ANNEX – C1

LIST OF TEST FACILITIES FOR CHEMICAL TESTS OF PACKAGED DRINKING WATER AS PER IS 14543:2004

Sl. Tests Clause Ref. of Referred Method of Test/ Test Equipment/Apparatus* Chemicals Remarks
No. IS 14543:2004 Refer to IS
1. Colour Clause 5.2 & Sl. No. i) IS 3025 (part 4):1983 ● Nessler cylinders (50 ml) ● Potassium -
of Table 1 i) Platinum cobalt ● Centrifuge or filter Chloroplatinate
(Visual comparison) method assembly (functional pore size ● Cobaltous
0.45µm) Chloride,
Crystalline
● Conc.
Hydrochloric acid
● Distt. Water
-
ii) Spectrophotometric ● Spetrophotometer (400 to ● Conc. Sulphuric
Method 700 (nm) with 10 mm absorption acid
cell ● Sodium hydroxide
● Filteration system
consisting of filteration flask
with side tubes;
crucible holder;
Micrometallic filter
Crucible (pore size 40 (µm);
Calcined filter aid (celite 505 or
equivalent) and
Vacuum system
● Refrigerator (recommended)
● pH meter
● Centrifuge
*Note: Besides listed Equipments/Apparatus/Chemicals, following accessories are essential part of a chemical lab:
 i) General glass wares like Pipettes Burette, Conical flasks, Beakers, Measuring cylinders, Volumetric flasks, (of different volumes)
ii) Provision for distilled/double distilled water
iii) Fuming Hood and sink with tap in the lab
# The list does not cover the requirements of Pesticide Residues and Radio Active Residues as these requirements are got to be tested
from outside approved lab.

The requirements having frequency of testing as once a month or more may be got tested from outside lab like Fluoride, Silver
Selenium, Sodium, Phenolic Compounds, Mineral Oil, Antimony, Borate, Mercury, Cadmium, Arsenic, Cyanide, Lead, Chromium,
Nickel, Poly-nuclear aromatic hydrocarbon (PAH), poly chlorinated bi-phenyl (PCB)

2. Odour Clause 5.2 and Sr. No. IS 3025 (Pt 5):1983 ● Wide mouth glass stoppered ● Odour free -
ii) of Table 1 bottles (approx. 1 lit. distilled water (or
capacity) distilled water and
column of
granulated
activated carbon)
● Hydrochloric acid
3. Taste Clause 5.2 and Sr. No. IS 3025 (Pt 8):1984 • Breaker (50 ml) • Taste and Odour -
iii) of Table 1 • Water bath free water
• Thermometer • 2000 mg/l solution
of sodium chloride
4. Turbidity Clause 5.2 and Sl. No. IS 3025 (Pt 10):1984 • Sample tubes • Distilled water -
iv) of Table 1 • Turbidimeter • Hexamethylene
• Volumetric flasks (100 ml) Tetramine
• Membrane filter with pore • Hydrazine sulphate
size not more than 0.45 µm
5. Total Clause 5.2 and Sr. No. IS 3025 (Pt 16):1984 • Filter: -
dissolved v) of Table 1 Glass fibre filter Disc
solids (whatman GF/C or
 equivalent) 2.1 to 5.5 cm in
diameter, pore size 1.2 µm
OR
Ashless Filter Paper- pore size
2 to 2.5 µm equivalent to
whatman filter No. 542
OR
Gooch crucible (whatman or
equivalent)
OR
Sintered Disc (G-5 or
equivalent) Pore size 1 to 2
µm
OR
Membrane filter (0.45 µm
membrane)

ƒ Filtering Assembly
(suitable for type of filter
selected)

• Drying oven (180 ± 2˚C)

• Desiccator
• Analytical balance (200 g
capacity and l.c. 0.1 mg)
• Pipettes
• Evaporating dish
• Magnetic stirrer
(recommended)
6. pH Clause 5.2 and Sr. No. IS 3025 (Part 11):1983 • pH meter – with glass and
vi) of Table 1 reference electrode
1. Electrometric method (saturated calomel) l.c 0.1
• Magnetic stirrer with
polytetrafluoro ethylene
coated stirring bar
• Thermometer (l.c. 0.5°C)
• Beakers
• Standard pH Buffer -
solutions/tablets
(Minimum two
different values)
OR
Distilled water
Borax (for Borax
buffer)
Potassium dihydrogen
phosphate, Sodium
hydrogen phosphate
and oven (for
phosphate buffer)
Potassium hydrogen
tartrate (for Tartrate
buffer)
Potassium hydrogen
phthalate (for Phthalate
buffer)
Potassium tetraoxalate
dihydrate (for
Tetraoxalate buffer)
Calcium Carbonate
Platinum dish, Muffle
furnace, Hot Plate,
Fritted glass filter of
medium porosity,
polyethylene bottle,
Suction pump & fritted
glass funnel (for Tetra
oxalate buffer)
2. Colorimetric Method • Hard glass tubes • Methyl orange, -
methyl red,
bromothymol blue,
phenolphthalein
and alcohol (66%)
(for universal
indicator)
• Thymol blue
indicator (acid
range)
• Bromophenol blue
indicator
• Bromocresol green
indicator
• Methyl red
indicator
• Bromocresol purple
indicator
• Bromothymol blue
indicator
• Phenol Red
indicator
• Cresol Red
indicator
• Thymol Blue
(alkali range)
indicator
• Thymolphthalein
indicator

7. Barium Clause 5.2, Sl. No i) i) Annex F of IS 13428:1998 • Filter paper and filtration
of Table 2 assembly
• Hot plate/gas burner
ii) IS 15302:2003 • Atomic Absorption
Spectrophotometer and
Associated equipment
(Burner, Readout
mechanism, lamp for
Barium, Pressure Reducing
valves and vents)
• Nitrous oxide burner head
• T-junction valve or other
switching valve
• Air (compressor or
commercially bottled gas)
• Thymol violet
indicator
• Different buffer
solutions of known
pH
• Ammonium -
Dichromate
• Ammonium
Acetate
• Ammonium
Hydroxide
• Potassium Iodide
• Sodium
Thiosulphate(0.1N)
• Hydrochloric Acid
• Ammonium
Chloride
• Starch indicator
• Metal free water -
• Hydrochloric Acid
• Nitric Acid
• Sulphuric Acid
• Hydroflouric Acid
• Potassium Chloride
• Aluminium Nitrate
• Standard barium
solution 100µg/ml
(Barium chloride,
oven, hydrochloric
acid)
 • Acetylene Gas, Standard
Commercial grade
• Nitrous oxide gas
8. Copper Clause 5.2, Sr. No. ii) IS 3025 (Part 42):1992 ● Spectrophotometer & 1cm • Ammonium Detectio
of Table 2 i) Neocuproine Method cell Hydroxide n range
● Hot plate • Chloroform, AR 0.05 to
● Separating funnels (125 ml) Grade 5.0mg/l
• Conical flasks • Hydrochloric acid,
Conc.
• Hydroxylamine
Hydrochloride
• Isopropyl Alcohol
• Neocuproine
• Double Distilled
water
• Nitric Acid, Conc.
• Sulphuric Acid,
Conc.
• Hydrated Sodium
Citrate
• Stock copper (II)
solution 200µg/ml
(Pure Copper
Metal, hot plate)
• Hydrogen Peroxide
ii) Atomic Absorption • Atomic Absroption • Hydrochloric Acid, Detectio
Method (Direct) Spectrophotometer with Conc. n range
air-acetylene flame • Nitric Acid, Conc. 0.02 to
• Copper Hollow Cathode • Dilute Sulphuric 5.0mg/l
lamp Acid
 • Stock copper (II)
solution –
1.0mg/ml (Pure
Copper metal &
hot plate)
iii) Atomic • Atomic Absorption • Hydrochloric Acid, Detectio
Absorption Spectrophotometer with Conc. n range
Method air-acetylene flame • Nitric Acid, Conc. 0.002 to
(Chelation • Copper Hollow Cathode • Pyrrolidine 0.5 mg/l
(Extraction) Lamp • Methyl Isobutyl
• Separating Funnel Ketone - Reagent
• Volumetric Flasks grade (MIBK)
• Distillation Assembly • Carbon Disulphide
• Sodium Hydroxide
• Distilled water
• Water Standard
MIBK
• Bromophenol Blue
• Ethanol or
Isopropanol
• Stock copper (II)
solution – 1.0mg/ml
(Pure Copper metal
& hot plate)
Sulphuric Acid

