Ilife Catalog

e mail : info@ilifediscoveries.com www.ilifediscoveries.
com
Building Stronger R
& D Relations in
Bio & Pharma Sciences
through complete Technology Solutions
Complete and Customized
Biological Services and Solutions
As a unique discovery and contract research organization, iLife Discoveries provides
molecular biology and preclinical drug discovery services to pharmaceutical, biotechnology,
academia and Public & Private Institutions on an outsourced basis. With the advent of
recent scientific and technological advances, iLife Discoveries has broadened its services
& solutions portfolio in order to accelerate researchers in all phases of the early-stage
discovery process and support them in streamlining their custom discovery and
development programs. iLife also specializes in complex experimental designs, protocol
development and execution. iLife has also established itself as a leader in expertise
development through high end technology trainings in pharma and life sciences sector.
Global Presence
iLife is Headquartered in the Silicon Valley, USA, with its Global Discovery Center
establised in the Delhi NCR region of India. The global visibility and India advantage thus
converge to create attractive features of cost benefit, technology expertise, adherence to
national and international Intellectual Property rules and rapid access to qualified human
resources. With a wealth of in-house scientific expertise, world class R&D facility, and
the most advanced technologies, iLife is strategically possitioned to deliver Quality, Speed,
Competitive Costs, Flexibility, Custom Solutions and is the Contract Research Organization
of choice for the Life Sciences & Pharma Industry.
The Team
The iLife team is committed to excellence. The highly skilled scientific staff is comprised
of individuals having a breadth of expertise including preclinical, academic research, and
CRO research experience. Our research group and technical specialists have a combined
40+ years of global experience working in multiple areas of research in infectious diseases,
oncology, molecular diagnostics, bioagriculture, metabolic disorders to name a few. The
management team and staff offer their expertise in working with you to assure the success
of the design and implementation of your project, within the timelines. iLife’s commitment
to quality is second to none, piloted by an experienced leader in the life sciences domain.
Our Capabilities
Life sciences research and discovery process is highly complex, lengthy, and expensive,
yet essential for delivering safe and effective systems for improvising human healthcare.
Even though the process is reasonably well-defined, each program is fundamentally
different and shaped by many unique factors. To help our customers navigate this process,
iLife offers a comprehensive range of contract research solutions and services that span
the entire discovery continuum and can be tailored to specific research conditions. Our
portfolio covers a broad array of basic research in the molecular biology workflow and
preclinical research. We undertake research projects in cell biology, genomics, proteomics,
bioinformatics, molecular diagnostics, High throughput screening and drug discovery
studies which are designed to accelerate the pace and increase the productivity of your
discovery and development programs.
Our offerings are continually expanding based on the changing needs of our customers
and our team of experts understands and ultimately assists our customers by providing
services to span all stages of research discovery while complying with international
scientific regulations.
Consulting – Developing Solutions Together
A long-standing experience in molecular and cell biology forms the basis of iLife’s exceptional
consulting services. This service addresses the needs of research and business partners
operating in the fields of biotech, pharma and diagnostics. During the development and
standardization of a new application or protocols or instrument platform, our customers have
to make important decisions. With our flexible consulting service, we effectively support our
partners in achieving their goals. Our consultations are project specific process with a set of
recommendations for our customers. These help to define practical work flows which then
can be outsourced to iLife Discoveries. Let us sit down, join forces and develop solutions
together.
The Facility
Doing what’s best for our clients means furnishing a full range of equipment backed by a
smart, experienced team to get them through the difficult and challenging research and
discovery process. The Global Discovery Center is an ultra modern, 30,000 sq. ft., state-of-
the-art facility and houses India’s largest CLASS-10000 clean room laboratory cluster
exclusively dedicated to biological research and development for our customers. A US FDA
209E Code compliant facility is equipped with latest high throughput part 21 CFR compliant
technologies including Agilent Microarrays, Automated Liquid Handling workstations,
sequencers, HPLC, microfluidics based technologies for analysis, QPCR, OCTET RED for
biomolecular interactions, Flow Cytometers, and others.
Collaboration
Having several active and ongoing collaborative research programs, iLife continues to seek
academic, government and commercial research partnerships in HTS, diagnostics, food testing,
forensics, agriculture and drug discovery. We are interested in collaborating to pursue
government funding for joint efforts including grant applications and competitive contracts.
For gaining its own intellectual property, iLife funds several in-house drug discovery program
for the purpose of developing commercially relevant intellectual property. Contact our team
for more information on establishing a collaboration.
The iLife Advantage

• Partnership in R&D from concept to operational strategies- end to end solutions
• Accommodating to ever demanding challanges of faster deliverables
• Personalized attention at every stage of the work dedicated teams for individual project
• Fostering frequent, open communication during all phases of a study
• Reproducible results and innovative data analysis to help accelerate decision making
• Managing the integrity, compliance, timeliness, format, and confidentiality of your data
• Time tested logistics and client support worldwide- customized modules for sample storage
and shipment through our India - US operations
• Digital documentation of individual projects on proprietary iLife LIMS platform
• Strict adherence to international, business practices, regulatory compliances, transparency
and accountability
India’s Largest US FDA 209E code compliant class 10000 Clean
Room Laboratory Cluster dedicated exclusively for
R&D IN PHARMA & LIFE SCIENCES
World class, ultra modern laboratory
exclusively dedicated to R & D
Customized Solutions
Every research lab has its own requirement. We have the tailor-made solution.
With our customized solutions, iLife aims to offer the highest standards of services through custom- made
solutions. We actively manage our customer’s projects while constantly seeking value-added innovations and
opportunities. Our capabilities are based on scientific expertise and experience, state-of-the-art instruments
and technologies, and the ability to discover and develop solutions. Each customer has a dedicated scientific
manager who focuses on individual objectives and needs. For all our solutions, our focus remains on
accomplishing your research goals while maintaining confidentiality and business ethics.
Training and Expertise Development

To be successful, it is essential for organizations to put their research team through periodic training programs,
which can enhance their knowledge and competitiveness in several critical areas. At iLife Discoveries, Training
and Expertise Development remains an integral part for scientific human recourse development. iLife delivers
value-added customized technical, management and soft skills training solutions for the global pharma and
biotech sector. We design and deliver practical training solutions for developing specific expertise to raise the
competence of our client’s employees to best–in–industry. Our emphasis is on the practical application of
knowledge and technical expertise essential for effective working in the pharma, biotech and associated life
sciences industries. We achieve this through balanced and highly stimulating learning modules with tremendous
planning, practical applications and continuous up-gradation of our own technical expertise and resources.
If you would like to have a discussion about a customized training program or develop a training strategy for
your scientific, sales or management group, please contact the Training Co-ordinator at iLife.
“Collaboration with external partners has today
become an essential part of every research group in
Academia or Industry working in Life Sciences
domain. At iLife Discoveries, we are proud of our
ability to establish open and collaborative
partnerships to share ideas, expertise and
capabilities to make both parties successful.”
Anand Gupta
Founder and CEO
“Seamless and real time access to multitudes of

rapidly evolving technologies and discovery
platforms have essentially become the fundamental
“mantra” of success to global academic and
corporate research involved in therapeutics and
diagnostics. With a very focused approach in the
technology intensive pre-clinical “in-vitro” biology
space, iLife Discoveries has carved its own footprint
in providing simpler solutions to complex research
problems to its expanding client list. Our rapid access
to technology combined with our ability to fast-track
their translation to innovative research problems
make us unique in the early discovery support
sector.”
Dr.
Anil Mavila
President and Technology Head
Corporate Floor at iLife Discoveries

Customized Support
Because we’re as passionate about your success as you are.
Your support needs are diverse as your research and our services go far beyond traditional
notions of customer support. We will be glad to assist you with particular issues, of course –
quickly and efficiently in every step of the way. From our friendly telephone representatives to
our unmatched team of experienced technical experts and Business Coordinators, we promise
to deliver an excellent level of support you deserve from a world class organization.
If you need help choosing the right services, our experts are available to discuss your needs
and provide recommendations. Email us your contact information and area of research and
one of our experts will contact you.
If you need immediate assistance,

Please call us at +1- 408 - 907- 6645 or +91- 124 – 4779800.
Innovating Life Through Science
Areas of Expertise
Animal Research
• Identification of economic trait loci (ETL) such as
growth, meat yield etc.
• QTL mapping and marker assisted selection of the
population
• cDNA microarrays to characterize gene expression
patterns of experimental animals
• Real time PCR based assays for animal genotyping,
gene expression profiling, copy number variation
Service Information
• Identification of genetic pathways important for

animal production and health.
• Identification of SNP related to disease traits
• Genotyping of animals populations based on
molecular markers
• cDNA, EST and SSH library construction
• Construction of genomic libraries
• Genome sequencing
• Transcriptome analysis using Next Gen Sequencing
• SureSelect target enrichment for Next Gen
Sequencing
• Annotation of livestock genome using human and
mouse genome
• Identifying functional element in animal genome
using information of other genome
• DNA modifications in diseased and cloned animals
• Methylation microarray
• CGH microarray
• ChIP-on-chip array
• Recombinant protein production of animal origin
• Deciphering animal development through
proteomics
• High throughput protein profiling of all animal
samples(Antibody microarray, 2-D gel
electrophoresis, off-gel fractionation)
• Identification of species specific peptides/proteins
by MALDI-TOF
• Study of Biomolecular interaction of animal proteins
(enzymes, hormones, transcription factor etc.) using
OCTET technology
• Allele Mining
• Microsatellite genotyping of livestock population
• Study of Tumor Metastasis
• Meat Quality testing using genomics and proteomics
• DNA Barcoding
• Establishment of primary cell lines
For Technical or Customer Assistance, (Asia) Call +91 124 - 477 9800
10 (US, Canada & Europe) Call +1 408 - 907 6645
Agrobiotechnology
• Identification of desirable traits in plants
Areas of Expertise
• Identification of QTL of economic traits in plants
• Genetic and physical mapping of QTL
• Construction of large insert genomic libraries
• SureSelect target enrichment for Next Gen Sequencing
• Genome sequencing strategies using Next Gen Sequencing
• Transcriptome analysis (ESTs, Microarrays, Next Gen sequencing)
• Gene expression in response to environmental stimuli (Stress – biotic & abiotic), treatment, development stage
• Gene expression profiling by microarray
• Gene expression by Real time PCR
• Epigenomics - DNA and protein modifications
• CGH microarray
• DNA sequence analysis and data handling
• Finding genes related to stress conditions
• Finding regulatory features in gene structure by comparative genome analysis
Service Information
• Regulation of gene expression
• miRNA profiling by microarray
• Plant Pathogen (Fungal) genomics and proteomics
• Marker Screening and Discovery
• Integrative description of plant function (Productivity, Quality, Sustainability)
• GMO Testing by molecular methods
• Metabolite Analysis by HPLC
• Protein/peptide identification by off gel fractionation followed by MALDI - TOF
• Seed quality and genotype testing by molecular methods
• Testing the authenticity of Basmati rice samples by molecular methods
• Rapid wheat varietal identification
• Comprehensive identification of proteins, their isoforms, and their prevalence in each tissue
• Study of Biomolecular interaction of plant proteins (enzymes, hormones, transcription factor etc.) using OCTET technology
• Real time PCR based assays for plant genotyping, gene expression profiling, copy number variation
• Microsatellite genotyping of plant population
• Allele Mining
(US, Canada & Europe) Call +1 408 - 907 6645 11
Microbiology and Pathobiology
Areas of Expertise
Service Information
• Species level identification using molecular techniques

• Genus level identification using molecular techniques
• Comparative analysis using molecular fingerprinting techniques
• Pathogen detection using molecular techniques
• Food Testing of microbial contamination using Real Time PCR
• Establishing evolutionary relationships by sequence analysis such as 16S
• Microbial diversity studies
• Detection of bacteria and other organisms poluting air, water, soil, and food
• Microbial identification of environmental samples
• Phylogenetic analysis
• Comparison phylogenomics of pathogenic bacteria by microarray analysis
• Toxicity pathway focused gene expression profiling
Molecular Diagnostics
• Real Time PCR based detection of HIV, HPV, H1N1, Malaria, Hepatitis, TB
• Cancer diagnostic through mutation analysis
• Cancer diagnostic through gene expression profiling
• Bacterial and viral load detection by Real Time PCR
• Pathogen detection by molecular methods
• Real Time PCR/ PCR based detection of organisms for which microscopy gives false positive results e.g. T.vaginalis, N.gonorrhoeae.
• Molecular detection of intracellular pathogens such as M.genitalium.
• Identification of species such as Chlamydia sp.,Neisseria sp. where conventional method is less sensitive
• Molecular identification of species in which Subtyping is mandatory e.g. HSV, HPV, HCV
Oncology
• Gene expression in response to disease stage, disease
Areas of Expertise
progression (Benign & Malignant)
• Comparative Gene expression profiling in cancerous
and normal tissue by Microarray & Real Time PCR
• Identification of regulation of gene expression in
different cancers
• miRNA profiling by microarray
• Biomarker Discovery, Identification , Characterization
and Validation for different cancers
• Identification of Epigenomics modification associated
with different cancers
• CGH microarray
• Transcriptome analysis in cancer cells (Next Gen
Service Information
Sequencing)
• Proteomics analysis in cancer cells
• Protein Profiling by 2D Gel Electrophoresis
• High throughput Protein Profiling by Antibody
microarray
• Pathway focused Protein Profiling by Antibody
microarray
• Reverse-Phase Protein Microarray
• Protein Identification by Mass Spectrometry &
N-terminal Sequencing
• Identifying tumor associated antigens
• Profiling of disease state by antibody microarray
• Time-course studies to identify different stages of
cancers
• Identification altered genes or biochemical pathways
associated with particular cancer
• To identify new molecular classes of cancer (class
discovery)
• To predict diagnosis and classification of unknown
cancer samples (class prediction)
• Study of biomolecular interaction of cancerous protein
using Biolayer Interoferometry
Food and Nutrition Science

• Profiling and characterization of dietary and other proteins
• Biomarker discovery for the nutritional status
• Proteome analysis in solving major nutrition-associated problems in
humans and animals, such as obesity, diabetes, cardiovascular
disease, cancer, aging, and intrauterine fetal retardation.
• Microbial contamination testing of food using Real Time PCR
• HPLC based food analysis
• Detection, identification and characterization of food borne-pathogens
• GMO testing
Drug Discovery
• SAR studies and initial pharcodynamics analysis • GPCR and kinase profiling
Areas of Expertise
• High throughput screening for desired pathways/disease • Antibody arrays

indication/bioactivity • Gene and Transfection services
• Basic ADME-TOX • Biomolecular interaction studies and small molecule screening
• Co-Culture platforms for microbial pathogenesis tests. • in silico drug designing
• Design and development of Assay systems for sub-genomic • Customized discovery systems using mammalian yeast and
RNA interference (antiviral drug discovery) bacterial systems with multiple Promoter-Reporter
• FRET systems for biomolecular interactions. combinations
• Expression, characterization and protein modification studies
Service Information
Infectious Diseases Clinical Proteomics

• Diagnosis and monitoring of infectious diseases by molecular
• High throughput Protein Profiling of clinical samples by Antibody
methods
microarray
• Detection of pathogens
• Pathway focused Protein Profiling of clinical samples by
• Sequencing of microbial pathogen genome
Antibody microarray
• Cloning of disease susceptibility genes
• Reverse-Phase protein Microarray for validation of antibody
• High throughput screening of disease for pathogens by Real
microarray results
Time PCR
• Discovery, qualification and verification of Protein Biomarkers
• Discovery, qualification and validation of Protein Biomarkers
in infectious samples
in infectious samples
• Identification of protein related to disease by 2-D gel
• Real Time PCR/PCR based detection of un-culturable or
electrophoresis and MALDI - TOF
fastidious microorganisms
• Molecular diagnostic of infectious diseases such as HIV, HCV,
TB, HPV, HINI, CMV etc.
COSMODEx: Cosmeceutical Discovery using

Microarray based Expression Profiling Systems
Areas of Expertise
Specific Microarray based design and development of Animal free research systems for multifaceted testing of cosmetological products
can be accomplished under this program. Briefly, we develop and utilize novel cell lines representing epithelial keratinocytes, ectocervical
vaginal cells, buccal cavity cells, melanocytes and their corresponding tumor derived cell populations to study metabolism and kinetics of
small molecules and candidates for cosmeceutical applications at their molecular level. Using a proprietary technology, stable cell lines
are developed and their wild type (control) profile is characterized using advanced genomic tools. Panels thus created will be specific for
hypersensitivity reactions, toxicity responding genes, cellular apoptosis genes, aging and senescence pathways related genes and
metabolite converting enzyme panels, cytochrome p450 family members etc. These panels are used as reference standards for customized
research programs for identifying Cosmeceutical molecules with regards to their activity and toxicity. Additionally, these customized
chips will enable the researcher to resolve the time dependent responses of Cosmeceutical and therapeutic molecules.
Application of t-TRAP Technology for Determining Food Safety

A comprehensive and innovative method for identifying time dependent and detrimental microbial pathogens or cross contamination of
food, meat and marine products.
Service Information
It has been well established that emerging economic threats associated with the safety of food and edible materials are indeed a concern
to nations that depend largely on exports from the rest of the world. Added to the complexities is the increasing threats to global food
security by engineered or man made threats such as large scale sabotage food or edible materials.
Major food pathogens like multiple species of Clostridium, Listeria, Bacillus, Yersinia, Vibrio, Botulism, Brainerd Diarrhea, Cholera,
Shigellosis etc. Specific signature sequence will be used for making specific and customized chip for targeted food products such as
Kosher meat, marine food products etc.
PRIME-C : Pharmaceutically relevant Repository of Intelligent

