PERBEDAAN TITER SALMONELLA TYPHI-O DAN SALMONELLA

TYPHI H MENGGUNAKAN PLASMA DAN SERUM

Herlina1, Sri Darmawati2, Budi Santosa2

1. Program Studi D IV Analis Kesehatan Fakultas Ilmu Keperawatan dan Kesehatan

Universitas Muhammadiyah Semarang.
2. Laboratorium Patologi Klinik Fakultas Ilmu Keperawatan dan Kesehatan Universitas
Muhammadiyah Semarang.

ABSTRAK

Uji Widal merupakan tes aglutinasi untuk diagnosis serologi penyakit demam tifoid atau
demam enterik. Uji Widal mengukur level aglutinasi antibodi terhadap antigen O
(somatik) dan antigen H (flagellar). Uji biasanya dilakukan pada serum akut, yaitu serum
yang pertama kali diambil saat pertama kali kontak dengan pasien, minimal harus
didapatkan 1 ml darah untuk mendapatkan jumlah serum yang cukup. Bahan pemeriksaan
uji Widal adalah serum, namun karena keterbatasan volume darah, sekaligus menghemat
waktu pemeriksaan, banyak laboratorium menggunakan sampel plasma sebagai alternatif.
Tujuan penelitian untuk mengetahui perbedaan titer Salmonella typhi-O dan typhi-H
menggunakan plasma dan serum. Jenis penelitian analitik pendekatan cross sectional.
Penelitian terhadap 16 sampel penelitian diperiksa menggunakan bahan serum dan
plasma. Hasil penelitian sampel serum diperoleh Salmonella typhi-O negatif,titer 1/80,
titer 1/160, titer 1/320 berturut-turut sebanyak 2,6,6,2 sampel. Salmonella typhi-H titer
1/80, titer 1/160, titer 1/320 berturut-turut sebanyak 1, 1,11,3 sampel. Sampel plasma
diperoleh. Salmonella typhi-H negatif, titer 1/80, titer 1/160, titer 1/320 berturut-turut
sebanyak 1,7,6,2 sampel. Salmonella typhi-H titer 1/80, titer 1/160, titer 1/320 berturut-
turut sebanyak 1, 13, 2 sampel. Hasil uji statistik dengan Wilcoxon dan Chi Square
diperoleh p > 0,05 yang berarti tidak terdapat perbedaan bermakna Salmonella typhi-O
dan typhi-H sampel serum dan plasma.

Kata kunci : Salmonella typhi O, Salmonella typhi H, serum, plasma

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 THE DIFFERENCE OF SALMONELLA TYPHI-O AND SALMONELLA
TYPHI H TITER USING PLASMA AND SERUM
Herlina1, Sri Darmawati2, Budi Santosa2

1. Study Program of D IV Health Analyst Faculty of Nursing and Health University of

Muhammadiyah Semarang.
2. Clinical Pathology Laboratory Faculty of Nursing and Health Sciences University of
Muhammadiyah Semarang.

ABSTRACT

Widal test is agglutination test for serological diagnosis of typhoid fever or enteric fever.
Widal test measures the agglutination level of antibody towards O antigen (somatic) and
H antigen (flagellar). The test is usually performed on acute serum, that is first serum
which taken at the first time contact with patients, at least must be obtained 1 ml of blood
to get enough serum. Widal testing material is serum, but due to the limited blood
volume, while saving the examination time, many laboratories using plasma samples as
an alternative. The research goal is to know the difference of Salmonella typhi-O and
typhi-H titer using plasma and serum. The research type is analytical with cross sectional
approach. The research towards 16 research samples were examined using serum and
plasma materials. The research result was serum samples obtained negative Salmonella
typhi-O, titer 1/80, titer 1/160, titer 1/320 consecutive as much as 2,6,6,2 samples.
Salmonella typhi-H titer 1/80, titer 1/160, titer 1/320 consecutive as much as 1, 1,11,3
samples. Plasma samples were obtained. Negative Salmonella typhi-H, titer 1/80, titer
1/160, titer 1/320 consecutive as much as 1,7,6,2 samples. Salmonella typhi-H titer 1/80,
titer 1/160, titer 1/320 consecutive as much as 1, 13, 2 samples. The statistic test result
with Wilcoxon and was Chi Square obtained p > 0,05 which means there was no
significant difference of Salmonella typhi-O and typhi-H serum and plasma samples.

Keywords: Salmonella typhi O, Salmonella typhi H, serum, plasma

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