TITLE

EXPERIMENT 7 : Determination of volatile fatty acid number (VFA NO.)

PROGRAM & GROUP: AS113 & RAS1133A

COURSE: PST 221

DATE EXPERIMENT: 22/10/2018

NAME MATRIX NO

MUHAMMAD IRFAN BIN AZWARI 2017256762

MUHAMMAD IQBAL BIN INDRA MUFELI 2017248854

RAJA MUHAMMAD ARASHRIFQI BIN RAJA ADNAN 2017256794

MUHAMMAD DARWIN BIN MOHMAD ROSDI 2017256596

MUHAMMAD NAJMI BIN AZHAR KHAIRI 2017256546

MOHAMMAD FARID BIN MOHAMMAD AZIS 2017256786

LECTURER: MADAM SITI NOOR ASHIKIN

Objective

To determine the volatile fatty acid number (VFA NO.) of natural rubber latex

Apparatus

- Stainless steel beaker

- Markham still

- 50 ml conical flask

- Filter paper

Chemical

Latex concentrate latex

30% w/v ammonia sulphate solution

50% w/v sulphuric solution

0.01N Barium Hydroxide

Phenolphalein

Procedure

1. Weight 50 gram of concentrate latex in stainless beaker

2. Add 50 ml 30% w/v ammonia sulphate solution and stir mixture with steel rod

3. Place beaker in water bath and stir slowly until latex coagulate and the serum is

clear
4. Press or squeeze the serum from the coagulum and filter the serum by using a

folded filter paper

5. Pipette 25 ml filtrate into 50 ml conical flask with stopper

6. Add 5 ml 50% w/v sulphuric acid into filtrate and swirl

7. Clean markham still

8. Introduce 10 ml acidified serum by pipette into inner tube of the markham still

with steam outlet

9. Add 1 to 2 drop anti foam agent , washing it down with distilled water

10. Replace stopper the markham still and place a graduated 250 ml conical flask

under the tip of the condenser to reveive the distillate

11. Pass steam gently and close the steam outlet to divert steam into the inner tube

12. Adjust the flow of steam to produce the distillate at a rate of 3 to 6 ml per minute

until 100 ml of the distillate has been collected

13. Aerate the distillate by passing through the distillate a stream of co2 free air at a

ra5e 200 to 300 ml per minute for approximately 3-6 minute

14. Add a drop of phenolphthalein and titrate carefully with 0.01N (0.005 mol) barium

hydroxide

15. The end point is faint permanet pink