Mammalian Cells
CyTOSENSE : Cell repositories Sensitized to Microbial and Mycoplasma contamination
PMCI Collection : Population specific mammalian cell line repository for drug discovery containing primary & transformed cell lines
developed through rational in-sourcing & Genetic Engineering for multipanel reporter expression systems
Service Information
Every research lab has its own requirement. We

have the tailor-made solution.
Contents
GENOMICS
DNA Extraction & Purification 20 DNA Microarrays 35
RNA Extraction & Purification 20 Transcriptome Analysis/ Gene
Plasmid DNA Purification 20 Expression Analysis 36
Agilent 2100 Bioanalyzer- Technical Note 21 microRNA profiling 37
DNA Analysis 21 Location Analysis (Discover DNA Methylation
DNA Application 21 patterns) 38
Restriction digest analysis 22 Chip-on-chip Analysis 39
PCR product analysis 22 Copy Number Analysis (Comparative
Service Information
Gene expression analysis 22 Genomic Hybridization (CGH)) 40
Food analysis 22 Copy Number Variation (CNV) 41
GMO detection 22 Custom microarray development 42
Oncology 22 Real Time PCR Assays 43
Diagnostic research 22 Absolute Quantification by qPCR 44
Forensic testing 22 Relative/Comparative Quantitation 44
RNA Analysis 22 Multiplex Real Time Assay 45
RNA Application 22 Applications 45
Analysis of total RNA 23 Validation of Microarray data 45
Analysis of mRNA 23 Molecular Diagnostics 45
Analysis of Cy5-labeled samples 23 Infectious diseases 45
Analysis of T7-RNA transcripts 23 Metabolic Disorders 46
Analysis of Small RNA 23 Child and Women health 46
Restriction Fragment length Polymorphism Primer Efficiency Determination 46
(RFLP) Analysis 24 DNA/RNA copy number assessment 46
DNA Fingerprinting 25 Environmental testing using labeled probe 47
Customized PCR Assays 26 Microbial Diagnostics 47
Primer Design, Synthesis & Validation 26 Food and Agricultural 47
PCR Analysis 26 Screening of GMOs 47
Cloning 27 SNP Analysis Using Real Time PCR 47
Cloning of DNA for sequencing 27 Splice Variant Detection Using Real time PCR 47
Cloning of DNA for expression of protein 28 Cancer Diagnostics using Real Time PCR 48
Cloning and Sequencing of Up-stream/ Real Time PCR in Forensic Science 48
Down-stream Regions from Known Sequence 28 Next Gen Sequencing 49
Cloning of STRs/ Microsatellite Markers 29 Whole Genome Sequencing 49
Full-length cDNA Cloning Services 29 Whole Transcriptome Analysis 49
Library Construction 30 ChIP sequencing 50
Genomic library construction 30 Micro RNA Analysis 50
cDNA library construction 31 SureSelect Target Enrichment Systems 51
Site Directed Mutagenesis 32 Gene Synthesis 52
Gene Sequencing 33 Bacterial Identification 53
Ready to Run sequencing 33
Single Pass 33
Fragment Analysis 34
Microsatellite Analysis 34
SNP Detection 34
Gene Walking 34
Contents
PROTEOMICS & CELL BIOLOGY BIOINFORMATICS
Protein Extraction & Purification 56
Protein Analysis 56 Introduction and Applications 74
Protein Application 56 Genomics 74
Protein Expression 56 Comparative genomics 74
Protein Purification 56 Structural genomics or structural bioinformatics 74
Antibody Analysis 56 Functional genomics 74
Food Analysis 56 Proteomics 74
Protein - Others 56 Pharmacogenomics 74
Protein Expression & Purification 57 Pharmacogenetics 74
Customized protein expression for Cheminformatics 74
E. coli, yeast and mammalian cells 57 Biomedical informatics / Medical informatics 74
Epigenetic expression of proteins under inducible Computational chemistry 74
promoters 58 In silico drug designing 75
Small Scale protein purification for proof of Agroinformatics / Agricultural informatics 75
concept studies 58 Systems biology 75
Microarray Data Analysis 76
Service Information
Customized protein purification platforms for

crystallography studies 59 Single & Two Color Gene Expression Analysis 77
Protein Profiling 60 Exon Expression & Exon Splicing Data Analysis 78
Two-Dimensional Gel Electrophoresis, Copy Number Analysis 79
followed by MALDI-TOF/TOF 60 Association Data Analysis 79
Offgel Fractionation 60 miRNA Analysis 80
Antibody Microarrays 61 Custom Chip Design and Analysis 80
Pathway focussed Protein Profiling 63 RT-PCR Data Analysis 81
Immunological assays 64 Comparative Genomics 82
ELISA Assay 64 Biological Systems Modeling 82
ELISPOT Assay 64 Sequence Analysis 82
Biomolecular Interactions Assays 65 Evolutionary Studies 82
Monitoring Antibody Concentration 65 Gene Annotation 82
Small Molecule Detection 65 Primer Designing 83
Kinetic Profiling of Immunotherapeutics 65 Prediction of Protein Structure 84
Concentration Measurements based on Protein-Protein Interaction Studies 84
Binding Rates 65 Peptide Library Synthesis 84
Protein Quantitation in Bioprocessing 66 Antibody Microarray data analysis 85
Kinetic Characterization Data 66 Shipment Guidelines 86
Detection of anti-drug Antibodies(ADA) 67 Terms & Conditions 87
Screen for Drug Inhibitors 67 Ordering Information 87
Antibody Production 68 High-end Technology Training 88
Monoclonal antibodies 68
Polyclonal antibodies 68
Peptide Synthesis 69
Custom peptide synthesis 69
Metabolite analysis 69
HPLC & LC-MS Services 69
FOOD & AGRICULTURE 69
DRUG & PHARMA 69
WATER Analysis 70
MALDI ToF/ToF Services 70
Peptide Mass Fingerprint (PMF) 70
MS/MS peptide fragmentation 70
Mass Analysis- by MALDI ToF for
proteins/peptides 70
De novo sequencing of proteins/ peptides 70
Customized Cell Biology Services 71
Adenovirus Services 71
Generation of Stably Expressing Cell Lines 71
Novel Cell Lines 71
GENOMICS
Service Information
Genomics
Know Your Genes...
Nucleic Acid Purification
DNA Extraction & Purification Plasmid DNA Purification

Extraction of DNA with high purity is a necessity for many down- Extraction of Plasmid DNA with high purity is a necessity for many
stream applications. DNA extraction & purification services for small downstream applications. Plasmid DNA extraction & Purification
and large-scale projects is available. Intact, high-quality genomic services for small and large-scale projects is available. Intact, high
GENOMICS
DNA (gDNA) is routinely extracted from various sources including quality Plasmid DNA is routinely extracted for applications like Gene
tissue (fresh and preserved), blood, saliva, other body fluids, cell Therapy & multiplex PCR, real-time quantitative PCR, DNA cloning,
cultures, plants,and forensics samples. DNA southern blot hybridization, DNA transfection, DNA
sequencing, SNPs analysis etc.
Service Information
RNA Extraction & Purification

Ordering Information
Extraction of RNA with high purity & integrity is a necessity for
much downstream application. Due to the omnipresence of RNases, Service No. Description
and the instability of RNA, integrity checks and sample quantitation GNE001 DNA Purification from Tissue
are essential steps before any RNA dependent application. RNA GNE002 DNA Purification from Blood
GNE003 DNA Purification from Cells
extraction & purification services for small and large-scale projects GNE004 RNA Purification from Tissue
are available. Industry standard of RNA is routinely extracted from GNE005 RNA Purification from Blood
GNE006 RNA Purification from Cells
various sources including tissue (fresh and preserved), blood, saliva, GNE007 Plasmid DNA Purification
other body fluids, cell cultures, plants, and forensics samples. GNE008 Customized Nucleic Acid Purification
For all prices contact : customersupport@ilifediscoveries.com
Other Related Services

Service Page No.
DNA Analysis 21
Plasmid Analysis 21
RNA Analysis 22
RFLP 24
AFLP 24
DNA Fingerprinting 25
Customized PCR Assays 26
Cloning 27
Library Construction 30
Site Directed Mutagenesis 32
Gene Sequencing 33
DNA Microarrays 35
Next Gen Sequencing 49
High-end Technology Training 88
Nucleic Acid Analysis on Agilent 2100 Bioanalyzer
Quantification of Biomolecules such as DNA, RNA and protein is an essential, integral and fundamental step for any life science research.
It is based on Lab-on-a-Chip technology which enables downscaling and integration of several experimental steps into one process, in
combination with software which automates data analysis and data archiving and generates a complete QC report with quantitation
estimate, virtual gel image and electropherogram.
GENOMICS
The Lab-on-a-Chip Approach
Service Information
The Chip technology uses microfluidics whose behavior, precise control and manipulation of fluids that are geometrically constrained to a
small, typically sub-millimeter, scale allows quantification of biomolecules with a minimum volume of 1ul. The sample moves through the
microchannels from the sample well and it is injected to the separation channel. The sample components are electrophoretically separated
and are detected by their fluorescence and translated into gel like images (bands) and electropherograms (peaks).
Agilent 2100 Bioanalyzer – Technical Note

This technology utilizes a network of channels and wells that are etched onto glass or polymer chips to build mini-labs. Pressure or
electrokinetic forces move pico liter volumes in finely controlled manner through the channels. Lab-on-a-Chip enables sample handling,
mixing, dilution, electrophoresis and chromatographic separation, staining and detection on single integrated systems. The 16-pin electrode
enables the detection of samples which connected to HV sources.
Applications for a Lab-on-a-Chip Technology
DNA Analysis
Agilent 2100 Bioanalyzer is used which is a microfluidics based platform for sizing, quantification
and quality control of DNA. Different kits allows to automatically size and quantitate PCR
fragments and restriction digests accurately and reproducibly
DNA Application
The chip technology can perform variety of applications in the life science research such as
sizing and quantitation of DNA fragments like PCR product purity, Multiplex PCR Applications,
GMO Testing, Pathogen Detection, Genotyping Applications (Duplication/Deletion, Allele
frequency), Cancer diagnostics etc...:
Restriction digest analysis Ordering Information

Sizing range exemplified by the separation of Adenovirus 2/Dra I
Detection of single base mutations Service No. Description
GDA001 Sizing range exemplified by the
PCR product analysis separation of Adenovirus 2/Dra I
GENOMICS
Separation of different mixtures of PCR products GDA002 Detection of single base mutations
GDA003 Separation of different mixtures of PCR products
Determination of PCR product impurity GDA004 Determination of PCR product impurity
Multiplex PCR analysis of bacteria in chicken GDA005 Multiplex PCR analysis of bacteria in chicken
GDA006 mRNA expression study by
Gene expression analysis comparative multiplex PCR
GDA007 Standardized end-point RT-PCR
mRNA expression study by comparative multiplex PCR GDA008 Co-amplification and response curves
Standardized end-point RT-PCR GDA009 Competitive PCR
Co-amplification and response curves GDA010 Development of meat specific assays
Competitive PCR GDA011 Sea food authencity testing
GDA012 Fish species identification by RFLP
GDA013 Strawberry and Raspberry food differentiation.
Food analysis GDA014 Basmati Rice authencity testing
Development of meat specific assays GDA015 Development of a multiplex assay for soya
Sea food authencity testing GDA016 DNA stability during food processing
GDA017 GMO detection by nested multiplex PCR
Fish species identification by RFLP GDA018 Tumor cell detection from carcinoma patient blood
Service Information
Strawberry and Raspberry food differentiation. GDA019 SNP analysis in cancer related P16 gene
GDA020 K-ras gene SNP detection
GMO detection GDA021 METH-2 downregulation in lung carcinomas
GDA022 Label-free analysis of microsatellite
Basmati Rice authencity testing instability in carcinoma
Development of a multiplex assay for soya GDA023 Genotyping of H. pylori
DNA stability during food processing GDA024 Duplications and deletions in genomic DNA
GMO detection by nested multiplex PCR GDA025 Optimization of PCR on mtDNA
GDA026 Customized DNA Analysis
Oncology
Tumor cell detection from carcinoma patient blood For all prices contact : customersupport@ilifediscoveries.com
SNP analysis in cancer related P16 gene
K-ras gene SNP detection RNA Analysis
METH-2 downregulation in lung carcinomas Agilent 2100 Bioanalyzer is used which is a microfluidics based
Label-free analysis of microsatellite instability in carcinoma platform for Industry standard of RNA quality control. RNA Integrity
Number (RIN), a quantitation estimate, and ribosomal ratios of the
Diagnostic research
total RNA sample will be provided. For mRNA samples the
Genotyping of H. pylori
percentage of ribosomal impurities is calculated.
Duplications and deletions in genomic DNA
Forensic testing RNA Application

Optimization of PCR on mtDNA This technology is the industry standard for RNA sample QC and
the Bioanalyzer Expert software generates the RNA Integrity
Note: We offer Customized solutions for nucleic acid Number (RIN), a quantitation estimate, and will calculate ribosomal
applications. Please contact technical support for further ratios of the total RNA sample. RIN provides a numerical
assistance. assessment of the integrity of RNA—independent of concentration,
instrument, or analyst—giving the scientist a high degree of
confidence in the repeatability of the experiment. It can be used for
the analysis of total RNA, small RNA, messenger RNA in nanogram
and picogram concentration range.
Analysis of total RNA Cell Fluorescence Analysis

RNA integrity and documentation of RIN number
Reproducibility of quantitation The Agilent Bioanalyzer is used for cell assays for analysis of cell
Detection of low levels of RNA fluorescence parameters for flow cytometric studies. It works on
RNA integrity for picogram level detection the principle of Pressure driven flow which is used to move cells in
GENOMICS
Genomic DNA contamination a controlled manner through the microchannels which are
hydrodynamically focused to a portion of a channel by a side stream
Analysis of mRNA of buffer and cells pass the fluorescence detector in a single file
RNA integrity and each event is monitored in a histogram or a dot plot.
Ribosomal RNA contamination in mRNA samples
It can be used for many applications such as Apoptosis,
Analysis of Cy5-labeled samples
Analysis of cRNA with and without dye in gel matrix Transfection efficiency monitoring, protein expression monitoring
Optimization of labeling reactions and gene silencing.
cRNA fragmentation
Analysis of T7-RNA transcripts

Size estimation
Service Information
Analysis of Small RNA
Note: We offer Customized solutions for nucleic acid applications. Figure: Flow cytometry on a chip. Two Color detection and a dot plot
Please contact technical support for further assistance. view for easy data evaluation.
Service No. Description
GRA001 RNA integrity and Documentation of RIN number
GRA002 Reproducibility of quantitation
GRA003 Detection of low levels of RNA
GRA004 RNA integrity for pico gram level detection
GRA005 Genomic DNA contamination
Figure : Electropherogram of RNA analysis GRA006 RNA integrity
GRA007 Ribosomal RNA contamination in mRNA samples
GRA008 Analysis of cRNA with and without dye in gel matrix
Protein Analysis GRA009 Optimization of labeling reactions
GRA010 cRNA fragmentation
A specific chip design allows the analysis of upto 10 protein samples GRA011 Size estimation
in the 5-250KDa size range with the use of different kits. A variety GRA012 Analysis of small RNA
of different samples for can be used such as column fractions, GRA012 Customized RNA Analysis
purified proteins, antibodies and cell lysate.
Figure : Direct Comparison of samples run on SDS PAGE with silver staining
and on Agilent Bioanalyzer. Concentrations are given per lane.
Restriction Fragment Length Polymorphism (RFLP) Analysis

RFLP refers to a difference between two or more samples of DNA 7500 and DNA 12000 kits
homologous DNA molecules arising from differing locations of Agilent DNA 7500 and DNA 12000 kits are designed for the sizing
restriction sites. and quantitation of double-stranded DNA fragments from 100 to
GENOMICS
7500 base pairs (DNA 7500) and 100 to 12000 bp (DNA 12000)
RFLP Analysis services are offered for the following respectively. These kits can be used to analyze, for example, PCR
and RT-PCR products as well as restriction digests.
applications:
• Genome mapping
• Cloning
• Localization of genes for genetic disorders
• Determination of risk for disease
• Genetic fingerprinting
• Characterization of genetic diversity
• Breeding patterns in animal populations
And many more……
Service Information
Figure : Electropherogram of RFLP on Agilent 2100 Bioanalyzer
Fragment Analysis for RFLP

Agilent 2100 Bioanalyzer is used which is a microfluidics based
platform for sizing, quantification and quality control of DNA.
Different kits allows to automatically size and quantitate restriction

digests accurately and reproducibly.
DNA 1000 Kit Figure : RFLP representation on Agilent 2100 Bioanalyzer

It can be used for sizing and quantitation of double-stranded DNA
fragments from 25 to 1000 base pairs. It can be used for the following
applications:
• RFLP analysis
• Heteroduplex analysis using mismatch cleavage
enzymes
GRA001 RFLP with agarose gel analysis
GRA002 RFLP with Bioanalyzer analysis
GRA003 RFLP with Sequencing analysis
DNA Fingerprinting
Fingerprints are able to determine whether two DNA samples are
from the same person, related people, or non-related people. Small
numbers of sequences of DNA that are known to vary among
GENOMICS
individuals are analyzed to get a certain probability of a match.
Applications:
• Paternity and maternity testing
• Criminal Identification and Forensics
• Personal Identification
Service Information
Fragment Analysis Ordering Information
platform for sizing, quantification and quality control of DNA.
GDF001 DNA fingerprinting, fragment sizing by
agarose gel and fragment analysis by sequencing
Different kits allows to automatically size and quantitate PCR GDF002 DNA fingerprinting, fragment sizing with
fragments and restriction digests accurately and reproducibly. bioanalyzer and fragment analysis by sequencing
DNA 1000 Kit For all prices contact : customersupport@ilifediscoveries.com

It can be used for the following applications:
• Analysis of PCR and RT-PCR products
• RFLP analyses
DNA 7500 and DNA 12000 kits

Agilent DNA 7500 and DNA 12000 kits are designed for the sizing
and quantitation of double-stranded DNA fragments from 100 to
7500 base pairs (DNA 7500) and 100 to 12000 bp (DNA 12000)
respectively. These kits can be used to analyze, PCR and RT-
PCR products as well as restriction digests.
Custom PCR Assays PCR Analysis

Industry standard customized PCR Assays services are provided Microfluidics technology of Agilent 2100 Bioanalyzer is used for
which includes primer designing, synthesis, standardization & sizing, quantification and quality control of DNA.
optimization of PCR. Detection & analysis of the PCR product is
GENOMICS
done on High-end Lab-on- Chip technology. Different kits allows to automatically size and quantitate restriction
digests accurately and reproducibly:
Custom PCR Assays services are offered by iLIFE for the
following applications:
• Amplification & Quantification of DNA
• Selective DNA isolation for genetic Fingerprinting, generating
hybridization probes for Southern or northern hyb. and DNA
Cloning, DNA sequencing etc.
• Diagnosis of diseases
• Species identification
• Multiplex-PCR for analysis of microsatellites and SNPs etc.
And many more……
Sample Submission Requirement:

Service Information
Primer Design, Synthesis & Validation

• DNA, RNA, cDNA, tissue, cells, bacterial culture
• 0.5µg - 1 µg of RNA for cDNA Synthesis
• Primer Sequence in case of Primer synthesis and designing
Gene sequence • Complete thermal profile of primers
• Standardised primers in case customer is giving the primers.
• Optimal concentration of primers minimum 500nM
Oligonucleotide Design
(Primer pair designing using
software)
Synthesis of Forward and

reverse primer (25-100nM
Concentration)
Optimization of Primers GPR001 Primer Designing, Synthesis and Validation
GCP001 CustomPCRAssay
(Thermal profile,
Non-specificity)

In-silico Validation Service Page No.
Cloning 27
Gene Sequencing 33
Primer Designing 83
Cloning
Cloning of DNA for Sequencing
Sequencing of PCR products using terminal primers (used for
amplification) would usually generate incomplete sequence data,
as it does not read the terminal 50 -100 bases on both ends. iLife
GENOMICS
Discoveries offers the service for cloning and sequencing of PCR
products in our standard vector that would enable the scientists to
acquire complete and analysed sequence data of their PCR
products.
Custom cloning service includes designing and synthesis of primer,

PCR and ligation with the vector.
You can give us cells, genomic DNA, RNA and PCR product. This
will be followed by verification of recombinant plasmid by restriction
mapping. Derived clone can be further used for:
• Generation of mutants
• Subcloning
Service Information
Note: Beyond 1 kb sequence service, each 1 kb will be charged
extra as sample.
Deliverables:
1. Clone DNA (up to 5 µg)
2. Sequence data (bi-directional, raw data)
3. Final analysed sequence data
4. Project Report
Precautions and limitations:

If providing PCR product directly:
1. The band of interest should be the most prominent product within Ordering Information
the PCR sample.
2. The samples should not have excessive smearing. Service No. Description
3. Please indicate whether the PCR product has a higher GC – GCL007 Cloning of DNA of < 1 kb with Primers provided
content
GCL008 Cloning of DNA of 1 kb with Primers provided
GCL018 Cloning DNA of < 1 kb including Primer
designing and synthesis
GCL019 Cloning DNA of 1 kb including Primer
Cloning
Cloning of DNA for Expression of Protein
iLife can assist in cloning any gene in any vector for expression of Ordering Information
the protein product. Based on the requirement, the derived protein
can have: Service No. Description
GENOMICS
• His Tag GCL029 Cloning of DNA of < 1 kb for Expression of

• Maltose binding protein domain Proteins with Primers Provided
GCL030 Cloning of DNA of 1 kb for Expression of
• FLAG Proteins with Primers Provided
The tag can even be removed on your demand. Proteins with Primers Provided
Proteins with Primers Provided
Custom cloning services will include designing and synthesis of GCL040 Cloning DNA of < 1 kb for Expression of
primers, PCR, restriction digestion and ligation with the vector. Proteins including Primer Designing and Synthesis
GCL041 Cloning DNA of 1 kb for Expression of Proteins
including Primer Designing and Synthesis
You can give us cells, RNA, PCR product and Plasmid (in case of GCL042 Cloning DNA of 2 kb for Expression of Proteins
subcloning). This will be followed by verification of recombinant including Primer Designing and Synthesis
plasmid by restriction mapping. GCL043 Cloning DNA of 3 kb for Expression of Proteins
including Primer Designing and Synthesis
Service Information
Note: Beyond 1 kb sequence service, each 1 kb will be charged as For all prices contact : customersupport@ilifediscoveries.com
extra sample.
Deliverables : Cloning and Sequencing of Up-stream/

