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DR SAIFUL’S

NOTES ON
MEDICAL &
ALLIED HEALTH
PROFESSION
EDUCATION:
STATISTICS &
RESEARCH
METHODOLOGY

Dr. Muhamad Saiful Bahri Yusoff


MD, MScMEd
Content

RESEARCH IN MEDICAL EDUCATION ......................................................2


STEPS IN RESEARCH ................................................................................8
SAMPLING METHOD................................................................................ 11
SAMPLE SIZE........................................................................................... 16
OVERVIEW ON MEDICAL STATISTICS ................................................... 20
STUDY DESIGN........................................................................................ 23
DESCRIPTIVE STATITISTICS.................................................................. 30
HYPOTHESIS FORMULATION AND TESTING ......................................... 38
CONFIDENCE INTERVAL......................................................................... 43
EXPLORATORY DATA ANALYSIS (NUMERICAL DATA)........................... 46
UNIVARIATE ANALYSIS OF NUMERICAL DATA...................................... 49
UNIVARIATE ANALYSIS OF CATEGORICAL DATA ................................. 57
CORRELATION & REGRESSION.............................................................. 61
CORRELATION ..................................................................................... 61
SIMPLE LINEAR REGRESSION (SLR) ................................................... 64
CORRELATION ........................................................................................ 72
NONPARAMETRIC STATISTICS .............................................................. 76
NON-PARAMETRIC TESTS ...................................................................... 80
STATISTICAL ANALYSIS: WHICH TO CHOOSE?..................................... 84
WRITING A RESEARCH PROPOSAL ........................................................ 99
VARIABLES............................................................................................ 102
DATA PRESENTATION........................................................................... 106
Z-Score & IT’S USES.............................................................................. 110
t-test ...................................................................................................... 113
SENSITIVITY & SPECIFICITY................................................................ 114

1
RESEARCH IN MEDICAL EDUCATION

1. Research and types of research:


• How do we develop knowledge?
o Intuitive knowledge (based on “I feel or I think”)
o Authoritative knowledge (based on authorized person view)
o Logical knowledge (based on experience explanation which is
reasonable and logical.)
o Empirical knowledge (based on judgement back up by facts and
usually 90% correct)
• What is research?
o Research is a systematized effort to gain new knowledge – (Redman
& Mory)
o Literally research means search again and again repeatedly.
o Research is an organized and systematic way of finding answers to
questions.
• Research comprises:
o Defining and redefining problems
o Formulating hypothesis or suggested solutions
o Collecting, organizing and evaluating data
o Making deduction and reaching conclusions
o Testing the conclusions to determine whether they fit the
formulating hypothesis (Clifford Woody)

• Types of research
o Basic research and applied research
o Quantitative research and qualitative research
• Qualitative research:
o Ethnography, cognitive anthropology, etc
o Synthetic rather than analytic
o Generally hypothesis generating
o Investigative methods are non-intrusive
o Data are more impressionistic.

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o Research in such a situation is a function of researcher’s insights
and impressions.

Educational Research

History Descriptive Correlational Group comparison

Ethnographic Survey

Experimental Quasi-experimental

Ex Post Facto or
Causal-comparative

Thomas K.Crowl

• Descriptive research
o Include quantitative and qualitative researches.
o Methodologies include observations, surveys, self-report and tests.
o May operates on the basis of hypotheses.
o Deals with naturally occurring phenomena.
• Ethnographic research
o Descriptive and qualitative research.
o Report is detailed verbal description.
o Carried out in natural setting.
o Researcher as participant and observer.
• Survey
o Descriptive
o Quantitative study
• Correlational research
o Investigate the relationship between two or more variables.
o Searching the relationship of variables in natural setting.

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• Group comparison research
o Comparing the values of two or more groups of population.
• Experimental research
o Random selection of the individuals forming the groups
ƒ Experimental group
ƒ Control group
• Quasi-experimental research
o A type of group comparison research.
o Groups are randomly selected.
• Ex Post Facto or Causal-comparative study
o Ex Post Facto in latin is “after the fact”.
o Values of independent variable of two groups are preset (al ready
present).

2. Quantitative/Qualitative Research:
• Deductive
o Begin with a theory and collect data to test.
• Inductive:
o Begin with observations and attempt to explain by generalizing.
• Deductive reasoning
o A type of logic in which one goes from a general statement to a
specific instance.
• Inductive reasoning:
o Involves going from a series of specific cases to a general
statement.
o The conclusion in an inductive argument is never guaranteed.
• Confirmatory
o Experimental
o Quasi-experimental
o Correlational (non-experimental)
• Exploratory
o Qualitative

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3. Qualitative research methods for data collection
• Interviews
• Focus groups
• Survey: open ended questions
• Observations: recorded in field notes
• Document analysis
• What is qualitative data?
o Data in the form of words, rather than numbers, based on:
ƒ Asking open ended questions in:
• Interviews
• Group
• Surveys
ƒ Examination of documents
• Observation of situations and actions, recorded in fields
notes
• Uses of qualitative data
o Some social sciences e.g
ƒ Anthropology
ƒ History
ƒ Psychology
ƒ Sociology
ƒ Public health
ƒ Policy analysis
ƒ Health care evaluation

4. Types of quantitative research design:


• The research design which are commonly used can be divided into
following groups:
o Non experimental design
ƒ Post-test design X O1 no control
ƒ Pretest-post test design O1 X O2 no control

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ƒ Static group comparison X O1 O2 no control
o True experimental design
ƒ Pretest-post test control group design
Exp. Group O1 X O2

R.A
(Random Allocation)
Control O3 O4
ƒ Post test control group design
Exp. Group X O1

R.A

Control O2

o Quasi-experimental design
ƒ Time series O1 X O2 X O3 X O4
ƒ No equivalent control group
• Exp group O1 X O2
• No equivalent control group O3 O4
ƒ Separate sample pretest post test design
• R.A – Pretest group O1 X
• R.A – Post test group X O2
5. Purpose of Medical Education Research:
• To improve the functioning of educational programmes by providing
information for:
o Decision making
o Evaluating outcomes
o Supporting advocacy for change
o Contributing to the body of knowledge related to concepts and
methods.

Research is like a plant that grows and grows and grows and grows…

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When it is grown, it throws off seeds of all types (basic, applied and
practical) which in turn sprout and create more research projects…

The process continues with all of the new research ‘plants’ throwing off
seeds, creating additional, related research projects of various types…

Soon there is a body of basic, applied and practical research projects


related to similar topics…
And the process goes on and on…

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STEPS IN RESEARCH

1. Preliminary steps:
• Clarifying the purpose
• Formulating the topic
o State your topic idea as a question
o Identify the main concepts or keywords

2. Finding background information


• Critically analyzing information sources
o Initial appraisal
ƒ Author
ƒ Date of publication
ƒ Edition or revision
ƒ Publisher
ƒ Title of the journal
o Content of analysis
ƒ Intended audience
ƒ Objective reasoning
ƒ Coverage
ƒ Writing style
ƒ Evaluative review

3. Five steps to write topic for better research


• Think about your topic
• Define your main concepts
• Think synonyms
• Think of broader terms
• Think of narrower terms
4. Steps in research
• Planning
o Formulation of the study objectives

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ƒ General objective – what are the purpose of the study
ƒ Specific objective – what are the things you want o find in the
study
o Planning of methods
ƒ Study population
• Selection and definition
• Sampling
• Sample size
ƒ Variables
• Selection
• Definition
• Scales of measurement
ƒ Method of data collection
ƒ Method of recording and processing
• Preparing for data collection
o Construction of research instrument
o Pretesting the instrument
• Collection the data
• Processing the data
• Interpreting the data
• Writing a report

5. To prioritize a problem and selection of a topic for research, it is helpful to


ask yourself a series of questions and then try to answer each of them
• Is the problem a current one? Does the problem exist now?
• How widespread is the problem? Are many areas and many people
affected by the problem?
• Does the problem effect social groups, such as students, teachers and
patients?
• Does the problem relate to broad social, economic, and health issues,
such as unemployment income maldistribution, the status of women,
education and maternal and child health?

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• Who else is concerned about the problem? Are top government
officials concerned? Are medical doctors or other professionals
concerned?
• Are the resources available?
• Are measures available to solve the problem?
Review your answers to these questions, and ranked the problem and
arrange them according to the ranking.

Problem identification
Dissemination of findings
Report writing

Information gathering &


knowledge building

Drawing inference

Research question/
hypothesis formulation
Confirmation or
rejection of hypothesis

Planning research
Data analysis

Data collection Data processing

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SAMPLING METHOD

Sampling Method

Non-Probability Probability
sampling sampling

Purposive Convenient Unrestricted


sampling sampling sampling
(Simple
random
sampling) Restricted
sampling
Judgment Quota
sampling sampling

Systematic Stratified Cluster Area Double


sampling random sampling sampling sampling
sampling

1. Sample and Subject:


• A sample is a subset of the population; it comprises some members
selected from it.
• A subject is a single member of the sample (just like an element is a
single member of the population).

2. Population, Element and Population Frame:


• Population refers to the entire group of people, events, or things of
interest that the researcher wishes to investigate.
• An element is a single member of the population.
• Population frame is a listing of all the elements in the population from
which the sample is drawn.

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3. Sampling:
• Sampling is the process of selecting a sufficient number of elements
from the population, so that a study of the properties or
characteristics of the sample make it possible to generalize such
properties or characteristics to the population.

4. Two major types of sampling design:


• Probability sampling (sample picked at random).
o The elements in the population have equal chance or probability of
being selected as sample subjects.
o Probability sampling designs are used when the representativeness
of the samples is of importance in the interests of wider
generalisability.
• Non-probability sampling (sample not randomly picked).
o The elements do not have a predetermined chance of being selected
as subjects.
o When time or other factors, rather than generalisability, become
critical, non probability sampling design are chosen.

5. Probability Sampling:
• Unrestricted sampling:
o More commonly known as simple random sampling.
o Every element in the population has a known and equal chance of
being selected as a subject.
o Advantage:
ƒ This kind of sampling method has the least bias.
o Disadvantages:
ƒ Cumbersome (difficult) and expensive.
ƒ An entirely updated listing (population frame) of the population
may not always available.
• Restricted (complex) random sampling:
o Offer a viable, and sometimes more efficient alternative to the
unrestricted design.

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o Five most common complex probability sampling methods
ƒ Systematic sampling
• Drawing every nth element in the population starting with a
randomly chosen between 1 and n.
• For example, if we want a sample of 60 students from total
population of 300 students, we could sample every 9th
student (9, 18, 27, …) until 60 students are selected.
• The number must be selected randomly for example we san
take out one dollar ringgit and choose the last digit of money
number.
ƒ Stratified random sampling
• When sub-population vary considerably, it is advantageous
to sample each sub-population (stratum) independently.
• Stratification is the process of grouping members of the
population into relatively homogenous subgroups before
sampling.
• The strata should be mutually exclusive: every element in the
population must be assigned to only one stratum.
• The strata should also collectively exhaustive: no population
element can be excluded.
• The random sampling is applied within each stratum.
ƒ Cluster sampling
• Cluster sampling is used when natural grouping are evident
in the population.
• The total population is divided into groups or clusters.
• Elements within a cluster should be heterogenous as
possible.
• But there should be homogeneity between clusters.
• Each cluster must be mutually exclusive and collectively
exhaustive.
• A random sampling technique is then used on any relevant
clusters to choose which clusters to include in the study.

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ƒ Area sampling
• One version of cluster sampling is area sampling or
geographically clusters sampling.
• Clusters consist of geographical areas.
• A geographically dispersed population can be expensive to
survey.
• Greater economy than simple random sampling can be
achieved by treating several respondents within a local area
as a cluster.
ƒ Double sampling
• A sampling design where initially a sample is used in a study
to collect some preliminary information of interest, and later
a sub-sample of this primary sample is used to examine the
matter in more detail
• It is like reverse pilot study because in double sampling take
all population then proceeds with sampling the interest sub-
sample.

6. Non-probability Sampling:
• The elements in the population do not have any probabilities attached
to their being chosen as sample subjects.
• The findings from the study of the sample cannot be confidently
generalized to the population.
• This method is chosen when generalisability is not critical; focus may
be on obtaining preliminary information in a quick and inexpensive
way.
• 2 broad categories:
o Convenience sampling
ƒ Collection of information from members of the population who
are conveniently available to provide it.
o Purposive sampling

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ƒ The sampling is confined to specific types of people who can
provide the desired information, either because they are the only
ones who have it, or conform to some criteria set by the
researcher.
ƒ 2 type of purposive sampling:
• Judgment sampling
o Involves choice of subject who are most advantageously
placed or in the best position to provide the information
required.
o Judgment sampling may curtail the generalisability of the
findings because we are using a sample of experts who
are conveniently available to us.
o Judgment sampling calls for special efforts to locate and
gain access to the individually who do not have the
requisite information.
• Quota sampling
o This method ensures that certain groups are adequately
represented in the study through the assignment of a
quota.
o The quota fixed for each subgroup is based on the total
numbers of each group in the population.
o Considered as a form of appropriateness stratified
sampling, in which a predetermined proportion of people
are sampled from different groups, but on a convenience
basis.

