Lampiran 1
Lampiran 1
61
Lampiran 1.(Lanjutan)
Tabel III. Hasil penetapan kadar abu tidak larut asam simplisia seledri.
62
Lampiran 1. (Lanjutan)
Tabel V. Hasil kadar senyawa larut dalam air simplisa seledri.
63
Lampiran 1. (Lanjutan)
3 Bau Khas
(gram)
1,0002 7,76
1,0017 8,43
1,0029 9,57
64
Lampiran 1. (Lanjutan)
Tabel XI. Hasil penetapan kadar abu tidak larut asam ekstrak seledri.
Berat krus
Kadar abu
Berat krus Berat krus + Kosong +
tidak larut
No kosong ekstrak hasil
asam
(W0) (g) (W1) (g) pemijaran
(%)
(W2) (g)
1 33,4082 33,5254 33,4087 0,42
2 33,4097 33,5258 33,4102 0,43
65
Lampiran 1. (Lanjutan)
Tabel XII. Hasil kadar senyawa larut dalam air ekstrak seledri.
(g)
Berat cawan
Berat cawan +
Berat cawan Kosong + Kadar senyawa
20 ml
No kosong hasil larut asam
filtrat
(W0) (g) pemijaran (%)
(W1) (g)
(W2) (g)
1 84,9192 101,0186 85,0750 4,84
2 84,9190 101,0186 85,0751 4,85
66
Lampiran 1.(Lanjutan)
6
4
5
3
2
1
Keterangan :
67
Lampiran 1.(Lanjutan)
= 0,24 cm
Tabel XIV. Hasil pengukuran Kromatografi Lapis Tipis (KLT) Ekstrak seledri.
68
Lampiran 1. (lanjutan)
Tabel XV. Data berat burung puyuh jantan selama aklimatisasi (7 Hari).
bobot burung
kelompok bobot burung puyuh
puyuh sebelum selisih
dan Hewan setelah aklimatisasi
aklimatisasi (%)
perlakuan (gram)
(gram)
1 102 110 7,8
2 105 111 5,7
3 104 110 5,8
kontrol
4 99 105 6,4
negatif
5 112 118 5,4
rata-rata 104,4 110,8 6,22
SD 4,83 4,66 0,95
1 118 125 5,9
2 108 115 6,5
3 99 108 9,1
kontrol
4 101 108 6,9
positif
5 105 113 7,6
rata-rata 106,2 113,8 7,2
SD 7,46 6,98 1,23
1 103 109 5,8
2 107 116 8,4
3 100 108 8
Dosis 25
4 105 112 6,7
mg/KgBB
5 108 113 4,6
rata-rata 104,6 111,6 6,7
SD 3,21 3,21 1,57
1 107 115 7,5
2 118 125 5,9
3 120 128 6,7
Dosis 50
4 106 110 3,8
mg/KgBB
5 110 118 7,3
rata-rata 112,2 119,2 6,24
SD 6,42 7,33 1,5
1 115 120 4,3
2 118 125 5,9
3 121 120 7,4
Dosis 100
4 110 110 4,5
mg/KgBB
5 100 115 5
rata-rata 112,8 118 5,42
SD 8,23 5,70 1,27
69
Lampiran 1. (Lanjutan)
Tabel XVI. Hasil pengaruh dosis terhadap tebal dinding pembuluh darah aorta
2.5
2 1.89 1.83
rata-rata tebal dinding
1.47 1.45
1.5
1.14
1 kontrol negatif
kontrol positif
Gambar 3. Diagram tebal dinding pembuluh darah aorta burung puyuh jantan.
