Brian E. Applegate, Ph.D.

Education

Ph.D. in Chemistry, December 2000

Ohio State University, Columbus, OH 43210
Dissertation: “Experimental and Theoretical study of the Jahn-Teller Effect in the
Cyclopentadienyl Radical and the Benzene Family of Cations”
Advisor: Terry A. Miller
M.S. in Chemistry, June 1998
Ohio State University, Columbus, OH 43210
Thesis: “The Spectroscopy and Dynamics of Transient Radicals: The
Perdeuteromethoxy radical”
Advisor: Terry A. Miller
B.S. in Chemistry, June 1994
Wright State University, Dayton, OH 45435
cum laude

Professional Experience

09/06-present Assistant professor, Dept. of Biomedical Engineering, Texas A&M University,

College Station, TX
09/03-09/06 Postdoctoral Fellow, Dept. of Biomedical Engineering, Duke University,
Durham, NC
08/00-08/03 Postdoctoral Fellow, Dept. of Chemistry, University of North Carolina at Chapel
Hill, Chapel Hill, NC
consulting: Bioptigen Inc., Durham, NC
List of Publications (reverse chronological order)

Book Chapters
1. A. Oldenburg, B. E. Applegate, J. A. Izatt, S. A. Boppart, “Molecular OCT Contrast
Enhancement and Imaging,” In Optical Coherence Tomography: Technology and
Applications, J. Fujimoto, W. Drexler, Eds. (Springer: New York, NY, USA, 2008).
2. B. E. Applegate, and J. A. Izatt, "Molecular Contrast OCT," in Optical Coherence
Tomography in Cardiovascular Research, E. Regar, A. M. G. J. v. Leeuwen, and P. W.
Serruys, eds. (Informa Healthcare, 2007).
Journal Articles (peer reviewed)
1. J. A. Jo, B. E. Applegate, J. Park, S. Shrestha, P. Pande, I. B. Gimenez-Conti, and J.
L. Brandon, "In-Vivo Simultaneous Morphological and Biochemical Optical Imaging of
Oral Epithelial Cancer," IEEE Trans Biomed Eng in press(2010).
2. J. Park, J. A. Jo, S. Shrestha, P. Pande, Q. Wan, and B. E. Applegate, "A dual-
modality optical coherence tomography and fluorescence lifetime imaging microscopy
system for simultaneous morphological and biochemical tissue characterization,"
Biomed. Opt. Express 1, 186-200 (2010).
3. S. Shrestha, B. E. Applegate, J. Park, X. Xiao, P. Pande, and J. A. Jo, "High-Speed
Multispectral Fluorescence Lifetime Imaging Implementation for in vivo applications,"
Optics Letters 35, 2558-2560 (2010).
4. D. Jacob, R. L. Shelton, and B. E. Applegate, "Fourier domain pump-probe optical
coherence tomography imaging of Melanin," Optics Express 18, 12399-12410 (2010).