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SAMPLE SIZE

1. Introduction:
• Questions:
o How large should my sample be?
• Answer:
o It depends…
ƒ …large enough to be an accurate representation of the
population.
ƒ …large enough to achieve statistically significant results.
2. Determining sample size:
• What is sample size that would be required to make reasonably
precise generalizations with confidence?
• A reliable and valid sample should enable us to generalize the findings
from the sample to the population under investigation.
• The sample statistic (statistic finding) should be reliable estimates and
reflect the population parameter (actual finding) as closely as possible
within a narrow margin of error.
• Precision:
o Precision refers to how close our estimate is to the true population
characteristic.
o Normally, the greater the precision required, the larger is the
sample size needed.
• Confidence:
o Confidence denotes how certain we are that our estimate will really
hold true for the population.
o Confidence reflect the level of certainty with which we can state
that our estimates of the population parameters, based on our
sample statistics, hold true.
o Level of confidence can range from 0 to 100%.
o A level of confidence of 95% is conventionally acceptable.
• Sample size is function of…
o Variability (heterogeneity) in the population

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ƒ The more variance we find, the bigger the sample should be
o Precision or accuracy needed
ƒ The more precise or accurate we want, the bigger the sample
size should be
o Confidence level desired
ƒ The higher the confidence level we want, the bigger the sample
size should be
o Type of sampling plan used
ƒ Different sampling approaches will require different sample size
• Trade-off between confidence and precision
o If there is little variability in the population, a small sample size
will be sufficient to obtain a high confidence and precision level.
o The higher the precision, the lower will our confidence level be.
o The higher the confidence level, the lower will our precision level
be. Æ That is why, in both cases, we need bigger sample size to
increase the precision and confidence.
• Roscoe proposes the following rules of thumb for determining sample
size
o Sample size larger than 30 and less than 500 are appropriate for
most research
o Where samples are to be broken into sub samples, a minimum
sample size of 30 for each category is necessary.
o In multivariate research (including regression analyses) the sample
size should be several times (preferably 10 times or more) as large
as the number of variables in the study.
o For simple experimental research with tight experimental controls,
successful research is possible with samples as small as 10 to 20
in size.
3. The term statistically significant (p<.05) is used merely as a way
indicating the chances are at least 95 out of 100 that the findings obtained
from the sample of people who participate in the study are similar to what
the findings would be if one were actually able to carry out the study with
the entire population.

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4. Sample size for single mean

n = (Z /∆) 2 n = sample size


 = population standard
deviation
∆ = precision
Z = Z-score at significance
level

If there is a possibility of response from 80% of sample population the


sample size = n/0.8
Table of values for A and B:

5. Sample size for two means Significant level A

5% 1.98
n = sample size
1% 2.58
n = (A + B) 2 * 22 /∆2  = population standard
Power B
deviation
∆ = expected difference of 80% 0.84
90% 1.28
mean 95% 1.54
A = significance level
(usually 95%, equals 1.96)
6. Sample size for single proportion:
B = power (usually 80%,

n =equals
sample0.84)
size
n = (Z/∆) 2 p (1-p)
∆ = precision
Z = Z-score at significance
level
p = population proportion

7. Sample size for two proportions:


Power is the probability
that the null hypothesis
n = (A + B) 2 * [(p1 (1-p1)) + (p2 (1-p2))] / (p1 – p2)2 will be correctly rejected
i.e. rejected when there is
indeed a real difference or
n = sample size association. It can also be
A = significance level thought of as “100 minus
(usually 95%, equals 1.96) the percentage chance of
missing real effect” –
B = power (usually 80%, therefore the higher the
equals 0.84) power, the lower the
p1 = first proportion chance of missing a real
effect.
p2 = second proportion 18
Some definition:

Sampling error is the difference of statistically finding between actual


parameter of population

Standard error is means of deviation values between two or more groups of


sample or population.

Standard deviation is means of deviation values between two or more units


of samples or population.

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OVERVIEW ON MEDICAL STATISTICS

1. Some introduction:
• I’m interested in research…
• I’m forced to do research…
• Whatever the reason may be…

2. What should I do?


• How should I start???

3. Let’s make it easily understandable


• Research methods/ approaches – leading the way/ direction
• Statistical applications – tools/ vehicles

4. What do I know? Be honest!!


• Do I know about research methods?
o If know back to basic… go back and read research methods/
approaches
• Do I know about statistical and software application?
• Do I know how to interpret?
o OK… I understand methods and approaches
• So… how to proceed?
• Please try to learn medical statistics
• OK… I agree to learn medical statistics
• Tell me how should I go for it (the easiest way)
• Don’t make it complicated (statistician make statistics more difficult)
• Tell me only statistics for non-statisticians

5. Application of statistics in medical research


• Why use statistics?
o Art statistics differences in medical context due to real effects or
random variation or both

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• Modern viewpoint of statistics
o Aid for making scientific decision in the face of uncertainty
o A valuable tool in decision making whenever one is uncertain about
the state of nature
• Statistics in medicine
o Increasingly prevalent in medical practice
ƒ Hospital utility statistics, auditing, vaccination uptake,
incidence/prevalence of AIDS and so on…
• Statistics is about common sense and good design
• Statistics is only the guide to make decisions
• Judgment should be made based on both biological and statistical
plausibility
• Concept and applications of statistics in medical sciences
o Let us discuss briefly
o People say “stat is boring”
o Let us make it interesting

6. Classification of statistics
• It consist of two parts
o Descriptive statistics
ƒ Concerned with collection, organization, enumeration of the
frequency of characteristics, summarization and presentation of
data.
o Inferential statistics
ƒ Statistical inference
ƒ Analytical in nature
ƒ Consists of a collection of principles or theorems
ƒ Allows researcher to generalize characteristics of a “population”
from the observed characteristics of a “sample”
• Statistical jargons
o Population parameter
ƒ A fixed numerical value which describes a particular
characteristic of a population

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ƒ E.g. 1 – the mean value in the population of a particular
characteristic of interest (mean systolic blood pressure of
Australia adults)
ƒ E.g. 2 – the proportion of individuals in the population with a
particular characteristics of interest (the proportion of low birth
weight babies born in Indonesia)
o Sample statistics
ƒ Varies in value from sample to sample
ƒ Other terms – statistics, summary statistics, point estimate,
effect size, point estimate of the effect size
o The relationship between sample statistics and population
parameters is the basis of statistical inference.

7. Statistical inference
• 2 broad categories
o Hypothesis formulation and testing
o Estimation
ƒ Point estimation
ƒ Interval estimation
(Confidence interval)

8. Concepts of populations, samples and statistical inference


• Statistical analysis of medical studies is based on the key idea that we
make observations on sample of subjects and then draw inferences
about the populations of all such subjects from which the sample is
drawn.
• If the study sample is not representative of the population we may well
be misled and statistical procedures cannot help
• But even a well designed study can give only an idea of the answer
sought because of random variation in the sample
• Thus result from a single sample is subject to statistical uncertainty,
which is strongly related to the size of the sample. (Gardner MJ and
Altman DG, 1988)

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STUDY DESIGN

1. How do we begin to answer the question?


• Start with the building blocks of any design
o Participants of investigation
o Outcomes of investigation
o Direction of inquiry (prospective or retrospective)
o Other considerations (e.g. possibility and resources)

2. Think about our research question?


• Identify the participants of interest
• What are the outcomes of interest

3. About the investigation


• Presence of a comparison group
o Dependent on the objective of the study
o Generally increases the validity of an observed association
• Exposure (risk) (or intervention) and outcome
o Must be measured with as little error as possible

4. Overview of epidemiologic studies:


Design strategies

Descriptive Analytical

Observational Intervention studies


Population Individual studies (experimental; RCT)
(prevalence, (case report,
correlational case series,
studies) comparative Cross-sectional Cohort Case-control
studies with studies studies studies
historical
controls)

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5. Case report
• Strength
o Hypothesis (question) generation
o Clinical observation
• Weaknesses
o May be one off
o Nothing to compare

6. Case series
• Strengths
o Strengthens the hypothesis
o Able to establish temporal relationship
• Weakness
o Nothing to compare

7. Comparative studies with historical controls


• Strength
o Like two case series
o Have something to compare to
• Weaknesses
o May be other differences between groups
o Relies on recoding information being accurate

8. Randomized Controlled Trial


• ‘Goal standard’ test of treatment
• Selection of groups entirely random
• Control group identical to treatment group at start except for
intervention
• Participants/investigators commonly ‘blind’ to group allocation to
reduce bias
• May evaluate good and bad outcomes

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• End point blinding Æ e.g. the pathologist are not given any
information about the study sample slide so the pathologist didn’t
know whose slide it is and he/she will decide based on his/her
independent interpretation about the slide.
• There are few of RCT
o Single blind
o Double blind
o Triple blind
o Multiple blind
o End point blinding
• There are 2 design of RCT
o Parallel RCT
o Cross-over RCT

Population
Population

Eligible subjects
Eligible subjects

Randomization Randomization

Pre-treatment Pre-treatment Test Control


assessment assessment

Outcome assessment
Test Control

Control Test

Post-treatment Post-treatment
assessment assessment
Outcome assessment

Parallel RCT Cross-over RCT


9. Prospective cohort:
• A group of people (cohort) is assembled, none of whom has
experienced the outcome interest

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• On entry, people are classified according to characteristics that might
be related to outcome
• Other names: longitudinal, prospective, incidence studies.

Disease

Exposed
No Disease
Direction of inquiry

Disease

Unexposed
No Disease

• Advantages of cohort studies


o The only way of establishing incidence directly
o Follow the same logic as clinical question: if person exposed, do
they get the disease?
o Exposure can be elicited without the bias
o Can assess if the relationship between exposure and many
diseases
o Calculate risk directly: relative risk (RR)
• Strengths
o Powerful design for defining incidence and investigating potential
causes (aetiology questions)
o Establishes temporal sequences
o Appropriate for interventions where can’t randomize
o Investigator has opportunity to measure important variables
completely (not relying on record information)
• Weaknesses
o Expensive and inefficient for rare outcomes – needs more patients
o May be other differences between group

10. Case control studies


• Analytic study design

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o Looking back in nature
o We were not there to measure risk directly
o Associate outcome (disease) with prior exposure
• Calculate indirect estimate of risk: odds ratio (OR)
• Compare the frequency of a risk factor in a group of cases and a
group of controls
• There must be a comparison group that does not have the disease
• There must be enough people in the study so that chance does not
play a large part in the observed results
• Groups must be comparable except for the factor of interest

Exposed

Cases
Unexposed
Direction of inquiry

Exposed

Control
Unexposed

• Advantages of case-control studies


o No need to wait for a long time for disease to occur (causal or
prognostic factors)
o Most important methods used to study rare disease
o Best design for disease with long latent period
o Can evaluate multiple possible potential exposure
• Strengths
o Very efficient design for rare outcomes
• Weaknesses
o Does not allow for the examination of incidence or risk
ƒ Cannot directly calculate incidence: OR; and indirect estimate
of risk.
o Increased susceptible to bias in measurement of exposure
ƒ Exposure & disease occurred “prior to” the study

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• More potential for biases

11. Cross-sectional study


• Distinguish features
o Observe at on particular time or over a period
o Exposure and outcomes measured at the same time
o Information obtained from the subjects only once

Observation

Population

Samples

Exposed Disease

Unexposed No Disease

• Categories of cross-sectional study


o Descriptive
ƒ Prevalence studies
• A point prevalence
o Disease occurrence at the particular time
o E.g. the point prevalence of upper respiratory tract
infection on 1st of July 2005
• A period prevalence
o Disease occurrence at the particular period of time
o E.g. the ten-year year period prevalence (1996-2005) of
the cancer of breast in Malaysia.
o Analytical
ƒ Analytical studies is valid only when the current values of the
exposure are extremely stable over time
ƒ Two types

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• Classical cross-sectional
• Comparative cross-sectional study
o A comparative way of conducting a cross-sectional study
o Samples are drawn from two or more defined different
populations
o Measure exposure and outcome factors
o Investigate the association between exposure and
outcome
o Strengths of cross sectional studies
ƒ Very quick and inexpensive to implement
ƒ Useful for determining prevalence
ƒ Appropriate for diagnostic test validity
o Weaknesses
ƒ Difficulty in establishing links of causal effect (temporal
relationship)
ƒ Impractical for rare outcomes

