increased cholesterol levels will be accompanied by elevated levels of LDL
in the blood which can lead to various diseases such as coronary heart disease and
stroke. Fish oil is known to have many benefits due to the content of unsaturated fatty
acids, including cholesterol-lowering. The purpose of this study was to determine the
effect of tilapia fish oil (Oreochromis nilotikus) on levels of LDL and HDL and also
the dose is effective in lowering LDL levels and increasing HDL levels in mice with
hyperlipidemia.
Thirty mice were divided into 6 groups. Group 1 (normal control) without
treatment, group II (negative control) were given PGA 0.5%, Group III (positive
control) were given doses of simvastatin 0,36/200gBW, group IV, V and VI were given
a dose of 0.8 ml fish oil treatment; 1.6 ml ; and 3.2 ml / 200gBW. Mice were treated
for 21 days with a span of days 0 before treatment, the 14th day after the administration
of high-fat diet and the 21st day after the administration of doses of fish oil, blood
samples were taken through a vein eyes, levels of LDL and HDL were tested by
ANOVA continued Post Hoc test.
The results showed tilapia fish oil at dose of 3.2 ml / 200gBW effected on HDL
and LDL cholesterol levels in rats with hyperlipidemia but was less effective if
compared with simvastatin positive control where the percentage increasing or decline
was no more than 50%.

Keywords: hypercholesterolemia, Oreochromis niloticus, LDL and HDL levels, fish

oil