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 5. R. L. Shelton and B. E. Applegate, "Off-Axis Photoacoustic Microscopy," IEEE Trans
Biomed Eng 57, 1835-1838 (2010).
6. Q. Wan, and B. E. Applegate, "Multiphoton coherence domain molecular imaging with
pump-probe optical coherence microscopy," Opt. Lett. 35, 532-534 (2010).
7. E. J. McDowell, A. K. Ellerbee, M. A. Choma, B. E. Applegate, and J. A. Izatt, "Spectral
domain phase microscopy for local measurements of cytoskeletal rheology in single
cells," Journal of Biomedical Optics 12, 044008-044001-044012 (2007).
8. B. E. Applegate and J. A. Izatt, "Molecular imaging of endogenous and exogenous
molecular chromophores with ground state recovery pump-probe optical coherence
tomography," Optics Express 14, 9142-9155 (2006).
9. M. V. Sarunic, B. E. Applegate, and J. A. Izatt, "Real-time quadrature projection
complex conjugate resolved Fourier domain optical coherence tomography," Optics
Letters 31, 2426-2428 (2006).
10. B. E. Applegate, C. Yang, and J. A. Izatt, "Theoretical comparison of the sensitivity of
molecular contrast optical coherence tomography techniques," Optics Express 13,
8146-8163 (2005).
11. M. V. Sarunic, B. E. Applegate, and J. A. Izatt, "Spectral domain second harmonic
optical coherence tomography," Optics Letters 30, 2391-2393 (2005).
12. B. E. Applegate, C. Yang, A. M. Rollins, and J. A. Izatt, "Polarization resolved second
harmonic generation optical coherence tomography in collagen," Optics Letters 29,
2252-2254 (2004).
13. W. K. Lewis, B. E. Applegate, J. Sztaray, B. Sztaray, T. Baer, R. J. Bemish, and R. E.
Miller, "Electron impact ionization in helium nanodroplets: Controlling fragmentation by
active cooling of molecular ions," J Am Chem Soc 126, 11283-11292 (2004).
14. C. Yang, L. E. L. McGuckin, J. D. Simon, M. A. Choma, B. E. Applegate, and J. A.
Izatt, "Spectral triangulation molecular contrast optical coherence tomography with
indocyanine green as the contrast agent," Optics Letters 29, 2016-2018 (2004).
15. B. E. Applegate, T. A. Barckholtz, and T. A. Miller, "Explorations of conical
intersections and their ramifications for chemistry through the Jahn-Teller effect,"
Chem Soc Rev 32, 38-49 (2003).
16. B. E. Applegate and T. A. Miller, "Calculation of the Jahn-Teller effect in benzene
cation: Application to spectral analysis," Journal of Chemical Physics 117, 10654-
10674 (2002).
17. C. J. Burnham, S. S. Xantheas, M. A. Miller, B. E. Applegate, and R. E. Miller, "The
formation of cyclic water complexes by sequential ring insertion: Experiment and
theory," Journal of Chemical Physics 117, 1109-1122 (2002).
18. B. E. Applegate, A. J. Bezant, and T. A. Miller, "The Jahn-Teller and related effects in
the cyclopentadienyl radical. II. Vibrational analysis of the A2A2''- X2E1'' electronic
transition," Journal of Chemical Physics 114, 4869-4882 (2001).
19. B. E. Applegate, T. A. Miller, and T. A. Barckholtz, "The Jahn-Teller and related effects
in the cyclopentadienyl radical. I. The ab initio calculation of spectroscopically
observable parameters," Journal of Chemical Physics 114, 4855-4868 (2001).
20. M. B. Pushkarsky, B. E. Applegate, and T. A. Miller, "Photofragmentation dynamics of
the thiomethoxy radical," Journal of Chemical Physics 113, 9649-9657 (2000).
21. B. E. Applegate, M. B. Pushkarsky, and T. A. Miller, "Spectral analysis and
photofragmentation dynamics of the perdeuteromethoxy radical," J Phys Chem A 103,
1538-1546 (1999).
22. A. P. Salzberg, B. E. Applegate, and T. A. Miller, "Rovibronic Spectroscopy of MgCH3
A2E <-- X2A1 Transition," J Mol Spectrosc 193, 434-441 (1999).
23. B. E. Applegate, M. C. Yang, and T. A. Miller, "Competition between radiation and
photofragmentation in the A2Σ+ state of the SH/D rare gas complexes," Journal of

Brian E. Applegate 2
 Chemical Physics 109, 162-169 (1998).

Patents

1. C. Yang, B. E. Applegate, D. K. Rao, M. A. Choma, S. Yazdanfar, A. M. Rollins, J. A. Izatt,

Method for optical coherence tomography imaging with molecular contrast. 7,075,658, July
11, 2006, USA.
2. C. A. Toth, J. A. Izatt, B. E. Applegate, P. McKinley, P. Challa, L. W. Herndon, S.
Freedman, B. Dodge, R. Nappi, Ophthalmic instruments, systems and methods especially
adapted for conducting simultaneous tonometry and pachymetry. pending
3. M. V. Sarunic, B. E. Applegate, J. A. Izatt, Quadrature projection full range high speed
Fourier domain optical coherence tomography. pending

Academic Service

Member, Steering Committee, Spectroscopy Institute at The Ohio State University, 1999
Reviewer, Journal of Biomedical Optics, Journal of the Optical Society of America, Optics
Express, Biomedical Optics Express, IEEE Transactions on Biomedical Engineering, Lasers in
Surgery and Medicine, Optics Letters.
Member, Alternate chair, CLEO 10: Medical and Biological Applications Subcommittee, for
the Conference on Lasers and Electro-Optics (2007-2009)
Chair, CLEO 10: Biophotonics and Optofluidics, for the Conference on Lasers and Electro-
Optics (2009-present)
Member, National Advisory Committee, 16th International Conference on Luminescence
(2010-present)

Professional Affiliations

American Chemical Society, member (1994-present)

Optical Society of America, member (2003-present)
The International Society for Optical Engineering (SPIE), member (2003-present)
Society for Molecular Imaging (2009- present)

Active Research Grants

NIH/NIBIB, 1R21EB007729-01A1
New technologies for high-resolution optical molecular imaging

NIH/NCI, 1R21CA132433-01A2
Combined OCT/FLIM biochemical and anatomical imaging for early cancer diagnosis

NIH/NCRR, 1R21RR025799-01
Development of High-Resolution Molecular Imaging of Endogenous Chromophores with
Pump-Probe Optical Coherence Microscopy

DOD
Diagnosis and Treatment of Blast-Induced Hearing Loss

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