Anda di halaman 1dari 13

Name: Revina J.

Borgoños Section: Pharmacy 2B Date Performed: July 8, 2019

Instructor: Jonah Micah J. Madera Date Submitted: July 15, 2019

Experiment 2

Colloids

I. OBJECTIVES:

1. Prepare the two types of colloidal systems.

2. Differentiate between emulsoid and suspensoid in terms of properties and reactions.

II. PROCEDURE:

A. Preparation of Emulsoid

 10 grams of gelatin was dissolved in a beaker containing a 200 ml of hot water

with a constantly stirring.

 1 ml of the prepared emulsoid was poured in a test tube and cooled down under a

tap water.

 The test tube containing emulsoid was brought and heated again using a water

bath.

 It was then observed.

B. Preparation of Suspensoid

 In a beaker, with a 200ml of boiling water, 1ml of a saturated ferric chloride

solution was added. The result was then observed.


 We repeated the last two procedures in Part A and compared the obtained

results.

C. Foam Formation

 A 10 ml 0f 5% gelatin solution was placed in a test tube and shaken.

 Noted the result after 15 minutes if it formed a permanent foam.

 A 10 ml of colloidal ferric chloride solution (suspensoid) vigorously with air was

placed in a test tube then shaken and noted the result after 15 minutes.

D. Precipitation with Electrolytes

 Placed 5 ml of 5% gelatin solution in a test tube.

 Saturated Ammonium Sulfate was added dropped by dropped and noted the

number of drops, until permanent precipitate was formed.

 The procedure was repeated using colloidal ferric chloride instead of 5% gelatin

solution.

E. Reversibility

 In a 5ml of colloidal ferric chloride, 1ml of 10% MgSO4 solution was added in a

test tube and we let it stand for 30 minutes.

 The first procedure was repeated but using 5% gelatin solution instead of colloidal

ferric chloride.

 Solid MgSO4 was then added when there is no precipitate formed in gelatin

solution until it became saturated.


 The supernatant fluids was decanted from each of the two colloids.

 Excess water was then added and was noted if the two colloids were reversible or

not.

F. Precipitation of suspensoid particle with monovalent and bivalent ions which

carry charges of the opposite signs

 A 5ml of 1% colloidal ferric chloride was placed in a test tube.

 0.45N NaCl solution was then added dropped at a time (noted the number of

drops) then shaken it every addition of drops until a permanent precipitation is

formed.

 Procedure 1 is repeated but, using 1N solution of Na2SO4 and 1N MgCl2 instead

of 1% colloidal ferric chloride.

 The result was then compared.

G. Protective Action of Emulsoids

 A 5ml of 0.05N AgNO3 was placed in a test tube and was added with 5 drops of

concentrated nitric acid.

 5ml of 0.45N NaCl was then added in a solution and noted the copious

precipitate.

 Repeated the experiment but added 1ml of 5% gelatin solution for each 0.05N

AgNO3 and 0.45N NaCl before mixing.


III. RESULTS and INTERPRETATION

Procedure Observations

Upon mixing a gold colored solution was produced.


A. Preparation of an emulsoid
When cooled:
 It has gelatinized (formed in to solid state)
When heated:
 It returned into liquid state

Interpetation:

In this procedure 5% gelatin solution is used as an emulsoid. Emulsoid is a colloidal

dispersion in which the dispersed particles are more or less liquid and exert a certain attraction

on and absorb a certain quantity of the fluid in which they are suspended. It also has a lyophilic

characteristics in which enable to take up large amount of water which is seen as a gelatin form.

Because of the stability of an emulsoid and has a strong forces of attraction it produced a

gelatinized emulsoids when added tow water.

However when heated, the mixture of gelatin and water liquefies again by breaking the

bonds of a water and a colloid. Also, due to the elevation of temperature as it was reheated

causes the emulsoid to liquefy as its original form.

Procedure Observations

Formed reddish semi-solid chunks and settled in the


B. Preparation of Suspensoid bottom of the beaker.

When cooled: Did not dissolve in water

When heated: It dissolved in water and turned into a


clear solution
Interpretation:

Hydrolysis is a chemical process whereby a compound is cleaved into two or more

simpler compounds with the uptake of the H and OH parts of water molecule, this said we have

decided to use this technique in preparing the suspensoid in which the ferric chloride was reduce

to ferric hydroxide [ FeCl3 + 3H2O  Fe(OH)3 + 3HCl].

A lyophobic solution which is suspensoids has a lack of preference for the solvent that’s

why saturated ferric chloride failed to dissolve in the water because of having a slight affinity for

the dispersion medium. Iron is an example of an inorganic substances which are mostly

lyophobic which has a less stable and has a weak forces of attraction causing the colloids to not

easily mixed with the solvents.