• Hydrochloric Acid
 Conc.
iv) Differential Pulse (Spectro Grade)
Anodic Stripping • Polarograph capable of • Nitric Acid-Conc. Detectio
Voltametry performing differential (Spectro Grade) n range
pulse work • Sulphuric Acid 0.0001
• Hanging Mercury Drop Conc. to
Electrode • Stock copper (II) 0.1mg/l
• Platinum Counter solution – 1.0mg/ml
Electrode (Pure Copper metal
• Saturated Calomel & hot plate)
Reference Electrode • Amalgamated Zinc
• Magnetic Stirrer Control (Granular Zinc and
unit with Stirring Bar Mercury)
• Scrubber • Ammonium Meta
• Whatman Filter Paper No. Vanadate
40 • Distilled water
• Purified Nitrogen Gas • Hydrochloric Acid-
Conc. (Containing
less than 0.00005%
IS 3025 (Pt. 53) :2003
iron)
9. Iron Clause 5.2 and (1) 1,10 Phenanthroline i)Detecti
• Spectrophotometer • Hydroxylamine
Sr. No iii) of Table 2 Method on range
• Std. volumetric Hydrochloride
0.075 to
Glass wares • Ammonium 0.5mg/l
• Hot Plate Acetate
• Beakers • Glacial Acetic Acid ii) This
• Volumetric Flasks • Sodium Acetate require
• Pipettes • 1,10 Phenanthroline ment is
• Conical flasks Monohydrate not
• Separating Funnel • Stock Iron Solution applicab
• Fuming Hood 1ml=200µg of Fe le for
 • 0.45µ m Membrane Filter (Conc. Sulphuric Package
with Filtration Assembly Acid, Ferrous d
Ammonium Natural
Sulphate, Mineral
Potasssium Water
Permanganate)
• Std. Iron Solution
(1.0 ml=1.0µg of
Iron)
• Di-isopropyl Ether
(2) Atomic Absorption • Atomic Absorption • Distilled water Detectio
Method (DIRECT) Spectrophotometer • Hydrochloric Acid n range
• Air Acetylene Flame – Conc. 0.1 to 10
• Iron Hollow Cathode Lamp • Nitric Acid – Conc. mg/l
or Electrodeless discharge • Sulphuric Acid –
lamp for use at 248.3nm Conc.
• Volumetric Flasks • Calcium Chloride
Solution (Calcium
Carbonate,
Hydrochloric acid)
• Stock Iron Solution
(1.0 ml=100µg of
fe) (Pure iron wire,
Hydrochloric acid
Nitric Acid)
IS 15303:2003 • Atomic Absorption • Metal free water Minimu
3. Electrothermal Atomic Spectrometer • Hydrochloric Acid m
Absorption Spectrometric • Hollow Cathode lamp for Conc. detectio
Method Iron • Nitric Acid, Conc. n limit
• Graphite Furnace • Matrix Modifier 0.001mg
 • Readout Mechanism stock solutions /l
• Sample Dispenser (Magnesium
• Vent for fumes Nitrate, Nickel
• Cooling device Nitrate, Phosphoric
• Membrane Filter Acid, Palladium
Apparatus (0.45µm) Nitrate & Citric
Acid)
• Stock iron Solution
– 100µg/ml (Iron
wire)
• Sodium hydroxide
10N
• Chelating resin

10. Manganes Clause 5.2, Clause 35 of IS 3025:964 • Nessler’s Tubes • Sulphuric Acid Detectio
e Sl. No. iv), Table 2 • Beakers • Hydrogen Peroxide n limit
• Hot Plate (30%) up to
• Volumetric flask • Nitric Acid, Conc. 0.2mg/l
• Pipettes • Stabilized Distilled
• Conical Flasks Water
• Burette OR
Distillation Assembly,
Distilled water,
Potassium
Permanganate and Dil.
Sulphuric Acid
• Phosphoric Acid
(sp. Gr. 1.75)
• Potassium
Periodate
 • Std. Manganese
Solution (1ml=0.02
mg of Mn)
(Standard 0.1 N
Potassium
Permanganate
solution, saturated
solution of sulphur
dioxide)

11. Nitrate (as Clause 5.2, Sl. No. (v) IS 3025 (Part 34):1988 • Reduction Column • Distilled water Detectio
NO3) of Table 2 i) Cadmium • Colorimeter • Nitrate free water n limit
Reduction - Spectrophotometer • Cadmium granules maximu
Method OR (40 – 60 mesh) m 0.1
Filter photometer • Hydrochloric Acid mg/l
• Glass wool (6N)
• 0.45 µ m pore diameter • Copper Sulphate
membrane filter Solution
• Refrigerator • Sulphanilamide
• Conc. Hydrochloric
Acid
• N-(1-napthyl))-
Ethylenediamine
dihydrochloride
(NED)
Dihydrochloride)
• Ammonium
Chloride
• Disodium Ethylene
diamine tetra
 acetate
• Ammonia Solution
• Copper sulphate
Solution – 2%
• Stock nitrate
solution – 100µg/ml
(Potassium Nitrate
& Chloroform)
• Chloroform
• Stock nitrite
solution - 100µg/ml
(Potassium Nitrite
& Chloroform)
• Nitrite free water

ii) Chromotropic Acid • Spectrophotometer • Nitrate free water Detectio

Method OR • Stock Nitrate n range
Photometer Solution - 100µg/ml 0.1 to
• Pipettes (Potassium 5.0mg/l
• Volumetric flasks Nitrate,
Chloroform)
• Standard Nitrate
solution –
10.0µg/ml
• Sulphite Urea
Reagent
(Urea & Anhydrous
sodium Sulphite)
• Antimony reagent
(Antimony metal,
 Conc. Sulphuric
acid)
• Chromotropic Acid
Reagent (Purified
chromotropic Acid
crystals, Conc.
Sulphuric Acid)
• Sulphuric Acid,
Conc. Nitrate free

3) Devarda’s Alloy • Distillation Assembly • Ammonia Free Detectio

Reduction Method (Kjeldahl Assembly) Water n limit
• Measuring Scoop • Borate Buffer minimu
• Spectrophotometer Solution (0.1N m 2mg/l
OR Sodium Hydroxide,
Photometer 0.025M Sodium
• Volumetric Flasks Tetraborate)
• Sodium Hydroxide
–6N
• Devarda’s Alloy –
20 mesh with less
than 0.005 percent
Nitrogen
• Mixed indicator
Solution
(Methyl Red
indicator, Ethyl
Alcohol/Isopropyl
Alcohol, Methylene
Blue)
 • Indicating Boric
Acid Solution
(Hydroboric Acid,
mixed indicator
solution)
• Std. Sulphuric Acid
Titrant - 0.02 N
• Nessler’s Reagent
(Mercuric Iodide,
Potassium Iodine.
Sodium Hydroxide)
• Stock Ammonia
Solution -1.22mg
ammonia/ ml
(Anhydrous
Ammonium
Chloride)
• Standard Ammonia
Solution

12. Nitrite Clause 5.2 and Sr. No. IS 3025(Part 34) :1988 • Spectrophotometer / • Nitrite Free water -
vi) of Table 2 Photometer (Distilled water,
OR Potassium
Nessler’s cylinders Permanganate,
method Barium
• Nessler’s Tubes Hydroxide/Calcium
• 0.45 µm Membrane Filter Hydroxide Conc.
• Distillation Assembly Sulphuric Acid,
(borosilicate) Manganese
Sulphate)
• Sulphanilamide
Reagent
• NED
Dihydrochloride
• Hydrochloric Acid
• Sodium Oxalate –
0.05 N.
• Ferrous
Ammonium
Sulphate – 0.05N
(Ferrous
Ammonium
Sulphate, Conc.
Sulphuric Acid,
Std. Dichromate
solution)
• Stock Nitrite
Solution - 250µg of
nitrogen/ml
(Sodium Nitrite,
Chloroform,
Sodium Oxalate,
Std., Potassium
Permanganate
solution)
• Intermediate Nitrite
Solution –
50.0µg/ml
• Standard Nitrite
Solution –
 0.500µg/ml

