Proteomic

Reference Cells Analysed Features

Strategy
50 spots were found to be
differentially expressed on
induced and uninduced MPRO
cell lines of which 28 were
identified using MS and
correlated to pI and Molecular
weight these were GRP78,
Lian, Z. et MPRO 2D- PAGE Cytoplasmic Gamma Actin,
al (2001) (murine BCR- and MS, Proliferating cell nuclear antigen,
(28) ABL cell line) MS/MS APS kinase, Pyrubate kinase 3,
Melanoma X-actin,
Glyceraldehyde-3-phosphate
dehydrogenase, Stefin 3, Guanine
nucleotide binding protein,
Triosephosphate isomerase,
Testis-derived c-abl protein,
RNA binding motif protein etc.
Compared cell lines pre and post
K562, KU812, imatinib treatment for tyrosine
Goss, V. et Phospho
SUP- phosphorylated sites. Found 188
al (2006) proteomic,
B15,BV173,K nonredundant tyrosine
(29) LC-MS/MS
CL22,SD1 phosphorylated sites of which 77
are novel.
2D-PAGE was carried from pH
3-11. 84 protein spots were
different totaling 44 unique
proteins. They further classified
the proteins as 1. Heat Shock
CML cell lines Proteins and Chaperones 2.
LAMA 84 S Nucleic Acid Binding/
Ferrari, G. (imatinib Synthesis/Stability. 3. Structural
2D-PAGE,
et al (2006) sensitive) and Proteins. 4. Cell Signaling. 5.
MS
(30) LAMA 34 R Metabolic Enzymes. They
(imatinib believe that further investigations
resistant) of the protein expression
signature of CML cells are
warranted to allow the
development of additional
molecular targeted therapeutic
agents.
Balabanov, K562 2D-PAGE, Investigated the effects of
S. et al MS imatinib on the protein
(2007) (31) expression profiles of BCR- ABL
positive cells. Found eIF5A a site
of hypusination representing the
 transfer of an amino-butyl residue
to lysine to be down regulated in
imatinib sensitive cells. Identified
inhibition of eIF5Ahypusination
as a promising new approach for
combination therapy in BCR-
ABL positive leukemias
Hantschel, Chemical Found Tec kinases Btk and Tec
O. et al K562 Proteomics, are prominent targets for
(2007) (32) LC-MS/MS dasatinib inhibition.
Compared CML and APL cell
lines for differences in plasma
Lee, S. J. et K562 and
LC- membrane proteins by
al (2007) AML cell line
MS/MS concentrating these using a
(33) NB4
biotin- avidin affinity purification
system
2D PAGE analysis of proteins in
cells was performed according to
O’Farrel (14), with a slight
modification. In brief,cells (2 3
107) were solubilized in 400 ml
of lysis buffer containing 9.5 M
Nunoi, H et
Human urea, 2% NP40, 2% Pharmalyte
al (1999) 2D-PAGE
Neutrophils (1.6% pH 5–8 and 0.4% pH 3–
(34)
10), and 5% mercaptoethanol.
Isoelectric focusing was
performed at 500 V for 24 hours
followed by the 2D SDS PAGE.
Proteins on the gel were stained
with 2D Silver Stain
Identified 191 protein spots
corresponding to 142 different
proteins of which 63% were
cancer-related proteins. Detailed
analysis of 35 differentially
Fontana, S CML cell lines
2D-PAGE/ expressed proteins revealed that
et al (2007) LAMA84,
MS LAMA84 cells preferentially
(35) K562, KCL22
expressed proteins associated
with an invasive behavior, while
K562 and KCL22 cells
preferentially expressed proteins
involved in drug resistance.
Govekar, R. Tumors of the 2D-PAGE/ Cancer proteomic research
et al (2009) Gingivo- MS-MSMS conducted in India. 40 ug of
(36) buccal protein was used for 2D-PAGE
complex on pH 4-7 strips (Bio-Rad)
followed by silver staining. Spots
were compared using Mann-
Whitney’s test and those with
significant differences in
expression and/or with tumor to
normal ratios of 2 and above or
0.5 and below were taken as
‘differentiators’