DAFTAR ISI

Halaman
Halaman Pengesahan ........................................................................................................ 1
Daftar Isi ........................................................................................................................... 2
Ringkasan .......................................................................................................................... 5
Bab 1 Pendahuluan ......................................................................................................... 4
1.1 Latar Belakang ............................................................................................................ 4
1.2 Rumusan Masalah ....................................................................................................... 6
1.3 Tujuan Penelitian ........................................................................................................ 6
1.4 Manfaat Penelitian ...................................................................................................... 6

Bab 2 Tinjauan Pustaka ................................................................................................. 8

2.1 Penyembuhan Luka ..................................................................................................... 8
2.2 Penyembuhan luka pasca pencabutan gigi ................................................................ 10
2.3 Angiogenesis ............................................................................................................. 10
2.4 Protein Penanda / Marker pada proses Inflamasi ...................................................... 12
2.4.1 Interleukin-6 (IL-6) ................................................................................................ 13
2.5 Penanda / Marker Proses Osteogenesis .................................................................... 13
2.5.1 Bone Sialoprotein (BSP) ....................................................................................... 16
2.6 Kulit Buah manggis ................................................................................................. 17
2.7 Methyl-Thiazol-Tetrazolium atau MTT Assay ....................................................... 18
2.8 Wound Healing Assay ……………………….………………………..…................18
2.9 Polymerase Chain Reaction (PCR) …....................................................................... 19
2.10. Real-Time PCR ................………………………………………………………. 20
2.11 Osteoblastic Cell Line MC3T3-E1 ….…………………………………………… 20
2.12 Lipopolisakarida (LPS) ...................................……………………………….….. 21

Bab 3 Metode Penelitian ............................................................................................... 23

3.1 Jenis Penelitian.......................................................................................................... 23
3.2 Rancangan Penelitian ................................................................................................ 23
3.3 Sampel dan Besar Sampel ......................................................................................... 23
3.3.1 Jenis Sampel........................................................................................................... 23
3.3.2 Besar Sampel ......................................................................................................... 23
3.4 Variabel Penelitian .................................................................................................... 24
3.5 Definisi Operasional ................................................................................................. 25
3.5.1 Osteoblastic Cell Lini MC3T3-E1 ......................................................................... 25
3.5.2 Lipopolisakarida ................................................................................................... 25
3.5.3 Ekstak Kulit manggis ............................................................................................. 25
3.5.4 Ekspresi marker penanda Inflamasi ....................................................................... 25
3.5.4.1Interleukin 6 (IL-6) ............................................................................................. 25
3.5.5 Ekspresi marker penanda Osteogenesis ................................................................. 26
3.5.5.1 Bone Sialoprotein (BPS) ..................................................................................... 26
3.6 Tahapan Penelitian .................................................................................................... 26
3.7 Waktu dan Tempat Penelitian ................................................................................... 27
3.8 Alat dan Bahan Penelitian ........................................................................................ 27
3.8.1 Alat Penelitian ....................................................................................................... 27
3.8.2 Bahan Penelitian ................................................................................................... 28
3.9 Cara Kerja …............................................................................................................. 29
3.9.1 Pembuatan Ekstrak Kulit Manggis ..................................................................... 29
3.9.2 Kultur sel ................................................................................................................ 29
3.9.2.1 Osteoblastic cells line MC3T3-E1.......................................................................30
3.9.2.2 Pemberian Ekstrak Kulit Buah Manggis Pada Kultur Osteoblastic cells line

MC3T3-E1...........................................................................................................30

3.10 Polymerase Chain Reaction (PCR) …....................................................................30

3.10.1 Ekstraksi RNA …………………………………………..……………………...30
3.10.2 Pengukuran kadar dan kemurnian RNA................................................................31
3.10.3 Perancangan PCR primer IL-6 dan BPS .............................................................. 31
3.10.4 Visualisasi hasil PCR dengan menggunakan elektroforesis pada gel .................. 32
3.11 PCR Real Time…..………………………………………………………............. 34
3.12 MTT Assay ............................................................................................................. 35
3.13 Analisis Data .......................................................................................................... 36
3.14 Luaran per tahun .................................................................................................... 36
3.16 Indikator Capaian ................................................................................................... 36
Bab 4 Road Map Penelitian ………………………………………………………….37