13. Flouride Clause 5.2; Sr.No. vii) Clause 23 of IS 3025:1964 • Nessler Tubes (100ml) • Sodium Thiosulphate -
of Table 2 • Distillation Apparatus Solution (0.1 N)
• Refrigerator • Standard Sodium
(Recommended) Fluoride Solution (1ml
• Heating mantle = 0.01 mg F)
• Zirconium Oxychloride
OR
Zirconium Oxynitrate
• Alizarin Sodium
Monosulphonate
(Alizarin S)
• Conc. Hydrochloric
Acid
• Conc. Sulphuric Acid
• Silver Sulphate
• Perchloric Acid
• Phenolphthalein
Indicator
• Sodium Hydroxide
Solution
14. Zinc Clause 5.2; Sr.No. viii) IS 3025 (Part 49): 94 • Spectrophotometer (620nm • Sodium Hydroxide Detectio
of Table 2 with 1cm cells) • Potassium Cyanide n range
i) Zincon Method • Chlorohexanone 0.02 to 5
• Distt. Water mg/l
• Zincon
• Methanol
• Sodium Ascorbate
• Borate Buffer Solution
 (Sodium Hydroxide,
Potassium Chloride,
Boric Acid)
• Hydrochloric Acid,
Conc.
• Zinc Sulphate

• Hydrochloric Acid,
ii) Atomic Absorption • Atomic Absorption Conc. Detectio
Method (Direct) Spectrophotometer with • Nitric Acid, conc. n range
Air-Acetylene Flame • Stock Zinc Solution – 0.01 to
• Hollow Cathode Lamp 1.0mg/ml (Zinc 2.0mg/l
Or Electrodeless discharge Granules/Zinc Oxide)
lamp

• Hydrochloric Acid,
iii)Atomic Absorption • Atomic Absorption Conc. Detectio
Method (Chelation – Spectrophotometer with n range
• Nitric Acid, Conc.
Extraction) Air-Acetylene Flame 0.001 to
• Pyrrolidine Dithio
• Hollow Cathode Lamp 0.2mg/l
Carbamic Acid -
Chloroform Reagent
(Pyrrolidine,
Chloroform, Carbon
disulphide)
• Sodium Hydroxide
• Chloroform
• Bromophenol Blue
Indicator (Bromophenol
 Blue, Ethanol or
Isopropanol)
• Stock Zinc (II)
Solution- 1.0 mg/ml
(Zinc Granules or Zinc
Oxide, Nitric Acid)

iv) Differential Pulse Anodic • Polarographic • Hydrichloric Acid,

Stripping Voltammetry Instrumentation Conc. Detectio
(DPASV) Method Capable of Performing • Nitric Acid, Conc n range
Differential Pulse • Stock Zinc Solution - 0.001 to
Work 1.0mg/ml 0.1mg/l
• Hanging Mercury Drop • Amalgamated Zinc
Electrode (Granular Zinc, Conc.
• Platinum Counter Hydrochloric Acid,
Electrode Mercury)
• Saturated Calomel • Purified Nitrogen
Reference Electrode (Ammonium Meta
• Magnetic Stirrer Vanadate, Scrubber,
Amalgamated Zinc,
Nitrogen Gas)
15. Silver Clause 5.2; Sr.No. ix) Annex J of IS 13428:1998 • Atomic Absorption • Deionised Distilled -
of Table 2 Spectrophotometer with Water (Ion Exchange
Oxidizing Air Acetylene Column & Distilled
Flame Water)
• Nitric Acid – Redistilled
• Hydrochloric Acid –
Redistilled
• Silver Std. Solution
 (Silver Nitrate)
• Lanthanum Chloride
• Lanthanum Stock
Solution (Lanthanum
Oxide, Hydrochloric
Acid)
• Ammonium Pyrrolidine
Dithiocarbamate
solution)
• Methyl isobutyl ketone
16. Aluminiu Clause 5.2; Sr.No. x) i) IS 3025(Part • Spectrophotometer (535 • Sulphuric Acid – 0.02 N i)Detecti
m of Table 2 55):2003 nm with 1cm Cells) and 6 N on range
• pH Meter • Ascorbic Acid Solution 0.02 to
a) Eriochrome Cyanine R • Standard Volumetric • Buffer Solution 0.3mg/l
Method Glasswares (Sodium Acetate & 1 N
Acetic Acid) ii) This
• Acetic Acid Solution – require
1:1 and 1 N ment is
• Sodium Hydroxide not
Solution – 0.1 N and 1N applicab
• Stock Eriochrome le for
Cyanine R Dye Solution Package
d
• Stock Aluminium
Natural
Solution – 500 µg/l
Mineral
(Aluminium Potassium
Water
Sulphate)
• Methyl Orange
Indicator Solution
b) Atomic Absorption • Atomic Absorption • Hydrochloric Acid, Detectio
Method (Direct) Spectrophotometer with Conc. n range
 Nitrous Oxide – Acetylene
Flame and Hollow-
Cathode Lamp
• Standard Volumetric
Glasswares
ii) IS 15302:2003 Direct • Atomic Absorption
Nitrous Oxide – Acetylene Spectrometer
Flame Atomic Absorption • Burner
Spectrometry • Read Out Mechanism
• Lamp (Hollow Cathode or
EDL)
• Pressure Reducing Valves
• Vent
• Nitrous Oxide Burner Head
• T-Junction Valve or Other
Switching Valve
• Air (Compressor or
Bottled Gas)
• Acetylene, Standard
Commercial Grade
• Nitrous Oxide Gas
17. Chloride Clause 5.2; Sl.No.xi) IS 3025 (Part 32):1988 • Erlenmeyer Flask (250ml)
of Table 2 • Burette
i) Argentometric Method
• Nitric Acid, Conc. 5 to
• Potassium Chloride 100mg/l
Solution
• Stock Aluminium
Solution - 500 µg/l
(Aluminium Potassium
Sulphate)
• Metal Free Meter Detectio
• Hydrochloric Acid – 1 N n limit
• Nitric Acid, Conc. 0.1mg/l
• Sulphuric Acid
• Hydrofluoric Acid – 1 N
• Potassium Chloride
• Aluminium Nitrate
• Standard Aluminium
Solution - 100 µg/l
(Aluminium Metal)
• Potassium Chromate -
Indicator Solution
(Potassium Chromate,
Silver Nitrate)
• Standard Silver Nitrate

ii) Mercuric Nitrate Method
• Erlenmeyer Flask (250 ml)
• Microburette (5 ml with
l.c. 0.01ml)
• Refrigerator
• pH meter
Solution – 0.01 N(silver
nitrate, sodium chloride)
• Standard Sodium
Chloride Solution –
0.01 N (Sodium
Chloride)
• Aluminium Hydroxide
Suspension (Aluminium
Potassium Sulphate or
Aluminium Ammonium
Sulphate, Conc
Ammonium Hydroxide)
• Phenolphthalein
Indicator Solution
• Sodium Hydroxide – 1N
• Sulphuric Acid – 1N
• Hydrogen Peroxide –
30%
• Standard Sodium
-
Chloride Solution –
0.01N (sodium
chloride)
• Nitric Acid – 0.1N
• Sodium Hydroxide –
0.1N
• Indicator – Acidifier
Reagent (S-
 Diphenylcarbazone,
Conc. Nitric Acid,
Xylene Cyanol FF,
Ethyl Alcohol or
Isopropyl Alcohol)
• Standard Mercuric
Nitrate Solution –
0.01N (Mercuric
Nitrate, Conc. Nitric
Acid, Sodium
Bicarbonate, Std.
Sodium Chloride
Solution)
• Mixed Indicator
Reagent
(Diphenylcarbazone,
Bromo Phenol Blue,
Ethyl Alcohol or
Isopropyl Alcohol)
• Standard Mercuric
Nitrate Solution – 0.1N

iii) Potentiometric Method • Glass and Silver- Silver • Standard Sodium -

Chloride Electrodes Chloride Solution
• Electronic Voltmeter (0.01N)
• Mechanical Stirrer • Nitric Acid-Conc
• Standard Silver Nitrate