70
Lampiran 1.(Lanjutan)
Tabel XVII. Tabel hasil pengukuran diameter lumen pembuluh darah aorta
Diameter lumen
kontrol kontrol dosis 25 dosis 50 dosis 100
negative positif mg/KgBB mg/kgBB mg/kgBB
Hewan (µm) (µm) (µm) (µm) (µm)
1 3,21 2,21 2,25 4,02 4,25
2 3,35 2,36 1,96 3,32 4,36
3 3,34 1,93 1,92 4,17 3,76
4 4,01 2,16 2,20 3,31 4,06
5 3,54 1,89 2,16 3,98 3,86
Rata-rata 3,49 2,11 2,098 3,76 4,1
SD 0,31 1,19 0,15 0,41 0,25
5
4.5 4.04 4.1
4 3.76
Rata-rata diameter lumen
3.5
3 kontrol negatif
2.5 2.11 2.098 kontrol positif
2
dosis 25 mg/KgBB
1.5
dosis 50 mg/KgBB
1
dosis 100 mg/KgBB
0.5
0
kontrol kontrol dosis 25 dosis 50 dosis 100
negatif positif mg/KgBB mg/KgBB mg/KgBB
Perlakuan
71
Lampiran 1. (Lanjutan)
Tabel XVIII. Hasil pengaruh dosis terhadap tingkat kerusakan sel endotel
4
3.6
3.5
3
TINGKAT KERUSAKAN
2.5
kontrol negatif
2
kontrol positif
1.4 1.4
1.5 1.2 dosis 25 mg/KgBB
1 dosis 50 mg/Kg
0.5 dosis 100 mg/KgBB
0
kontrol kontrol dosis 25 dosis 50 dosis 100
negatif positif mg/KgBB mg/Kg mg/KgBB
PERLAKUAN
72
Lampiran 1. (Lanjutan)
Tabel XIX. Data rasio berat organ jantung burung puyuh jantan
1.8
1.6 1.53 1.52
1.36 1.35
1.4 1.26
Rasio berat jantung
1.2
1 kontrol negatif
0
kontrol kontrol dosis 25 dosis 50 dosis 100
negatif positif mg/KgBB mg/KgBB mg/KgBB
Perlakuan
73
Lampiran 1. (Lanjutkan)
Tabel XX. Data rasio berat organ ginjal burung puyuh jantan.
0.9
0.8 0.74
Rasio berat organ ginjal
0.7 0.64
0.6 kontrol negatif
74
Lampiran 1. (Lanjutan)
Tabel XXI. Data rasio berat organ hati burung puyuh jantan
2.5
2.24
2 2.04
2
RASIO BERAT ORGAN HATI
0
kontrol kontrol dosis 25 dosis 50 dosis 100
negatif positif mg/KgBB mg/KgBB mg/KgBB
PERLAKUAN
75
Lampiran 1. Perhitungan
1. Susut pengeringan
W0 = 9,8800 g
W1 = 12,3877 g
W2 = 12,2960 g
(W1−W0)− (W2−W0)
SP = X 100%
(W1−W0)
= 3,66 %
2. Kadar abu total
W0 = 25,4678 g
W1 = 27,9204 g
W2 = 25,5232 g
W2−W0
Kadar abu total = X 100 %
W1−W0
25,5232 g −25,4678 g
KAT = 25,5232 g −25,4678 g x 100 %
0,0554 g
= X 100 %
2,4526g
= 2,26 %
76
Lampiran 1. (Lanjutan)
W2 − W0 100
kadar senyawa larut dalam air = x 100%
W1 − W0 20
= 19,34 %
W0 = 77,2301 g
W1 = 82,3205 g
W2 = 77,3092 g
W2 - W0 100
Kadar senyawa larut etanol= x x 100 %
W1 - W0 20
0,0791 100
= 5,0904 X X 100 %
20
= 7,76 %
77
Lampiran 1. (Lanjutan)
5. Penentuan Rendemen :
Ekstrak kental
% Rendemen = x 100 %
Sampel Kering
30,4917 g
= x 100 %
228,8 g
= 13,32 %
6. Persentase selisih berat badan burung puyuh sebelum dan sesudah aklimatisasi
𝐵𝐵 𝑠𝑒𝑠𝑢𝑑𝑎ℎ 𝑎𝑘𝑙𝑖𝑚𝑎𝑡𝑖𝑠𝑎𝑠𝑖−𝐵𝐵 𝑠𝑒𝑏𝑒𝑙𝑢𝑚 𝑎𝑘𝑙𝑖𝑚𝑎𝑡𝑖𝑠𝑎𝑠𝑖
% selisih berat badan = 𝑥 100 %
𝐵𝐵 𝑠𝑒𝑏𝑒𝑙𝑢𝑚 𝑎𝑘𝑙𝑖𝑚𝑎𝑡𝑖𝑠𝑎𝑠𝑖
Contoh :
= 7,8 %
78
Lampiran 1. (Lanjutan)
Tabel XXII. Hasil uji anova satu arah berat organ jantung burung puyuh jantan.