1. Clone DNA (up to 5 µg) Down-stream Regions from Known Sequence
2. Sequence data (bi-directional, raw data)
3. Final analysed sequence data The Regulatory Elements of any gene are required for gene
4. Project Report regulation, expression and m-RNA stability studies. Therefore the
Cloning and sequencing of these regions is of great importance.
Precautions and limitations : Promoter, enhancer or 5’-untranslated region is present in up-stream
If PCR product is provided: sequences on the other hand 3’-untranslated region, terminator
1. The band of interest should be the most prominent product within sequences etc. are present at down-stream region.
the PCR sample.
2. The samples should not have excessive smearing. This service will include :
3. Please indicate whether the PCR product has a higher • Cloning of up to 2 kb of up-stream or downstream sequences
GC – content from known sequence data
• Confirmed bi-directional sequence data
• Putative regulatory regions indicated
Deliverables :
1. Cloned DNA containing up to 2 kb of up-stream/down-stream
sequence
2. Bi-directional sequence data (raw data as well as analyzed and
aligned data) with the putative regulatory regions indicated
3. Project report
GCL051 Cloning and Sequencing of Up-stream/
Down-stream Regions from Known
Sequence with Primers provided
Cloning
Cloning of STRs/ Microsatellite Markers This service will include:
• Cloning into any commercially available cloning and/ or
Genotyping is a technology that can distinguish one sample from expression vector
another. The technology takes advantage of the Short Tandem • Cloning and sequencing of full-length cDNA irrespective of the
Repeats (STRs) or Microsatellites that occur dispersed through out gene size and the number of introns present.
GENOMICS
the genomes. The STRs are more often than not polymorphic in • Fast and reliable service
nature and the number of repeats occurring in each STR locus
varies within samples. STRs that are linked/segregate with traits Deliverables:
are used for Market Assisted Selection (MAS) in breeding programs. 1. Full-length cDNA into a cloning vector
Cloning and characterization of STRs that might demonstrate 2. Bi-directional sequence data (both raw data and analysed data)
polymorphism within very similar samples are important for DNA 3. Project report
fingerprinting/ genotyping purposes.
This service will include :

• Development of 10 independent STR clones, starting from
samples or genomic DNA (minimum guaranteed)
• Sequencing of the clones, demonstrating STRs
• STR specific primers would be designed (optional)
Service Information
• Standardization of STR specific “PCR/multiplex PCR” conditions
as well as screening samples for polymorphism are also
undertaken (optional)
Deliverables :
1. 10 clones with STRs/ minimum
2. Sequence data and electropherogram
3. Final report
Ordering Information Ordering Information
Service No. Description Service No. Description

GCL055 Cloning of Full-length cDNA of 1 kb with Primers provided
GCL053 Cloning of STRs/ Microsatellite Markers
with Primers provided
GCL054 Cloning of STRs/ Microsatellite Markers
GCL065 Cloning of Full-length cDNA of 1 kb including Primer
including Primer designing and synthesis
GCL066 Cloning of Full-length cDNA of 2 kb including
Primer designing and synthesis
For all prices contact : customersupport@ilifediscoveries.com GCL067 Cloning of Full-length cDNA of 3 kb
including Primer designing and synthesis
Full-length cDNA Cloning Services Other Related Services

Service Page No.
Cloning and sequencing of full-length cDNA allows scientists to :
DNA Extraction & Purification 20
1. Analyse the intron/ exon junctions; RNA Extraction & Purification 20
2. Deduce the amino acid sequence coded by the gene; DNA Analysis 21
3. Study the up-stream/ down-stream regulatory regions; RNA Analysis 22
4. Express the gene for protein expression studies etc. Customized PCR Assays 26
Gene Sequencing 33
Protein Extraction & Purification 56
Cloning of full-length cDNA can be achieved from any of the Protein Expression & Purification 57
following information: Protein Profiling 60
• Partial/ full-length cDNA sequence data Immunological Assays (ELISA & ELISPOT) 64
Biomolecular Interaction Assays 65
• Genomic clone sequence data Antibody Production 68
• Amino acid sequence data Peptide Synthesis 69
• cDNA/ genomic DNA libraries HPLC & LC-MS Services 69
Primer Designing 83
Library construction
Genomic Library Construction
GENOMICS
Service Information
Genomic DNA libraries are commonly used when one wants to Deliverables:
preserve the entire genome of an organism. The library thus formed 1. Library delivered on plates, filters or glycerol stock
will help the scientist to study the entire genome, including large 2. Project Report
numbers of non-coding and regulatory regions from the genome.
Custom Genomic DNA library construction service

will include :
• Extraction of genomic DNA from a variety of samples
• Generation of phage- or plasmid-based DNA libraries from the Ordering Information
size fractionated restriction digested DNA
• Directional cloning of DNA fragments into any vector Service No. Description
• Production of a library with at least 10-6 cfu GLG005 Genomic DNA Library Synthesis
• Library amplification to generate higher titer library
• Verification of library quality by randomly picking clones and
determining insert sizes by restriction digest For all prices contact : customersupport@ilifediscoveries.com
• Verification of library complexity by DNA sequencing the inserts
from a subset of randomly picked clones (Optional)
Library construction
cDNA Library Construction
GENOMICS
Service Information
cDNA libraries are commonly used when one wants to reproduce Deliverables:
only the coding regions of the eukaryotic genomes, and the amount 1. Library delivered on plates, filters or glycerol stock
of information is reduced to remove the large numbers of non-coding 2. Project Report
and regulatory regions from the library.
Custom cDNA library construction service will

include:
• Extraction of Total RNA from a variety of samples
• Generation of phage- or plasmid-based DNA libraries from the
size fractionated cDNA
• Directional cloning of DNA fragments into any vector Service No. Description
• Production of a library with at least 10-6 cfu
GLG006 cDNA Library Synthesis
• Library amplification to generate higher titer library
• Verification of library quality by randomly picking clones and
determining insert sizes by restriction digest
• Verification of library complexity by DNA sequencing the inserts
from a subset of randomly picked clones (Optional)
Site Directed Mutagenesis
iLife offers a complete mutagenesis service for creating single
multiple point mutations, deletions, insertions or random mutations
into any gene of your interest.
Site Directed Mutagenesis helps in :

GENOMICS
• Study of active sites, structure - function relationships, nucleic

acid - protein interactions etc.
• Construction of fusion proteins and tagged proteins
• Construction of knockout constructs
• Generation of alternative splice forms
• Test of functional elements (promoter regions, terminator
sequences etc.)
Custom Site Directed Mutagenesis service will include :

• Design and synthesis of mutagenesis primers
• PCR amplification
• Confirmation of desired mutations by DNA sequencing
• Confirmation of undesired mutations by DNA sequencing
• Subcloning modified gene into fresh vector not subjected to the
Service Information
mutagenesis procedure or subcloning into another vector

(Optional)
• Verification of vector sequence after mutagenesis by DNA
sequencing (Optional)
• Delivery of purified plasmid DNA construct
Deliverables :
• 4 ug lyophilized destination vector containing the gene
• Sequence chromatograms covering your gene (electronic)
• Construct map for the plasmid (electronic)
• Project Report
GSM001 Plasmid <7kb, single point mutation
GSM002 Plasmid <7kb, multiple point mutations
GSM003 Plasmid >7kb, single point mutation
GSM004 Plasmid >7kb, multiple point mutations
Gene Sequencing
Ready to Run Sequencing Samples - Candidate gene PCR product/ whole genomic DNA/
Cell culture plates/ Cloned PCR Product.
Ready to run sequencing service is provided for the following:
SNP discovery
Targeted re-sequencing approach for accurate polymorphism
GENOMICS
• PCR Products identification with Sanger sequencing.
• Plasmid
• BACs High quality sequence reads are provided. Long sequences are
provided with maximum accuracy using long capillaries.
Sample required is sequence ready purified amplicons & result is
provided in the form of long read length, high quality sequence
data. Ordering Information
GSP001 Single Pass sequencing from Culture plates
(Using Universal Primers)
GSP002 Single Pass sequencing from gDNA (Using Universal Primers)
GSP003 Single Pass sequencing from Plasmid (Using Universal Primers)
GSP004 Single Pass sequencing from RNA (Using Universal Primers)
GSP005 Single Pass sequencing from cDNA (Using Universal Primers)
Service Information
GSP006 Single Pass sequeincing from cloned vectors
GSP07 Single Pass sequencing from BAC/YAC (Using Universal Primers)
GSP08 Single Pass sequencing from non-purified PCR amplicons
GSP09 Single Pass sequencing from purified PCR amplicons
GSP010 Single Pass sequencing from Culture plates
(Using Custom Primers)
GSP011 Single Pass sequencing from gDNA (Using Custom Primers)
GSP012 Single Pass sequencing from Plasmid (Using Custom Primers)
GSP013 Single Pass sequencing from RNA (Using Custom Primers)
GSP014 Single Pass sequencing from cDNA (Using Custom Primers)
GSP015 Single Pass sequeincing from cloned vectors
Ordering Information GSP016 Single Pass sequencing from BAC/YAC (Using Custom Primers)
GSP017 Single Pass sequencing from non-purified PCR amplicons
Service No. Description GSP018 Single Pass sequencing from purified PCR amplicons
GRR001 Ready to run gDNA amplicons (Using Custom Primers)
GRR002 Ready to run Plasmid amplicons
GRR003 Ready to run BAC amplicons For all prices contact : customersupport@ilifediscoveries.com

Single Pass
Service Page No.
Single-pass sequencing services are provided for the following DNA Extraction & Purification 20
applications: RNA Extraction & Purification 20
Plasmid DNA Purification 20
de-novo Sequencing Customized PCR Assays 26
Cloning 27
For initial/primary sequencing of genetic content of organisms. Next Gen Sequencing 49
Samples - Cell culture plates/ ss or dsDNA/ PCR products/ Cloned Gene Synthesis 52
vector cells/ Plasmid. Bacterial Identification 53
Primer Designing 83
Re-Sequencing
For detection of mutations in candidate genes or genomic regions
of interest.
Gene Sequencing
Fragment Analysis
Fragment Analysis services for almost all types of fragments like GFA008 Microsattelite analysis using custom labelled primers
AFLP, SNP, SSR, STR, VNTR etc. are provided for the following (for multiplexing;4plex)
applications: GFA009 Microsattelite analysis using custom labelled primers
GENOMICS
(for multiplexing;5 plex)

GFA010 Microsattelite analysis using 96 well reaction format
• Micro-satellite Analysis GFA011 AFLP analysis
• AFLP Analysis GFA012 RFLP analysis
• RFLP Analysis GFA013 SNP analysis
• SNP Analysis GFA014 SSR marker identification
GFA015 Mutation detection in Prokaryotic system
• Mutation Detection GFA016 Mutation detection in Eukaryotic system
• Microbial Identification (16s rRNA Seq.) GFA017 Microbial identifcation (16s rRNA sequencing)
Microsatellite Analysis: For all prices contact : customersupport@ilifediscoveries.com

Five types of dyes which can be analyzed in each sample are:
• 6-FAM • VIC • NED • PET • LIZ Gene Walking
Gene Walking services for template size ranging from 1 kb to 10 kb
Service Information
For high-throughput analysis, multiplex reactions with primers are available for the following.
labeled with different dyes (5 plex maximum) & fragment sizes can
be performed. • PCR amplicons
• Plasmid inserts
• Entire plasmids
Sample submission can be:
• Genomic DNA to produce single strand
• Genomic DNA with primer details
• Culture Plates
• Pre-labeled primers for multiplexing along with the fragments
• BAC or Phage DNA
and thermocycler conditions
• Primer sequences in excel format with fragments (specifying the
Beginning from the reference sequence, stage one of sequencing
labeling type) and thermocylcler conditions
is started, and then onwards, primers are designed to perform
• PCR amplicon pool of the multiplexed reaction for sequencing
overlapping sequencing of the remaining DNA and assemble the
data generation and analysis
sequence data. Additionally, if you have a known sequence for
which you need quality resequencing information, a complete set
Highly accurate data is provided with less than 1bp difference
of overlapping primers will be designed for generating complete
between the sample replicates.
sequence.
SNP Detection
• Upto 4kb of amplicons can be generated.
Single nucleotide polymorphisms (SNPs) are widely used as DNA
• Contigs are assembled using refined bioinformatics tools and
markers in genetic studies. Compared to other SNP genotyping
high quality amplification & sequencing primers are designed
methods, sequencing is in particular, suited to identifying multiple
for Gene walks.
SNPs in a small region, such as the highly polymorphic Major
Histocompatibility Complex region of the genome.
Efficient SNP detection methods on standard DNA sequencers are

available.

GFA001 Microsattelite analysis using unlabelled primers GGW001 Gene walking for upto 1kb
GFA002 Microsattelite analysis using conventionally labelled primers GGW002 Gene walking for upto 2kb
(for multiplexing;2 plex) GGW003 Gene walking for upto 3kb
(for multiplexing;4plex) GGW007 Gene walking for upto 7kb
GFA005 Microsattelite analysis using conventioanlly labelled primers GGW008 Gene walking for upto 8kb
GFA006 Microsattelite analysis using custom labelled primers GGW010 Gene walking for upto 10kb
(for multiplexing;2 plex) GGW011 Gene walking for more than 10kb
GFA007 Microsattelite analysis using custom labelled primers
(for multiplexing;3 plex)
DNA Microarrays
Microarrays are now most extensively used and reliable tools for With wide range of applications iLife helps scientists enhance their
exploring the transcription events of any living cell. Microarrays can drug discovery research.
be a great tool while conducting basic research, understanding
disease mechanisms, exploring stress and environmental tolerance Gene Expression Analysis
mechanisms, identifying new drug targets end discovering • Explore transcriptome through expression profiling
GENOMICS
biomarkers. • Transcription regulators analysis by microRNA profiling
iLife scientists explore a wide variety of genomes across different Location analysis
model systems using the Agilent’s DNA microarray platform. • Explore epigenetic modification by methylation analysis
• Discover protein-DNA interactions through ChIP-on-chip analysis
Focused on Sensitivity
High-purity, high-fidelity 60-mer oligonucleotide probes deliver better
signal-to-noise-ratios and wider dynamic ranges than other
Copy Number Analysis
microarrays. • Explore the chromosomal variations through Comparative
Genomic Hybridization (CGH)
• Discover DNA Copy Number Variants (CNVs) across the
Engineered for Flexibility chromosomes
The SurePrint inkjet technology, enables total control over array
content with no compromise data quality.
Service Information
Optimized for Scalability
The multiplexed arrays have up to eight arrays on a single slide,
making expression studies of any scale feasible and flexible.
DNA Microarrays
Transcriptome Analysis
Gene Expression Analysis
Global gene expression profiling has long been a staple of genomics
GENOMICS
researchers as they seek to elucidate cellular function. Unlike

traditional, hypothesis-driven experimentation, global gene
expression studies are often used to identify candidate genes for
follow-on study.
Microarray technology helps researchers explore the transcriptome
and identify & understand low-abundance transcripts and their
influence on biological systems.
We provide gene expression analysis which helps researchers
working on understating the processes of:
• Differential response to toxic agents
• Regulatory points of complex signaling pathways
• Cancer studies
• Difference between treated and untreated samples
• Infectious disease studies
Service Information
iLife offers end-to-end solutions from sample

preparation to data analysis and deliver
publication ready data.
Genome-wide analysis for human, mouse, rat,
and more
More than 41,000 genes and transcripts are synthesized as 60-
mer oligonucleotides on the whole-genome slides.
Gene expression profiling can be performed either in one color or
two color experiments.
Gene expression microarrays are available in 4×44K format allowing
advantages of hybridizing multiple samples on single slide, improved
dynamic range and contains Spike-in controls and quality control
reporting for high confidence in hybridization results.
One color gene expression

For one-color microarray analysis, the individual sample is labeled
and then hybridzed on one microarray. This simple and flexible Ordering Information
experimental setup compares the measured gene expression of a
microarray directly across other microarrays. Data consistency
improves by performing sufficient technical and biological replicate GGE001 Whole Human Genome Microarray One Color
assays. GGE002 Whole Human Genome Microarray Two Color
GGE003 Whole Mouse Genome Microarray One Color
GGE004 Whole Mouse Genome Microarray Two Color
Two color Gene expression profiling GGE005 Whole Rat Genome Microarray One Color
In the two-color procedure, two samples are individually labeled GGE006 Whole Rat Genome Microarray Two Color
with different fluorochromes such as Cyanine-3 (Cy3) and Cyanine- GGE007 Custom 4 x 44K Microarray GE One Color
GGE008 Custom 4 x 44K Microarray GE Two Color
5 (Cy5). They are hybridized on the same microarray, allowing a GGE009 Custom 2 x 105KMicroarray GE One Color
direct comparison between control versus experimental samples GGE010 Custom 2 x 105K Microarray GE Two Color
there by minimizing variability between slides. GGE011 Custom 1 x 244K Microarray GE One Color
GGE013 Custom 8 x 15K Microarray GE One Color
Data Analysis GGE014 Custom 8 x 15K Microarray GE Two Color
Customized uncompromised high quality publication ready data is GGE015 Gallus (chicken) Genome Microarray One Color
provided in different analysis. GGE016 Gallus (chicken) Genome Microarray Two Color
GGE017 Arabidopsis Genome Microarray One Color
GGE018 Arabidopsis Genome Microarray Two Color
GGE019 Bovine Genome Microarray One Color
DNA Microarrays
Ordering Information microRNA Profiling
Service No. Description Discover Novel Pathways of Gene Regulation
GGE020 Bovine Genome Microarray Two Color
GGE021 Canine Genome Microarray One Color The discovery of microRNAs (miRNAs) has revolutionized our
GENOMICS
GGE022 Canine Genome Microarray Two Color understanding of the regulatory arsenal of the cell. microRNAs are
GGE023 C. elegans Genome Microarray One Color key regulators of gene expression and have been found to be
GGE024 C. elegans Genome Microarray Two Color
modulated in different developmental stages, tissues and disease
GGE025 Corn Genome Microarray One Color
GGE026 Corn Genome Microarray Two Color states.
GGE027 Drosophila Genome Microarray One Color
GGE028 Drosophila Genome Microarray Two Color iLife offers hybridization based miRNA microarray services to profile
GGE029 E.Coli Genome Microarray One Color
entire human, mouse and custom organism miRNAs.
GGE030 E.Coli Genome Microarray Two Color
GGE031 Horse Genome Microarray One Color Hybridization-based microarrays provide maximum flexibility in
GGE032 Horse Genome Microarray Two Color terms of species of interest and target coverage.
GGE033 Magnaportha Genome Microarray One Color
GGE034 Magnaportha Genome Microarray Two Color
Entire workflow starting from isolating very difficult microRNA from
GGE035 Mosquito Genome Microarray One Color
GGE036 Mosquito Genome Microarray Two Color various sample sources till the data analysis is performed in well
GGE037 Pig Genome Microarray One Color optimized and clean room facility ensuring high quality and
GGE038 Pig Genome Microarray Two Color publishable data. Reliable biological interpretation data will be
Service Information
GGE039 Rabbit Genome Microarray One Color
provided from the data analysis report.
GGE040 Rabbit Genome Microarray Two Color
GGE041 Rat Genome Microarray One Color
GGE042 Rat Genome Microarray Two Color The flexible format (8x15K) and service can handle projects focusing
GGE043 Rhesus Monkey Genome Microarray One Color on a small set of candidate microRNAs as well as screening studies
GGE044 Rhesus Monkey Genome Microarray Two Color
designed to profile majority of currently known microRNAs.
GGE045 Rice Genome Microarray One Color
GGE046 Rice Genome Microarray Two Color
GGE047 Salmon Genome Microarray One Color
GGE048 Salmon Genome Microarray Two Color
GGE049 Sheep Genome Microarray One Color
GGE050 Sheep Genome Microarray Two Color
GGE051 Tobacco Genome Microarray One Color
GGE052 Tobacco Genome Microarray Two Color
GGE053 Xenopus Genome Microarray One Color
GGE054 Xenopus Genome Microarray Two Color
GGE055 Yeast Genome Microarray One Color
GGE056 Yeast Genome Microarray Two Color
GGE057 Yeast Genome Microarray One Color
GGE058 Yeast Genome Microarray Two Color
GGE059 Zebrafish Genome Microarray One Color
GGE060 Zebrafish Genome Microarray Two Color
GGE061 Human miRNA Microarray
GGE062 Mouse miRNA Microarray
GGE063 Rat miRNA Microarray
GGE064 Custom miRNA Microarray
DNA Microarrays
Location Analysis
Discover DNA Methylation Patterns
DNA methylation events are heritable modifications that regulate
GENOMICS
gene expression without altering the DNA sequence itself. They

serve as regulatory mechanisms for a wide range of biological
processes and genetic diseases, and irregular methylation patterns
have been associated with certain cancers. As a key epigenetic
mechanism, DNA methylation studies are providing researchers
with a critical avenue for exploring key biological processes.
iLife assists scientists discover critical DNA methylation events using