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DESCRIPTIVE STATITISTICS

1. Definition:
• Statistics
o A field of study concerned with the collection, organization and
summarization of data.
• Statistical Methods
o A scientific technique employed for collection, presentation,
analysis and interpretation of data.
• Biostatistics
o Biological field and medicine
2. Uses of statistical methods
• To collect data in the best possible way
o Designing form
o Organizing
o Conducting survey
• To describe the characteristics of a group or a situation
o Data summary
o Data presentation
• To analyses data and to withdraw conclusion
o Scientific, logic
o Decision making
3. Classification of statistics
• Descriptive statistics
o Concerned with collection, organization, enumeration of frequency
of characteristics, summarization and presentation of data.
o Describe characteristics of the observed data
ƒ Type of variable
ƒ Summary statistics
ƒ Distribution
ƒ Graphical presentation
• Inferential statistics
o Analytical in nature

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o Involve hypothesis testing and confidence interval
o Allows researcher to infer/ generalize the characteristics of the
sample (statistic) to the population (parameter)
4. Terms:
• Population:
o Full sets of individuals
o Collection of items objects, things, people
o Parameter – descriptive measure from population data
• Sample
o Subset of population
o Selected to represent the population by sampling technique
o Statistics – descriptive measure from sample data
• Variable
o Any characteristics of even/object/person
o The characteristics being observed/measured
o E.g. age sex, race, height, weight, etc
• Data
o The raw or original measurement of statistics
o Values taken by the characteristics
o E.g. Malay, female, 155cm
5. Classification of variables

31
• Discrete
o Characteristics by gaps or interruptions in the values
o Values that can be assume only whole numbers
o Mainly count
o E.g. no of students, no of teeth extracted
• Continuous
o No gap or interruption
o Any value within specified interval
o Mainly measurement
o E.g. height, weight, BP, age, etc
• Nominal
o Unordered categories
o No implied order among the categories
o E.g. race, sex, medical diagnosis, etc.
• Ordinal
o Ordered categories
o Ranked according to some criteria
o E.g. BP – high, normal, low.

32
6. Categorical Variables:
• Data presentation
o Statistics
ƒ Frequency
ƒ Percentage (%)
o Graphical
ƒ Pie chart
ƒ Bar chart

7. Numerical variables
• Measures of central tendency
o A measure of centrality

ƒ Mean
• Arithmetic average
• Adding all the values in a population/sample and divided by
the number of values that are added
• Affected by the extreme value

33
ƒ Median
• The middle value of data ordered from the lowest to the
highest Æ arrange all value in order
• If n = odd number Æ medical is the middle
• If n = even number Æ median is the mean of 2 middle
observation
• 50th percentile of a set of observation
• The middle value of data ordered from lowest to the highest
value
• Useful for data with non-normal distribution or skewed data
• Less sensitive to extreme values than the mean
• Median (IQR)
ƒ Mode
• The most frequent observation
• Point of maximum concentration
• Measures of dispersion/variability
o Range = largest value – smallest value
ƒ Different between the largest and smallest value in a set of
observations
ƒ Give idea about the variability of data
ƒ Simplest to compute
ƒ Sensitive to outliers
ƒ Least useful
ƒ R = Xmax - Xmin
o Variance = s2
ƒ Total squares of deviation of observations from the
mean/number of degree of freedom
ƒ Average measure of standard deviation of observation from
mean sample
ƒ Measures the amount of variability or spread about/from the
mean of a sample
ƒ S2 = Σ(xi – xmean)2/n-1

34
o Standard deviation (SD)
ƒ A square root of variance
ƒ The root mean square of the distances (or differences) from
mean of sample
ƒ A better measure of variability of a set of data
ƒ Smaller SD indicates closer to the mean
ƒ Mean (SD)
ƒ S = √[Σ(xi – xmean)2/n-1]

o Interquartile range (IQR)


ƒ Q3 – Q1
ƒ Range between 25th and 75th percentile
ƒ Used along with the median
ƒ It not affected by outlier
o Percentile = 25th, 50th, 75th, 90th, 95th
8. Normal distribution
• Characteristic
o Bell shaped appearance
o Symmetrical about the mean
o Mean = median = mode
o Total area the curve = 1
o The curve never touch the x line
o SD usually less than 30% of mean value
• Approximately
o 68% Æ 1 SD

35
o 95% Æ 2 SD
o 99.7% Æ 3SD
• Mean (SD)

9. Data presentation (numerical data)


• Statistics
o Mean (SD)
o Medical (IQR)
• Graphical
o Histogram
ƒ Frequency distribution of quantitative date/continuous data
ƒ Bars represent frequency distribution for each class of interval
ƒ No spaces between bars
ƒ May have equal/unequal class interval
o Box plot

36
Histogram

10. Summary
• Categorical data
o Statistics
ƒ Frequency (%)
o Graphs
ƒ Bar chart
ƒ Pie chart
• Numerical data
o Statistics
ƒ Mean (SD)
ƒ Median (IQR)
o Graphs
ƒ Histogram
ƒ Box Plot

37
HYPOTHESIS FORMULATION AND TESTING

1. Hypothesis:
• A statement about one or more population
• Research question
o Statement
o Research hypothesis
• Postulating the existence of:
o A difference between groups
o An association among factors
• Usually derived from a hunch, an educated guess based on published
results or preliminary observations.
• There are 2 type:
o Null hypothesis (HO)
ƒ Hypothesis of no difference
ƒ Hypothesis to tested
o Alternative hypothesis (HA)
ƒ The hypothesis that postulates that there is a treatment effect,
an association factors or a difference between groups.
• Inferential statistics – estimating the probability that a given outcome
is due to chance
• If the sample data provide sufficient evidence to discredit HO Æ reject
HO in favor of HA.

2. Hypothesis Testing:
• To aid the researcher in reading a decision concerning a population y
examining the sample.
• Observed differences or associations may have occurred by chance.
• HO : the proportion of patients with disease who die after treatment
with the new drug is not different from the proportion of similar
patients who die after treatment with placebo.

38
• HA : the proportion of patients with disease who die after treatment
with the new drug is lower than the proportion of similar patients who
die after treatment with placebo.

Statistical Decision In the population


based on a sample HO true HO false
Correct Type II error
(Confidence Limit) Beta (β)
Do not reject HO 1– (probability of wrongly not
(level of certainty of our rejecting HO when the HO
statistical data hold true) is false)
Type I error
Correct
(level of significance)
(Power of study)
Alpha ()
Reject HO 1–β
(probability of wrongly
(probability that the HO
rejecting the HO when the
correctly rejected)
HO is true)

• Type I error ()


o The probability of wrongly rejecting the null hypothesis when the
null hypothesis is true.
• Type II error (β)
o The probability wrongly not rejecting the null hypothesis when
the null hypothesis is false.
• The test statistics
o A value with a known distribution when the null hypothesis is true.
• Normal distribution (refer to descriptive statistics note)
• Level of significant ()
o The null hypothesis is rejected if the probability of obtaining a
value as extreme or more extreme than that observed in the sample
is small when the null hypothesis is true.
o “Small” is usually taken to less than or equal to 5%
o If the 2 tail test is taken then the  must be divided by 2

39
ƒ E.g.  = 0.05, p value = 0.05, (2 tail test taken). Significant level
 = 0.05/2 = 0.25. Conclusion does not reject the HO.
• The p value
o The probability of obtaining a value as extreme or more extreme
than that observed in the sample given that the null hypothesis is
true is called p value
o The smallest value of  for which the null hypothesis can be
rejected
o The p value is compared to the predetermined significance level 
(usually 0.05) to decide whether the null hypothesis should be
rejected
o If p value less than , reject the HO.
o If p value greater than , do not reject the HO.

3. Steps in hypothesis testing


• Step 1
o Generate the null hypothesis and alternative hypothesis
ƒ HO : ??
ƒ HA : ??
ƒ What are the characteristics of interest?
• E.g. mean, proportion
• 1-tail (one sided) or 2-tail (both sided)
o E.g. 1-tail research hypothesis
ƒ The proportion of patients with disease after treatment
with new drug is lower than the proportion of similar
patients who die after treatment with placebo
o E.g. 2-tail research hypothesis
ƒ The mean blood pressure of patients in the new
treatment group is not different from the mean blood
pressure of patients in the old treatment
ƒ E.g. research questions:
• Effectiveness of a new antihypertensive drug

40
• HO: the mean blood pressure of patients in the new
treatment group is not different from the mean blood
pressure of patients in the old treatment (µ1 = µ2)
• HA: the mean blood pressure of patients in the new treatment
group is different from the mean blood pressure of patients
in the old treatment (µ1 ≠ µ2)

Notes: Notes:

1. in population 2. in sample

- µ = mean - x = mean
-  = standard deviation - SD = standard deviation

• Step 2:
o Set the significance level
ƒ Usually set at 0.05, 0.01, 0.1
• Step 3:
o Decide which statistical test to use and check the assumption of the
test
ƒ Population is approximately normally distributed
ƒ Data values are obtained by independent random sampling
ƒ Adequate sample size
o To decide which statistical test should be used
ƒ E.g. mean, proportion
o Assumption must be adequately met
o If not met alternative procedures can be used
ƒ E.g. non parametric test would be used when the data is seriously
non-normal)
• Step 4:
o Compute the test statistic and associated p value
ƒ Calculate appropriate test statistics
• Step 5:
o Interpretation
ƒ Compare p value with the level of significance
ƒ Decide whether or not to reject the null hypothesis
ƒ p value <  – reject the null hypothesis

41
ƒ p value >  – do not reject the null hypothesis
• Step 6:
o Draw conclusions
ƒ Conclude accordingly based on rejecting/not rejecting null
hypothesis
o Decision rule
ƒ Rejection region
• To reject the null hypothesis if the value of the test statistic that
computed from the sample is one of the values in the rejection
region
ƒ Acceptance region
• To accept the null hypothesis of the computed values in the
acceptance region
ƒ E.g. conclusion:
• The mean blood pressure of patients in new treatment group is
different from the mean blood pressure of patient in old
treatment

42
CONFIDENCE INTERVAL

1. Relationship between confidence interval and hypothesis test

0.95

Confidence
interval

2. Confidence Interval
• Standard Deviation (SD) vs. Standard Error (SE)

Sample Population
Mean (µ)
SD ()

Mean (x)
SD (s) Sample
Sample

Standard
Error

o SD – a measure of the variability of individual observation


o SE – a measure of variability of summary statistics
ƒ E.g. variability of the sample mean or sample proportion
o SE (SEM) – a special type of standard deviation (the standard
deviation of a sample statistics), depend on
ƒ Standard deviation
ƒ Sample size

43
o Sample mean varies from sample to sample (as measured by SE)

How Close?

Sample Population
Mean (µ)
SD ()

o Sample to sample variation of the statistic (sample statistic)

Lower limit Confidence Upper limit


Interval

Likely to
fall

Population
parameter

44
3. General Comments on Confidence Interval
• As a measure of an estimate of a population parameter (a measure of
the precision of a sample statistic)
• Confidence interval = estimate ± k x (standard error)
• 90% CI, 95% CI, 99% CI
o 95% CI interpretation – 95% certain that the population parameter
lies within its limits.

Width of the CI depend on

SE

Sample size More variation


(larger Æ CI narrower CI wider
more precise estimate)

Less precise estimate

• Confidence Interval can be calculated:


o Mean
o Relative risk
o Odds ratio
o Hazards ratio
o Correlation coefficient
o Regression coefficient
o Etc…

45
EXPLORATORY DATA ANALYSIS (NUMERICAL DATA)

1. Hypothesis test for:


• Single mean
• Difference between two means for independent samples
• Difference between two means (or paired) samples.

2. Single Mean:
• Step 1: Null and Alternative Hypothesis
o H0: The mean serum amylase level in the population from which
the sample was drawn is 120 units/100ml.
o HA: The mean serum amylase level in the population from which
the sample was drawn is different from 120 units/ 100ml. (two-
sided test)
• Step 2: Level of significance
o Alpha = 0.05 (alpha/2 = 0.025)
• Step 3: Check the assumption
o Population is approximately normally distributed
o Random sampling
o Independent variable/sample
• Step 4: Statistical test (one sample t test)
o t = (x - µ0)/ (s/√n)
o where
ƒ x = sample mean
ƒ s = sample standard deviation
ƒ n = sample size
ƒ µ0 = the hypothesis mean
ƒ t stat has n-1 degrees of freedom
• step 5: interpretation
o p-value < 0.025
o reject H0
• step 6: conclusion

46
o The population mean serum amylase is statistically significantly
different from 120 units/ 100ml.