Descriptives
organ_jantung
N Mea Std. Std. 95% Mini Max
n Deviat Erro Confidence mu imu
ion r Interval for m m
Mean
Lower Upper
Bound Bound
KONTRO
1,53 ,08487 ,037 1,4289 1,6397 1,42 1,63
L 5
4340 72 9582 51 29 74 81
POSITIF
KONTRO
1,35 ,07934 ,035 1,2576 1,4546 1,26 1,46
L 5
6120 03 4821 06 34 47 76
NEGATIF
DOSIS 25 1,51 ,06610 ,029 1,4357 1,5999 1,44 1,58
5
mg/KgBB 7840 74 5641 57 23 80 64
DOSIS 50 1,34 ,08232 ,036 1,2430 1,4474 1,25 1,43
5
mg/KgBB 5220 51 8169 00 40 40 86
DOSIS
1,26 ,10076 ,045 1,1383 1,3885 1,12 1,37
100 5
3420 56 0637 03 37 20 87
mg/KgBB
1,40 ,13173 ,026 1,3490 1,4577 1,12 1,63
Total 25
3388 27 3465 11 65 20 81
79
Lampiran 1. (Lanjutan)
ANOVA
organ_jantung
Sum of df Mean F Sig.
Squares Square
Between
,277 4 ,069 9,960 ,000
Groups
Within Groups ,139 20 ,007
Total ,416 24
organ_jantung
Duncan
Perlakuan N Subset for alpha =
0.05
1 2
DOSIS 100
5 1,263420
mg/KgBB
DOSIS 50
5 1,345220
mg/KgBB
KONTROL
5 1,356120
NEGATIF
DOSIS 25
5 1,517840
mg/KgBB
KONTROL
5 1,534340
POSITIF
Sig. ,111 ,758
Means for groups in homogeneous subsets are
displayed.
a. Uses Harmonic Mean Sample Size = 5,000.
80
Lampiran 1. (Lanajutan)
Tabel XXIII. Uji anova satu arah berat organ hati burung puyuh jantan.
Descriptives
organ_hati
N Mean Std. Std. 95% Confidence Mini Maxi
Deviatio Error Interval for Mean mum mum
n Lower Upper
Bound Bound
kontrol 1,323 ,235543 ,1053 1,03111 1,61604 1,137 1,735
5
negatif 580 2 381 4 6 4 8
kontrol 2,247 ,322721 ,1443 1,84714 2,64857 1,932 2,727
5
positif 860 8 256 8 2 5 4
dosis 25 1,390 ,143092 ,0639 1,21234 1,56769 1,234 1,543
5
mg/KgBB 020 3 928 7 3 8 9
dosis 50 1,993 ,392193 ,1753 1,50684 2,48079 1,638 2,467
5
mg/KgBB 818 2 941 6 0 9 6
dosis 100 2,043 ,272546 ,1218 1,70512 2,38195 1,785 2,369
5
mg/KgBB 540 4 864 9 1 8 9
1,799 ,460768 ,0921 1,60956 1,98996 1,137 2,727
Total 25
764 6 537 8 0 4 4
ANOVA
organ_hati
Sum of df Mean F Sig.