Agilent’s CpG and promoter microarrays. We also customize arrays
that researchers choose their specific regions or proprietary
sequences for additional evaluation. This helps study of regulatory
networks with confidence.
We provide high quality publication ready data according to the

Service Information
project and offer post service support according to the requirement

of the researcher. Refer page number for detailed description of
the data analysis services.
DNA methylation arrays are available in flexible formats (1x244k to

4x44k) with probes covering the entire genome The Human CpG
Island Microarray contains probes designed with a complete
coverage of the CpG islands & promoters regions about 95kb Ordering Information
upstream and downstream.
GLA001 Human Promoter Set, 2-design(2 slides/array)
GLA002 Human CpG Island Set
GLA003 Human ENCODE Microarray
GLA004 Mouse CpG Island Set
GLA005 Mouse Promoter Set, (2 slides/array)
GLA006 Arabidopsis Microarray
GLA007 C. elegans Microarray
GLA008 Drosophila Microarray
GLA009 Zebrafish Promoter
GLA010 Yeast Whole Genome Set
GLA011 S. pombe Microarray
GLA013 Custom 1x244K DNA Methylation Single Owner
GLA016 Custom 8x15K DNA Mthylation Single Owner
GLA017 SurePrint 1X1M G3 CH3 Custom
GLA018 SurePrint 2X400K G3 CH3 Custom
DNA Microarrays
Chip-on-chip Analysis
Transcription is a complex process that requires multiple interactions
and orchestrated binding of numerous components. Thus, traditional
gene expression analysis provide only partial glimpses into gene
GENOMICS
regulation, whereas ChIP-on-chip reveals a high-resolution map of
transcriptional activities, including activation and repression.
By partnering, we enable you to obtain insightful and robust profiles
of DNA-protein binding and promoter occupancy across entire
genomes.
We offer services on Agilent’s catalog Human ENCODE ChIP-on-

chip Microarray and Human Promoter microarray which specifically
identifies human DNA binding proteins within the ENCODE &
Promoter regions through traditional chromatin immunoprecipitation
(ChIP) pull-down coupled with powerful DNA microarray location
analysis.
Service Information
Genome-Wide Location Analysis
We help scientists explore the genome and perform location analysis
through ChIP-on-chip microarray featuring a total of ~244,000 60-
mer probes. Probes are spaced every ~100-300 bp across regions
of interest in both coding and noncoding DNA sequences.
Focused microarray designs include human and mouse promoter
sets. Whole genome designs include human, mouse, Drosophila,
C. elegans, and Arabidopsis microarray sets.
Applications of Agilent ChIP-on-chip

• Uncover and validate gene regulation and regulatory networks
by comprehensive determination of promoter occupancy.
• Identify and characterize molecular events associated with
processes for transcription, DNA replication and repair, as well
as with chromatin modifications and DNA methylation.
• Elucidate modes of action and potential therapeutic activities of
compounds and target genes by mapping gene regulatory
networks relevant to disease and pathophysiological states.
• Validate and augment existing gene expression data with
authentic binding events.
• Identify, assess, and monitor biomarkers responsive to protein- Ordering Information
DNA binding events to serve as bioassays or toxicant signatures
for toxicogenomics.
GLA021 Human Promoter Set, 2-design(2 slides/array)
• Uncover and profile off-target events as well as validate primary GLA022 Human CpG Island Set
and secondary effects in screening of candidate compounds, GLA023 Human ENCODE Microarray
siRNAs, therapeutics, etc. GLA024 Mouse CpG Island Set
GLA025 Mouse Promoter Set, (2 slides/array)
GLA026 Arabidopsis Microarray
Data Analysis GLA027 C. elegans Microarray
GLA028 Drosophila Microarray
High quality publication ready data is provided under the GLA029 Zebrafish Promoter
bioinformatics support. GLA030 Yeast Whole Genome Set
GLA031 S. pombe Microarray
GLA033 Custom 1x244K ChIP-on-Chip Single Owner
GLA037 SurePrint 1X1M G3 ChIP Custom
GLA038 SurePrint 2X400K G3 ChIP Custom
DNA Microarrays
Copy Number Analysis
Comparative Genomic Hybridization (CGH)
Genomic instability is a classic hallmark of cancer genetic disorders.
GENOMICS
Traditional methods of comparative genomic hybridization (CGH)

have enabled rough analysis of chromosomal variations, but are
imprecise and low-throughput. To map chromosomal changes,
aberrations with superior precision, oligo-based array-Comparative
genomic hybridization (aCGH) is a better approach with respect to
genetic diseases.
We offer project based complete solution to the scientist interested

in exploring chromosomal changes in:
developmental abnormalities, disease susceptibility, and
differential drug responses associated with :
• Genetic disorders
• Cancer
• Karyotyping
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• Evolutionary Biology
Services are provided on catalog CGH arrays covering entire

Human, Mouse and Rat Genomes in higher precision 1x244k and
2x105k formats that enable better resolution in mapping
chromosomal breakpoints and identifying novel microvariations. Ordering Information
We can also provide deletion analysis on a custom 4x44k array Service No. Description
containing 43,000 probes with an average probe spatial resolution GCN001 Human Genome 1 x 244K CGH Microarray Kit
of 35 kb and an emphasis on the most commonly studied genomic GCN002 Human Genome 2 x 105K CGH Microarray Kit
coding regions and cancer-related genes. GCN003 Human Catalog 4 x 44K CGH Microarray Kit
GCN004 Mouse Genome 1 x 244K CGH Microarray Kit
GCN005 Mouse Genome 2 x 105K CGH Microarray Kit
Apart from the catalog arrays project specific arrays can be GCN006 Rat Genome 1 x 244K CGH Microarray Kit
customized with enhanced coverage of known genes, promoters, GCN007 Rat Genome 2 x 105K CGH Microarray Kit
miRNAs, disease pseudoautosomal and telomeric regions. GCN008 Chicken Genome 2 x 105K CGH Microarray Kit
GCN009 Custom 1 x 244K HD-CGH Microarray,
GCN011 Custom 4 x 44K HD-CGH Microarray
GCN012 Custom 8x15K HD-CGH Microarray
GCN013 SurePrint G3 custom 1x1M CGH array
GCN014 SurePrint G3 custom 2X400K CGH array 2x400K
GCN015 SurePrint G3 custom 4x180K CGH array
GCN017 Cyto Custom 1 x 244K HD-CGH Microarray
GCN020 Cyto Custom 8x15K HD-CGH Microarray
DNA Microarrays
Copy Number Variation (CNV)
Genetic variation is what makes each human being unique, and
determines our susceptibility to diseases. In recent years,
researchers have utilized SNPs for the identification of genetic
GENOMICS
markers for complex and common diseases; however, this approach
has only revealed part of the picture. Copy number variants (CNVs)
are reshaping our understanding of the genetics of a growing list of
diseases, and providing a deep insight into the role that these
variants play in genetic diseases. The use of high-resolution aCGH
microarrays to study CNVs enables researchers to get an extremely
fine view of variant structure and impact.
We use Agilent SurePrint G3 and High definition CNV microarrays

to offer scientists comprehensive genomic coverage that enables
the simultaneous identification and quantification of CNVs at
multiple loci with maximum specificity and sensitivity.
Service Information
CNV microarrays are available in 1x244k and 1x1M formats
depending on the overall spacing of the tiled probes representing
human coding and noncoding sequences.
We offer complete project based services starting from the DNA

isolation till data analysis and provide publication ready data keeping
in view customers requirements and by providing excellent post
service support.
GCN023 Human Genome 1 x 244K CNV Microarray Kit
GCN024 Human Genome 2 x 105K CNV Microarray Kit
GCN025 Human Catalog 4 x 44K CNV Microarray Kit
GCN026 Mouse Genome 1 x 244K CNV Microarray Kit
GCN027 Mouse Genome 2 x 105K CNV Microarray Kit
GCN028 Rat Genome 1 x 244K CNV Microarray Kit
GCN029 Rat Genome 2 x 105K CNV Microarray Kit
GCN030 Chicken Genome 2 x 105K CNV Microarray Kit
GCN031 Custom 1 x 244K HD-CNV Microarray,
GCN033 Custom 4 x 44K HD-CNV Microarray
GCN034 Custom 8x15K HD-CNV Microarray
GCN035 SurePrint G3 custom 1x1M CNV array
GCN036 SurePrint G3 custom 2X400K CNV array 2x400K
GCN037 SurePrint G3 custom 4x180K CNV array
GCN039 Cyto Custom 1 x 244K HD-CNV Microarray
GCN042 Cyto Custom 8x15K HD-CNV Microarray
GCN043 SurePrint G3 2x400K Human CNV array
GCN044 Human catalog 2x105k CNV Microarray kit
DNA Microarrays
Custom Microarray Development Ordering Information
Whether studying the genome of a unique organism or digging GGE007 Custom 4 x 44K Microarray GE One Color
deep into a geneset of interest, customization is possible for any GGE008 Custom 4 x 44K Microarray GE Two Color
GENOMICS
genome, any application, anytime and anywhere. GGE009 Custom 2 x 105KMicroarray GE One Color
GGE011 Custom 1 x 244K Microarray GE One Color
iLife helps you design microarrays and exclusively customize arrays
for specific applications including : GGE013 Custom 8 x 15K Microarray GE Two Color
• gene expression GGE014 Custom 8 x 15K Microarray GE Two Color
• microRNA profiling GGE064 Custom miRNA Microarray
• DNA methylation
• Comparative genomic hybridization (aCGH) GLA015 Custom 2x105K DNA Methylation Single Owner
• ChIP-on-chip GLA016 Custom 8x15K DNA Methylation Single Owner
• model organisms GLA017 SurePrint 1X1M G3 CH3 Custom
We help you select probe content from wide range of high definition GLA020 SurePrint 8X60K G3 CH3 Custom
databases present on Agilent eArray portal. Oligonucleotide probes GLA033 Custom 1x244K ChIP-on-Chip Single Owner
are also custom designed from the genes and transcript annotations GLA034 Custom 4x44K ChIP-on-Chip Single Owner
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present in public databases such as RefSeq, Unigene, TIGR,
GenBank etc. or from the sequences generated by the customer GLA037 SurePrint 1X1M G3 ChIP Custom
for specific organism of interest. This allows characterizing the GLA038 SurePrint 2X400K G3 ChIP Custom
microarrays with excellent genome coverage linking all essential GLA039 SurePrint 4X180K G3 ChIP Custom
genomic and proteomic databases.
We offer the flexibility of choosing any number of genes and GCN011 Custom 4 x 44K HD-CGH Microarray
multiplexing the arrays on a single slide (Formats. 4x44k, 8x15k, GCN012 Custom 8x15K HD-CGH Microarray
GCN013 SurePrint G3 custom 1x1M CGH array
2x105k, 1x1M etc.)
GCN014 SurePrint G3 custom 2X400K CGH array 2x400K
GCN020 Cyto Custom 8x15K HD-CGH Microarray
GCN032 Custom 2 x 105K HD-NV Microarray,
GCN033 Custom 4 x 44K HD-CNV Microarray
GCN034 Custom 8x15K HD-CNV Microarray
GCN035 SurePrint G3 custom 1x1M CNV array
GCN036 SurePrint G3 custom 2X400K CNV array 2x400K
GCN042 Cyto Custom 8x15K HD-CNV Microarray

Service Page No.
DNA Extraction & Purification 20
RNA Extraction & Purification 20
DNA Analysis 21
RNA Analysis 22
Real Time PCR Assays 43
Microarray Data Analysis 76
Custom Chip Designing & Analysis for Microarray 80
Real Time PCR Assays
Real Time QPCR Assays
Real Time quantitative PCR is used as a quantitative tool for gene
expression nowadays. QPCR is a powerful, highly sensitive
technique that enables both detection & quantitation (as absolute
GENOMICS
number of copies or relative amount when normalized to DNA input
or additional normalizing genes) of one or more specific sequences
in a DNA sample. iLife Discoveries supports both Taqman & SYBR
Green chemistries for detection of products in real time PCR.
We use a wide range of fluorescent dye wavelengths for detection

from 350 nm to 700 nm consisting of four dyes FAM, Cy5, HEX
and ROX.
We offer complete services from sample preparation till data analysis

which also include primer and probe designing, synthesis and
validation services.
Service Information
Real Time QPCR delivers powerful capabilities for generating and
analyzing data for variety of applications:
• Validation of Microarray data

• DNA/RNA Copy number Assessment
• Primer Efficiency determination
• Allele Discrimination
• Load Detection (viral, bacterial or fungal)
• SNP Analysis
Sample Submission Guidelines
• Splice Variant Detection
• Mutational Analysis
• Residual Disease Detection Sample Type Minimum Sample
• Detection of Chromosomal translocations RNA 500ng-1000ng
• Screening of GMOs
Tissue 15-30mg
• miRNA Detection
Bacterial Cells 1.5-2ml( 115 cells/ml)
Mammalian Cells 1.5-2ml( 110 cells/ml)
Whole Blood 1.5 ml
Yeast cells 1.5-2ml( 112 cells/ml)
GRT001 SYBR-Green based QPCR
GRT002 Taqman-based QPCR
GPR001 Primer Designing, Synthesis and Validation for
SYBR-Green Detection
GPR002 Probe & Primer Designing, Synthesis and
Validation for Taqman Detection
Absolute Quantification by qPCR Relative/Comparative Quantitation
Real Time qPCR can be used for Absolute Quantification of unknown Comparative quantification of genes is an application of RT-PCR
samples. It is a method used to quantify unknown samples compared which is used for calculation of relative abundance of a gene in
to samples of known concentration. The Ct values of the unknowns treated sample with respect to the control sample in terms of fold
GENOMICS
can now be compared to the standard curve to determine the initial change or copy number.
quantity of template in these samples. We offer complete solution from sample preparation to data analysis
which includes Relative Quantity Charts where relative
We offer complete solution from sample preparation to data concentration of gene of interest is measured in unknown sample
generation which includes standard curve, Initial template quantity, compared to the control.
Threshold cycle number, amplification plots, plate sample values,
text report. Data is generated in the form of amplification plots, raw fluorescence
values, plate sample values and text report.
Our services can be utilized for applications of :
• Viral Load Detection We can perform relative quantitation services for applications of :
• Genomic and Mitochondrial DNA Detection (Forensics) • Gene Expression
• DNA/RNA Copy number ssessment • Microarray data validation
• Primer Efficiency determination • Pathogen Detection
• GMO Detection
Service Information
• Environmental Testing
Sample submission Requirement : • Infectious Diseases
• Standards for plotting of standard curve
• Unknown samples for quantification Sample Submission Guidelines
• Calibrator (a positive control of known concentrations that all
samples will be compared for the up-regulation and down
regulation of gene levels)
• Infected samples containing unknown concentration of gene of
interest
• Housekeeping gene required for constant level expression in
calibrator and infected samples.
GAQ001 Absolute Quantitation with Standard Curve
GAQ002 Absolute Quantitation without Standard
Curve
GRQ001 Relative SYBR Green Quantitation with House Keeping genes
GRQ002 Relative SYBR Green Quantitation without House Keeping genes
GRQ003 Relative Taqman Quantitation with House Keeping genes
GRQ004 Relative Taqman Quantitation without House Keeping genes
Multiplex Real Time Assay Applications
Multiplex QPCR allows one or more targets of interest to be Validation of Microarray data
amplified with a normalizing gene in the same reaction vessel by Quantitative real-time PCR (qPCR) is a commonly used validation
using probes with spectrally different fluorophores for the detection tool for confirming gene expression results obtained from microarray
GENOMICS
of each amplicon. Generally experimental projects require many analysis.
runs on the same set of genes. Microarray analysis performs hundreds or thousands of differentially
expressed genes simultaneously while qPCR provides precise
iLife provides cost effective and higher level of statistical correlation quantification of a wide dynamic range of expression levels due to
to design a multiplex reaction to use for the duration of the study. its increased sensitivity and reproducibility. We can rapidly validate
The multiplex reaction is important when the template material is and correlate microarray data through the qPCR approach.
limited as this approach allows maximum amount of data to be We identify real gene specific variation, as it is important to
generated from each assay. The primers and probes for multiplex normalize gene expression level by carefully selected stable internal
reactions will be designed the same way as for singleplex reactions control genes.
with a few extra considerations.
Gene Expression Workflow
We offer complete solutions from Sample preparation to data
analysis which includes raw fluorescence data, amplification Sample preparation and isolation of RNA
Service Information
plots, threshold, dissociation curve (only in case of SYBR
Green) & standard curve Amplification and labeling
Applications Microarray processing and analysis

• Microbial Diagnostics
• Infectious diseases QRT-PCR validation
• Bacterial,Viral identification
Molecular Diagnostics
The real time PCR provides high-throughput method for faster and
Ordering Information more sensitive and more specific methods to diagnose viral
pathogens.
GMR001 Multiplex QPCR Assay
Infectious diseases
The prevalence of disease in patients is exacerbated by inadequate
For all prices contact : customersupport@ilifediscoveries.com methods for pathogen detection. PCR-based amplification
approaches have been developed to address this problem because
conventional methods for pathogen identification lack sensitivity,
specificity and speed, and some infectious organisms are difficult
to culture. PCR amplification of ribosomal genes and their internal
transcribed spacer regions coupled with sequence-specific
detection probes are the most reliable approaches for disease
identification.
Metabolic Disorders Serial Dilution Standard Curve
The introduction of real-time PCR provides the opportunity for the
rapid detection of pathogens in food and clinical settings. Apart
from saving time, real-time PCR is highly specific and sensitive
and offers the potential for quantification .The risk of cross-
GENOMICS
contamination is significantly reduced, and high-throughput

performance and automation are possible since no post-PCR
manipulations are required.
Child and Women health

The Indian population comprises of a large mass of children and
women who are facing health problems. The introduction of
Molecular Beacon probe and Taqman probe allows more rapid and
specific identification of pathogens.
Efficiency = [10(-1/slope) ] - 1
Primer Efficiency Determination DNA/RNA copy number assessment

Primer Efficiency can be easily determined with Standard curve Bacterial, Viral and Fungal Load Detection
plotting that would test the run and determine the overall assay Real time PCR is an appropriate technique for the identification
Service Information
performance. and quantification of Bacteria viruses and fungus. Emerging Micro-

A dilution series is prepared in triplicates along with the negative organisms need to be identified and characterized in terms of the
control and a minimum concentration of primer is required for prevalence in the population and their relevance.
plotting of the standard curve.
The software also provides dissociation curve that determines the Data Generated:
specificity of primers, the presence of primer - dimer in the wells • Initial copies calculated through Standard Curve plotting
and non-specific amplification present if any. • Plate sample values(Florescence or threshold cycle number)
SYBR Green Melting Curve Analysis

·
Primer Optimization Matrix
Optimize concentration of forward and reverse primers and total

primer concentration.
Environmental Testing using Labeled Probe when coupled with the advanced technology of RT thermal cyclers
with reduced ramp times, greatly reduces cycle times so that
Micro-organism population dynamics assessed by real time amplified targets can be recognized within 30 min of amplification
quantitative PCR in the environment. A real time quantitative PCR in some cases
(QPCR) is used to detect and quantify the 16S rRNA gene
GENOMICS
concentration Screening of GMOs
A Real time PCR screening is done to detect specific strains of Q-PCR is used to measure the quantity of a PCR product. It is the
micro-organisms in environmental samples. Fluorescently labeled method of choice to quantitatively measure amounts of transgene
probe are designed against the targets i.e. a short segment of the DNA in a food or feed sample.If the targeted genetic sequence is
micro-organism DNA which is highly specific to the probe unique to a certain GMO, a positive PCR test proves that the GMO
is present in the sample.
SNP Analysis Using Real Time PCR

