DAFTAR ISI

Halaman

HALAMAN JUDUL................................................................................................ii
LEMBAR PENGESAHAN ....................................................................................iii
LEMBAR PERNYATAAN ....................................................................................iv
ABSTRAK ...............................................................................................................v
ABSTRACT .............................................................................................................vi
KATA PENGANTAR .............................................................................................vii
DAFTAR ISI ............................................................................................................ix
DAFTAR GAMBAR................................................................................................xi
DAFTAR TABEL.....................................................................................................xii
DAFTAR LAMPIRAN............................................................................................xiii
DAFTAR ISTILAH DAN LAMBANG..................................................................xiv

BAB 1 PENDAHULUAN
1.1 Latar Belakang.........................................................................................1
1.2 Rumusan Masalah....................................................................................3
1.3 Batasan Masalah.......................................................................................4
1.4 Tujuan Penelitian......................................................................................5
1.5 Manfaat Penelitian....................................................................................5
1.5.1Manfaat Akademis..........................................................................5
1.5.2 Manfaat Praktis..............................................................................5

BAB 2 TINJAUAN PUSTAKA

2.1 Rattus norvegicus Galur Wistar...............................................................6
2.2 Diabetes Melitus yang diInfeksi Staphylococcus aureus................................7
2.2.1 Diabetes Melitus.............................................................................7
2.2.2 Staphylococcus aureus...................................................................13
2.3 Streptozotosin...........................................................................................16
2.4 Limpa........................................................................................................17
2.5 Superoksida Dismutase (SOD).................................................................19
2.6 Nuclear Factor kappa B (NF-KB).............................................................21

BAB 3 KERANGKA KONSEP DAN HIPOTESIS

3.1 Kerangka Teori.........................................................................................23
3.1 Kerangka Konseptual...............................................................................26
3.2 Hipotesis Penelitian..................................................................................27

BAB 4 METODE KEGIATAN

4.1 Waktu dan Tempat Penelitian..................................................................28
4.2 Alat dan Bahan ........................................................................................28
4.2.1 Alat.................................................................................................28
4.2.2 Bahan..............................................................................................29
4.3 Tahapan Penelitian...................................................................................29

ix
 4.4 Prosedur Kerja..........................................................................................30
4.4.1 Rancangan Penelitian.....................................................................30
4.4.2 Persiapan Hewan Coba...................................................................32
4.4.3 Induksi Streptozotosin....................................................................33
4.4.4 Pengukuran Kadar Gula Darah......................................................33
4.4.5 Pembuatan Suspensi dan Induksi Staphylococcus aureus.............34
4.4.6 Nekropsi dan Preparasi Organ Limpa............................................34
4.4.7 Flow Cytometry..............................................................................35
4.4.8 Analisis Data..................................................................................35

BAB 5 HASIL DAN PEMBAHASAN

5.1 Hasil Penelitian Induksi Streptozotosin dan Staphylococcus aureus
terhadap Terjadinya Diabetes Melitus Infeksi Staphylococcus aureus
.................................................................................................................36
5.2 Pengaruh kombinasi streptozotosin dan Staphylococcus aureus pada
pembuatan hewan model diabetes melitus yang diinfeksi
Staphylococcus aureus terhadap kadar relatif SOD................................41
5.3 Pengaruh kombinasi streptozotosin dan Staphylococcus aureus pada
pembuatan hewan model diabetes melitus yang diinfeksi
Staphylococcus aureus terhadap kadar relatif NF-KB.............................47

BAB 6 KESIMPULAN DAN SARAN

6.1 Kesimpulan ..............................................................................................52
6.2 Saran ........................................................................................................52

DAFTAR PUSTAKA...............................................................................................53

LAMPIRAN..............................................................................................................59