• Automated Analytical
iv) Automated Ferricyanide Equipment
Method • Filters (480nm)
18 Selenium Clause 5.2; Sr.No. xii) i) IS 3025 (Part 56):2003 • Spectrophotometer
of Table 2 (480nm)
a)Spectrophotometric • Volumetric Glasswares
Method • Separating Funnel (250ml)
Preferably Flourocarbon
Stopcock
• Water Bath –
Thermostatically
Solution (0.01N)
• Pretreatment Reagent
(Sulphuric Acid,
Hydrogen Peroxide,
Sodium Hydroxide –
1N)
• Stock Mercuric -
Thiocyanate Solution
(Mercuric Thiocynate,
Methanol)
• Stock Ferric Nitrate
Solution (Ferric
Nitrate, Conc. Nitric
Acid)
• Colour Reagent (Poly
oxy Ethylene 23 Lauryl
Ether)
• Sodium Chloride
• Stock Selenium Detectio
Solution – 1.0mg/ml n limit
(Sodium Selenite, minimu
Hydrochloric Acid) m
• Hydrochloric Acid – 0.01mg/l
0.1N
• Ammonium Hydroxide
• Cyclohexane
 Controlled • 2,3 –
• pH Meter Diaminonaphthalene
• Centrifuge (DAN)
• Centrifuge Bottles with • Hydroxylamine
Flourocarbon Screw Cap Hydrochloride
• Sodium Salt of EDTA
• Amberlite XAD -8 or
Equivalent Resin
• Hydrochloric Acid,
Conc
• Potassium Hydroxide

b) Atomic Absorption • Atomic Absorption -

• Nitric Acid
Spectrometric Method Spectrometer Fitted with • Sulphuric Acid
(Hydride Technique) Hydride System and • Hydrochloric Acid
Hollow Cathode • Hydrogen Peroxide
Lamp/Electrodeless
• Sodium Hydroxide
Discharge Lamp
• Sodium Tetrahydro
• Gas (Argon or Nitrogen) borate
• Glasswares
• Selenium Stock
• Decomposition Apparatus Solution (1mg/ml)
(Round Bottom Flask, (Selenium Dioxide)
Reflux Condenser,
Condensate Reservoir)
 ii) IS 15303:2003
Electrothermal Atomic • Atomic Absorption • Metal Free Water Detectio
Absorption Spectrometric Spectrometer • Hydrochloric Acid, n limit
Method • Hollow Cathode Lamp or Conc minimu
Electrodeless Discharge • Nitric Acid, Conc m
Lamp • Matrix modifier Stock 0.002mg
• Graphite Furnace Solutions /l
• Readout Mechanism (Magnesium
• Sample Dispenser Nitrate, Nickel
• Vent for Fumes Nitrate, Phosphoric
• Cooling Device Acid, Palladium
• Membrane Filter Nitrate, Citric Acid)
Apparatus (0.45 µ m) • Stock Metal Solution –
1mg/ml (Sodium
Selenite)
• Chelating Resin – 100
to 200 mesh
19 Sulphate Clause 5.2; Sr.No. xiii) IS 3025 (Part 24):1986 • Steam Bath • Methyl Red Indicator Detectio
of Table 2 • Drying Oven • Hydrochloric Acid n limit
i)Gravimetric Method • Muffle Furnace • Barium Chloride more
• Desiccator • Silver Nitrate than
• Analytical Balance • Nitric Acid 10mg/l
(l.c.0.1mg) • Ion Exchange Resin
• Filter Paper (Preferably (Amberlite IR-120 or
Whatman No.42) Equivalent)
• Silica or Porcelain Crucible
• Ion Exchange Column
• Filter (0.45 µ m)
• Platinum Dish
 • White Porcelain Basin • Ethyl Alcohol
ii) Thorin Method • Burette • Ammonium Hydroxide Detectio
• Ion Exchange Column (Ammonia-Conc n range
• Filter – 0.45µm and Distilled 5 to
Water) 150mg/l
• Hydrochloric Acid
• Thorin (2,2 – Hydroxy
– 3,6 – Disulpho –
1 – Naphthylazo
Benzene Arsenic
Acid)
• Ion Exchange Resin
(Amberlite IR-120
or Equivalent)
• Stock Sulphate
Solution – 100 mg/l
(Anhydrous
Sodium Sulphate)

• Barium Chloride –
Standard Solution
(Barium chloride m
hydrochloric acid
ammonia)

• Turbidity Meter or • Barium Chloride

iii)Turbidity Method Spectrophotometer • Gelatin Powder Detectio
 • Glass Apparatus • Glycerol n limit 1
• Hot Plate • Hydrochloric Acid, to
• Refrigerator Conc 40mg/l
(recommended) • Sodium Chloride
• Filter – 0.45µm • Ethyl or Isopropyl
Alcohol
• Anhydrous Sodium
Sulphate
• Stock sulphate solution
– 100mg/l
20 Alkalinity Clause 5.2; Sr. No.xiv) IS 3025 (Part 23):1986 • pH Meter • Distilled Water Detectio
of • Burette • Sulphuric Acid, Conc n range
Table 2 i) Indicator Method • Magnetic Stirrer Assembly • Standard Solution of 0.5 to
Sulphuric Acid – 0.02 N 500mg/l
• Phenolphthalein
Indicator
• Mixed Indicator
Solution (Methyl Red,
Bromocresol Green,
Ethyl or Isoprophyl
Alcohol)

ii)Potentiometric Method • Potentiometer • Standard Sulphuric Detectio

• Glasswares Acid – 0.02N n range
0.5 to
500mg/l
21 Calcium Clause 5.2; Sr. No.xv) IS 3025 (Part 40):1991 • Hot Plate • Sodium Hydroxide -
of Table 2 • Glasswares Solution – 1N
i)EDTA Titrimetric Method • Polyethylene Bottle • Hydrochloric Acid –
 0.1N
• Indicator
Solution:Murexide
(Ammonium Purpurate)
Indicator, Absolute
Ethylene Glycol
Sodium Chloride
OR
Patton and Reeder’s
Indicator (Eriochrome Blue
Black R, Sodium
Sulphate/Potassium
Sulphate)
• Standard EDTA
Solution – 0.01M
(Disodium Ethylene
Diamine Tetra – Acetate,
Standard Zinc Solution,
(Or Standard Calcium
Solution) Buffer Solution,
Eriochrome Black T
Indicator Solution
• Stock Calcium Solution
(Calcium Carbonate,
Hydrochloric Acid –
0.1N)
• Nitric Acid, Conc

• Atomic Absorption
 ii)Atomic Absorption Spectrometer with • Hydrochloric Acid – Detectio
Spectrometric Method Air/Acetylene or Nitrous 1N and 0.1N n limit
Oxide/Acetylene Flame • Lanthanum Chloride maximu
and Hollow Cathode Lamp • Cesium Chloride m
(Calcium) • Standard Calcium 50mg/l
Solution

• Beakers, Cover Glass, and

iii) Permanganate Titration Glass Rod • Hydrochloric Acid –
Method • Filtration Set up (Gooch 1N -
Crucible with Suction) • Methyl Red Indicator
• Hot plate Solution
• Ammonium Oxalate
Solution
• Urea
• Dilute Sulphuric Acid –
1N
• Sodium Oxalate
• Standard Potassium
Permanganate
Solution (Potassium
permanganate,
sodium oxalate)
22 Magnesiu Clause 5.2; Sr.No.xvi) IS 3025 (Part 46):1994 • Vacuum Pump • Methyl Red Indicator Detectio
m of Table 2 i)Gravimetric Method • Filter Flasks • Hydrochloric Acid n limit
• Filter Crucibles • Ammonium Oxalate more
• Muffle Furnace • Ammonium Hydroxide than 1
• Nitric Acid, Conc mg/l
• Diammonium Hydrogen
 Phosphate
• Urea

ii) Volumetric Method • Hot Plate • Indicator Solutions

(EDTA) • Volumetric Flasks i)Patton and Reeder -
• Glasswares Reagent, Sodium
Chloride/Potassium
Chloride
ii)Murexide (Ammonium
Purpurate), Absolute
Ethylene Glycol, Sodium
Chloride
iii)Eriochrome Black T
Indicator (EBT Indicator),
Hydroxylamine
Hydrochloride,
Ethanol/Methanol
• Standard Zinc Solution
– 0.01M (Pure Zinc
Dust/Granules –
99.9% Pure;
Hydrochloric Acid)
• Buffer Solution
(Ammonium
Chloride, Ammonia,
Sodium Hydroxide-
1N)
• Standard Ethylene
 Diamine Tetra
Acetic Acid
(EDTA) Solution –
0.001M (Disodium
Ethylene Diamine
Tetra Acetate
Dihydrate, Standard
Zinc Solution)
• Triethanolamine
Solution – 10%
• Potassium Cyanide
• Hydroxlamine
Hydrochloride