Single nucleotide Polymorphisms (SNPs) are considered to be next
generation markers for the identification of loci associated with
complex diseases and for pharmacogenetic applications. SNPs
allow the unification of the candidate gene approach and
association-based fine mapping to identify gene(s) of interest. They
also aid in the association of linkage analysis to the phenotypic
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and genotypic data.
Real Time PCR allows a fast, sensitive and elegant quantification
of genotypes based on SNP. We provide a customized solution for
accurate quantification of SNP allele-specific respective informative
locus analysis using real time PCR. The SNP analysis can be useful
in the following areas:
• Allelic Association
Microbial Diagnostics
• Linkage Disequilibrium
Initially, implementation of the polymerase chain reaction (PCR)
• Positional Cloning
resulted in sensitive detection of microbes that were hard or
• Disease Mapping
impossible to diagnose by conventional diagnostic procedures such
• Pharmacogenetics
as culture and serology. A further improved version is now rapidly
• Mutational Analysis
replacing end-point detection PCR in many diagnostic laboratories.
In real-time PCR, amplified products are detected by fluorescence
at the moment that they are generated and directly related to the References
input target quantity, so that quantitation is possible. 1. Collins A.L., Lonjou C., Morton N.E. (1999) Proc. Nat. Acad. Sci.
96:15173–15177.
The probe includes an internal amplification control and primers 2. Lander E.S. (1996) Science 274:536–539
and a probe designed to target a highly specific part of the genes. 3. Lander E.S., Schork N.J. (1994) Science 265:2037–2048.
Splice Variant Detection Using Real time PCR

Alternative splicing is a widespread process used in higher
eukaryotes to regulate gene expression and functional diversification
of proteins. Investigating the expression levels of splice variants is
an important step for knowing the significance of each variant.
Real Time PCR is a very well suited technique to quantitate
alternatively spliced transcripts. It can use for studying varied
applications:
References: • Alternatively spliced messenger RNAs
Applied and Environmental Microbiology, March 2007, p. 1892- • Exon-specific Quantitation
1898, Vol. 73, No. 6 • Analysis of cytokine mRNA
Food and Agricultural References

RT-PCR assays have been developed for a variety of target 1. Ina I. Vandenbroucke, Jo Vandesompele, Anne De Paepe and
sequences of food borne microbial pathogens, plant pathogens, Ludwine Messiaen* Department of Medical Genetics, University
and genetically modified foods. A major and highly sensitive aspect Hospital Ghent-OK5, De Pintelaan 185, 9000 Ghent, Belgium
of RT-PCR is the use of fluorescent reporter dyes that undergo 2. Gibson, U.E., Heid, C.A. and Williams,P.M. (1996)
enhanced fluorescence when bound to DNA. A major advantage of 3. Gilliland, G., Perrin,S., Blanchard,K. and Bunn,H.F. (1990)
RT-PCR involves the amplification of short DNA target sequences 4. Kafert, S., Krauter,J., Ganser,A. and Eder,M. (1999)
of 60-70-bp, which allows greatly reduced extension times, which 5. Favy,D.A., Lafarge,S., Rio,P., Vissac,C., Bignon,Y.J. and Bernard-
Gallon,D. (2000)
Cancer Diagnostics using Real Time PCR Ordering Information
Cancer-specific Molecular Markers can be used to provide accurate
prognosis and to predict response, resistance, or toxicity to therapy.
GQA001 Validation of Microarray data
The diversity of genomic alterations involved in malignancy
GQA002 Molecular Diagnostics for Infectious diseases
necessitates a variety of analysis for complete tumor profiling in
GENOMICS
GQA003 Molecular Diagnostics for Metabolic disorder

clinical laboratory. Real-time PCR methods are an attractive option GQA004 Molecular Diagnostics for Child and Women Health abberations
for the analysis of cancer markers. GQA005 Primer Efficiency Determination by SYBR
Green Melting curve analysis
It is a rapid, versatile, and cost-effective technique and can be
GQA006 Primer Efficiency Determination by Primer optimization Matrix
performed on small tissue samples. Real-time PCR plays an GQA007 Primer Efficiency Determination by Serial Dilution Standard Curve
increasingly important role in clinical testing because it can provide GQA008 DNA/RNA copy number assessment
information about gene expression, gene amplification or loss, and GQA009 Environmental testing using labeled probe
GQA010 Microbial Diagnostics
small alterations (e.g., point mutations). Few applications are listed
GQA011 Screening of GMOs
below: GQA012 Allelic Association detection
• Mutation Detection in solid tumors GQA013 Linkage Disequilibrium profiling
• Residual Disease detection GQA014 Positional Cloning determination
GQA015 Disease Mapping
• Molecular diagnostics
GQA016 Pharmacogenetics application
GQA017 Mutation Analysis
References GQA018 Splice Variant Detection Using Real time PCR
GQA019 Cancer Diagnostics using Real Time PCR
Service Information
1. Philip S. Bernarda1 and Carl T. Wittwer1 Clinical Chemistry 48:

GQA020 Forensic Stain Identification
1178-1185, 2002. GQA021 Forensic DNA/ RNA Quantification
2. Eleftherios P. Diamandis1 and David E. Bruns2 Clinical Chemistry GQA022 Human Identity Testing
48: 1145-1146, 2002. GQA023 STR Profiling
GQA024 Customized Realtime PCR Assays
Real Time PCR in Forensic Science For all prices contact : customersupport@ilifediscoveries.com
Quantification of DNA in a forensic sample is of major importance
for proper DNA amplification and STR profiling. The implementation
of molecular analysis in the forensic laboratory has been a
tremendous benefit to the criminal justice community.
Investigation of forensic samples using real time PCR method helps
in detection with increase specificity and sensitivity. This technology
can be employed in various testing such as: Service Page No.
• Forensic Stain Identification DNA Microarrays 35
• Forensic DNA/ RNA Quantification RT-PCR Data Analysis 81
• Human Identity Testing High-end Technology Training 88
• STR Profiling
References
1. Trisha L. Noreault-Conti, Ph.D., and Eric Buel, Ph.D. Vermont
Forensic Laboratory, Department of Public Safety, March 2007.
2. Nicklas JA, Buel E. Anal Bioanal Chem. 2003 Aug; 376(8):1160-
7. Epub 2003 May 9.
Next Gen Sequencing
Next Generation Sequencing is changing the genetics landscape RNA-sequencing can be combined with Paired-Ends for sequencing
and opening new avenues to expand research and development. ranscriptomes from non-model organisms for which no reference
We offer customized solutions for a full range of next Gen is available. Even if you do not need the expression data, RNA-
sequencing applications such as : sequencing can also be a more cost effective strategy for
• Whole genome sequencing sequencing the gene space of an organism not previously
GENOMICS
• mRNA/Transcriptome sequencing sequenced.
• ChIP sequencing
• miRNA and Small RNA discovery RNA-sequencing profiles are also more versatile than microarray
• DeNovo Sequencing expression profiles as they are amazingly reproducible and can
• SNP Discovery represent an absolute rather than relative quantitation. In this way,
• Sequence capture data sets produced over many runs can be easily compared. Further,
• Targeted Resequencing it is possible to make gene-to-gene comparisons instead of only
• Multiplexing sequencing treatment-to-treatment comparisons of the same gene given by a
microarray.
Today’s scientific evnvironment, both commercial and academic,
necessitates moving beyond studying markers and genes to full iLife can provide Next Gen Sequencing service with
genome, transcriptome and epigenome characterization. Next Gen just an input of 10 micrograms of total RNA
even enables previously impractical experiments such as
environmental, population and metagenomics.
Service Information
While this technology offers new promise to life science and medical
researchers, it also creates complex challenges in data analysis.
We offer excellent bioinformatics support in analyzing terabytes of
data and providing satisfactory and publishable results.
Next Gen sequencing is a project based customized service. Please

contact us to have a detailed discussion on the technical details
and the workflows before deciding on the type of sequence to be
performed.
Whole Genome Sequencing

Whole genome characterization by shotgun sequencing can be
performed both by single pass fragment analysis and also by paired
end analysis f fragments from total DNA, PCR products, etc.
Fragment reads are used to detect SNPs and small indels in
previously sequenced genomes. Paired End reads are used for
confident genome assembling and to detect larger structural
variants, including chromosomal rearrangements and large indels.
Fragment libraries are typically constructed from 5 micrograms of
total DNA.
Fragment and Paired End analysis will be performed

from 5 micrograms of total DNA
Whole Transcriptome Analysis

This service includes Quantitative transcriptome profiling by
sequencing cDNAs constructed from messenger RNA isolated from
total RNA.
By sampling randomly from total RNA, RNA-Sequencing yields

counts that are as quantitative as qRT-PCR but across a space
more broad than a microarray - the entire transcriptome!
Next Gen Sequencing
ChIP sequencing Micro RNA Analysis
Unlike the ChIP-on-chip microarrays here is a technology that MicroRNA sequencing is performed for the Discovery & quantitation
involves Discovery & quantitation of protein-DNA interactions by of novel microRNAs and isoforms by sequencing cDNAs of
sequencing DNA from immunoprecipitations. microRNAs isolated from total RNA.
GENOMICS
If the DNA is recovered by immunoprecipitation, we can sequence Similar to RNA-sequencing, microRNA sequencing samples
it! We offer sequencing IP products from transcription factors, quantitatively from the entire small RNA population of the cell,
histone modifications, and RNA binding-proteins. including most RNAs smaller than tRNAs. From this data we can
simultaneously discover the expressed microRNA population of
Using specific antibodies, ChIP-sequence protocols call for the the sample as well as build an expression profile that can be
cross-linking of DNA/RNA with neighboring proteins, sonicating the compared to other samples.
fragments until they are very small (~200bp), and then purifying
the DNA/RNA. We will need just 20 micrograms of total RNA and the following
steps of microRNAs extraction, cDNA preparation, library
construction, sequencing and analysis will be performed by us.
In this way we can define the actual binding locations of proteins
genome-wide. Alternatively, we can provide whole-epigenome
profiling by sequencing the ChIP products of one or many histone
Service Information
modifications. Ordering Information

We offer the ChIPsequence service with any total amount of DNA
GNS001 Whole Genome Sequencing
but including at least 20 nano-grams of DNA in the 150-250 bp
GNS002 Whole Transcriptome Sequencing
size range. GNS003 ChIP Sequencing
GNS004 miRNA Sequencing
GNS005 Customized Next Gen Sequencing Services
Next Gen Sequencing
SureSelect Target Enrichment System
(Illumina Single-End Sequencing Platform Library Prep)
SureSelect Target Enrichment System significantly improves the
GENOMICS
cost- and process-efficiency of next-generation sequencing. Based
on an extremely efficient hybrid selection technique, the SureSelect GST001 Customized SureSelect Target Enrichment System
Target Enrichment System can be automated and easily scaled to
meet the needs of larger sequencing projects. This is unlike any For all prices contact : customersupport@ilifediscoveries.com
other commercially available target enrichment method. With
sample input requirements at or below 3 µg of gDNA, researchers
can perform highly targeted next-generation sequencing on the most
precious of samples.
SureSelect Target Enrichment System Workflow
SureSelect platform currently supports target enrichment on both Other Related Services
the Illumina Genome Analyzer and the Applied Biosystems™ SOLiD
Service Page No.
System. Protocols and kits have been optimized for in-solution works
that are custom designed, along with a catalog product targeting DNA Extraction & Purification 20
RNA Extraction & Purification 20
human exome.
Service Information
DNA Analysis 21
RNA Analysis 22
The platform currently consists of the following offerings:
1. SureSelect Target Enrichment System: A custom, in-
solution method useful for large, higher throughput studies.
2. SureSelect All Exon Kits: A single tube assay that targets all
human exons with significant depth of coverage. 1.22% of human
genomic regions are covered, corresponding to the CCDS exons.
3. SureSelect All Exon PLUS Kits: Now you can add your own
custom content! The design covers 38Mb (CCDS + >1,000 ncRNA)
and you can add up to 6Mb of your own content
Gene Synthesis
Why use gene synthesis instead of PCR cloning? Ordering Information
PCR cloning, the most common method of obtaining genes, may
not be able to produce a gene of sufficient quality or even at all. Service No. Description
First, a cDNA library for a specific type of tissue has to be prepared GGS001 Eukarotic gene synthesis (including codon
or purchased, which requires either time or expense. Second, the optimization; standard delivery ~4µg)
GENOMICS
GGS002 Eukarotic gene synthesis (including codon

gene must be abundant in that cDNA library or it will be very difficult
optimization; custom delivery >5µg)
to clone any useful way. Third, when PCR is successful, its products GGS003 Eukarotic gene synthesis (excluding codon
may have mutations or single nucleotide polymorphisms (SNP), optimization; standard delivery ~4µg)
which can cause problems during experimentation or other GGS004 Eukarotic gene synthesis (excluding codon
optimization; standard delivery >5µg)
applications.
GGS005 Prokaryotic gene synthesis (including codon
optimization; standard delivery ~4µg)
Key Features GGS006 Prokaryotic gene synthesis (including codon
• There is no length limit. We routinely synthesizes genes of 10 GGS007 Prokaryotic gene synthesis (excluding codon
kb or longer. optimization; standard delivery ~4µg)
• Standard vector: pUC57; synthetic genes will be cloned into GGS008 Prokaryotic gene synthesis (excluding codon
Sma I or EcoR V sites of the standard vector.
GGS009 Custom Plasmid preparation of
• We can subclone the synthetic gene into the vector of your Research grade (Predominantly supercoiled)
choice; the vector must be provided by the customer and must GGS010 Custom Plasmid preparation of SC grade
be a commercial vector with a full-length sequence. (e” 95% Supercoiled, d” 0.03 EU/ug Endotoxin)
Service Information
GGS011 Custom Plasmid preparation of Advanced SC

• We can synthesize the 5'-end or 3'-end of the gene and anneal
grade (e” 95% Supercoiled, d” 0.005 EU/ug Endotoxin)
it to your partial clone to obtain the full-length gene, provided GGS012 Other customized gene synthesis services
that you have the sequence.

Service Page No.
Steps
• Oligo synthesis Customized PCR Assays 26
Cloning 27
• Obtaining full-length genes
Gene Sequencing 33
• Mutation correction Protein Extraction & Purification 56
• Sequence confirmation Protein Expression & Purification 57
Protein Profiling 60
Immunological Assays (ELISA & ELISPOT) 64
Our codon optimized genes Antibody Production 68
It matters as much as protein expression matters. Different Peptide Synthesis 69
organisms have different codon usage preferences. For example,
the preferred codons used in a human gene may be rare in bacteria. Primer Designing 83
This can cause problems when researchers attempt to express
human genes in E. coli. Using our optimized synthetic genes, many
of our customers have reported dramatic increases in protein
expression.
Bacterial Identification
Bacterial Species identification is challenging because of the huge
diversity of morphologically complicated organisms. 16S rRNA
sequencing is one of the most accurate and validated methods for
taxonomical identification of species.
GENOMICS
We provide services for complete workflow starting from DNA
isolation to 16S rRNA sequencing and in-silico testing services for
taxonomical identification.
We offer the following services:

• Identification of a bacterial strain and taxonomical classification.
• Taxonomical confirmation of the bacteria.
• Comparative analysis of the bacteria with closely related species.
• Phylogenetic arrangement of the bacteria.
• Comparison of the bacterial sequence with the Genbank
database through multiple sequence alignment and identify
known and novel bacteria.
• Mining known bacteria from a mixed population by specific
primers.
Service Information
GPD001 Primer designing, synthesis and optimization
GBI001 Bacterial Identification without cloning
GBI002 Bacterial Identification with cloning
PROTEOMICS & CELL BIOLOGY
Service Information
Service Information
Proteomics &
Cell Biology
Simple Solutions-Complex Proteins
PROTEOMICS & CELL BIOLOGY Protein Analysis
Protein Extraction & Purification His-tag protein purification with Ni++ ZipTips® Enzymatic removal
of His Tags from recombinant proteins Complementing RP-HPLC
protein purification
It is a difficult task to extract Proteins in good amount, from many
tissues. iLife offers to extract & purify highly pure protein in small
Antibody Analysis
and large-scale. We have been routinely extracting considerable
Analysis of antibodies under reducing and non-reducing conditions
amount of protein, for various proteomics applications, from various
Quantitation of the half-antibody content in IgG4 preparations
sources including tissue (fresh and preserved), blood, saliva, other
Comparison of SDS-PAGE, CGE and Agilent 2100 bioanalyzer for
body fluids, cell cultures, plants, and forensics samples.
humanized monoclonal antibody analysis Absolute quantitation of
IgG Quality control of stressed antibodies Separation of bispecific
Protein Analysis antibodies chains
platform for sizing, quantification and quality control of Proteins. Food Analysis
For detailed technical information kindly refer to the page no:14. Bovine milk analysis Protein pattern of different transgenic seedlines
Different kits allow Protein quality control – detection at ng & pg Protein - Others
level: Absolute protein quantitation Glycoprotein sizing Protein quality
control prior to MS-analysis Depletion of high abundant proteins
Protein 250 kit: from blood samples Increased sensitivity by desalting protein
Service Information
It detects proteins down to 1 pg/uL on chip, which is superior to samples

silver stain SDS-PAGE. It provides quantification over a dynamic
range of up to 4 orders of magnitude, is highly reproducible, Note: We offer Customized solutions for Protein applications. Please
which is particularly important for the analysis and quality control contact technical support for further assistance.
of proteins.
Protein 230 and Protein 80 kits: Service No. Description
It can be used to:
PPA001 Analysis of cell lysates - protein induction
• Analyze a wide range of of samples PPA002 Comparison between lysate and flow through
• To express recombinant proteins PPA003 Analysis of protein purification
• Purify proteins PPA004 GFP Streptag fusion protein purification
PPA005 Analysis of column capacity
• Perform stability studies PPA006 Analysis of column fractions to optimize conditions
• Check antibody quality down to 10 ng/µl. PPA007 Analysis His-tag protein purification with Ni++ ZipTips®
PPA008 Analysis of Enzymatic removal of His Tags from recombinant proteins
PPA009 Complementing RP-HPLC protein purification
Ordering Information : PPA010
PPA011
Analysis of antibodies under reducing and non-reducing conditions
Quantitation of the half-antibody content in IgG4 preparations
PPA012 Comparison of SDS-PAGE, CGE and Agilent 2100
Service No. Description bioanalyzer for humanized monoclonal antibody analysis
PPA013 Absolute quantitation of IgG
PPE001 Protein Extraction & Purification from Tissue PPA014 Quality control of stressed antibodies
PPE002 Protein Extraction & Purification from Cell Lysate PPA015 Separation of bispecific antibodies chains
PPE003 Protein Extraction & Purification from Culture Supernatant PPA016 Bovine milk analysis
PPA017 Protein pattern of different transgenic seedlines
PPE004 Protein Extraction & Purification from Blood Plasma PPA018 Absolute protein quantitation
PPE005 Protein Extraction & Purification from Body Fluids PPA019 Glycoprotein sizing
PPA020 Protein quality control prior to MS-analysis
PPE006 Customized Protein Extraction & Purification Services PPA021 Depletion of high abundant proteins from blood samples
PPA022 Increased sensitivity by desalting protein samples
For all prices contact : customersupport@ilifediscoveries.com PPA023 Customized Protein Analysis
Protein Application Other Related Services

A specific chip design allows the analysis of upto 10 protein samples Service Page No.
in the 5-250KDa size range with the use of different kits. A variety Protein Expression & Purification 57
of different samples for can be used such as column fractions, 2D Gel Electrophoresis 60
purified proteins, antibodies and cell lysate. Offgel Fractionation 60
Antibody Microarrays 61
Pathway Focussed Protein Profiling 63
Protein Expression
Analysis of cell lysates - protein induction Biomolecular Intercation Assays using Octet 65
Antibody Production 68
Protein Purification Peptide Synthesis 69
Comparison between lysate and flow through Analysis of protein
purification GFP Streptag fusion protein purification Analysis of Protein Structure Prediction 84
column capacity Analysis of column fractions to optimize conditions Antibody Microarray Data Analysis 85
Protein Expression & Purification

