pression Kit
S ilenCircleTM RNAi system (pro-
tected under US patent 7,294,504
and additional patents pending) is Box 1 | Product Summary
a plasmid-based RNA interference
Catalogue Numbers ABP-RI-SC02010
system that uses engineered human
ABP-RI-SC02020
U6 RNApolymerase III promoter and
modified terminator for high level Components pre-cut pSIlenCircle Vector (10ng/µl)
small hairpin RNA (shRNA) or siRNA pSilenCircle sequencing Primer (20µM)
expression inside mammalian cells. p53-top (coding for p53 RNAi insert top strand)
The design enables precise start and (20µM)
end of an interfering RNA with the p53-bot (coding for p53 RNAi insert bottom
optimal 3’ overhanging nucleotides. strand) (20µM)
The pre-cut linear vector is ready-to- Storage All reagents to be stored at -20°C.
ligate for construction of interfering
RNA expression plasmid with nearly Stability All components are stable for 6 months when
zero self-ligation. Bearing a neomycin stored properly.
resistant marker, it may be used for
establishing siRNA-expressing stable
cell lines. SilenCircle RNAi system
has been successfully used to knock- Reagents Provided with Design of Inserts
down expression of both endogenous the Kit
and exogenous genes.
siRNA may be produced from two
Method Overview