• Atomic Absorption
iii)Atomic Absorption Spectrophotometer with • Hydrochloric Acid – Detectio
Spectrophotometric Method Air-Acetylene Flame or 1N and 0.1N n limit
Nitrous Oxide-Acetylene • Lanhanum Chloride less than
Flame and Hollow Cathode (Lathanum Oxide, 1 mg/l
Lamp (Magnesium) Hydrochloric Acid,
• Polyethylene Bottles Conc)
• Cesium Chloride
• Standard Magnesium
Solution (1000mg/l)
(Magnesium Oxide,
Hydrochloric Acid)

23 Sodium Clause 5.2; Sr.No.xvii) IS 3025 (Part 45):1993 • Flame Photometer (Direct • Deionized Distilled -
of Table 2 i) Flame Emission Reading OR Internal Water
Photometric Method Standard Type)
OR Atomic Absorption
Spectrophotometer (In Flame
Emission Mode)
• Glasswares
ii) Atomic Absorption • Atomic Absorption
Spectrometry Method Spectrophotometer with
Air-Acetylene Flame and
Hollow Cathode Lamp for
Sodium
• Glasswares
iii)Gravimetric Method • Beakers (20ml,
Borosilicate)
• Fritted Glass Crucible or
Porous Porcelain Crucibles
• Vacuum Pump or Aspirator
• Stock Sodium Solution
– 1mg/ml (Sodium
Chloride)
• Standard Lithium
Solution – 1mg/ml
• Sodium Chloride Detectio
• Potassium Chloride n range
• Stock Sodium Solution 0.20 to
– 1mg/ml 4.0mg/l
• Stock Potassium
Solution – 1mg/ml
• Zinc Uranyl Acetate
-
Reagent (Glacial Conc.
Acetic Acid, Uranyl
Acetate Dihydrate, Zinc
Acetate Dihydrate,
Sodium Chloride)
Ethyl Alcohol Wash
Solution (Ethyl Alcohol,
Pure Sodium Zinc Uranyl
Acetate, Sodium Chloride,
Acetic Acid, Diethyl Ether)
24 Residual Clause 5.2; IS 3025 (Part 26):1986 • Glasswares • Acetic Acid, Glacial i)Detecti
Free Sr.No.xviii) of Table 2 i)Iodometric Method • Potassium Iodide – on limit
Chlorine Crystals more
• Standard Sodium than
Thiosulphate – 0.01N 1mg/l
• Standard Potassium ii) This
Dichromate – 0.1N require
• Starch Indicator ment is
Solution not
applicab
le for
PNMW

ii) Stabilized Neutral Ortho- • Spectrophotometer • Distilled Water – Detectio

Toluidine Method
• Magnetic Stirrer Assembly
• Brown Glass Stoppered
Bottles
• Refrigerator
(Recommended)
• Buffer Stabilizer
Chlorine Demand Free n range
(Distilled Water, 0.005 to
Chlorine) 0.01mg/l
• Neutral Ortho-Toluidine
Reagent (Hydrochloric
Acid – Conc, Mercuric
Chloride, Disodium Salt
of EDTA – Dehydrated,
Ortho-Toluidine
Dihydrochloride
Reagent (Dipotassium
Hydrogen Phosphate,
 Potassium Dihydrogen
Phosphate, Di (2-Ethyl
Hexyl) Sulphosuccinate,
Diethylene Glycol
Monobutyl ether
• Potassium Iodide
Solution (Potassium
Iodide
• Sulphuric Acid Conc.
• Sodium Carbonate
• Sodium Arsenite
• Standard Chlorine
Solution (Chlorine Gas
& Distilled Water OR
Hypochlorite Solution)
• Sodium Thiosulphate
Solution – 0.025N)
25 Phenolic Clause 5.2;Sr.No.xix) IS 3025 (Part 43):1992 • Spectrophotometer • Stock Phenol Solution Detectio
Compoun of Table 2 i) 4-Aminoantipyrine Method • pH Meter (Phenol, Bromate – n limit
ds without Chloroform • Distillation Assembly (All Bromide Solution – minimu
Extraction Borosilicate Glass) with 0.1N, Hydrochloric m1
Graham Condenser Acid (Conc), Potassium mg/l
Iodide, Sodium
Thiosulphate – 0.025N,
Starch Indicator)
• Standard Phenol
Solution (1µg/ml)
• Ammonium Hydroxide-
0.5N
• Phosphate Buffer

ii) 4-Aminoantipyrine • Spectrophotometer
Method with Chloroform • Filter Funnel (Buchner
Extraction Type with Fritted Disc) OR
Filter Paper (Whatman
No40)
• pH Meter
• Separating Funnel;
• Distillation Assembly (All
Borosilicate Glass) with
Graham Codenser
Solution (Potassium
Hydrogen Phosphate,
Potassium Dihydrogen
Phosphate)
• 4 – Aminoantipyrine
• Potassium Ferricyanide
• Sodium Sulphate,
Anydrous
• Phosphoric Acid
• Phenol Stock Detectio
Solution(Phenol) n limit
• Standard Phenol 0.001mg
Solution (1µg/ml) /l
• Ammonium Hydroxide
– 0.5N
• Phosphate Buffer
Solution (Potassium
Hydrogen Phosphate,
Potassium Dihydrogen
Phosphate)
• 4-Aminoantipyrine
• Potassium Ferricyanide
• Chloroform
• Sodium Sulphate
Anhydrous
26 Mineral Clause 5.2, Sr.No.xx) IS 3025 (Part 39):1991 • Separating Funnel (1lit) • Hydrochloric Acid -
Oil of Table 2 i) Partition Gravimetric with Teflon or Equivalent • TrichlorotrifluoroEthan
Method Stopcock e
• Distillation Flask • Sodium Sulphate,
• Water Bath Anhydrous
• Filter Paper (Whatman
No.40 or Equivalent),
11cm Diameter
• Desiccator
• Analytical Balance

ii) Partition Infra-Red • Hydrochloric Acid -

• Separating Funnel (1lit)
Method with Teflon or Equivalent • Trichlori
Stopcock Trifluoroethane
• Infra-Red • Sodium Sulphate,
Spectrophotometer – Anhydrous
Double Beam, Recording • Reference Oil (Iso-
type Octane, Hexadecane,
• Cells – Infra-Red, Silica Benzene)
• Filter Paper – Whatman
No.40 or Equivalent, 11cm
Diameter
• Analytical Balance