Squares Square
Between
3,463 4 ,866 10,603 ,000
Groups
Within Groups 1,633 20 ,082
Total 5,095 24
81
Lampiran 1. (Lanjutan)
organ_hati
Duncan
Perlakuan N Subset for alpha =
0.05
1 2
kontrol negative 5 1,323580
dosis 25
5 1,390020
mg/KgBB
dosis 50
5 1,993818
mg/KgBB
dosis 100
5 2,043540
mg/KgBB
kontrol positif 5 2,247860
Sig. ,717 ,198
Means for groups in homogeneous subsets are
displayed.
a. Uses Harmonic Mean Sample Size = 5,000.
82
Lampiran 1. (Lanjutan)
Tabel XXIV. Uji anova satu arah berat organ ginjal burung puyuh jantan.
Descriptives
ORGAN_GINJAL
N Mean Std. Std. 95% Confidence Mini Maxi
Deviatio Error Interval for Mean mum mum
n Lower Upper
Bound Bound
KONTROL ,2409 ,236135 ,1056
5 -,052241 ,534161 ,0702 ,6295
POSITIF 60 6 031
KONTROL ,3432 ,167170 ,0747
5 ,135691 ,550829 ,2032 ,6327
NEGATIF 60 2 608
DOSIS 25 ,7395 ,174604 ,0780
5 ,522700 ,956300 ,5721 ,9530
mg/KgBB 00 6 855
DOSIS 50 ,6387 ,130327 ,0582
5 ,476917 ,800563 ,5326 ,8022
mg/KgBB 40 3 842
DOSIS 100 ,3130 ,150670 ,0673
5 ,125958 ,500122 ,1403 ,5005
mg/KgBB 40 6 819
,4551 ,256683 ,0513
Total 25 ,349146 ,561054 ,0702 ,9530
00 9 368
ANOVA
ORGAN_GINJAL
Sum of df Mean F Sig.
Squares Square
Between
,966 4 ,241 7,845 ,001
Groups
Within Groups ,616 20 ,031
Total 1,581 24
83
Lampiran 1. (Lanjutan)
ANOVA
ORGAN_GINJAL
Sum of df Mean F Sig.
Squares Square
Between
,966 4 ,241 7,845 ,001
Groups
Within Groups ,616 20 ,031
Total 1,581 24
ORGAN_GINJAL
Duncan
PERLAKUAN N Subset for alpha =
0.05
1 2
KONTROL
5 ,240960
POSITIF
DOSIS 100
5 ,313040
mg/KgBB
KONTROL
5 ,343260
NEGATIF
DOSIS 50
5 ,638740
mg/KgBB
DOSIS 25
5 ,739500
mg/KgBB
Sig. ,394 ,375
Means for groups in homogeneous subsets are
displayed.
a. Uses Harmonic Mean Sample Size = 5,000.
84
Lampiran 1. (Lanjutan)
Tabel XXV. Uji anova satu arah tebal dinding aorta burung puyuh jantan.
Descriptives
Tebaldinding
N Mea Std. Std. 95% Confidence Mini Maxi
n Deviati Error Interval for Mean mum mum
on Lower Upper
Bound Bound
kontrol 1,90 ,0204
5 ,04583 1,8431 1,9569 1,83 1,95
positif 00 9
kontrol 1,47 ,0337
5 ,07550 1,3763 1,5637 1,38 1,58
negative 00 6
dosis 25 1,82 ,0205
5 ,04604 1,7708 1,8852 1,78 1,90
mg/KgBB 80 9
dosis 50 1,45 ,0273
5 ,06107 1,3782 1,5298 1,39 1,55
mg/KgBB 40 1
dosis 100 1,14 ,0288
5 ,06442 1,0600 1,2200 1,09 1,25
mg/KgBB 00 1
1,55 ,0575
Total 25 ,28773 1,4396 1,6772 1,09 1,95
84 5
85
Lampiran 1. (Lanjutan)
ANOVA
Tebaldinding
Sum of df Mean F Sig.
