Reference Absorbance (nm) M simiarum Absorbance (nm)

Concentration 1 2 3 Concentration 1
5 1.189 1.168 1.175 5 1.104
10 0.873 0.877 0.849 10 0.665
25 0.662 0.759 0.653 25 0.469
50 0.451 0.482 0.498 50 0.156

DPPH (Control/Blank) 1.351 DPPH (Control/Blank) 1.355

Ascorbic Acid Absorbance (nm) Ascorbic Acid Absorbance (nm)

Concentration 1 2 3 Concentration 1
5 0.714 0.735 0.718 5 0.807
10 0.632 0.647 0.649 10 0.622
25 0.361 0.368 0.362 25 0.252
50 0.107 0.104 0.105 50 0.101
Absorbance (nm) M simiarum % Inhibition
2 3 Concentration 1 2 3
1.123 1.106 5 18.52399 17.12177 18.3763838
0.783 0.754 10 50.92251 42.21402 44.3542435
0.462 0.459 25 65.38745 65.90406 66.1254613
0.186 0.195 50 88.48708 86.27306 85.6088561

Absorbance (nm) Ascorbic Acid % Inhibition

2 3 Concentration 1 2 3
0.831 0.811 5 40.44280 38.67159 40.14760 39.75400
0.634 0.641 10 54.09594 53.21033 52.69373 53.33333
0.244 0.255 25 81.40221 81.99262 81.18081 81.52522
0.104 0.105 50 92.54613 92.32472 92.25092 92.37392

M
100
f(x) = 1.127946381
90 R²
f(x)==0.8737661264
1.357732761
R² = 0.8763960743
80

70

Mean % Inhibition
60

50

40

30

20

10

0
0 10
 M simiarum
Concentration Mean % Inhibition IC50
5 18.00738
10 45.83026
19.47
25 65.80566
50 86.78967

Control (+)
Concentration Mean % Inhibition IC50
5 39.75400
10 53.33333
7.65
25 81.52522
50 92.37392

M. simiarum - Mean % Inhibition (DPPH Assay)

0
f(x) = 1.1279463815x + 41.3678238823
0 R²
f(x)==0.8737661264
1.357732761x + 23.5592539599

% Inhibition
R² = 0.8763960743
0

0
Mean % Inhi bi tion (Ta nghas )
0 Li near (Mea n % Inhi bi tion
(Tangha s))
0 Mean % Inhi bi tion (Control )
Li near (Mea n % Inhi bi tion
0 (Control ))

0
0 10 20 30 40 50 60

Concentrations (ug/mL)
 Trial 1
Concentration % Inhibition IC50
5 18.52399
10 50.92251
18.14
25 65.38745
50 88.48708

Control (+) Trial 1

Concentration % Inhibition IC50
5 40.44280
10 54.09594
7.13
25 81.40221
50 92.54613

M. simiarum - Mean % Inhibition (Replicate 1)

100
f(x) = 1.1139394694x + 42.0581319388
90 R² ==0.8777470497
f(x) 1.3356427442x + 25.7782965585
R² = 0.8491156206
80

70

60 M s i mi arum Repl i ca
% Inhibition

Li nea r (M s imi a rum

50
Control Repli cate 1
40 Li nea r (Control Repl i

30

20

10

0
0 10 20 30 40 50 60

Concentrations (µg/mL)
 Trial 2
Concentration % Inhibition IC50
5 18.37638
10 44.35424
20.44
25 66.12546
50 85.60886

Control (+) Trial 2

Concentration % Inhibition IC50
5 38.67159
10 53.21033
8.04
25 81.99262
50 92.32472

plicate 1) M simiarum - % Inhibition (Repl

100
f(x) = 1.1445137959x + 40.7982545918
90 R² = 0.8636564778
f(x) = 1.3416672942x + 23.4287220423
R² = 0.8793475154
80

70

M s i mi arum Repl i cate 1 60

% Inhibition

Li nea r (M s imi a rum Repl i cate 1)

50
Control Repli cate 1
Li nea r (Control Repl i cate 1) 40

30

20

10

0
0 10 20 30 40 50

Concentrations (ug/mL))
 Trial 3
Concentration % Inhibition
5 17.12177
10 42.21402
25 65.90406
50 86.27306

Control (+) Trial 3

Concentration % Inhibition
5 40.14760
10 52.69373
25 81.18081
50 92.25092

rum - % Inhibition (Replicate 2) M. simia

100
918 f(x) = 1.1253859477x + 41.2470818
90 R²
423 f(x)==0.8796691293
1.3958882245x + 21.4707424
R² = 0.8915933575
80

70

60

% Inhibition
Repl i cate 2
Li near (Repl i cate 2) 50
Control (+) Repl i cate 2
Li near (Control (+) Repl i cate 2) 40

