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Criterion B: inquiring and designing
Level 1-2 Level 3-4 Level 5-6 Level 7-8
2. AIM / HYPOTHESIS
Create a research question based on the GRASP task..
4. BACKGROUND INFORMATION
Complement the background information including the following elements:
Importance of photosynthesis. Plants need this process in order to make food and
oxygen, which is needed to make respiration happen
Definition of respiration. When plants use the glucose from photosynthesis for
growth, reproduction, and repairs. This involves taking in oxygen and releasing
it as carbon dioxide
The starch test uses iodine solution, which changes from orange-yellow to blue-black
in the presence of starch. Before testing a leaf for starch, it’s necessary to remove the
green colour of chlorophyll and the waxy cuticle, so that the iodine solution can
penetrate the cells of the leaf.
5. REQUIREMENTS
ethanol kettle of boiling water leaf
water bath at 80 °C with boiling-tube rack marker pen forceps
iodine solution in dropper bottle large beaker boiling tube
glass rod Petri dish white tile
eye protection
6. SAFETY
Read the method carefully and follow the instructions.
Be careful with the stove.
1. METHOD
Testing the starch in food
Procedure:
1. Put a sample of the selected item on a plate
2. take a dropper and drop one milliliter of iodine on the sample(the color of the
iodine should be orange at this stage)
3. if the sample turns blue or black the sample contains starch
My Experiment Procedure
1. Place two seeds of the same kind. One inside, receiving no sunlight, one
outside receiving sunlight
2. Wait 2-3 days to see if the plants sprout, take a sample each.
3. Check if the samples contain starch by placing the sample inside iodine.
4. If the sample turns blue or blackit contains starch
1. Put on eye protection.
2. Pour boiling water into a large beaker.
3. Pick up the leaf by the stalk with the forceps and hold it in the water for about a minute.
4. Remove the leaf from the water with the forceps and place it in a boiling tube. Push the leaf to the bottom of
the tube with the glass rod. Label the tube with your initials.
5. Pour enough ethanol into the tube to cover the leaf. Place the tube into the tube rack in the water bath. The
ethanol will boil and remove the chlorophyll.
6. Check the leaf after a few minutes. If there is still green chlorophyll in the leaf, replace the tube for a few
more minutes.
7. When the leaf has lost all green colour, use forceps to remove the leaf from the tube and rinse it in cold
water.
8. Place the leaf on a white tile and place drops of iodine solution to cover the leaf.
9. Where there is any starch in the leaf, a blue-black colour will form.
include the described method in your own experimental methodology remember to create a way to
measure the results as well as a way to control the variables.
10. ANALYSIS
1. Explain fully why a leaf turns blue-black after using this method. What does this evidence mean?
2. A plant is left in a dark cupboard for 2 days. What colour would you expect to see after one of its
leaves is tested with iodine solution, yellow-orange or blue-black? Explain your answer.
3. Suggest why a plant should be left in a dark cupboard for 2 days before carrying out experiments on
photosynthesis.
1. CONCLUSION
Draw one conclusion for the experiment.
B. Suggest changes in the method that can improve the accuracy, or the
reliability of the experiment.
REFERENCES
Include the reference of the consulted sources, according to the teacher’s instructions.
https://www.pbslearningmedia.org/resource/tdc02.sci.life.stru.photosynth/photosynthesis/