ABSTRAK

Dry Syrup adalah sediaan farmasi yang merupakan suatu campuran padat yang didispersikan
dengan air saat akan digunakan. Dry syrup amoksisilin adalah jenis antibiotik anak yang paling
banyak digunakan di Klinik Mirah Medika. Resistensi terhadap antibiotik telah menjadi isu
penting dalam bidang kesehatan khususnya farmasi. Penelitian ini bertujuan untuk
mengidentifikasi bakteri Escherichia coli pada dry syrup amoksisilin yang telah dilakukan
penambahan air selama 7 hari. Penelitian ini menggunakan 4 sampel dry syru pamoksisilin yang
berasal dari industri farmasi yang berbeda. Identifikasi dilakukan dengan pewarnaan gram dan
uji biokimia (uji IMViC dan fermentasi gula). Pewarnaan gram dilakukan untuk menentukan
spesifikasi bakteri gram negatif. Uji biokimia (uji IMViC dan fermentasi gula) dilakukan untuk
memastikan bakteri yang terdapat dalam dry syrup amoksisilin. Uji IMViC yang dilakukan
indole, methyl red, voges proskauer, sitrat, TSIA dan fermentasi gula (glukosa, laktosa dan
sakarosa). Data hasil penelitian ini dianalisis secara deskriptif. Hasil penelitian menunjukkan
bahwa pada hari ke-8 sampel tidak tercemar bakteri Escherichia coli, namun tercemar bakteri
gram negatif lain. padahari ke-16, 2 sampel positif tercemar Escherichia coli, sedangkan 2
sampel lain tercemar bakteri gram negatif lainnya. Dari hasil penelitian yang dilakukan dapat
disimpulkan bahwa dry syrup yang terdapat di Klinik Mirah Medika telah tercemar bakteri lain
setelah hari ke-7 dan terdapat 2 sampel yang tercemar bakteri Escherichia coli pada hari ke-16.

Kata kunci :Escherichia coli, dry syrup amoksisilin, identifikasi bakteri.

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 ABSTRACT

Dry Syrup is a pharmaceutical preparation which is a solid mixture which is dispersed with water
when it is to be used. Dry syrup amoxicillin is the most widely used type of pediatric antibiotic
in the Mirah Medika Clinic. Resistance to antibiotics has become an important issue in the health
sector, especially pharmaceuticals. This study aimed to identify Escherichia coli bacteria in
amoxicillin dry syrup which had been added to water for 7 days. This study used 4 samples of
dry pamoxicillin syringes from different pharmaceutical industries. Identification was carried out
by gram staining and biochemical tests (IMViC test and sugar fermentation). Gram staining is
done to determine the specifications of gram negative bacteria. Biochemical tests (IMViC test
and sugar fermentation) were carried out to confirm the bacteria found in dry syrup amoxicillin.
The indole, Methyl red, proskauer, citrate, TSIA and fermented sugar (glucose, lactose and
saccharose) vesicles. Data from the results of this study were analyzed descriptively. The results
showed that on the 8th day the samples were not contaminated with Escherichia coli bacteria, but
were contaminated with other gram negative bacteria. on the 16th day, 2 positive samples were
contaminated with Escherichia coli, while 2 other samples were contaminated with other gram
negative bacteria. From the results of the research conducted it can be concluded that the dry
syrup found at the Mirah Medika Clinic was contaminated with other bacteria after the 7th day
and there were 2 samples contaminated with Escherichia coli bacteria on the 16th day.

Keywords: Escherichia coli, dry syrup amoxicillin, identification of bacteria.

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