3. Difference between two means for independent samples


• Step 1: Null and Alternative Hypothesis
o H0: The mean serum amylase level in hospitalized and healthy
subjects are the same (µ1 = µ2)
o HA: The mean serum amylase levels in hospitalized and healthy
subjects are different (µ1 ≠ µ2) Æ (two-tailed)
• Step 2: Level of significance
o Alpha = 0.05 (alpha/2 = 0.025)
• Step 3: Check the assumption
o Two population are normally distributed
o Two population have equal variance (Levene’s test)
o Both are independent samples/variables
o Random samples
• Step 4: Statistical test
o Name of the test = independent t-test
o t statistic = (x1 – x2)/√[s2p (1/n1 + 1/n2)]
ƒ where: s2p = [ (n1-1)s21 + (n2-1)s22 ]/[n1 + n2 – 2]
• x1, x2 = sample means
• s1, s2 = sample standard deviations
• n1, n2 = sample size
• t stat has n1 + n2 – 2 degree of freedom (df)
o Degree of freedom
o p-value
o 95% confident interval (lower border & upper border)
• Step 5: Interpretation
o P-value < 0.025
o Reject null hypothesis
• Step 6: Conclusion

47
o At the 5% level of significance that the mean serum amylase levels
are different in healthy and hospitalized subjects.

4. Difference between two means for dependent (or paired) samples


o Research question: the investigators wanted to determine if treatment
with Amynophylline altered the average number of apneic episodes per
hour
o Step 1: Null and Alternative Hypothesis
o H0: there is no difference in average number of apneic episodes
before and after Amynophylline (no difference = zero)
o HA: the average number of apneic episodes before and after was
difference (difference not equal to zero) (two-tailed)
o Step 2: Level of significance
o Alpha = 0.05 (alpha/2 = 0.025)
o Step 3: Check the assumption
o The population are normally distributes
o The two samples are dependent variables/samples
o Random sampling
o Step 4: Statistical test (paired t test)
o t = d/sd √(n)
o where
ƒ d = means of differences
ƒ sd = standard deviation of the differences
ƒ n = number of pairs
ƒ t stat has n-1 degree of freedom
o Step 5: Interpretation
o p-value < 0.025
o reject null hypothesis
o Step 6: Conclusion
o At the 5% level of significance that the average number of apneic
episodes before and after Amyn0phylline were difference.

48
UNIVARIATE ANALYSIS OF NUMERICAL DATA

1. Univariate analysis explores each variable in a data set separately:


• It looks at the range of values
• The central tendency of the values
• It describes the pattern of responsible to the variable
• It describes each variable on its own
2. Univariate analysis
• Categorical variable (e.g. housing)
• Numerical variable (e.g. age)
3. Univariate analysis - Numeric
• Statistical analysis
o Point estimation
ƒ Count, min, max, average, median, mode.
o Dispersion
ƒ Range, standard deviation, variance, co-variance
ƒ Skewness, kurtosis
o Missing value
o Outliers
o Binning
• Visualization
o Histogram, box plot and etc…

Univariate Analysis – Numeric

Age
Count 900 Average 35.25 St Dev 11.20
Min 19 Median 33 Variance 125.37
Max 75 Mode 27 Covariance 32%
Range 55 Skewness 1.09
Missing 0 Kurtosis 0.88

49
Univariate Analysis – Challenges

Variable
Categorical Numeric
Missing values Missing values
Invalid values Outliers
Numerization Binning

4. Missing data
• Data entry error
• Data processing error
• Certain data may net be available at the time of entry
• How to handle missing data
o Fill in the missing values manually
o Ignore the records with missing data
o Fill in it automatically
ƒ A global constant (e.g. “?”)
ƒ The variable mean
5. Outliers
• Data points inconsistent with the majority of data
• Different outliers
o Valid: CEO’s salary
o Noisy: one’s age = 200, widely deviated points
• Removal methods
o Box plot
o Clustering
o Curve-fitting
6. Binning
• Binning is a process of transferring continuous variables into
categorical counterparts
• Binning methods
o Equal-width

50
o Equal-frequency
o Entropy-based methods
• Variable values (e.g. age)
o 0, 4, 12, 16, 16, 18, 24, 26, 28
• Equal-width binning
o Bin 1: 0, 4 [-, 10] bin
o Bin 2: 12, 16, 16, 18 [10, 20] bin
o Bin 3: 24, 26, 28 [20, +] bin
• Equal-frequency
o Bin 1: 0, 4, 12 [-, 14] bin
o Bin 2: 16, 16, 18 [14, 21] bin
o Bin 3: 24, 26, 28 [21, +] bin
7. Numerization
• Numerization is the process of transferring categorical variable into
numerical counterparts.
• Numerization methods
o Binary method
o Ordinal method
• Variable values (e.g. housing
o For free, own, rent
• Binary method
o For free: 1, 0, 0
o Own: 0, 1, 0
o Rent: 0, 0, 1
• Ordinal method
o Own: 5
o For free: 3
o Rent: 1
8. Quantification
• Introduction
o To conduct quantitative analysis, responses to open-ended questions
in survey research and the raw data collected using qualitative
methods must be coded numerically.

51
o Most responses to survey research questions already are recorded in
numerical format
ƒ In mailed and face-to-face surveys, responses are keypunched
into a data file.
ƒ In telephone and internet surveys, responses are automatically
recorded in numerically format.
• Developing code categories
o Coding qualitative data can use an existing scheme or one developed
by examining the data.
o Coding qualitative data into numerical categories sometimes can be
a straightforward process
ƒ Coding occupation, for example, can rely upon numerical
categories defined by the Bureau of the census.
o Coding most forms of qualitative data, however, requires much effort
o This coding typically requires using an iterative procedure of trial
and error
o Consider, for example, coding responses to the question, “What is
the biggest problem in attending college today?”
o The researcher must develop a set of codes that are;
ƒ Exhaustive of the full range of responses
ƒ Mutually exclusive (mostly) of one another.
o In coding responses to the question, “What is the biggest problem in
attending college today?” the researcher might begin, for example,
with a list of 5 categories, then realize that 8 would be better, then
realize that it would be better to combine and use a total of 7
categories
o Each time the researcher makes a change in the coding scheme, it is
necessary to restart the coding process to code all responses using
the same scheme
9. Distribution
• Data analysis begins by examining distributions

52
• One might begin, for example, by examining the distribution of
responses to a question about formal education, where responses are
recorded within six categories
• A frequency distribution will show the number and percent of
responses in each category of a variable
10. Central tendency
• A common measure of central tendency is the average or mean of the
responses
• The median is the values in the middle case when all responses are
rank-ordered
• The mode is the most common responses
• When data are highly skewed, meaning heavily balanced toward one
end of the distribution, the median or mode might be better represent
the most common or centered response.
• Consider this distribution of respondent ages:
o 18, 19, 19, 19, 20, 20, 21, 22, 85
• The mean equals 27. But this number does not adequately represent
the common respondent because the one person who is 85 skews the
distribution toward the high end.
• The median equals 20
• This measure of central tendency gives a more accurate portrayal of the
middle of the distribution
11. Dispersion
• Dispersion refers to the way the values are distributed around some
central value, typically the mean.
• The range is the distance separating the lowest and highest values (e.g.
the range of the ages listed previously equals 18-85)
• The standard deviation is an index of the amount of variability in a set
of data
• The standard deviation represent dispersion with respect to the normal
(bell shape) curve

53
• Assuming a set of numbers is normally distributed, then each standard
deviation equals a certain distance from the mean.
• Each standard deviation (+1, +2, etc) is the same distance from each
other on the bell-shaped curve, but represents a declining percentage of
responses because of the shape of the curve.
• For example, the first standard deviation account 34.1% of the values
below and above the mean
o The figure 34.1% is derived from probability theory and the shape of
the curve.
• Thus approximately 68% of all responses fall within one standard
deviation of the mean
• The second standard deviation accounts for the next 13.6% of the
responses from the mean (27.2% of all responses) and so on.
• Dispersion measures
o Spread around the mean
ƒ Variance – too abstract, a step towards standard deviation
ƒ Standard deviation (from mean) – more intuitive
o Standard deviation
ƒ Average distance between mean and each value in data set
ƒ Translates variance into same scale as mean and all the values
ƒ High values are generally bad
• If the responses are distributed approximately normal and the range of
responses is low – meaning that most responses fall closely to the mean
– then the standard deviation will be small
o The standard deviation of professional golfer’s score on a gold course
will be low
o The standard deviation of amateur golfer’s scores on a golf course
will be high
13. Continuous and Discrete Variables
• Continuous variables have responses that form a steady progression
(e.g. age, income)
• Discrete (i.e. categorical) variables have responses that are considered
to be separate from one another (i.e. sex, religious)

54
• Sometimes, it is matter of debate within the community of scholars
about whether a measured variable is continuous or discrete
• This issue is important because the statistical procedures appropriate
for continuous-level data, especially as related to the measurement of
the dependent variable.
• Example: suppose one measures amount of formal education within
five categories (less than hs, hs, 2 years vocational/college, college, post
college)
• Is this measure continuous or discrete?
• In practice, five categories seem to be cut off point for considering a
variable as continuous
• Using a seven-point response scale will give the researcher greater
chance of deeming a variable to be continuous.
14. Subgroup comparison
• Collapsing response categories
o Sometimes the researcher might want to analyse a variable by using
fewer response categories than were used to measure it
o In these instances, the researcher might want to collapse one or
more categories into a single category
o The researcher might want to collapse categories to simplify the
presentation of the results or because few observations exist within
some categories
• Collapsing response example

Response Frequency
Strongly disagree 2
Disagree 22
Neither agree nor disagree 45
Agree 31
Strongly agree 1

55
One might want to collapse the extreme responses and work with just three
categories

Response Frequency
Disagree 24
Neither agree nor disagree 45
Agree 32

• Handling “Don’t Know”


o When asking about knowledge of factual information (“Does you
teenager drink alcohol?”) or opinion on a topic the subject might not
know much about (“Do school officials do enough to discourage
teenagers from drinking alcohol?”), it is wise to include a “don’t
know” categories as a possible responses.
o Analyzing “don’t know” responses, however can be a difficult task
o The research-on-research literature regarding this issues is complex
and without clear-cut guidelines for decision making
o The decisions about whether to use “don’t know” response categories
and how to code and analyse them tends to be idiosyncratic to the
research and the researcher.

56
UNIVARIATE ANALYSIS OF CATEGORICAL DATA

1. Categorical data analysis


• One proportion
o Chi-square goodness of fit
• Two proportion (independent sample)
o Pearson chi-square/ fisher Exact test
• Dependent sample (matched or paired)
o Mc Nemar’s test
• Stratified sampling to control cofounder effect
o Mantel-Haenszel test
2. Two proportion (independent sample) – Pearson Chi-square & Fisher
Exact test.
• To test the association between two categorical variable
• IHD vs. Gender
o Does gender associated with IHD status?
• Result of test
o Not significant Æ no association
o Significant Æ an association
• Step 1: State the hypothesis
o H0: There is no association between gender and IHD
o HA: There is an association between gender and IHD
• Step 2: set the significance level
o How much? – accept the error in estimating the proportion in the
population
o Usually:  = 0.05
• Step 3: check the assumption
o Two variables are independent
o Two variables are categorical
o Expected count of less than 5 is > 20% (take fisher exact test) and
if < 20% (take pearson chi-square test).
ƒ Expected count = [row total x column total]/grand total

57
• Step 4: statistical test
o Chi-square test or
o Fisher exact test
o X2 = Σ (O – E)2 / E
o Chi-square value:
ƒ When the difference between observed and expected increase Æ
Value of chi-square increase Æ p-value decrease Æ significant
increase
• Step 5: Interpretation
o p value = 0.123
ƒ do not reject H0
o There is no significant association between gender and IHD status.
• Step 6: conclusion
o There is no significance association between gender and IHD status
using Pearson Chi-square tests (p-value = 0.123)
• Data presentation

Table 1: Association between IHD and gender


IHD
Variable Yes No z stat p-value
n (%) n (%)
Gender
Male 15 (60) 10 (40) 2.381 *0.123
Female 20 (80) 5 (20)
* Pearson Chi-square test
3. Two proportion (dependent sample) – Mc Nemar’s test
• Dependent sample (matched or pair sample)
• X2 = (|b+c|) / (b+c) SM
• Discordant pair Live Die
o Is pair of different outcome
Live a** *b
o Use to test the difference in the outcome
• Sample of 25 pair patient with breast cancer RM
Die *c d**