iii)Soxhlet Extraction Method -

• Soxhlet Apparatus • Hydrochloric Acid
 • Vacuum Pump’ • Trichloro
• Buchner Funnel (12cm Trifluoroethane
Diameter) • Diatomaeous Silica
• Electric Heating Device Filter Aid Suspension
• Paper Extraction Thimble (10g/l)
• Filter Paper-Whatman
No.40 or Equivalent, 11cm
Diameter
• Muslin Cloth Discs – 11
cm Diameter
• Oven
• Water Bath
• Desiccator
• Analytical Balance
27 Anionic Clause 5.2; Sr.No.xxi) Annex K of IS 13428:1998 • pH Meter • Sodium Chloride Detectio
Surface of Table 2 • Spectrometer • Ethyl Acetate n limit
Active • Gas Stripping Apparatus (1 • Chloroform about
Agents (as lit Capacity) • Ethanol 0.05
MBAS) • Nitrogen or Air • Methanol mg/l
• Reflux Condenser • Sulphuric Acid
• Ethanolic Sodium
Hydroxide-0.1mol/lit
(Sodium Hydroxide,
Ethanol)
• Methylene Blue,
Neutral Solution
• Methylene Blue, Acidic
Solution
• Bufer Solution, pH 10
 (Sodium Hydrogen
Carbonate, Anhydrous
Sodium Carbonate)
• Phenolphthalein
Indicator, Ethanol
• Dodecyl Benzene
Sulphonic Acid Methyl
Ester (Tetrapropylene
Type), Stock Standard
Solution
28. Sulphide Clasue 5.2, Sl. No. IS 3025 (Part 29):1986 • Reaction Flask (1 lit • Zinc acetate Above 1
xxii) of Table 2 capacity) solution – 2N mg/l
i) Iodometric Method • Absorption flasks (250ml • Sulphuric Acid,
Capacity) Conc.
• Nitrogen/Carbon dioxide • Standard Iodine
gas cylinder solution – 0.025 N
Or (Potassium Iodide,
Carbon dioxide gas Iodine)
generator • Hydrochloric Acid,
Conc.
• Standard
Thiosulphate
Solution - 0.025 N
(Sodium
thiosulphate,
Sodium
Hydroxide/Chlorof
orm)
• Starch indicator
solution (Starch,
 salicylic acid,
toluene)
• Aluminium
Chloride solution –
6N
• Sodium hydroxide
– 6N
ii) Methylene blue method • Spectrophotometer (664 • N, N – dimethyl – Detectio
nm) or filter photometer p- Phenylene n limit
• Matched test tubes Diamine oxalate upto 20
• Droppers • Sulphuric Acid, mg/l
Conc. & 1:1
solution
• Ferric Chloride
• Diammonium
Hydrogen
Phosphate
• Methylene Blue
• Standard Sulphide
Solution
29. Antimony Clause 5.2 Sl. No. i) Annex G of IS • Spectrophotometer • Hydrochloric Acid -
xxiii) of Table 2 13428:1998 • Erlenmeyer Flask –6N
Spectrophotometric • Seperating Funnels (125 • Phosphoric Acid –
Method ml) with Teflon Stopcocks 3N
• Refrigerator • Rhodamine B
• Ice Bath • Antimony Standard
• Test Tubes Solution
(100 µg/ml and 1
µg/ml (pure
antimony, sulphuric
 acid)
• Benzene
• Sulphuric Acid
• Perchloric Acid
ii) IS 15303:2003 • Atomic Absorption • Metal free Water Detectio
Electrothermal Atomic Spectrometer with • Hydrochloric Acid, n limit
Absorption Conc. minimu
Spectrometric Method i) Hollow Cathode Lamp • Nitric Acid, Conc. m0.003
OR • Matrix Modifier mg/l
Electrodeless discharge Stock Solutions
lamp (Magnesium
ii) Graphite Furnace Nitrate, Nickel
iii) Readout Mechanism Nitrate, Phosphoric
• Sample Dispenser Acid, Palladium
• Vent for Fumes Nitrate, Citric Acid)
• Cooling Device • Stock Antimony
• Membrane Filter Solution (100
Apparatus (0.45µm) µg/ml)
• Chelating Resin
• Sodium hydroxide -
10 N
30. Borates Clause 5.2 & Sl. No. Annex H of IS • Spectrometer (410 – • Azomethine – H, -
xxiv) of Table 2 13428:1998 420nm) Sodium Salt
• Lab Apparatus made of • L + - Ascorbic Acid
Polypropylene/Polyethylen • Buffer Solution (pH
e/Polytetrafluoro Ethylene 5.9) [Ammonium
Acetate, Sulphuric
Acid, Phosphoric
Acid, Citric Acid,
Disodium Ethylene
 diamine –
Tetraacetic Acid
Dihydrate]
• Borate Stock
Solution -
(1mg/ml) (Boric
Acid)
• Boron Standard
Solution - 10µg/ml
• Calcium Hydroxide

31. Mercury Clause 5.2, & Sl. No. i) IS 3025 (Part 48):1994 • Atomic Absorption • Sulphuric Acid, Detectio
of Table 3 Spectrometer and conc. n limit
i) Cold Vapour Atomic Associated Equipment • Nitric Acid, Conc. minimu
Absorption (Cold Vapour Technique) • Stannous Chloride m
Spectrophotometric • Mercury Vapour • Hydrochloric Acid, 0.0002
Method Generation Assembly Conc. mg/l
• Mercury Hollow Cathode • Sodium Chloride
Lamp • Hydroxylaminesulp
• Recorder/Printer/Display hate
Meter • Potassium
Permanganate
• Potassium
Persulphate
• Stock Mercury
Solution (1mg/ml)
• Mercuric Chloride

ii) Colorimetric • Spectrophotometer • Redistilled or Detectio

Dithizone Method • Separating Funnels Deionised Distilled n limit
 (250 and 1000ml with Water minimu
PTFE Stopocks) • Stock Mercury m 0.002
• Glass wares Solution – 100 mg/l
• Whatman Filter No. 42 µg/ml (Mercuric
Chloride, Nitric
Acid – Conc.)
• Potassium
Permanganate
• Potassium
Persulphate
• Hydroxylamine
Hydrochloride
• Dithiozone Solution
– 6 µg/ml
(Dithiozone
Chloroform,
Hydrochloric Acid,
Ammonium
Hydroxide)
• Sulphuric Acid –
0.25 N
• Potassium Bromide
• Chloroform
• Disodium
Hydrogen
Phosphate
• Anhydrous
Potassium
Carbonate
• Sodium Sulphate,
 Anhydrous
32. Cadmium Clause 5.2 and Sl. No. IS 3025 (Part 41):1992 • Atomic Absorption • Hydrochloric Acid, Detectio
ii) of Table 3 i) Atomic Absorption Spectrophotometer with Conc. n range
Method (Direct) Air-Acetylene Flame • Nitric Acid, Conc. 0.05 to
• Cadmium Hollow Cathode • Stock Cadmium 2mg/l
Lamp or Multi Element Solution – 1mg/ml
Hollow Cathode Lamp for (Pure Cadmium
Use at 228.8nm Metal)

ii) Atomic Absorption • Atomic Absorption • Hydrochloric Acid, Detectio

Method (Chelation and Spectrophotometer with Conc. n range
Extraction) Air-Acetylene Flame • Nitric Acid, Conc. 0.005 to
• Cadmium Hollow Cathode • Stock Cadmium 0.2mg/l
Lamp or Multi Element Solution - 0.5
Hollow Cathode Lamp for µg/ml
Use at 228.8nm • Sodium Hydroxide
• Separating funnel • Methyl Isobutyl
Ketone (MIBK)
• Bromophenol Blue
Indicator
• Ethanol or
Isopropanol
• Pyrrolidine
• Carbon Disulphide
iii) Differential Pulse • Polarograph – Capable of • Hydrochloric Acid, Detectio
Anodic Stripping Differential Pulse Work Conc. n range
Voltammetry • Hanging Mercury Drop • Nitric Acid, Conc. 0.0001
Electrode • Hydroxylamine to
• Platinum Counter Hydrochloride 0.1mg/l
Electrode • L-Ascorbic Acid
 • Saturated calomel • Standard Cadmium
Reference Electrode Solution - 10 µg/ml
• Magnetic Stirrer Control (Pure Cadmium
Unit with Stirring Bar Metal)
• Nitrogen Gas (Cylinder) • Granular Zinc
• Scrubber Assembly for • Mercury
Nitrogen Purification • Ammonium Meta
Vanadate
33. Arsenic Cl. 5.2 and Sl.No.iii) of IS 3025(Part 37)1988 • Atomic absorption • Sodium boro -
Table 3 i) Atomic absorption spectrometer with arsenic hydride
method electrode less discharge • Sodium hydroxide
lamp • Sodium Iodide
• Atomizer • Sulphuric acid-18N
• Hydride generation system & 2.5 N
• Reaction cell for producing • Potassium
arsenic hydride persulphate
• Eye dropper or syringe • Nitric acid, conc
• Refrigerator • Perchloric acid,
• Argon or nitrogen conc
• Hydrochloric acid,
conc
• Standard arsenic
(III) solution 0.1
µg/ml.
(Arsenic trioxide)
• Standard arsenic
(V) solution 0.1
µg/ml.

