Retno Wahyuningrum
Fakultas Farmasi
Universitas Muhammadiyah Purwokerto
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Uji aktivitas penghambatan terhadap Mycobacterium tuberculosis dengan metode Microplate Alamar Blue
Assay (MABA)
Prinsip Metode Microplate Alamar Blue Assay
KHM
Rifampisin
KHM
Isoniazid
Inkubasi hari ke 5, sesaat setelah penambahan Alamar Blue Inkubasi hari ke 6, 24 jam setelah penambahan Alamar Blue
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Bioautography
Konsentrasi uji
• quantities higher than 1 mg/ml for extracts or 0.1 mg/ml for isolated
compounds should be avoided
• whereas the presence of activity is very interesting in the case of
concentrations below 100 µg/ml for extracts and 10 µg/ml for isolated
compounds
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Mekanisme aktivitas
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Referensi
• Radulovi, N.S., Blagojevi, P.D., Stojanovi, Z.Z., Stojanovi, N.M., n.d.
Antimicrobial Plant Metabolites: Structural Diversity and Mechanism of
Action 22.
• Silva, N., Fernandes Júnior, A., 2010. Biological properties of medicinal
plants: a review of their antimicrobial activity. J. Venom. Anim. Toxins incl.
Trop. Dis 16, 402–413. https://doi.org/10.1590/S1678-
91992010000300006
• Wink, M., 2015. Modes of Action of Herbal Medicines and Plant
Secondary Metabolites. Medicines 2, 251–286.
https://doi.org/10.3390/medicines2030251
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TERIMA KASIH
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