* Discordant
** Concordant 58
o Matched for age
o Undergone
ƒ Simple Mastectomy (SM)
ƒ Radical Mastectomy (RM)
o Difference of 5-year survival proportion between two group
• Step 1: state the null and alternative hypothesis
o H0: there is no association between type between type of
mastectomy and 5-year survival proportion in patients with breast
cancer
o HA: there is an association between type of mastectomy and 5-year
survival proportion in patients with breast cancer.
• Step 2: set the significance level
o  = 0.05
• Step 3: check the assumption
o Categorical data
o Dependent or matched sample
• Step 4: statistical test
o Mc Nemar’s test
• Step 5: interpretation
o p-value = 0.021
ƒ reject H0
o there is significant association between type of mastectomy and 5-
year survival proportion in patients with breast cancer.
• Step 6: conclusion
o There is significant association between type of mastectomy and 5-
year survival proportion in patients with breast cancer using Mc
Nemar’s test (p-value = 0.021)
• Data presentation

59
Table 2: Association between type of mastectomy and 5-year survival
proportion in patients with breast cancer
Simple mastectomy
Variable Live Die p-value
n (%) n (%)
Radical
Live 13 (%) 1 (%) *0.021
Die 9 (%) 2 (%)
* Mc Nemar’s test

60
CORRELATION & REGRESSION

1. Relationship between two variables


• Are two variables associated each other?
• To what degree (strength) are they associated?
• In which directions is the relationship?
o Positive or negative
• Change in dependent variable that corresponds to change in
independent variable.
o Prediction

Correlation Regression

- presence of association - prediction


- strength (degree) of
association
- direction of association

CORRELATION

1. Is a measure of relationship between two numerical variables


- E.g. the relationship between height and weight, the relationship
between cholesterol and blood pressure.
2. Pattern:
- Elliptical pattern – degree of elongation of the ellipses – proportional to
the correlation coefficient.
- Elliptical pattern – indicative of normally distributed variables

61
3. Correlation coefficient (r)
- X increase Y increase r = 1 (perfect positive)
- X increase Y decrease r = -1 (perfect negative)
- No linear relationship r=0
- r
o < 0.25 Æ poor
o 0.26 – 0.50 Æ fair
o 0.51 – 0.75 Æ good
o 0.76 – 1.00 Æ excellent
- r does not imply a cause and effect relationship
- Correlation should be assessed mathematically, not visually.
- r for statistical sample, ρ (rho) for parameter of population.
- Correlation coefficient:

Pearson’s Correlation coefficient Spearman’e Ranked Correlation


coefficient

- A measure of degree of - Correlation coefficient


straight line relationship calculated on the ranks of
between two numerical the observation of two
variable variables
- At least one variable have a - Rank correlation and
normal distribution Spearman’s correlation –
similar
- Different when the scatter
plot deviates from an
elliptical shape

4. Example: Relationship between height and weight


- Step 1: state the null and alternative hypothesis
• H0: There is no correlation between height and weight
• HA: There is correlation between height and weight (2-tailed)
- Step 2: set significance level

62
•  = 0.05
- Step 3: check the assumption
• Both numerical variable
• One of the variables has normal distribution
ƒ Histogram
ƒ Box and Whisker plot
- Step 4: statistical test
• Pearson correlation (if assumption is met)
• Spearman’s correlation (if assumption is not met)
- Step 5: Interpretation
Correlations

weight
height Height Weight
height Height Pearson
1 .878(**)
Correlation
Sig. (2-tailed) . .000
N 100 100
weight Weight Pearson
.878(**) 1
Correlation
Sig. (2-tailed) .000 .
N 100 100
** Correlation is significant at the 0.01 level (2-tailed).

• p-value = <0.001
ƒ reject H0
- step 6: conclusion
• There is a significant, positive and excellence correlation between
height and weight (r = 0.88, p < 0.001)
- Checklist for reporting correlation (Figure 1)
• Correlation coefficient – Pearson’s correlation coefficient/
Spearman’s Ranked correlation coefficient
• Actual p-value of correlation coefficient
• Sample size
• Scatter plot

63
Figure1: A scatter plot showing high positive correlation between
height and weight

80 n = 100, Pearson’s r = 0.88, p < 0.001

75

70
Weight

65

60

55

150 155 160 165 170 175 180


Height

SIMPLE LINEAR REGRESSION (SLR)

1. Regression Analysis
• Regression analysis is a statistical tool that utilizes the relation
between variables so that one variable van be predicted from the other
or others
• Linear regression
o Simple (one independent variable (factor) and one outcome)
o Multiple ( more than one factor and one outcome)
• Logistic Regression (dichotomous dependent variables)

64
2. Simple Linear Regression
• Example of research questions
o Does a relationship exist between oral contraceptive and the
incidence of thromboembolism?
o What is the relationship of a mother’s weight to her baby’s birth
weight?
o Relationship between an animal’s pulse rate and the amount
particular drug administered?
• Simple because only one independent variable
• Linear means the relationship between y (dependent/outcome) and x
(independent/factor) variables can be represented by a straight line
• Analysed linear relationship between two quantitative (numerical)
variables
• Involves estimating the equation of a straight line that defines the
relationship between a dependent variable using a given data set
• The method involved is called method of least squares
• We choose a line such that the sum of squares of vertical distances of
all points from the line is minimized (Q = Σ е2i )
• These vertical distances between y values and their corresponding
estimated values on the line are called residuals (ei = yi – ŷi)
• The line thus obtained is called the regression line or the least-squares
line of best fit
3. Regression line (least squares line of best fit)
• Yi = β0 + β1Xi + єi
o Yi is the value of dependent variable when the value of the
independent variable is Xi
o β0 is Y-interception and is constant
o β1 is the slope of the regression line. It is the change in Yi when Xi is
increased by one unit
o β0 and β1 are called regression coefficients
o єi is random error terms, normally distributed, independent, with
zero mean, and constant variance a2

65
4. Linear Regression Model
• Relationship Independent
(factor/
Population Population explanatory)
Y-interception slope variable

Dependent
(outcome/ Yi = β0 + β1Xi + єi Random error
response) variable

5. True Regression line


• The random error term єi the regression equation accounts for the
scattering of the data points about the regression line
• As the mean of the єis is zero, the mean of Yi (at Xi) is:
o E (Yi) = β0 + β1Xi
o The notation E (Yi) means ‘expected value’ of Yi and represents the
mean of Yi
• Not that the mean of y and on x and the relationship is represented by
a straight line
• This equation represents the true regression line
6. Least square estimate
• Time regression line is unknown
• Estimated regression line:
o Ŷ = β^0 + β^1X Æ least square estimate
ƒ Ŷ = is estimated mean
ƒ β^0 is y-intercept and is constant
• if x = 0, β^0 is the estimated mean value of Y
ƒ β^1 is the slope of the regression line. It is the change in Y when X
is increased by one unit.
7. Least Squares (LS)
• “Best Fit” means difference between actual Y values and predicted Y
values are minimum.

n n
Σ (Yi – Ŷi) = Σ έ2i
i=1 i=1

66
• LS minimizes the Sum of the Squared Differences (SSE)
8. Interpretation of Coefficient
• Slope (β^1)
o The change in the estimated mean value of Y when X is increased by
1 unit
ƒ If β^1 = 0.05, then the estimated mean cholesterol level (Y)
changes by 0.05 mmol/dl when the age is (X) increased by 1 year.
• Y-intercept (β^0)
o Average value of Y when X = 0
ƒ If β^0 = 3.3, then the mean cholesterol level (Y) is expected to 3.3,
when the age (X) is 0 (???)
8. Measures of variation in Regression
• Total variation (Total Sum of Squares (SSTOT))
o Measures variation of observed Yi around the mean Ymean
• Explained variation (Squared Sum of Regression (SSR))
o Variation due to relationship between X & Y
• Unexplained variation (Square Sum of Error (SSE))
o Variation due to other factor
9. Sum of squares
• Total sum of square (SSTOT)
o Measure of total variation in dependent variable Y
o SSTOT = Σ (Yi – Ymean)2 = SSR + SSE
• Regression Sum of square (SSR)
o Measure the variation ‘explained’ by the regression line
o SSR = Σ (Y^i – Ymean)2
• Error Sum of squares (SSE)
o Measures of the ‘unexplained’ variation in Y or the scatter around
the regression line
o SSE = Σ (Yi – Y^i)2

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Measure of variation

Yi
Ŷ = β^0 + β^1Xi
(unexplained sum of squares)
SSE = (Yi – Y^i)2

(total sum of squares)


SSTOT = (Yi – Ymean)2 Y^i

(explained sum of squares)


SSR = (Y^i - Ymean)2

Ymean

X
Xi

Notes:
X, Y and slope:
• Positive slope, Y increases with increase in X
• Negative slope, Y decreases with increase in X

10. Hypothesis Testing:


• For Simple Linear Regression
o H0: β1 = 0 (no linear relationship)
o HA: β1 ≠ 0 (there is linear relationship)
o Test statistics: t-distribution
o Rejection rule:
ƒ Reject H0 if p-value less than 0.05 (assumed )
• For Multi Linear Regression
o H0: β1 = 0 (no linear relationship)
o HA: β1 ≠ 0 (there is linear relationship)
o Test statistics: F-test for ANOVA table:
ƒ F = MSR/MSE
ƒ MSR = SSR/dfReg
ƒ MSE = SSE/dfError

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o Rejection rule:
ƒ Reject H0 if p-value for the F-test less than 0.05 (assumed )
• Assumption
o The errors are normally distributes
o They are independent
o The mean of random error term is equal to zero
o The variance of random error, 2 (sigma square), is constant.
11. How to analyse
• Exploration of the data
o Descriptive
o Scatter plot between two variables
ƒ Check for distribution, relationship and outliers
• Fit the square least line (regression line)
o Using least square method
o It is the best fitting straight line trough the data points in a scatter
plot
o It represents the least square equation and estimates the constant
(a) and slope (b) for  and β Æ Y^ = a + bx
o It is constructed by using the method of least square – minimizes the
sum of squared deviations of each point from the mean (regression
line)
• Evaluation of model by R2 (R square)

Model Summary
model R R2 Adjusted R2 Std Error of the estimate
1 .592a .350 .338 .9043
a. Predictors: (constant), Time
o R2 = 0.35, meaning that 35% of the total variation in GPA is
explained by the study time
o R2 measures the closeness of fit of the sample regression equation to
the observed values of Y
o It ranges fro 0 to 1
o Is called coefficient of determination

69
• Evaluation b
o Evaluation of β using t-statistics
Coefficients
Model Unstandardized Coefficient 95% CI for
B Std error t Sig. Lower Upper
1 (constant) 1.461 .315 4.639 .000 .829 2.093
Time .389 .073 5.342 .000 .243 .534

a- dependent variable for GPA

o H0: β1 = 0 (no linear relationship)


o HA: β1 ≠ 0 (there is linear relationship)
o As p value < 0.001, we reject Ho at 5% significance level and have
sufficient evidence to conclude that there is linear relationship
between study time and GPA.
o Positive β means direct relationship
o Estimated Least Square (LS) equation
ƒ GPA = constant + b (study time)
ƒ GPA = 1.461 + 0.389 (study time)
• Diagnostic checking for assumption
o The assumptions:
ƒ The errors are normally distributes
ƒ They are independent
ƒ The mean of random error term is equal to zero (linearity)
ƒ The variance of random error, 2 (sigma square), is constant or
equal
o Model adequacy checks
ƒ After obtaining the least square line or fit
ƒ Linear model appropriate? ...R2
ƒ Investigate model assumption
ƒ Diagnostic procedures carried out through examination of
Residuals (difference between the observed value Y and the fitted
ot the predicted value Y at a given value X
ƒ Normality

70
• Histogram of unstandardized residuals
ƒ Linearity
• Plot of unstandardised residuals against unstandardised
predicted values
• Creating residual: go to analyse Æ regression bivariate Æ save
Æ unstandardised residual and predicted values
ƒ Let say the assumption is met.
• Interpretation and conclusion
o 35% of the variation in GPA is explained by study time
o There is significant linear association between GPA and study time
o For each 1 hour increase in study, the GPA of a student increase by
0.39
o We are 95% confident that for each 1 hour change in the study tie,
the GPA increase will lie between 0.24 to 0.53

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CORRELATION

1. Introduction
• Correlation is used to measure and describe a relationship between two
variables
• Correlation measure three characteristics of relationship
o The direction of the relationship
ƒ Positive
• It means that when value of one variable increase, the
corresponding value of related variable also increases.
ƒ Negative
• It means that when value of one variable increase, the
corresponding value of related variable decreases.
ƒ Zero correlation (no correlation)
• It means that when values of one variable increases or
decreases independent to the value of other variable
o The form of the relationship
ƒ When value of one variable increases, the corresponding value of
related variable increases or decreases until certain value, but
beyond that value there may have change not in the same trend
or may not have any change at all.
o The degree of the relationship
ƒ It measure how strong the relationship between the values of two
variables
2. Application of correlation
• Prediction
o If two variables are positively or negatively related to each other,
then by knowing the value of one of these variables it is possible to
predict the corresponding unknown value of the other variable
• Validity
o Validity is them measure that a test truly is measuring what it
claims to measure.
• Reliability

