2nd Asia Pacific Coral Reef Symposium

June 20 – 24, 2010, Phuket, Thailand

ABSTRACTS SUBMISSION FORM

THE APPLICATION OF DNA-BARCODE IN STUDYING NAPOLEON WRASSE

POPULATION RECOVERY IN SERIBU ISLANDS, INDONESIA

Adriani Sunuddin 1,4,*, Irma Shita Arlyza 2, Lilik Litasari 3, Syamsul Bahri Agus 1,4, and Adi
Kurniawan Harahap 4
1*
Department of Marine Science and Technology, Bogor Agricultural University. Jalan
Agatis, Kampus IPB Dramaga, Bogor 16680.
2
Research Centre for Oceanology, Indonesian Institutes for Sciences. Jalan Pasir Putih 1,
Ancol, Jakarta 11048
3
Sub-Bureau for Fisheries and Marine Affairs, Administrative Regency of Seribu Islands,
Provincial Government of Jakarta. Jalan Gunung Sahari 11, Jakarta 10720.
4
Ocean Imperator: centre for marine research. Jalan Ketapang 50 RT/RW 01/01, Lancang
Island, Kepulauan Seribu 14430.
E-mail*: adriani@ipb.ac.id

The napoleon wrasse, Cheilinus undulatus Ruppell, 1835 (Family Labridae), is an

endangered fish species and among the most important species in the global live reef food
fish trade. Indonesian reef waters serve as the major source of production and resulted in its
population decline including in the coral reef ecosystem of Kepulauan Seribu. Recent
sporadic sightings and capture of napoleon wrasse in Kepulauan Seribu since 2008 signal
its population recovery. A total of 70 fish were sampled from fishers and two culture
facilities, showing skewed distribution of size frequency with modal size peak at 21.0 to
30.9 cm. Size frequency indicated that napoleon wrasse population was at juvenile stage.
DNA barcode for napoleon wrasse was produced for 5 specimens, extracted from fin
tissue, each represents different sampling localities, i.e. Panggang Island, Pramuka Island,
Pari Island, Harapan Island, and Kelapa Island. Compromising our results of DNA-barcode
with internet-based BLAST search available at Barcode of Life Data System (BOLD)
website (www.barcodinglife.org), we revealed taxon confirmation for four specimens with
Cheilinus undulatus and one unidentified species for another specimen obtained from
Panggang Island. Further analyses revealed sister lineage of the unidentified specimen with
outgroup species from Lutjanidae, indicating the possibility of two distinct populations of
Cheilinus undulatus were available in Seribu Islands. However, complete resolution the
napoleon wrasse population recovery in Seribu Islands requires the addition of sequences
from more obtained tissue samples.

Keywords: napoleon wrasse, Cheilinus undulatus, Seribu Islands, DNA barcode

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Your abstract falls within the following subject area (please specify and rank your choices)

 Bioactive Compounds  Evolution and Systematic

 Biogeochemical Cycles in Coral Reefs
 Oceanography and Remote Sensing
 Modeling for Conservation of Coral
Reefs
 Reef Monitoring and Assessment
 Database and Information System
 Dinoflagellate Biology and Coral
Symbiosis
 Biology and Ecology of Coral Reef
Organisms
 Coral Biology and Physiology
 Coral Reproduction and Recruitment
 Disturbances on Coral Reefs
 Coral Reef Genetics
 Diseases on Coral Reefs
 Biodiversity of Reef Organisms
x Reef Fish Biology and Ecology
 Reef Associated Fisheries
 Reef Connectivity
 Marine Protected Areas
 Reef Restoration
 Climate Change and Reef Resilience
 Social-ecological Systems
 Management and Conservation of Coral
Reef Ecosystems

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