Customized protein expression
Service Information
iLife can help you select expression system and strains, develop At iLife we:
standard protocols, and scale up processes for expression of • Will work with your specifications for quantity, varying size,
proteins from: bioactivity, structure, solubility, post-translational modifications,
compartmentalization (cytoplasmic/periplasmic) and purity, to
Bacterial protein expression systems: They are both the most express proteins that meet your specialized requirements.
universal and the most cost-effective means of protein expression • Will also do small scale induction to over-express target protein
available now-a-days. & carry confirmation test for expressed protein by SDS-PAGE
and western blot (customer must provide appropriate antibodies).
Yeast Expression System: This system is extremely useful for • Amplify/isolate gene of interest out of a customer-supplied vector
expression and analysis of eukaryotic proteins. Protein expression and subclone it into a suitable promoter based high-level
in yeast is genetically well characterized and is known to perform expression vector or a retroviral vector.
many post-translational modifications, grow quickly in defined
medium. It is easy and less expensive to work with, as compared Deliverables :
to insect or mammalian cells & can be adapted to fermentation. 1. Construct(s) report
2. Expression data
Mammalian Expression Systems: These systems ensure 3. Expression positive clones (glycerol stock)
appropriate post-translational modifications (e.g. disulfide bonds, 4. SDS-PAGE
glycosylation, phosphorylation) in case of a mammalian protein and 5. Western Blot data
are therefore, the preferred exression systems in case of mammalian 6. QC data at each step
proteins. It is also required for therapeutics & vaccines production, 7. Project report
hybridoma screening etc.
Ordering Information :
PEP001 Expression and Purification of Protein from Bacteria
PEP002 Expression and Purification of Protein from Yeast
PEP003 Expression and Purification of Protein from Mammalian cells
PROTEOMICS & CELL BIOLOGY Protein Expression & Purification
Epigenetic expression of proteins under Small Scale protein purification for proof
inducible promoters of concept studies
It is quite difficult to express toxic proteins, as they tend to destroy iLife Discoveries is a final solution for your needs, and helps you to
the cell producing them. iLife can help you select the inducible overcome your cloning, protein expression and purification
expression system and strains, develop standard protocols, and bottleneck We offer protein purification services for cDNA cloned
scale up processes for expression of proteins from: into bacterial, mammalian or insect cells. Recombinant protein
production is a core activity of groups involved in high throughput
1. Bacterial protein expression systems screening functional proteins for validation and structural biology.
2. Yeast Expression System The key to achieving efficient and robust purification procedures is
3. Mammalian Expression Systems selection of the most appropriate chromatographic resins for each
separation step comprising a multidimensional process, the
At iLife we sequence of which is critical for optimization of chromatographic
• Besides working on your specifications for the quantity, varying performance in terms of simplicity, scalability and product yield
size, bioactivity, structure, solubility, post-translational
modifications, compartmentalization (cytoplasmic/periplasmic) At iLife we
and purity, to express proteins, we will also work with choice of • Will work with your specifications for quantity, varying size,
inducible vector that meet your specialized requirements for even bioactivity, structure, solubility, post-translational modifications,
Service Information
toxic proteins. compartmentalization (cytoplasmic/periplasmic) and purity, to

• Will also do small scale induction to over-express target protein express proteins that meet your specialized requirements.
& carry confirmation test for expressed protein by SDS-PAGE • Will also do small scale induction to over-express target protein
and western blot (customer must provide appropriate antibodies). & carry confirmation test for expressed protein by SDS-PAGE
• Amplify/isolate gene of interest out of a customer-supplied vector and western blot (customer must provide appropriate antibodies).
and subclone it into an inducible promoter based high-level • Amplify/isolate gene of interest out of a customer-supplied vector
expression vector or a retroviral vector. and subclone it into a suitable promoter based high-level
expression vector or a retroviral vector.
Deliverables:
1. Construct(s) report Deliverables:
2. Expression data 1. Construct(s) report
3. Expression positive clones (glycerol stock) 2. Expression data
4. SDS-PAGE 3. Expression positive clones (glycerol stock)
5. Western Blot data 4. SDS-PAGE
6. QC data at each step 5. Western Blot data
7. Project report 6. Purified soluble tagged/untagged protein in lyophilized form
7. QC data at each step
8. Project report
PEP004 Epigenetic Expression of Protein under inducible
promoters from Bacteria
promoters from Yeast
promoters from Mammalian cells
Protein Expression & Purification

Customized protein purification platforms for Other Related Services
crystallography studies Service Page No.
Protein Analysis 56
At iLife, proteins are usually purified with different columns which 2D Gel Electrophoresis 60
ensure “crystallographic grade” purity. The purity of final products Offgel Fractionation 60
is better than 95% and can be directly used for crystallization and Antibody Microarray 61
Pathway Focussed Protein Profiling 63
other high quality assays. Immunological Assays (ELISA & ELISPOT) 64
Biomolecular Intercation Assays using Octet 65
At iLife we Antibody Production 68
• Will work with your specifications for quantity, varying size, HPLC & LC-MS Services 69
bioactivity, structure, solubility, post-translational modifications, MALDI ToF/ToF Services 70
compartmentalization (cytoplasmic/periplasmic) and purity, to Protein Structure Prediction 84
express proteins that meet your specialized requirements. Antibody Microarray Data Analysis 85
• Will also do small scale induction to over-express target protein
& carry confirmation test for expressed protein by SDS-PAGE
and western blot (customer must provide appropriate antibodies).
• Amplify/isolate gene of interest out of a customer-supplied vector
and subclone it into a suitable promoter based high-level
Service Information
expression vector or a retroviral vector.
Deliverables:
1. Construct(s) report
2. Expression data
3. Expression positive clones (glycerol stock)
4. SDS-PAGE
5. Western Blot data
6. Purified soluble tagged/untagged protein in lyophilized form
7. QC data at each step
8. Project report
PEP010 Purification of 15-20mg protein from Bacteria
PEP011 Purification of 15-20mg protein from Yeast
PEP012 Purification of 15-20mg protein from Mammalian cells
PROTEOMICS & CELL BIOLOGY Protein Profiling
Two - Dimensional Gel Electrophoresis, Offgel Fractionation
followed by MALDI-TOF/TOF
2D-GE is a powerful method, but in case of complex protein
2DGel electrophoresis is the most widely accepted method for mixtures, many times, people are unable to get reproducible
fractionation of complex mixture of proteins into discrete fractionation with high resolution. In contrast to conventional
components. iLife Discoveries offers high-throughput, two- isoelectric focusing, OFFGEL fractionation delivers sample in
dimensional gel electrophoresis for a diverse range of sample types. the liquid phase which is much more convenient for the other
One may like to do 2D gel electrophoresis in various studies like: subsequent separation experiments like liquid chromatography.
The main advantages are that there is no need for gel staining
• Protein Biomarker discovery and no need to recover sample from the gel, which decreases
• Protein species and isoform distribution analysis the loss of sample and increases the reproducibility of the results.
• Analysis of posttranslational modifications The OFFGEL electrophoresis reduces sample complexity,
• Differential protein pattern analysis making it possible to detect a small difference in the protein
• Drug mechanism of action pattern.
• Predictive toxicology
In this technology, proteins are firstly separated according to their

isoelectric point (pI) in the horizontal direction (isoelectric focusing
Service Information
[IEF]) followed by separation in the vertical direction [SDS - PAGE],

according to the molecular weight.
After performing various staining protocols, depending on the

specific sample requirement, spots are obtained on gels, which
correspond to different proteins and are isolated and subjected to
enzymatic digestion for further mass spec analysis. At iLife
Discoveries we do in-gel tryptic digestion (as well as in-solution
digestion for whole proteome) of spots excised from 1D and 2D
gels.
Sample requirements : A combination of OFFGEL fractionation based on isoelectric

• Total protein: > 500ìg/sample, low salt (< 50 mM), without ionic point, and high-sensitivity on-chip electrophoresis with the Agilent
detergents 2100 bioanalyzer, results in an analytical 2D-GE-type analysis
• Cells: > 5x106 cells/sample, 3x washed and centrifuged, 4°C, with excellent sensitivity and reproducibility. Best part of
pellet flash frozen combination of both technologies (OFFGEL and on-chip
• Tissue: >10 mg blood free tissue, washed by PBS, 4°C, flash separations) allows detection of even a small change in protein
frozen pattern.
Deliverables :
• Project Report
POG001 2D Gel Electrophoresis of Bacterial Protein (Low Resolution)
POG002 2D Gel Electrophoresis of Bacterial Protein (High Resolution)
POG003 IEF OffGel Fractionation, for Differential Protein Expression
(Low Resolution)
POG004 IEF OffGel Fractionation, for Differential Protein Expression
(High Resolution)
POG005 IEF OffGel Fractionation, with Digital Analysis of Protein Fractions
(Low Resolution)
POG006 IEF OffGel Fractionation, with Digital Analysis of Protein
Fractions (High Resolution)
POG007 Customized 2D Gel Electrophoresis Services
POG008 Customized OffGel Fractionation Services
Protein Profiling

Antibody Microarray Highlights of the Antibody Array:
The Ab Microarray 500 enables to detect a wide variety of proteins • 507 antibodies printed on one slide (Figure 2).
(both cytosolic and membrane bound) repre-senting a broad range • 380 antibodies printed on one slide.
of biological functions. • Each monoclonal antibody is printed in replicates
The Ab Microarray 380 - Disease Profiling Array enables to detect • Antibodies are covalently immobilized on a standard glass slide
many different proteins that are known to be associated with a (Figure 2)
variety of conditions such as: • Antibodies have high specificity and affinity
• Profiling Disease states • Slides have low background
• Cancer research • Sensitive Cy3/Cy5 fluorescent detection
• Identification of Tumor-associated Antigens • Platform compatible with most commercially available microarray
• Metastasis scanners (75 x 25 x 1 slide)
• Multiple sclerosis
• Parkinson’s
• Alzheimer’s
• Obesity
• Transplanted organ rejection
• Digestive disorders
Service Information
• Time course studies
• Biomarker discovery
• Target validation
• Target Identification
• Serum Analysis in Human, Mouse & Rat Tissues
Examples of the Ab Microarray’s utility include the study of

expression of apoptosis regulatory proteins, as well as the study of
expression of proteins relevant to diabetes. This array allows to
detect differences in protein abundance between two individual
samples—cells, whole tissue, or biological fluids. The fluorescence-
Figure 2. Layout of the Ab Microarray 500. The Ab Microarray 500 contains at
based procedure, which takes less than a day to complete, lets least 500 distinct antibodies arrayed in a 32 x 32 grid on a 75 x 25 x 1 mm glass
you detect as little as 20 pg/ml of each protein target. A dual-color slide (Panel A). Each antibody is printed in duplicate. Panel B. Darker dots at the
detection method is uniquely designed so that inherent variations corners represent Cy3™/Cy5™-labeled bovine serum albumin (BSA) spots, which
in dye labeling do not affect the outcome of the experiment Thus, serve as orientation markers. The open circles correspond to unlabeled BSA spots,
which serve as negative controls. The Ab Array 380 has a similar layout - rows 5
one can be confident that the side-by-side comparisons reflect the and 6 are not printed.
rela-tive abundances of proteins in the sample. Follow-up studies
with Western blotting and in situ hybridization support these claims.
The proprietary process ensures that antibodies remain functionally Procedure
active even after they are covalently immobilized to the glass The antibody array protocol, as followed at iLife Discoveries,
surface. The arrays are printed on 75 x 25 x 1 mm glass slides, all outlined in Figure 3, is a fluorescence-based procedure in which
arrayed antibodies are carefully tested for specificity and sensitivity. antibodies printed on a glass surface are used to capture
Those that display a high degree of cross reactivity are eliminated fluorescently-labeled antigens. The entire procedure, from sample
from the final product. preparation to array scanning, takes one day to complete.
Figure 1: Antibody Microarray Workflow

Figure 3. Flowchart of the antibody array procedure.
PROTEOMICS & CELL BIOLOGY Protein Profiling
Data Validation
The purpose of the antibody array is to provide a reliable, easy-to-
use tool that generates reproducible results. Antibody array results
can be validated by Western blot analysis, ELISA,
immunohistochemistry, or literature searches. The Western blot
analysis and array data show an 80% or better correlation..
We provide complete solution for biomarker discovery starting from

experimental plan, target identification, target validation abd
population screening for the biomarker on Antibody Microarray 500
with salient features.
• We analyze over 500 native proteins in one experiment

• We can compare protein expression levels among cells, tissues,
and biological fluids
• We offer internal normalized results enhancing assay precision
and accuracy
Service Information
• We can detect a wide variety of proteins (both cytosolic and

membrane bound) representing a broad range of biological
functions, including signal transduction, cell-cycle regulation,
gene transcription, and apoptosis.
Customized services include development of microarray with

selected set of antibodies either from the database or customer
owning antibodies.
Our expertise in the technique walks scientist through a complete

project of identifying the antigen in disease states through Ab
microarray and later screen the population through Reverse Phase
Microarray.
Reverse-phase Protein Lysate microarrays (RPAs) provide an
exciting new platform for measuring protein expression levels in a
large number of biological samples simultaneously. Proteomic
profiling using RPAs yields more direct answers to functional and
pharmacological questions than transcriptional profiling.
Data Analysis
Customized data analysis will include identifying the candidate
antibodies showing differential expression and categorizing them
depending on their function.
PAM001 Protein Profiling on Antibody Microarray 500
PAM002 Custom Antibody Microarray
PAM003 Custom Reverse Phase Microarray
Protein Profiling

Pathway Focussed Protein Profiling Ordering Information
Antibody Microarrays provide a high-throughput platform for
Explorer Arrays
sensitive, efficient and accurate protein expression profiling
PPP001 Kinase Antibody Microarray
Population screening, comparison between normal, diseased PPP002 Phospho Explorer Antibody Microarray
or treated samples and identifying candidate biomarkers. PPP003 Tyrosine Phosphorylation Pro Array
PPP004 Protein Explorer Array
Apart from the whole proteome sequencing we also help scientists Phosphorylation Antibody Arrays
screen their clinical samples to explore the disease specific and PPP005 AKT Pathway Phospho-Specific Microarray
PPP006 AMPK Signalling Microarray
pathway specific proteins with our custom made focused antibody PPP007 Cancer/Apoptosis
microarray services. PPP008 Cell Cycle
PPP009 Chromatin/Transcription
Services are available for studying phosphorylation events in cell PPP010 Cytoskeleton
PPP011 EGF Pathway
and tissues and wide range of pathways involved in : PPP012 ERK signalling
• Angiogenesis PPP013 GPCR signalling II
• Cancer markers PPP014 MAPK/ERK
• Cytokines PPP015 Insulin Receptor Pathway
PPP016 GAK/STAT
• Signal Transduction PPP017 MAPK Pathway
• Apoptosis
Service Information
PPP018 Neuroscience
• Cell Cycle PPP019 NF/KB II Signalling
• Hormones PPP020 p53 signalling
PPP021 TGF/beta signalling
• Stem Cell etc. PPP022 Tyrosine Kinase Adaptors
Pathway Antibody Microarrays
Proteins from cell extracts, fresh, frozen and FFPE tissues or serum PPP023 Angiogenesis
samples can be analyzed at our facility with utmost accuracy and PPP024 Cancer Markers
deliver publication ready results. PPP025 Cytokines
PPP026 Signla Transduction
PPP027 Apoptosis
Data Analysis PPP028 Cell Cycle
Customized data analysis will include identifying the candidate PPP029 Hormones
antibodies showing differential expression and categorizing them PPP030 Stem Cells
depending on their function.

Service Page No.
Protein Extraction and Purification 56
Protein Expression & Purification 57
Biomolecular Intercation Assays using Octet 65
Protein Structure Prediction 84
Antibody Microarray Data Analysis 85
PROTEOMICS & CELL BIOLOGY Immunological assays
ELISA ELISPOT
The Enzyme Linked Immunosorbent Assay (ELISA) is a common The Enzyme-linked Immunosorbent SPOT (ELISPOT) is unlike
assay used to check the presence of particular antigens or ELISA where secreted analyte from stimulated cell is captured by
antibodies. labeled antibody for assay.
We offer a wide range of ELISA services which include: Immune cells after interacting antigens secrete number of
• Broad range screening of cytokines, antigens, signaling stimulating chemicals like cytokines. The ability to measure this
molecules, etc. response is vital for drug discovery, basic research and
• Test of various disease related antigens and plant molecules. management of a range of important diseases. This response
• Designed or synthesized peptides/ antigens which can be readout at single-cell resolution is required to develop better
screened against wide range of antibodies understanding and treatment of allergies and autoimmunity, to
predict allograft rejection in organ transplantation, for improvements
Deliverables : in drug and vaccine development, and for monitoring various cancer
1. Project report treatments.
iLife offers a wide range of ELISPOT services which

include :
Service Information
• Broad range screening of cytokines, antigens, signaling molecules, etc.

• Test of various disease related antigens and plant molecules.
• Designed or synthesized peptides/ antigens which can be
screened against wide range of antibodies
Deliverables :
1. Project report

PIM001 ELISA PIM002 ELISPOT
For all prices contact : customersupport@ilifediscoveries.com For all prices contact : customersupport@ilifediscoveries.com
Biomolecular Interaction Assays

iLife offers highly customized assays for investigating biomolecular High Affinity: Carbonic Anhydrase:Azetazolamide (222
interactions. The assays are versatile over a range of interactions Daltons)
including :
1. Antibody-antigen
2. Receptor – Ligand
3. DNA - DNA
4. DNA - Protein
5. Antibody Fragment - Antigen
6. Antibody - Peptide
7. Multiple Antibody Pairings
8. Antibody - Small Molecule
9. Protein - Small Molecule
Figure 3: Analysis of a high affinity interaction (KD= 39 nM): acetazolamide/carbonic
Monitoring antibody concentration anhydrase binding data at 1- 0.012 ìM acetazolamide (3X dilution series) in PBS
iLife is a leading provider to the research and Industrial community with 0.5% DMSO.
of a high-end technology platform for monitoring antibody titres. 1. Papalia et al, Analytical Biochem 359 (2006), 94-105.
On receiving your established hybridomas, we continue to grow 2. Myszka, Analytical Biochem 329 (2004), 316-323.
them in our cell culture facility and monitor the titer values on a
Service Information
regular basis on the Octet Red. Conditions of growth can be altered
as required to be able to achieve larger antibody titre values. Kinetic profiling of immunotherapeutics
Immunotherapeutic agents use or modify immune mechanisms.
Use of these agents is rapidly growing. New classes, new agents,
and new uses of current agents are constantly in the development
pipeline. We can customize experimental protocols to measure on
and off rates of immunotherapeutics. Eight samples can be analyzed
in parallel.
Alternatively popular packages allow for the

quantification of antibody production in Concentration measurements based on binding rates
periodically delivered customer samples Concentration measurements can be made using the binding rates
of test samples which are interpolated from the standard curve.
Small Molecule Detection
Investigating the affinity of a small molecule to bind to a therapeutic Ordering Information
target is a significant step in the drug discovery process. The Octet
RED System is designed to enable the detection of small molecules Service No. Description
using Super Streptavidin Biosensors. Real-time, label-free analysis PBI001 Biomolecular interaction assay for Monitoring
of the association and dissociation of a small molecule with the antibody concentration
target biotinylated protein of interest results in the determination of PBI002 Biomolecular interaction assay for Small Molecule Detection
PBI003 Biomolecular interaction assay for Kinetic profiling of
kinetic constants furnishing pharmacologically relevant data. immunotherapeutics
PBI004 Biomolecular interaction assay for Concentration
measurements based on binding rates
PROTEOMICS & CELL BIOLOGY Biomolecular Interaction Assays
Protein Quantitation in Bioprocessing Kinetic Characterization of Proteins
Our Technologies provide researchers with label-free solution for Kinetic analysis of antibodies and other proteins is critical to both
antibody quantitation. Pre-coated anti-hIgG, anti-murine, and Protein the selection of clones for development and comprehensive kinetic
A Biosensors are ready to use in quantifying a variety of IgG characterization throughout development and production. Using
isotypes. Streptavidin-coated and Amine Reactive Biosensors Octet Red we provide a unique approach in label-free, real-time
expand your quantitation options to include all proteins in developing kinetic analysis streamlining assay development. As a result ka, kd,
custom protein assays. We guarantee accurate results on and KD.
customized workflows resulting on good quality publishable data.
Kinetic Characterization Data
Now you can quantify antibodies or other proteins

that are critical to:
Service Information
• Early cell culture screening

• Protein purification
• Cell line selection for optimization of antibody production
• Biologicals manufacturing monitoring
Accurate antibody quantitation is critical to the selection of cell lines

for development and the optimization of bioreactor titers.
Customised protocols run on this system overcome drawbacks from
traditional measurement methods including those of lack of
specificity, labor-intensive protocols, and variable imprecision. Each
step of bioprocessing, is streamlined hence speeding the workflow
Figure: Kinetic characterization of an antibody to a series of antigen.