(Arsenic
 pentaoxide)
• Standard organic
arsenic solution –
0.1 µg/ml.
(Dimenthyl arsenic
acid/cacodylic acid)
ii) Silver diethyl • Arsine generator and • Hydrochoric acid, Detectio
dithiocarbamate method absorption assembly conc n limit
• Spectrophotometer • Potassium Iodide minimu
(535 nm with 1 cm. cells) • Stannous chloride, m 0.001
arsenic free mg/l
• Lead acetate
• Ephedrine
• Chloroform
• Silver diethyl
dithiocarbamate
• Zinc – 20 to 30
mesh, arsenic free
• Standard arsenic
solution – 1 µg/ml
(Arsenic trioxide,
Sodium Hydroxide)
iii) Mercuric bromide • Arsine generator glass • Sulphuric acid Detectio
stain method assembly • Nitric acid, conc n limit
• Roll cotton minimu
• Lead acetate m
• Arsenic papers 0.001mg
• Mercuric bromide /l
• Ethyl
alcohol/isopropanol
 • Potassium iodide
• Arsenic free
stannous chloride
• Zinc-20 to 30
Mesh, arsenic free
• Standard arsenic
solution - 1 µg/ml
(Arsenic trioxide,
sodium hydroxide)

34. Cyanide Cl. 5.2 & Sl.No. (iv) IS 3025(Pt.27):1986 • Distillation apparatus • Sodium hydroxide Detectio
of Table 3 i) Total cyanide after consisting of boiling flask • Lead carbonate- n limit
distillation method (1lit.), thistle tube, Allihn powdered minimu
water cooled condenser, • Sulphamic acid m 0.02
gas dispersion tube, needle • Magnesium ng/l
valve, suction flask and chloride
suction pump • Sulphuric acid,
• Heating mantle conc
• Gas absorber • Acetic acid, glacial
• Ground glass st joints • Standard cyanide
• Spectrophotometer solution – 1 µg/ml
(potassium cyanide,
silver nitrate)
• Chloramine - T
• Pyridine
• Pyrazolone
• BIS – pyrazolone
• Standard silver
nitrate solution – 1
 ml = 1 mg CN
(silver nitrate)

ii) Selective electrode • Expanded – scale pH meter • Standard cyanide Detectio

method or specific Ion meter solution – 10 µg/ml n range
• Cyanide Ion selective (Potassium cyanide, 0.05 to
electrode silver nitrate) 10mg/l
• Reference electrode, • Sodium hydroxide
double junction • Potassium nitrate
• Magnetic mixer with TFE • Potassium
coated stirring Bar hydroxide
35. LEAD Cl.5.2 & SL. No.v) of IS 3025(Pt.47):1994 • Atomic absorption • Hydrochloric acid, Detectio
Table 3 i) Atomic absorption spectrophotometer with air conc n range
method (direct) acetylene flame • Nitric acid, conc. 1.0 to
• Hollow cathode lamp OR • Standard lead 10.0mg/l
Electrodeless Discharge solution – 0.1
lamp for use at 283.3 nm mg/ml
(Lead nitrate)
ii) Atomic absorption • Hydrochloric acid, Detectio
method (chelation- • Atomic absorption conc n range
extraction) spectrophotometer with air • Nitric acid, conc. 0.1 to
acetylene flame • Pyrrolidine 1.0mg/l
• Hollow cathode lamp OR • Chloroform ( with
Electrodeless Discharge • Carbon disulphide graphite
lamp for use at 283.3 nm • Sodium hydroxide system
0.001
• Bromophenol blue
mg/l)
• Ethanol or
isopropanol
• Standard lead
solution – 0.1
 mg/ml (lead nitrate)
iii) Differential pulse • Polarograph capable of • Hydrochloric acid, Detectio
anodic stripping performing differential conc. n range
voltammetry (DPASV) pulse work • Nitric acid, conc. 0.001 to
• Hanging mercury drop • Standard lead 0.1mg/l
electrode solution – 0.2 µg/ml
• Platinum counter electrode (Lead Nitrate).
• Saturated calomel • Granular zinc
reference electrode • Mercury
• Magnetic stirrer control • Ammonium meta
unit with stirring bar vandate
• Scrubber assembly for
nitrogen purification
• Nitrogen gas (cylinder)
36. Chromium Cl. 5.2 & Sl. No.vi) Annex J of IS • Atomic absorption • Deionised distilled -
of Table 3 13428:1998 spectrophotometer with water
reducing Air – acetylene • Nitric acid,
flame redistilled
• Hydrochloric acid,
redistilled
• Standard chromium
solution (chromium
oxide)
• Lanthanum chloride
• Lanthanum oxide
• Ammonium
pyrrolidine ditho
carbamate
37. Nickel Cl. 5.2 & Sl. No. Annex. L of IS • Atomic absorption • Nitric acid, conc. -
vii) of Table 3 13428:1998 spectrophotometer with • Nickel standard
 nebulizer – burner having solution-1mg/ml.
air-acetylene flame (pure nickel
• Nickel hollow cathode metal)
lamp/electrodeless • Sodium hydroxide
discharge lamp • Hydrochloric acid,
conc.
• Methyl-
isobutyleketone
(MIBK)
• Ammonium 1 –
pyrrolidine dithio
carbamate (APDC)
• Bromophenol blue
• Ethanol
38. Poly Cl.5.2 & Sl. Annex. M of • Gas chromatograph with • Silica gel, 60 – 100 -
chlorinate No.viii) of Table 3 IS13428:1998 EC detector and coupled mesh
d with printer-plotter-cum- • N-hexane-
biphenyle integrator redistilled
(PCB) • Glass chromatographic • Potassium
colum. hydroxide pellets
• Kuderna-Danish type, • Sodium hydroxide
evaporator solution – 5N
• Snyder columns • Diethyl ether,
• Syringe (5 µl) chromatography
• Heating oven grade
• Desiccator • Cotton wool
• Acetic acid, glacial,
redistilled
• Chromium trioxide,
re-crystallized.
39. Polynucle Clause 5.2 and Sl. APHA 6440 • High Performance Liquid • Reagent Water -
ar No. ix) of Table 3 i) High Chromatograph (HPLC) • Sodium
Aromatic Performance complete with gradient Thiosulphate,
Hydrocarb Liquid pumping system, reverse Granular
on Chromatographic phase column and detectors • Cyclohexane,
(HPLC) Method (UV and fluorescence) methanol, acetone,
& OR methylene chloride,
ii) Gas Gas Chromatograph (GC) pentane – Pesticide
Chromatographic complete with column and quality or
(GC) Method flame ionization detector. equivalent.
• Separating funnel (2 l) • Acetonitrile –
• Evaporative flask HPLC quality
• Three Ball Synder column • Sodium Sulphate,
• K-D Apparatus granular, anhydrous
• Water bath (60-65˚C) • Silica Gel –
100/200 mesh
• Stock standard
solution
• Std. PAHs
Solutions – (a) 100
µg/ml of
naphthalene,
acenaphthylene,
fluorine,
phneanthrene and
anthracene.
(b) 5µg/ml Benzo
(k) fluoranthene
 LIST OF TEST FACILITIES FOR MICROBIOLOGICAL TESTS OF
PACKAGED DRINKING WATER AS PER IS 14543:2004 *

