Medical
Microbiology
(Including Parasitology)
The Short Textbook of
Medical
Microbiology
(Including Parasitology)
Tenth Edition
Satish Gupte MD
Professor and Head
Department of Microbiology
Gian Sagar Medical College and Hospital
Ramnagar (Banur), Distt. Patiala. (Punjab)
Ex-Professor and Head
Department of Microbiology
Adesh Institute of Medical Sciences and Research Centre
Bathinda (Punjab)
and
Govt. Medical College and Associated Hospitals
Jammu 180001, J & K, India
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The Short Textbook of Medical Microbiology (Including Parasitology)
© 2010, Mrs Jyotsna Gupte
All rights reserved. No part of this publication should be reproduced, stored in a retrieval system, or transmitted
in any form or by any means: electronic, mechanical, photocopying, recording, or otherwise, without the prior
written permission of the author and the publisher.
This book has been published in good faith that the material provided by author is original. Every effort is
made to ensure accuracy of material, but the publisher, printer and author will not be held responsible for
any inadvertent error(s). In case of any dispute, all legal matters are to be settled under Delhi jurisdiction
only.
Foreword
Dr Satish Gupte has done wonderful job in updating his book to tenth edition. The
simplicity of language and scholarly work done in the book shows the effort of author
for updating the book.
I wish more undergraduate and postgraduate students will read this book for
updating their knowledge and skills.
Dr AS Sekhon MD
Principal
Gian Sagar Medical College and Hospital
Ram Nagar (Banur)
Distt. Patiala, Punjab
India
Preface to the Tenth Edition
Over 27 years back first edition of The Short Textbook of Medical Microbiology was brought out in 1982.
Since then the volume is galloping while passing through silver jubilee year landing right into revised,
enlarged and updated tenth edition decorated elegantly and colorfully with entirely fresh and new get
up. Indonesian edition of this volume was published in 1990 with collaboration of Binarupa Aksara
under the title MICROBIOLOGI DASAR earning fame and name in that region.
It has been kept in mind to condense and include the latest major meaningful advances in medical
microbiology in this volume. Distinct feature of this volume is inclusion of updated Medical Parasitology
with Entomology. All the chapters are thoroughly revised and updated as highlighted by esteemed
readers from time to time. New chapters are included namely; microbiology in the service of human
being, molecular techniques in microbiology, emerging and re-emerging infections and laboratory and
hospital wastes. Also included in this volume: Biofilm, new methods of disinfection and sterilization,
super antigen, Ogawa medium, adenosine deaminase activity, immunological investigations of
Mycobacterium tuberculosis, Napah virus, pandemic of swine flu (H1N1) breakthrough 2009, laboratory
diagnosis of septicemia/bacteremia and infective endocarditis and Nobel prize in Medicine etc.
The challenge was to keep the volume comprehensive, profusely colored, beautifully illustrated and
still handy maintaining emphasis on the needs of MBBS and BDS students. Several colored figures and
photographs, tables and colored illustrations (adjusted appropriately between texts) are included to
enhance and intensify the utility of this volume. The chapters pertaining to clinical microbiology are
especially addressed to budding doctors (interns and house surgeons/physicians) who usually face
problems in collection and transportation of clinical samples for laboratory investigations.
It is the privilege to offer my sincere gratitude to my respected teachers: Professor TD Chugh, Professor
Uma Sabharwal, Professor RK Arya and Professor DR Arora for their blessings, encouragement, inspiration
and good will. My special thanks to Professor Aruna Aggarwal, Professor BL Sherwal, Professor Jagdish
Chander, Professor Prem Narwan, Professor Raj Kumar, Professor Anjum, Professor Bella Mahajan and
many others for their best wishes and valuable suggestions in upbringing the current edition of this
volume.
I am thankful to Dr GM Warke of Hi Media Laboratory, Mumbai, for kind permission to use some
photographs.
I acknowledge the help rendered by faculty of Department of Microbiology, Gian Sagar Medical
College and Hospital, (Banur), Patiala in the preparation of this edition. Thanks to Mr Gurpreet Singh for
taking responsibility of secretarial work of this volume.
Professor AS Sekhon, Dean and Principal, Gian Sagar Medical College, (Banur), Patiala has encouraged
me and enriched me with his good wishes to accomplish the commitment of bringing up this edition to
the contentment and expectations of readers. From the core of my heart, I am grateful to him indeed.
My sincere thanks to Shri Jitendar P Vij (Chairman and Managing Director), Jaypee Brothers Medical
Publishers (P) Ltd., New Delhi and his dedicated and dynamic team for publishing this book elegantly
and speedily.
In true sense I derived much inspiration and blessings from my mother, respected Late Smt. Kaushalya
Gupte and respected father Late Shri Charanjit Gupte. Also, I take opportunity to express my thanks to
all my family members especially to my wife Mrs Jyotsna Gupte and dear son Anubhav Gupte who
actually helped me sincerely while I was simply engrossed in preparing and also during finalizing this
manuscript.
Comments, suggestions and constructive criticisms towards improvements of future editions of this
volume are very much welcomed. In fact, they underline the shortcomings which are looked into and
rectified in the interest of our esteemed readers.
Part I
General Bacteriology
Part II
Bacterial Genetics
Part III
Immunology
Part IV
Systematic Bacteriology
Part V
Virology
Part VI
Mycology
55. Mycology ............................................................................................................................. 331
Part VII
Parasitology
Part VIII
Clinical Microbiology
Part IX
Appendices