Globally fitted curves and residuals
PBI006 Biomolecular interaction assay for Kinetic
characterization of proteins
Figure: Bioreactor process time profile of

cell culture supernatants
PBI005 Biomolecular interaction assay for Protein Quantitation
in Bioprocessing
Biomolecular Interaction Assays

Detection of Anti-Drug Antibodies (ADA) Screen for Drug Inhibitors
Immunogenicity is the degree of the immune response to a Using the biosensor surface within this bio-layer-interferometry
therapeutic drug. Anti-drug antibodies (ADA) produced in the technology platform, it is possible to conduct ligand inhibition assays
process can elicit allergic or anaphylactic reactions, reduction in by expanding the variables that can be measured, such as blocking
efficacy, and/or induction of autoimmunity. Currently a prerequisite or unblocking analytes, affinity, and association and dissociation
for the approval of a therapeutic protein involves strict regulatory rates. Hence screening of large number of drug inhibitors can be
immunogenicity testing of biopharmaceuticals during clinical trials. carried out resulting in the creation of a drug blocking profile.
Using this technology platform we offer the pharmaceutical industry
accelerated detection of anti-drug antibodies (ADA) and
characterization of an anti-drug antibody response.
Service Information
PBI007 Biomolecular interaction assay for Detection PBI008 Biomolecular interaction assay for Screen
of anti-drug antibodies (ADA) for drug inhibitors
For all prices contact : customersupport@ilifediscoveries.com For all prices contact : customersupport@ilifediscoveries.com

Service Page No.
Protein Extraction and Purification 56
Protein Expression & Purification 57
2D Gel Electrophoresis 60
Offgel Fractionation 60
Antibody Microarray Data Analysis 85
PROTEOMICS & CELL BIOLOGY Antibody Production
Monoclonal Antibody Production Polyclonal Antibody Production
iLife provides our customers a very comprehensive expertise in We provide a flexible approach for polyclonal antibody development
the area of Molecular Biology and Immunology giving end-to-end against protein/peptide of choice. The basic service package
solutions for Recombinant Protein and Custom Antibody comprises of antisera development which can be extended to
development. purification trial and final purification which is briefed as follows:
Monoclonal Antibody (Hybridoma) Development in Antisera Development

Mouse • Pre-screening of animals (New Zealand White) against customer-
• Against a customer-supplied protein or peptide supplied/synthesized immunogen and selection of animals for
• Delivery of best-reacting clones and/or continuous supply of immunization
Purified Antibody • Conjugation of immunogen to carrier proteins if immunogen is
• We can also design and synthesize the peptide (immunogen) less than 10 KDa.
• Primary Immunization of animals
• Secondary immunizations (boosters) and titer checks (maximum
The service package for monoclonal Ab production of 4 rounds)
can be inclusive of : • Production bleed from 2nd booster onwards after satisfactory
• Pre-Screening of Mice against customer supplied/synthesized titer (see notes below) is achieved
Immunogen • Termination and exsanguination bleed after 3rd or 4th booster
Service Information
• Antigen conjugation with carrier protein (depending on titer value)

• Primary immunization
• Affinity maturation Trial Purification
• Fusion of splenocytes with myolema cells • Protein A purification of Antisera or
• Colony selection and screening on ELISA • Antigen-Affinity purification of Antisera
• Final selection • Quality analysis of purified antibody samples by Affinity check
• Single-Cell Cloning with Antigen-Dilution ELISA & Specificity check with Western
• Mass Culture and Antibody Purification Blot (for proteins or large peptides only)
• Quality Analysis of Purified Antibody with Western Blotting
Final Purification
• Column preparation and Purification
• Dialysis and concentration of antibody
• Quality analysis of purified antibody
• Affinity check: Antigen-Dilution ELISA
• Specificity check: Western Blot (for proteins or large
peptides only)

Service Page No.
PAD001 Monoclonal Antibody production
PAD002 Polyclonal Antibody Production
Peptide Synthesis Metabolite Analysis

Custom Peptide Synthesis HPLC & LC- MS Services
Our peptide synthesis service uses both Solid Phase and Solution FOOD & AGRICULTURE
Phase technologies under cGMP conditions. The high quality
Food and agro based products can be assessed for nutritional
peptide synthesis service guarantees to meet or exceed customer
labeling, food safety evaluations, trace analysis and shelf life studies.
specifications with following peptide features:
Products tested :
• Peptide length: upto 120 AA
• Fruits & vegetables (afresh, concentrates, even processed)
• Peptide Purity: From Crude to 99%
• Beverages
• Quantities: from 1mg to Kilograms
• Milk & milk products
• Delivery: ~3 weeks
• Water
• Sulphated/Phosphorylated Peptides
• Spices
• Multiple Disulfide bridges
• Cereals & Pulses
• Chromogenic/ Fuorogenic Substrates
• Baked products
• Special Amino acids & derivatives
• Meat & meat products
• Peptide protein Conjugation
• Egg & egg powder
• Peptide modifications with C-terminal Biotin, AMC, Aldehyde, CMK
Expertise :
Service Information
All the peptides will be delivered with a Certificate of Analysis
specifying the HPLC gradient used to determine peptide purity. • Nutrient profiling
• Amino acid profiling
We specialize in supplying 20 to 1000 peptides with lengths up to • Water soluble vitamins by HPLC
15 residues and quantities up to 30 mg. We also offer ~1mg of • Fat soluble vitamins by HPLC
peptide on 96 well plates. For large peptide synthesis projects, we • Food additives
also offer peptide pooling. • Acidulants
• Antioxidants
• Preservatives
• Artificial Sweeteners
• Synthetic colors
• Nutritional labeling
• Trace analysis
• Pesticide residues
• Antibiotic residues
• Dioxins
• Toxic metals
• Mycotoxins
• Epoxy resins
• Chemotherapeutics and antiparasitic agents
• Microbiological evaluations
• Shelf life studies
DRUG & PHARMA

Ordering Information Method Development and Validation
We offer analytical method development and validation services
Service No. Description for batch to batch uniformity, identification & quantification of
PAD003 Custom Peptide synthesis impurities or Active Pharmaceutical Ingredient (API) in formulations
and other departments involved in day to day QA/QC or R&D works
For all prices contact : customersupport@ilifediscoveries.com in the biopharma industry.
Method development is undertaken for:
Other Related Services • Assay
• Dissolution
Service Page No. • Purity and concentration
Protein Analysis 56 • Trace metals
Immunological Assays (ELISA & ELISPOT) 64 • Residual solvents
Biomolecular Intercation Assays using Octet 65 • Physico-chemical tests
Antibody Production 68 • Structural confirmation
• Metabolite monitoring
• Half life in the body
PROTEOMICS & CELL BIOLOGY Metabolite Analysis
MALDI ToF/ToF Services
iLife is equipped with the next-generation tandem time-of-flight MS/
MS system - ABI4800 MALDI ToF/ToF Analyzer.
Peptide Mass Fingerprint (PMF) –

• Trypsinization of Protein sample
• Protein Mass Fingerprinting using MALDI ToF
• Protein identification using MASCOT protein database.
MS/MS peptide fragmentation –
• Automated spot Picker is used for the precise excision of protein

of interest for further proteomic analysis.
• Trypsinization of Protein sample
• Protein Mass Fingerprinting using MALDI ToF
• Protein identification using MASCOT protein database.
WATER Analysis
Mass Analysis- by MALDI ToF for proteins/peptides
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iLife the quality of water is assessed for various applications as

mentioned below: de novo sequencing of proteins/ peptides
• Biomedical Grade water
• Electronics Grade water
• Water for Food processing Industry
• Reagent water
• Water for storage batteries
• Water for fermentation industry
• Water for Ice manufacture
• Water for Tanning Industry
• Brine solutions for trace metals.
• Feed water used for animals in safety data generation
• Cooling tower water for physico-chemical tests and Legionella.
• Trace metal analysis.
PMD009 Trypsinization
PMD010 Mass Analysis of Proteins
PMD011 Mass Analysis of Peptides
PMD012 Peptide Mass Fingerprinting through MALDI ToF
PMD013 MALDI ToF/ToF
PMD014 De novo sequencing by MS/MS
Service No. Description For all prices contact : customersupport@ilifediscoveries.com
PHP001 Analytical HPLC
PHP002 Semi-preperative HPLC
PHP003 Perparative HPLC Other Related Services
PHP004 Customized LC-MS/MS solutions
PHP005 Customized GC-MS Service Page No.
PHP006 Customized GC-MS/MS
PHP007 Customized GC-MS/TOF Protein Extraction and Purification 56
PHP008 Customized ICP MS Protein Expression & Purification 57
2D Gel Electrophoresis 60
Offgel Fractionation 60
For all prices contact : customersupport@ilifediscoveries.com Protein Structure Prediction 84
Customized Cell Biology Services

Adenovirus Services Generation of Stably Expressing Cell Lines
In order to address the complex issue of protein expression in variety We offer advanced level of research support services in generating
of cell lines and biological systems, Our team of scientists have cell lines from different mammalian and non mammalian sources
developed capabilities for Recombinant adenoviruses based for the research use. The cell lines are generally created with
epigenetic gene transfer technology and corresponding protein integration of plasmid DNA of choice to create cell lines expressing
expression. recombinant clones.
Our service is an integrated model where our team of cell biologists
works actively in coordination with the team of bioinformatics
experts, molecular biologists and proteomics specialists. We take
up stable cell line construction at its entirety where we design,
amplify clone and express the gene of interest in the first phase.
Special care taken to select the vectors for expression of the gene
of interest. We have a large repository of commercially available
and custom tailored vectors for integrating plasmid DNA constructs
encoding dual antibiotics resistance gene to ensure highly specific
clonal selection.
Service Information
Our services include
1. Design of the entire project for stable cell construction
2. cDNA synthesis to expression of the protein in a mammalian
cell compatible vector with dual antibiotic resistance genes
3. Selection of the right cell line
4. Initial transient transfection study for the verification
5. Stable cell line transfection under CLASS-10000 clean room
conditions
6. Selection of multiple clones from the transfected population
7. Differential gene expression studies of the stable cell lines to
study the effect of over expression of gene of interest.
8. Constitutively active expression based recombinant cell lines
9. Stable cell lines with integrated inducible expression systems
for the study of toxic or pro-apoptotic genes.
We offer Continuous supply of viral particle preparations which are
ready to infect the cells of your interest once the viral particle
construction is completed, there by eliminating the need to set up
infrastructures in your laboratory.
We offer complete services ranging from design of different Novel Cell Lines Development
adenoviral expression platforms. Our custom services include the As an innovative new addition to our
overall evaluation of project, analysis of the primary and/or capabilities, our cell biology team can
transformed cell lines for which the expression systems are created develop novel cell lines from a variety
etc. We internally design appropriate primers for amplifying the of mammalian and non mammalian
ORF (Open Reading Frame), construction of wild type ORF with sources. We are currently developing
N- or C- terminal tags for sub-cellular localization and/or purification, cell lines of pharmaceutical relevance.
creation of library of mutant proteins etc prior to their integration These cell lines are derived from
into the appropriate adenoviral backbone. Indian population with different
Small or big ORFs: Expression in hard to transfect cells lines; cultural and dietary practices.
Expression for large scale purification of proteins; Expression for
studying sub-cellular localization, adenovirus are the most accepted
ways for expression recombinant proteins with highest efficiency Ordering Information
with least undesirable effects.
Our adenoviral expression services are performed on a customized Service No. Description
project format. PCB001 Adenovirus Services
PCB002 Generation of Stably Expressing Cell Lines
PCB003 Novel Cell Lines Development
PCB004 Other Customized Cell Biology Services
BIOINFORMATICS
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BIOINFORMATICS
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Bioinformatics
Simplify Complex Life Research
Bioinformatics Expertise
Introduction and Applications
Bioinformatics or computational biology is the use of mathematical Pharmacogenomics
and informational techniques, including statistics, to solve biological Pharmacogenomics is the application of genomic approaches and
BIOINFORMATICS
problems, usually by creating or using computer programs, technologies to the identification of drug targets. In Short,
mathematical models or both. One of the main areas of pharmacogenomics is using genetic information to predict whether
bioinformatics is the data mining and analysis of the data gathered a drug will help make a patient well or sick. It Studies how genes
by the various genome projects. Other areas are sequence influence the response of humans to drugs, from the population to
alignment, protein structure prediction, systems biology, protein- the molecular level.
protein interactions and virtual evolution.
Pharmacogenetics
Genomics
Pharmacogenetics is the study of how the actions of and reactions
Genomics is any attempt to analyze or compare the entire genetic
to drugs vary with the patient’s genes. All individuals respond
complement of a species. It is, of course possible to compare differently to drug treatments; some positively, others with little
genomes by comparing more-or-less representative subsets of obvious change in their conditions and yet others with side effects
genes within genomes. or allergic reactions. Much of this variation is known to have a genetic
basis. Pharmacogenetics is a subset of pharmacogenomics which
Comparative genomics uses genomic/bioinformatic methods to identify genomic correlates,
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The study of human genetics by comparisons with model organisms for example SNPs (Single Nucleotide Polymorphisms),
such as mice, the fruit fly, and the bacterium E. coli. characteristic of particular patient response profiles and use those
markers to inform the administration and development of therapies.
Strikingly such approaches have been used to “resurrect” drugs
Structural genomics or structural thought previously to be ineffective, but subsequently found to work
bioinformatics with in subset of patients or in optimizing the doses of chemotherapy
This refers to the analysis of macromolecular structure particularly for particular patients.
proteins, using computational tools and theoretical frameworks.
One of the goals of structural genomics is the extension of idea of Cheminformatics
genomics, to obtain accurate three-dimensional structural models The mixing of those information resources to transform data into
for all known protein families, protein domains or protein folds. information and information into knowledge for the intended purpose
Structural alignment is a tool of structural genomics. of making better decisions faster in the arena of drug lead
identification and optimization. Related terms of Cheminformatics
Functional genomics are chemi-informatics, chemometrics, computational chemistry,
chemical informatics, chemical information management/science,
Functional genomics is a field of molecular biology that is attempting and Cheminformatics.
to make use of the vast wealth of data produced by genome
sequencing projects to describe genome function. Functional Biomedical Informatics / Medical Informatics
genomics uses high-throuput techniques like DNA microarrays,
proteomics, metabolomics and mutation analysis to describe the Biomedical Informatics is an emerging discipline that has been
function and interactions of genes. defined as the study, invention, and implementation of structures
and algorithms to improve communication, understanding and
Proteomics management of medical information.
Proteomics is the study of proteins - their location, structure and

function. It is the identification, characterization and quantification
Computational Chemistry
of all proteins involved in a particular pathway, organelle, cell, tissue, Computational chemistry is the branch of theoretical chemistry
organ or organism that can be studied in concert to provide accurate whose major goals are to create efficient computer programs that
and comprehensive data about that system. calculate the properties of molecules (such as total energy, dipole
moment, vibrational frequencies) and to apply these programs to
Proteomics is the study of the function of all expressed proteins. concrete chemical objects. It is also sometimes used to cover the
The study of the proteome, called proteomics, now evokes not areas of overlap between computer science and chemistry.
only all the proteins in any given cell, but also the set of all protein
isoforms and modifications, the interactions between them, the
structural description of proteins and their higher-order complexes,
and for that matter almost everything ‘post-genomic’.
Bioinformatics Expertise
In silico Drug Designing Agroinformatics / Agricultural Informatics
Drug discovery is a complex and risky treasure hunt to find the Agroinformatics concentrates on the aspects of bioinformatics
most efficacious molecule which do not have toxic effects but at dealing with study of plant genomes. Microarray techniques to study
BIOINFORMATICS
the same time have desired pharmacokinetic profile. The hunt starts plant disease and causing factor for it due to which genes are
when the researchers look for the binding affinity of the molecule suppressed or over expressed. Comparative genome analysis of
to its target. Huge amount of research requires to be done to come different species of plants.
out with a molecule which has the reliable binding profile. Once the
molecules have been identified, as per the traditional methodologies, Systems Biology
the molecule is further subjected to optimization with the aim of
improving efficacy. The molecules which show better binding are Systems biology is the coordinated study of biological systems by
then evaluated for its toxicity and pharmacokinetic profiles. It is at investigating the components of cellular networks and their
this stage that most of the candidates fail in the race to become a interactions, by applying experimental high-throughput and whole-
successful drug. Bioinformatics make it easier to design successful genome techniques, and integrating computational methods with
drug with the help of bioinformatics tools and also saves time and experimental efforts.
money.
Service Information
Microarray Data Analysis
Microarray data sets are very large and complex. Statistical challenges include taking into account effects of background noise and
appropriate normalization of the data. Normalization methods are different for different platforms.
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Data Processing
This include background subtraction (global or local background), determination of spot intensities and intensity ratios, visualization of
data in different plots and log-transformation of ratios, global or local normalization of signal intensity values.
Statistical Analysis for Significant Changes

This include paired t-test, unpaired t-test, -Test against 0, Welch t-test, Unpaired Mann-Whitney Test, The Paired Mann-Whitney Test,
One-Way ANOVA, two-way ANOVA, Multi-Way ANOVA, Friedman Test, Kruskal-Wallis Test, multiple testing correction, Clustering
(Hierarchical, K-Means, SOM). These methods assess statistical power based on the variation present in the data and the number of
experimental replicates, and can help in minimizing errors in the analysis.
Biological Contextualization of Data

This includes GO analysis, GSEA, Class Prediction, Pathway analysis etc.
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Single Color and Two Color Gene Expression Data Analysis
A gene expression microarray is a technology used in genomics to Custom designed array etc. Please contact us and ask what you
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know the change in expression of gene. microarray has thousands could get from your experimental data after analysis.
of spots of DNA oligonucleotides, that is called features. Each
feature contains several probes of a specific DNA sequence of Basic Analysis
particular gene. Probes hybridize to cDNA or cRNA sample and is • Data processing
detected and quantified to determine relative abundance of cDNA • Normalization
or cRNA in the sample. One micro array chip contains thousands • Experiment Grouping
of probes, a microarray experiment can perform many genetic tests • Quality control on samples
in parallel. This is why microarray is the first choice of any genetic • Quality control on probes
research. • Statistical Analysis
Due to biological complexity of gene expression, data analysis plays • Differentially expressed significant genes
major role to find out differentially expressed genes. To begin with • Fold change analysis
data analysis, experimental design is very critical and important if • Clustering of genes
statistically and biologically significant conclusions are to be drawn • PCA Analysis
from the data. Hence at least two biological or technical replicates
are important for analysis. Advance Analysis
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• GO Analysis
Under this section we can analyze any platform based gene • GSEA
expression data analysis such as Agilent single color and two color, • Class prediction
Affymetrix single color, Illumina single color and two color, • Pathway Analysis
Nimblegen single color, GE single color, combimetrix single color
and all kinds of custom array for single color and two color.
Agilent Single Color or Two Color Microarray

