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College Student Participation in Research Projects, National Science Council, Taiwan 2006/04

Correlation between androgen receptor CAG repeat length


polymorphism and the risk of prostate cancer in Taiwan population

Yu-Hsiang Chen1 , Hsing-Hui Wang1, Kuan-Chou Chen2, Hsiu-Mei Hsieh1


1
Department of Life Science, National Taiwan Normal University
2
Department of Urology, Taipei Medical university Hospital, Taiwan

Abstract

Androgen plays a major role in early stage of prostate cancer. Many studies have

indicated that the length of CAG repeat in exon 1 of the androgen receptor gene is

associated with the risk of prostate cancer in different ethnicities. Different length of

CAG repeats may form different protein structure, and then influence the affinity with

androgen, which in turn to have differential transactivity for the downstream target

genes. In this study, we analyzed the length of CAG repeats in 118 samples, including

57 patients with prostate cancer and 61 controls (benign prostate hyperplasia patients

were used as control group).

We extracted patients’ and controls’ DNA from paraffin embedded tissues then

amplified DNA by using Polymerase Chain Reaction (PCR). After verifying the PCR

products, we detected the CAG repeats by using automated MegaBACE Analyzer.

For statistical analysis, we used Student’s test and odds ratio. In prostate cancer

patients, the length of CAG repeat is 22.4+/-2.84 (mean +/- standard deviation); in

controls, the length of CAG repeat is 22.95+/-2.78. There was no significant

difference identified between these two groups (p=0.29). Men with short CAG

repeats (n < 23) had 1.72-fold increased risk for prostate cancer compared to those

with long CAG repeats (n > or = 23) by odds ratio, but no significance (odds ratio, 1.72;

95% confidence interval, 1.65-1.78) was identified.


Special Topics in Bioinformatics, NTNU, Taiwan 2006/02

Discovery of Biomarkers and Drug Targets in Human Breast Cancer by


Proteomics Approach

Yu-Hsiang Chen1, Cheng-Kang Pai 1, Hsueh-Fen Juan2


1
Department of Life Science, National Taiwan Normal University
2
Department of Life Science and Institute of Molecular and Cellular Biology, National Taiwan University

Abstract

Breast cancers are potentially life-threatening malignancies in Taiwan. The

patients always suffered from chemotherapy and surgery. In order to improve their

quality of life, finding out a new approach for medications is truly necessary.

Currently, people are trying to use proteomics, a high-throughput method, is a

powerful tool, to select specific protein markers which are able to provide useful

information for drug design. In this study, we tried to find out biomarkers of breast

cancer for diagnosis in early stage. Hopefully, based on the new selected markers, the

newly designed drugs will help current medications for breast cancers.

We obtained four sets of breast cancer tissues and normal breast tissues from

Department of Oncology, National Taiwan University Hospital. After separating

proteins by two dimensional electrophoresis, we selected the points with different

expression level on the corresponding spots between cancer and normal groups. We

extracted the proteins by using in-gel digestion and these proteins were identified by

using MALDI-Q-TOF in Institute of Biological Chemistry, Academia Sinica.

Preliminarily, we found that b-Actin, protein disulfide isomerase, a-enolase, and

vimentin were up-regulated; transferrin was down-regulated in the tumor tissue.

These proteins are associated with energy metabolism, protein folding, cytoskeleton

structure and cell communication. We are going to find out the roles of these

proteins in tumor development and try to identify them as potential biomarkers for

developing novel drugs for breast cancer.

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