30

20

10

0
40 50 60 0 10 20 30

s (ug/mL)) Concent
l3
IC50

20.44

+) Trial 3
IC50

7.78

M. simiarum - % Inhibition (Replicate 3)

1.1253859477x + 41.2470818586
.8796691293 + 21.470742449
1.3958882245x
.8915933575

Repl i cate 3
Li nea r (Repl i cate 3)
Control (+) Repl i cate 3
Li nea r (Control (+) Repl i cate 3)

0 20 30 40 50 60

Concentrations
 M simiarum Absorbance (nm)
Concentration 1 2 3
5 1.104 1.123 1.106
10 0.665 0.783 0.754
25 0.469 0.462 0.459
50 0.156 0.186 0.195

DPPH (Control/Blank) 1.355

Ascorbic Acid Absorbance (nm)

Concentration 1 2 3
5 0.807 0.831 0.811
10 0.622 0.634 0.641
25 0.252 0.244 0.255
50 0.101 0.104 0.105
 M simiarum % Inhibition
Concentration 1 2 3
5 18.52399 17.12177 18.37638 18.00738
10 50.92251 42.21402 44.35424 45.83026
25 65.38745 65.90406 66.12546 65.80566
50 88.48708 86.27306 85.60886 86.78967
P5 50.4428 38.67159 40.1476
P10 54.09594 53.21033 52.69373

Ascorbic Acid % Inhibition

Concentration 1 2 3
5 50.4428 38.67159 40.1476 43.08733
10 54.09594 53.21033 52.69373 53.33333
25 81.40221 81.99262 81.18081 81.52521
50 92.54618 92.32472 92.25092 92.37394
 M simiarum % Inhibition
Concentration 1 2 3
A5 18.52399 17.12177 18.37638 18.00738
B10 50.92251 42.21402 44.35424 45.83026
C25 65.38745 65.90406 66.12546 65.80566
D50 88.48708 86.27306 85.60886 86.78967
P5 50.4428 38.67159 40.1476 43.08733
P10 54.09594 53.21033 52.69373 53.33333
SUMMARY OUTPUT

Regression Statistics
Multiple R 0.986859
R Square 0.973891
Adjusted R 0.960836
Standard E 0.259923
Observatio 4

ANOVA
df SS MS F Significance F
Regression 1 5.04008 5.04008 74.60154 0.013141
Residual 2 0.13512 0.06756
Total 3 5.1752

Coefficients
Standard Error t Stat P-value Lower 95%Upper 95%
Lower 95.0%
Upper 95.0%
Intercept 3.874 0.217467 17.81418 0.003136 2.938314 4.809686 2.938314 4.809686
X Variable 1.004 0.116241 8.637218 0.013141 0.503855 1.504145 0.503855 1.504145
One factor ANOVA

Mean n Std. Dev

10.0 3 10.00 A
56.7 3 5.77 B
76.7 3 5.77 C
96.7 3 5.77 D
60.0 12 34.11 Total

ANOVA table
Source SS df MS F p-value
Treatment 12,400.00 3 4,133.333 82.67 2.32E-06
Error 400.00 8 50.000
Total 12,800.00 11

Post hoc analysis

p-values for pairwise t-tests
A B C D
10.0 56.7 76.7 96.7
A 10.0
B 56.7 4.05E-05
C 76.7 2.87E-06 .0085
D 96.7 3.83E-07 .0001 .0085

Tukey simultaneous comparison t-values (d.f. = 8)

A B C D
10.0 56.7 76.7 96.7
A 10.0
B 56.7 8.08
C 76.7 11.55 3.46
D 96.7 15.01 6.93 3.46

critical values for experimentwise error rate:

0.05 3.20
0.01 4.38

Comparison of Groups
120.0

100.0

80.0

60.0

40.0

20.0
80.0

60.0

40.0

20.0

0.0
A B C D
 Number of BS Dead % Lethality (%)
Concentration R1 R2 R3 Concentration R1 R2 R3
1 µg/mL 0 2 1 1 µg/mL 0 20 10
10 µg/mL 5 6 6 10 µg/mL 50 60 60
100 µg/mL 8 7 8 100 µg/mL 80 70 80
1000 µg/mL 10 10 9 1000 µg/mL 100 100 90

A B C
1 0 50 80
2 20 60 70
3 10 60 80
 % Lethality
Mean Concentration Mean % Lethality Concentrations (µg/mL)
10.00000 1 µg/mL 10 1
56.66667 10 µg/mL 57 10
76.66667 100 µg/mL 77 100
96.66667 1000 µg/mL 97 1000

D
100
100
90
Mean % Lethality LogC Probit Intercept 3.874 y=ax+c
10 0 3.72 X Variable 1 1.004 5=1.004x+3.874
57 1 5.18 x=1.122
77 2 5.74
97 3 6.88 LC50 13.228602
6.5614527