DAFTAR ISI

HALAMAN JUDUL........................................................................................ i

KATA PENGANTAR ..................................................................................... ii

DAFTAR ISI.................................................................................................... iii

..........................................................................................................................

BAB I PENDAHULUAN................................................................................. 1

A. Latar Belakang ..................................................................................... 1

B. Rumusan Masalah ................................................................................ 3
C. Tujuan Penelitian ................................................................................. 3
D. Manfaat Penelitian ............................................................................... 4

BAB II TINJAUAN PUSTAKA ..................................................................... 5

A. Tanaman Stroberi ................................................................................. 5

1. Sistematika Tanaman Stroberi....................................................... 5
2. Nama Lain ..................................................................................... 5
3. Morfologi Tanaman Stroberi ........................................................ 5
4. Manfaat Tanaman Stroberi ........................................................... 7
5. Kandungan Kimia Tanaman Stroberi .......................................... 8
B. Golongan Senyawa Metabolit Sekunder dalam Tanaman .................. 8
1. Steroid / triterpenoid ..................................................................... 8
2. Flavonoid ...................................................................................... 9
3. Saponin ......................................................................................... 9
4. Senyawa Fenolik ........................................................................... 9
5. Tanin ............................................................................................. 9
C. Ekstraksi .............................................................................................. 9
1. Pengertian Ekstraksi ..................................................................... 9
2. Maserasi ........................................................................................ 10
3. Etanol sebagai pelarut ................................................................... 10
D. Radikal Bebas ......................................................................................
1. Secara Endogen ............................................................................. 11
2. Secara Eksogen ............................................................................. 12
E. Antioksidan .......................................................................................... 12
1. Pengertian antioksidan .................................................................. 12
2. Macam macam Antioksidan ......................................................... 12
2.1................................................................ Antioksidan Primer
............................................................................................12

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 2.2............................................................ Antioksidan Sekunder
............................................................................................12
2.3............................................................... Antioksidan Tersier
............................................................................................13
3. Fungsi Antioksidan ....................................................................... 13
4. Flavanoid sebagai Antioksidan ..................................................... 13
5. Rutin sebagai Antioksidan ............................................................ 13
6. Uji Aktivitas Antioksidan ............................................................ 14
6.1........................... Metode DPPH (1,1 difenil-2-pikrilhidrazil)
.............................................................................................14
6.2........... Metode FRAP (Ferric Reducing Antioksidant Power)
.............................................................................................14
6.3.............. Metode TRAP ( Trapping Antioksidant Parameter)
.............................................................................................14
6.4............................................ Pengujian Penangkapan Radikal
(Radical Scavenger Test) ........................................................ 15
6.5.................... Pengujian Aktivitas Antioksidan dengan Sistem
Linoleat-tiosianat .................................................................... 15
F. Landasan Teori .................................................................................... 15
G. Hipotesis .............................................................................................. 16

BAB III METODE PENELITIAN .................................................................. 17

A. Popolasi dan Sampel ............................................................................ 17

B. Variabel Penelitian .............................................................................. 17
1. Identifikasi Variabel Utama .......................................................... 17
2. Klasifikasi Variabel Utama .......................................................... 17
3. Definisi Operasional Variabel Utama ........................................... 17
C. Bahan dan Alat .................................................................................... 18
D. Jalannya Penelitian .............................................................................. 19
1. Determinasi Tanaman .................................................................. 19
2. Persiapan Bahan ............................................................................ 19
3. Pembuatan Serbuk Buah Stroberi ................................................. 19
4. Penetapan Kadar Kelembaban ..................................................... 19
5. Pembuatan Ekstrak Etanolik ......................................................... 19
6. Identifikasi Kandungan Kimia ...................................................... 20
6.1................................................ Identifikasi Fenolik dan tanin
............................................................................................20
6.2............................................................ Identifikasi Flavonoid
............................................................................................20

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 6.3.................................... Identifikasi Triterpenoid atau Steroid
............................................................................................20
6.4............................................................... Identifikasi Saponin
............................................................................................20
7. Pembuatan Larutan DPPH ............................................................ 21
8. Penetapan panjang gelombang Maksimal DPPH ......................... 21
9. Penentuan Operating Time DPPH ................................................ 21
10. Uji Aktivitas Antioksidan ............................................................. 21
E. Analisi Hasil ........................................................................................ 22
F. Skema Jalannya Penelitian .................................................................. 22

KESIMPULAN ......................................................................................... 23

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DAFTAR PUSTAKA ..................................................................................... 23

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