RESEARCH PROPOSAL

A. Title page

Personal Data
Name Afdalia
Academic title S.Kep
Place and date of birth Maros, 10 January 1992
Nationality Indonesia
Position
Institution
Address Jl. Samudera Soreang, Maros
Telephone /fax 082339179971
Email afdalia_afdal@yahoo.com

Research
Proposal title Dengue Fever in children associated with Proteinuria
Research area Parasitology
Duration of Research 4 years

Supervisor
Name Dr. Sitti Wahyui,Ph.D.
Title Ph.D.
Position Senior lecture/ researcher
Institution Department of Parasitology
Faculty of Medicine
Hasanuddin University
Indonesia
Email sittiwahyuni@gmail.com
Dengue Fever in children associated with Proteinuria

Background
Dengue fever is an infectious disease carried by mosquitoes and caused by any of four
related dengue viruses. This disease used to be called break-bone fever because it sometimes
causes severe joint and muscle pain. Health experts have known about dengue fever for more
than 200 years. Dengue, also known as dengue fever, is a viral disease transmitted to humans by
mosquitoes of the genus Aedes. The incidence of dengue is currently increasing dramatically, and it is
now one of the diseases said to be re-emerging. WHO estimates the annual number of cases to be 50
million, including 500,000 cases of dengue hemorrhagic fever, which has a case fatality rate of over
2.5%. Two and a half billion people live in risk areas. Mostly, it seen only in the world's tropical and sub-
tropical regions. In Indonesia as a tropical regions, dengue fever incidents are increased rapidly in the last
20 years, almost all provinces and districts. The ministry of health in Indonesia estimated number case of
dengue from January to February 2016 are 8.487 cases with has a case of mortality 108. Mostly, the
suffer are children above 5-14 reach 43,44 % and 15-44 is 33,25% (Depkes, 2016).

The main form of severe dengue is characterized by plasma leakage with or without
bleeding, which may lead to circulatory collapse, called dengue shock syndrome. The course of
dengue illness can be divided into three main phases: the febrile phase, the critical phase and the
recovery phase. Severe clinical disease manifestations occur during the critical phase which
begins around day 4–7 after the onset of fever and lasts usually 48–72 hours. During the critical
phase, the condition of patients can improve or worsen rapidly; requiring careful monitoring by
care givers. Early clinical management based on fluid replacement therapy reduces the morbidity
and mortality associated with severe dengue.2 The major obstacle for an effective clinical
management of dengue is the inability to accurately predict, at an early stage of infection, which
patients are likely to develop a severe form of the disease. There is a need for simple, effective
and cheap tests to identify patients at risk and guide triage. (Wills et al). Observed an increase of
urinary protein clearance due to the increase in systemic vascular permeability that occurs in
severe dengue. Subsequently, it has been proposed that a simple urine protein excretion
screening test could be indicative of the severe form of dengue and therefore guide the triage and
monitoring of the patients with suspected dengue infection.

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 This study was to investigate the presence of proteinuria during dengue disease in
children by simple urinalysis strip and by protein:creatinine ratio (UPCR) and hopefully these
parameters can help the physicians to improve the clinical management of dengue.

RESEARCH PROBLEM STATEMENT

Increased urinary excretion of protein have a relationship with dengue fever. The
presence of proteinuria by using a dipstick, one of the easiest and cheapest methods for the
detection of proteins in urine but on observed that urinary dipstick used on samples collected at
the time of initial medical consultation before admission to the hospital was not a reliable tool for
the triage of dengue patients.
By this study, may we can answer what the gold standard to determine the presences of
proteinuria in dengue fever in children ?

OBJECTIVES
This study is going to determining the gold standar to measure of proteinuria in dengue
fever case and these parameters can help the physicians to improve the clinical management of
dengue

METHODOLOGY
This study use any methods to do. Clinical sample are patients with laboratory-confirmed
dengue virus infection (DVI) were randomly selected.Ethical statements use. The clinical
samples legal representatives signed a written consent before the enrolment of the patient. The
protein knew by urine analysis and statistical analysis.

OUTCOMES
This study will determining the gold standar to measure of proteinuria in dengue fever
case and these parameters can help the physicians to improve the clinical management of dengue

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REFERENCES

Depkes. 2016. Published on Monday, 07 Maret 2016

World Health Organization. Dengue haemorrhagic fever: diagnosis, treatment, prevention, and
control. 2nd ed Geneva: World Health Organization; 1997.