NAME

_______________________________________

Circle One: A1 A2

SCORE _____________ /16



MULTIPLE CHOICE
Circle the best answer for questions 1-10 (1 pt each)

1. A piece of double stranded DNA has 30% A, what will be the % of G?
a. 30%
b. 40%
c. 20%
d. 70%
e. not enough information to answer

2. You have a piece of DNA with the sequence ATGC. From this you know,
a. A is at the 5’ end
b. A is at the 3’ end
c. A could be at either the 5’ end or the 3’ end of DNA
d. You have a palindromic sequence
e. None of the above

3. Which of the following bases pairs with guanine?
a. Adenine
b. Guanine
c. Cytosine
d. Uracil
e. Thymine

4. The nitrogenous base is linked to the ribose or deoxyribose sugar via a
a. Glycosidic bond
b. Amide bond
c. Hydrogen bond
d. Phosphoanhydride bond
e. Phosphodiester bond

5. Genomic DNA is _______ resulting in the production of _______.
a. Transcribed mRNA
b. Translated, tRNA
c. Transcribed, DNA
d. Translated, protein
e. Replicated, mRNA




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6. _______ is the adaptor that is key to converting the triplet codons of mRNA into the
protein polymers.
a. tRNA
b. rRNA
c. mRNA
d. siRNA
e. lcRNA

7. Which of the following statements about double-stranded DNA is false?
a. The backbone contains alternating ribose sugar and phosphate groups.
b. The two strands have an antiparallel orientation.
c. The helix has two grooves called the major groove and the minor groove.
d. The bases are on the outside of the helix.
e. The strands are formed by nucleotides linked together via phosphodiester
bonds.

8. In the picture below, arrow labeled 1 corresponds to ______ and arrow 2 corresponds
to _________.
a. Replication, transcription
b. Translation, transcription
c. Mutation, replication
d. Transcription, replication
e. Transcription, translation








9. In the picture below, which label(s) on the left refer to the template strand?
a. 1
b. 2
c. mRNA
d. both 1 and 2 are template strands
e. both 1 and mRNA are the template strands

10. Which of the following statements are TRUE?
a. The mRNA is translated from 5’ to the 3’ end
b. The mRNA is translated from 3’ to 5’ end
c. During transcription, the mRNA is synthesized in the 3’à5’ direction
d. A and C are both true
e. B and C are both true

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SHORT ANSWER. Follow directions with each question.
11. For the base pair shown below, answer the following questions. (6 pts)
NH2 O

N HN
N

N O N
N
O
O
O
O
H H
H H
H H
O H H H
H O

a. Shown above is (circle all that apply).

Adenosine Guanosine Cytidine Uridine Thymidine

b. Draw hydrogen bonds between the bases as dashed or dotted lines.
c. CIRCLE the group that is the major difference between uridine and thymine.
d. Draw an arrow to the glycosidic linkage of the purine.
e. Draw a square around the 5’-carbon of the pyrimidine.

12. The following piece of double stranded DNA encodes a very short peptide. In the space
below, write the sequence of mRNA that would be synthesized by RNA polymerase from
this piece of DNA if the bottom strand is the template. Then write the protein sequence
in one-letter code that would be synthesized by the ribosome from that mRNA using the
first reading frame (use Genetic Code Table below). (0 pts)

5’- ATGGCTGGGTGCTTTTAA-3’
3’ –TACCGACCCACGAAAATT-5’

mRNA sequence:

AUG GCU GGG UGC UUU UAA

Protein sequence:

MAGCF