Abstracts / Maturitas 81 (2015) 191–236
Results: Results showed a low level of knowledge on breast can-
cer particularly on symptoms and risk factors of breast cancer. Only
20% of the women have ever performed breast self-examination
(BSE) on a regular basis. Clinical breast examination (CBE) was per-
formed by 15% of eligible participants and less than 10% reported
ever having at least one mammography. Multiple Linear Regres-
sion analysis showed that knowledge on breast cancer screening,
perceived benefits of BSE, confidence to do BSE were significant
predictors of breast cancer screening practices (p < 0.05).
Conclusion: This study indicates women’s beliefs and knowl-
edge play important roles for BCS. Therefore they need to
understand benefits of breast cancer screening and perform prac-
tices towards breast health care in the community.
http://dx.doi.org/10.1016/j.maturitas.2015.02.378
P238
Personal profile of the cervix cancer screening
in peri and postmenopausal gynecologist
members of the Spanish association of
menopause study. CHUSES study
Maria Cancelo Hidalgo 1,∗ , Maria Cornellana
Puigarnau 2 , Gisselle González Segura 3
1
Hospital Universitario de Guadalajara, Universidad
de Alcalá, Guadalajara, Spain
2 GINEX, Clínica Corachan, Barcelona, Spain
3 Hospital Universitario de Guadalajara,
Guadalajara, Spain
Objective: To assess the habits related to guidelines for cer-
vical cancer screening in peri and postmenopausal gynecologists
member of the Spanish Association for the Study of Menopause.
Methods: Prospective observational study. The analysis tool
used was an anonymous and self-fill out questionnaire which was
specifically designed for this purpose.
Among 114 gynecologist members of AEEM, 51 responses were
analyze (47.7%). These responses belong to gynecologist members
aged between 40 and 65 years, peri or postmenopausal.
Results: 51.1% of the gynecologist members of AEEM follow
Obstetrician and Gynecology Spanish Society guideline for cervi-
cal cancer screening. Those who do not follow that guideline, 40.4%
follow up on less cytology screening than guideline recommenda-
tions. 2.1% follow up on more cytology screening.
The result of cytology is normal in 85.1%, 4,3% of cytology are
ASCUS/AGUS, 4.3% LSIL, HSIL is reported in a 6.4% and there is not
reported any case of carcinoma of the cervix.
The proactive attitude in preventing cervical cancer is done by
95.6% of gynecologists.
Conclusions: Gynecologist members of AEEM perform in their
own cervical cancer screening, although only 50% follow Obstetri-
cian and Gynecology Spanish Society guideline.
http://dx.doi.org/10.1016/j.maturitas.2015.02.379
233
SKIN AGEING
P239
Bioinformatic identification of potential
sacrificial proteins in ageing tissues
Matiss Ozols 1 , Sarah A. Hibbert 1 , Rachel E.B.
Watson 1 , Christopher E.M. Griffiths 1 , Michael J.
Sherratt 2,∗
1 University of Manchester, Manchester, United
Kingdom
2 University of Manchester, Molecular Biochemistry,
Manchester, United Kingdom
Introduction: Age-related changes in the mechanical proper-
ties of structural extracellular matrix (ECM) proteins are associated
with clinical signs of ageing. In contrast to short-lived intracellu-
lar proteins, individual ECM proteins, and in particular elastic fibre
components, are required to function for many decades. Hence, it
has been assumed that age-related damage, mediated for example
by exposure to ultraviolet radiation (UVR) and/or reactive oxygen
species (ROS), gradually compromises protein function. However,
not all amino acid residues are susceptible to UVR/oxidation and we
have previously shown that: (i) fibrillin microfibrils and fibronectin,
which are rich in the UV-chromophore amino acid residues Cys,
Trp, and Tyr, are damaged by low doses of UVR which have no
detectable effect on the structure of chromophore-poor collagen
I and; (ii) elastic fibre associated proteins are enriched in these
chromophores compared with the entire human proteome.
Aims: To determine if UV chromophore-rich proteins are differ-
entially distributed in UV exposed and protected tissues.
Objectives: To identify key structural proteins in skin, hair and
eye and to characterise the relative abundance of Cys, Trp and Tyr
residues.
Methods: Swiss-Prot derived protein sequences (244) were
characterised using the bioinformatics tool ProtParam.
Results: Tissues which receive the most UV exposure contained
abundant chromophore-rich proteins (late cornified envelope pro-
teins in the stratum corneum, keratin associated proteins in hair,
fibrillin-1 in the papillary dermis and ␤ and ␥ lens crystallins).
Conclusions: Evolution appears to have favoured the deposition
of UV-susceptible proteins in UV-exposed tissues where they can
act as sacrificial sunscreens (rather than acting as passive targets
of UVR damage). Intriguingly, Cys, Trp and Tyr are also oxidation-
sensitive suggesting that the same proteins may act as sacrificial
antioxidants in non-UV exposed tissues.
http://dx.doi.org/10.1016/j.maturitas.2015.02.380
SLEEP
P240
Sleep disorders in postmenopausal women:
lipid peroxidation and antioxidant system
Natalya Semenova 1,∗ , Luybov Kolesnikova 1 , Irina
Madaeva 1 , Olga Berdina 2 , Elena Solodova 2 ,
Marina Darenskaya 1
1 Scientific Centre of Family Health and Human
Reproduction Problems, Department of Reproductive
Pathophysiology, Irkutsk, Russian Federation
2 Scientific Centre of Family Health and Human
Reproduction Problems, Irkutsk, Russian Federation
Introduction: Some studies have demonstrated increased
lipid peroxidation activity in climacteric women and suggested