72
o Reliability is the measure whether the test instrument produces the
stable, consistent measurements it is used again and again in the
same group of students or people.
• Theory verification
o Theory is a statement that makes a specific prediction about the
relationship between two variables
o This predicted relation can be verified by correlation test
3. Measures of correlation
• Pearson correlation (Pearson product-moment correlation)
o The Pearson correlation measures the degree and the direction of the
linear relationship and is denoted by the letter r (correlation
coefficient)
o r = (degree to which x and y vary together)/(degree to which x and y
vary separately)
o r = (covariability of x and y)/(variabilitiy of x and y separately)
o r = (SP)/√(SSx SSy)
o SP = sum of product of deviation = Σxy – [ (Σx Σy)/n]
o SSx = sum of squared deviation of x = Σxx – [ (Σx Σx)/n]
• Or SSx = Σx2 – (Σx)2/n
o SSy = sum of squared deviation of y = Σyy – [ (Σy Σy)/n]
• Or SSy = Σy2 – (Σy)2/n
• Spearman correlation (Spearman rank-order correlation)
o It is used when the data are of ordinal variable.
o If it is not then data must be ranked
o Rank order the score separately for each variables with 1 for the
smallest score

Case Score for Score for Ranked for Ranked for


no. variable 1 variable 2 variable 1 variable 2
1 3 13 1 2
2 5 14 3 3
3 4 12 2 1

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4 6 15 4 4
5 7 16 5 5

o If there are same score for more than one respondents the final rank
for the respondents will be the average of the ranks

Respondent no Score Rank Final rank


1 3 2 2.5
2 5 5 5
3 2 1 1
4 3 3 2.5
5 4 4 4

o The equitation for the spearman calculation


o rs = 1 – (6ΣD2)/[N(N2-1)]
• N is the number of pair (xy)
• D is the difference between each pair (x – y)
• After calculating the value of r or rs, this is to be compared with the
critical value in the correlation table to decide whether there is
significant correlation between the variables.
o Calculated value Æ tabulated value (significant correlation)
o For one-tailed test df = n-1 and for two-tailed test df = n-2
• df = degree of freedom
o coefficient of determination
• this is squared correlation coefficient
• it measures the percentage of variation shared between the two
variables
• r = 0.40
• r2 = 0.16 i.e. 16%
• Point to be remembered
o Correlation is not causation
o Correlation is affected by the range of data

74
o Correlation is affected by the outliers
4. Hypothesis tests with the Pearson correlation
• Two-tailed
o Ho = ρ = 0 (no correlation)
o HA = ρ ≠ 0 (there is correlation)
• One-tailed
o Ho = ρ ≤ 0 (there is no positive correlation)
o HA = ρ > 0 (there is positive correlation)
• Reporting correlation
o r = 0.65, n = 30, p-value < 0.01, one tail or two tail,
o r2 = coefficient of determination
5. Summary
• Correlation is a statistical test to assess the relation between two
variables
• Relation can be positive or negative
• Two method of test are Pearson and Spearman methods
• Test is used in prediction of relationship testing validity and reliability
and verifying theories
• Can be calculated manually using different formulas or using computer
statistical package like SPSS
• Correlation does not say about cause and effect relationship
• The correlation coefficient is influenced by the outliers and or range of
data under analysis

75
NONPARAMETRIC STATISTICS

1. Nonparametric Statistics (NPS)


• Name nonparametric indicates – no assumption about parameters
(means, variances)
• Require very few assumptions; it is distribution free
• Use median as a measure of central tendency
o Applied when
ƒ The data being analysed is ordinal or nominal
ƒ In case of interval or ratio scale data when no assumption can be
made about the population probability distribution
o Appropriate foe small samples that are not normally distributed
o Computationally easier
o Less efficient than parametric counter parts
o Loose information by substituting ranks in place of scales
Parametric test Nonparametric test
One-sample t test Sign test for one sample
1. Sign test for paired samples
Paired t-test 2. Wilcoxon Signed-Ranked test for
pair samples
Two independent sample t-test Man-Whitney test (Wilcoxon Rank
Sum Test)
One-way ANOVA Kruskal-Wallis Test
ANOVA (randomized block design) Friedman’s Test
Pearson’s correlation coefficient Spearman’s Rank correlation
coefficient

2. Sign Test for Matched


• Observation are matched pairs but assumption underlying the paired t-
test are not met, or the measurement scale is weak then Sign Test can
be applied

76
• Hypothesis
o H0: ∆d = 0 (the median of differences is zero)
o HA: ∆d ≠ 0
• T.S.: smallest of n+ and n-
• RR: Reject H0 if p-value is less than  (assumed alpha)
• Procedure
o Exclude the observations for which the difference (di) is zero
o For di > 0 assign (+sign) and for di < assign (-sign)

3. Wilcoxon Signed-Rank Test for paired samples


• It is sophisticated than Sign test
• Sign test only tell whether the sign of a difference is positive or negative
• This test makes use of both the signs and magnitudes of the differences
• Thus for a strong measurement scale the sign test may be undesirable
since it would not make full use of the information contained in the
data.
• Assumption
o The distribution of difference is continuous
o The distribution of differences is symmetric
• Hypothesis
o H0: ∆d = 0 (the median of differences is zero)
o HA: ∆d ≠ 0
• T.S: T = min (T+, |T-|)
• Rejection region
o Reject H0 if T ≤ critical value or
o Reject H0 if p-value is less than  (assumed alpha)
• Procedure
o Calculate the differences of each pair of observations (di)
o Ignore the signs of these differences
o Rank the absolute values from smallest to largest
o Assign the signs of the corresponding differences to these ranks

77
o A difference of zero is not ranked, it is eliminated from the analysis
and the sample size is reduced by one
o Tied observation are assigned an average rank (suppose two smallest
differences; 4,4; each one will get average rank (1+2)/2 = 1.5)
o Assign each rank either a (+) or (-) sign corresponding to the sign of
the difference
o Compute sum of +ve ranks (T)+ and sum of –ve ranks (T-)
o Choose the test statistics (smallest of T+, |T-|)

4. Wilcoxon Rank Sum Test (Mann-Whitney-U test)


• Counter part of t-test for two independent samples
• Assumptions
o The two samples have been drawn independently and randomly from
their respective populations.
o The measurement scale is at least ordinal.
o The distributions of the two populations have the same general
shape. They differ only with respect to their medians.
• Hypothesis
o H0: the two populations are identical (∆1 = ∆2)
o HA: population 1 and 2 have different medians (∆1 ≠ ∆2)
• Rejection rule:
o Reject H0 if p-value less than 0.05 (assumed )
• Procedure
o Select independent random samples from each population
o Combine the two samples
o Jointly rank the combined samples. If tied observation, assign an
average to all with the same value
ƒ For example: if two observations are tied for the rank 3 and 4
each is given 3.5.
ƒ Next higher value receives a rank of 5 and so on
o Label sample smaller sample size as sample 1. test statistic is the
SUM of RANKS for sample 1, denote region from the table
o Determine rejection region from the table

78
5. Kruskal-Wallis Test
• Counter part of One Way Analysis of Variance (ANOVA: comparing
means of more than two groups) if:
o Normality assumption of ANOVA not justified
o Or the data available is ordinal (consist ranks)
• Assumption:
o The samples are independent and random
o The measurement scale is at least ordinal
o The distribution of the values is sampled populations are identical
except for the possibility that one or more of the population are
composed of values that tend to be larger than those of other
populations.
• Hypothesis
o H0: the two populations are all identical
o HA: At least one of the population tend to exhibit larger values than
others
• Procedure
o If no ties or moderate number of ties the formula simplifies to:
• Rejection region
o When the samples sizes are large (ni ≥ 5) the test statistic T is
distributed approximately as x2 (t – 1)
o Reject H0 if T > x2 (t – 1)

6. Spearman’s Rank correlation coefficient


• Nonparametric alternative of Pearson’s coefficient of correlation
• Relevant when the measurement scale is at least ordinal or the
relationship between two variables is not linear
• It is denoted by rs
• rs = 1 implies strictly increasing monotonicity
• rs = -1 implies strictly decreasing monotonicity

79
NON-PARAMETRIC TESTS

1. Introduction
• Inferential statistics where population parameters are not a requirement
to calculate its value
• A process which is carried out in order to find out whether or not a
particular statistical hypothesis is likely to be true
• A statistical test in which no assumption are made about any statistical
parameter. This is similar to a test in which we do not assume that the
data have any particular distribution
2. The X2 test for goodness of fit
• Test hypothesis about the proportions of a population distribution
• Test how well the sample proportions fit the population proportion
specified by the null hypothesis
• Example:
o Ho : there is no difference in proportion of people in different
categories
o Observed data (fo)

Category 1 Category 2 Category 3


7 26 27
n = 60

o Expected population based on hypothesis (fe)

Category 1 Category 2 Category 3


20 20 20
1/3 of 60 in each category = 1/3 * 60 = 20
o Difference between observed data and expected data (fo – fe)

Category 1 Category 2 Category 3


7 – 20 = -13 26 – 20 = 6 27 – 20 = 7

80
o Square the differences (fo – fe)2

Category 1 Category 2 Category 3


(-13)2 = 169 62 = 36 72 = 49

o X2 = Σ [ (square the difference)/(expected data) ]


o X2 = Σ [ (fo - fe)2/(fe) ] Æ chi square formula
o X2 = 169/20 + 36/20 + 49/20
o X2 = 8.45 + 1.8 + 2.45
o X2 = 12.7
o Degree of freedom = number of column – 1
o Degree of freedom = 3 – 1 = 2
3. Chi-square test for independence
• Test a relationship between two variables
• Each individual in the sample is measured or classified on two separate
variables
• Example:
o Ho : there is no relationship between preference of teaching method
and gender of the students
• Variable 1: teaching method
• Variable 2: gender of students
o Observed data (fo)

Gender Teaching Methods Total


Lecture Tutorial
Male 25 25 50
Female 35 65 100
Total 60 90 150

• 40% students refer lecture


• 60% students prefer tutorial

81
o Expected data (fe)

Gender Teaching Methods Total


Lecture Tutorial
Male *20 30 **50
Female 40 60 100
Total **60 90 ***150

• fe = (**Row total x **Column total)/(***whole total)


• *Example of calculation
o **50 x **60/***150 = *20
o Difference between expected and observed value (fo – fe)

Gender Teaching Methods Total


Lecture Tutorial
Male 25 -20 = 5 25 – 30 = -5 50
Female 35 – 40 = -5 65 – 60 = 5 100
Total 60 90 150

o Square of difference (fo – fe)2


• 52 = 25, (-5)2 = 25, (-5)2 = 25, 52 = 25
o Formula for X2 = Σ [ (fo - fe)2/(fe) ]
• So X2 = 25/20 + 25/30 + 25/40 + 25/60
• X2 = 1.25 + 0.83 + 0.63 + 0.42 = 3.13
• Degree of freedom = (no. Of column - 1)x(no. of row – 1)
• Degree of freedom = (2-1)x(2-1) = 1
• Critical value = 3.84 when  = 0.05 Æ refer to X2 statistic table
o Interpretation
• Calculated values is less than critical value
• Therefore hypothesis is accepted (do not reject the null hypothesis),
there is no difference
o Conclusion

82
• There is no relationship between preference of teaching method and
gender of the students
4. Chi-squared test for variance
• This is a test of the null hypothesis that the population variance is 2.
• We have a sample of size n and we compute an unbiased estimate of the
population variance s2 using divisor n-1.
• The distribution used is X2 statistics is (n-1)2 s2/2.
• We assume that the population is normally distributed
• For 95% level of confidence probability level are within 97.5% and 2.5%
• Find out the critical region in MS Excel CHINV (p, df)

83
STATISTICAL ANALYSIS: WHICH TO CHOOSE?

1. Process of data management (follow the steps below)


• Research question(s)
• Research design
• Data collection
• Data entry
• Data exploration & cleaning
• Data analysis
• Interpretation
• Writing up
2. Role of statistics in a study
• Statistical knowledge and judgment is required at every step of a study
• What statistical analysis is appropriate to answer the research
question? Points to consider to select the right statistical test:
o Research question/ hypothesis
ƒ Are you clear what you want to find out and what design you
have used in your study?
o Number of variables
o Type of data
o Number of groups
o Sample distribution
o Sample type
3. Research question
• The essential question, the study is designed to answer the question
• Most studies are concerned with answering one of four types of
following questions
o What is the magnitude of a health problem or health factor?
o What is the efficacy of an intervention?
o What is the casual relation between one factor (or factors) and the
disease or outcome of interest?
o What is the natural history of a disease?

