DISUSUN OLEH:
NAMA : DEWI NURAKMAL
NIM : F107171011
KELOMPOK : 4 (EMPAT)
UNIVERSITAS TANJUNGPURA
PONTIANAK
2019
Abstrak
Abstract
In the Lateral Bud Growth Inhibition and Apical Shoot Dominance Practicum, the
tools used are razor blades, spatula, glass preparation, cover glass, and
microscope. While the ingredients used are mung bean sprouts (Phaseolus
radiatus), petri dishes, and IAA paste 400 ppm. The method of work done is
provided by 6 days old mung bean sprouts in a petri dish. This germination is
carried out in a dark room at 25 ° C. Then cut the shoots of 2 sprouts just below
the first pair of leaves with a razor blade and the remaining tip is given IAA paste.
The other 2 sprouts are cut and left, and the remaining 2 sprouts are left as a
control. Each sprout is given the treatment that has been determined. Then the pot
is stored in a dark room. Then after 5 days the IAA paste is cleaned and replaced
with a new one. After 14 days, the total plant length was measured and observed
under a microscope cross section of the control rod and the end of the treated
stem. The result is that there are differences in the length of the three treatments,
the control treatment is higher than the treatment cut and cut + smeared with IAA.
The IAA treatment was cut and smeared shorter than the two previous treatments.
Sprouts that are not cut off and stored in a dark place will further activate the
auxin work because auxin works optimally in the absence of sunlight. So that the
sprouts will grow faster. Shoots of green bean sprouts (Phaseolus radiatus) that
are cut will stop the work of auxin. When green bean sprouts (Phaseolus radiatus)
are smeared with IAA it will activate the auxin action again, because IAA is a
substitute for auxin.
Keywords: Buds, apical, lateral, auxin, IAA
BAB II
METODOLOGI
Alat yang digunakan adalah pisau silet, sudip, gelas preparat, gelas penutup, dan
mikroskop. Sedangkan bahan yang digunakan yaitu kecambah kacang hijau
(Phaseolus radiatus), cawan petri, dan pasta IAA 400 ppm.
Cara kerja yang dilakukan adalah disediakan 6 kecambah kacang hijau berumur 5
hari dalam cawan petri. Perkecambahan ini dilakukan di ruang gelap pada suhu
25°C. Kemudian dipotong pucuk 2 kecambah tepat di bawah pasangan daun
pertama dengan pisau silet dan ujung sisanya diberi pasta IAA 2 kecambah
lainnya di potong dan di biarkan, dan 2 kecambah sisa dibiarkan sebagai kontrol.
Setiap kecambah diberi perlakuan yang sudah ditentukan. Selanjutnya pot
disimpan di ruang gelap. Kemudian setelah 5 hari pasta IAA dibersihkan dan
diganti dengan yang baru. Setelah 14 hari, diukur panjang tanaman seutuhnya dan
diamati di bawah mikroskop penampang melintang batang kontrol dan ujung
batang yang mendapat perlakuan.
BAB III
A. Hasil Pengamatan
Panjang Panjang
Pertambahan
No. Perlakuan Sebelum Sesudah Keterangan
Panjang
Perlakuan Perlakuan
Kerja auksin bertambah,
1 Kontrol 11 cm 29 cm 18 cm
memiliki tunas lateral
Kerja auksin bertambah,
2 Potong 14 cm 25 cm 11 cm
memiliki tunas lateral
Potong + Kerja auksin bertambah,
3 13 cm 22 cm 9 cm
IAA memiliki tunas lateral
B. Pembahasan
KESIMPULAN
SARAN
Pada saat melakukan pengukuran lebih hati-hati dan teliti agar tidak
terjadi kekeliruan.