Procedure Observation

C. Foam Formation Gelatin- Emulsoid: Formed a permanent foam after 15

minutes.

FeCl3- Suspensoid: Do not formed a permanent foam.

Interpretation:

A suspensoid as a lyophobic properties and has only a viscosity range as the same to the

dispersion phase that’s why it is not capable of forming permanent foam while, in terms of

emulsoid, the emulsoid has lyophilic properties and has a higher or greater viscosity than the

dispersion medium hence forming permanent foams. Emulsoids cannot easily return to its liquid

state thus the formation of foam retained.


Procedure Observation

D. Precipitation with Electrolytes Number of drops of ammonium sulfate added to

form a permanent precipitate

Gelatin- Emulsoid: Precipitate at 5 drops

FeCl3- Suspensoid: Precipitate at 37 drops.


Appearance of Precipitate

Gelatin- Emulsoid: Dark-brown precipitate

FeCl3- Suspensoid: No precipitate

Interpretation:

Emulsoid necessitate a large amount of electrolytes to form a precipitate only at high

concentration but, on the other hand, suspensoid does not form a precipitate for the reason that

they carry same charges with the solvent. It was precipitated by ions carrying charges of opposite

signs.
Procedure Observation

E. Reversibility Gelatin (emulsoid) FeCl3 (Suspensoid)

Precipitate after adding 1ml of10% No precipitate No precipitate

MgSO4

After 15 minutes then addition of solid Slightly liquefied No changes

MgSO4

After decantation and addition of excess Crystal Residue Completely Dissolved

water

Interpretation:

After decanting the dispersion plate which contains water, the residue which is a crystal

was reconverted into colloidal state when water was added again. This explains the reversibility

of an emulsoid. The stability of an emulsoids enables it to revers. In suspensoid, the forces are

too weak making them less stable and the residue is not convertible even after adding water, it

still remains irreversible.


Procedure 1 % Colloidal Ferric Chloride

F. Precipitation of # of drops Observation

suspensoid particle 0.45 N NaCl 1-15 1st drop caused turbidity- 15th drop caused
even turbidity
with monovalent 1 N Na2SO4 1-15 1st drop caused turbidity- 15th drop caused
even turbidity
and bivalent ions
1 N MgCl2 1-15 1st drop caused turbidity- 15th drop caused
even turbidity
which carry charges

of the opposite signs

Interpretation:

Procedure Observation

G. Protective 5 drops of concentrated 5 ml of 0.45 N NaCl

Action of nitric acid

Emulsoids 5 ml of 0.05 N 1 drop = turbidity Copious precipitate

AgNo3

1ml of 5% gelatin No precipitate No reaction

to NaCl and

AgNO3
Interpretation:

The particles of protective colloids is believed to be absorbed by lyophobic colloidal

particles and thus forms a covering over the surface of lyophobic colloidal particles. The

lyophobic colloid thus behaves as lyophilic colloid and is precipitated less easily by electrolytes.

IV. DOCUMENTATION

A.

PREPARATION OF EMULSOID
B.

PREPARATION OF SUSPENSOID

C.

FOAM FORMATION
D.

PRECIPITATION WITH ELECTROLYTES

E.

REVERSIBILITY
F.

PRECIPITATION OF SUSPENSOID PARTICLE WITH

MONOVALENT AND BIVALENT IONS WHICH CARRY CHARGES

OF THE OPPOSITE SIGNS

G.

PROTECTIVE ACTION OF EMULSOIDS


V. CONCLUSION

In conclusion, the experiment just shows that even if they are the same colloids, emulsoid

and suspensoid still varies in each perspective characteristics. For in fact, they seem almost the

polar opposite of each other.

Emulsoid is a lyophilic where it has affinity for the solvent, the water. During the

experiment, the prepared emulsoids was gelatinized due to the greater stability between two

molecules that enables them to interact or having the attraction with the solvent. But when the

temperature increases, it causes the gelatinized emulsoids to liquefy because the bonds between

the molecules was break due to higher temperature. While, a suspensoid is lyophobic, which

means that it has no affinity for the solvent making it less stable and cannot interact directly

between the colloidal particles and solvent particles which is water. This main characteristic is

what droves them apart.

In terms of having greater affinity to water, in forming permanent foam and forming

a precipitate emulsoid seems to have an advantage due to its inert character. It has a higher

viscosity than its dispersion medium compared to suspensoids. Emulsoids also can precipitate at

only with a high concentration. Thus, suspensoids does not form a permanent foam due to fact

that it has a same number of viscosity with its solvent. However, suspensoid showed that it can

precipitate with lower concentration of electrolytes.

Lastly, Emulsoids are said to be reversible mainly due to its stability while

suspensoids are not stable making the particles irreversible. This experiment had shown

exemplary opposite colloids and how they are differentiated.