84
• What is/are the research question (s)?
o Common in medical research:
ƒ Difference between/ among means
ƒ Difference between/ among proportions
ƒ Associations between/ among factors
ƒ Difference between/ among treatment effects
• Hypothesis
o This is a testable statement that describes the nature of the
proposed relationship between two/ more variables interest
o E.g. there is an association between smoking and coronary heart
disease
4. What is the research design applied and expected result?
• Randomized control trial (RCT)
• Observational studies
o Cross-sectional
o Case-control
o Prospective cohort
o Retrospective cohort
• Case report/ series
• Diagnostic test
• E.g. 1
o Research question: effectiveness of new anti-hypertensive drug
o Research design: randomized controlled trial

85
• E.g. 2
o Research question: Risk factor for enteric fever
o Research design: Case control

Time direction

(People with Population


disease)

(People without disease)


• E.g. 3
o Research question: maternal & fetal outcome in mother with PIH
o Research design: prospective cohort

86
Time direction

Population
(no disease)

5. Study factor (s)


• Variable (s) of interest that is hypothesized to be related to health
problem, disease or outcome of interest.
• Also known as the independent variables/ exposure variables/
determinants
6. Outcome factor (s)
• The event or occurrence that is supposed to have as a result of the
study factor
• E.g. the outcome factor is blood pressure, as it influenced by study
factors, salt intake.
• Also known as dependent variable.
7. Number of variables
• One independent variable only – univariate analysis
• More than one independent factor variables – multivariate analysis
• Less likely to conduct and conclude a study with only focusing on
univariate analysis in health sciences
• If there is a multi-factorial effect on the outcome, univariate gives
misleading results
• Example risk factors fro coronary heart disease
• Multivariate analysis can eliminate confounding effect
8. Type of data
• Numerical
o Continuous (e.g. weight)

87
o Discrete (e.g. number of patients admitted)
• Categorical
o Nominal (e.g. occupation, gender)
o Ordinal (e.g. disease severity, socioeconomic status)
• Statistical tests applied are different based on the type of variables
(must consider both independent and dependent variables)
9. Number of group
• Two group (two levels) (e.g. diabetic and non-diabetic group)
• More than two group (more than two levels) (e.g. race – Malay, Chinese,
Indian, Others)
10. Sample distribution
• Normal distribution Æ parametric test
• Non-normal distribution Æ non-parametric test
• Suggested procedure for assessing normality
o Compare the mean & median (for normal distribution mean =
median)
o Construct a histogram overlaid with normal curve
o Construct a box and whisker plot
o Statistical test
ƒ Kolmogorov-Sminov test
ƒ Shapiro-wilk test
• Non-parametric test are appropriate when:
o Data is ordinal
o Data is non-normal distribution and cannot be easily transformed
o Data may contain outlier
• Non-parametric methods have two general limitations
o Not as powerful as parametric counterparts
o Test for complex design are not readily available in standard
computer packages
11. Sample type
• Independent sample (e.g. disease and non-disease groups, male and
female)

88
• Dependent/ paired/ matched sample (e.g. difference of blood pressure
measurements before and after treatment, age and sex matched
samples)
12. What to be asked before choosing a statistical test?
• What is the research question/ hypothesis?
• What is the outcome factor and what are the study factors?
• How many variables?
• How many groups?
• What is the distribution like?
• Are the samples independent?
• Is the data numerical/ categorical?
13. Data exploration and cleaning
• Compulsory to do
• Do not rush to analyze data
• Clean and explore first
• Get acquaintance with the data
• Check duplications
• Out-of-range values and location of error
• Distribution of variables
• Missing data checking consistency errors
• Exploring the relationship between variables
• Transformations
• To get acquaintance with data set before the major analysis is carried
out
o Read the protocol again
o Recall the objectives
o Identify major outcome, exposure and potential confounders/ effect
modifier
o To check records with duplicating ID number (to prevent repeated
data entry
• Error checking
o Respondent’s mis-marking answers

89
o Coder’s miscoding response
o Marking errors by data personnel
• Out-of-range values and location errors
o Measurement error
o Recording error
o Genuine observation
• What to do?
o Check again original measurements where possible
o If original measurements suspicious Æ repeat the measurement
o If not possible to check Æ common sense
o If the value is impossible/implausible Æ justifiable to set as
“missing”
14. Distribution of the variables
o Examine each variable
ƒ Continuous
• Normal distribution
• If not
o ? transformation
o ? categorization
ƒ Categorical
• Frequency distribution
15. Missing data
o Occur when respondent would/could not answer
o Too much missing data
ƒ Threat the study
ƒ Indicate a problem with a question
o Should not be entered as a blank as some statistical packages
interpret blanks as zeroes
o Common practice – coded as 9, 99 or 999
16. Consistency errors
o Situations where respondents answered a question for which they
were ineligible or when codes were entered incorrectly
o Countercheck with questionnaire/data collection form

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o Can be prevented by proper programming in some statistical
software
17. Exploring the relationship between variables
• Cross tabulation useful for categorical variables (sometimes better to
categorize)
• Should consider confounding & interaction
• Graphs – mostly for continuous variables
• Relationship between the outcome variable and other variables
o E.g. scatter plot
18. Transformation
• Severely skewed data – two approaches
o Use nonparametric methods
o Apply transformation
• Many distributions in medicine – skewed to the right
• Involve performing a mathematical operation on every value of the
variable
• Improves the symmetry of the distribution

Transformation Name Effect


X3 Cube Reduce extreme skewness to left
X2 Square Reduce skewness to left
X1/2 Square root Reduce mild skewness to right
log10 (X) Log Reduce skewness to right
-1/√X -ve reciprocal root Reduce extreme skewness to right
Reduce events more extreme
-1/X -ve reciprocal
skewness to right.

• Check the symmetry of the distribution after transformation


• If sufficiently improved Æ use the transformed data
• If resistant to transformation Æ use nonparametric methods
19. Interpretation
• Most confusing part of researchers

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• May be the most difficult part for those who are not familiar with
statistical applications
• Should interpret only when considered to be results of final analysis
stage
o E.g. in multivariate analysis, final model should be interpreted for
writing regardless of the prior more-favorable results towards the
hypothesis
• Recall statistical theory and concepts whenever applicable
• May need help from a medical statistician
20. Univariate analysis
• Test hypothesis between one independent and one dependent variable
21. Multivariate analysis
• Why we need multivariate analysis?
• Purpose of using multivariate analysis
• Common multivariate analysis methods in health sciences research.

Variables Variables

Independent Dependent
Predictor Outcome
Explanatory Response

Covariates
Confounders Not the primary interest
Controls Must be recognized
Effect modifiers

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• Confounding

?
Risk factor Disease

Confounder

o Distortion of a risk factor-disease relationship brought about by


the association of other factors with both risk factor and disease
o Example of confounding:

Physical ?
activity level Systolic BP

Age

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• Interaction

?
Risk factor Disease

Interaction
factor
(effect
modifiers)

o Exist when the primary relationship of interest between a risk


factor and a disease is different at different levels of the interaction
factor
o Example of interaction

Employment ?
in an industry Lung cancer

Cigarette
smoking
Smokers

Risk of
lung
cancer
Non-Smokers

e.g. multivariate analysis


Years of employment in the industry

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Surgery
Compare
outcome

Radiation

• Are these two groups comparable?


• What are the role covariates?
22. Purpose Multivariate Analysis
• To statistically adjust the effect on variable Y by change in a
particular variable x when others are controlled
o X1 Æ Y (X2, X3, X4… statistically adjusted for e.g. diet Æ CHD
{smoking and age adjusted for})
• To discover the variable X which has the most influence on outcome
variable Y

Diet
Smoking
CHD
Age

• To predict the outcome Y

Clinical
Pathology Cancer
Demographic prognosis

Socio-economic

• Whole ideas of multivariate analysis are “How to separate


independent effect of each X and Y”
• Common multivariate analysis methods in health related sciences
research
• Multivariate models

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• Modeling strategies

MTV
Independent Variable Dependent variable
Multiple linear regression >1 1
Multiple logistic regression >1 1
Log-linear regression >1 1
Survival analysis >1 1

Independent variables Dependent variables methods


Continuous Continuous Multiple linear reg.
Categorical Categorical Multiple logistic reg.
Continuous Categorical Multiple logistic reg.
Continuous/ categorical Continuous (survival Survival analysis
time)
Continuous/ categorical Continuous Log-linear analysis

23. Multivariate analysis Æ General Linear Model (GLM)


• The GLM is a flexible statistical model incorporating analysis involving
normally distributed dependent variables and combinations of
categorical and continuous predictor variables.
• The GLM Univariate model procedure provides regression analysis
and analysis of variance one dependent variable by one or more
factors or covariates
• The GLM Multivariate model procedure provides regression analysis
and analysis of variance for multiple dependent variable by one or
more factor or covariates
• The GLM Repeated Measures procedure provides analysis of variance
when the same measurement is made several times on each subject
or case.

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GLM Independent Variable Dependent variable

Univariate GLM ≥1 1
Multivariate GLM ≥1 >1

24. Repeated measures in categorical outcome


• When the dependent variable is a numerical variable

Independent Variable Dependent variable Statistical test


Categorical Numerical Repeated measures ANOVA
(parametric)
Categorical Numerical Friedman test (non-parametric)

• When the dependent variable is a categorical variable

Independent Variable Dependent variable Statistical test


Repeated measure 2 outcomes Mc Nemar’s test
2 measures categories
3++ outcomes Test of marginal Homogeneity
2 measures categories
3++ measures 2 outcomes Cochran’s Q test
categories
Repeated measure Cross-sectional time series (xt)
with independent
variables

Binary
Ordinal Logistic regression
Multiple (xt logic)

97
Count Loglinear regression
(xt poisson)

General estimating equation


(GEE) model (xtgee)

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WRITING A RESEARCH PROPOSAL

1. Introduction:
• Clear statement of the problems or issue to be analysed and the overall
objective of the proposed research.
• Brief summary of relevant studies and literature describing what has
previously been done and what is currently known about the pattern.
• Concise statement of the rationale behind the proposed approach to the
problem

2. Statement of specific research goals:


• List specific objectives
• List specific hypotheses (if any) to be tested
• List the key variables and how they will be operationally defined

3. Study methodology
• Selection of study population
o Size of study population or sample
o Sampling procedure, if any
o Specification of control population, if any
• Description of the experiment or data collection procedure
o Description of research design
o Description of method and intended research tools
o Description of “interfering” (confounding) variables and how they will
be controlled, or how their effects will be evaluated
o If appropriate, a discussion of pitfalls that might be encountered and
of limitations of the procedure proposed
• Diagram of research design (optional): a diagram is useful foe clarifying
points of research strategy
• Analysis plan
o Specify the kinds of data expected to be obtained

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o Specify the means by which the data will be analysed and
interpreted
• Data processing plan
o Hand tabulation or computer
o Analysis technique: statistical measures
o Use of dummy tables
o Test hypothesis or drive hypothesis to meet the objectives of the
study

4. Significance of the research for both practice and theory

5. Time table (Gantt chart)


• Planning phase
• Construction and development of research instruments
• Pre-testing of research tools and techniques
• Selection of population
• Data collection
• Data preparation (coding, editing, cleaning, etc)
• Data analysis
• Report writing

6. Personnel
• Principal investigator
• Assistants
• Supporting persons

7. Facilities available
• Office space
• Resources in field area
• Data analysis equipment
• Other assistance

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8. Collaboration arrangement
• Describe the collaboration

9. Detailed budget
• Personnel
• Consultant fees
• Supplies
• Travel expenses
• Data processing
• Other expenses

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VARIABLES

1. Types of variables
• Continuous or quantitative variables
• Discrete or qualitative variables

2. Continuous or quantitative variables


• Interval-scale variables
o Interval scale data has order and equal intervals.
o Interval scale variables are measured on a linear scale, and can take
on positive or negative values.
o It is assumed that the intervals keep the same importance
throughout the scale.
o They allow us not only to rank order the items that are measured
but also to quantify and compare magnitudes of differences between
them.
o With interval data, one can perform logical operations, add, and
subtract, but one cannot multiply or divide.
o For instance, if a liquid is at 40 degrees and we add 10 degrees, it
will be 50 degrees. However, a liquid at 40 degrees does not have
twice the temperature of a liquid at 20 degrees because 0 degrees
does not represent ‘no temperature’
• Ratio-scale interval
o Finally, in ratio measurement there is always an absolute zero that
is meaningful.
o This means that you can construct a meaningful fraction (or ratio)
with a ratio variable.
o Weight is a ratio variable.
o In applied social research most ‘count’ variables are ratio, for
example, the number of clients in past six months.
o Why? Because you can have zero clients and because it is
meaningful to say that “…we had twice as many clients in the past
six months as we did in the previous six months.”