Data Analysis
Send us only .txt file which has been extracted from FE software,
mention the technology name you worked with. We would do whole
data analysis and give you differentially expressed genes and
pathway associated with that.
Affymetrix Microarray Data Analysis

Provide us .CEL or .CHP file and relax. We would analyze your
experimental data and would give you valuable biological
significance of your data.
Illumina Microarray Data Analysis

Send us .txt file which has been extracted from bead studio. We
would analyse your experimental data and would give your result
which would be very much biological significant.
Different File Format

If your experimental data is in another file format such as .xls, tab
delimited etc and is processed then also you could send us your
data for further analysis but in this case please write in detail that Ordering Information
how did you process your data and what algorithms you are
interested in? we only need intensity value and any gene identifier
BOC001 One Color microarray Basic Data Analysis
for data analysis. We would analyze your experimental data with
BOC002 One Color Microarray Advance Data nalysis
your given specification and would provide you significant result. BTC003 Two Color Microarray Basic Data Analysis
BTC004 Two Color Microarray Advance Data Analysis
Other Microarray Platform For all prices contact : techsupport@ilsbioservices.com

We could analyze any microarray data which might have come
from any microarray platform like GE, Nimblegen, Combimetrix,
Exon Expression and Exon Splicing Data Analysis
Alternative splicing is defined as variations in RNA splicing Advance Analysis
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mechanisms resulting in multiple splice variants. Affymetrix Exon • GO analysis

array is used for studying the alternative splicing of genes which is • GSEA
important in identifying the antagonistic functions, certain genetic • Class prediction
mutations which result in human diseases and understanding the • Pathway analysis
disease mechanism or biological process.
Multiple probes per exon enable “exon-level” analysis and allow us
to distinguish between different isoforms of a gene. This exon-level
analysis on a whole-genome scale opens the door to detecting
specific alterations in exon usage that may play a central role in
disease mechanism and etiology.
The second level is “gene-level” expression analysis, in which
multiple probes on different exons are summarized into an
expression value of all transcripts from the same gene.
Exon arrays provide the most comprehensive coverage of the
genome, including empirically supported and predicted transcribed
Service Information
sequences, enabling the discovery of previously unidentified novel

events.
Basic Analysis
• Data processing
• Normalization
• Experiment Grouping
• Quality control on samples
• Quality control on probes
• Statistical analysis
• Differentially expressed significant genes
• Filter Transcripts on DABG
• Splicing ANOVA
• Filter on Splicing Index
• Splicing Visualizations
• Profile Plot
• Variance Plot
• Fold change analysis
• Clustering of genes
• PCA analysis
BEE001 Exon Expression Basic Data Analysis
BEE002 Exon Expression Advance Data Analysis
BES003 Exon Splicing Basic Data Analysis
BES004 Exon Splicing Advance Data Analysis
For all prices contact : techsupport@ilsbioservices.com
Copy Number Analysis Association Data Analysis
Copy number analysis is important to understand or to explore the Genome wide association study (GWAS) is extremely significant
BIOINFORMATICS
effect of copy number variation in human disease and point method to identify susceptible genes involved in common human
mutation. Ideally there would be two copies of gene in human diseases and increases chance to find targets for therapeutic
genome but practically we see that there is several copy of same intervention that act on the root cause of disease.
gene present in genome which trigger disease or prevent disease This analysis requires thousands of case control sample to find
in human. Several thousands of SNPs can be estimated for the statistically significant loci which occurs due to difference in allele
copy number variation and genomic position. or genotype frequency in case with respect to control. The difference
in allele or genotype frequency can lead to disease and thus this
This analysis includes Copy Number Variants , Copy Number method is useful in disease gene discovery due to unbiased study
Polymorphism, Log Ratio, Loss in Heterozygosity (LOH), of gene with known or suspected disease mechanism.
cytogenetic analysis, genotyping calls, copy number calls for CNV
regions and individual probe sets, cluster graphs, Reference analysis Analysis includes
against control, Allele Specific Copy Number, Parent specific Copy • Quality control on samples
Number, Genomic Identification of Significant Targets in Cancer • Filter to get significant SNP
(GISTIC) etc can be analyzed. • PCA
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Copy number analysis • Pearson’s chi square test
• Quality control on samples • Fisher’s exact test
• Batch Effect Correction • Cochran-Armitage test
• Statistical t-test • Regression analysis
• PCA • Linkage Disequilibrium (LD) analysis
• Mean log ratio
• Filtering
• GISTIC
• Clustering
• GO analysis
BCN001 Copy number Analysis
BAA002 Association Data Analysis
miRNA Data Analysis Custom Chip Design and Analysis
miRNA is small single-stranded, regulatory RNA molecules of To study gene expression at genomic scale mostly microarray chips
BIOINFORMATICS
approximately 25 nuceotide base long molecule. miRNA regulates are, it is important to design oligo probes that specifically represent
mRNA by binding with complementary sequences of mRNA and large number of DNA sequences. Cross-hybridization is most likely
affects the expression of genes involved in several biological from the regions with contiguously and perfectly matched bases.
processes. Therefore miRNA expression study became important Oligo probes should also be sensitive and hybridize under similar
class to understand the role of miRNA in regulating biological conditions. We design chip for diagnostic purposes by picking
processes. miRNA array developed to study expression patterns specific oligos by skipping regions with contiguous bases common
of miRNA for any conditions and can be correlated with their target in other sequences. We assure for specificity and it is double-
gene expression studies. checked by NCBI BLAST. Similar sequence regions to non-coding
RNAs are avoided because total RNA are often used for array
hybridization. Low-complexity regions are also filtered out to
Basic Analysis maintain oligo specificity. Oligos and sequence regions that may
• Data processing form secondary structures are discarded since both the probes and
• Normalization the sequence target sites should be easily accessible for
• Experiment Grouping hybridization.
Parameters are taken in consideration while primer
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• Differentially expressed significant genes designing:
• Fold change analysis Probe Copy, Probe Length, Tm range, Cross-reactivity, BLAST
• Clustering of genes score, End preference, Filter file, Cross-reactivity to filter sequences,
• PCA analysis Cross-reacting probes.
We provide services for custom chip design for microarray
Advance Analysis experiemnt. Custom chip design could be done on user’s
• Find target gene (Target Scan) preferences. Selected probes or genes of user’s interest can be
• GO analysis spotted on glass slide to carry out higher level of research.
• GSEA This is also like another microarray chip but only difference would
• Class prediction be in quantity and specificity of genes, for certain experiment.
• Pathway analysis Analysis of data would be same as per other microarray experiment.
Basic Analysis
• Normalization
• Differentially expressed
significant genes
• PCA analysis
Advance Analysis
• GO analysis
• GSEA
• Class prediction
• Pathway analysis

BMR001 miRNA microarray Basic Data Analysis BOP001 Other platform Basic Data Analysis
BMR002 miRNA microarray Advance Data Analysis BOP002 Other platform Advance Data Analysis
BCC003 Chip-on-chip Data Analysis
For all prices contact : techsupport@ilsbioservices.com BDM004 DNA Methylation Data Analysis
BCD005 Custom Chip Design
RT PCR Data Analysis
Real Time PCR is used to measure the quantity of DNA, cDNA, or
RNA present in a sample. It is extremely sensitive technique for
mRNA detection and quantization. RT-PCR can be used to quantify
BIOINFORMATICS
mRNA levels from very smaller samples. Therefore, this technique
is very sensitive to enable quantitation of RNA from a single cell. A
DNA-binding dye binds to all double-stranded DNA in PCR that
causes fluorescence. An increase in DNA product during PCR leads
to an increase in fluorescence intensity and is measured at each
cycle, this allows DNA concentrations to be quantified. However,
DNA dyes bind to all DNA PCR products, including nonspecific
PCR products like Primer, dimer. This can potentially interfere with
or prevent accurate quantification of the intended target sequence.
Basic Analysis
• Data processing
• Normalization
Service Information
• Differentially expressed significant genes
• PCA analysis
Advance Analysis
• GO analysis
• GSEA
• Class prediction
• Pathway analysis
BRT001 RT PCR Basic Data Analysis
BRT002 RT PCR Advance Data Analysis
Comparative Genomics
The comparative genome analysis is done to establish the
correspondence between genes or other genomic features in
different organisms. We can create intergenomic maps that make
BIOINFORMATICS
it possible to trace the evolutionary processes responsible for the

divergence of two genomes. At the lowest level, point mutations
affect individual nucleotides. At a higher level, large chromosomal
segments undergo duplication, lateral transfer, inversion,
transposition, deletion and insertion. Many of these studies are
based on the homology detection and protein families computation.
Biological Systems Modeling

This involves the use of computer simulations of the networks of
metabolites and enzymes which comprise metabolism, signal
transduction pathways and gene regulatory networks to analyze
and visualize the complex connections of these cellular processes.
Sequence Analysis
• Determination genes that encode proteins, RNA, regulatory
Service Information
sequences, structural motifs, and repetitive sequences.

• Intra species and inter species gene comparison to know
similarities between protein functions, or relations between
species.
• High throughput genome sequence analysis for inter-related,
non-identical overlapping sequences of closely related varities
• Whole Genome reconstruction.
Evolutionary Studies
• Tracing the evolution of an organism based on the changes in
its DNA contents.
• Construction of complex evolutionary computational models.
Gene Annotation Ordering Information

• Target Gene finding to search for protein-coding genes and other Service No. Description
functional sequences within a genome. BGW001 Genomics Work in Bioinformatics
Comparative Genomics
Primer Designing
PCR is a common technology applied in the biomedical and
BIOINFORMATICS
biotechnology field. This technology is used for fast mass duplication
of DNA sequences. By repeating some cycles of the denaturation,
annealing and extension reactions, it allows a small amount of DNA
to be amplified exponentially. Typically 25–45 of these cycles are
performed. Amplification of specific regions of the genome is
determined by specific primer sets with forward and reverse
orientation. Typically considered primer design constraints are the
length of the primer, the GC content, the melting temperature and
GC clamp. The length of primers should be within 16 bps~28 bps,
while the difference of the primer pair length should not exceed 3
bps. The GC content of primers should be within 40%~60%. The
melting temperature of primers should be within the range of
o o
50 C~62 C, while the difference of the melting temperature of a
o
primer pair should not exceed more than 5 C. Finally, the 3’ end of
primers should be G or C.In recent years, secondary structures
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were applied to primer design, such as dimer, hairpin and specificity
of a primer pair. Use dynamic programming to design primers. This
allows for designing multiple primers for multiple target DNA
sequences. We identify the specificity-determining subsequence
of each primer and examine its uniqueness in the target genome.
BPD001 Primer Designing
BPW002 Proteomics Work in Bioinformatics
Prediction of Protein Structure
Protein-Protein Interaction Studies Peptide Library Synthesis
Protein is made up of amino acids and the information for protein We could generate peptide of your interest from the given protein.
BIOINFORMATICS
synthesis (primary structure) during translation comes from codon. We could help you in peptide array design to study the specific
Codon is the three nucleotide sequence on gene that is why we protein of your interest. Peptide library is a systematic combination
could determine primary structure of protein from the gene of any of different peptides in large number. It is widely used in :
species. It is vital to know the structure of protein (secondary, tertiary • Drug screening
and quaternary) which may cause disease or prevent the disease. • Target validation
• Epitope mapping
Bioinformatics is used for the study of homology modeling to predict • Vaccine development
the function of a gene with unknown function. if a gene X with • Peptide biological assay
known function is homologous to the sequence of gene Y whose • Protein-peptide binding assay
function is not known, but we could conclude that while gene X and • Immune monitoring
Gene Y share same genetic information then function of gene Y • Structure-activity studies.
could be the same as function of gene X. In the structural
bioinformatics, homology is used to determine which parts of a
protein are important in structure formation and interaction with
other proteins and this technique is called homology modeling.
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With the help of bioinformatics tool we could predict protein-protein

interaction to identify and catalog physical interactions between
pairs or groups of proteins. Protein–protein interactions is important
for the investigation of intracellular signaling pathways, modeling
of protein complex structures and for gaining insights into various
biochemical processes.
• Prediction of co-evolved protein pairs based on similar
phylogenetic trees
• Identification of homologous interacting pairs
• Identification of structural patterns
• Network visualization of protein–protein interactions

BPA001 Peptide Array Design BPL001 Peptide Library Synthesis
BPP002 Protein-Protein Interaction
Antibody Microarray Data Analysis
The advanced step of microarray technique wherein researchers
concentrate on small number of proteins and their role in biological
system. Antibody microarray technique is used to discover and
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validate protein biomarkers in different samples. This is also used
as screening techinique for biomarker discovery and rapid
expression profiling of proteins.
Antibody microarray experiment is inexpensive for throughput

analysis of proteomic profile which allows researchers to focus on
a small number of proteins that have biological relevance.
Antibody microarray data analysis includes :

• Data processing
• Dye swapping
• Global Normalization
• Local Normalization
• Quality control on entities
• Filter on entities
Service Information
• Clustering analysis
• PCA analysis
• Data visualization in different plot
• Scatter plot
• Profile plot
• Box whisker plot Ordering Information
• Histogram
• MvA plot
BAM001 Data Analysis for antibody microarray 500
• PCA plot BAM002 Data Analysis for pathway focused antibody
microarray
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Shipment Guidelines
For Protein Applications
Sample Type Sample Shipment Guidelines
Tissue Transport it in Frozen Condition i.e. Dry Ice

Cell lysate Lyophilized Form(Preferred) or ship it in Frozen condition (Dry Ice)
Culture Supernatant Lyophilized Form(Preferred) or ship it in Frozen condition (Dry Ice)
Blood Plasma Transport it in Frozen Condition i.e. Dry Ice
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Body Fluids Lyophilized Form(Preferred) or ship it in Frozen condition (Dry Ice)
For RNA Applications

RNA Precipitate in sodium acetate & transport it in dry ice/liquid nitrogen

Animal Tissue Suspend in RNA Later & transport it in dry ice/liquid nitrogen
OR
Directly freeze the tissue & send it in dry ice/liquid nitrogen
Plant Tissue Crushed and suspended in RNA Later & send it in dry ice/liquid nitrogen
OR
Directly freeze the tissue & send it in dry ice/liquid nitrogen
Cells Peletted, suspended in RNA Later & transport it in dry ice or liquid nitrogen
Blood Collect in heparin coated vaccutainers & transport it at 4 degrees (gel packs)
For DNA Applications

DNA In Dry ice/Liquid nitrogen
Animal Tissue Suspend in Ethanol & transport it in dry ice/liquid nitrogen
Plant Tissue Suspend in Ethanol & transport it in dry ice/liquid nitrogen
Cells Peletted and freezed & transport it in dry ice/liquid nitrogen
Blood Collect in heparin coated vaccutainers & transport it at 4 degrees (gel packs)
Terms & Conditions Ordering Information
PRICES U.S., CANADA & EUROPE ORDERING
• Please contact our Customer Support Team
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(customersupport@ilifediscoveries.com) to obtain a quote for By Mail: Imperial Life Sciences USA, Inc.
the desired published services. 1630-1634, Centre Pointe Drive, Milipitas
• Please provide the Service no. and Quantity of the samples for CA, 95035- USA
desired services, when contacting our Customer Support Team.
• Customized quotes can be provided based on various factors By Telephone: 9:30 AM to 7:00 PM IST
+1 408- 907 6645
such as nature & duration of the project. Please contact our
Customer Support Team (customerupport@ilifediscoveries.com)
By Email: customersupport@ilifediscoveries.com
for customized quotes.
NOTE: All customized projects need additional background By Internet: http://www.ilifediscoveries.com
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to be exercised with Price list under Rate Contract. Please
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storage at customer site, iLife Discoveries does not guarantee NOTE: All Orders are to be placed in the name of
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should be made irrespective of data generated.
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expressly agreed to in written by a duly authorized officer of iLife.
• Request for storage of samples is agreeable with an additional cost..
• Request for return of samples will not be accepted.
High-end Technology Training
BIOINFORMATICS
Microarray technology Agilent 2100 Bioanalyzer

Training on the Agilent microarray system includes: Training on Bioanalyzer includes:
Introduction to the microarray technology Introduction to Bioanalyzer technology

Custom microarray designing Qualitative and Quantitative Analysis of DNA
Microarray types & applications Qualitative and Quantitative Analysis of RNA(RIN number)
Experimental workflow including Qualitative and Quantitative Analysis of Protein
Gene Expression microarray experimental runs Troubleshooting
Duration: 10 working days Duration: 3 working days
Service Information

GHT001 Microarray Technology Training (Academic)
GHT002 Quantitative PCR Technology Training (Academic)
GHT003 Agilent 2100 Bioanalyzer Technology Training (Academic)
GHT004 Microarray Technology Training (Corporate)
GHT005 Quantitative PCR Technology Training (Corporate)
GHT006 Agilent 2100 Bioanalyzer Technology Training (Corporate)
GHT007 Customized High end Technology Training
Quantitative PCR technology
Training on Quantitative PCR includes:
Introduction to the QPCR technology

Experimental workflow
Primer/ Probe designing, synthesis and validation studies
Duration: 3 working days
forteBio Octet Technology Training
BIOINFORMATICS
Training on Octet includes
Introduction to Biolayer Interferometry Service No. Description
technology and Instrumentation PHT001 ForteBio Octet Technology Trainig (Academia)
Protein Quantitation studies PHT002 ForteBio Octet Technology Trainig (Corporate)
Kinetic Characterization studies PHT003 Customized Octet Technology Training (Academia)
Small molecule and small peptide studies
Duration: 4 working days
Service Information

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The iLife Advantage

Training and Expertise Development

“Seamless and real time access to multitudes of

Corporate Floor at iLife Discoveries

If you need immediate assistance,

• Identification of genetic pathways important for

• Species level identification using molecular techniques

Food and Nutrition Science

• High throughput screening for desired pathways/disease • Antibody arrays

Infectious Diseases Clinical Proteomics

COSMODEx: Cosmeceutical Discovery using

Application of t-TRAP Technology for Determining Food Safety

PRIME-C : Pharmaceutically relevant Repository of Intelligent

Every research lab has its own requirement. We

Customized protein purification platforms for

DNA Extraction & Purification Plasmid DNA Purification

RNA Extraction & Purification

For all prices contact : customersupport@ilifediscoveries.com

Other Related Services

Agilent 2100 Bioanalyzer – Technical Note

Applications for a Lab-on-a-Chip Technology

Restriction digest analysis Ordering Information

Forensic testing RNA Application

Analysis of total RNA Cell Fluorescence Analysis

Analysis of T7-RNA transcripts

Restriction Fragment Length Polymorphism (RFLP) Analysis

Figure : Electropherogram of RFLP on Agilent 2100 Bioanalyzer

Fragment Analysis for RFLP

Different kits allows to automatically size and quantitate restriction

DNA 1000 Kit Figure : RFLP representation on Agilent 2100 Bioanalyzer

For all prices contact : customersupport@ilifediscoveries.com

DNA 1000 Kit For all prices contact : customersupport@ilifediscoveries.com

DNA 7500 and DNA 12000 kits

Custom PCR Assays PCR Analysis

Sample Submission Requirement:

Primer Design, Synthesis & Validation

Synthesis of Forward and

Other Related Services

Custom cloning service includes designing and synthesis of primer,

Precautions and limitations:

For all prices contact : customersupport@ilifediscoveries.com

• His Tag GCL029 Cloning of DNA of < 1 kb for Expression of

Deliverables : Cloning and Sequencing of Up-stream/

For all prices contact : customersupport@ilifediscoveries.com

This service will include :

Ordering Information Ordering Information

Service No. Description Service No. Description

For all prices contact : customersupport@ilifediscoveries.com

Full-length cDNA Cloning Services Other Related Services

Custom Genomic DNA library construction service

Custom cDNA library construction service will

Site Directed Mutagenesis helps in :

• Study of active sites, structure - function relationships, nucleic

Custom Site Directed Mutagenesis service will include :

mutagenesis procedure or subcloning into another vector

For all prices contact : customersupport@ilifediscoveries.com

For all prices contact : customersupport@ilifediscoveries.com

Other Related Services

(for multiplexing;5 plex)

Microsatellite Analysis: For all prices contact : customersupport@ilifediscoveries.com

Efficient SNP detection methods on standard DNA sequencers are