Code R7312305 1

III B.Tech I Semester (R07) Regular & Supplementary Examinations, November 2010
PLANT BIOTECHNOLOGY
(Biotechnology)
Time: 3 hours Max Marks: 80
Answer any FIVE questions
All questions carry equal marks
?????

1. (a) What are the compounds involved in the tissue culture media composition.
(b) Explain about embryogenesis.

2. (a) Describe about androneogenesis and their development.

(b) Explain the gynogenesis and their development.

3. (a) What are the secondary metabolites produced by plant cell culture.
(b) Give the applications of secondary metabolites.

4. Explain in detail:

(a) Vectors DNA transfer by physical method.

(b) Vectors DNA transfer by chemical method.
(c) Viral Vectors applications in plant biotechnology.

5. (a) Explain the biotic stress.

(b) Explain the bacterial and fungal diseases.

6. (a) Explain the glyphosate resistance and mechanism of glyphosate.

(b) Define the strategies of glyphosate resistance.

7. (a) What are therapeutic proteins? Explain the production of therapeutic proteins in plants.
(b) Give the applications of therapeutic proteins.

8. (a) Write the metabolic pathway of primary metabolism process.

(b) Explain the primary metabolites.

?????
Code R7312305 2
III B.Tech I Semester (R07) Regular & Supplementary Examinations, November 2010
PLANT BIOTECHNOLOGY
(Biotechnology)
Time: 3 hours Max Marks: 80
Answer any FIVE questions
All questions carry equal marks
?????

1. Explain the following:

(a) Protoplast isolation culture and fusion.

(b) Organogenesis.
(c) Somatic embryogenesis.

2. Describe about applications of

(a) Production haploids

(b) Cryopreservation
(c) Somaclonal variations.

3. (a) Describe the production of secondary metabolites.

(b) Explain the process of enhancing production of secondary metabolites.

4. What do you mean by vector less DNA transfer and describe in detail vectors less gene transfer.

5. (a) What are the methods involved in viral resistance to the plant? Explain.
(b) What are the advantages and disadvantages of viral resistance?

6. (a) Explain abiotic tolerance to water deficit stress.

(b) What are the major environmental impacts on herbicide resistant crops?

7. What do you mean by plant bodies? Explain the production of plant bodies with neat labeled
diagram and its applications in plant biotechnology.

8. Explain the metabolic engineering of plant primary and secondary metabolites.

?????
Code R7312305 3
III B.Tech I Semester (R07) Regular & Supplementary Examinations, November 2010
PLANT BIOTECHNOLOGY
(Biotechnology)
Time: 3 hours Max Marks: 80
Answer any FIVE questions
All questions carry equal marks
?????

1. (a) Explain different types of plant tissue culture techniques.

(b) What are the factors affecting plant tissue culture?

2. (a) What is the basis of somaclonal variations?

(b) Write about isolation of somaclonal variations in the case of without invitro and with in
vitro selections.

3. Describe different types of bioreactor systems and models for mass cultivation of plant cells.

4. (a) Explain the agro bacterium mediated gene transfer.

(b) Explain the agro bacterium based vectors.

5. (a) Write about pest resistance and genes of different micro organisms used for pest resistance.
(b) Explain the Bt Toxin gene and its mode of action of cry proteins.
(c) Write the advantages and disadvantages of transgenic plant with Bt gene.

6. Explain abiotic stress on plant growth and explain the different factors involved in abiotic
stress.

7. What do you mean by edible vaccines and explain the production of vaccines in plants and its
applications?

8. Explain the metabolic engineering of the following:

(a) Carbohydrate metabolism in plants.

(b) Lipids metabolism in plants.

?????
Code R7312305 4
III B.Tech I Semester (R07) Regular & Supplementary Examinations, November 2010
PLANT BIOTECHNOLOGY
(Biotechnology)
Time: 3 hours Max Marks: 80
Answer any FIVE questions
All questions carry equal marks
?????

1. (a) Describe in detail of initiation and maintenance of callus and cell suspension culture tech-
niques.
(b) Write the preparation of tissue culture media.

2. (a) Write about cryopreservation and its mechanism.

(b) Explain the technique of cryopreservation.

3. (a) Explain the technology of plant cell culture for production of chemicals.
(b) Write the advantages and disadvantages of production of secondary metabolites.

4. (a) Define plant viruses and vectors.

(b) Write the limitations of viral vector gene transfer.

5. (a) What are the pathogens involved in disease resistance?

(b) Explain the bacterial and fungal diseases.

6. Explain the abiotic stress tolerance and explain in detail about drought, temperature and salts.

7. (a) Write the applications of industrial enzymes in plant biotechnology.

(b) Explain the production of industrial enzymes in plants.

8. Explain the secondary metabolites and explain the pathway of secondary metabolism and their
applications.

?????