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ABSTRAK

AWALUDDIN. Identifikasi Jamur Dermatofit pada Penderita Dermatofitosis


Kontak Hewan dengan Metode Kultur dan Polymerase Chain Reaction –
Restriction Fragment Length Polymorfism (PCR – RFLP) dengan Enzim MvaI
(Dibimbing oleh Rizalinda Sjahril dan Faridha Ilyas).

Penelitian ini bertujuan mengidentifikasi jenis jamur dermatofit pada


penderita dermatofitosis yang sehari-hari sering kontak dengan hewan ternak
maupun dengan hewan peliharaan dengan metode kultur dan PCR-RFLP.
Penelitian ini merupakan penelitian observasional laboratorium dengan
desain penelitian deskriptif kategorik.
Pengambilan sampel dilakukan di Balai Kesehatan Kulit dan Kelamin,
beberapa Sekolah Dasar di Kota Makassar, Kartini Medical Center dan salah
satu Panti Jompo di Kabupaten Gowa. Dari 130 populasi pasien
dermatofitosis diperoleh 23 sampel yang memiliki riwayat kontak dengan
hewan ternak dan hewan piaraan untuk di identifikasi dengan metode kultur
dan metode PCR-RFLP. Sampel diidentifikasi dengan metode PCR dengan
menggunakan primer ITS 1 dan ITS 4 dilanjutkan pemotongan dengan enzim
restriksi Mva I. Untuk metode kultur, kerokan yang positif berdasarkan uji
KOH 20%, diinokulasikan pada media Saboraud Agar dan di inkubasi pada
suhu 37oC pengamatan dilakukan setiap 3 hari sekali selama 30 hari,
kemudian koloni jamur diamati secara makroskopik dan mikroskopik.
Dengan metode kultur, pada 23 pasien hanya ada 5 sampel pasien
(21,7%) yang tumbuh dan dapat diidentifikasi. Kelima sampel positif tersebut
adalah Microsporum canis (40%) dan Microsporum audounii (60%). Dengan
metode PCR-RFLP semua sampel teridentifikasi yakni M. canis 26,1%,
Trichophyton rubrum 13,1%,T. mentagrophytes 21,6%, T. tonsurans 8,7%, T.
verrucosum 4,2% dan spesies unclassified 26,1%.

Kata Kunci : Dermatofitosis, Jamur Dermatofit, PCR – RFLP, Metode Kultur.

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ABSTRACT

AWALUDDIN. Identification of Dermatophytes on Dermathophytoses Patient


with Animal Contact by Culture and Polymerase Chain Reaction – Restriction
Fragment Length Polymorfism (PCR – RFLP) with MvaI Enzymes (supervised
by Rizalinda Sjahril and Faridha Ilyas).

The aims of the research was to identify the types of dermatophytes on


dermathophytosis patients who frequently had contact with farm animals or
pets using culture and PCR-RFLP methods.
This research was an observational laboratory study with categorical
descriptive research design. The samples were taken from dermatophytosis
patients who visited the Kartini Medical Centre, The Skin Health Venereal
and Cosmetic Center and dermatophytosis in schools children in primary
schools in Makassar and one of the Nursing Homes in Gowa District. Of the
130 dermathophytosis patients as the population, 23 samples had a history of
having contact with farm animals and pets for identification with culture and
PCR-RFLP methods. The samples were identified with PCR method using
ITS1 and ITS 4 primer continued with cutting with Mva I restriction enzyme.
For culture method, a positive test was based on KOH 20%, inoculated on
the Saboraud Agar and incubation at temperature 37 oC. Observation was
done once every three days during 30 days. The fungal colony was observed
macroscopically and microscopically.
The result indicate that with culture method there are 23 patients
identified, but only 5 samples (21,7%) that showed fungal growth on
Saboraud Agar who grow and can be identified. The 5 positive sample are
Microsporum canis (40%) and Microsporum audounii (60%). By using PCR-
RFLP all samples were positive dermatophytosis. Comprising Microsporum
canis 26,1%, Trichophyton rubrum 13,1%, T. mentagrophytes 21,6%, T.
tonsurans 8.7%, T. verrucosum 4, 2% and unclassified species 26,1%.

Keywords: Dermatophyte, Dermatophytosis, PCR – RFLP, Culture method.

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