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3. Qualitative or Discrete Variables
• Discrete variables is also called categorical variables
o Nominal variables
o Ordinal variables
• Nominal variables
o Nominal variables allow for only qualitative classification.
o That is, they can be measured only in terms of whether the
individual items belong to certain distinct categories, but we cannot
quantify or even rank order the categories
o Nominal data has no order, and the assignment of numbers to
categories is purely arbitrary.
o Because of lack of order or equal intervals, one cannot perform
arithmetic (+, -, / or *) or logical operation (<, >, =) on the nominal
data.
o E.g. male and female, unmarried, married, divorce or widower.
• Ordinal variables
o A discrete ordinal variable is a nominal variable, but its different
states are ordered in a meaningful sequence
o Ordinal data has order, but the intervals between scale points may
be uneven.
o Because of lack of equal distances, arithmetic operations are
impossible, but logical operations can be performed on the ordinal
data.
o A typical example of an ordinal variable is socio-economic status of
families.
o We know upper middle is higher than middle but we cannot say how
much higher.
o Ordinal variables are quite useful for subjective assessment of
quality; importance or relevance.
o Ordinal scale data are very frequently used in social and behavioral
research.

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o Almost al opinion surveys today request answers on three-, five- or
seven-point scale.
o Such data are not appropriate for analysis by classical techniques,
because the numbers are comparable only in terms of relative
magnitude, not actual magnitude.
o Consider for example a questionnaire item on the time involvement
by selecting one of the following codes:
ƒ 1 = very low or nil
ƒ 2 = low
ƒ 3 = medium
ƒ 4 = great
ƒ 5 = very great

4. Response variables/target variables


• Often called a dependent variable or predicted variable.
• This is the variable that is being watched and/or measured

5. Explanatory variables/predictor variables


• Any variable that explains the response variable or predictor variable.
• Its values will be used to predict the value of the target variable.
• This is the variable manipulated by the experimenter.

Ratio absolute zero

Interval distance is meaningful

Ordinal attributes can be ordered

Nominal attributes are only name; weakest

6. Confounding variable
• A confounding variable (also confounding factor, lurking variable, a
confound, or confounder) is an extraneous variable in a statistical

104
model that correlates (positively or negatively) with both the
dependent variable and the independent variable.
• Extraneous variables are undesirable variables that influence the
relationship between the variables that an experimenter is examining.
• In other words, confounding is a variable that is associated with the
predictor variable and is a cause of the outcome variable.

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DATA PRESENTATION

1. Two ways of presenting data


• Tables
• Charts

2. Tables
• One-way table (Univariate)
o Table 1: Number of respondents by gender

Gender No. of respondents


Male 51
Female 49
Total 100

• Two-way table (Bivariate)


o Table 2: Number of respondents by gender and their educational
qualification

Gender Primary Secondary Higher Total


Male 15 20 16 51
Female 14 20 12 49
Total 29 40 38 100

Gender Primary (%) Secondary (%) Higher (%) Total (%)


Male 15 ( ) 20 ( ) 16 ( ) 51 ( )
Female 14 ( ) 20 ( ) 12 ( ) 49 ( )
Total 29 ( ) 40 ( ) 38 ( ) 100 ( )

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Gender Primary Secondary Higher Total
(%) (%) (%) (%)
Male 15 20 16 51
() () () ()
Female 14 20 12 49
() () () ()
Total 29 40 38 100
() () () ()

3. Charts
• Charts is a graphically way to organize data
• Types
o Pie chart
ƒ A pie chart is a graphical way to organize data
ƒ All pie charts compare parts of a whole
ƒ A lie chart uses percentages of fraction to compare data
ƒ A type of graph in which percentages values are represented as
proportionally-sized slices of a pie
ƒ Pie charts are especially useful in representing proportions,
percents and fractions.
o Bar chart and Histogram
ƒ A histogram is a bar graph that shows that frequency data
ƒ The first step… collect data and sort it into categories
ƒ Label the data as the independent set or the dependent set
ƒ Data group would be the independent variable and the frequency
of that set would be the dependent variable
ƒ The horizontal axis should be label with independent variable
ƒ The vertical axis should be labeled with the dependent variable
ƒ Each mark on either axis should be equal increments, such as 2,
4, 6, 8, etc
ƒ I think histogram as “sorting bin”

107
ƒ You have one variable, and you sort data by this variable by
placing them into “bins”
ƒ Then you count how many pieces of data are in each bin
ƒ The height of the rectangle you draw on top each bin is
proportional to the number of pieces in that bin
ƒ On the other hand, in bar graph you have several measurement
of different items, and compare them
ƒ The main question a histogram is “how many measurements are
there in each of the classes of measurement?”
ƒ The main question a bar graph answer “what is the measurement
for each item?”

Situation Bar graph or Histogram?


Bar graph.
We want to compare total revenues of
Key question: what is the revenue for
five different companies
each company?
We have measured revenues of several
companies. We want to compare Histogram.
numbers of companies that make from Key question: how many companies
0 to 10,000; from 10,000 to 20,000; are there in each class of revenue?
from 20,000 to 30,000 and so on
Bar graph
We want to compare height of ten oak
Key question: what is the height of
tree in a city park
each tree?
We have measured several trees in a
city park. We want to compare Histogram
numbers of trees that are from 0 to 5 Key question: how many trees are
meters high; from 5 to 10; from 10 to there in each class of height?
15 and so on

o Line graph
ƒ Are more popular than all other graphs combined because their
visual characteristics reveal data trends clearly and these graphs
are easy to create

108
ƒ A line graph is a visual comparison of how two variables – shown
on the x- and y-axis – are related or vary with each other.
ƒ It shows related information by drawing a continuous line
between all the points on a grid.
ƒ Line graphs compare two variables: one is plotted along the x-axis
(horizontal) and the other along the y-axis (vertical)
ƒ The y-axis is a line graph usually indicates quantity (e.g. dollars,
liters) or percentage, while the horizontal x-axis often measures
units of time.
o Scattered plot
ƒ The pattern of the data points on the scatter plot reveals the
relationship between the variables.
ƒ Scatter plots can illustrate various patterns and relationship,
such as:
• Data correlation
• Positive or direct relationships between variables
• Negative or inverse relationship between variables
• Scattered data points
• Non-linear patterns
• Spread of data
• outliers
o Pictograph

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Z-Score & IT’S USES

This is the formula for converting a


given value of x into its corresponding
z score for raw data:

In every normal
distribution 0.3413 of
its total area lies
between the mean and
x = the value that is being standardized z = 1.2
μ = the mean of the distribution
σ = standard deviation of the distribution 0.3413

Z-score for Means

Standard Error formula:

= sample mean
= standard error σ = standard deviation
= population mean n = sample size

1. Z-score serves 2 purposes


• Each z-score will tell the exact location of the original x value within the
distribution
• The z-score will form a standardized distribution that can be directly
compared to other distributions that also have been transformed into z-
scores.

2. Value of z-score
• The sign tells whether the score is located above (+) or below (-) the
mean
• The number tells the distance between the score and the mean in terms
of the number of standard deviation.

110
• The z-score for an item, indicated how far and in what direction, that
item deviates from its distribution’s mean, expressed in units of its
distribution’s standard deviation.
• The mathematics of the z score transformation are such that if every
item in a distribution is converted to its z score, the transformed scores
will necessarily have a mean of zero and a standard deviation and a
standard deviation of one.
• Z scores are sometimes called “standard scores”.
• The z score transformation is especially useful when seeking to
compare the relative standings of items from distributions with
different standard deviations.
• Z scores are especially informative when the distribution to which they
refer, is normal.
• In every normal distribution, the distance between the mean and a
given z score cuts off a fixed proportion of the total area under the
curve.

3. Z-score for making comparison


• For example: bob receive a score of x = 60 on math exam and a score x
= 56 on a biology test. For which course he did well?
o Suppose the biology score had µ = 48 and  = 4 and the math score
had µ = 50 and  = 10.
o Suppose you use a test for your students and the µ = 65 and  = 10
and your friend use a test for your students which have µ = 100 and
 = 15
o Three of your students got 75, 45 and 67 respectively in your test
what should be the score of your students in your friend test if you
want to say the students’ performance in both the tests are same
o Formula for standardized score is x = µ + z
• Second example: Ho = there is no effect of PBL on average score
obtained by the students
o Average score (µ) of USM 3rd year students is 60 with standard
deviation () is 5

111
o A sample of 20 students attended PBL and average score of this
group of students is 65
o Is this increase of 5 marks in average due to chance or the effect of
PBL?
o Answer can be obtained by z test

Standard Error formula:

= sample mean
= standard error σ = standard deviation
= population mean n = sample size

ƒ Ho of the students attended PBL = 60

ƒ H1 of students attended PBL > 60 or ≠ 60


ƒ Level of significance or  level = 0.05 (usually used)
ƒ Z = 1.96

ƒ Z = [sample mean – hypothetical mean]/[standard error between and µ]


ƒ Z = [obtained difference]/[difference due to chance]
ƒ Consult normal distribution table to see if calculated value is in the critical
region or not to reject or accept null hypothesis

112
t-test

1. In calculating z-score we need


• µ = population mean
•  = population standard deviation
• When the standard deviation () is not known, t-test is the alternative.
2. In simple t-test instead of , sample variance is used.
• Sample variance (S2) = [SS/n-1] = [SS/df]
o SS = Σ x2 – ( [Σx]2/n )
ƒ SS = Sum of squared deviation
3. Instead of standard error x, estimated standard error Sx is used.
• Estimated standard error Sx = S/(√n) = √(S2/n)
• t = [X - µ]/Sx
o X = sample mean
o µ = population mean (hypothesis mean)
o Sx = estimated standard error from sample
• The higher the the degree of freedom (df) (sample size) the closure the
S2 (sample variance) to the 2 (population variance)
• Example:
o I prefer PBL than Lecture
ƒ Response Æ 1 = SA, 2 = A, 3 = UD, 4 = DA, 5 = SDA
ƒ From this example hypothesis mean (µ) = 3
ƒ But µ can be getting from study that has been done by someone
previously.
4. Independent measures t-test
• t = [ (X1 – X2) – (µ1 - µ2) ]/ (standard error)
• Pooled variance, S2p = [ SS1 + SS2 ]/[ df1 + df2 ]
• Two samples standard error, Sx1 – Sx2 = √[ (S2p/n1) + (S2p/n1) ]
• Ho = there is no difference in the m=clinical performance of students
attended traditional curriculum and PBL curriculum.

113
SENSITIVITY & SPECIFICITY

1. Definition
• Sensitivity
o Proportion of subject with a target condition who are identified by a
positive test finding.
o Test’s ability to correctly identify individuals with the condition
o Test’s capacity to detect the condition when it is truly present
o Probability of a test being positive given that the condition is present
o Also called true positive rate or hit rate
o The test will actually classify a person (with the condition) as likely
to have the condition
• Specificity
o Proportion of subjects free of the condition who are correctly
identified by a negative test result
o Test’s ability to correctly identify individuals without the condition
o Test’s capacity to exclude condition when it is truly absent
o Also called true negative rate or correct rejection rate
o The test will actually classify a person (without the condition) as
unlikely to have the condition

With the Without the


Respondents Total
condition condition
With the condition a 36 b 96 132
Without the condition c 4 d 864 868
Total 40 960 1000

Sensitivity = a/(a+c) = .90 Æ true positive


Specificity = d/(b+d) = .90 Æ true negative
Positive Predictive Power (PPP) = a/(a+b) = .27 Æ false positive
Negative Predictive Power (NPP) = d/(c+d) = .99 Æ false negative

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2. Validity of the Test

True status (population)


positive negative
Result positive a b
of test negative c d

Sensitivity: the probability of testing positive if the condition is truly


present = a/(a+c)

Specificity: the probability of screening negative if the condition is truly


absent = d/(b+d)

Example: Screening breast cancer by Physical Exam & Mammography


With the Without the
Respondents Total
condition condition
With the condition a 36 b 96 132
Without the condition c 4 d 864 868
Total 40 960 1000

Sensitivity: a/(a+c)
= 36/(36+4)
= 0.90 = 90%
Interpretation Æ screening by physical exam and mammography will
identify 90% of all true breast cancer cases
Specificity: d/(b+d)
= 864/(96+864)
= 0.90 = 90%
Interpretation Æ screening by physical exam and mammography will
correctly classify 90% of all non-breast cancer patient as being free disease.

PPP = a/(a+b)
= 36/(36/96)

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= 0.27 = 27%

NPP = d/(c+d)
= 864/(864 + 4)
= 0.99 = 99%

Validity – the extend to which the test distinguishes between persons with
and without the condition

High validity require


- High sensitivity
- High specificity

116