Squares Square
Between
1,916 4 ,479 134,532 ,000
Groups
Within Groups ,071 20 ,004
Total 1,987 24
Tebaldinding
Duncan
Perlakuan N Subset for alpha = 0.05
1 2 3
dosis 100
5 1,1400
mg/KgBB
dosis 50
5 1,4540
mg/KgBB
kontrol negative 5 1,4700
dosis 25
5 1,8280
mg/KgBB
kontrol positif 5 1,9000
Sig. 1,000 ,676 ,071
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5,000.
86
Lampiran 1. (Lanjutan)
Tabel XXVI. Uji anova satu arah luas lumen pembuluh darah aorta burung puyuh
jantan.
Descriptives
DIAMETER
N Mea Std. Std. 95% Confidence Mini Maxi
n Deviati Error Interval for Mean mum mum
on Lower Upper
Bound Bound
KONTROL 2,110 ,0882
5 ,19736 1,8649 2,3551 1,89 2,36
POSITIF 0 6
KONTROL 3,490 ,1402
5 ,31361 3,1006 3,8794 3,21 4,01
NEGATIF 0 5
DOSIS 25 2,098 ,0663
5 ,14839 1,9137 2,2823 1,92 2,25
mg/KgBB 0 6
DOSIS 50 3,760 ,1844
5 ,41237 3,2480 4,2720 3,31 4,17
mg/KgBB 0 2
DOSIS 100 4,058 ,1131
5 ,25302 3,7438 4,3722 3,76 4,36
mg/KgBB 0 5
3,103 ,1780
Total 25 ,89025 2,7357 3,4707 1,89 4,36
2 5
87
Lampiran 1. (Lanjutan)
ANOVA
DIAMETER
Sum of df Mean F Sig.
Squares Square
Between
17,448 4 4,362 55,440 ,000
Groups
Within Groups 1,574 20 ,079
Total 19,021 24
DIAMETER
Duncan
PERLAKUAN N Subset for alpha = 0.05
1 2 3
DOSIS 25
5 2,0980
mg/KgBB
KONTROL
5 2,1100
POSITIF
KONTROL
5 3,4900
NEGATIF
DOSIS 50
5 3,7600 3,7600
mg/KgBB
DOSIS 100
5 4,0580
mg/KgBB
Sig. ,947 ,144 ,109
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5,000.
88
Lampiran 1. (Lanjutan)
Tabel XXVII. Uji anova satu arah tingkat kerusakan sel endotel aorta burung
puyuh jantan.
Descriptives
KERUSAKAN
N Mea Std. Std. 95% Confidence Mini Maxi
n Deviati Error Interval for Mean mum mum
on Lower Upper
Bound Bound
KONTROL
5 3,60 ,548 ,245 2,92 4,28 3 4
POSITIF
KONTROL
5 1,40 ,548 ,245 ,72 2,08 1 2
NEGATIF
DOSIS 25
5 3,00 1,000 ,447 1,76 4,24 2 4
mg/KgBB
dosis 50
5 1,40 ,548 ,245 ,72 2,08 1 2
mg/KgBB
DOSIS 100
5 1,20 ,447 ,200 ,64 1,76 1 2
mg/KgBB
Total 25 2,12 1,166 ,233 1,64 2,60 1 4
89
Lampiran 1. (Lanjutan)
ANOVA
KERUSAKAN
Sum of df Mean F Sig.
Squares Square
Between
24,240 4 6,060 14,429 ,000
Groups
Within Groups 8,400 20 ,420
Total 32,640 24
KERUSAKAN
Duncan
PERLAKUAN N Subset for alpha =
0.05
1 2
DOSIS 100
5 1,20
mg/KgBB
KONTROL
5 1,40
NEGATIF
dosis 50 mg/KgBB 5 1,40
DOSIS 25
5 3,00
mg/KgBB
KONTROL
5 3,60
POSITIF
Sig. ,650 ,159
Means for groups in homogeneous subsets are
displayed.
a. Uses Harmonic Mean Sample Size = 5,000.
90