Sl. Tests Clause Ref. of Referred Method of Test/ Test Equipment/Apparatus* Chemicals/Medium Remarks
No IS 14543:2004 Refer to IS
.
1. ESCHERIC Clause 5.1.1 i) IS 5887(Pt.1):1976 • General microbiological • Distilled water
HIA COLI Lab equipments: • Nutrient broth @
i) Hot air oven (capable (peptone, meat
of 180˚ C) extract, sodium
ii) Autoclave (capable of chloride)
15 psi/121ْ C) of • Nutrient Agar
suitable size depending medium @
on need (Nutrient broth,
iii) Balance for media Agar)
preparation (l.c. 0.01 g) • Mac Conkey
iv) pH meter (l.c.0.1) broth
v) Laminar air flow (Peptone, sodium
chamber OR taurocholate/Bile
inoculation salts, sodium
room/cabinet fitted chloride, lactose,
with U.V. tube light neutral red,
vi) U.V. cabinet fitted with ethanol)
U.V. lamp • Mac Conkey
vii) Hot plate for media Agar Medium
preparation (Mac Conkey
viii) Membrance filtration Broth, Agar)
assembly (including • Eosin methylene
0.45µm pore dia with blue Lactose Agar
47-50 mm diameter medium (Peptone,
sterilized filters,
 vacuum pump and Dipotassium
forceps with rounded hydrogen
tips) phosphate, Agar,
ix) Inoculating loop lactose, Eosin
x) Bunsen burner with methylene blue)
LPG cylinder • Tergitol Agar
xi) Hot plate for media medium
preparation (Proteose
xii) Water bath peptone, yeast
thermostatically extract, lactose,
controlled Agar, tergitol-7,
xiii) Microscope@ Bromothymol
xiv) Air conditioner blue)
(recommended) • TSI medium for
xv) Refrigerator H2S test @
xvi) Colony counting (Meat extract,
equipment yeast extract, peptone,
(recommended) glucose, lactose,
xvii) General glasswares sucrose, ferrous
including Petri dishes, sulphate, sodium
pipettes, flasks slides, chloride, sodium
Durham’s tubes, test thiosulphate,
tubes, both side open Agar, phenol red)
glass tubes, culture • Medium for
bottles urease test@
• Incubator capable of (Peptone, sodium
maintaining 37 ±1˚C chloride, Agar,
• Incubator capable of potassium
maintaining 44˚C @ dihydrogen
phosphate, phenol
red, glucose, urea,
 Seitz filtration
assembly)
• Medium for
indole
production@
(Peptone, sodium
chloride, strain of
bacterium
known to produce
indole)
• Kovac’s reagent
@ (p-Dimethyl-
aminobenzal
dehyde, Amyl
alcohol/Iso-amyl
alcohol, conc.
Hydrochloric
acid)
• Medium for
methyl red and
Voges-Proskauer
tests @ (Peptone,
Dipotassium
hydrogen
phosphate,
glucose)
• α-naphthol,
ethanol,
potassium
hydroxide,@
 • Simmon’s citrate
Agar @
(Sodium chloride,
magnesium
sulphate,
ammonium
dihydrogen
phosphate,
Dipotassium
hydrogen
phosphate,
sodium citrate,
Agar, BroMothymol
blue indicator)
• Peptone water
medium for
carbohydrate
fermentation test
@
(Peptone, sodium
chloride,
Andrade’s indicator,
sugar)
• Lactose@
• Gram stain @
(Methyl
violet/crystal
violet; iodine;
potassium iodide;
neutral red, acetic
 acid, ethanol)
ii) IS 15185:2002 • General microbiological • Distilled water This is a
Standard Test lab equipments • Lactose TTC reference
(as listed above) Agar method
• Water bath and/or (Lactose;
incubator thermostatically peptone; yeast
controlled (36 ±2˚ C and extract; meat extract;
44.0±0.5ْ C) Bromothymol
blue; Agar; 2,3,5-
Triphenyltetrazoli
u m chloride
(TTC); sodium
heptadecylsulphat
e (Tergitol-7);
membrane filter –
0.2 µm pore size)
• Tryptone soy agar
(TSA) (Tryptic
digest of casein;
soy peptone;
sodium chloride;
Agar)
• Tryptone broth
(Tryptic digest of
casein, L-
tryptophan,
sodium
chloride)
• Oxidase reagent
(Tetramethyl-p-
 phenylene
diamine
hydrochloride)
• Kovac’s Reagent
(p-dimethyl
aminobenzal
dehyde, amyl or
butyl alcohol,
hydrochloric
acid)
• Rapid Test • General microbiological • Distilled water This is
lab equipments • Tryptone soy optional
(as listed above) Agar(TSA) method
• Water bath and/or (Tryptic digest of
incubator thermostatically casein; soy
controlled (36 ±2ْ C and peptone; sodium
44.0±0.5ْ C). chloride; Agar)
• Ultra violet lamp wave • Tryptone bile
length 254 nm Agar (TBA)
(Low pressure mercury (Tryptone, bile
lamp) salts, Agar)
• Filter pad, dia (min.) • Indole reagent
47mm (p-
Dimethylamino
benzaldehyde,
hydrochloric
acid)

2. Coliform Cl. 5.1.2 i) IS 5401(Pt • General microbiological • Distilled water

1):2002 lab equipments; • Crystal violet
 (as listed above) neutral red bile
• Incubator (37 ± 1ْ C) lactose (VRBL)
Agar
(Peptone, yeast
extract, lactose,
sodium chloride,
bile salts, neutral
red, crystal violet,
Agar)
ii) IS 15185:2002 • General microbiological • Distilled water
Standard Test lab equipments • Lactose TTC
(as listed above) Agar
• Water bath and/or (Lactose;
incubator thermostatically peptone; yeast
controlled (36 ±2˚ C and extract; meat extract;
44.0±0.5ْ C) Bromothymol
blue; Agar; 2,3,5-
Triphenyltetrazoli
u m chloride
(TTC); sodium
heptadecylsulphat
e (Tergitol-7);
membrane filter –
0.2 µm pore size)
• Tryptone soy agar
(TSA) (Tryptic
digest of casein;
soy peptone;
sodium chloride;
Agar)
 • Oxidase reagent
(Tetramethyl-p-
phenylene
diamine
hydrochloride)
3. Sulphite Cl. 5.1.4 Annex. C of • General microbiological • Differential
reducing IS 13428:1998 lab equipments reinforced
anaerobes (as listed above) clostridial
• Volumetric pipettes – 10 medium (DRCM)
ml and 1 ml (Peptone tryptic
• Iron wire digest of meat ,
• Incurbator (37 ± 1˚C) meat extract,
• Screw cap-bottles/vials and yeast extract,
stoppers of boron silicate starch, hydrated
glass of capacities 200, 100 sodium acetate,
and 25 ml glucose, L-
• Anaerobic Jar assembly cysteine
(Recommended) hydrochloride,
sodium
hydroxide)
• Sodium sulphite
• Iron (III) citrate
4. Pseudomon Clause 5.1.5 Annex D of IS 13428 • General microbiological • Presumptive
as Lab equipments medium for
aeruginosa (as listed above) pseudomonas
• Screw capped bottles aeruginosa
• Incubator (37 ± 1˚ C) [DL asparagine, L
proline,
• Incubator (42 ± 0.5˚ C) anhydrous
@ dipotassium
 hydrogen
phosphate,
magnesium
sulphate
heptahydrate,
anhydrous
potassium
sulphate, ethanol
and cellulose acetate
or nitrate, membrane
of pore size 0.22
µm - for alternate
sterilization of
ethanol)]
• Confirmatory
medium @
(Skim milk
powder, yeast
extract bacteriological,
peptone, sodium
chloride, Agar
hexadecyltrimeth
yl ammonium
bromide/
centrimide)
• Oxidase and
catalase test
medium @
• Gelatin
liquification test
medium @
 •Acetamide @
•Starch hydrolysis
test medium @
5. Aerobic Clause 5.1.6 IS 5402:2002 • General microbiological • Plate count This
microbial lab equipments medium requireme
count (As listed above) (Tryptone, nt is not
• Incubators (21 ± I˚ C) and dehydrated yeast applicable
37 ± I˚ C) extract, for
• Colony counting anhydrous glucose, Packaged
equipment Agar) natural
mineral
water

6. Yeast and Cl. 5.1.7 IS 5403:1999 • General microbiological • Yeast extract-

mould lab equipments dextrose -
• Incubator 25 ± I˚ C chloramphenicol
Agar medium
(Yeast extract,
dextrose,
chloramphenicol/
ox ytetracycline
hydrochloride,
Agar)

* Note 1- The list does not cover the following requirements, as these parameters are got to be tested from outside approved lab :
i) Faecal streptococci and Staphylococcus aureus.
ii) Salmonella and Shigella.
iii) Vibrio cholera and V. parahaemolyticus.
Note 2 – General Microbiological Lab Equipments as listed, are common for various microbiological tests. Other additional equipments for
specific requirements are indicated against each parameter.
Note 3 – For preparation of culture media and reagents ingredients of uniform quality and chemicals of analytical reagent grade should be used.
Alternatively, commercially available media and reagents may be used provided their composition comply with those given in Indian
Standards.

Note 4 – Disposable apparatus may be accepted as an alternative to re-usable glassware.

@ The marked equipments/ chemicals and media are required for confirmatory tests of respective microbe. The confirmatory test may be
dispensed with/omitted, provided the licensee undertakes to start corrective actions based on presumptive presence of microbe .

Note : All efforts have been made to compile the list as per the respective standards exhaustively covering all the required test equipments,
apparatus and chemicals. However, in case any omission or incorrectness is noticed while referring, the same may be conveyed to
CMD immediately for suitable actions